LOCUS       NZ_CP036534            10216 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. VN1 chromosome, complete genome.
ACCESSION   NZ_CP036534
VERSION     NZ_CP036534.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. VN1
  ORGANISM  Streptomyces sp. VN1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8341703)
  AUTHORS   Nguyen,H.T., Pokhrel,A.R., Nguyen,C.T., Pham,V.T.T., Dhakal,D.,
            Lim,H.N., Jung,H.J., Kim,T.S., Yamaguchi,T. and Sohng,J.K.
  TITLE     Streptomyces sp. VN1, a producer of diverse metabolites including
            non-natural furan-type anticancer compound
  JOURNAL   Sci Rep 10 (1), 1756 (2020)
   PUBMED   32019976
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8341703)
  AUTHORS   Pokhrel,A.R., Nguyen,H.T., Dhakal,D., Nguyen,C.T., Pham,V.T.T. and
            Sohng,J.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2019) Department of BT-Convergent Pharmaceutical
            Engineering, Institute of Biomolecule Reconstruction, Sunmoon-Ro221,
            Asan, Chungnam 333-708, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2018
            Assembly Method                   :: HGAP v. 3
            Assembly Name                     :: HGAP3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011650445.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 03:53:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,284
            CDSs (total)                      :: 7,197
            Genes (coding)                    :: 7,083
            CDSs (with protein)               :: 7,083
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 44 of 114
            Pseudo Genes (incomplete)         :: 82 of 114
            Pseudo Genes (internal stop)      :: 3 of 114
            Pseudo Genes (multiple problems)  :: 15 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:32:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7687325
            Orig. end                         :: 7697541
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036534.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jae Kyung Sohng.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="32"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1796..4018)
                     /locus_tag="EZV63_RS33760"
                     /old_locus_tag="EZV63_34230"
     CDS             complement(1796..4018)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="EZV63_RS33760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_34230"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_037766788.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.1e-39, bitscore:
                     133.1, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAQETRGRRRAAPPRRSVPKSGAKTVRTPTVLQMEAVECGAAS
                     LAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYGFTAKGMQMDLAALAEVAT
                     PAILFWEFNHYVVYDGMGRRFGRRGVFINDPGKGRRFVSLEDFDGSFTGVVLTMEPGAD
                     FTRGGRRPGVLGAMPARLRGTAGTLPAAVLASLLLVAVGAAVPALSRTYIDMFLIGGQT
                     SLLGVLFASMAACVLLTVLLTWLQQANLLHGRIISSTLSSARFLRHLLRLPVTFFSQRS
                     PADLVQRLQSNDAVAETLARDLAAAGVDAVVVVLYAVLLYTYDPQLTFVGVGVALLNIL
                     AMRVVVRLRATRTAKLRADTARLTNTAYTGLQLIETMKATGGEDGYFRKWAGQHATTLE
                     EQQRLGVPSAWLGVVAPTLATLNSGLILWIGGMRAVEGHISVGLLVAFQSLVVRFTAPL
                     TRLNGVAGRIQDFAADVARLKDVENFRTDPLYDRPGGPDSTRRLQGHVELQNVSFGYNP
                     LDTPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYAPWDGVIRVDGQRLEDVPR
                     GALAASVSFVDQDVFLFEGSVRDNVALWDPSIPDTAVVDALKDAALYDVVMRRPGGIGS
                     PVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELVVMDNLRRRGCACV
                     VIAHRLSTVRDSDEIVVLEHGSVVERGRHEQLVAHGGAYAALVRER"
     gene            complement(4015..4821)
                     /locus_tag="EZV63_RS33765"
                     /old_locus_tag="EZV63_34235"
     CDS             complement(4015..4821)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="EZV63_RS33765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_34235"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_070389904.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPDELDLPVRLARPQGWLVLSVTVVAMAAASVW
                     AVGGSVTSTVSAPAILTHGQGSYLLQSPVSGQVTAVLATPGQRLGADAPVLKVRTADGE
                     SVVRTVDAGRVSALAATVGQIIQTGANVASVEKVAHTGDPLYATVYVPADNAAAVPANA
                     SVDLTVQSVPTQQYGVLHGKVKSVDRSAQSAQTIGAFLGDSQLGEQFTKDGRPVAVTVR
                     LTASESTKSGYAWSSADGPPFELTSMTLASGSIRLADQRPVDWLLP"
     gene            complement(5001..5216)
                     /locus_tag="EZV63_RS33770"
                     /old_locus_tag="EZV63_34240"
     CDS             complement(5001..5216)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="EZV63_RS33770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_34240"
                     /product="hypothetical protein"
                     /protein_id="WP_031031870.1"
                     /sec_met_domain="strepbact (E-value: 3.6e-36, bitscore:
                     122.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSIPQVETTEISDADLDNVSGGLNVNAVGTVTGLVDGVAPVSGL
                     VNTAVGTVEGVTGLNTAPVTGLVAGL"
     gene            5826..7181
                     /locus_tag="EZV63_RS33775"
                     /old_locus_tag="EZV63_34245"
     CDS             5826..7181
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134775.1"
                     /locus_tag="EZV63_RS33775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_34245"
                     /product="S1 family peptidase"
                     /protein_id="WP_166625595.1"
                     /transl_table=11
                     /translation="MSHKRIPKRKAAMAAGGVVALGAAAILLPNANASQDGGGSHDAAA
                     APRTLKAADASDLASQLAGQLGDAFAGSYYDAGAQRLVVNVVPGDDDQVAVRAQRAGAE
                     IREVDNSMTELHGGAQTLKSEATIPGTSWAIDPRTNKILVTADSTVTGEKWDRLESTVG
                     RLGSGMATLEKSAGTFKTFVSGGDAIFGGGARCSLGFNVTAGDGSPAFLTAGHCGVAAD
                     AWSDAQDGQPIGTVDQAVFPGEGDFALVTYDDPATEAPSEVNLGDQTLQISGAAEATVG
                     EQVFRMGSTTGLADGQVLGLDATVNYPEGTVSGLIQTDVCAEPGDSGGSLFTQDGLAIG
                     LTSGGSGDCTAGGETFFQPVTTALEAVGATLGDGAGDGAGEQTGGEEAGGAGAGGDEPG
                     GAGGGDAGAGDEQPGGDDAGDSTGTVGAVSGSGSGTDDGTGTGAGHGSGPLG"
     gene            7539..9986
                     /locus_tag="EZV63_RS33780"
                     /old_locus_tag="EZV63_34250"
     CDS             7539..9986
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057834.1"
                     /locus_tag="EZV63_RS33780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_34250"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_070389906.1"
                     /transl_table=11
                     /translation="MVGEGAAERGSRTLRAEGALKAIAAGPDRQERTRRVLEQALVFAG
                     ASCAALYTPGDHRDLLCLVESAGVPRALYGVRDSYPASGGSPVADAHRGGAAVWVGPEE
                     FAGSAESRRVPSSGLSLAALPVRGDGGGCLLALTGHPDGFGAEDRACLELIAEALTCPV
                     PTEAAEGGELRPTAFTLAMDSGRVEVGDGVLRLFGLAPGEFDGRVETLLGLTVPEDLPS
                     LMSVTEADHMTIGERELEFRVLQPTGPPKWLRLSGRLLPGGEGRPTRLLGTVADASTLR
                     PDVTDVARVQRLAAALATAGTVRDVSQAVVAALRRPLRADRIALAELENERLVVTVLDP
                     PDPESWPELWRLEWRTEWPDAPVRAMPTLAAALREGRARIWPAGTGDLEPALADVGPGG
                     LAVLPLPAGGRMAGACLIGWDAPHDFGPDERALLTASAGLAGQALMRAHAFDAEHELVG
                     MLQRQLLPRRLPELPGAVAVARYLPSTAGLELGGDWYDVIPLPDHHVALVIGDVQGHSA
                     GAATLMGQMRTALRAYAAEGHPPDVVVSHANRLLVDMETDLFATCVYVDLDMEAGAAWC
                     VRAGHLPPVLRHPDGSTEIAEAEGGPPLGVVPQADFPMSPLRLRPGTLIALTTDGLVET
                     PESDIDAGLDRFAHALAGSDPAHLGLLADALLGEARRSDDVALLLVRYDGMALRPLRES
                     WSVWRLPEAVRHARRFARRTLRSWDVPGDTTDGVLLIVSELVTNALVHTDGQVRLDLAL
                     IGRRLRVAVADASPRTPAKPTSIGWEATGGRGILLVEAMSAAWGTLPVGGGKQVWSEIA
                     LEE"
     misc_feature    10118..10133
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.08"
                     /tool="antismash"
     misc_feature    complement(10121..10136)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.64"
                     /tool="antismash"
ORIGIN
        1 gaaccgttcc gcccacgccg cgtaggcgta gttctccgcc gccgccaccc gcagcttcgg
       61 gaggccgcgc agcgtctgga acgcctggtt gttcagcttg ttggtgagct tgaccagccg
      121 ccgctgccag cgcacctgcc acagcccgag gcccaggaag accccggcca cgaccaccag
      181 catgccgatc gccgccagcg ccatcggcac actgaaccag agcagcagcc ccaggttcat
      241 cgccccgacg gtcaccgact gggccaccgt cggaccgacg cccgccagca gccggcggat
      301 cgcgctgatg cccatcgccg cgctcgccag ctcgccggtc gagcgttcgg tgaagaactt
      361 cgtcggcagc ctcagcagcc ggtcccacac ggccggttgc agcgtcgcct cgacccggcc
      421 ctccaaccgc aggatggtga ggttctgcaa cagcatgaag gccgccgcca cgacaccgct
      481 cagcatcacc gcgagacaca cctgcacgat cagcccggtc tgcgccgtcg gcacgaactc
      541 gccgagcacc cggcccgtcg cgaccggcac cagcgcgccg agccccaccg tcaccaggcc
      601 gctcagcagc aggcccgtca gatcccggcg ggtgcccgcc aggcagaacc gcagcagccc
      661 gagcggaccg agccggccct ccggcagcgg ccggtagaac atcaccgcgc tcggctcgaa
      721 ctcctcggcg ttgtcccgct ccaccggcgt ctcccggccg gtcgccggat gcacggccgc
      781 atagccgccg cgccgccaca gcagcgccac cggcgccccc gacaggctcc ggtgcccgac
      841 cagcggcccc acgtcgtccc gccaccagcc gtcctccagc cgcaccgtgc gggtccgcag
      901 ccgcgaggcg agggcgaccc gctcgaccgg atcaagccgc tggctctccg tgccgctccg
      961 cgcgggctcg gcgagccgga ttccggcggc gcgggcgacc agccggcacg ccgcgtacga
     1021 ggcgtcggcg tcggcggccg tggtgcgccc cgccgaccgc ttcccgatgg aggccagcag
     1081 ggtccggtcg gcctgcgcgc gcacggcctc acccgccttg ataccggccg ccgtccgcgt
     1141 ctcgtgggtg cgctccaact gctcgatcca gcggtccagc gtcgtcagca gccggtactg
     1201 ctggtcgacc aggctctgcc acagcgccgg gtccatcagc aggtcggcgg cggcctcctc
     1261 gccgtacacc gccccgtact gcacgctgcc cggcggcacc tgcatccaga acacgtcgtc
     1321 gtcggtgggc gcggacgccc gttccgcggc catcggcgcc tggaacagga ccgtcaggcc
     1381 gcggcccacg ccgagcgcca gggcgtactc cagcgggctc gtcgtcggcg gcacgtactg
     1441 cgggttgccg tactcgtcgt acgaccacgt ctgcgtgggc gccggctggt acagctcgcg
     1501 cagcccgatg cggtgcacca cgcagtcccg cagcggacgc gcgaccaggg tgtgctgcgg
     1561 gcccgccacc ggaccgagca gcgccgagcc cgcctccagc cgtcccagat ggtgccagtg
     1621 gccctgctcc ccggcgtcga ccgcgaacag gtcgaccgcg ccggacgcca ccagccacag
     1681 cacctgcggc ccctccaggt cgaggcggtt gagcccggcg cagtccaccc gcgtgcccag
     1741 cgcgccgagc gccccgagga cgaggtcgcc ctgtccatcg tgcacggccg tcatctcacc
     1801 gctccctgac cagcgccgcg tacgcaccgc cgtgcgccac cagctgctcg tgccgtccgc
     1861 gctccacgac gctgccgtgc tccaggacga cgatctcgtc gctgtcccgc acggtgctca
     1921 gccggtgcgc gatcaccacg caggcgcagc cgcgccggcg caggttgtcc atgaccacca
     1981 gctccgtctc cgcgtccagc gcgctggtca cctcgtccag gaccaggatg ctggggcggc
     2041 gcaccagggc ccgcgcgatc tccaggcgct ggcgctgccc gccggagaag ttgcggccgt
     2101 cctgctcgac cgggctcccg atgccgccgg gacgccgcat cacgacgtcg tacagtgccg
     2161 cgtccttgag cgcgtccacc accgcggtgt ccggaatcga cgggtcccac agcgccacgt
     2221 tgtcgcgcac cgagccctcg aacaggaaca cgtcctggtc gacgaaggag accgaggcgg
     2281 ccagcgcccc gcgcggcacg tcctccagcc gctgcccgtc gacgcggatg accccgtccc
     2341 acggggcgta caggcccgag atcagccggg acaccgtgga cttgccgctg cccgagccgc
     2401 ccaccagcgc cacctgctgc ccggggccca cggtcaggtc gaagccggtc agcagcggtg
     2461 tgtcgagcgg gttgtagccg aaggagacgt tctgcagctc gacgtgcccc tgcagccggc
     2521 gggtggagtc cgggccgccg gggcggtcgt agagcgggtc cgtgcggaag ttctccacgt
     2581 ccttcagccg cgccacgtcc gccgcgaagt cctggatgcg gcccgccacg ccgttcaggc
     2641 gggtcagcgg ggccgtgaaa cggaccacca gcgactggaa ggcgaccagc agcccgaccg
     2701 agatgtgccc ctcgaccgcg cgcatgccgc cgatccacag gatcagcccg ctgttcagcg
     2761 tcgccagggt cggcgcgacc accccgagcc aggcactcgg cacccccagc cgctgctgct
     2821 cctccagcgt ggtggcgtgc tgccccgccc acttgcggaa gtagccgtcc tcgccgcccg
     2881 tcgccttcat cgtctcgatc agctgcaggc cggtgtacgc ggtgttcgtc agccgcgccg
     2941 tgtcggcccg cagcttcgcg gtacgggtcg cccgcagccg cacgacgacc cgcatcgcga
     3001 ggatgttgag cagcgccacg ccgaccccga cgaaggtcag ctgcgggtcg tacgtgtaca
     3061 gcagcaccgc gtacaggacc accacgaccg cgtccacgcc cgccgccgcg aggtcgcggg
     3121 ccagcgtctc ggcgacggcg tcgttggact gcaggcgctg caccaggtcg gccgggctgc
     3181 gctgcgagaa gaacgtcacc ggcagccgca gcagatgacg caggaagcgg gcgctggaga
     3241 gggtggagga gatgatgcgc ccgtgcagca ggttggcctg ctggagccag gtgagcagga
     3301 ccgtgagcag cacgcaggcc gccatcgagg cgaacagcac accgagcagc gaggtctgcc
     3361 cgccgatcag gaacatgtcg atgtaggtgc ggctgagcgc gggcaccgcc gcgccgaccg
     3421 ccaccaggag caggctggcc agcacggcgg cgggcagcgt cccggcggtg ccccgcagcc
     3481 gggccggcat cgcgccgagc acgccgggcc tgcggccgcc ccgggtgaag tccgcgcccg
     3541 gctccatggt caggacgacg ccggtgaagc tgccgtcgaa gtcctccagg gacacgaaac
     3601 gccggccctt gcccgggtcg ttgatgaaca ccccgcgccg cccgaaccgg cggcccatgc
     3661 cgtcgtagac gacgtagtgg ttgaactccc agaacaggat ggccggcgtc gccacctcgg
     3721 cgagcgcggc caggtccatc tgcatgccct tggcggtgaa cccgtaactg cgggcggcct
     3781 tcaggaggtt gctcgcccgg gaaccgtcgc gggagacgcc gcaggcgatc cgcagctcct
     3841 ccagcggaac gtgccggccg tagtggccga gcaccatcgc gagggaggcg gcgccgcact
     3901 cgacggcctc catctggagc acggtggggg tgcggacggt cttcgcgccg ctcttcggca
     3961 cgctgcgccg gggcggggcg gcgcggcgtc tgccgcgggt ctcctgggcg gtactcacgg
     4021 cagcagccaa tcgacgggac gctggtcggc caggcggatg gagcccgagg ccagggtcat
     4081 ggaggtcagt tcgaacggcg gcccgtccgc cgacgaccac gcgtagccgc tcttcgtcga
     4141 ctccgaggcc gtcagccgca ccgtcacggc caccggcctg ccgtccttgg tgaactgctc
     4201 gccgagctgg ctgtcaccga ggaacgcgcc gatcgtctgc gccgactgcg ccgagcggtc
     4261 caccgacttc accttcccgt gcaggacgcc gtactgctgg gtcggtaccg actgcacggt
     4321 caggtcgacg gaggcgttcg cgggtacggc ggcggcgttg tcggccggca cgtagacggt
     4381 ggcgtacagc gggtcgccgg tgtgggcgac cttctcgacc gaggcgacgt tcgcgccggt
     4441 ctggatgatc tggccgacgg tggcggcgag cgcgctgacc cggccggcgt cgaccgtgcg
     4501 gaccacgctc tcgccgtcgg cggtgcggac cttcagtacg ggggcgtcgg cgcccagccg
     4561 ctgtcccggc gtggcgagga ccgcggtgac ctgcccggag acggggctct gcaggaggta
     4621 actgccctgg ccgtgcgtga ggatggcggg cgcggagacg gtggaggtca ccgagccgcc
     4681 caccgcccac acggacgcgg cggccatcgc gaccacggtc acggacagca ccagccagcc
     4741 ctgcggacgg gcgagacgca ccggaagatc gagctcgtcc ggcgactgga gcttggagag
     4801 ggcctgttgg cggaactgca cggcggacgt accccagtga ttcccgtgat tccccgtgat
     4861 tcgccgtgct cccgaagagc ctcgtgagca cgagaggacc gggtgggacg agtgggttgg
     4921 cggaaggaag gcgtcgggtg gaacggccgg agagccccgg agcctgggcg gctccgggac
     4981 tcagcggcac aagggcgcga tcagagaccg gcgaccaggc ccgtgaccgg ggcggtgttc
     5041 agaccggtga caccctcgac ggtgccgacg gcggtgttga ccaggccgga gaccggggcg
     5101 acgccgtcca ccaggccggt gacggtgccg acggcgttca cgttcaggcc gccggagacg
     5161 ttgtcgaggt cggcgtcgga gatctcggtg gtctcaacct gggggatgga gttcatgtgg
     5221 gaacttccct tcgtatagcc atttcacaag ggggggagcg gccccctctg gggacagatg
     5281 acggccgcga ccgcggacac cgggcgcccg tttccggagc gtccccggcc tccgcggtgc
     5341 gatggatcaa agcacgcgtg ccgtccgggc ttccaatcaa tcaccccctc ccaccagggg
     5401 acttgcaacg ccgacgcgcc caacggtgca gggccgggca cgccttcggg gcgacttctt
     5461 cacaccgtgc gcgacatcgg cccggacgcc ccccttgccg tgccccggcc gattggggca
     5521 ctcccgcccc aaagacgtac cggccggtgg cgggttgtgc agatccgcca acgaggatga
     5581 ccgggctggg cattcgatgt gcagattcac tgaagcgccg attccgcccc aggatcagta
     5641 cgccgggtcg ccggccggac acggggagtg gcgagtggtg ggggtgtgcg gggagcggtc
     5701 ccgggtgcgg gggaggggcc ggtctgaacg caccgcgcgg tccgtgcgta ccaacagccg
     5761 tcaaggcgcc cccaacagca gccgggccgg tggcacggtc caccggcccc aggaggtcga
     5821 gtaggttgag tcacaagcga attccgaagc gcaaggccgc gatggccgcg ggcggtgtgg
     5881 tggcgctcgg tgcggccgcc atcctcctgc cgaacgccaa cgcctcgcag gacggcggcg
     5941 gttcacacga cgccgcggcg gccccgcgga ccctcaaggc ggcggacgcc tcggacctcg
     6001 cctcgcagct cgccggccag ctcggcgacg ccttcgccgg ctcgtactac gacgccggtg
     6061 cgcagcggct cgtcgtgaac gtcgtccccg gtgacgacga ccaggtcgcg gtgcgggccc
     6121 agcgggcggg cgcggagatc cgcgaggtcg acaacagcat gaccgagctg cacggcggcg
     6181 cgcagacact gaagtccgag gcgaccatcc ccggcacctc gtgggcgatc gacccgcgca
     6241 ccaacaagat cctggtgacc gccgacagca ccgtcaccgg cgagaagtgg gaccggcttg
     6301 agtccacggt cggccgcctc ggctccggca tggcgacgct ggagaagtcc gcgggcacct
     6361 tcaagacctt cgtgtcgggc ggcgacgcca tcttcggcgg cggcgcgcgc tgctcgctcg
     6421 gcttcaacgt caccgcgggg gacggttccc cggccttcct gacggccggg cactgcggcg
     6481 tcgcggccga cgcgtggtcc gacgcccagg acgggcagcc gatcggcacc gtcgaccagg
     6541 cggtcttccc cggcgagggc gacttcgcgc tcgtcacgta cgacgacccg gcgaccgagg
     6601 cccccagcga ggtcaacctc ggcgaccaaa ccctgcagat ctccggggcc gcggaggcga
     6661 ccgtcggcga gcaggtcttc cgcatgggca gtacgaccgg gctggccgac ggccaggtcc
     6721 tcggcctgga cgccaccgtc aactacccgg agggcacggt cagcggcctc atacagaccg
     6781 acgtctgcgc cgagcccggc gacagcggcg gctccctgtt cacgcaggac ggcctggcga
     6841 tcgggctgac gtccggcggc agcggcgact gcacggccgg cggcgagacg ttcttccagc
     6901 cggtcaccac cgccctggag gcggtcggcg ccaccctcgg cgacggcgcg ggcgacggcg
     6961 ccggggagca gaccggcggc gaggaggccg gaggggccgg cgccggcggt gacgagcccg
     7021 gcggagccgg gggcggcgat gccggtgccg gcgacgagca gcccggcggc gacgacgcgg
     7081 gcgacagcac cggcacggtc ggcgccgtct ccggttccgg ttccggcacc gacgacggaa
     7141 cgggtaccgg cgccgggcac ggctccggcc cgctcggctg accggggcgc caacgggatc
     7201 gaccggcact tcgacggcgg tccggtcctc cggcgggagg accggaccgc cgcggtgtcc
     7261 ttcgggggca ctgggccgtc gcggtgtcct gcgggagcac tgggccaccg cggtgcacgc
     7321 ccccgtccct ccccacgcgc accgtcggcg tcgtgagagg tgatcgcacc cggggccgcc
     7381 cagcgcgggg gcctcccacg catcctccgg atccagcccg cccgggtgcc gtcccggccg
     7441 ggcgcgggcg cgctcacacg cacgtacaca cgccccgcac caccggtaac agctccgcag
     7501 gggctgcgcg ggcgtcgctt tggcgctgta atggcgaagt ggtcggagag ggtgctgcgg
     7561 aacgcgggtc gaggaccctg cgggccgagg gtgccctgaa ggcgatcgcc gccggaccgg
     7621 accgccagga gcgcacgcgc cgggtcctgg agcaggcact ggtcttcgcg ggtgcctcct
     7681 gcgccgccct ctacacgccc ggcgaccacc gcgacctgct ctgcctggtc gagtcggccg
     7741 gtgtgccgcg cgccctgtac ggcgtgcgtg acagctatcc ggcgtccggc ggctccccgg
     7801 tcgccgacgc ccaccggggc ggcgccgcgg tgtgggtcgg tcctgaggag ttcgccggtt
     7861 cggcggagtc gcggcgcgtg ccgtccagcg gcctctcgct cgccgcgctg ccggtacgcg
     7921 gggacggcgg cggctgcctg ctcgccctga ccgggcatcc ggacggcttc ggcgccgagg
     7981 accgcgcctg cctggaactg atcgccgagg ccctgacctg ccccgttccc accgaggcgg
     8041 ccgagggcgg ggaactgcgg cccactgcct tcaccctcgc gatggacagc ggacgggtcg
     8101 aggtcggcga cggcgtcctg cggttgttcg gcctcgcccc cggcgagttc gacggccggg
     8161 tcgagacgct gctcggcctc acggtgccgg aggatctgcc ctcactgatg tcggtgaccg
     8221 aggccgacca catgaccatc ggcgagcgcg aactggagtt ccgcgtactg cagcccaccg
     8281 ggccgccgaa gtggctccgg ctcagcgggc gcctgctgcc cggcggcgag ggccggccga
     8341 cccgcctgct cggaaccgtc gccgacgcct ccacgctgcg cccggacgtc accgacgtcg
     8401 cccgcgtcca gcggctcgcc gccgccctcg cgaccgccgg gaccgtccgc gacgtcagcc
     8461 aggccgtggt cgccgccctg cgcaggccgc tgcgcgccga ccggatcgcg ctggccgagc
     8521 tggagaacga acggctcgtg gtcaccgtcc tcgacccgcc cgaccccgag tcctggcccg
     8581 agctgtggcg gctggagtgg cgcaccgaat ggccggacgc cccggtacgg gccatgccca
     8641 ccctcgccgc cgccctgcgc gagggccgcg cccggatctg gccggccggc accggcgacc
     8701 tggagcccgc gctcgccgac gtgggccccg gtgggctcgc cgtgctgccg ctgcccgccg
     8761 gcggacgcat ggccggcgcc tgcctcatcg gctgggacgc cccgcacgac ttcggccccg
     8821 acgaacgcgc cctgctcacc gcctccgccg gcctcgccgg acaggccctg atgcgggccc
     8881 acgccttcga cgccgaacac gaactggtcg gcatgctcca gcgccagctg ctgccgcgcc
     8941 gcctgccgga gctgcccggc gcggtcgccg tcgcccgcta cctgcccagc acggccggac
     9001 tcgaactggg cggcgactgg tacgacgtga tcccgctgcc cgaccaccac gtggccctcg
     9061 tcatcggcga cgtccagggc cacagcgccg gcgccgccac actgatgggc cagatgcgca
     9121 ccgccctgcg cgcctacgcg gccgaggggc acccgccgga cgtggtggtc tcgcacgcca
     9181 accggctgct ggtcgacatg gagaccgacc tcttcgccac ctgcgtctac gtcgacctcg
     9241 acatggaggc gggggccgcc tggtgcgtgc gggccgggca cctgccgccg gtgctgcgcc
     9301 atcccgacgg cagcacggag atcgccgagg cggagggggg cccgccgctc ggcgtggtgc
     9361 cgcaggccga cttcccgatg agtccgctgc ggctgcggcc cggcaccctg atcgccctca
     9421 ccacggacgg cctggtggag acgccggaga gcgacatcga cgcgggcctc gaccggttcg
     9481 cccacgcgct ggccggctcc gaccccgccc acctggggct cctcgccgac gccctgctcg
     9541 gcgaggcccg ccgcagcgac gacgtcgccc tgctcctggt gcgctacgac ggcatggccc
     9601 tgcgcccgct gcgggagagc tggtcggtgt ggcggctgcc ggaggccgtg cggcacgccc
     9661 gccgcttcgc ccggcgcacc ctgcgctcct gggacgtccc gggcgacacc accgacggcg
     9721 tcctcctgat cgtctccgaa ctcgtcacca acgccctcgt gcacaccgac ggccaggtcc
     9781 gcctcgacct cgccctgatc ggccgccggc tgcgcgtggc ggtcgccgac gcctcgcccc
     9841 gtacacccgc caaaccgacc agcatcggct gggaggccac cggcgggcgc ggcatcctcc
     9901 tcgtcgaggc gatgtccgcg gcgtggggaa cgctgccggt cggcgggggc aagcaggtct
     9961 ggagcgagat cgccctggag gagtgaacgg cgccccggtc cgccccggtc cgccccggtc
    10021 cggcccgtgg gccgccgtgc ccggtgtttc acggagcccg gtccggggac ccgcccgggc
    10081 gagtgcaccg gacgcggaag cgacgcgcca gaagcagaag gaagatgatg ctcgtgacca
    10141 cggacaccag cggcaaagcc gctgtccgcc gggtggagac cggatggtcg aaggcccgcc
    10201 ggctgcgggg gagggg
//
