LOCUS       NZ_CP070373            21053 bp    DNA     linear   CON 18-MAY-2024
DEFINITION  Streptomyces sp. MBT27 chromosome, complete genome.
ACCESSION   NZ_CP070373
VERSION     NZ_CP070373.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MBT27
  ORGANISM  Streptomyces sp. MBT27
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S.S., Hankemeier,T. and van
            Wezel,G.P.
  TITLE     Discovery of novel glycerolated quinazolinones from Streptomyces sp.
            MBT27
  JOURNAL   J Ind Microbiol Biotechnol 46 (3-4), 483-492 (2019)
   PUBMED   30729343
REFERENCE   2  (bases 1 to 8400223)
  AUTHORS   Machushynets,N., Elsayed,S., Carrion,V.J., Siegler,M.A., de la
            Cruz,M., Genilloud,O., Hankemeier,T. and van Wezel,G.P.
  TITLE     New from old: conjugation of actinomycin X2 and anthranilamide to
            form the novel actinomycin L in Streptomyces sp. MBT27
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8400223)
  AUTHORS   Du,C., Machushynets,N. and van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2021) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   4  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S., Hankemeier,T. and Van
            Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2020) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 1.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011751035.2-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 03:23:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,575
            CDSs (total)                      :: 7,482
            Genes (coding)                    :: 7,345
            CDSs (with protein)               :: 7,345
            Genes (RNA)                       :: 93
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 39 of 137
            Pseudo Genes (incomplete)         :: 114 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:14:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4865005
            Orig. end                         :: 4886058
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MBT, Institute of Biology
            Leiden, Leiden University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21053
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="22"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21053
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21053
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11053](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11053)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    complement(629..643)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
     misc_feature    861..875
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.16"
                     /tool="antismash"
     gene            complement(942..1811)
                     /locus_tag="GWQ54_RS21420"
     CDS             complement(942..1811)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676882.1"
                     /locus_tag="GWQ54_RS21420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_167160702.1"
                     /transl_table=11
                     /translation="MDDWYVDDRCTNCDVARQFAPELIGETGGKSQILRPPSGAEETRR
                     LHAAVFACPTRSIRPRSGRADQSRDPFPMELDDGLWICGHNSRHTAGANSYLLLRPTGT
                     AMMIDTPRWSAELADRYAASGPVTDVLLTHRDHAAHGRSYADHFGARLWIHEGDLDAAP
                     DADCVIRGIDPVEIAEGVTVHPLPGHTRGSVLYLADDRYCFSGDSFYWSRATGDLEVAD
                     SVTWYSIEEQAVSLARTAERLRFEWVLPGHGDRHRLPADEMSRRLRALTERTRLLEPRP
                     VDFTAVRW"
     gene            complement(2013..3953)
                     /locus_tag="GWQ54_RS21425"
     CDS             complement(2013..3953)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514817.1"
                     /locus_tag="GWQ54_RS21425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_167160700.1"
                     /transl_table=11
                     /translation="MSAPQRERHPRPAGPVPALGQAEATLVEHYPRLVRLAYLILPPRL
                     GRHRRVLAAHGVVQNALPARPTPIKKGRVPAPRDDRAPALGWIVRRVVGAALSYEHGAA
                     RWRRAVRPALPAVWGLRLTPRIGGADELALEQQLATVPAGVRAAFVLQHLEGYASDTAA
                     ELLTAAGVPDPQAALRAAKALGPQDDGTRAGLLLDSVEFDPCTVQARPTDLLRRRHWRR
                     WGAAAALLVAVMLTVGQSAKDPASPHPTTPSAVGVAMVDPGAVGRAPGTAWQSSSRIDF
                     TVWPARGSLVDDRALLGRALRAWARPATAVQVSAAPRTGTGPTAASPQLLYAGRVDDAD
                     VVLLYDGERVVRYAETTGQPAELAFSRADNADVTTAAALVLSRTGSRTRFLFAPWVAES
                     TTRDLLAPDSPARALHVGDDGVSDAVARPDLTGPCSNWPALQLRSSERIAEKHAFLLTD
                     LGDLSPVHLTYTPPPGGGVPARQPREATSTAALVSWSHTACTLRSAVGGGVRSVNNWVF
                     AEQQLPENAGQAEWLCSRADTWNGPGRAVVRFQPPTTAAREPGTLIADARDTSYCSRFN
                     QHILADARWSAPSGASYLLAAGSRAVTRITVTGQVRADTTGTTLAVKAPPGQSAEIGAV
                     LSTGRTLGAVH"
     misc_feature    complement(3956..3970)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.42"
                     /tool="antismash"
     gene            4466..4822
                     /locus_tag="GWQ54_RS21430"
     CDS             4466..4822
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /locus_tag="GWQ54_RS21430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VOC family protein"
                     /protein_id="WP_167160698.1"
                     /transl_table=11
                     /translation="MRTTHIMANLPVADIATAKSFYRDYLGLSTEEFDLTWVARCTSPD
                     SAASVQLVTHDATALEDSVISVLTDDVEGAYAEARRLGFEIVHPLTTEPWGHRRFFVRA
                     PDGNVINIGFHGDA"
     gene            5075..5263
                     /locus_tag="GWQ54_RS21435"
     CDS             5075..5263
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GWQ54_RS21435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167160696.1"
                     /transl_table=11
                     /translation="MYEMHVGTAVTGTRRVWHVVAHDQRATLCGQPLDPVEDTQTDHHC
                     LPCMSAFQRLMQAVEPA"
     gene            complement(5641..5814)
                     /locus_tag="GWQ54_RS21440"
     CDS             complement(5641..5814)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995073.1"
                     /locus_tag="GWQ54_RS21440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CsbD family protein"
                     /protein_id="WP_100573672.1"
                     /transl_table=11
                     /translation="MSGEEKGKSKVEQAKGKVKEAAGRAVGNERLEAEGRGEQAKGDAR
                     QAKEKIKDVFKH"
     gene            6056..6217
                     /locus_tag="GWQ54_RS21445"
     CDS             6056..6217
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031579.1"
                     /locus_tag="GWQ54_RS21445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrophobic protein"
                     /protein_id="WP_167160694.1"
                     /transl_table=11
                     /translation="MVPLLLVLLLALILFGAGFALKILWWVAIVVLVLWLVGFVARPRG
                     GSSRWYRW"
     gene            complement(6496..7353)
                     /locus_tag="GWQ54_RS21450"
     CDS             complement(6496..7353)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140318.1"
                     /locus_tag="GWQ54_RS21450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="WP_161249320.1"
                     /transl_table=11
                     /translation="MRTAEPETGHEVRVLPVIEDPARVAPRDARALSRQFFERLAVLEE
                     GTAEYQYARNTLIEMNMSLVRFAARRFRNFADEMEDVVQVGMIGLIKAIDRFELSRETE
                     FATFAVPYIVGEMKRFFRDTTWAVHVPRRLQERRSELARAREELESRLDRSPTVKELAA
                     LMDLSEDEVVEAQMAANGYNSASLDAALSGDEESGEAALADFIGAEDPAMDLVENFQSL
                     APLMAELSDRERLLLQLRFGEELTQSQIGEELGVSQMQVSRLLSRTVAKLRAGMFETAT
                     GATA"
     gene            7697..8662
                     /locus_tag="GWQ54_RS21455"
     CDS             7697..8662
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118137.1"
                     /locus_tag="GWQ54_RS21455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="WP_167160692.1"
                     /transl_table=11
                     /translation="MSPRRPAPAPLPRLHRIELDGTGPEDVVFDHAGRVLTGLGDGRLL
                     RIELPGRPGGPADLVEIGRTGGRPLGLDVLADGRLLVCDAQRGLLRFDPDGTAGFDVLA
                     DSVAGQPLRFCSNAVAASDGTVYFTVSSRRYGLADWPSDIVEHRPTGLLVRLTPGGEPE
                     VLLDGLQFANGVALAPDESFVAVAETGARRLTRWWLTGAEAGRHDTFARDLPGFPDNMS
                     RSPDGGFWVALAGPRTPPLDWLHRTAPPVRRAAAAALRLLPPLPAHAAGVVEVDVRGRG
                     IRQLADTGRQFRMATGVAERDGRLVLGSLVEDAIAWCDIR"
     gene            complement(8756..9919)
                     /locus_tag="GWQ54_RS21460"
     CDS             complement(8756..9919)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240938.1"
                     /locus_tag="GWQ54_RS21460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="oxygenase MpaB family protein"
                     /protein_id="WP_167160690.1"
                     /transl_table=11
                     /translation="MTYTEASMDALRQAGDELADATVATLFERGEVGKFNTLMRYVSTV
                     GAPLPDGLPDVAREYLQVTSAPPPWVDWGEMERARLFFIDNNVHISTALSFASMPACYV
                     VPHVAKLLSATHGLKYPSKRMAETGQFTVYLMQPDAFEAGSRFIPAAQKVRLLHASIRH
                     HLKRENRWDTGTLGTPICQEDMIGGQMFFSLLVLDSLHRLGIHMSTEGAEAYYYAWRVV
                     GAMLGVDQEAVPKSLEEAREFLDLYMVRHMAPSDEGAELTRQLIDLYEEIVPGTFFDPI
                     VSALIRHLVGDTCADWLQVPRTRWDTVVKAVPHLLGVLETIEDRSPLGAWALDRIGHLT
                     AVLELSSLTRGRVMHYAIPEQLKKDFGVAGAVARTHRWTPPTATVPS"
     gene            complement(10001..11053)
                     /locus_tag="GWQ54_RS21465"
     CDS             complement(10001..11053)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031263.1"
                     /locus_tag="GWQ54_RS21465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_167160688.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.1e-36, bitscore:
                     125.1, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDRWEPAAFKARVDEVLRHFVAAEADELSDIDPLLGPVAGQLEA
                     AVADGKRLRSAFCYWGWRAVGQPDSDALVRAAASMELVHAAAVVHDDLIDDSPLRHGRP
                     SAHIALRGAVRRRPRAVAAARSLAMLVGDLLMSLAGQLFATSGLPAAYLARARPLWSLL
                     ARELIAGECLEILRTGAPPDISASLKVIRYKTAKYTVEQPLLIGAALAGAGERLRQGYS
                     AYGLPLGEAFQLRDDLLGLFGDPERTGKANSDDVRGHRPTALLAETWRLADAGERERLG
                     SLLGRRDLDEEGLAEVRAVMRSLKAPERIELMIGERVDEALTVLGTLNAPAPATAALTA
                     LARSAAIRLS"
     misc_feature    complement(10724..10726)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(11046..11321)
                     /locus_tag="GWQ54_RS21470"
     CDS             complement(11046..11321)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379395.1"
                     /locus_tag="GWQ54_RS21470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase"
                     /protein_id="WP_167160686.1"
                     /transl_table=11
                     /translation="MTGEAGRREGLDEQAVLLAAGIADLAVSTLGSALGVVRGLLRRSD
                     TAEMAADAEHDLLARGRLVVDRYAAAPPAHLEVLARHALARRAGDV"
     gene            11575..12345
                     /locus_tag="GWQ54_RS21475"
     CDS             11575..12345
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /locus_tag="GWQ54_RS21475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="WP_205380424.1"
                     /transl_table=11
                     /translation="MIPSHVDDTLPELAPFLHEVRAHRPDDGTWCEAWTVRDVLAHQTG
                     NAEELARVLEAFLAGSPVETRGFDREEPYRRMSDPDLWAAFMSRCEHLAEIAAAAERDL
                     SPDTEIMWTGRSVNVPFFAEHMREELILHRWDLSGDDRTAVQALAEPWMTEHSVVSVGR
                     PLLVRGSTGLDLGPDGRIEGRLRCPGTDDVVVTADAGGTGIRLAAPEGPATIESDAATR
                     VLLLWGRRPADPSRWSSSAGPDSLRRVRTLLSGY"
     gene            complement(12353..12772)
                     /locus_tag="GWQ54_RS21480"
     CDS             complement(12353..12772)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627071.1"
                     /locus_tag="GWQ54_RS21480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cupin domain-containing protein"
                     /protein_id="WP_229860991.1"
                     /transl_table=11
                     /translation="MIVLGTTRLRLLEDGSNTGHRLGIAESVLAPHTPGPPQHRHAQHD
                     EGFYVISGTVRFTVRDRDYDATAGTLVMVPPGAPHTFANVTDQPAVMLSTFTPDLYVQY
                     FRDLQDMVAGGRAMTPEATIRTMSRYATEPAADFG"
     gene            12963..13859
                     /locus_tag="GWQ54_RS21485"
     CDS             12963..13859
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529059.1"
                     /locus_tag="GWQ54_RS21485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR substrate-binding domain-containing protein"
                     /protein_id="WP_167160682.1"
                     /transl_table=11
                     /translation="MELRTLRYFVAVAEELHFGRAARRLHMSQPPLSRAIKQLETDLGA
                     SLLDRSPLGVALTPAGAVLLDEARALLLGADRVRGRVARAAGTATLTLGILGDSADPRV
                     LRLADAYRRRHPHVEVRVREADLSDPTCGLHAGTVDVALTRGPFYETGLTVHELRTDPV
                     GALLRTDDPLAGRVALTLADLADRRWFVFPEGTDPRWQAYWNGGEPREGPVVRAVQECR
                     QAVLWNGTVGLTLLDHDPGESLTVVPLTDMPPSRLVMAWRESAPAPLVHSLARTAAAVH
                     GAGVRGVSDLGAAVRGA"
     gene            14059..15195
                     /locus_tag="GWQ54_RS21490"
     CDS             14059..15195
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472382.1"
                     /locus_tag="GWQ54_RS21490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_167160680.1"
                     /transl_table=11
                     /translation="MRSRNVLISGASVAGLTLAHWLQRAGFRPTVVERAPGLRRGGQAI
                     DIRGSALGVVERMGLLDKVRSATTDMRGMSFVDARGKELSRSTEATLTGGLIDNDDVEI
                     MRDDLTTFLFDTSRYGTEYLFGDVITSVEQNEDTVEVRFRHERPRSFDLVIGADGLHSN
                     VRRLAFGPESDFLHFLGSYLAIFSVQNYLGLDHWQTYHTSDDLLVGVYSVRQNTEARAM
                     LLFDSPELDYDHRDLDQQKSILGERFAGVGWETPRLLESMRDAPDFYFDSMSQVHLDHW
                     SNGRVGLVGDAGYCASPRSGQGTSIAMVGAYVLAGELANHPHDHRSAFTRYQDLMRDYV
                     RDNQRLAQNRAADTNRTPPDELARAANSITLPDWPHLG"
     misc_feature    14212..14214
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(15247..16971)
                     /locus_tag="GWQ54_RS21495"
     CDS             complement(15247..16971)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088719.1"
                     /locus_tag="GWQ54_RS21495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_167160678.1"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 2.9e-18, bitscore:
                     65.3, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADALLFNPHTYDPKHFDPETRRLLRATVDWFEQRGKRRLIEDYR
                     SRAWLADFLAFSAKEGLFATFLTPASAAGDGEGDKRWDTARIAALNEIFGFYGLDYWYA
                     WQVTILGLGPVWQSDNAAARTRAAELLAQGEVFAFGLSEKTHGADIYSTDMLLEPDGDG
                     GFRATGSKYYIGNGNAAGLVSVFGRRTDVEGPDGYVFFAADSRHEAYHLVKNVVDSSKY
                     VSEFRLENYPVRAEDVLHSGRAAFDAALNTVNVGKFNLCTASIGICEHAMYEAVTHAQN
                     RILYGRPVTAFPHVRRELTDAYVRLVGMKLFSDRAVDYFRSAGPEDRRYLLFNPMTKMK
                     VTTEGEKVIDLMWDVIAAKGFEKDNYFAQAAVEIRGLPKLEGTVHVNLALILKFMRNHL
                     LNPADYAPVPTRLDAADDAFLFQQGPARGLGSVRFHDWRTAYDAFAELPNVARFREQAD
                     ALCEFVTTAAPDEEQSRDLDLLLAVGQLFALVVHGQLILEQARLTDLDADVLDELFAVL
                     VRDFSAHAVELHGKDSATSEQQGWALGAVRRPVVDAARSARVWDRVEALSGTYEMAP"
     gene            complement(17104..17658)
                     /locus_tag="GWQ54_RS21500"
     CDS             complement(17104..17658)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1278: PadR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016640749.1"
                     /locus_tag="GWQ54_RS21500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_167160676.1"
                     /transl_table=11
                     /translation="MALEHAILVSLLEKPGSGYELARRFDRSIGYFWAATHQQIYRILK
                     RMEGDGWIDAREVAQAGRPDKKEYSVADPGRAALSRWLHEPIEPESVRHELAVKIRGAA
                     FDDPAALLHEVERHRRAHADRLAHYLAGELRDFTGPEAPAPDPGRELQHVVLRGGIAYE
                     RMTLAWLDDVIATLHRLGAER"
     misc_feature    17743..17755
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     gene            17826..18341
                     /locus_tag="GWQ54_RS21505"
     CDS             17826..18341
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GWQ54_RS21505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_240361798.1"
                     /transl_table=11
                     /translation="MHIPGPELRTDGGAGAIAAAGGLHHYQRLLHAAYGPVVRFQPPGA
                     ETAVSIADPVLLEATAHLDERPERLFDFLTPLFEAGNVQVLPVEEHTPWRRLLLSVLAG
                     RPSHERHFARFTELTTAPADSWSEGAHAGTDREPVALQKDLGDGLRRLPGAVGPGRRVR
                     NSPRSRRS"
     gene            18338..18466
                     /locus_tag="GWQ54_RS38080"
     CDS             18338..18466
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GWQ54_RS38080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="cytochrome P450"
                     /protein_id="WP_229860922.1"
                     /transl_table=11
                     /translation="MILAILLKRFRFRPVPGHKVVPIERFVLWAADDIRMTVRRRE"
     gene            complement(18517..19167)
                     /locus_tag="GWQ54_RS21510"
     CDS             complement(18517..19167)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1156: Chloramphenicol acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125376.1"
                     /locus_tag="GWQ54_RS21510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="WP_167160674.1"
                     /transl_table=11
                     /translation="MPVPADSTVLHPMPGQPRVVLLKPLVTSPLIEVGEYSYYDDPDDA
                     TAFETRNVLYHYGPEKLIIGRFCALGTGVRFIMNGANHRMDGPSTFPFPTMGGSWAEHF
                     DLLTGLPNRGDTVVGHDVWFGHGATVMPGVRIGHGAIIAAGAVVTSDVPDYGIVGGNPA
                     RLIRSRYSEPDVARLLAVGWWDWPAEFITEHVRTIMSGSIDDLEAAASKSPTA"
     misc_feature    19274..19285
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.36"
                     /tool="antismash"
     gene            complement(19303..19953)
                     /locus_tag="GWQ54_RS21515"
     CDS             complement(19303..19953)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106095.1"
                     /locus_tag="GWQ54_RS21515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_167160672.1"
                     /transl_table=11
                     /translation="MAAHGATNEQLGRRERKKAQTRRTIAETALRLFLERGYDAVGVRE
                     VAQEADVATATLFAHFPSKEALVFDQDDEQEEALLDVVRTRPEGVSIPEALRRWFHGMV
                     DAARNEPDLDRFHRLIETTPALRDYSARMWLRHEQALANVIAADIGLAAPDTASRLVAR
                     TALGVIPSITSLDEGARIIDVTFDFVEAGWTATAPAADGGATAGGSQDRACAR"
ORIGIN
        1 cgcgcgggac tggacaccgt ggtctgcgag gccgacgcgt tcgccgcccg ggccgcacgc
       61 gagcggatcg ccgtctcgct cgaacgggcc gtgcagcgag gaaagttggg ccgcggtgcg
      121 gccgaggaca cactggcccg catggtcttc accggcagtc tcgacgacct ggccgaccgc
      181 cagctcgtca tcgaggccgt cgtcgagaac accgcggtca agacagaggt cttcgcccgc
      241 ctcgacaaga tcgtcgaaga cccggacgcg atcctcgcca ccaacacgtc ctccatcccc
      301 gtcatgcgcc tgggcatggc cacccggcgc gccgaccgtg tcctggggct gcacttcttc
      361 aaccccgtac cggtgctgcc gctggtcgag atcgtgtcct cgctgcacac cacgtcgcgg
      421 acgaccgatg ccgtggcggc cttcgccgag cacaccctgg gcaagacggt cgtacgttct
      481 caggaccggg cggggttcgt cgtcaacgcg ctcctcgtgc cctacctcct gtcggcgatc
      541 cgcatgacgg agtccggctt cgccaccccg gccgatgtgg acacggccat ggagctgggc
      601 tgcgcccacc cgatgggccc gctgaagctg gccgatctga tcggcctgga cacggtcgcc
      661 gccatcgcgg aatccctcta cgaagagttc aaggaacccc tctacgcccc gccgcccttg
      721 ctgcagcgca tggtggaggc agggctgctc ggccgcaaga gcggccgcgg gttccacctc
      781 tacgaccggg gctgaggggc ccgggtcggc cgccggacgg agggccggcg gccgctgtgg
      841 agaggggcgt ggaacggatt gggagatccg ttccacgcct cactcgtcca cgccttgcac
      901 cgccacgcct cctcgcccgc tcgcccgcac caccgtcacc gtcaccagcg gacggcggtg
      961 aagtcgaccg gccggggttc gagcagccgg gtgcgttccg tcagcgcgcg cagccggcgg
     1021 gacatctcgt cggcgggcag ccggtggcgg tcgccgtggc ccggcaacac ccactcgaag
     1081 cggagccgct cggccgtcct cgccagcgaa acggcctgct cctcgatgga gtaccaggtg
     1141 acgctgtccg ccacctccag gtccccggtg gcccgcgacc agtagaagct gtcaccgctg
     1201 aagcagtacc tgtcgtccgc gaggtagagc acgctccccc gggtgtggcc gggcagcggg
     1261 tggacggtca ccccctcggc gatctccacg gggtcgatcc cccggatcac acagtcggcg
     1321 tccggggccg catcgaggtc tccctcgtgg atccacaggc gggcgccgaa atggtcggcg
     1381 taactcctgc cgtgtgcggc atggtcccgg tgtgtcagca ggacgtccgt caccggcccc
     1441 gacgccgcgt atcgatcggc gagttcggcg ctccagcgcg gggtgtcgat catcatcgcc
     1501 gtgccggtgg gccgcagcag gaggtaggag ttggccccgg cggtgtgccg ggaattgtgc
     1561 ccgcagatcc acagaccgtc gtccaactcc atcggaaacg ggtcgcggct ctggtccgcc
     1621 cgcccggacc tgggccggat cgaacgtgtc gggcaggcga agacggcggc gtgcaggcgc
     1681 cgtgtctcct ccgcaccgct gggcgggcgc aggatctgcg acttccctcc ggtctcgccg
     1741 atcagctcgg gcgcgaactg gcgggcgaca tcgcagttgg tgcaacggtc gtccacgtac
     1801 cagtcgtcca tggcagtgct ccttcgacag agggggcgag cgggacacgg gccgtcaccg
     1861 gtccgccaac tcccctacct cgcttgctga ttgcagtggt cgcgtggcca agcatgcaca
     1921 cgcgaccggc catatcgcac atggccgtcc gcttgcgatt gcggcgaggt gctgctctgc
     1981 gcgacctcgg cgggatgccg ctccggaccg cctcaatgaa cagcgccgag cgtccttccc
     2041 gtgctgagca ccgcgccgat ttccgccgac tggcccgggg gcgccttcac ggcgagggtc
     2101 gtgccggtcg tgtcggcgcg cacctggccc gtcacggtga tccgcgtgac ggcgcggctt
     2161 cccgcggcca ggaggtagga ggcgcccgag ggagcgctcc agcgggcgtc ggccaggatg
     2221 tgctggttga aacggctgca gtacgaggtg tcccgcgcgt cggcgatcag ggtgccgggc
     2281 tcgcgcgccg ccgtcgtggg cggctggaac cgtacgacgg cccggcccgg accgttccag
     2341 gtgtcggccc tcgaacacag ccactccgcc tggcccgcgt tctccggcaa ttgctgttcc
     2401 gcgaagaccc agttgttgac cgaccgtacg ccgcccccca ccgcggaccg caacgtacag
     2461 gccgtgtggg accagctcac cagcgccgcc gtgctcgtgg cctcgcgggg ctgacgggcc
     2521 gggacgccgc cgccgggcgg cggcgtgtag gtgaggtgga cgggtgacag gtcaccgagg
     2581 tcggtgagca ggaaggcgtg cttctcggcg atgcgctcgg acgaacgcag ctgcagggcg
     2641 ggccagttgg agcagggccc ggtgaggtcg gggcgagcca ccgcgtcact gacgccgtcg
     2701 tcgccgacgt gcaatgcgcg ggccgggctg tcgggggcga gcaggtcgcg cgtggtggat
     2761 tcggccaccc agggggcgaa caggaagcgt gtccggctcc cggtccggct gaggacgagc
     2821 gcggccgccg tggtgacatc ggcgttgtcg gcgcgcgaga aggcgagttc ggccggctgc
     2881 ccggtggttt ccgcgtagcg cacgacccgc tcgccgtcgt acaggaggac cacgtcggcg
     2941 tcgtccaccc ttcccgcgta caggagttgg ggcgacgcag ccgtcggtcc cgtcccggtc
     3001 ctgggcgcgg ccgacacctg gacagcggtg gccggccgcg cccaggcacg gagcgcgcgc
     3061 cccagcaggg cccggtcgtc gaccaggctg ccgcgcgcgg gccagaccgt gaagtcgatg
     3121 cgcgagctcg actgccaggc cgtgcccggc gcgcgcccca cggcccccgg gtcgaccatc
     3181 gcgacgccga cggccgaggg ggtggtggga tgcggcgacg ccgggtcctt ggcgctctgg
     3241 cccaccgtga gcatcaccgc gacgaggaga gcggcagccg ccccccagcg ccgccaatgg
     3301 cgacggcgca acaggtccgt gggccgggcc tgcacggtgc acgggtcgaa ctcgacggag
     3361 tcgagcagca gccccgcccg ggtgccgtcg tcctgcgggc ccagggcctt ggcagcgcgc
     3421 agcgcggcct gcgggtccgg gactccggcc gccgtcagca gctcggcggc ggtgtccgag
     3481 gcgtacccct ccaggtgctg cagcacgaag gccgcccgga cccctgccgg tacggtcgcc
     3541 aactgctgct ccagggccag ttcgtccgca ccgccgatac ggggtgtcag cctcagcccc
     3601 cacacggcgg gcagcgcggg ccgcacggcc cggcgccagc gggcggcacc gtgctcgtag
     3661 gaaagggccg cacccaccac gcggcgcacg atccagccga gggccggcgc ccggtcgtcg
     3721 cgcggcgcgg gcacccggcc cttcttgatc ggcgtcggcc gggcgggcag cgcgttctgt
     3781 accacgccgt gcgcggcgag cacgcgccga tggcggccga gccggggagg aaggatgagg
     3841 taggccagcc ggacgagccg ggggtagtgc tcgacgagcg tcgcctccgc ctggccgagc
     3901 gcgggcaccg gaccggcagg acgggggtga cgctcgcgtt gtggggctga catggtggaa
     3961 caccttctcc ggggcggacg ttgtggcttt gcccttcaaa cgagcgaatc ggatgacagt
     4021 cacaccgcga gcgcctcttg ggggcgtgcg ggtgccttcc gggcgccgta cgcgcctcgc
     4081 gcatcctccc ggggcagtcg ctcgttcccc cttccatgga agagtgcgct catccgatca
     4141 aaggcccgcg cgtgacgaag cgttgttcct ccccgccgga ggaggccggc gcccgacccc
     4201 cgcgaaacga gagcccgcca cccgcgaacg cgcatgcgca gtgcgggccg ggcgggagcg
     4261 acgtgagctt caaccggtgg ggcacggtgg tgttctgacg gccatgcgcg cgcccgcgcc
     4321 atggcggaac gccactcccc cgtcctgtcg ggtacggaat catcgcgagc cagaagaggc
     4381 cgacggcggt cggtgcgcgc gcccacgtcg gcccgtgtca ccgcgatccg ccgtcgcccg
     4441 tctctcctac gagcgggagg accgcatgcg taccacgcac atcatggcca atcttccggt
     4501 ggccgacatc gcgacggcca agagtttcta ccgggactac ctcggcctga gcaccgagga
     4561 gttcgatctg acatgggtgg cccgctgcac atcgcccgat tccgcggcga gcgtccagct
     4621 cgtgacgcac gacgcgacgg cgctcgagga ctcggtcatt tccgtcctga ccgatgacgt
     4681 cgaaggcgct tacgccgagg cgcggcggct cgggttcgag atcgtgcatc cgctgaccac
     4741 ggagccgtgg ggccaccgtc gcttcttcgt ccgggcgccg gacggcaacg tgatcaacat
     4801 cggattccac ggcgacgcgt gacggccgcg gagaccctgg agggcttgga gaccccgaag
     4861 gggctggagg ccctggggac ctcggagagc cggccccgaa accccgggca ggcgccgcgg
     4921 taccgggccg cggacggcgg tgcgaggcgg cgtcagggac cggtgtccgc atcctaatcg
     4981 cggcggctcc ggattcaccc gaatagcgtc ctgaacaggc gtgggcccct caggggtggt
     5041 ggtgtgaagt gctctccccc gtaggaggac cgccatgtac gaaatgcacg tcggcacggc
     5101 cgttaccggc acccgccggg tgtggcacgt cgtcgcccac gaccagcgcg ccaccttgtg
     5161 cggccagccc ctcgaccccg tcgaggacac tcagaccgac caccactgtc tgccctgcat
     5221 gtcggccttc cagcgcctca tgcaagccgt cgagcccgcc tgacggtatt cggcatcgag
     5281 ggcgtgcccg ccctccggag gcgtgatgga ccgcatctgc ctgacatgtt catcgaagaa
     5341 atcggcggca tcggagcccg cgaggccgtc caccgcgatc ttcaccttgt tcgtgctttt
     5401 ccaggtgacc tggggtcgca ggggccgcct cgcactggct ctgcgtcggc cgtacggggc
     5461 gacgtaggcg ttgccgccgc ctctgtcccg cgcccgtccc aagcccgtcc caagaacgat
     5521 cgcgtccggg gtgccgccac ggacgcgaca cgaggacgag ggcgggaccg accgaatcag
     5581 ccggccggcc ccgcccccgc gccctgaccc gctcggtcag cgcgtcgagt accagtgcgc
     5641 tcagtgcttg aagacgtcct tgatcttttc cttggcctgg cgggcgtcac ccttggcctg
     5701 ctcgccgcgg ccctcggcct caaggcgctc gttgcccacg gcgcggccgg cggcctcctt
     5761 gaccttgccc ttggcctgct cgaccttcga cttgcccttt tcctcaccgg acacaacaac
     5821 caccactccc acaaatcgac gtcaaactgc gacagcagta ctcctcgccg cattcggccg
     5881 atccaaaccg cagagaccca aaggaagtcc accgccttct cccagcgtac gggattgcgc
     5941 cagaattccg gagggcgagg cgattcgggc gcttgagcca gggaatatgt atcaagccga
     6001 ttcgagtgcg tgacgggtgt cttccgggca cgcggtggac tggaggttga aagccatggt
     6061 tccccttctt ctcgtgctgc tgctcgccct gatccttttc ggcgcgggct ttgccctcaa
     6121 gatcctttgg tgggtggcca tcgtggtcct ggtcctgtgg ctcgtcggtt tcgtcgcccg
     6181 ccccaggggc ggcagctccc gctggtaccg ctggtagccg agcggcacgc agaccacgca
     6241 gacataggac agcagacata ggacaggggc cggaccttga gcaggtctcc ggcccttgtt
     6301 ctctgtcatt tggcgtacgt cttctgtcgt ataccttcgg ctgccgtatg ccgtcggcat
     6361 cgatgccgac gggagacgaa aaaggaagcc gacgggatga atcaggactg gcctgaattc
     6421 aaagcccggt cgacttcctt ttcctcattc gttcaacgtc cggttgtcct ggatcgcact
     6481 gtcccggacg gcacttcagg cagttgcgcc cgtggcggtc tcgaacattc cggcgcggag
     6541 cttggcgacg gtacgcgaca gaagccggga gacctgcatc tgggaaacgc ccagctcttc
     6601 accgatctgg ctctgggtga gctcctcgcc gaagcgcagc tgcagcaaca ggcgctcccg
     6661 gtcggagagt tccgccatca gcggggcgag gctctggaag ttctccacca ggtccatcgc
     6721 cgggtcctcg gcaccgatga agtccgccag ggccgcttcc ccgctctcct cgtcgcccga
     6781 gagcgcggcg tccagggagg cggagttgta accattggcc gccatctgcg cctcgacgac
     6841 ctcgtcctcg ctgaggtcca tgagggcggc gagttccttg acggtgggcg agcggtccag
     6901 acggctctcg agctcctcgc gggcccgggc cagctcactg cggcgctcct ggaggcgtcg
     6961 cggcacgtgg acggcccagg tggtgtcacg gaagaagcgc ttcatctcgc cgacgatgta
     7021 gggcaccgcg aaggtcgcga actcggtctc gcgggagagc tcgaagcggt cgatcgcctt
     7081 gatcaggccg atcatgccga cctggaccac gtcctccatc tcgtccgcga aattgcggaa
     7141 gcgacgagcg gcgaaccgca cgagggacat gttcatctcg atcagggtgt tccgcgcgta
     7201 ctggtactcg gccgtgccct cctcaaggac cgcgaggcgc tcgaagaact gccgggacag
     7261 cgcccgcgca tcgcgaggag ccacgcgcgc cgggtcctcg atcaccggaa gcacccgcac
     7321 ctcgtgtccg gtctccggct cggcggtcct catcgtcgca gtcgctacgg cagtcacagc
     7381 cactcccctc ctcgtccccc gtacaaataa cattcgtttc gcgtctgccc cgaactcacc
     7441 gatccattcg cacgagattc ggacccgttt cccggcggtc ccgccgaggc cgtgtccacc
     7501 gcccagccat gggttttcgg cgcgcgggaa cggtcacccg tgccgggggc ccgtaccagg
     7561 cggacgcact cgcgccgccg gatcacacgg gccccggagt ccccggaacg atacgcgcga
     7621 agcttggaca cctgtccgcg atgagaaggg ccgccatgcc gactcccacc ggaacccgcc
     7681 tcgccagggg tggcgcatga gccctcggcg ccccgccccg gccccgctgc cgaggctgca
     7741 ccgcatcgag ctcgacggca ccggccccga ggacgtcgtc ttcgatcacg cgggccgggt
     7801 gctcacgggg ctcggggacg gccgcctcct gcggatcgag ctgcccgggc gtcccggcgg
     7861 acccgccgac ctcgtggaga tcggccgcac cggcgggcga ccgctcgggc tcgacgtcct
     7921 cgccgacgga cggctgctgg tgtgcgacgc ccaacgaggc ctgctccgct tcgatcccga
     7981 cggcacggcc gggttcgacg tgctggccga ctcggtggcc gggcaaccgc tgcggttctg
     8041 cagcaacgcg gtggcggcgt ccgacggcac cgtctacttc acggtgtcca gccgccggta
     8101 cggcctcgcc gactggccga gcgacatcgt ggagcaccgc ccgacaggcc tgctcgtccg
     8161 tctaaccccg ggcggggagc ccgaggtcct cctggacggg ctgcagttcg ccaacggggt
     8221 ggctctcgcc cccgacgagt cgttcgtcgc ggtggccgag accggagcgc gccggctcac
     8281 ccggtggtgg ctgaccggtg ccgaagcagg ccgtcacgac acgttcgcac gggacctgcc
     8341 gggcttcccc gacaacatgt cccgcagccc ggacggcggg ttctgggtcg cgctcgccgg
     8401 tccccgcaca ccgcccctgg actggctcca ccggaccgcg ccgccggtgc gccgggcggc
     8461 ggccgccgca ctccggctgc tgccaccgct gcccgcgcac gccgccggtg tggtggaggt
     8521 cgacgttcgg ggccgcggga tacgtcaact ggcggacacg ggacggcagt tccgcatggc
     8581 cacaggtgtg gccgagcggg acgggcgact ggtcttgggc agcctggtgg aggacgcgat
     8641 cgcctggtgc gacatccggt agacggccgg agcggcccgc tcccgcccac gcccgtcctc
     8701 gcgggggcgg gtcggccgga caggaaggaa ccgtggcgat gagcggatcg gccggtcagg
     8761 aggggaccgt ggcggtgggc ggcgtccacc ggtgggtgcg ggccactgct ccggcgacgc
     8821 cgaagtcctt cttgagctgc tccgggatgg cgtagtgcat gacgcggccc cgggtgagcg
     8881 aggacagttc gaggacggcg gtgaggtggc cgatgcggtc gagcgcccag gctccgagcg
     8941 gggagcggtc ctcgatcgtc tccagtacgc cgaggaggtg cggcaccgcc ttgacgacgg
     9001 tgtcccagcg ggtgcgcggc acctggagcc agtccgcgca ggtgtcgccg acgaggtggc
     9061 ggatgagcgc ggagacgatc gggtcgaaga aggtgccggg cacgatctcc tcgtagaggt
     9121 cgatgagctg ccgggtcagc tcggcgccct cgtcggaggg tgccatgtgc cgcaccatgt
     9181 aaagatcgag gaactcgcgt gcttcctcaa gcgacttggg gacggcctcc tggtcgacgc
     9241 cgagcatcgc gccgaccacg cgccaggcgt agtagtacgc ctcggctcct tccgtggaca
     9301 tgtggatgcc gaggcggtgc agactgtcca ggacgagcag ggagaagaac atctgcccgc
     9361 cgatcatgtc ctcctggcag atcggggtgc cgagcgtccc ggtgtcccaa cggttctccc
     9421 gcttgaggtg gtggcggatg gaggcgtgca ggaggcggac cttctgcgcg gcggggatga
     9481 accggctgcc cgcctcgaag gcgtcgggct gcatcaggta gacggtgaac tgcccggtct
     9541 ccgccatccg cttggagggg tacttgagcc cgtgcgtggc cgagaggagt ttcgccacgt
     9601 gcgggacgac gtaacacgcg ggcatggagg cgaaggacag ggcggtggag atgtgcacgt
     9661 tgttgtcgat gaagaacagg cgggcgcgct ccatctcgcc ccagtccacc cagggcggcg
     9721 gcgcgctggt gacctggagg tactcccgcg ccacatcggg cagcccgtcc ggcaagggcg
     9781 cgccgacggt ggagacgtac cgcatcaggg tgttgaactt gcccacctcg ccccgctcga
     9841 agagcgttgc gacggtggcg tcggcgagtt cgtccccggc ctgccgcagg gcgtccatcg
     9901 atgcctcggt gtaggtcatg acaggtctcc tcgtccttca cggtcgtcga cggcaggcgc
     9961 ggtccgccgc cgggcagcac acaggaaccc gcggtgcggg tcaggacaga cggatcgcgg
    10021 cggaccgtgc cagcgcggtg agggcggcag tggcgggcgc gggggcgttc agcgtcccca
    10081 gcacggtgag ggcctcgtcg acccgctcgc cgatcatcag ctcgatgcgt tcgggggcct
    10141 tcaggctgcg catcaccgcg cggacctcgg cgagaccttc ctcgtccaga tcacggcggc
    10201 cgagaagcga gccgagccgc tcccgttcac ccgcgtcggc caggcgccac gtctcggcca
    10261 gcagggcggt gggccggtgg ccgcgcacgt cgtcggagtt ggccttgccc gtgcgttccg
    10321 ggtcgccgaa caggcccagc agatcgtcgc gcagctggaa ggcctccccc agcggaagcc
    10381 cgtacgcgga gtagccctgc cgcagccgct cgccggcccc ggcaagggcc gcgccgatca
    10441 ataggggctg ttcgacggtg tatttggcgg tcttgtaccg gatcaccttc agcgaggcag
    10501 agatgtccgg aggggccccg gtgcgcagga tctccaggca ctcgcccgcg atcagttccc
    10561 gggcgagcag cgaccacagg gggcgggccc gcgccagata ggcggcgggc agtccgctgg
    10621 tggcgaagag ctgcccggcg agtgacatca gcaggtcccc caccagcatc gcgagtgatc
    10681 tggcggccgc gacggcacgc ggccgacggc gtacggcacc gcgtaacgcg atgtgcgccg
    10741 agggccggcc gtgccgcaat gggctgtcgt cgatgaggtc gtcgtgcacc accgccgcgg
    10801 catgcaccag ctccatcgag gccgccgccc gcaccagcgc gtcgctgtcc ggctgcccca
    10861 ccgcgcgcca gccccagtag cagaacgccg accgcagccg tttgccgtcg gcgaccgccg
    10921 cctccaactg gccggccacc gggccgagca gggggtcgat gtccgacagc tcgtcggctt
    10981 ccgcggccac gaaatgccgc agcacctcgt cgacgcgggc cttgaaggcg gccggctccc
    11041 accggtcaga catcgccggc ccgccgagcc agggcgtgcc gggcgagcac ctccaggtgg
    11101 gccggcggcg cggcggcgta ccggtccacc accagacggc cgcgcgccag cagatcgtgc
    11161 tcggcgtccg ccgccatctc agcggtgtcc gagcggcgca gcagtccccg cacgaccccg
    11221 agggccgagc ccagggtgct caccgccaag tcggcgattc ccgccgccaa gaggacggcc
    11281 tgctcgtcca ggccctcacg tcgtcccgct tcccctgtca tggcttttcc cctcaccccg
    11341 cggcacagcc ggacggcgtc cgtcatatgc catgcgttcc agaatcgcgg cgcccgccgg
    11401 tcctctgccg gggaactcac gatcgggtga gggcatcgct cgtccgtgcg ggccgctcgc
    11461 catggcgagc ggcggaggga gccggcaccc gggggccgtg cgggcggtcg cccgaccggg
    11521 cgccgttcgg tcccggtagt ttggcgggcc acccgatcgg gatacccgtg gcacgtgatc
    11581 ccctcccacg tcgatgacac gctgccggag ctggcgccct tcctgcacga ggtgcgggcc
    11641 caccggcccg acgacggcac ctggtgcgag gcatggacgg tacgggacgt gctggcccac
    11701 cagacgggca acgccgagga actggcccgg gtcctcgaag cattcctcgc gggctccccg
    11761 gtggaaacgc gcggcttcga ccgggaggag ccctatcggc ggatgtccga ccccgatctc
    11821 tgggcggctt tcatgagtcg ctgcgaacac ctcgccgaga tcgccgcggc cgccgagcgc
    11881 gatctgtcgc ccgacaccga gatcatgtgg accggccggt cggtgaacgt gccgttcttc
    11941 gccgaacaca tgcgggagga gctgatcctg caccgctggg acctgagcgg tgacgaccgg
    12001 acggcggtgc aggcgctcgc cgagccctgg atgacggagc acagtgtggt gtccgtcggc
    12061 cgtcccctgc tggtcagagg cagcaccggc ctcgatctgg gacccgacgg gaggatcgag
    12121 ggccgtctgc gctgccccgg aaccgacgac gtcgtcgtga cggccgacgc cgggggcacc
    12181 ggcattcgtc tcgccgcccc cgaaggcccc gccaccatcg agagcgacgc ggccacccgg
    12241 gtcctgctgc tgtgggggcg ccgcccggcg gacccgtcac gctggagcag ctcggcaggg
    12301 ccggactccc tgcgcagggt ccggaccctg ctgagcggct actgagccgc ggtcagccga
    12361 agtcggcggc gggctcggtg gcgtaccggc tcatcgtccg gatcgtcgcc tcgggggtca
    12421 tcgcccggcc accggcgacc atgtcctgga ggtcccggaa gtactggacg tacaggtccg
    12481 gggtgaacgt gctgagcatg acggccggtt ggtcggtcac gttggcgaac gtgtgcgggg
    12541 ctcccggcgg gaccatcacg agggtgcccg cggtggcgtc gtagtcgcgg tcccggacgg
    12601 tgaaccgcac ggtgccggag atgacgtaga agccctcgtc gtgctgggcg tgacggtgct
    12661 gcggcgggcc cggggtgtgc ggggcgagca cggactcggc gattcccagc cggtgcccgg
    12721 tgttgctccc gtcttcgagg aggcgcagtc gcgtggtgcc cagcacgatc atctcgccgt
    12781 catccgggcc gaccaccgac actgcgggct cggctgcttc ggcggactcg gcgggctcgg
    12841 cgggctcggc gggcagttcg ctgttcttcg gttcgaaggt catggctcga ttgcactgcg
    12901 cggcccgacg actgtccaag accagtcgcg cagggccgat accggacggg tatcgtcgca
    12961 gcatggagct acgtacgctg cgctatttcg tagcggtcgc cgaggaactc catttcggcc
    13021 gggccgctcg gcgtctgcac atgagccagc cgccgctgag ccgggcgatc aagcagttgg
    13081 agaccgacct cggtgcctcg ctgctcgacc gctccccgct cggggtcgcc ctcacaccag
    13141 cgggcgccgt actgctcgac gaggcccgcg ccctgctcct tggggccgac cgggtacgcg
    13201 gacgcgtggc ccgtgcggcc ggtacggcga ccctcaccct cggcatcctg ggtgacagcg
    13261 ccgacccgcg cgtcctgcgg ctggccgacg cgtaccgccg acggcatcca cacgtggaag
    13321 tacgcgtccg cgaggccgac ttgagcgatc cgacctgcgg gctgcacgcg ggaacggtcg
    13381 atgtcgccct gacccgcggg ccgttctacg agaccggcct caccgtccac gaactccgca
    13441 ccgacccggt gggcgcgctc ctgcgcaccg acgacccgct cgccggtcga gtggccctga
    13501 cattggccga cttggcggat cgacgctggt tcgtcttccc cgagggcacc gacccgcggt
    13561 ggcaggcgta ctggaacggc ggggagccgc gcgaggggcc cgtggtgcga gccgtgcagg
    13621 agtgccgtca ggccgtcctg tggaacggca cggtgggcct gacgctcctg gaccacgacc
    13681 ccggggagag cctgacggtg gtgcccctga ccgacatgcc gccgagccgc ctggtgatgg
    13741 cgtggcgcga gagcgccccg gccccgctcg tccactcgct cgcccgtacc gcggcggccg
    13801 tccacggggc aggcgtccga ggggtttccg acctaggggc tgccgtccgc ggggcataac
    13861 cgcacgacaa cccatcagtc ccgtcgcagc ccgggcgcaa cggcagattg cccgaaacgc
    13921 aaccattact tgccgatcta cgcattgccg ccgtggcacc cccttgccaa gactcggact
    13981 cgacgagtcc gcggtccccg gcgcgggggc tgcatcgtcg actcggcgaa ccggacatca
    14041 gcagacttgg ggagagccat gcgcagcagg aatgtactca tatccggagc cagcgtcgcg
    14101 ggactgacgc tggcccactg gttgcagcgg gccggctttc gacccaccgt ggtggagcgg
    14161 gcgcccgggc tgcgcagggg tgggcaggcc atcgacatcc gtggctcggc gttaggcgtc
    14221 gtggagcgga tggggctgct cgacaaggtc cgctccgcca ccaccgacat gcgaggcatg
    14281 tcgttcgtgg acgcccgggg caaggagttg tcccggtcca ccgaggccac gttgaccggc
    14341 ggtctgatcg acaacgacga cgtcgagatc atgcgggacg acctgacgac cttcctgttc
    14401 gacacgagcc gctacggcac ggagtacctg ttcggcgatg tcatcacgtc cgtcgagcag
    14461 aacgaagaca ccgtggaggt ccgcttccgg cacgaacggc cgcggagctt cgatctggtg
    14521 atcggcgcgg acggactgca ctccaatgtg cggcggctgg cattcggccc cgagtcggac
    14581 ttccttcatt tcctgggcag ttacctcgcc atcttcagcg tgcagaacta cctcggcctc
    14641 gaccactggc agacgtacca caccagcgac gacctgctgg tcggcgtgta cagcgtgcgg
    14701 cagaacaccg aggcgcgggc gatgctgctc ttcgactccc cggaactcga ctacgaccac
    14761 cgcgacctcg atcagcagaa gtcgatcctc ggcgagcggt tcgccggcgt cggctgggag
    14821 acgcccaggc tgctggaaag catgagggac gcgccggact tctacttcga ctcgatgagc
    14881 caggtccacc tcgaccactg gtcgaacggc cgcgtcgggc tcgtcgggga cgccggttac
    14941 tgtgcctcgc cccggtcggg ccagggcacc agcatcgcga tggtcggcgc gtacgtgctc
    15001 gccggcgaac tcgcgaacca cccgcacgac caccgctccg ccttcacccg gtaccaggac
    15061 ctgatgcggg actacgtccg cgacaaccag cggctggccc agaaccgggc ggccgacacc
    15121 aacaggaccc cgcccgacga actcgcccgc gcggccaact cgatcaccct gccggactgg
    15181 ccccacctcg gctgaggtgt cggccgggcg gtcggctcag cggccgcccg gcgacagcgg
    15241 catcggtcag ggagccatct cataggtgcc ggacagcgcc tcgacgcggt cccagacccg
    15301 ggccgagcgg gccgcgtcga cgaccgggcg gcggaccgcg cccagcgccc agccctgctg
    15361 ctccgacgtg gcggagtcct tgccgtgcag ttctacggcg tgcgcggaga agtcccgtac
    15421 gaggacggcg aagagctcgt cgagcacgtc ggcgtccagg tccgtcaggc gtgcctgctc
    15481 caggatcagc tggccgtgca cgacaagggc gaacagctgg ccgacggcga ggagcaggtc
    15541 gaggtcgcgg ctctgctcct cgtcgggcgc ggcggtggtc acgaactcgc agagcgcgtc
    15601 ggcctgttcg cggaagcggg cgacgttggg cagctccgcg aaggcgtcgt aggccgtacg
    15661 ccagtcgtgg aagcgcacgg atccaagacc gcgggccgga ccctgctgga agaggaaggc
    15721 gtcgtcggcc gcgtcgaggc gggtcgggac gggcgcgtag tccgccgggt tcagcaggtg
    15781 gttgcgcatg aacttgagga tcagggcgag gttgacgtgg accgtgccct ccagcttggg
    15841 cagaccgcgg atttcgacgg cggcctgggc gaagtagttg tccttctcga agcccttggc
    15901 cgcgatcacg tcccacatca ggtcgatgac cttctcgccc tccgtggtca ccttcatctt
    15961 cgtcatcggg ttgaagagga ggtagcggcg gtcttcgggc cccgcggagc ggaagtagtc
    16021 gacggcccgg tcgctgaaca gcttcatccc tacgaggcgc acgtacgcgt cggtcagctc
    16081 gcgccgcaca tgcgggaagg cggtgacggg gcggccgtac aggatgcggt tctgcgcgtg
    16141 cgtgacggcc tcgtacatcg cgtgctcgca gatgccgatc gaggcggtgc acaggttgaa
    16201 cttgccgacg ttgaccgtgt tgagggcggc gtcgaaggcg gcccggccgg agtgcaggac
    16261 gtcctcggcg cggaccgggt agttctccag acggaactcg ctgacgtact tcgaggagtc
    16321 gacgacgttc ttcacgaggt ggtacgcctc gtggcggctg tcggcggcga agaagacgta
    16381 gccgtcgggg ccttcgacgt cggtgcggcg gccgaagacg gagacgagcc cggcggcgtt
    16441 gccgttgccg atgtagtact tgctgccggt ggcccggaac ccgccgtcgc cgtcgggctc
    16501 caggagcatg tcggtggagt agatgtccgc gccgtgggtc ttctcggaca ggccgaaggc
    16561 gaacacctcg ccctgggcca gcagttcggc cgcgcgggtg cgggcagcgg cgttgtcgct
    16621 ctgccagacc ggtccgaggc cgaggatggt gacctgccag gcgtaccagt agtcgaggcc
    16681 gtagaagccg aagatctcgt tcagggcggc gatccgcgcg gtgtcccagc gcttgtcgcc
    16741 ctcgccgtcc ccggcggcgg aggccggagt gaggaaggtg gcgaagaggc cttccttggc
    16801 ggagaacgcc aggaagtcgg ccagccaggc gcgcgagcgg tagtcctcga tgagccggcg
    16861 cttgccacgc tgctcgaacc agtcgaccgt cgcgcgcagc agcctgcggg tctcggggtc
    16921 gaagtgcttc gggtcgtagg tgtgcgggtt gaacagcagc gcgtcggcca tggccattcg
    16981 cctttccggc tcggggagtt gaggaggggt ggacggaaga ctcggtggaa caaggagcgt
    17041 gggtcggtgg tgggcgggcg ggaaggagcg gcccgggcgg gcgcccgcgc ggatgcgctc
    17101 ggttcagcgc tcggcgccga ggcggtggag ggtggcgatc acgtcgtcga gccaggcgag
    17161 cgtcatccgt tcgtacgcga tgccgccgcg cagcaccacg tgctggagct cgcggcccgg
    17221 gtcgggtgcg ggggcctcgg gtccggtgaa gtcgcgaagc tctccggcga gatagtgcgc
    17281 gaggcggtcg gcgtgtgccc tgcggtgccg ctcgacctcg tggagcaggg cggcggggtc
    17341 gtcgaaggcc gccccacgga tcttcacggc gagctcgtgc cggacgctct cgggttcgat
    17401 cggctcgtgg agccaccgcg agagggcggc ccggccgggg tcggcgacgg agtactcctt
    17461 cttgtccggc cggccggcct gcgccacctc acgggcgtcg atccagccgt cgccctccat
    17521 ccgcttgagg atgcggtaga tctgctggtg ggtggccgcc cagaagtagc cgatggaccg
    17581 gtcgaagcgc cgggccagct cataaccgga gcccggcttc tccaacagcg agacgagaat
    17641 cgcgtgctca agcgccatgc ccgaatcctt ctatgcaact cgttgcatag acaagcggca
    17701 ccggccaggt gagacacggc tcacccggtg cccttgtgcc gggtgccgtt gccacgttgg
    17761 ctgtctcacc cagcgcggcg gcggtccggc gggccgcccc gcggtgcgag ccatgaggag
    17821 cggccatgca catccccggt cccgagctcc ggaccgacgg cggagcgggc gccatcgcgg
    17881 cagcgggcgg cctgcaccac taccagcgac tgctgcacgc cgcctacggt cccgtcgtac
    17941 ggttccagcc gccgggcgcc gagaccgcgg tgtcgatcgc cgatcccgta ctcctggagg
    18001 ccacagccca cctcgacgaa cggccggagc ggctcttcga cttcctgact cccctgttcg
    18061 aggcgggaaa cgtgcaggtg ctgcccgtcg aggagcacac cccctggcgt cgcctgctgt
    18121 tgtcggtcct ggccgggcgc ccctcccacg agcggcactt cgcgcggttc acggagctga
    18181 cgacggcgcc ggcggacagc tggtccgaag gcgcccacgc cggtaccgac cgggaacccg
    18241 tcgctctcca gaaggacctg ggtgatggcc tccgtcgtct cccgggcgcc gtagggcccg
    18301 ggcggagggt gcgcaactcg ccacgatcga ggcgcagttg atcctcgcca ttttgctcaa
    18361 gcgcttccgg ttccggccgg tgccagggca caaggtcgtc ccgatcgagc ggttcgtgct
    18421 gtgggcggcg gacgacatcc gcatgaccgt gcgccgccgg gagtgaccga agcccgctgg
    18481 acctcatgcg ccttgccctg ccccttgccg gatcgcttac gccgtggggg acttcgaagc
    18541 ggcggcttcc aggtcgtcga tgctgcccga catgatcgtc cgtacgtgtt cggtgatgaa
    18601 ttccgccggc cagtcccacc accccaccgc gagcagccgg gcgacatccg gttcgctgta
    18661 gcggctgcgg atgagacggg ccgggtttcc tccgacgatt ccgtagtcag ggacgtcgga
    18721 ggtgaccacg gcgcccgcgg cgatgatcgc gccgtggccg atgcgcacgc cgggcatcac
    18781 ggtggcgccg tgcccgaacc agacgtcatg gccgacgacg gtgtcccccc ggttgggcag
    18841 gccggtgagc agatcgaagt gctcggccca tgagccaccc atggtgggga aggggaacgt
    18901 cgaaggcccg tccatgcggt gattggcccc gttcatgatg aaccgcactc cggtgccgag
    18961 cgcacagaac ctgccgatga tcagcttctc cggcccgtag tggtagagca cgttgcgcgt
    19021 ttcgaacgcg gtcgcgtcgt ccgggtcgtc gtagtaggag tactccccca cctcgatcag
    19081 cggggaggtc acgagcggct tcagcagcac cacccgtggc tgcccgggca tcgggtggag
    19141 gaccgtggag tcggcgggta cgggcatggg ggcgcttcct ttcgtgcggc gtgcggcgtg
    19201 cggcgtccgg cgtccggcgt ccggcgtccg gcgtccggcg tccacgccat gatcgtcgcg
    19261 tcacgctgcc ggggaaagtg ctttctccac ccgccactcc aactaccgcg cgcaggcccg
    19321 gtcttgactg ccgcccgccg tcgcaccgcc gtcggcagcc ggcgcggtgg cggtccaacc
    19381 cgcttccacg aagtcgaagg tcacgtcgat gatccgggcc ccttcgtcga gcgacgtgat
    19441 cgacgggatc accccgagcg ccgttcgggc gaccagccgt gaggcggtgt cgggagcggc
    19501 gagcccgatg tcggcggcga tgacattcgc cagcgcctgc tcgtggcgca gccacatcct
    19561 ggccgagtag tcccgcaggg cgggcgtcgt ctcgatgagc cgatggaagc ggtccaggtc
    19621 cggctcattc ctggccgcgt cgaccattcc gtggaaccag cgacgcaggg cctcgggaat
    19681 ggagacgccc tcggggcgcg tgcgtacgac gtcgagcaga gcctcctcct gctcgtcgtc
    19741 ctggtcgaag accaaggcct ccttcgaggg gaagtgggcg aacagcgtgg cggtcgccac
    19801 gtccgcctcc tgcgccacct cgcgtacgcc gacagcgtcg tatccgcgct ccagaaacag
    19861 ccgcagggcg gtctccgcaa tcgtcctgcg cgtctgggcc tttttgcgct cacgccgccc
    19921 gagctgctcg tttgtcgccc catgtgccgc catgagacaa ccatagcagt tatgaagtcg
    19981 gaacagttcc aattacagta ctgttccgaa aggcgctgca ccgcggcgtt ccgaccgccg
    20041 actggaggaa gccgtgaaca cgtcacccat cacccccggg gacccaccgg cgaaggagcc
    20101 gtccggacgt cggcgggcat ggctcgtcat cgtgtgtctg accctgctca actcgatcgg
    20161 gataaccgtg gtctttccgg tccttccgtt cgtcacgctc gaatacgtcg acgcggggtc
    20221 actcgccctg tgggtcggcg tcctcgaatc ggtcaacgcc ctgtgcgcgt tcctcgtcgc
    20281 gccgctgctc ggcggcctgt ccgaccgggt gggccgtcgc ccggtcctga tcatcagcgc
    20341 cttcggcgcc gcggccggtt atgcgatgtt cggtgtcggc ggcgccttgt gggtcctggt
    20401 cgcggcccgg gcggtccagg ggctcacggc cggagacatg ccggcgatat tcgcctacgt
    20461 cgcggacatc accccggcga aggaccgcac caagcggtac gggctgctcg gcgcgctgac
    20521 cggcgtcggc atgatggtcg ggcccgcgat cggcggcctt ctcgccaagg tgagcgtcga
    20581 tgccccggtc tacgcgaccg cggtgaccgc cgtcctggtg ggtggggtct ccatcttcgc
    20641 gctccccgag agcctcgccc cgcagcaccg gacggcgcgg ctgaagctcg aagacctcca
    20701 cccgttcaag gtcctcaagg acgccttcgc ccgcaccgaa ctgcgcgccc tgctcatggg
    20761 attcaccctg gtcagcatcc cgttctactt ctacgccaac aacctgtccg tgctcggcag
    20821 ggacaccgtg ggatgggggc ccacccaggt cggcctgttg ctctcggtcg ccggaattct
    20881 cgacatagcg atccagggcg gactgctcgc cctgctcctg ccccgcatcg gggagcgcgg
    20941 tgtcgtcgtc tcggggatcg tcgcccaggc agtgggctgc ggcggcatgg ccctggtcgc
    21001 cctctccctc gacgcacccc aactcctggc cgtggcagcc ctgttgttcg cat
//
