LOCUS       NZ_CP070373            21257 bp    DNA     linear   CON 18-MAY-2024
DEFINITION  Streptomyces sp. MBT27 chromosome, complete genome.
ACCESSION   NZ_CP070373
VERSION     NZ_CP070373.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MBT27
  ORGANISM  Streptomyces sp. MBT27
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S.S., Hankemeier,T. and van
            Wezel,G.P.
  TITLE     Discovery of novel glycerolated quinazolinones from Streptomyces sp.
            MBT27
  JOURNAL   J Ind Microbiol Biotechnol 46 (3-4), 483-492 (2019)
   PUBMED   30729343
REFERENCE   2  (bases 1 to 8400223)
  AUTHORS   Machushynets,N., Elsayed,S., Carrion,V.J., Siegler,M.A., de la
            Cruz,M., Genilloud,O., Hankemeier,T. and van Wezel,G.P.
  TITLE     New from old: conjugation of actinomycin X2 and anthranilamide to
            form the novel actinomycin L in Streptomyces sp. MBT27
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8400223)
  AUTHORS   Du,C., Machushynets,N. and van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2021) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   4  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S., Hankemeier,T. and Van
            Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2020) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 1.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011751035.2-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 03:23:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,575
            CDSs (total)                      :: 7,482
            Genes (coding)                    :: 7,345
            CDSs (with protein)               :: 7,345
            Genes (RNA)                       :: 93
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 39 of 137
            Pseudo Genes (incomplete)         :: 114 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:14:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4128142
            Orig. end                         :: 4149399
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MBT, Institute of Biology
            Leiden, Leiden University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21257
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="indole"
                     /region_number="18"
                     /rules="(indsynth or dmat or indole_PTase)"
                     /tool="antismash"
     cand_cluster    1..21257
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(indsynth or dmat or indole_PTase)"
                     /kind="single"
                     /product="indole"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21257
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:11257](+)"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11257
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="1"
     gene            complement(414..971)
                     /locus_tag="GWQ54_RS18150"
     CDS             complement(414..971)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133663.1"
                     /locus_tag="GWQ54_RS18150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX hydrolase"
                     /protein_id="WP_167150989.1"
                     /transl_table=11
                     /translation="MIEGWRTLSSTVQFTGVRLVVRRDVVRRTDQSQGTYEYTESTDGV
                     RVVALDGQGRIALVEENVYVCGQRLLMCPGGGCEPGEDPSAAARRELGEEAGLHASEVE
                     LLTTMWRMPAGARTREHLYLARGLRIGEHHRDASEADMELRWIALDEALAMCGDGRITE
                     AGTLAAILLTAHRIAPRPPSRQ"
     gene            complement(1050..3050)
                     /locus_tag="GWQ54_RS18155"
     CDS             complement(1050..3050)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743401.1"
                     /locus_tag="GWQ54_RS18155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_167150987.1"
                     /transl_table=11
                     /translation="MSLWRARMTEPAVSAAEEELFGGALRYDAAWSEHERSRVEVTLLS
                     ALRSMPRLVGATVKRAWRADRSGLVVLGIAEVGQGIAAAIGLLIVNSVMHALLGGGSAP
                     ERLHQALPAVVAGACAAVIRSLFAGLSTSRAGRLEPLVERIAQVQYLKAAAEVELEAIE
                     DPEFKRLMDVAQFGVDASRRMISACIAALNGVISLVSAAGVLSVLDVRLLPMLLLIAAP
                     RAWGAMRVAQERYVSRLTWVEHLRASRLLGNLLTERAAAQEVRVHQVGPFLLTKYEQMA
                     TTAESEQRRLATRKAMIELLAASMSGVATALTYAAVYGMITAGWMSLAVAGTAILAIRS
                     GSASLGSLVMNLNQLNEESLYVRDHEAFVTGAVTRTIPHGGEPLASRLGAVELEKVSYR
                     YPDREHAALHEVSLRQEMGRVVAVVGENGSGKSTLMKVLSGLVSPAGGTVRFGRQHLAD
                     VDRRQVFARVSVLTQDFQRWPLTAAMNLRIGRPEHPHTDDALMRSLRHADADRLWEKFP
                     AGWQSLLGRMFRGSADLSGGEWQRIGLARTHWRTITQDADSVLIVDEPTSALDPETEIE
                     AFRRVRALASPHRVVVLVTHRMSGVRHADMIHVLHQGHLVESGSHQELMARPDGRYRAM
                     FLAQAAEYASIADDHVPGPRVGDPSPRGQSS"
     gene            3905..4873
                     /locus_tag="GWQ54_RS18160"
     CDS             3905..4873
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103102.1"
                     /locus_tag="GWQ54_RS18160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_167155805.1"
                     /transl_table=11
                     /translation="MSVEAVFPELAPYRARTTRLHPRSGRPGMHDSHVGGPMLWPDGEQ
                     WPLCHEPHQHETEGYAPEEIRSARAAGVWPPSRPWLPAGIHGLDDPVPFVGLAQFFRRD
                     IPNLLCGPDGADLVQLFRCPFTHGPDLERRYRLEWRRARDTERAQRFLAAPPEVPLLRW
                     EHELPEPCVLHPEEVDTYPWAEDDTLPGGLIARIDAWDDAQAAEHGPDAPSYQYDLSIP
                     PGWRVGGHPSWASTGPMAIDCASCAAPMRLLLTAEGNEMDAGSHSWAPLEDRDPSMRGK
                     ASIRGGQPVAQPTRLRFGRDRDLHIFTCPANPGHPPQSVLA"
     misc_feature    complement(5053..5068)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.09"
                     /tool="antismash"
     misc_feature    complement(5528..5542)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.17"
                     /tool="antismash"
     gene            5745..6944
                     /locus_tag="GWQ54_RS18165"
     CDS             5745..6944
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177156.1"
                     /locus_tag="GWQ54_RS18165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_229861651.1"
                     /transl_table=11
                     /translation="MTNFWQDPTVWALVVGVAVAATVITRQRKTIKALSRHKQGLHGEL
                     TASQGRATDLQSTVTELRNGYDEVVRQAKEEAEEATNTVLKSAMRTLQGLAAEQQRAIS
                     GLQKKYGDSAVLRDMLDIDHMNSQFNRRAQSIAVLCGGWLGRQREAASVHDVIRGAQGR
                     IRHYQRIEIASRVDFAVTSRAVEPVALAVAELLDNASSYSEPSTMVEVEVRTVPRGICI
                     VIDDAGVGMSEEERTNATALLSSGSAVSVSELGNPPAFGFAVIGALCARFGFTVTIDST
                     SPYGGVRAVVMVPKELLTEMPEPKAPTPAREVNAVAASRGSGAAATEGGLPRRRNKRGM
                     ALVPDGGGGSDEAPPARSAEARVAAIGAFQRGTLSGRGARVPAAEGDTAADTHEGLDAP
                     "
     gene            6941..7339
                     /locus_tag="GWQ54_RS18170"
     CDS             6941..7339
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552722.1"
                     /locus_tag="GWQ54_RS18170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="WP_167150985.1"
                     /transl_table=11
                     /translation="MSDNLSWMLEDALQIPHALHAVLISADGLQMSRTAEIDRGDADKV
                     AAAVSGLQSLSRAVGFFCGEQGTNWRQTLIEFDGGWVFLNAAGGGSYLAVAASVEVDMQ
                     DITYRMQQLVSRLGKAMSTGLRGDTVTS"
     gene            7336..7725
                     /locus_tag="GWQ54_RS18175"
     CDS             7336..7725
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552723.1"
                     /locus_tag="GWQ54_RS18175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF742 domain-containing protein"
                     /protein_id="WP_167150983.1"
                     /transl_table=11
                     /translation="MTEPGRPEPEFVSEAGELVRPYVITRGRDLPDEDEFALITLVTTA
                     TDEQQRPQRLSPEEQQILEMCSSGYLSVAEIAAYSQLPLGVVKVLLSSLAEGGYLVARA
                     PVPSARPTNKALLKEVLDGLKALTV"
     misc_feature    7387..7389
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            7700..8305
                     /locus_tag="GWQ54_RS18180"
     CDS             7700..8305
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005331.1"
                     /locus_tag="GWQ54_RS18180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP/GTP-binding protein"
                     /protein_id="WP_167150982.1"
                     /transl_table=11
                     /translation="MASRLSLSEDAYVPSGAQQTAVKILVVGHFAVGKTTLIGSLSEIT
                     PLSTEERMTTLSERVDDLKGVQGKTTTTVALDFGRLTLSERIVLYLFGSPGQQRFVSLW
                     DDTARGALGALVLVDPERLADSFDIMDLVESYGLNFAVAINHFDGSTVHTPEEVREALD
                     LLPDTPVVTIDARDEKSSVDALITLVRYLYERADLEHA"
     gene            8302..9648
                     /locus_tag="GWQ54_RS18185"
     CDS             8302..9648
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552725.1"
                     /locus_tag="GWQ54_RS18185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_167150981.1"
                     /transl_table=11
                     /translation="MTTPSVPPPGCPAHGSGQRIPLDSAEFAANPHAYYRYMRELGPTA
                     PVTIAPGVEATLVTDYNAALELLQDSTTFRKDSRRWRALAEGCIRPDSPVLPLLAYRPN
                     AMFTDGAEHVRLRQAITDSFARVSNKRLSRIVSQAAQFLINQFSARGSADLLRDYAQQL
                     PLYVFNELFGCPAEIGDRVLFGISGMFDGVNAEAASQVLLGAVGELVALKRARPGEDVT
                     SYLMQHPAKLNDEELAHTLVLLLGAGGEPEGNLIGNAFYTMLTNEEFARLGQFDKAVDD
                     TLWRNAPMSNYAPHYPVVDTDVAGDKVRAGDLVLVSFGAANTALADRGADTRGHLAWSA
                     GPHACPSKEPARLIALTGLETLFNALPDVELAVPADSLAWRPGPFNRALASLPVRFSAV
                     TPRAAAAPEPTAVPRYQAPPVAAGPAPAAPQPNGKAGRWSSFMKWLTGQ"
     gene            10001..11257
                     /locus_tag="GWQ54_RS18190"
     CDS             10001..11257
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /GO_process="GO:0009820 - alkaloid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) indole:
                     indole_PTase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552726.1"
                     /locus_tag="GWQ54_RS18190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tryptophan dimethylallyltransferase family
                     protein"
                     /protein_id="WP_167150980.1"
                     /sec_met_domain="indole_PTase (E-value: 2.6e-121, bitscore:
                     403.7, seeds: 22, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSRPGPGIPATGPGETTLGDHVLGQLRRLGATVGLSERDTELYG
                     QVLIESLAGAAHRPLSLPPASSGFLSDDHTPVEFSLAATPDTAPALRVLVEPGCAHSDM
                     ADSGRAGLAVIRAMGARWGFDTGQLDSLADLFLPAAAQGPLALWYALDLRAGGVPGVKV
                     YLNPSASGPENAARTVREALRRLGYGRAFEQLPQAAGYPFLALDLGAWTSPRVKVYVSH
                     LGMSADDACGLSRTSGAAAADIRYFFHAASGSLAPSESGADDTLRLVRRPALTCHSFTE
                     GDSEPSGFTLHIPVRDYVRDDEEALARATALLTRAGMDPTVLNRSLVALTQRSLSDGVG
                     LIAYLALVYERNRPPRLTAYLSSEAYRVRPPQEQYPRGTWMPAQRTRSVLDRGHQAVAH
                     VSPYTAQKEVTWSPTASRS"
     gene            11230..12603
                     /locus_tag="GWQ54_RS18195"
     CDS             11230..12603
                     /NRPS_PKS="Domain: Beta_elim_lyase (41-419). E-value:
                     5.2e-75. Score: 244.3. Matches aSDomain:
                     nrpspksdomains_GWQ54_RS18195_Beta_elim_lyase.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552727.1"
                     /locus_tag="GWQ54_RS18195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tryptophanase"
                     /protein_id="WP_167150979.1"
                     /transl_table=11
                     /translation="MEPYRIKVVEPLAFTTREEREAALKRVAYNPFDLRADEVTIDLLS
                     DSGTGAISADQLAAGMQGDESYAGSRSFYRWHEVVSELTGYPHILPAHQGRAAERVLFS
                     SLLRPGTSVLSNTHFDTTRANVELTGCTAYDLPCAEAKDLDSDFPFKGNIDLVALEQAL
                     ENADRTPVGAVLMTITNNGGGGQPVSMDNLHRTAELCRRHNVPMILDAARFAENAWLVT
                     QREPGYADRTPRQVAEEAFRLADGCVMSAKKDGIVHIGGFIGLKDPELAERCGGLLIAT
                     EGFTTYGGLSGRDLDMMARGLLEVTEPAYLAERADIAAYLAARIREAGVDILEPAGLHA
                     LYVNAGRLLPHIPPHQYPGTALVCELYLRGGIRSAELGSLYLGEEDEHGNPVKTAPYEL
                     VRLALPRRVYTRSHYDHVAATLADIAKNPESVHGYRVTGQSPILRHFSIKLEPVKTGD"
     aSDomain        11353..12486
                     /aSDomain="Beta_elim_lyase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GWQ54_RS18195_Beta_elim_lyase.1"
                     /evalue="5.20E-75"
                     /label="GWQ54_RS18195_Beta_elim_lyase.1"
                     /locus_tag="GWQ54_RS18195"
                     /protein_end="419"
                     /protein_start="41"
                     /score="244.3"
                     /tool="antismash"
                     /translation="DLLSDSGTGAISADQLAAGMQGDESYAGSRSFYRWHEVVSELTGY
                     PHILPAHQGRAAERVLFSSLLRPGTSVLSNTHFDTTRANVELTGCTAYDLPCAEAKDLD
                     SDFPFKGNIDLVALEQALENADRTPVGAVLMTITNNGGGGQPVSMDNLHRTAELCRRHN
                     VPMILDAARFAENAWLVTQREPGYADRTPRQVAEEAFRLADGCVMSAKKDGIVHIGGFI
                     GLKDPELAERCGGLLIATEGFTTYGGLSGRDLDMMARGLLEVTEPAYLAERADIAAYLA
                     ARIREAGVDILEPAGLHALYVNAGRLLPHIPPHQYPGTALVCELYLRGGIRSAELGSLY
                     LGEEDEHGNPVKTAPYELVRLALPRRVYTRSHYDHVAA"
     misc_feature    12722..12738
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 18.31"
                     /tool="antismash"
     gene            13040..14542
                     /locus_tag="GWQ54_RS18200"
     CDS             13040..14542
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138227.1"
                     /locus_tag="GWQ54_RS18200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metallophosphoesterase"
                     /protein_id="WP_167150978.1"
                     /transl_table=11
                     /translation="MPHSSRDTPEGAGWGNSQLGTYKSLMPARVEKLSWLNPGTLWAAR
                     NGVLASWFGDPTGRTRSRWVEQAVAAGAPPDAVVRRTDADHFSFMVIGDTGEGDDPQYA
                     VIPGFLKVSQGTDFAVLASDVIYPVGSTDDYGKKFFRPYRNYPAPIYAIPGNHDWYEGL
                     QGFMRVFCEAPPLASEARPRPLTRAWLRSLLWHAPGSTDEHRLAEARALRSEPSQQAVQ
                     PGPYWAVDAGPIRIIGIDTGLLGTLDAEQGRWLREVSRGDRPKILVTGSPLYVDGEHHP
                     CAIEGGGTVDAIVRDPEHHYVAAIGGDIHNYQRYPVRVDGGRTIQYVVAGGGGAFMHAT
                     HTVPRVSVAGVTEHDFRCYPLRGDSLAFYSRLYGHRLRMPRFFTLTEAEALAVVAERLG
                     VPPIRAQGEPVRVTRRIRLVASLLGAGRRPDRKARLRLPVRKLYTQLFSPGSATYSPPF
                     FKCFLRLDVTPAAIRLRCHSATGTLDHEIDPPVEDEVTIPLS"
     gene            complement(15035..16060)
                     /locus_tag="GWQ54_RS18205"
     CDS             complement(15035..16060)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006349740.1"
                     /locus_tag="GWQ54_RS18205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="C40 family peptidase"
                     /protein_id="WP_205530672.1"
                     /transl_table=11
                     /translation="MGSHRRPKPASRTRVTVFSATAAAVVALTSQAAHADPKPTKDEVK
                     SKVDKLYDEAEQATEAANGAKEKQGRLEKQIGQIQDQVARGQQELNTLRQGLGSLASAQ
                     YRSGGIDPSLALFLSSNPDDFLDKASSLDQLSATQAEALTKIQGKQRTLAQQRKEAQDK
                     LKDLADTSKVLDDKKREVQGKLGDAQKLLNSLTAQEKAALATEESRANRSTERPNLGKG
                     VPASQVGAAALAAAQTQLGKPYGWSKTGMAAFDCSGLTGYAYRQAGVNLPRTSQEQANA
                     GTRIGRSELKPGDLVLFFGDLHHIGLYAGNNMVLHAPRTGTVVRYESMDNMPFQFGVRI
                     G"
     misc_feature    16960..16973
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            complement(17067..17768)
                     /locus_tag="GWQ54_RS18210"
     CDS             complement(17067..17768)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GWQ54_RS18210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167150976.1"
                     /transl_table=11
                     /translation="MCGRYEASPHDEPPGLGPFSTEGIALLDHHPDTPPTKPEREKGSG
                     KEQTTVSLGSSEIFSQVVKGHAQNGAVVGAFAGLAAGISNLGQSIFEFPNATRNHVTAG
                     VCGFVIMLIFWVLKGIFQADSAKSDIITVNDDGLTITRNDVKQTFTIKWSAVDRIGLGI
                     LGVRVWFREGSEPTRDWLDKHQIRKQLPDKSFIVYRDDHEPHIPVERLDEALRTFAGPC
                     YKDATNPPGRR"
     gene            <17884..18315
                     /locus_tag="GWQ54_RS18215"
                     /pseudo=""
     CDS             <17884..18315
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025447.4"
                     /locus_tag="GWQ54_RS18215"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF4262 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="VVMVPEDEVGPGFAYTIALAHNHGGPELAMFGLDVRAMQRMLNTP
                     GKRSAAGEVLEDGQSHPDVVDGHRVALRRVDRGWYRTFFGQAVGFYRRPPLPVVQIAWP
                     DAEGCFHWDEQAEDRHRELQPQLWLPPSDHPVGIWTTEL"
     misc_feature    18250..18252
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            18426..18812
                     /locus_tag="GWQ54_RS18220"
     CDS             18426..18812
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016823021.1"
                     /locus_tag="GWQ54_RS18220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6228 family protein"
                     /protein_id="WP_167150974.1"
                     /transl_table=11
                     /translation="MSVIEVEPGQVELVVQGAGASACSVRLSGWSREDEYAASFAVEAI
                     NEGVRARLATATVTVWANMDGFFDSLARDFRGWEGERVWTSSHLEVTVAFGSGGHVYLD
                     WTLRSGFFPGNWRCTVTTVIEADE"
     gene            complement(19650..20594)
                     /locus_tag="GWQ54_RS18225"
     CDS             complement(19650..20594)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GWQ54_RS18225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_205530673.1"
                     /transl_table=11
                     /translation="MQQLVVVSPPGSGIELLHPAITALGYTPRGTQNAPAESPAPARPG
                     EVFPLLEAAYGQREAVQLLQDHHHDPNTTNQLQNAYHQAVLALWRVWWKRLGQPVTAHT
                     PLDTAVEEHLARQDDAQLISMLPGRNAWYTTELDLTRVDGGLLRHWTATGQPAIFYLHR
                     DPGDRITNLIQHLCREDGRVGTIPAYLVYRDILMALPTMDARLTHALTDPQFPGHTSAR
                     QTAWLHHHPAVVTLTAEDLAGQGYGGTDQDRLAALSRLAGALAQPPSAAATLPDPPPGR
                     PDLTTGVFQQHSHTHHRQLLDTLYTSADRLPAP"
ORIGIN
        1 aggggaccac ggcctgggac gggattccgg tctcctcgtg cagctcgcgc agagccgcct
       61 gtgccaggga ctcgtccccg ggttcggcgt gtccccctgg agccagtacc ttcccgctcg
      121 ccaggtgcag cacgtgcagg acgcggccga gggggtcgac gacgatgacg ctgcaggtga
      181 cgtgccccga gaaggtggag cggctcgcga tgtcgtcgcc ggtgcggtcg agggcttcca
      241 ggaggccgtg caggtgaggg cgctcgtgag ggtggcgggc gagataggac tcgacggtgg
      301 tgcggatgtg gtcgtgcgac agcggcatgg ttgcacctcg aaggcaggct gtgaggaacg
      361 ggctgtgggg cctgggctga tgagccgttc gccgttcgtg aggcggctgg ggctcactgc
      421 cgtgatggtg gccggggggc gatgcggtgc gcggtcagca ggatcgccgc gagagtgccg
      481 gcttcggtga tccgcccgtc gccgcacatc gcgagcgcct cgtccaaggc gatccagcgg
      541 agctccatgt cggcctcgct cgcgtcgcgg tggtgctccc cgatgcgcag gccgcgggcg
      601 aggtacaggt gctcacgggt gcgggcgccg gcgggcatcc gccacatggt cgtgagcagc
      661 tcgacctcgg aggcgtggag accggcctcc tcgccgagtt cccggcgcgc ggccgccgac
      721 gggtcttcgc ctggctcgca tccgccaccg gggcacatca acagccgctg accgcatacg
      781 tagacgtttt cttccacgag ggcgatccgg ccctggccgt cgagggctac gacgcgcacg
      841 ccgtcggtcg actcggtgta ctcgtaggtt ccctggctct ggtccgtgcg ccggaccacg
      901 tcgcggcgga ccaccagacg aactccggtg aactggacgg tcgatgagag tgttcgccag
      961 ccttcgatca cgttgagttc tccttgggtg aggacggctt tccggcctgc cttcgtcggc
     1021 gacgccgctg aggggggccg gaggtggtgc tacgaggact ggccgcgggg agacggatca
     1081 cccacgcgcg ggccgggcac gtggtcgtcc gcgatgctcg cgtattcggc ggcctgggcc
     1141 agaaacatgg cccggtagcg gccgtcgggg cgggccatca gctcctggtg actgccggat
     1201 tcgaccaggt gtccctgatg aaggacgtga atcatgtcgg cgtggcgtac cccggacatg
     1261 cgatgggtga cgaggacgac gacgcgatgg ggtgaggcga gggctcgtac gcgccggaac
     1321 gcttcgatct cggtctccgg gtcgagggcg gaggtcggct cgtccacgat caggacgctg
     1381 tcggcgtcct gcgtgatcgt ccgccagtgg gtgcgggcca gaccgatgcg ctgccactcg
     1441 cctcccgaga gatctgcgga accccggaac atccgcccga gcagggactg ccagccggcg
     1501 gggaacttct cccacagccg gtcggcgtcg gcgtgccgca gcgaccgcat cagggcgtcg
     1561 tcggtgtgcg ggtgctcggg ccgcccaatg cgcagattca tcgccgcagt cagcggccag
     1621 cgctgaaagt cctgagtgag gacggagacc ctggcgaaga cctggcgccg gtcgacgtcg
     1681 gccaggtgct gccggccgaa gcggactgtg ccaccagcgg gactgacgag ccccgaaagg
     1741 accttcatca gggtggactt gccggatccg ttttcgccga cgaccgcgac cacccggccc
     1801 atctcctgtc gtagcgacac ctcgtgcagg gcggcatgtt cgcggtcggg ataccggtag
     1861 gacaccttct ccagctccac cgcacccagc cggctcgcca gtggctcacc gccgtggggg
     1921 atcgtgcgtg tgacggcgcc cgtcacgaaa gcctcgtggt cgcggacgta aagagactcc
     1981 tcgttcagct ggttcaggtt catgacgagg gagccgagcg acgccgatcc ggagcggatg
     2041 gccaggatcg cggtgcccgc gactgcgagg ctcatccacc ccgccgtgat catcccgtac
     2101 accgccgcgt aggtcagagc cgtcgcaacc ccggacatac tggccgcgag cagctcgatc
     2161 atggccttgc gggtcgccag acgccgctgc tccgactcgg ccgtcgtcgc catctgttcg
     2221 tacttggtca ggaggaacgg cccgacctga tgcacccgga cctcctgcgc cgccgcccgc
     2281 tcggtcagca agttgccgag cagccgcgat gcgcggaggt gctcgaccca ggtcagccgg
     2341 gagacgtagc gctcctgcgc gacccgcatc gccccccaag cacgcggggc ggcgatcagc
     2401 aggagcatcg gcagcagccg cacgtccagg acgctcagca cgcccgccgc cgacaccagt
     2461 gagatcactc cgttcagcgc cgcgatgcac gcggagatca tcctccggga ggcatccacc
     2521 ccgaactggg ccacatccat gagccgtttg aattcggggt cctcgatggc ctccagctcc
     2581 acctcggccg cggccttgag gtactggacc tgggcgatcc gctccaccag cggctccaga
     2641 cgccccgccc gcgacgtgga cagccccgcg aacagcgacc ggatcaccgc cgcacaggct
     2701 ccggcgacga ccgccggcag ggcctgatgc agccgctcgg gcgcgcttcc gccgcccagc
     2761 agggcgtgca tgaccgaatt cacgatcagc agaccgatcg cggccgcgat gccctggccg
     2821 acctccgcga tgcccagcac caccagaccc gaccggtccg cccgccacgc ccgcttcacc
     2881 gtcgccccga ccagacgcgg catcgaccgc aacgcggaca gcagcgtcac ctcgacgcgc
     2941 gaccgctcgt gctccgacca cgccgcgtcg tagcgcaagg ctccgccgaa cagctcctcc
     3001 tccgccgccg acacggccgg ctccgtcatc cgcgcacgcc acaaactcat cgacgactcc
     3061 tggagcccgc gcggccggaa accgtcgcac cgggcatcga agaggcagcc tgaccagggt
     3121 gatgttcgag cggtccgctc cgtgcggggg cagggcaaag cacgggcatg gaggcctcca
     3181 gcagggtggg cgaggggtgc agggcgatag ctaagcgcat ccgacggttg cccaggggcg
     3241 ttttcaagag cggaccagtg aaggtgtgtt cgaatacgtg caggggccca agtgccttac
     3301 gggtcgggcc agttgaaacc agcaaaatcg agtacccggc acccgggccg ctcgctcgta
     3361 cgacgatttt cagccatctg cggggcaggg cgccccgcag tagcacgcac gatgcggccg
     3421 gacgcgcctc gggttgctgg ggccgcacaa tgaccggttt cgccgcccct ggcatggccg
     3481 gcaccgccga ccgtgcgagg ctgccaccac cgatggcgca ggccaccccc aagccgcttg
     3541 gcgcgtcggc cgtgaggggg cgggcgagga tgccactcgc cacgaggttc tcgtcatcgg
     3601 gtgagcgagc acgcccagca gggacggatt cgcggacgct acggccttct gcggcatccg
     3661 ttcgtcggag tcggagcttt gtctcccggg tcgacaggat ccgtctgcct acgcgttctc
     3721 cggatcgcca acttgctcgg ctggcaagag ggtccggtgg gcgcacgtcc acccagtacc
     3781 gagagcggga ggcaccctca cgtcgcctcg gggttggagc accgggctca cggcaatccg
     3841 ttcgcgtggc gtttgtccgg ccgcctacgg tctgcgcatg acgaggacta cgccgaccag
     3901 accggtgtcc gtggaagcgg tgttcccgga gctggccccc taccgagccc ggactacccg
     3961 gttgcacccc agatcgggac ggcccggcat gcacgacagt cacgtcggtg ggccgatgct
     4021 gtggccggac ggcgaacaat ggccgctctg ccacgagccg caccagcacg agaccgaggg
     4081 gtacgcaccc gaggagatcc gcagcgcccg cgccgcaggc gtttggccgc cgagccggcc
     4141 gtggctcccc gccggaattc acggcctgga cgatccggtg cccttcgtgg ggctggccca
     4201 gttcttccgt cgcgacatcc cgaaccttct ctgcgggccc gacggcgcgg acctggtcca
     4261 gctcttccgc tgccccttca cccacggccc ggacctggag cgccgctacc gcctggagtg
     4321 gcgacgggca cgggataccg agcgcgcaca acgcttcctc gccgctccgc ccgaagtacc
     4381 cctgcttcga tgggagcacg aactcccgga gccgtgtgtc ctgcatccgg aagaggtgga
     4441 cacctatccc tgggcggagg acgacacgct gcccggcggg ctgatcgcgc gcatcgacgc
     4501 gtgggacgac gcccaggcgg cggaacacgg ccccgacgcc cccagctacc agtacgacct
     4561 gtcgatccct ccaggctggc gcgtcggtgg ccaccccagc tgggcgtcga cgggcccgat
     4621 ggccatcgac tgcgcgtcct gcgcagcccc gatgcggctg ctgctgacgg cagagggcaa
     4681 cgagatggac gccggctcgc acagctgggc gcctttggag gaccgtgacc cgtccatgag
     4741 ggggaaggca tcgatccgcg gtggccaacc cgtggcccaa cccactcggc tccgcttcgg
     4801 ccgggaccgg gacctgcaca ttttcacctg cccggccaac cctgggcacc cgccccaatc
     4861 ggtgctcgcc tagggggtct ggcagtgaag gaccacggcg gagtcgggta ccggaactct
     4921 ccgaacgcct cgaccgggtt cacgtcccgc gatcagggcc gggccaggga actgggcggt
     4981 cttccggact catcggcaag tgccggttcc gagtgctggt tcagctgttc ttgggcgctc
     5041 gcgtgacggc gggtgaagaa gacgcgtaaa cccccgccga aggcatcgtt caaggacccc
     5101 aagatccggg gctgggggcc agggcccaga ggcggcgccg gtcatcggaa tcggcctcgg
     5161 cggcaagtcg gcgcccctgc aaggcattcg cctgccagat gctccaccac ggctgcaccc
     5221 attcgcggcg ccgcgtcctg gacaggctgc tgctcggcga ggtagcccgg accgtgaggc
     5281 tgcggtcgca ggccctgagt cgcagctcgc gccgctgtcc gcagcccgct tccgaattgg
     5341 ccatctcgcg ccctggaaag gcctgttgac cggacggcgc tcgccctgct ctgtttcgct
     5401 gggtcagccc gccgcgaggc gagatgcgcc gcagcgccgc aagaccccag gtgcagcacg
     5461 gcggagcggc atggcgcgtg ctccgtcacc gcggatccac ctcgcaggca caccttcctc
     5521 actgacatga acatgcgttt ctcgccaacg gggccacggc gtggcggact tgacggaccc
     5581 tcatgatcgc ccgatggtac gctcccgtgc acatgccacg tgagctttcg ccttggcatg
     5641 gctacagcca ggggggctct ccgggcggtg acgtctgccc acagccgagg gtccgcttgg
     5701 gcagatttcg agagctggat tccagccgct caatagagag tcacatgacg aatttttggc
     5761 aggatccgac agtctgggcg cttgtcgtcg gcgtcgctgt cgccgccacc gtaatcaccc
     5821 gccagcgaaa aacgatcaag gcgctaagtc gtcacaagca gggcctgcac ggtgagctga
     5881 ccgcatcgca gggcagggca accgacctcc agtcaaccgt caccgagctg cgcaacggct
     5941 atgacgaggt ggtccgccag gccaaggaag aggcggagga ggccacaaac accgtactga
     6001 agtcggccat gcggaccctt cagggcctcg ccgcggaaca gcagcgggcc atttccggac
     6061 tgcagaagaa gtacggcgac tcggccgtgc tgcgggacat gctggacatc gaccacatga
     6121 actcgcaatt caaccggcgt gcgcagtcca tcgccgtgct gtgcggtggc tggctcggcc
     6181 ggcagcgcga ggccgcatcg gtccatgacg tgatccgcgg agcgcagggc cgcatccggc
     6241 actaccagcg catcgagatc gcctcgcggg tcgatttcgc ggtgaccagc cgtgccgtcg
     6301 aaccggtcgc gctggcggtg gccgagctgc tcgacaacgc cagcagctac tccgagccgt
     6361 ccaccatggt cgaggtcgag gtgcggacgg tgccccgggg catctgcatc gtgatcgacg
     6421 acgccggcgt cggcatgagc gaagaagaac ggacgaacgc caccgctctg ctgtccagcg
     6481 gctccgcggt gagcgtttcg gagctcggca accctcccgc gttcggtttc gcggtgatcg
     6541 gcgccctgtg tgcgcggttc gggttcacgg tcaccatcga cagcacctct ccctacggcg
     6601 gcgtccgtgc cgtggtgatg gtgccgaagg agttgttgac cgagatgccg gagcccaagg
     6661 cgcccacccc cgcgcgagaa gtcaatgccg ttgccgcgag ccgcggttcg ggtgccgcgg
     6721 cgacggaggg cgggctgccc cgacgacgga acaagcgcgg catggcgctc gtgccggacg
     6781 gcgggggcgg gtccgacgaa gcgccgcctg cccggtcggc agaggcgcgg gtggcggcca
     6841 taggcgcatt tcagcgtggc actctcagcg gccgaggtgc gagggtaccg gccgccgaag
     6901 gtgataccgc tgccgataca cacgaaggac tcgatgcccc gtgagcgaca acctgtcctg
     6961 gatgcttgag gacgcgctgc agattccgca tgctctccac gcggtcctga tctctgccga
     7021 cggcctccag atgtcccgta ccgcggagat cgaccggggc gatgccgaca aggtggccgc
     7081 cgccgtctcc ggcctgcagt cgctcagccg ggccgtcggg ttcttctgcg gtgagcaggg
     7141 aacgaactgg cgccagaccc tcatcgagtt cgacgggggc tgggtgttcc tgaacgccgc
     7201 gggcggcggc agctatcttg ccgtggcggc gtcggtggag gtcgacatgc aggacatcac
     7261 gtaccggatg cagcagctgg tcagccggtt gggcaaggcg atgtcgaccg gcctgcgcgg
     7321 cgacacggtc acctcatgac cgaacccggc cgtcccgagc ccgagttcgt gtccgaggcc
     7381 ggagaattag tccgtcccta cgtcatcacc cgtggccgtg acctgcccga cgaggacgag
     7441 ttcgccctca tcactctcgt cacgacggcc accgacgagc agcagcgccc ccagcggctc
     7501 tcgcccgagg agcagcagat cctggagatg tgctccagcg gctacctctc ggtcgccgag
     7561 atcgccgcgt actcccagct gccgctcggg gtggtcaagg tgctgctcag ttccctcgcc
     7621 gaagggggct atctcgtggc ccgggcgccg gtgccctcgg cccgtcccac caacaaggcc
     7681 ttgctcaagg aggtgctcga tggcctcaag gctctcactg tctgaggatg cctatgtgcc
     7741 cagcggcgca cagcagaccg cagtgaaaat tctggtcgtc gggcatttcg ccgtcggcaa
     7801 gaccaccctg atcggctcgc tctccgagat cacgccgttg tccaccgagg agcggatgac
     7861 gacgctgtcg gagcgcgtcg acgatctcaa gggagttcag ggaaagacga ccaccaccgt
     7921 cgccctggac ttcggccggc tgacgctgag cgagcgcatc gtcctgtatc tgttcggttc
     7981 tcccggacag cagcgcttcg tgagtctgtg ggacgacacg gcccgcggcg cgctgggcgc
     8041 cctggtcctg gtggaccccg agcgcctggc agactccttc gacatcatgg atcttgtcga
     8101 gagctacggc ctgaacttcg ccgtggccat caatcacttc gacggcagta cggtccacac
     8161 cccggaggaa gtccgggagg ccctcgacct gctgcccgac acccccgtcg tcaccatcga
     8221 cgcacgcgac gagaagtcgt ccgtcgacgc cctgatcacc ttggtccgct atctgtacga
     8281 gcgcgccgac ctggagcacg catgacaacc ccctctgtac ctccgcccgg ctgccccgca
     8341 cacggcagcg ggcagcgcat acccctggac agcgcggagt tcgccgccaa tccgcacgcg
     8401 tactaccgct atatgcgcga gctcggcccc accgctccgg tcaccatcgc ccccggcgtg
     8461 gaggcgacgc tggtgaccga ttacaacgcg gctctggaac tgctgcagga ttcgacgacg
     8521 ttccgcaagg actcgcgccg ctggcgtgcg ctggccgagg gctgcatccg ccccgacagc
     8581 ccggtcctgc cgctgctggc ctaccggccg aacgcgatgt tcaccgacgg cgccgagcac
     8641 gtgcggctgc ggcaggccat caccgactcc ttcgcccggg tcagcaacaa gcggctgagc
     8701 cggatcgtca gccaggcagc ccagttcctg atcaaccagt tcagcgcgcg cggcagcgcc
     8761 gacctgcttc gcgactatgc ccagcagctc ccgctgtacg tgttcaacga gctgttcggc
     8821 tgcccggcgg agatcggtga ccgggtgctg ttcggcatct ccggcatgtt cgacggagtc
     8881 aacgccgagg cggccagcca ggtcctgctg ggagcggtcg gcgagctggt ggccctcaag
     8941 cgcgcccggc ccggggagga cgtcacgtcc tacctgatgc agcatccggc gaagctgaac
     9001 gacgaggagc tggcgcacac gctggtgttg ctcctcggtg cgggcggcga gccggagggc
     9061 aacctgatcg gcaacgcctt ctacacgatg ctgaccaacg aggagttcgc gcgcctgggc
     9121 cagttcgaca aggctgtgga cgacactctg tggcgcaatg cgccgatgtc caactatgcg
     9181 ccccattacc cggtggtcga cacggacgtg gccggcgaca aggtgcgcgc cggtgacctg
     9241 gtgctggtgt ccttcggcgc cgccaacacg gcgctcgcgg accgcggtgc cgacacccgc
     9301 ggtcacctcg cctggagcgc gggcccgcac gcctgcccgt cgaaggagcc ggccaggctg
     9361 atcgccctga cgggcctgga gacgctgttc aacgccctgc ccgacgttga actcgccgtc
     9421 cccgcggaca gtctcgcctg gcggcccggc cccttcaacc gggccctcgc ctcgctgccc
     9481 gtccggttca gcgcagtcac cccgcgcgcc gcggccgcac cggagccgac ggccgtgccc
     9541 cgctaccagg cacctcccgt cgcggccggg cccgcgcccg cggcgccgca gcccaacggc
     9601 aaagcggggc gctggagcag cttcatgaag tggctgacgg gccagtagcc cacaccccct
     9661 gtgatgcgtg tggcacgggc agttgatata gcctgcatta aagatcaact tcctgggtag
     9721 aacggcgcgg agtgtctgga gtgacacaag atgaaggaga ggcagtggac ctcgcaagca
     9781 cttccgcgca cctatcggcg agcgcccgac cgggccccca gcgcaccatc cccagcagac
     9841 ggaacccgtg cgggggcggc gaccccgacc agccgcagga acgaataccc accgctcccg
     9901 gcagcggctt gagtttcgtc tcctccctgg tcggcggagc acgtccccgt actcccgcac
     9961 cgtccggacg cgttgcccgt cacgacgagc gaggcgcacg atgacctccc gcccgggccc
    10021 cggcataccg gcaaccggcc ccggtgagac gacgctcggc gaccacgtac tcggtcaact
    10081 gcggcgcctg ggcgccacgg tgggcctgag cgagagggac accgagctgt acggacaggt
    10141 cctgatcgag tccctcgcag gcgccgccca ccggccgctg tccctgccgc cggcctcgtc
    10201 cggcttcctc tccgacgacc acacccccgt cgagttctcc ctcgccgcca ctccggacac
    10261 cgccccggcc ctgcgcgtgc tcgtggagcc cggctgcgcc cacagcgaca tggccgacag
    10321 cggccgcgcc gggcttgcgg tcatccgtgc gatgggcgcc cgctggggct tcgacaccgg
    10381 ccaactcgac tccctcgccg acctgttcct ccccgctgcg gcccagggcc ccctggccct
    10441 gtggtacgcg ctcgatctgc gcgccggagg ggtcccagga gtcaaggtct acctgaaccc
    10501 ctccgcctcc ggcccggaaa acgcggctcg taccgtgcgc gaggcactgc ggcggctcgg
    10561 ctacggccgg gccttcgagc aactgcccca ggcggccggc tatccgttcc tcgctctgga
    10621 cctgggcgcc tggacgtcgc cccgcgtcaa ggtctacgtg agtcacctgg gcatgtcggc
    10681 cgatgacgcc tgcgggctca gccgtacctc aggagccgca gcggccgaca tccgctactt
    10741 cttccacgcg gcctcgggct ccctcgcgcc gtccgagagc ggcgcagacg acaccctgcg
    10801 gctcgtgcgg cggccggcgc tcacctgcca ctccttcacc gaaggcgaca gcgaaccgag
    10861 cggcttcacg ctgcacattc ccgttcgcga ctacgtgcgc gacgacgaag aggccctcgc
    10921 ccgcgccaca gccctgctga cccgggccgg catggacccc accgtcctga accggagtct
    10981 ggtggcgctc acgcagcgga gcctcagcga cggcgtcggg ctgatcgcct acctcgccct
    11041 ggtgtacgag cgcaaccggc cgccccggct caccgcctat ctgagctccg aggcctaccg
    11101 cgtccggccg ccgcaggagc agtacccgcg gggcacctgg atgcctgcgc aacgcacccg
    11161 gagcgtcctg gaccgcgggc accaggccgt cgcccacgtc agcccgtaca ccgcacagaa
    11221 ggaagtcacg tggagcccta ccgcatcaag gtcgtagaac ctcttgcctt caccacccgg
    11281 gaggagcgcg aggccgcgct gaagcgggtc gcctacaacc ccttcgatct gcgggccgac
    11341 gaagtcacca tcgacctgtt gagcgactcc gggaccggcg ccatctccgc agaccagctc
    11401 gcggcgggaa tgcagggcga cgagtcctat gccggcagcc gctccttcta ccggtggcac
    11461 gaggtcgtct ccgaactcac cggctacccc cacatcctgc cggcccacca gggccgcgcg
    11521 gccgaacgcg tcctcttctc ctcgctgctg cggcccggca cgagcgtgct gtccaacacg
    11581 cacttcgaca ccacccgcgc caacgtcgaa ctgaccggct gcacggcgta cgacctgccc
    11641 tgcgccgagg ccaaggacct cgacagcgac tttccgttca agggcaacat cgacctggtc
    11701 gccctcgaac aggccctgga gaacgcggac cggacgcccg ttggggccgt gctgatgacc
    11761 atcaccaaca acggtggcgg gggccagccg gtcagcatgg acaatctgca ccgcaccgcc
    11821 gaactgtgcc gccgtcacaa cgtgccgatg atcctcgacg cggcccggtt cgcggagaac
    11881 gcctggctgg tgacccagcg cgaacccggc tatgccgacc gcactccgcg ccaggtcgcc
    11941 gaggaagcct tccgcctcgc cgacggctgc gtcatgagcg cgaagaagga cgggatcgtc
    12001 cacatcggcg gcttcatcgg cctgaaggac cccgaactcg ccgagcggtg cgggggtctg
    12061 ctcatcgcca ccgaaggatt caccacctac ggcggtctgt ccggacgtga cctcgacatg
    12121 atggcccgcg gcctcctcga agtgaccgag ccggcctacc tcgccgagcg tgccgacata
    12181 gccgcctacc tcgccgcacg catccgcgag gcgggcgtcg acatcctgga gcccgccggc
    12241 ctgcacgccc tctacgtcaa cgccgggcgc cttctgcccc acatcccccc gcaccagtac
    12301 cccggcaccg ccctggtctg cgagctctac ctgcgcggag gcatccgctc ggcggagctg
    12361 ggctcgctgt acctcggcga agaggacgag cacggcaatc ccgtcaagac ggcgccctac
    12421 gagcttgtac ggctggctct gcccaggcgg gtctacaccc gcagccacta cgaccacgtt
    12481 gccgccacgc tggcagacat cgcgaagaat cctgaatcgg tccacggcta ccgggttacg
    12541 ggccagtccc cgatcctccg acacttcagc atcaaactgg agcccgtgaa gaccggagac
    12601 tgatcagcac gattcgttcg ttacgacgcg cgcccagcca tcagattcct gcaagcagcg
    12661 aaaatctttc ggctgggccg acaccggcca ccgcaccaag gcaattgacc ggtcgaagcc
    12721 gtgggaattt cacaacacgg tgttcggtgt tcggtgttcg gtgggactct ccaccccagt
    12781 gacgtgtccg gaggcggttt tcacttggcc cacctctgtc cacgtcggcc cggggaggcc
    12841 atctcgccca tgacgtgctt ggctcagggg ccaccgacct ccgccttgag ccgatctttg
    12901 ctgccttcat tggtgggggg ggggccagtg cccggcggct gcgctgagca ccactgggcc
    12961 ggcaccgcgg tcggaacccc gccacgggtc gcaggcccct gctccgggtg cggggaggac
    13021 cggttgttac cgtgcacgcg tgcctcactc ctcacgcgac acaccggaag gcgccggttg
    13081 ggggaactcc caactcggca cgtacaaaag cctgatgccc gctcgggtgg agaagctgtc
    13141 gtggctgaat cccgggacgc tgtgggccgc ccgaaacggt gtcctcgcct cctggttcgg
    13201 ggatccgacg ggccggactc gcagtcgctg ggtggagcag gccgtggccg cgggcgctcc
    13261 ccccgacgcg gtcgtgcgcc gcacggacgc cgaccacttc tcgttcatgg tcatcggtga
    13321 cacgggtgaa ggcgacgatc cccaatacgc cgtgataccg ggcttcctga aggtcagtca
    13381 gggcaccgac ttcgccgtgc tcgccagtga cgtgatctac ccagtgggga gcaccgacga
    13441 ctacggcaag aagttcttcc gcccttaccg gaactatccg gcgcccatct atgcgatacc
    13501 gggcaaccac gactggtatg agggcctgca gggcttcatg cgggtcttct gcgaggctcc
    13561 gccgctggcg tccgaggcgc gaccccgccc tctgacgcgg gcctggttgc gctcgctgct
    13621 ctggcacgca ccgggcagca ccgacgagca tcgtctcgcc gaggcgcggg ctttgcgctc
    13681 ggagccgtcc caacaagccg ttcagcccgg cccgtactgg gccgtcgacg ccgggccgat
    13741 acggatcatc ggcatcgaca cgggcctgct gggcaccctc gacgccgaac aaggccgctg
    13801 gctacgcgaa gtgtcgcgcg gcgaccggcc caagatcctg gtgacggggt ctccgctgta
    13861 cgtggacggc gagcaccacc cgtgcgccat cgagggcgga ggcacggtgg acgccatcgt
    13921 gcgcgacccg gaacaccact acgttgcggc gatcggcggc gacatccaca actaccagcg
    13981 gtatccggtt cgggtggacg gcgggcgcac gatccagtac gtcgtcgccg gtggcggcgg
    14041 ggcgttcatg cacgccacgc acacggtccc gcgggtctcc gtcgccggcg tgacagagca
    14101 cgacttccgc tgctatccgt tgcgcggcga ctcgttggcc ttctacagca ggctgtacgg
    14161 gcaccggctg cggatgccgc gcttcttcac gctgaccgag gccgaggccc tggcggtggt
    14221 cgccgaaagg ctgggcgtcc cgccgatccg cgcacagggg gagccggttc gcgtcacccg
    14281 gcgcatccgc ctggtcgcca gtctcctcgg cgcgggtcgg cggcccgacc gcaaggcgcg
    14341 tttgcggctg ccggtgcgca agctctacac gcagctcttc tcaccgggtt cggcgacgta
    14401 cagcccgccg ttcttcaaat gcttccttcg gctggacgtc acaccggccg cgatccgtct
    14461 gcgctgccac tcggcgaccg ggaccctcga ccacgagatc gatccgccgg tcgaggacga
    14521 ggtgaccatc ccactctctt gacgccgcgc ggcaggtgac cggacggccg ggcatctggt
    14581 cgcgatgccc ggcgtgacca tcctggacct cgacctgtcg gttggcgccg gccgtcgcgc
    14641 gcgaggacgc gaggagacgt cggcggggac ccatgccagg tacgcagctg agcccgcccc
    14701 cgcccggtcc gacggcgcgg tcatcgctac caccgtcctt cagcagcggg aggggcgctc
    14761 ggccccgggc ctggacttca tgccccgact gcaccggcca gggagacgtg gcgctcgggc
    14821 tcagcgcgat ccgggcggcc gggcagcgca tgccgagcgg tggcggcgac tgtaggtggg
    14881 acatgtggga tctcccggtg cgtggcgggc gtcacgcacc ctgatcagta cctcttcccc
    14941 cgtctcacgt tgcggtcgga ccgcgttgtc tcaagagtgc ggctttccct cggctgttgg
    15001 gggagcgcga ttggggtccg gtggtactgg agggtcagcc gatgcgcacg ccgaactgga
    15061 acggcatgtt gtccatggac tcgtagcgca cgaccgtgcc cgtccggggc gcgtgcagca
    15121 ccatgttgtt gccggcgtac aggccgatgt ggtgcaggtc gccgaagaag agcaccaggt
    15181 cgccgggctt gagctcgctg cggccgatcc gggtgcccgc gttggcctgc tcctgcgagg
    15241 tgcgcggcag gttgacgccg gcctggcggt aggcgtagcc ggtcagaccc gagcagtcga
    15301 aggccgccat accggtcttg gaccaaccgt acggcttgcc gagctgggtc tgggcggcgg
    15361 cgagagcggc ggcgccgacc tgcgaggcgg ggacgccctt gccgaggttc gggcgctcgg
    15421 tggaacggtt cgcgcgcgac tcctcggtcg ccagggccgc cttctcctgc gcggtcaggg
    15481 agttgagcag cttctgcgcg tcgccgagct tgccctggac ttccctcttc ttgtcgtcga
    15541 gcaccttgct ggtgtcggcg aggtccttca gcttgtcctg ggcctccttg cgctgctgcg
    15601 cgagggtccg ctgcttgccc tggatcttcg tcagggcctc ggcctgcgtg gcgctcaact
    15661 ggtcgagcga ggaggccttg tcgaggaagt cgtccgggtt ggaggagagg aacagcgcga
    15721 gcgaggggtc gatgccaccg gagcggtact gcgccgaggc cagcgaaccg aggccctggc
    15781 gcagggtgtt gagctcctgc tggccacggg cgacctggtc ctggatctga ccgatctgct
    15841 tctcgagccg gccctgcttc tccttggcgc cgttcgccgc ctcggtcgcc tgctcggcct
    15901 cgtcgtagag cttgtcgacc ttgctcttga cctcgtcctt ggtgggcttc gggtccgcgt
    15961 gggcggcctg cgaggtgagg gcgacgaccg cggcggcggt ggcgctgaac acggtcacac
    16021 gcgtacggct cgcgggcttg ggacgacggt gggaccccac gaaggcgaac tccttcttcc
    16081 tcaaccgccg agcagcacac tcgacgcgac catcacggac accccggatc ggaggtcgtg
    16141 ttgcggctga acttcccact cctcgctaac gattcgctag tgatcagcaa acgtgccgat
    16201 gtcagcgcga gccgccaggg agactggggg agccgagcac ccccaggggg gcaccgaccg
    16261 gcttcacggt cccgctaggc cgcgagtacg gactcttcct ctgcactgct gcggttatgg
    16321 cagctccctg ccggggaccg gggtaccacc gcccggggga gaggtgagcc agttggtgtg
    16381 ccgggacgag tgcggggacg tttcgatgag ttggatccgg ccggtggcca cgagaggcca
    16441 ccgctgggca gcggacgccc ccggcgctct cggggtcgag tgccaggagc gtgttcagcg
    16501 cgtcgcccgc gcaggcaccc agtcttgagc acggcagcac caggaggggc gggtgactgc
    16561 cctgatgctg gcaccagcgc taccgtctcg tgcgtctact gcgatgtgct gtcccgtggt
    16621 gtgggcggtg ccgtcccagt gatgatcatc aatggggtcg ctgtttggtg cagtgcggga
    16681 cgccagcgtc ctgaggaaga gcagcagctg ttgcggcggc cgcggacgtt gccgacgtgg
    16741 tccagcgcgc cctcgcagca cccgctgccg gaaacatctg ggggcggtgg ccctgtacct
    16801 gctgttcgag actggatcgg cctccacaac cagacgtgga agacgccgtc cctgggtgcc
    16861 aactgggctc accggggctc gacgttcggc cgagaggcgt accacatcca cccggacccc
    16921 acagtcgggc tacgcggcat tctccaacgc acggccaagt cgaccgcggt caacggcgag
    16981 gagttgccca ccgtgccggc cgagcctgcc cggcaacatc cggtacccca cggaccagca
    17041 cagccacgga ctttgtcggg ctcggttcaa cgccgacccg gcgggttggt cgcgtctttg
    17101 tagcagggcc ccgcaaaggt gcggagcgcc tcgtccaggc gctccaccgg gatatggggt
    17161 tcgtggtcgt ccctgtacac aataaagctc ttgtcgggca actgtttccg gatctggtgt
    17221 ttgtccagcc agtcccgggt cggttcactg ccttcgcgga accagacccg aactcccaat
    17281 atcccgaggc cgatgcgatc gaccgcactc cacttgatgg tgaaggtctg tttcacatcg
    17341 ttcctggtga ttgtgaggcc gtcgtcgttc acggtgatga tgtcggactt ggccgaatcc
    17401 gcctggaata ttcctttgag aacccagaag atcaacatga tgacgaagcc gcacaccccc
    17461 gccgtcacgt ggtttctcgt cgcgttggga aactcgaata tggactgtcc gaggttgctg
    17521 atccctgcgg caaggcccgc aaatgctccc acgaccgcgc cgttctgggc gtgccccttc
    17581 accacttggg aaaagatctc cgacgaaccg agggagacgg tggtctgctc cttcccgctg
    17641 cccttctctc tctcgggctt ggtcggcggg gtgtccgggt ggtggtctag cagggcgatg
    17701 ccctcggttg agaagggccc aagccccggt ggctcgtcgt gagggctcgc ctcgtatctt
    17761 ccgcacaaga ctgatgatcc gttgcagtgc cgttgtgtcc tctgccatga ctactacgac
    17821 cgggacgaag cggatcacag ggatctgacg gtcatcgagc atgtgcagta gcacggatgg
    17881 cacgtcgtga tggtgcccga agacgaagtc ggtcctggat tcgcctacac gatcgccctc
    17941 gcgcacaacc acggcggacc tgaactcgcc atgttcgggc tcgacgttcg cgccatgcaa
    18001 cgcatgctca acacacccgg aaagagatcc gccgcaggcg aagtactgga agacggccag
    18061 agtcatcctg atgttgtcga cgggcaccga gtcgcgctca ggcgggttga tcgcggctgg
    18121 taccgcacct tcttcgggca ggctgtcggc ttctaccggc ggccgcctct ccctgtagtg
    18181 caaatcgcgt ggccggatgc cgaaggctgt ttccactggg atgagcaggc tgaagacagg
    18241 catcgggagt tacagcctca gttgtggctg cctccgagtg accatcctgt cggaatctgg
    18301 acgactgaac tctgaactgc gacctggtgg gtggagggcc agcatccaga acgatgtcat
    18361 gcggtgcgac ggctcccctg aaaccgtgat gacgtcaact cgcccgcgtc ataatccttc
    18421 agcaggtgag tgtgatcgag gtggagccgg gtcaggtcga gctcgtcgtt caaggggcgg
    18481 gtgcgtcggc gtgctcggtc cggttgtccg gctggtcacg tgaggatgag tatgcggcct
    18541 cgttcgccgt ggaggctatt aatgaaggtg tgcgtgcgcg gcttgcgacc gctactgtca
    18601 ccgtctgggc caacatggac gggttcttcg acagcctggc tcgcgacttc cgtggctggg
    18661 aaggcgagcg tgtctggacg agcagtcatc tggaggtgac agtggccttc ggctcgggcg
    18721 gccatgtgta tctggactgg accctgcggt ccggcttctt cccggggaac tggaggtgca
    18781 ctgtgacgac cgttatcgaa gctgacgagt gaatgacgtc tgtggcctct gagctgcggg
    18841 aattcctgca gctggagtag atttcagtag ggcttcagca agctcacccg gctgcctgct
    18901 gttctctgcg acgctcgagt ggtggcaagg ccgtaggagt cggtgtcggt ctcgatgagg
    18961 ttgtcaccga aggcgaggcg gtcgacgatg gccgagcaga gcctctgctc ggcgaaggtt
    19021 agggtctttg cgcactccgg ccccgcccgg tctggcagat ccggggtgcc aggaaaggct
    19081 ctacgctccc cagaggcagg gttccgcagc gcacttcgcg tacgtcacat gtgccgccct
    19141 ggctgattac gcaaagggca gctggacgac tgaaaggcgc tgagtgcccg tccgtggctg
    19201 ccttaccctg gagcccgcgt tcagcgctct gactatgtgg catggccgcg tcgccgagag
    19261 tgcgtgcggt gattgtggag caggccgggc tgatgctcag agcggatcac gacatttgct
    19321 tgtgcttatt tgaatttttc gcattcgcac gagcttttgc agctggatgt cactgctccg
    19381 cttccgccgg aagagttgct gtcagcggcg tcttaggagc ctatgccggc cccgacgttc
    19441 agcactgtct gtgcgtccct acggactttc gacgccgtgc cccaggaggc cctcttccga
    19501 gttgccggcg aagttgcaga gctggtcggc gactcgtcca ccgacgcgac tccctggcct
    19561 tctctgggcg aggtccctgg agcaagcgca gtccgttacc gcgctggtcg tcaacagcac
    19621 ccggcaagac tgcagttcag agaaagatgt tatggcgcgg ggagcctgtc ggctgatgtg
    19681 tacagcgtgt ccaggagttg tctgtggtga gtatgggagt gctgctggaa gacgccggtg
    19741 gtcaggtcgg ggcggccggg tggggggtcg ggcagggtgg ctgctgcgct gggaggctgt
    19801 gccagcgcgc cagccagtcg gctgagagca gccaatctat cttggtcggt gccgccgtag
    19861 ccctggccgg cgaggtcttc ggcggtgagg gtgacaaccg ccgggtggtg gtgcagccag
    19921 gcagtctgac gggctgaagt gtggccgggg aactggggat cggtcagggc gtgggtgagg
    19981 cgtgcatcca tggtgggtag cgccatcagg atgtcccggt agaccaggta ggccggaatg
    20041 gtgcctacgc gtccgtcttc gcggcacagg tgctgaatca ggttggtgat ccggtcgccg
    20101 ggatcgcgat ggaggtagaa gatcgccggt tgtccggtcg cggtccagtg gcgcagcagt
    20161 ccgccgtcga cgcgagtgag gtccagttcg gtggtgtacc aggcgtttcg gccggggagc
    20221 atgctgatca gttgggcgtc gtcctggcgg gccaggtgtt cttcgacagc tgtgtcgagc
    20281 ggtgtgtggg cggtgacggg ctgaccgagg cgtttccacc acacgcgcca cagggccagg
    20341 actgcctggt ggtaggcgtt ctggagctgg ttagtggtgt tggggtcgtg atggtggtcc
    20401 tgcagaagct gtacggcttc tcgttggccg taggcggctt cgaggagggg aaagacttcg
    20461 ccggggcggg cgggagctgg cgactcggcg ggtgcgttct gagtgccgcg gggagtgtat
    20521 cccagcgcgg tgatggccgg gtgcagcagt tcgataccgc tgccgggcgg ggagacgacg
    20581 accagttgct gcatgagggt ggtggggttc ctgacttggg cagctcgggc ccgggcggct
    20641 cgacgtcgat acgtcaggcc gggaccgggg ccagggccgg tcggggtccg ggaagagcgc
    20701 cggcgggctg tgtggcgagt agcaggtctt cgtaccggtg gtgggtgcgc agcgtggtgc
    20761 ggtgttccag cagggcagcg gctcgccggc cttggccgga ttccgccagg gcgcgggcaa
    20821 gggtgtcttg gacgatctcc cgttccaccc tcacgcctcc cagccggtcc acagcatggt
    20881 ccagtggctc caacagggtt accgcctggg tgtgttctcc ggtgcacacg gccgccagag
    20941 ctcgtaccac gggagccaat acctcccgga acacctctcg ttcgtcgcgt tcggcggttg
    21001 tcgcgaggcg ctgcatgtcc tccactgcgc cttcacaggc caaggcaagg gccaggttga
    21061 aggtgtgcag agtgtcggcc atacctttcg gtgaggccag cagttcgcgc acgtggtcca
    21121 gttctgacag gcaggccgga ctctgtccgg ccagcagaag ccgccagttc gtggccgcgc
    21181 gcatgcccac atcggactgc tgcagagctt cgtcggcacg ccgacgcgcc tgggagaagt
    21241 ccccgctggc gatgcac
//
