LOCUS       NZ_CP070373            11938 bp    DNA     linear   CON 18-MAY-2024
DEFINITION  Streptomyces sp. MBT27 chromosome, complete genome.
ACCESSION   NZ_CP070373
VERSION     NZ_CP070373.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. MBT27
  ORGANISM  Streptomyces sp. MBT27
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S.S., Hankemeier,T. and van
            Wezel,G.P.
  TITLE     Discovery of novel glycerolated quinazolinones from Streptomyces sp.
            MBT27
  JOURNAL   J Ind Microbiol Biotechnol 46 (3-4), 483-492 (2019)
   PUBMED   30729343
REFERENCE   2  (bases 1 to 8400223)
  AUTHORS   Machushynets,N., Elsayed,S., Carrion,V.J., Siegler,M.A., de la
            Cruz,M., Genilloud,O., Hankemeier,T. and van Wezel,G.P.
  TITLE     New from old: conjugation of actinomycin X2 and anthranilamide to
            form the novel actinomycin L in Streptomyces sp. MBT27
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8400223)
  AUTHORS   Du,C., Machushynets,N. and van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2021) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   4  (bases 1 to 8400223)
  AUTHORS   Machushynets,N.V., Wu,C., Elsayed,S., Hankemeier,T. and Van
            Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2020) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 1.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011751035.2-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 03:23:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,575
            CDSs (total)                      :: 7,482
            Genes (coding)                    :: 7,345
            CDSs (with protein)               :: 7,345
            Genes (RNA)                       :: 93
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 39 of 137
            Pseudo Genes (incomplete)         :: 114 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 27 of 137
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:14:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3406036
            Orig. end                         :: 3417974
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MBT, Institute of Biology
            Leiden, Leiden University.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11938
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11938
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11938
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6938](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6938
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            273..821
                     /locus_tag="GWQ54_RS15015"
     CDS             273..821
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /locus_tag="GWQ54_RS15015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_167162083.1"
                     /transl_table=11
                     /translation="MRITHIATTAAAAALTLTGTTAAFGAASPSATADAVFLQAIHQAN
                     LAEIAAGKDAGTHASSSCVKQVSAIIVRDHTKLDAKGAALAKTKNITLPKEPTAAQQKS
                     LSALKPMNGTAAYDTAWLKVQSSGHQQALALIDKELKSGNDAQIQAAAKEARPIVAAHL
                     KMVEGGKCHAMASSSPSPQ"
     misc_feature    1016..1029
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.48"
                     /tool="antismash"
     misc_feature    complement(1519..1533)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.19"
                     /tool="antismash"
     gene            1604..2917
                     /locus_tag="GWQ54_RS15020"
     CDS             1604..2917
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088740.1"
                     /locus_tag="GWQ54_RS15020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_167162081.1"
                     /transl_table=11
                     /translation="MAPGTTAAERRAARRPVPRDVRLFWWASASDALGTQASGVVLPLL
                     LLGLGHSAATVGLVAGVSTAAGLLLAPLAAVPADRGSRKAVMFWSATVSALAMAGVTAA
                     VATGQPPLALVLGAVLVERLSTAVYEAAAAGTVAMIAPPADCPRVVAGLAAGDQGALIL
                     GPALGGALYQLARALPFLADAVSYAVAAGCVRALRSDLRPARHPAPGTAPNGSVQAAEA
                     IAGAPSTPVPDKGRPGLRSELGAGLALVRTSPFLRLVVTWTATVNGVIVALYYGAVFTL
                     QKGGHGGAPMGLVLALSGAAGLVGSLAAPRVVGRIGAARLLVTVSWLLVPLAAGLATAD
                     GPWTYGALFGVFCLLMPLVAVVLQSRALQETPPSLQARTGAVLASAVGGSAALAPVVAG
                     VFVTRVGTAAPAVICAGLLTALAWRTTVVRSRLTGATA"
     gene            2914..4791
                     /locus_tag="GWQ54_RS15025"
     CDS             2914..4791
                     /NRPS_PKS="Domain: Abhydrolase_1 (351-496). E-value:
                     1.5e-10. Score: 33.1. Matches aSDomain:
                     nrpspksdomains_GWQ54_RS15025_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129145.1"
                     /locus_tag="GWQ54_RS15025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_167162079.1"
                     /sec_met_domain="PF00561 (E-value: 1.3e-07, bitscore: 30.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 1.5e-35, bitscore:
                     121.4, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRSYQVYRAALPEVCAAQPAKMVFTADADGRCEVFTWDADTRRA
                     RQVTDRPHGTLHGAIDRDARVWWFDEDDRGAGRWRFQPFEGGPDLPGLLGVPEGEPRGL
                     AVSDGGRVAMGIGDARSTTVYLGPRGGAAREVTRVDGHATLSGITPDGRLLALGGAAGS
                     ARAVTVIADDGTPCAVLAGDGGGGRLWSLGFAPTARHAELLLVRESNDRYLLASWTPDA
                     GLVTHSWCAFDTEITARWHPEGLSFLVRQDRHGRSTLHRVHLAERTVEPVPTPRGTLLD
                     AAPRAGSDVHVLWTDTATPPRMVSTAGTELPPTHGIPERVPGEHRDLWTPGPDGPVHSL
                     LSLPEGRTGAVPAVFLVHGGPADHDRDAYDGVVHSLVASGFAVVRVNYRGSTGYGPRWR
                     RAFGEGVGLTQVADLVAVRADLVARGVVRADAAGLWGTSWGGYLVLLALGTHPDLWQAG
                     VAVKPVADCATAYRTTTPALRALDERLFGGTPDEVPERYERSSPRHYAAAVHAPLLVAA
                     ATRDAKCPPGQVRDYLTALQLVGARHESMWLDTGHDGYEADDHVAVLRRAVVFLDRELR
                     QGQARTTTPQTPRSDRSGDSGRERSPAPAGTGGTHTERSSGHAEGHHPQ"
     misc_feature    3523..3525
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        3967..4401
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GWQ54_RS15025_Abhydrolase_1.1"
                     /evalue="1.50E-10"
                     /label="GWQ54_RS15025_Abhydrolase_1.1"
                     /locus_tag="GWQ54_RS15025"
                     /protein_end="496"
                     /protein_start="351"
                     /score="33.1"
                     /tool="antismash"
                     /translation="PAVFLVHGGPADHDRDAYDGVVHSLVASGFAVVRVNYRGSTGYGP
                     RWRRAFGEGVGLTQVADLVAVRADLVARGVVRADAAGLWGTSWGGYLVLLALGTHPDLW
                     QAGVAVKPVADCATAYRTTTPALRALDERLFGGTPDEVPER"
     misc_feature    4824..4844
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.21"
                     /tool="antismash"
     gene            5001..6938
                     /locus_tag="GWQ54_RS15030"
     CDS             5001..6938
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993375.1"
                     /locus_tag="GWQ54_RS15030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_167162078.1"
                     /sec_met_domain="TIGR03975 (E-value: 8.2e-185, bitscore:
                     614.4, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILKRSVDERVPGASAEVLHANLEF
                     TDWITERTEFTSDDYEYYALSSYFMGCGDWVFSSALYDDPAWRDEEFAAAMKGKLRKSR
                     LRMSQELHRQVPEFVEMIAQRIVEYAPDVVGFTSTFQQNTAALAAAKHVKRLAPHIVTV
                     MGGANCDGEQGEAVHRNFPFVDHVIRGEGESAFPQLLTAIAEGAGDEVLSKVPGLCHRD
                     PEGRSVVNAMATSPLPPATILAPDYSGYFERLATSVARNWVEPKLVVEGARGCWWGEKH
                     HCTFCGLNGSFMQFRSKSPDTFFEEIMELARRHRVLDMYLVDNILDMGYLTTVLPRIME
                     SGYDLRMHIEIKANMRRSQLRTLAEAGLIYVQPGIESLNNRVLDLMDKGVSGCQNVRML
                     RDGAETGLSVAWNYLHGFPGETAEDYDPVIAQIPALEHLNPPVDLSARIAIERFSPYFN
                     RPELGFDGLRPEEHYRFTYDLPESELYDLAYVFEAPQRGITEPTVTALNDALDAWKKHH
                     ADARLTHTDLGDRIVLVSRRRTFAWRAMEVTDPFELAVFRLLDQPHAVTALTRKAVARA
                     KGAERTEHEVQQLLDSWLALGLVFTDGGQYVHIAPSAVNEDLLRLDFMRHAQAALDADE
                     QPARPEPVSV"
     gene            6976..7653
                     /locus_tag="GWQ54_RS15035"
     CDS             6976..7653
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958122.1"
                     /locus_tag="GWQ54_RS15035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5825 family protein"
                     /protein_id="WP_167162075.1"
                     /transl_table=11
                     /translation="MSTALTTATEALGVAAWRDYDPEACTLPGMYLGEMELTGPPAEES
                     DRLWKLGARRVRLPEPVDLADLAAPGAAERAVRALSLVRDLTARAVLVEWKLRLDPAAP
                     DDTWRTLSHLQPPQRIEGPASATADLRRWRDGHYLCKCLWRQGPGFVQIRDRRWGDLRR
                     FTAEEPEYHRTIEALAYGAPAASVPAAVLADFLGERLVLAVGELAWWMPYRVNRWIQEA
                     MAI"
     gene            complement(7737..8846)
                     /locus_tag="GWQ54_RS15040"
     CDS             complement(7737..8846)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381199.1"
                     /locus_tag="GWQ54_RS15040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="WP_167162073.1"
                     /transl_table=11
                     /translation="MADTSHTPSSYWSRLRGRTADPAARETAGATPNVEPAGHTASPAG
                     SDDEPSAPTDLPARSWLAVLRRTGREFLDDDLPDKAAALTYYGVLSLFPALLVLVSTLG
                     VMGEKATRGILDNLGHLTPGPVHDLLDSAIRQLQGNGGTSSVVAVVSLIAALWSASSYV
                     AAFIRAANAVYDLPEGRPVWKLTPLRLALTVTLMVVLAVSAVIVVFTGPLAQRAGDAIG
                     AGDAAVATWNIAKWPVLLLLMVFMVALLFWRAPNVRGPGLRWLSPGSLLAVLLWLAASG
                     GFALYVANFGSYNKTYGTLAGLVIFLIWVWLSNLAILLGLELDAELARQRAISGGHPEG
                     EEPYVEPRDTRKWPARLRRRVRRRRNTAK"
     gene            9047..11110
                     /locus_tag="GWQ54_RS15045"
     CDS             9047..11110
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518552.1"
                     /locus_tag="GWQ54_RS15045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="regulator"
                     /protein_id="WP_167162071.1"
                     /transl_table=11
                     /translation="MAGKIPDETSSFVGRREELRRVERALSVHRLVTLTGTGGVGKTRV
                     ALRAAARGGPGHRDGVIWAELWPLMGSDLLIATVADAAGLADHTPRLAADALCAWLAER
                     EALLVLDSCEHLAADCRRLIGQLLRSAPGLTVLATSRQPLEVDGERVLAIGPLPVDGTR
                     GARAPSDAARLLLSRAAEAAPDVDVDSPRERAAADAICRRLEGIPLALELASGQLRQRR
                     LTDLAGALGSPLTMLSGEPSARPPRHASLRTTIGWSHELCTPAERLLWARLSVLRGPFD
                     ARTAQLVCYGGPLSKDGVADALSGLVVKCVVQRDGDRHRMLDSLKEYGRMWLEELGERE
                     QLADRHAAYFHEVVRRADERWLGPEQAREYGRIAQAHPDLCAALDHLMNDPGGAAVDLA
                     GRVAFFWSCCGHLHEARHYLERALLEHREPGPVLTRALWALGIVRLLQGEPEPAAQLAA
                     HCARAAEEDEDAEAALGAAYLMSLTYLMTGRPLVAQSLAESALAEAAEDDFVSPSRLRC
                     RLITVFTRTALGVYDEAEGLARRLLAACEQRDEVWTRSYVEYQLSLIALFRDRPAEAAE
                     HARAMLRGKRRLGDRFGIALGVDLLAAAAAEQGEGEQAARLSGTGQTYWRSVGHPQRGT
                     PELAAVRERTDLRARTAIGDHAYRVARQRGASDTRATLALALGPAEPPAPPE"
     gene            complement(11136..11543)
                     /locus_tag="GWQ54_RS15050"
     CDS             complement(11136..11543)
                     /EC_number="3.5.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949595.1"
                     /locus_tag="GWQ54_RS15050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RidA family protein"
                     /protein_id="WP_167162069.1"
                     /transl_table=11
                     /translation="MTITLVNPEGLPEVGVYHQVSVASGSKLVFLAGQVSVDADATTLG
                     EGDLAAQTERCYLNVATALAGAGATFADVAKLTVYVVDWAPEKMPLLLDGIARASAALG
                     TAIAPPGTLLGVAALDSPAHLVEVEAVAVLD"
ORIGIN
        1 gcagctcctc gccgcccata cgagccacct cgcccggcgc cccaaggcgg ccccctaccc
       61 ggcaccctga ccccacggcg gggccttcgt ccgtcgatcc acgggcgaat cgcggggccc
      121 tccgcccgat ggttacgggg agtgatctgg agaggcgcgc agtgaggagc acacctagga
      181 tgacgagggt cgggccctgc ccggactggc gttgagcgcg gtccggatgc cgaagcggcc
      241 cctacccgat ccacggacac gtggaggtac ccatgcgcat cacccacatc gccacgacgg
      301 ccgccgccgc ggcgctgacc ctcaccggaa ccacggccgc cttcggtgcg gccagcccct
      361 ccgccactgc ggacgcggtg ttcctgcagg ccatccacca ggcgaacctg gccgagatag
      421 ccgcgggcaa ggacgccggt acgcatgcga gcagcagctg tgtgaaacag gtctcggcca
      481 tcatcgtgcg cgaccacacc aagctcgacg ccaagggcgc ggctctcgcc aagaccaaga
      541 acatcaccct gccgaaggag cccaccgcgg cgcagcagaa gtcgctgtcc gcgctgaagc
      601 cgatgaacgg caccgcggcc tacgacaccg cctggctcaa ggtccagagc agcggccacc
      661 agcaggcgct cgcgctgatc gacaaggagc tgaagtccgg taacgacgcc cagattcagg
      721 cggccgccaa ggaggcccgc ccgatcgtcg ccgcgcacct gaaaatggtg gagggcggca
      781 agtgccacgc catggcgagc agcagcccgt ccccgcagtg acgctgctgc cggcatccgt
      841 tcccgcgccc cctcgccccc acgggtgagg gggcgcgggc gtgcgggccc ggtgcttcgc
      901 tcaacccggc ttcgcgcagc ggtcgttgag ccgggctgcc gggacgccct ctgatcgctc
      961 aactgcccct gcgatgagca gagttgatca atcaggggtc tctttcgagc ttcgaagaac
     1021 accggttcga gctttgaaga acgccgggct ccggtcgccg ggcgcgctcg tttccggcca
     1081 atgagtccga cggatggaac cgttccgcgc cggggtcgcc gcggggtggg cgggggtgtg
     1141 atgccgcccg ggggagccgg atgcggcgga cccgccgatc gtgacgcgcc cggggcgcgg
     1201 tggtggtcac cggttcgccg ccggaaaggt gcatatgccg gtcggagtgc gcgaactcgc
     1261 caccagggca cggaggttca ccgccgcgaa gcggacgtgg agggagcgcg aaggcatccg
     1321 ggagcgcgac gggcggccgc actctttccc tttcgtccct ttggatgccg acccgcgggc
     1381 gggccgaacc gcggatcaag taatcggtta ctttgccggg cccttacaca gttcgattcc
     1441 ggcctcggcg gcgcgactct tgtgggccga gcgggcgtct gattaggctc ccgctcgttt
     1501 cgattcctgc acatgttctt cgcaacttga ccaactgtgg ggtggctgtg cgcgaaccgg
     1561 agtttccggg tctcccgggg acctcttcag gggcgggaac gtgatggccc ccggcaccac
     1621 cgccgccgag cgacgggcgg cccgccggcc ggtgccccgt gacgtccggc tgttctggtg
     1681 ggcgagcgcc tccgacgccc tgggcaccca ggcctccggt gtcgtactgc cgttgctgct
     1741 gctcggcctc ggccattccg ccgcgacggt cggcctcgtc gcgggggtgt ccacggcggc
     1801 cgggctgctg ctggccccgc tggcggccgt tccggccgac cgggggtccc gcaaggccgt
     1861 gatgttctgg tccgcgacgg tctcggcgct cgccatggcc ggggtgacgg cggcggtggc
     1921 cacggggcag ccaccgctcg ccctcgtgct cggcgcggtc ctcgtggagc ggctctcgac
     1981 cgcggtgtac gaggccgccg cggccggcac cgtggccatg atcgccccgc ccgccgactg
     2041 cccgcgcgtg gtggccgggc tggcggcggg cgaccagggc gcgctgatcc tgggcccggc
     2101 cctcggcggt gcgctctacc agctggcccg cgccctgccc ttcctcgcgg acgccgtctc
     2161 ctacgccgtc gccgccggat gcgtacgggc gctgcgctcg gacctgcggc ccgcgcgcca
     2221 cccggccccc gggaccgcgc cgaacggctc ggtgcaggcg gccgaagcga tcgccggagc
     2281 tccctcaact cccgtacctg acaagggacg tccgggactg cggagcgagc tcggcgcagg
     2341 actcgccctc gttcgcacct cgcccttcct ccggctcgtg gtgacctgga ccgccaccgt
     2401 gaacggggtg atcgtcgcgc tctactacgg cgcggtgttc accttgcaga agggcggtca
     2461 cggcggcgcg cccatgggcc tcgtcctggc gctgtccggc gccgctggcc tcgtcgggtc
     2521 gctggccgcg ccgcgtgtcg tggggcgcat cggcgccgcg cggctgctgg tgacggtgtc
     2581 ctggctgctc gtcccgctcg cggccggcct cgccaccgcc gacggaccct ggacgtacgg
     2641 cgcgttgttc ggggtgttct gcctcctgat gccattggtc gcggtcgtgc tccagtcccg
     2701 cgccctgcag gagacgccgc cctccctgca ggcccgcacc ggcgcggtcc tcgcgagcgc
     2761 cgtcggtggg tcggccgcgc tcgcgccggt cgtggccggg gtgttcgtga cccgcgtcgg
     2821 gacggccgcg cccgccgtga tctgcgcagg actgctcacc gccctcgcct ggcggaccac
     2881 cgtcgtacgg tcccggctca cgggggcgac ggcatgagca ggagctacca ggtctaccgc
     2941 gcggccctgc ccgaggtgtg tgccgcccag cccgccaaga tggtgttcac cgccgacgcc
     3001 gacggccgtt gcgaagtgtt cacctgggac gcggacaccc gccgggcccg acaggtcacc
     3061 gaccgcccgc acggcaccct gcacggcgcg atcgaccgcg acgcccgggt gtggtggttc
     3121 gacgaggacg accgcggagc cgggcgctgg cggttccagc cgttcgaggg cggccccgac
     3181 ctgccgggtc tgctcggcgt gcccgagggc gagccgcgcg gcctcgcggt gagcgacggc
     3241 ggacgcgtgg ccatgggcat cggcgacgcc cgctccacca ccgtgtacct cggcccgcgt
     3301 ggcggtgccg cccgcgaagt gacgcgcgtc gacgggcacg ccaccctgtc cgggatcacc
     3361 cccgacggcc gcctcctcgc cctcggcggc gcggccggat cggcacgggc cgtgaccgta
     3421 atcgccgacg acggcacccc gtgcgcggtg ctcgccgggg acggaggcgg gggccggctc
     3481 tggtcgctgg gcttcgcgcc caccgcgcga catgccgaac tcttactcgt ccgggagtcc
     3541 aacgaccgct acctgctggc gagttggacc ccggacgccg gactcgtcac ccactcctgg
     3601 tgcgccttcg acaccgagat caccgctcgc tggcaccccg aaggactctc gttcctcgtc
     3661 cgccaggacc ggcacggccg cagcaccctg caccgcgtac acctcgcgga gcgcaccgtc
     3721 gagcccgtac cgacaccgcg cgggaccctc ctcgatgcgg cgccgcgtgc cgggtccgat
     3781 gtgcacgtcc tctggacgga caccgcgaca ccgccccgca tggtctccac cgccggcacc
     3841 gagttgcccc cgacgcacgg cattcccgag cgggtgcccg gcgagcaccg ggacctgtgg
     3901 acacccggcc ccgacgggcc cgtgcactcg ctgctcagcc tgcccgaggg acggacgggc
     3961 gcggttcccg ccgtcttcct cgtgcacggc ggtcccgccg accacgaccg ggacgcctac
     4021 gacggcgtcg tgcactccct cgtcgcctcc ggattcgccg tcgtacgcgt caactaccgc
     4081 ggctccaccg gctacggacc ccgctggcgg cgggcgttcg gcgagggcgt cggactcacc
     4141 caggtcgccg acctcgtcgc ggtccgcgcc gacctcgtgg cccgcggcgt ggtccgcgcg
     4201 gacgcggccg ggctgtgggg cacctcctgg ggcggctatc tggtgctgct cgccctcggc
     4261 acacaccccg atctgtggca ggcgggcgtc gccgtcaagc ccgtcgccga ctgcgcgacc
     4321 gcctaccgca ccaccacccc ggcgctgcgc gccctggacg agcgcctttt cggcggcacc
     4381 cccgacgagg tgcccgagcg ctacgagcgc agctcgcccc gccactacgc cgccgccgtc
     4441 cacgcacccc tgctggtcgc cgccgcgacc cgggacgcca aatgcccgcc cggccaggtg
     4501 cgcgactacc tcaccgccct tcaactcgtc ggcgcacgcc acgagtcgat gtggctcgac
     4561 accggccacg acggctacga ggccgacgac catgtggccg tgctgcgccg cgccgtggtc
     4621 ttcctcgacc gcgaactgcg gcaggggcag gcccgtacca ccacccccca gacgccccgc
     4681 tccgaccgga gcggggattc cggacgggag cgctccccag cgcccgccgg aacaggcggt
     4741 acccacacag agaggagttc cggccatgca gaaggacatc atccacaatg acccgctcgc
     4801 gggtgacgag gaaaaccgca agccgagcgt cggcgtgacc atcaaggttc cgttccgcaa
     4861 cgccgaggac ggcgaagagg actgatcctc gccggtcggt cccgcggccg tgagccgcgg
     4921 gaccgaccgg gcactaccgc ccaactccgg ccccccgggc ctcgttccct ccacgtctcc
     4981 gcacatcccg aggagccgac atgcgtgttc tgctggtcaa tatgccctgg tcgcccatcg
     5041 accttccctc gctggccctg ggaatcctca agcgcagcgt ggacgagcgc gtgcccggcg
     5101 ccagtgccga agtcctgcac gccaacctcg aattcaccga ctggatcacc gagcgcaccg
     5161 aattcacctc cgacgactac gagtactacg cgctctcctc ctacttcatg ggctgcggcg
     5221 actgggtgtt ctcctccgcg ctctacgacg accccgcctg gcgggacgag gagttcgcgg
     5281 ccgcgatgaa gggaaaactc cgcaagtcgc gactgcggat gtcgcaggaa ctccaccgcc
     5341 aggtaccgga gttcgtggag atgatcgccc agcgcatcgt cgagtacgcg cccgacgtcg
     5401 tcggcttcac ctccaccttc cagcagaaca ccgcggccct cgcggccgcc aagcacgtca
     5461 agcggctcgc cccgcacatc gtgaccgtga tgggcggcgc caactgcgac ggcgagcagg
     5521 gcgaggccgt ccaccgcaac ttcccgttcg tggaccacgt gatccgcggc gagggcgaat
     5581 ccgcgttccc gcagctgctg accgcgatcg ccgagggtgc cggcgacgag gtgctctcga
     5641 aggtgccggg cctgtgccac cgcgatcccg agggccggag cgtcgtcaac gcgatggcca
     5701 cgagcccgct gccgccggcc accatcctgg cccccgacta cagcggctac ttcgagcgcc
     5761 tcgccacctc cgtggcccgc aactgggtcg agcccaagct cgtcgtcgag ggcgcgcgcg
     5821 gctgctggtg gggcgagaag caccactgca cgttctgcgg cctcaacggc tcgttcatgc
     5881 agttccgctc caagagcccg gacacgttct tcgaagagat catggaactc gcccgccgcc
     5941 accgggtgct cgacatgtac ctcgtcgaca acatcctcga catgggatac ctcaccacgg
     6001 tgctgccccg catcatggag agcggctacg acctgcggat gcacatcgag atcaaggcca
     6061 acatgcggcg cagccagctg cgtacgctcg ccgaagccgg actcatctac gtacagcccg
     6121 gcatcgagag cctcaacaac cgggtgctcg acctgatgga caagggcgtc agcggctgcc
     6181 agaacgtgcg catgctccgc gacggcgccg agaccggcct ctccgtcgcc tggaactatc
     6241 tgcacggctt cccgggcgag accgccgagg actacgaccc cgtcatcgcc cagatccccg
     6301 cactcgaaca cctcaaccct ccggtcgacc tctcggcgcg gatcgccatc gagcgcttca
     6361 gcccctactt caaccgcccc gaactcggct tcgacggcct gcgtcccgag gagcactacc
     6421 gcttcaccta cgacctgccc gagtccgagc tctacgacct cgcgtacgtc ttcgaggccc
     6481 cccagcgcgg catcaccgag cccacggtca ccgcgctcaa cgacgcgctc gacgcctgga
     6541 agaagcacca cgccgacgcc cggctcaccc acaccgacct cggcgaccgc atcgtcctgg
     6601 tgagcaggcg gcgcaccttc gcctggcgcg cgatggaagt gaccgacccg ttcgaactgg
     6661 cggtcttccg cctcctcgac cagccgcacg cggtcaccgc gctcacccgc aaggcggtag
     6721 cccgcgccaa gggcgccgag cgcacggagc acgaggtgca gcaactgctc gactcctggc
     6781 tcgcgttggg cctggtcttc acggacggcg gccagtacgt ccacatcgcg ccctcggccg
     6841 tcaacgagga cctgctgcgc ctcgacttca tgcgccatgc ccaggccgcc ctcgacgccg
     6901 acgagcagcc cgcgcggccc gagcccgtga gcgtctgagc ccgcgcgtgt ccgacacaga
     6961 agcaggagac gaccgatgag caccgccctc accaccgcga ccgaagccct cggggtcgcc
     7021 gcctggcgcg actacgaccc cgaggcctgc accctgccgg ggatgtacct cggcgagatg
     7081 gaactgaccg gccccccggc cgaggagagc gaccggctgt ggaagctggg cgcccggcgc
     7141 gtccggctgc ccgagcccgt cgacctcgcc gacctcgcgg cgccgggcgc ggccgaacgc
     7201 gccgtgcgcg ccctgagcct ggtccgcgac ctgacggcgc gtgccgtcct ggtcgaatgg
     7261 aagctccgcc tcgacccggc ggcgcccgac gacacctggc gcaccctcag tcacctccag
     7321 ccgccccagc gcatcgaggg gcccgcgagc gcgaccgcgg acctgcgccg gtggcgcgac
     7381 ggccactacc tctgcaagtg cctgtggcgc cagggcccgg gcttcgtcca gatccgcgac
     7441 cgacgctggg gcgacctgcg ccgcttcacg gccgaggagc cggagtacca ccggaccatc
     7501 gaagccctgg cctacggagc cccggccgca tccgtgcctg ccgccgtact ggccgacttc
     7561 ctcggcgagc gcctcgtcct ggcggtgggg gagctcgcct ggtggatgcc gtaccgggtg
     7621 aaccgctgga tccaggaggc catggcgatc tgaccggccg gggacgggcc gccgcgcgca
     7681 cggcgcggcg gccgctccgg cgtgcgaggc tcaatcaccg tgcgggaagc ggcacttcac
     7741 ttggcggtgt tgcgacgccg ccgcacccga cggcgcagcc gcgcgggcca cttgcgggtg
     7801 tcgcggggct ccacgtacgg ctcctcgccc tcggggtgac cgccgctgat cgcgcgctgg
     7861 cgggccagct cggcgtccag ttcgaggccg agcaggatcg ccaggttcga gagccacacc
     7921 cagatcagga agatgaccag cccggcgagc gttccgtacg tcttgttgta cgagccgaag
     7981 ttcgccacgt acagcgcgaa gccgcccgat gcggccagcc acagcaggac ggcgagcagg
     8041 ctgcccgggc tcagccaacg caggcccggg ccgcggacgt tgggggcgcg ccagaagagc
     8101 agggccacca tgaagaccat caggagcagg agcaccggcc acttcgcgat gttccaggtc
     8161 gcgaccgccg cgtcgcccgc cccgatcgcg tccccggcgc gctgggccag cgggcccgtg
     8221 aagaccacga tcaccgcgct gaccgcgagc accaccatga gcgtgaccgt cagggcgagc
     8281 cgcagtggcg tcagcttcca caccggccgg ccctcgggca ggtcgtagac ggcgttcgcg
     8341 gcgcggatga acgcggcgac ataactggac gccgaccaca gggccgcgat caggctgacc
     8401 accgcgacga cgctgctcgt accgccgttg ccctgcagct gccggatcgc gctgtcgagc
     8461 agatcgtgga cggggccggg cgtgaggtgg cccaggttgt ccaggattcc gcgcgtggcc
     8521 ttctcgccca tcacgccgag cgtcgagacg agcacgagca gtgcggggaa cagcgacagc
     8581 accccgtagt aggtcagggc ggccgccttg tcgggcaggt cgtcgtcgag gaactcccgg
     8641 ccggtacgcc gaagaaccgc aagccaggag cgggccggga ggtcggtggg cgcactgggc
     8701 tcgtcgtccg acccggccgg ggaggcggtg tggccggccg gttccacgtt cggtgtcgca
     8761 cccgccgttt cccgggccgc ggggtcggcc gtccggccgc ggagacggct ccagtacgaa
     8821 ctcggggtgt gcgaggtatc tgccatgcct ctcgggtccc ctcgaagtcg ccgctcacgc
     8881 gtccgcgcag gcggggactc cgttccggtg accggtgagc cggcgacacg tgcccgagcc
     8941 gagtccgccc gatggtgtga cgctcggcag cgggcaccgc acaccgtggg gtggtcccgg
     9001 gccgaacgcc gcacccgagt cgtgctcgtg ggccacactg tgcgccatgg cgggcaagat
     9061 tcctgacgag acgagcagct tcgtgggacg gcgggaggag ctgcgacgcg tcgagcgggc
     9121 cctctccgtc caccggctgg tgacgttgac cggaaccggc ggggtcggaa agacccgggt
     9181 cgccctacgg gccgccgcgc gcgggggccc cggccatcgc gacggggtca tctgggccga
     9241 gctgtggccc ctcatgggca gcgacctgct gatcgcgacg gtcgccgacg cggccggtct
     9301 cgccgaccac accccacggc tggccgccga cgcgctgtgc gcgtggctgg ccgagcgcga
     9361 ggcgctcctc gtcctcgact cgtgcgaaca cctcgcggcc gactgccgtc ggctcatcgg
     9421 tcaactcctg cgttccgcac cgggcttgac ggtcctggcc acgagccgac agcccctgga
     9481 ggtcgacggg gaacgcgtcc tcgccatcgg cccgctgccg gtcgacggaa cgcgcggggc
     9541 acgagcaccc agtgacgcgg cgcggctgct gctctcccgg gccgccgaag ccgcacccga
     9601 tgtggacgtg gactccccgc gcgagcgggc cgccgccgac gcgatctgcc gccgcctgga
     9661 aggcattccg ctcgccctgg agctggcgag cggccagttg cggcagcgcc gtctgacgga
     9721 tctcgccggg gcgctcggct cgcccctcac gatgctgagc ggcgagccga gcgcccggcc
     9781 accccggcac gcgagcctgc gcaccacgat cggctggagc cacgaactgt gcacccccgc
     9841 cgaacggctg ctgtgggcgc ggctctccgt actgcgtggc cccttcgacg cacgcacggc
     9901 ccaactggtc tgttacggtg gcccgttgag caaagacggc gtggctgacg ctctgtcagg
     9961 gctggtcgtc aaatgcgtcg tccagcgcga cggcgatcgc catcgcatgc tcgactccct
    10021 caaggagtac gggcgcatgt ggctggagga gctcggcgag cgcgaacagc tggcggaccg
    10081 gcacgccgcg tacttccacg aagtcgtccg gcgggccgac gagcgctggc tcggcccgga
    10141 acaggcccgg gagtacggga ggatcgcgca ggcgcacccc gacctgtgtg ccgcgctcga
    10201 ccacctgatg aacgaccccg gcggtgcggc ggtcgacctg gccgggcgcg tcgccttctt
    10261 ctggagctgc tgcggccatc tgcacgaggc ccgccactac ttggagcgcg cgctgctcga
    10321 acaccgggaa cccgggccgg tgctgacccg ggcgctgtgg gcgctcggca tcgtgcggct
    10381 gctccaaggg gagccggagc cggccgcgca gctggccgcc cactgcgcga gggcggcgga
    10441 ggaggacgag gacgccgaag cggcactggg ggccgcctac ttgatgagcc tcacctacct
    10501 gatgaccggt aggcccctgg tcgcccagag cctcgccgaa agcgccctcg ccgaggccgc
    10561 cgaggacgac ttcgtctccc cctcgcgcct ccgatgccgc ctgatcaccg tgttcacgcg
    10621 gaccgcgctg ggcgtctacg acgaggcgga gggcctcgcc cggcggctcc tcgccgcctg
    10681 cgagcagcgt gacgaggtct ggacgcgctc ctacgtggag taccagctgt ccttgatcgc
    10741 cctgttccgg gaccggcccg cggaggccgc ggagcacgca cgggcgatgc tgcggggcaa
    10801 gcgccggctc ggcgaccggt tcggcatcgc cctgggcgtg gatctgctgg cggcggccgc
    10861 cgccgagcag ggcgagggcg aacaggccgc ccggctctcc ggcaccgggc agacgtactg
    10921 gcgcagtgtc gggcatccgc agcgcggcac tccggaactg gccgcggtgc gtgaacgcac
    10981 cgatctgcgg gcccgtaccg cgatcggtga ccacgcctac cgggtggcgc gccagcgcgg
    11041 ggcgtccgac acccgcgcca ccctcgccct ggccctcggc ccggccgagc cccccgcccc
    11101 gcccgagtga tccggctcgg cccggccgag ccggatcagt cgaggaccgc caccgcttcg
    11161 acctcgacca ggtgggcggg cgagtccagg gccgcgacgc ccaggagcgt gcccggcggg
    11221 gcgatggccg tgccgagcgc ggcggacgcg cgggcgattc cgtcgaggag cagcggcatc
    11281 ttctccgggg cccagtcgac gacgtacacg gtcagcttcg ccacgtcggc gaacgtcgcg
    11341 cccgcccccg ccagcgcggt ggcgacattg aggtagcagc gctcggtctg tgcggcgaga
    11401 tcgccctcgc cgagcgtggt cgcgtcggcg tcgacggaca cctgcccggc gaggaagacg
    11461 agcttcgatc cgctcgcgac cgacacctgg tggtagacgc cgacctcggg cagtccttcg
    11521 gggttcacaa gggtgatggt catgccgccc gcttccgtgt gcgagagcgc acgggcccgc
    11581 tgccgcgtgg cccaactcgc cctcggtctc ttgtagttac tcgggaaccg taggagagtg
    11641 gccgctgaca tggaagaacg cactttccag tgactgggga acccgatggt gaccaagcag
    11701 ctcaggagca cggccgagga cgcggacctg agccgcgcgg actcgctggc ccgggagatc
    11761 ttcaccgaca tagccaacaa gtgggcgttc ctgatcatcg aggcgctcgg cgacggcacc
    11821 ctgcgcttcg gtgcgctgcg caacgagatc cagggcatca gccacaagat gctcacccag
    11881 aacctgcgca tgctggagcg caacggcctg gtcgagcgcg atgtgcaccc cacggtgc
//
