LOCUS       NZ_CP070373            11926 bp    DNA     linear   CON 18-MAY-2024
DEFINITION  Streptomyces sp. MBT27 chromosome, complete genome.
ACCESSION   NZ_CP070373
VERSION     NZ_CP070373.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MBT27
  ORGANISM  Streptomyces sp. MBT27
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S.S., Hankemeier,T. and van
            Wezel,G.P.
  TITLE     Discovery of novel glycerolated quinazolinones from Streptomyces sp.
            MBT27
  JOURNAL   J Ind Microbiol Biotechnol 46 (3-4), 483-492 (2019)
   PUBMED   30729343
REFERENCE   2  (bases 1 to 8400223)
  AUTHORS   Machushynets,N., Elsayed,S., Carrion,V.J., Siegler,M.A., de la
            Cruz,M., Genilloud,O., Hankemeier,T. and van Wezel,G.P.
  TITLE     New from old: conjugation of actinomycin X2 and anthranilamide to
            form the novel actinomycin L in Streptomyces sp. MBT27
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8400223)
  AUTHORS   Du,C., Machushynets,N. and van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2021) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   4  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S., Hankemeier,T. and Van
            Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2020) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 1.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011751035.2-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 03:23:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,575
            CDSs (total)                      :: 7,482
            Genes (coding)                    :: 7,345
            CDSs (with protein)               :: 7,345
            Genes (RNA)                       :: 93
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 39 of 137
            Pseudo Genes (incomplete)         :: 114 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:14:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3893070
            Orig. end                         :: 3904996
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MBT, Institute of Biology
            Leiden, Leiden University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11926
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="16"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11926
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11926
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6926](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6926)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(167..181)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.25"
                     /tool="antismash"
     misc_feature    complement(860..874)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.37"
                     /tool="antismash"
     gene            2422..2934
                     /locus_tag="GWQ54_RS17215"
     CDS             2422..2934
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GWQ54_RS17215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167151319.1"
                     /transl_table=11
                     /translation="MATNCRKSLEQWELMQSEARVVRDATVLLEFARTGQLGFLALGAS
                     AQEVEDGMGKPSGVADSAARMAAYSDVQLGQSPSGIVGLISVEPDPFTGLLVLPPPLGS
                     GMQTPAPGLEVFRAYLGRQGLGLESRELYTPGEFWWVVPGSEVSMAFDEQGELQTIASS
                     DRDLLAW"
     gene            complement(3270..4439)
                     /locus_tag="GWQ54_RS17220"
     CDS             complement(3270..4439)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /locus_tag="GWQ54_RS17220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_167151317.1"
                     /sec_met_domain="p450 (E-value: 3e-16, bitscore: 58.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGSIPFRKTLDERTAASQGSAQPVGTIVPMRMGTAGVGLLVHD
                     HREATRILSDVDTYGSAGELMASVFDEPTETDADAMSDMVFAVTQTDGEEHRRVRRALR
                     RVIDTRVRDVSDQLIQDVQHTIENALALGRIDAVASIGVPIADSVMSRLLGLDENLVSA
                     LRTAVYQGYPVRELDATLVDWVHDRRADPADDLVSDLMAELPSLEDRQVAANTRLLALS
                     GVEVLAALITNGILCLAQDPRTQSLVRDDETLLPGLIHEVQRYASPIARGIYRMTKEPS
                     VIGEYAVPAGTLLVIGVDVCNRDTSVLADAHRFDPTRGRDFDHLTFGHGRHSCLGIPVT
                     RLIAAQALRTFLSGTTSFGLTVEASELRFHDTAVMNGLVELPIWVERAS"
     gene            complement(4432..5007)
                     /locus_tag="GWQ54_RS17225"
     CDS             complement(4432..5007)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.3"
                     /locus_tag="GWQ54_RS17225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5825 family protein"
                     /protein_id="WP_167151315.1"
                     /transl_table=11
                     /translation="MVTKQTAERRSNRRRLFAPVNLQSVRSRENLVTLTRSGYAGVRLV
                     GHFAMVEMGDRELVSLIALLRDARGVGLRVSWSGDCGALEVGRLRHLDPPRQSDGTFAW
                     SAQQGEALVVRRGPTFLAVEDTRYGERRRVDIDRSEPAAAVLDEIRWGHTITPVEAASL
                     HALELHDFVFRSGDHCVGIAVRQGVWCV"
     gene            complement(5001..6926)
                     /locus_tag="GWQ54_RS17230"
     CDS             complement(5001..6926)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="GWQ54_RS17230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_167151313.1"
                     /sec_met_domain="TIGR03975 (E-value: 2.3e-150, bitscore:
                     500.6, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELLQIGHPRPVLDPVPPVVHEDALSVALVCMPWAVVDMPSLALS
                     TIAPLFEQRGEVEKLDVVYGNIRWLDFLVREVGLKRDHYELIVDAEVFGVGEWVFSSCF
                     RPGVDPRHTSFYELVRDSDNADAYCAMFRAAPAFIDELARDLVASGVDVLGLTTTFDQN
                     IASFALTHRVKELSPSIVTLLGGANCDGVQGTAVHRARAEFDYVIRGEAERSIAPLIAH
                     IAGCRGSDEHGHEERLRAVPGLCWRSGTDGASVSNPIGPTPVSMNAVPEPVYDHYFRDL
                     RKSPAARLIDVKIPLEASRGCWWGAKHHCTFCGLNGSAMAHRAKPAERVQAEASRAVRR
                     HNVLDLVFSDNILAPDHVVRLTTDMALPAEWDVRLFAEVKSNLNFGQLESLARAGFTQL
                     QPGVESLCTPILRIMRKGVTGWQNVRFLRDCATCRIYPSWNVLVGFPGESDLDYTALID
                     SLPSLHHLTPPAGVFPIKLERFSPYFDDPELGLQVLGPSRNLVSAYEGVVDALDDFVYV
                     FESEPAGISPETCGLLREAVANWKAGASNSRLVALPAPDDSLVVVDDRSTWPSREVVLQ
                     PGLEAEAYRALAKGKTLPALASTIGSLGMSVREGQLEELVGHWTELGIVFHEDATYLAL
                     ATGLRW"
     gene            complement(6952..8220)
                     /locus_tag="GWQ54_RS17235"
     CDS             complement(6952..8220)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057931.1"
                     /locus_tag="GWQ54_RS17235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_193587819.1"
                     /transl_table=11
                     /translation="MTTTEALDGSAAIDDAARSVARPFYVYAVLANSLFQRGVFVLFLQ
                     QRGFSAGQVAILQTLIYLVSGLAELPTGVIADRIGRRASIVIGQVLIAGCLLGQVTSSN
                     YWVFLTLFMGQGVGMACVSGSDTALLYDLLVRRGATAGYVKIKSRFTMLGTVTSGVAIV
                     LGGQLQRFSWGIVYLGSASCLVLAVVVLMSRVPEIRGADAVDEQDGAEKGPVDATAWRA
                     MLRVATPALVTLVVVSGLMHATLTPYIIFTQKTLSDQGAGTALVSVVISAGFFVGGVVP
                     LLSDRANRSIGYRVIVPVSLLTLAAALGLSGLGLVWVTITAFLVLVGIPEITAVLVDNV
                     LNEAVPSRHRASLLSMISFVESVLIGTGYLVVGTLMDGLGSSVGMATYAAVPLLACLLW
                     LPVLFRGARVTTEIEKPADPEAS"
     misc_feature    complement(8488..8499)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            complement(8496..9674)
                     /locus_tag="GWQ54_RS17240"
     CDS             complement(8496..9674)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004000652.1"
                     /locus_tag="GWQ54_RS17240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_167151310.1"
                     /sec_met_domain="p450 (E-value: 3.1e-17, bitscore: 61.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRAPGCPLDPAPQLAELQERAPLVRVRLWDGSTPWLVTRHAEQC
                     ALLSDRRVSADWMQPGYPFHSQFLQEHHEEGQFLTVMEGPEHLRLRRMIARPFTPRKVE
                     QLRPTVQQVVDDHIDALLAGPKPADFVSDFALPIPSITICHVLGVPYEDHDFFHHAIRT
                     MTSVDAPDDVVSKSERELYHYLAKQIGRKLAAPEDDLLSQLATERLSTGQLNRHQLVML
                     VLFLLISGHETTASMIALGTLALLQHPDQITVLKDSDDPQVAAAVDELLRYLTTVQPGR
                     RRVAVEDIEIAGQVIRAGEGLILPEEIGNRDESVFPAAGVLDLRRDASQQLAFGFGVHK
                     CTGQPLARLELQVVFAALFRRIPTLTLAVDLGDLPFMDDGLGYGVKKMPVSW"
     gene            10662..10823
                     /locus_tag="GWQ54_RS17245"
     CDS             10662..10823
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GWQ54_RS17245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167151307.1"
                     /transl_table=11
                     /translation="MAQELQERSLTVTDEAEPDMVLFSDEEGLDLADDVELITLSSATL
                     GAIVARRC"
     misc_feature    11643..11656
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
ORIGIN
        1 ttcgccgact cgctgcgagc ggccgccccc gaactcgtcg ccattcccaa cgcggtcccc
       61 agcgggttcc gcccccgctc cgacctcggc ggccggacca tcgtgctcgc cgcgcgcatg
      121 accccggaga aacagctcga ccacgccatc gaggccttcg ccacgctggc cgaggaccac
      181 cccgactgga ccatgcggat cttcggcgac ggcccgcagg aggtcaacct ccgccgtctc
      241 atcgacggcc tggccctcca cgaccgggtc cagctcctcg gacgctccgc ccagatggaa
      301 caggagtggg ccaaggcagc gctcgcggtg ctcccgtcac gcaacgaggc attcccgctg
      361 gtgctcctgg aggtcttcgc ggccggtgtg ccggtcgtcg cctacgacat cgtcaccggc
      421 cccgccgaga tcatccgcca cggcgtcgac ggactgctcg tgcccgccgc cgacaaggac
      481 tccctcgccg cggccatggc gaagctgatg gaggacgacg acacccgcca cgcgtacggt
      541 gccgccgccc gcgccggtgt ccacgaacgg ttcgggtcca ccgagatcgc cggccgctgg
      601 gaggccctgt tcacccgcct cctggcgcgg cgcgacgacc cctggcggct ggcccagcgg
      661 gccgaccgca ctgcccggcg gatcgctgcc ggcggcaccc gcagcttcaa catcagcgcc
      721 cctgtcacca cgctcgcgca gtccgcggac gagcagaagg cacgcgaggt gctgctccag
      781 gcccaggacc gcagcggcac cctggttcgt accgcggggc gtctcgcgga agtacgcgac
      841 gacgtactgg ccccccgcat ggccgagtgg aacctcgaac tggccaccga cgcgctcacc
      901 gcgcacggcg tgccctacac gctgctgcgc gacggcggca cctcgtaccg gatcgccgtc
      961 gaggtcgaac gccgggccca gacgctggac gccctggcga ccgaactgca cggcaagccc
     1021 gtctacgccg aactcatcgc acccaacggc gccgcccccg gcgcctggct cgccgagcgg
     1081 ctgggcaacg ccggcgacgt cgccggcgtc cgcctcttca agccggtcac cacgaccggc
     1141 cgcacgctgc gctacgggcc ggccttcggc tgcaccatcg agttctgggc ggtcagtgac
     1201 gaggacgagg agtttcccgg ctggcgcaag gcgccccggg ggtccaccct cgtgggcccg
     1261 cgcctgccca gcctggaggc gaccgcgagc ctgcgcgtgg gcgagcgcga gcaccccacc
     1321 gccgcagcct tcacgggccg gctcatgaac gacatcgact tccccgtcga cgtcgtctac
     1381 acctgggtcg acgacacgga ccccgcctgg cagcgacgtc gcgacgaagc ccggcgggcc
     1441 gccggtatcg ccacgtcggg cggtgccgac agtggggacg tccgcttccg caaccgtgac
     1501 gaactccgct actccctgcg ctccttggcc atgtatgcgc cctgggtacg caccatccat
     1561 ctcgtcaccg acgaccaggc gcccgactgg cttgagcttg cggacccccg tatcaagctg
     1621 gtcagccacc gggagatctt cgccgacccc ggctggctcc cgacgttcaa ctcccatgcc
     1681 atcgaaagcc agttgcaccg catcgacggt ctcgccgagc acttcctcta cctgaacgac
     1741 gacgtgttca tcggccgacc ggtgactccg caggccttct tcgacagcaa cgggacggcg
     1801 caccacttcc gttcgcccac cgccgtgccg ccgagcgagc tctccgacga cgacgagggt
     1861 tacttcgcgg cagcgaagaa caacaggacc gtcctgcagc gcgagttcgg ccgcatcgcc
     1921 acccacagct tcctgcatgc tccgcacccg ctgcggcgca gcgtcctcga agagatcagc
     1981 gagaagttcg gcgaagaagt cgcggcaacc gccgccaacc ccttccgcgc cgtgacggac
     2041 ctgtccgtca cctcctcgct gcaccaccac tacggctacc tcaccggccg gtccacgccg
     2101 tcgtcgatct cctgcaccta cgtcaacgta ggcaacgccc agcaccacac cgtcctgagc
     2161 cgtctcttgg ccacccgggg ccgcacggtg ttctgtatcg gggaatccgc ggaagcggag
     2221 gtgtccgtga cggaacagga ccgcacactg cgcgccttcc tggaggccta ctacccggtg
     2281 ccctcaccgt tcgagcgcgc ctagccacgc gatttgcagc agcgtccgta cccccgcggg
     2341 tggaaccttc ggggggacgg acgctgctgg gcgcagataa gcccgtcaac agcgaacccg
     2401 gacggttcca ccgggtatcc gatggcaacg aactgccgga aaagtctgga gcagtgggaa
     2461 ttgatgcagt cagaggcgag ggtggttcgt gacgccacag tgctcctgga gttcgctcgc
     2521 accggtcagc ttggctttct tgctctgggt gcctcagccc aagaggttga agacggcatg
     2581 gggaagccga gtggcgttgc ggacagcgca gcacgcatgg ccgcttactc agacgttcaa
     2641 ctagggcaat ctccgagcgg aatcgtgggg ctcatttccg ttgagccaga ccccttcacg
     2701 ggcctgctgg tgttgccgcc gcccttgggc tccgggatgc agacgccggc ccctggtctc
     2761 gaagtgttcc gcgcgtatct cggaaggcaa gggctcggcc tggaaagtag ggagctctat
     2821 acgccgggcg aattctggtg ggtggtgccg ggatctgagg tttccatggc gttcgatgaa
     2881 cagggagagt tgcagacaat tgctagctcg gaccgagacc tgttggcctg gtagccgagt
     2941 caacgctttc gtagcagatg ccatttcagg atggcggggc cctccgtagc agatcaaggg
     3001 tcttagcgta gctttttgcg cagcgagcaa gaaaaagcga tgggggaggg gtttctccga
     3061 tatgggggaa tccgctgtcc gggcagagca tcgccaactc tctgcgcaca cctgagtgtc
     3121 tccacccgcg tatggatgga aaagggaagt tattgacgag gcgcgtcgtc agagtccgcc
     3181 caagctcgac cggaacgttc cgcgggaacg tatgacccgt aacgggtcac tttgcagcgc
     3241 gaagcaacgc actcgggacc acgagaccac taactcgcgc gctcaaccca aataggcagt
     3301 tcaaccagcc cgttcatgac cgcagtgtcg tggaaacgca gctccgacgc ctccacggtc
     3361 aggccgaaac tcgtcgttcc cgagagaaac gtgcgcaggg cctgtgccgc gatcaggcgt
     3421 gtgacgggaa tgcccaggca tgagtgccgc ccatgaccga acgtcagatg gtcgaaatcc
     3481 cgaccgcggg tggggtcgaa cctgtgagcg tcggcaagta ctgatgtgtc cctgttgcaa
     3541 acgtccaccc cgatgacgag cagagtacct gcgggaactg cgtactcgcc tattacggat
     3601 ggctccttcg tcatccgata gatcccccgc gcgatcgggc tcgcgtaccg ctgcacctca
     3661 tggatgaggc cgggcagcaa cgtttcgtca tcgcgcacca ggctctgagt cctcgggtcc
     3721 tgcgccaggc agaggattcc gttcgtaatc agtgcagcca acacttcgac gcccgacagc
     3781 gcgagcagtc gcgtgtttgc cgcgacctgt cgatcctcca gagaaggaag ctctgccatc
     3841 aggtccgaca cgaggtcatc ggcggggtcg gcacgcctgt cgtgcaccca gtcgacgagc
     3901 gtggcgtcga gttcgcgcac cgggtatccc tggtagaccg cggtcctcag agccgagacg
     3961 agattctcgt cgagtccgag cagtcgcgac atgacactgt cggcgatggg cacgccgatc
     4021 gacgcgaccg catcgatcct gcccagggcg agagcgttct cgatcgtgtg ttggacatcc
     4081 tgaatcagct ggtccgacac atcgcgcacg cgcgtgtcga tgaccctccg aagggcacgc
     4141 cgcacacgac gatgctcttc cccgtcggtt tgggtgaccg cgaagaccat gtcggacatc
     4201 gcatccgcat ccgtctcggt cggctcgtcg aaaacgctgg ccatgagttc gcccgcactg
     4261 ccataggtgt cgacgtcgga caggatgcgg gtggcttcgc ggtgatcgtg gacgagcagc
     4321 cccacccccg ccgttcccat gcgcatggga acgatggtgc cgactggttg cgccgatcct
     4381 tgcgaagcgg cggtccgctc atcgagtgtc ttgcggaagg ggatgctgcc ttcagacaca
     4441 ccagactcct tgccttacgg caatacccac acagtgatcg ccggaacgaa agacgaagtc
     4501 gtgcaattca agagcgtgca gactggccgc ctccacgggg gtgatggtgt gcccccaacg
     4561 gatttcgtcg agaacggctg ccgccggttc cgagcggtcg atgtcgacgc ggcggcgctc
     4621 cccgtagcgc gtgtcctcga cggccaggaa cgtcggaccg cgccgcacga cgagggcttc
     4681 tccctgctgc gccgaccacg caaaggtgcc gtccgactgc cgtggcgggt cgaggtggcg
     4741 aaggcgtccg acctccaggg caccacagtc gccgctccac gaaacccgca agccgacgcc
     4801 gcgcgcgtcg cggagcagcg cgatcaagga gacgagttcg cgatcgccca tctccaccat
     4861 ggcgaagtga ccgaccaggc gaaccccggc gtatcccgac cgggtcaggg tgacgaggtt
     4921 ctccctgctc cgcaccgact ggagattcac cggagcgaag agccgtcgtc ggttcgaccg
     4981 ccgctctgcc gtctgtttcg tcaccatcga agtcccgtcg cgagcgccaa gtaggtggca
     5041 tcctcatgaa aaacgatgcc gagctccgtc caatgcccga cgagctcttc gagctgacct
     5101 tcccgcactg acatcccgag cgagccaatc gtggaagcga gcgcgggaag cgttttgccc
     5161 ttggccagtg cacggtaggc ctcggcctcg agacccggtt gaagcaccac ctcacgggag
     5221 ggccaggtgg accggtcgtc gacgacgacg agcgagtcgt ccggcgcggg cagggcgacc
     5281 agccgagaat tcgacgcgcc cgccttccag ttcgcgaccg cttcgcgcaa caacccgcac
     5341 gtctcgggcg atatgccggc gggctcggac tcgaagacgt agacgaagtc gtcaagggcg
     5401 tccaccaccc cctcgtaggc gctgaccagg ttgcgcgagg ggccgagcac ctggagaccg
     5461 agttcaggat cgtcgaagta ggggctgaag cgttccagtt tgatcgggaa caccccggcg
     5521 ggcggcgtca gatgatggag ggacgggagc gagtcgatca gcgccgtgta gtcgagatcg
     5581 ctctccccgg ggaaacccac gagaacgttc cacgacggat agatcctgca ggtggcacag
     5641 tcgcgcagga agcgcacgtt ctgccacccg gtcaccccct tgcgcatgat gcgcaggatg
     5701 ggcgtgcaga ggctctcgac ccccggttgc agttgcgtga aaccggcgcg cgcaagggac
     5761 tcgagctgac cgaagttgag gttcgacttc acctcggcga acaaccggac gtcccactcg
     5821 gcgggcagcg ccatgtccgt ggtcagccga acgacatggt cgggcgccag aatgttgtcg
     5881 gagaacacca ggtcgagcac gttgtgccga cggacggccc ggctggcctc cgcttgcacg
     5941 cgctcggccg gctttgcgcg atgagccatg gccgacccgt tcaagccgca gaacgtgcaa
     6001 tggtgcttcg cgccccacca gcatccgcgg ctggcctcca gcggaatctt gacatcgatg
     6061 agtcgagcgg ccggcgactt ccgcaaatcg cggaaatagt gatcgtagac gggctctggc
     6121 accgcgttca tggagacagg cgtcggcccg atggggttgc tcaccgaggc tccgtcggtg
     6181 ccggatcgcc agcacaggcc gggcacggcc cgcagccgtt cctcgtgccc gtgctcgtca
     6241 ctgccacgac aacctgcgat gtgggcgatg agtggcgcga tcgagcgctc cgcctcgccc
     6301 cggatcacgt agtcgaactc cgcgcgggcg cggtggacgg ccgtaccttg tacgccgtcg
     6361 cagttcgccc cgcccagcaa cgtgacgatg ctcggggaca gctccttgac gcggtgcgtg
     6421 agcgcgaacg aggcaatgtt ctgatcgaac gtcgtcgtca gccccaggac atcgacgcct
     6481 gacgccacga gatctcgggc cagctcgtcg atgaaggcag gggccgcgcg gaacatcgcg
     6541 caataggcgt cggcgttgtc actgtcgcgc acgagctcgt aaaaactcgt gtgccgtggg
     6601 tcgacgcccg ggcggaaaca actggagaag acccattcgc cgaccccgaa cacttcggca
     6661 tcgacgatca gttcgtagtg gtcacgcttg aggcccacct cccggacgag gaagtcgagc
     6721 cagcggatgt tgccgtagac gacgtcgagc ttctccacct cgccgcgttg ctcgaacagc
     6781 ggggcgatcg tactcaacgc cagtgagggc atgtccacca ccgcccaggg catgcagacc
     6841 aacgcaaccg aaagggcatc ctcatgcacg accggcggga ccggatccag caccggtcgc
     6901 gggtgcccga tctgtagcag ttccacttcg cctcctcatc gtctgcctgg ttcaggacgc
     6961 ctctggatcg gcgggcttct cgatctcggt ggtcacccgc gcacctcgga agagcaccgg
     7021 gagccacagg aggcatgcca gcagggggac cgcggcgtag gtcgccatgc cgacactgga
     7081 gcccaatccg tccatgaggg tgccgacgac gagatagccg gtaccgatga gcaccgactc
     7141 cacgaacgag atcatcgaca gcaggctcgc ccggtggcgc gacggcaccg cctcgttgag
     7201 cacgttgtcc acgagcaccg cggtgatctc gggaatgccg accaggacca ggaacgcggt
     7261 aatggtgacc cagacgaggc cgaggccgct gaggccgagc gccgcggcga gcgtcaggag
     7321 ggacaccggg acgatcaccc ggtacccgat gctccggttc gcgcggtccg acagcagggg
     7381 aacgactccg ccgacgaaga atcccgccga gatgaccaca ctcaccaaag cggtgcccgc
     7441 tccctgatcg gagagggtct tctgcgtgaa gatgatgtac ggggtcaacg tcgcgtgcat
     7501 caacccggac accacgacca gcgtcacgag cgcgggcgtg gcgacccgga gcatcgcccg
     7561 ccacgcagtg gcgtcgacgg gtcccttctc ggctccgtcc tgctcgtcca ccgcgtccgc
     7621 gccgcggatc tcgggcaccc gtgacatcag caccaccacg gccaggacga ggcaggacgc
     7681 cgaaccgaga tagacaattc cccaggaaaa cctttgcagt tggccgccca ggacgatggc
     7741 gactcccgag gtgaccgtcc cgagcatggt gaaccgggac ttgatcttga cgtacccggc
     7801 cgtcgcacca cgtcgtacga gcaggtcgta cagcagggcg gtgtccgaac cggacacaca
     7861 ggccatgccc acgccctggc ccatgaacag cgtaaggaac acccagtagt tggaggacgt
     7921 cacctgaccg agcaggcatc cagcgatcag cacctggccg atcacgatgc tggcacgcct
     7981 gccgatgcgg tcggcgatga ctcccgtcgg cagctccgca agcccactga ccaggtagat
     8041 cagtgtctga aggatggcca cctgcccggc ggagaagcct cgttgctgca gaaaaagtac
     8101 gaacacgccc cgctggaaga gcgagttggc gagcacggcg tatacgtaga aggggcgcgc
     8161 gacgcttcgt gccgcatcgt cgattgcggc cgacccgtca agggcctccg tggtggtcat
     8221 gttgcgggat ccccctggtg gctccggtag tcatgggtgt tgggcctccg ccgtcgaccg
     8281 gaacctacaa cccgtgctgc gcgcaggact tggggccgcc gatccgggaa ccgtcgccgg
     8341 cgccgcggcc attctcggtg gaaatgccac gtgtgggaac cggcggcgtg cgtctcctgg
     8401 aggcccgcca cggccggcgg actctgctcc ggcgggccgt gcccgatccg aaacgactcc
     8461 actccgactg ctgtcaccgg ctcggcctcg ttcacttacc agctcacggg catcttcttg
     8521 acgccgtacc cgagcccgtc gtccatgaac gggaggtcgc cgaggtcgac ggccagggtc
     8581 agtgtcggaa tgcgccggaa caaggcggcg aacacgacct gcagctccag gcgggccagc
     8641 ggttggccgg tgcacttgtg cacgccgaac ccgaaggcga gctgctggct ggcatcgcgg
     8701 cgcagatcca gcactccggc agcggggaac acgctttcgt cacggttgcc gatctcctcc
     8761 gggaggatca gaccctcgcc ggcgcggatc acctgcccgg cgatctcgat gtcctccacg
     8821 gccacgcggc ggcggccggg ctggacggtg gtcaggtatc gcagcaactc gtcgacggcc
     8881 gcggcgactt gggggtcgtc ggaatccttc agcacagtga tctggtcggg atgctgcagc
     8941 agggccagtg tgcccagtgc gatcatgctg gccgtggtct cgtgcccgga gatcagcagg
     9001 aaaaggacca gcatcaccag ctgatgccgg ttcagctggc cggtggacag ccgttcggtg
     9061 gccagttggg acagcaggtc gtcctcgggg gcggcgagct tgcggccgat ctgcttggcc
     9121 aggtagtggt acagctcgcg ctcggacttg ctcacgacgt cgtccggggc gtcgacgctg
     9181 gtcatcgtcc tgatggcgtg gtggaagaag tcgtggtcct cgtaggggac gcccagtacg
     9241 tggcagatgg tgatggacgg aatcggcagg gcgaaatccg acacgaaatc cgccggcttg
     9301 gggccggcca ggagggcgtc gatgtggtcg tcgacgactt gctggaccgt gggccgtagt
     9361 tgctcgacct tgcggggggt gaacggccgg gcgatcatcc ggcgcaaccg gaggtgctcg
     9421 gggccttcca tgacggtcag gaactggccc tcttcatggt gttcctgcag gaactgactg
     9481 tggaacgggt agcccggctg catccagtcc gcgctgaccc ggcggtccga caggagcgcg
     9541 cactgctccg catgcctcgt caccagccag ggagtgctgc cgtcccacag ccgaactcgg
     9601 accagcggcg cgcgttcctg cagctcggcg agctgcggtg ccgggtccag cggacaacca
     9661 ggcgccctgg gcatcggaaa gtacgggact gccgtgggtt cctgatgagg aacgttggcc
     9721 tgcgcagacg tcacaatcgt agctccgtcc cgaatgtcac atgatggaag gcgcgtagaa
     9781 atagaggccg atggcactca gcccttcagg ctgatgcagg aaccatgggc cgggtgttcg
     9841 ccgggagacg atccctccat gcggagaaat ctcatgaccg ccgacgcgga gatactgccc
     9901 aaaaactcga atgattgcgc tcgttgaagg gagtgaccgc cgagccgact tccgcgatct
     9961 gcgtattccg gggccttgat cttttctgtt acgtggcggg aacctattca tgttcagccc
    10021 tcgtccgccg ggggtgagca gcatcagacg gcgagctctc cgcctggtgc tgttccggac
    10081 ccgaagcagc ctacccagac tcgatcaagc ccgacaagag gccggaaagg cccggcgtcc
    10141 gttggtcggc ggccgtcggg caagtgcgcg atgcgggaga ttgtgtacgc gagacgctgc
    10201 cggaacggct cgggccgtcg gcagttcccg ccccatgaaa tggctcggcc ggagcggtga
    10261 agtgctgggc cttcttgatt cgtccggcta gattcctccc gatcaaccaa acccgctgtc
    10321 atcgcgtccg tcacggagag agaggctctc accaatgaat cggccctccg tgcgcgaccg
    10381 gatttggatc atcgaaacgt gccggggcct cgacccgcgg aatggggaga ttaggtgtgg
    10441 gaagcgctct cgtgatcttg cgttgtggca gaccgaagag cttccatcgt cgcaggccgc
    10501 agggcacttt cttattcccg gcaagatcac ttccacgtat cggccgatgg atccgggatc
    10561 ggatgtgacg gcggaaaacg tgtgcactcc ccattgacga gacgccaaag actgtatgag
    10621 ataaggatgt tggagccagt caaggcgaag aggggtcaaa aatggcacag gaactgcagg
    10681 agcgttcgct gaccgtgacg gacgaggcgg agccggacat ggtgctcttc tccgacgagg
    10741 aaggtcttga tcttgccgat gacgtcgaat tgatcacgct gtcatcggcc actttgggtg
    10801 ccatcgtcgc gcggcgctgc tgatcgcatc tgatgccgac tcagaagcga ctggccctct
    10861 cgtttgccgg ggagggccag gtgtcggtcc gatcgtccac gcggcaggct cgcgtgacgc
    10921 tgggccgccc cttgatcaat tccgatgtca attctccttt cttcgttcct ctcgggctgc
    10981 tctgtatcgc ctctccgctg atggctcacg gccatcccgc agtcgtcgtc gacctggagt
    11041 acgagcaccg gattggcgcc ttcgagcccg gtgacgcgga ctggctcgac cagacgtgcg
    11101 ctcgcttcct ggccgccgat cccgacattc tcggcctcac ctgcctcgcg gacaccctcc
    11161 cggcatgcct gctcatcggg cgtgaggtca agcggctccg acccgatgtc acggtggttc
    11221 tcggcggacc cggaacgttc ggtgtcttcc ccgacgctct cccgcgcttc gccgactgtg
    11281 tcgacttcgt gtgccgcggc gagggagagc tcgcgatcac cgccctcgcg gatgccgttg
    11341 ccgacggggc cgatcccgcc ggagtgcccg gcttctggtc gtgcaggaac agccaggccg
    11401 tctccagtgg gcctcagccg accacgaacc tgaacctcgt gccgatgccc gcgtacggcg
    11461 ccgtcccgat gggcgactac atccgcctgt cgaccccgca catcttcgac gtgcacatcg
    11521 gctccgggtg cacctattcg tgcaagttct gcatgacgtc gacgttctgg gaccgtgact
    11581 tccggatcaa ggaccccgcg gtcatcctcg aagagcttcg cttcctgcac cgcacgtacg
    11641 gggtgaccca gttcaacttc ctgcacgaca acttcgccaa caagcgcacg tacctggacg
    11701 agttcgtgtc ctatttcgcc gagcacaacg acatgttcga gtggggctgc gcggtccgac
    11761 cggacaacgt caacccgtcc cttctgcggc gcatggccga cgccggatgc ttcaacatct
    11821 tctgcggcac ggacgccggg tcggagaaga tcctcaaggc catgctgaag atgccctcga
    11881 cgaagcggac ctacgagttc tttcggtccg cccaggaagt cggcat
//
