LOCUS       NZ_CP036534            11335 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. VN1 chromosome, complete genome.
ACCESSION   NZ_CP036534
VERSION     NZ_CP036534.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. VN1
  ORGANISM  Streptomyces sp. VN1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8341703)
  AUTHORS   Nguyen,H.T., Pokhrel,A.R., Nguyen,C.T., Pham,V.T.T., Dhakal,D.,
            Lim,H.N., Jung,H.J., Kim,T.S., Yamaguchi,T. and Sohng,J.K.
  TITLE     Streptomyces sp. VN1, a producer of diverse metabolites including
            non-natural furan-type anticancer compound
  JOURNAL   Sci Rep 10 (1), 1756 (2020)
   PUBMED   32019976
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8341703)
  AUTHORS   Pokhrel,A.R., Nguyen,H.T., Dhakal,D., Nguyen,C.T., Pham,V.T.T. and
            Sohng,J.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2019) Department of BT-Convergent Pharmaceutical
            Engineering, Institute of Biomolecule Reconstruction, Sunmoon-Ro221,
            Asan, Chungnam 333-708, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2018
            Assembly Method                   :: HGAP v. 3
            Assembly Name                     :: HGAP3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011650445.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 03:53:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,284
            CDSs (total)                      :: 7,197
            Genes (coding)                    :: 7,083
            CDSs (with protein)               :: 7,083
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 44 of 114
            Pseudo Genes (incomplete)         :: 82 of 114
            Pseudo Genes (internal stop)      :: 3 of 114
            Pseudo Genes (multiple problems)  :: 15 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:32:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6350671
            Orig. end                         :: 6362006
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036534.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jae Kyung Sohng.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11335
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11335
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11335
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6335](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6335)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            92..1582
                     /gene="hemG"
                     /locus_tag="EZV63_RS28075"
                     /old_locus_tag="EZV63_28480"
     CDS             92..1582
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445289.1"
                     /locus_tag="EZV63_RS28075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28480"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_166625132.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 5e-07, bitscore:
                     28.0, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 2.6e-09, bitscore: 35.8,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCASENPSEDPAGERPADPEHVVVVGAGIAGLAAAHRLLEHGVRV
                     TVVEASDRVGGKLLPGEIAGVRVDLGAESMLARRPEAVGLARAAGLADRLQPPSTATAS
                     LWTRGALRPMPKGHVMGVPGTAAALSGVLSEEGLARIGRDAELPRTEVGEDVAVGEYVA
                     ARLGREVVDRLVEPLLGGVYAGDAYRISLRSAVPQLFEAARTHTSLTEAVRALQGRTAT
                     SPPSGPVFTGIEGGVGTLPLAVAESVRARGGEIVTGAPVTELRHTAGGGWRVVAGDRVR
                     HAAAVVVAVPARPAAELLRAEAPAAAAELSAVEYASMALITLAYRRSDAAALPEGSGFL
                     VPPVDGHTIKASTFASRKWGWIADEDPDLVVLRTSVGRYGDTEILGRDDAGLVAVSRHD
                     LREATGLAATPVATRVTRWQDGLPQYPVGHHARVARVREHVAGLPGLAVCGAAYDGVGI
                     PASIASAYAAVDQVRGDLRGVEELTAHPVQSLHGGAGE"
     gene            1587..2318
                     /gene="hemQ"
                     /locus_tag="EZV63_RS28080"
                     /old_locus_tag="EZV63_28485"
     CDS             1587..2318
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="EZV63_RS28080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28485"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_031042924.1"
                     /transl_table=11
                     /translation="MSDDASTPAAERTPNKGKLAKDLNEVIRYTLWSVFKLKDTLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDLSGLRADADLMIWWHAETADQLQEAYNLFRRTR
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYISVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRGSEARRHVR
                     EEIPFYTGRRKEIGELVAGLA"
     gene            complement(2368..3951)
                     /locus_tag="EZV63_RS28085"
                     /old_locus_tag="EZV63_28490"
     CDS             complement(2368..3951)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (110-485). E-value:
                     9.1e-20. Score: 63.2. Matches aSDomain:
                     nrpspksdomains_EZV63_RS28085_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034041.1"
                     /locus_tag="EZV63_RS28085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28490"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_166625133.1"
                     /sec_met_domain="PF00561 (E-value: 1e-22, bitscore: 79.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYSAAGSLLLTTLTAGPARAATPAGPESAEARGTAVAAGR
                     AAAAGITFGPCPKAEELPGGIRCGTVSVPLDYAHPDGKQIELAVSRVPATHKDPDNGKH
                     KVPGQGALLYNPGGPGASGLYFPLVGMVPEWKRIAAAYDLVGYAPRGVAPSAPLSCQDP
                     GDFFKGPRPAPTQPSDAYKAERAAEAETYAHDCAARAGDELRHYHSLNNARDLEVVRAA
                     LGERRLTFMGASYGTYLGAVYAELFPSHVRRMVFDAVVNPEPEQVWYRNNLDQSEAFED
                     RWDDFKEWVAEHDAEYGLGDTPRKVQDGYDEASARLAAEPAGGTVGPAQLQGAMLKAGY
                     YDDYWPHRAEALSAYLKGDPQPLVDQAGTHTEEGSVAAENSNAVYTAVECNDAPWPTDF
                     RVWDRDNTRLARRAPFETWDNVWANLPCAYWRAPRQQPLDVRTAPGELPPTLLLAAERD
                     AATPYEGALELRRRLSGSVLVTERDAGTHGIAAGPNACVNDHLEAYLLEGRLPARDTSC
                     TPRPEPRPRT"
     aSDomain        complement(2497..3621)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EZV63_RS28085_Abhydrolase_1.1"
                     /evalue="9.10E-20"
                     /label="EZV63_RS28085_Abhydrolase_1.1"
                     /locus_tag="EZV63_RS28085"
                     /protein_end="485"
                     /protein_start="110"
                     /score="63.2"
                     /tool="antismash"
                     /translation="ALLYNPGGPGASGLYFPLVGMVPEWKRIAAAYDLVGYAPRGVAPS
                     APLSCQDPGDFFKGPRPAPTQPSDAYKAERAAEAETYAHDCAARAGDELRHYHSLNNAR
                     DLEVVRAALGERRLTFMGASYGTYLGAVYAELFPSHVRRMVFDAVVNPEPEQVWYRNNL
                     DQSEAFEDRWDDFKEWVAEHDAEYGLGDTPRKVQDGYDEASARLAAEPAGGTVGPAQLQ
                     GAMLKAGYYDDYWPHRAEALSAYLKGDPQPLVDQAGTHTEEGSVAAENSNAVYTAVECN
                     DAPWPTDFRVWDRDNTRLARRAPFETWDNVWANLPCAYWRAPRQQPLDVRTAPGELPPT
                     LLLAAERDAATPYEGALELRRRLSGSVLVTERDAG"
     gene            complement(4077..4883)
                     /locus_tag="EZV63_RS28090"
                     /old_locus_tag="EZV63_28495"
     CDS             complement(4077..4883)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133765.1"
                     /locus_tag="EZV63_RS28090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28495"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_166625134.1"
                     /transl_table=11
                     /translation="MFWVLFLLSAWAVAGLACLRLCLAAVRAAAAAAAAPRSAAREHAL
                     TLYEAAFLSGGPRRVADLTLVSMARQRRLLLAHTGWATVVDPCGRDDMERSVIGAIGPE
                     GQSRIAPVRAAAATADAVRGLADRLVGAGLAVPDGGAGGVADGVRRVRAAAATVCVLGL
                     AALALPLPTTEPRLLVALWFALPLALTLGCLVITRMEVHPYSRWASPAGQRLIGALVQR
                     ADGTADDRTFLTSVAVRGVHAIGEPDLRAAFAHQEEYAEGRRERRV"
     misc_feature    complement(4885..4901)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.13"
                     /tool="antismash"
     gene            complement(5001..6335)
                     /locus_tag="EZV63_RS28095"
                     /old_locus_tag="EZV63_28500"
     CDS             complement(5001..6335)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327585.1"
                     /locus_tag="EZV63_RS28095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28500"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_194963143.1"
                     /sec_met_domain="DUF692 (E-value: 6.2e-96, bitscore: 319.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMRLGTGIGWRPEIADVVERMPGIDWVEAVSENLCPGHLPDSLLR
                     LRRRGVTVVPHGVSLGLGGAERPDAGRLAALAERARVLGAPLVTEHIAFVRAGGAPAAS
                     PLLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALFSWPDEELTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPAEALGELPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDILTDLASRVAPPGVLLERDENFPEGEELAGEVASIRAALAEGRAGGASG
                     GPDPAGPRRAVPRGSGAVAGAGDAVRQRLGRAQHALLASLVAGAPVPEGFDRARLGVQA
                     RALAGKRADVVAKVAPELPVILGPRYRPAFLAYARARPMTGGYRRDALDFAEHVLRAGA
                     GPEGTFRDVRRELREWWRERSGPVPRSARPGARLARATRRVLLRR"
     gene            complement(6461..7195)
                     /locus_tag="EZV63_RS28100"
                     /old_locus_tag="EZV63_28505"
     CDS             complement(6461..7195)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030616.1"
                     /locus_tag="EZV63_RS28100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28505"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_194963068.1"
                     /transl_table=11
                     /translation="MPVPVTDPASVPAPDSPFRAEAGDRDLAPQFVLPLVVRIERAAPP
                     HRTDALETAARAVLVMLGDERSTGDGEWARAVRDWQDARIRKVVRRARGAEWRRAEALP
                     GITVGGKTAEVRVFPPVPLDGWPKDLARLQVSGTDLDDPEPPAGADPATPVLWMNPDLD
                     MSAGKAMAQAGHGAQLAWWELSDGERAAWRDAGFPLSVRTADPSRWSGLTTGGLPVVRD
                     AGFTEIAPGSCTVVADHPALRR"
     gene            7263..8117
                     /locus_tag="EZV63_RS28105"
                     /old_locus_tag="EZV63_28510"
     CDS             7263..8117
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972874.1"
                     /locus_tag="EZV63_RS28105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28510"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_037762887.1"
                     /transl_table=11
                     /translation="MRPELRLNGVGRRYGVRGPWVLRGVELDVAPGTLVRVEGTNGTGK
                     STLLRLLAGIDAPTEGRVTGRPRTAYVPERFPPALPFTALQYLTHLGSVHGLSRRSAAR
                     AAGEWLERLGAGEYAGTQLARLSKGSSQKVAVAQALLAEPELLVLDEAWTGLDAEARGE
                     LERAVAERTAAGGAVVFVDHDPRRLADAPDAVYAVRDGALHRRTAPAGVPDGPRVVVRV
                     RGPSDAVLPADVRRTVVRAEETGPGRHTLAVPASHSDVLLRTLLTARPPWHVVSVHAPE
                     DRR"
     gene            8114..8788
                     /locus_tag="EZV63_RS28110"
                     /old_locus_tag="EZV63_28515"
     CDS             8114..8788
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972873.1"
                     /locus_tag="EZV63_RS28110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28515"
                     /product="ABC transporter"
                     /protein_id="WP_166625136.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLLRSQRWLPPVVLYAAALGIGVQGGQPVLDSLGW
                     SAAVLLPVAAWLVRISVTGEPSAARGCAAAARGPGRAHLASVLVAPAAAVVLGAAATVV
                     VALISDPVSTGRGTRVALLPAAGAGLLAALVCALLGTAVGALTTWPVLRSPGRAVPALL
                     LAALLALVASGSPARAAVSGLVTGSRSGAVPLPLLPLAAAAALASAATAAACALTSRRP
                     SS"
     gene            complement(8798..8995)
                     /locus_tag="EZV63_RS28115"
                     /old_locus_tag="EZV63_28520"
     CDS             complement(8798..8995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125244.1"
                     /locus_tag="EZV63_RS28115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28520"
                     /product="hypothetical protein"
                     /protein_id="WP_037762882.1"
                     /transl_table=11
                     /translation="MSGDSDRAPDDYAPFDAARPPEAEGPPYADCVLCREPTEYPESYK
                     GITLCPVCAWQEAQRTACSG"
     gene            complement(9459..10463)
                     /locus_tag="EZV63_RS28120"
                     /old_locus_tag="EZV63_28530"
     CDS             complement(9459..10463)
                     /NRPS_PKS="Domain: PKS_ER (19-324). E-value: 6.2e-50.
                     Score: 162.0. Matches aSDomain:
                     nrpspksdomains_EZV63_RS28120_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006137928.1"
                     /locus_tag="EZV63_RS28120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28530"
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="WP_070389397.1"
                     /sec_met_domain="ADH_N (E-value: 2.2e-07, bitscore: 29.4,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.1e-16, bitscore:
                     59.7, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRVVVFDEFGGPEVMHVVEEPVVEPAAGEVRVRIEAFALNPLDR
                     MMRAGTSPATVPLPHARLGIEGTGVVDALGPGATGLEVGDPVVLAAVPDPSARGSYAEY
                     TTVPASQVVARPAGLAVTEAAAVWVGYSTAYGALVEKAGMRPGDHVLVTAASGGVGRAA
                     LQVARQIGAVPLAVTRHTANRDDLLAAGAAAVVATDRTDVVEAVRHHTGGAGADIVLDL
                     VRGPGQQDLLRAARPGGTLVAAGFLDPRPTPSPTGTPLRVFSYRSFEHTLDAVVVRRMA
                     AFLNAGVRLGALRPAVGEVFALDDIVEAHRHLERGLHAGKKIVVTVQERRDSR"
     aSDomain        complement(9492..10406)
                     /ASF="ER configuration inconclusive"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EZV63_RS28120_PKS_ER.1"
                     /evalue="6.20E-50"
                     /label="EZV63_RS28120_PKS_ER.1"
                     /locus_tag="EZV63_RS28120"
                     /protein_end="324"
                     /protein_start="19"
                     /score="162.0"
                     /tool="antismash"
                     /translation="EEPVVEPAAGEVRVRIEAFALNPLDRMMRAGTSPATVPLPHARLG
                     IEGTGVVDALGPGATGLEVGDPVVLAAVPDPSARGSYAEYTTVPASQVVARPAGLAVTE
                     AAAVWVGYSTAYGALVEKAGMRPGDHVLVTAASGGVGRAALQVARQIGAVPLAVTRHTA
                     NRDDLLAAGAAAVVATDRTDVVEAVRHHTGGAGADIVLDLVRGPGQQDLLRAARPGGTL
                     VAAGFLDPRPTPSPTGTPLRVFSYRSFEHTLDAVVVRRMAAFLNAGVRLGALRPAVGEV
                     FALDDIVEAHRHLERGLHAGKKIV"
     CDS_motif       complement(9963..10025)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EZV63_RS28120_0001"
                     /evalue="8.30E-07"
                     /label="PKSI-ER_m5"
                     /locus_tag="EZV63_RS28120"
                     /protein_end="167"
                     /protein_start="146"
                     /score="21.5"
                     /tool="antismash"
                     /translation="GDHVLVTAASGGVGRAALQVA"
     misc_feature    complement(10465..10480)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     gene            10551..10934
                     /locus_tag="EZV63_RS28125"
                     /old_locus_tag="EZV63_28535"
     CDS             10551..10934
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009192934.1"
                     /locus_tag="EZV63_RS28125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_28535"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_037762878.1"
                     /transl_table=11
                     /translation="MRNVAGPAFLADCPTRLAVEIISDKWAVLVLFGLSHRPRRHGELV
                     DLIGGISRKVLTQTLRRLQQYGLVERRAEAPRRVEYRLTDLGLTLVEPIEVLTDWARDH
                     GEAVVAFQEAAEAADPARVRAAG"
ORIGIN
        1 ggggacggac cgcacgcgcg ggaccagcgg cccgccacca gggccacccg gcgccggggc
       61 gggcgcggcg cggtctgaga cgctgaggga catgtgcgca agtgagaacc cgagtgagga
      121 cccggcgggt gagcggcccg cggacccgga acacgtggtc gtggtcggag ccggcatcgc
      181 cggactggcc gccgcccacc gcctcctgga gcacggcgtc cgggtgaccg tggtggaggc
      241 gtccgaccgc gtcggcggca agctgctgcc cggcgagatc gccggagtcc gcgtcgacct
      301 cggggccgag tcgatgctgg cccgccgtcc cgaggccgtc ggcctggccc gcgccgccgg
      361 tctcgccgac cggctccagc cgccgtccac cgcgacggcc tccctgtgga cccgcggcgc
      421 cctgcgcccc atgcccaagg gccacgtcat gggggtcccc ggcaccgccg ccgccctgtc
      481 cggcgtgctc tccgaggagg ggctcgcccg catcgggcgc gacgccgaac tgccccgcac
      541 cgaggtcggc gaggacgtgg cggtggggga gtacgtcgcg gcgcgcctgg gccgcgaggt
      601 cgtcgaccgc ctcgtcgaac cgctgctcgg cggcgtctac gcgggcgacg cctaccgcat
      661 ctccctgcgc tcggccgtgc cgcagctctt cgaggccgcc cgcacccaca cctccctcac
      721 cgaggcggtc cgcgcgctcc agggccgcac ggccacctcc ccgccgagcg ggccggtgtt
      781 cacgggcatc gagggcggcg tcggcacgct cccgctcgcc gtcgccgagt cggtccgcgc
      841 gcgcggcggc gagatcgtca ccggcgcgcc cgtcacggag ctgcggcaca cggccggcgg
      901 cggctggcgg gtcgtcgccg gggaccgggt gcggcacgcc gcggcggtcg tcgtcgccgt
      961 gcccgcacgc cccgccgccg agctgctgcg cgccgaggcg cccgccgccg cggccgagct
     1021 gtccgcggtg gagtacgcct cgatggccct gatcaccctc gcctaccgcc gctcggacgc
     1081 cgccgcgctc cccgagggca gcggcttcct cgtaccgccc gtcgacgggc acaccatcaa
     1141 ggcgtccacg ttcgcctccc gcaagtgggg ctggatcgcc gacgaggacc cggacctggt
     1201 cgtcctgcgc acctcggtgg gccggtacgg cgacacggag atcctcggcc gcgacgacgc
     1261 gggcctcgtc gccgtctccc ggcacgacct gcgcgaggcc accggactgg ccgcgacgcc
     1321 ggtcgccacc cgcgtcaccc gctggcagga cggcctgccg cagtacccgg tcggccacca
     1381 cgcgcgcgtg gcccgcgtcc gcgagcacgt cgcgggactc ccgggcctcg cggtgtgcgg
     1441 cgcggcctac gacggcgtcg gcatcccggc gagcatcgcg agcgcgtacg ccgccgtcga
     1501 ccaggtccgg ggcgacctgc gcggtgtgga ggagctcacg gcccacccgg tgcagagcct
     1561 gcacggcgga gcgggagaat agggccatga gtgacgacgc ctccaccccc gcggccgagc
     1621 gcacccccaa caagggcaag ctggccaagg acctcaacga ggtcatccgc tacaccctct
     1681 ggtccgtctt caagctcaag gacaccctcc ccgaggaccg cgccggttac gccgacgagg
     1741 tccaggagct gttcgaccag ctcgccgcca aggacgtgac gatccgcggc acctacgacc
     1801 tgtccggcct gcgcgccgac gccgacctca tgatctggtg gcacgccgag accgccgacc
     1861 agctccagga ggcgtacaac ctcttccgcc gcaccaggct gggtcgcgcg ctggagccgg
     1921 tctggtcgaa catggcgctg caccgccccg ccgagttcaa ccgctcgcac atcccggcct
     1981 tcctggccga cgagacgccg cggaactaca tcagcgtcta cccgttcgtg cgctcgtacg
     2041 actggtacct gctgcccgac gaggaccgcc ggcgcatgct cgcggaccac ggcaagatgg
     2101 cccgcggctt cccggacgtg cgtgccaaca cggtcgcctc cttctcgctg ggcgactacg
     2161 agtggatcct cgccttcgag gccgacgaac tgcaccgcat cgtcgacctc atgcgccacc
     2221 tgcgcggctc cgaggcccgc cgccacgtcc gcgaggaaat ccccttctac accgggcgcc
     2281 gtaaggagat cggcgagctg gtggccgggc tcgcctgact ggcggcgccg taaggagatc
     2341 ggcgagctgg tcgccgggct cgcccggtca ggtcctcggc cgcggctccg ggcgtggggt
     2401 gcacgacgtg tcccgcgccg ggaggcggcc ctccagcagg tacgcctcca gatggtcgtt
     2461 gacgcaggcg ttcgggcccg ccgcgatgcc gtgcgtcccg gcgtcccgct cggtcaccag
     2521 gaccgagccc gacagccgcc gccgcagctc caacgcgccc tcgtacggcg tcgccgcgtc
     2581 gcgttccgcg gccaggagca gcgtcggcgg cagctcaccc ggcgcggtgc gcacgtcgag
     2641 cggctgctgc cggggcgccc gccagtaggc gcacggcagg ttcgcccaca cgttgtccca
     2701 ggtctcgaac ggcgcccggc gggccagccg cgtgttgtcc cggtcccaga cccggaagtc
     2761 ggtaggccac ggcgcgtcgt tgcactcgac ggccgtgtac acggcgttgg agttctccgc
     2821 cgcgaccgag ccctcctcgg tgtgcgtgcc cgcctggtcg accaacggct gcggatcgcc
     2881 cttcaggtac gccgacagcg cctcggcgcg gtgcggccag tagtcgtcgt agtacccggc
     2941 cttcagcatc gccccctgca actgcgcggg ccccaccgtc ccgcccgccg gctccgcggc
     3001 cagccgcgcg ctcgcctcgt cgtagccgtc ctgcaccttc ctcggcgtgt cgccgagccc
     3061 gtactcggcg tcgtgctcgg cgacccactc cttgaagtcg tcccagcggt cctcgaacgc
     3121 ctcggactgg tcgaggttgt tgcggtacca gacctgctcg ggctccgggt tcaccacggc
     3181 gtcgaacacc atccgccgca cgtgcgacgg gaacagttcg gcgtacaccg cccccaggta
     3241 ggtgccgtac gacgcgccca tgaaggtgag ccgccgctcg cccagcgccg cgcggacgac
     3301 ctccaggtca cgggcgttgt tgagcgagtg gtagtggcgc agctcgtccc cggcgcgtgc
     3361 ggcgcagtcg tgcgcgtacg tctccgcctc ggcggcgcgc tccgccttgt acgcgtccga
     3421 gggctgcgtc ggagcgggcc ggggcccctt gaagaagtcg ccggggtcct ggcaggacag
     3481 cggcgcggag ggcgcgacgc cgcgcggggc gtagccgacg aggtcgtagg cggcggcgat
     3541 gcgcttccac tcgggcacca tgcccaccag cgggaagtac agccccgagg cgccggggcc
     3601 gcccgggttg tagaggaggg cgccctggcc ggggaccttg tgcttgccgt tgtccgggtc
     3661 cttgtgggtg gccggtacgc ggctgacggc gagttcgatc tgcttgccgt cggggtgcgc
     3721 gtagtccagg ggcacgctga ccgtgccgca gcggatgccg cccggcagtt cctccgcctt
     3781 cggacagggg ccgaacgtga tcccggccgc cgccgcgcgc ccggcggcca cggcggtgcc
     3841 gcgtgcctcg gcggactcgg ggcccgccgg ggtcgccgcc cgggccgggc cggcggtgag
     3901 ggtggtcagc agcaaggacc cggcggccga gtagagggcg gcagctcgca tcgcgtatcc
     3961 cttcggtggc gcgggggtga cagaagggat gtttggcgcg tcggaggtgc ggggcaagca
     4021 ccggccgcgg gtgtcggcgc cgagtgcctc cgtgcgcccc cgcgggcccg gcggggtcag
     4081 acccggcgct cgcggcgccc ctcggcgtac tcctcctggt gtgcgaaggc cgcacgcagg
     4141 tccggttcgc cgatcgcgtg gacgccccgc acggcgaccg aggtgagaaa ggtccggtcg
     4201 tccgccgtgc cgtcggcccg ctgcacgagg gcgccgatca gccgctgccc ggcgggggag
     4261 gcccagcgcg agtacgggtg gacctccatg cgcgtgatga cgaggcaccc cagggtcagg
     4321 gcgagcggca gcgcgaacca gagggcgacc agaaggcgcg gctcggtggt ggggaggggc
     4381 agggccaggg cggcgaggcc gagcacgcac acggtggccg cggcggcccg cacccgccgc
     4441 accccgtccg cgacgccgcc ggcgccgccg tcgggcacgg cgaggcccgc gcccaccagc
     4501 cggtcggcca ggccccgtac ggcgtcggcg gtggccgcgg cggcccgtac cggcgctatc
     4561 cgcgactgcc cctccggccc tatcgctccg atgaccgacc gctccatgtc gtcgcgcccg
     4621 cacggatcca cgaccgtcgc ccagccggtg tgcgcgagca gcagccgccg ctgacgcgcc
     4681 atcgacacga gcgtcaggtc ggccacccgc cgcgggccgc cggacaggaa cgccgcctcg
     4741 tacagcgtca gcgcgtgctc acgggcggcg gagcgcgggg cggccgcggc cgctgccgcg
     4801 gcccgcacgg cggccaggca caggcgcaga cacgcgagac cggccacggc ccaggccgac
     4861 aggaggaaaa ggacccagaa catgacgtac ttgtatgtca aaggctttca cccgcgccat
     4921 gccctgttca cattccggga cgcggtggtg cgggtgggtg acgttccgtt cggcgacggt
     4981 cccccgcggc ggtgcgaggt tcaacggcgc agcaggacgc gcctggtcgc acgggccagc
     5041 cgggccccgg gccgggccga ccgcggtacc ggccccgagc gttcccgcca ccactcccgc
     5101 aactcccgcc gcacatcccg gaaggtgccc tccggtcccg ccccggcccg cagcacgtgc
     5161 tcggcgaagt cgagtgcgtc ccgccggtag ccgccggtca tcgggcgcgc ccgcgcgtag
     5221 gcgaggaagg ccggccggta ccgcgggccc aggatcaccg gcagttcggg cgcgaccttc
     5281 gcgaccacgt ccgcccgctt gccggccagc gcccgcgcct gcaccccgag ccgggcccgg
     5341 tcgaacccct cgggcacggg cgcccccgcc accagcgagg ccagcagcgc gtgctgcgcg
     5401 cgtccgagcc gctgccgtac ggcgtcgccg gcgccggcga cggcgccgga acctcggggt
     5461 acggcccggc gcggccccgc cgggtccggg ccgccggacg ccccgcccgc ccgtccctcg
     5521 gcgagggccg cgcggatcga cgccacctcg cccgccaact cctccccctc ggggaagttc
     5581 tcgtcccgct ccagcagcac gcccggcggt gcgacgcgcg aggcgaggtc ggtcaggatg
     5641 tcgagcaccg gccgaggaac ggggtgggcg tggctgtcgt gccagacgcc gtcccgttcg
     5701 aagccgcccg cgacatggac gtaggcgatg gcctccaggg gcagttcacc gagcgcctcg
     5761 gccgggtcct cgccgcgatt gacgtggttg gtgtgcaggt tcgcgacgtc gatcagcagc
     5821 cgcacgccgg tccggtcggc cagttcgtac aggaactgcc cctccgtcag ctcctcgtcc
     5881 ggccaggaga agagcgcggc gatgttctcc acggcgagcg ggacgggcag cgcgtcctgc
     5941 gcgatgcgca cgttctcgca cagcacgtcg agggcgtcac gggtgcgcgg cacgggcagc
     6001 aggtgcccgg cctccagcag gggcgacgcg gccggcgccc cgcccgcccg cacgaacgcg
     6061 atgtgctcgg tgaccagcgg cgccccgagt acccgggctc gctccgccag tgccgccagc
     6121 cgccccgcgt ccggccgttc ggcgccgccg agcccgagcg agacgccgtg cggcacgacg
     6181 gtcacgccgc gccggcgcag ccgcagcagc gagtcgggca ggtgcccggg acacaggttc
     6241 tcggacacgg cctcgaccca gtcgatgccg ggcatccgct cgacgacgtc cgcgatctcc
     6301 ggccgccatc cgatgcccgt ccccagtcgc atgatcggct ccatggccgc cacctcccct
     6361 cgtgcgcaca tgtcgggggt atcgcccacc cggcccgggc cgaaccccgg ccgggccgtc
     6421 ttcagagcaa catctgagct ttcgcgtggg cggctggtgg ctagcgccgc agcgccgggt
     6481 ggtccgccac caccgtgcac gagcccggcg cgatctccgt gaagcccgcg tcgcggacca
     6541 ccggaaggcc gccggtggtc aggccgctcc agcgggacgg gtccgcggtg cgtaccgaca
     6601 gcgggaaccc cgcgtcgcgc cacgccgcgc gctcgccgtc ggacagttcc caccaggcga
     6661 gctgcgcacc gtgtccggcc tgggccatcg ccttgcctgc cgacatgtcc aggtcggggt
     6721 tcatccagag caccggcgtg gcgggatcgg ccccggccgg cggctcgggg tcgtcgaggt
     6781 cggtgccgga gacctggagc cgggccaggt ccttcggcca gccgtccaac ggcaccggcg
     6841 gaaacacccg cacctcggcc gttttcccgc cgaccgtgat gccgggcagc gcctcggccc
     6901 gccgccactc ggcgccgcgg gcccgccgca cgaccttgcg gatccgcgcg tcctgccagt
     6961 cccgcaccgc ccgcgcccac tcgccgtcac cggtcgaccg ctcgtccccg agcatcacca
     7021 gcaccgcccg ggccgcggtc tccagcgcgt cggtgcggtg cggcggcgcc gcccgctcga
     7081 tgcgcacgac gagcggcagc acgaactgcg gcgcgagatc acggtccccg gcctcggccc
     7141 ggaaggggct gtcgggggcg gggacggagg cggggtcggt aacgggaacg ggcatcgccg
     7201 gatcattgct caccccgaca gtctgccaac cgtccgcccg cgccccgcga gaggatggcc
     7261 ccatgcgacc cgagctacgg ctgaacggcg tggggcgccg ctacggcgtc cgcggcccct
     7321 gggtgctgcg cggcgtcgag ctggacgtgg cgcccggcac cctggtccgc gtcgagggca
     7381 ccaacggcac cggcaagtcg accctgctgc gcctgctcgc cggcatcgac gctcccaccg
     7441 agggccgggt caccggccgg ccgcgcaccg cctacgtccc cgaacgcttc ccgcccgccc
     7501 tgcccttcac cgccctccag taccttaccc acctcggcag cgtccacggg ctgtcgcgcc
     7561 ggtcggcggc gcgtgcggcg ggggagtggc tggagcggct cggggctggg gagtacgccg
     7621 gcacacagct tgcccggctg tccaagggca gcagccagaa ggtggcggtg gcgcaggccc
     7681 tgctcgccga gccggagctg ctcgtcctcg acgaggcctg gaccgggctc gacgcggagg
     7741 cacgcggaga gctggaacgg gcggtcgccg agcggacggc ggcgggcggg gccgtcgtct
     7801 tcgtcgacca cgacccgcgc cgcctggccg acgcgcccga cgccgtgtac gcggtccgcg
     7861 acggcgccct gcaccgtcgt accgccccgg ccggcgtccc ggacgggccg cgtgtcgtcg
     7921 tccgggtgcg gggtccgtcc gatgccgtcc tgcccgccga cgtgcgccgg accgtcgtcc
     7981 gggccgagga gaccggcccc ggtcgccaca cgctcgccgt ccccgcctcc cactccgacg
     8041 tcctgctgcg cacgctgctg accgcccggc cgccctggca cgtggtgagc gtgcacgccc
     8101 cggaggaccg gcgatgacag ctctcctgcg ctaccaggcg gccctgctgc tgcgctccca
     8161 gcgctggctg ccgcccgtcg tcctgtacgc cgccgccctc ggcatcggcg tgcagggcgg
     8221 gcagccggtg ctcgactcgc tgggctggtc ggcggccgtg ctgctgccgg tcgcggcctg
     8281 gctggtgcgg atctccgtca cgggtgagcc gtccgccgcc cggggctgtg cggcggcggc
     8341 acgcggtccc gggcgggccc acctggcgtc cgtgctggtg gcgccggccg ccgcggtcgt
     8401 cctgggcgcg gcggcgaccg tcgtcgtggc gctgatcagc gaccccgtca gcaccggacg
     8461 cggcacgcgc gtggccctgc tcccggcggc gggcgccgga ctgctcgccg cgctggtgtg
     8521 cgccctgctc ggcaccgccg tcggcgcgct caccacctgg ccggtgctgc gctcgcccgg
     8581 ccgcgcggtc cccgccctgc tgctggcggc cctgctggcg ctggtggcgt cgggctcccc
     8641 ggcgcgggcg gcggtcagcg gcctggtcac cgggtcgaga tcgggcgcgg tccccctgcc
     8701 gctgctgccg ctggcggccg ccgcggcact cgcctcggcc gcgaccgcgg cggcctgtgc
     8761 gctcacctcg cgcagaccct cgtcctgagc gggcaggtca gcccgagcag gccgtgcgtt
     8821 gggcctcctg ccatgcgcag accgggcaga gcgtgatgcc cttgtacgac tccggatact
     8881 ccgtcggctc ccggcacagg acgcagtccg cgtacggagg gccctcggcc tccggcggcc
     8941 gggcggcgtc gaagggggcg tagtcgtccg gggcccggtc ggaatcgccg ctcatccctc
     9001 cagggtagac cggagtgtcc cggcgtccgc agcccccgca cacgacggcc gatctccggc
     9061 tcatccctgg ggatcgaagg cagattggcc gtatctcgcc tgccgcccct cgaccccgca
     9121 gaggctcaat gaggctcccg cgccacgcgc tgcgcgggcg gtcgtggttg aactgcgggc
     9181 gatcgccggg cagatcgtgt acgcgatcga tcacccggag tacgagttca ccgccgtcct
     9241 cgagcagcac aacaccacga acaacttcgg ttccgtcgtc aacgtgcccg gcggcaaaaa
     9301 gggtcatcgg catctaccac aacaccgctc cggacaccga gctgcgagcc gccgtggcgg
     9361 acctcacccg cctcctcggc gagctgcgtt tgggcgaggt gggcaggccc gtcatggagg
     9421 cgctgggccg gctgctcgcg ctgctgggct gacgggcctc atcgcgagtc cctccgttcc
     9481 tggaccgtga cgacgatctt cttcccggcg tgcagccccc tctccaggtg gcggtgtgcc
     9541 tcgacgatgt cgtctagggc gaacacctcg ccgacggcgg gccgcagcgc accgaggcgt
     9601 acgccggcgt tcaggaaggc cgccatgcgt ctcaccacga cggcgtcgag ggtgtgctcg
     9661 aagctccggt agctgaagac cctgagcggc gtgccggtcg gggagggcgt gggccggggg
     9721 tccaggaagc ccgcggcgac cagtgttccg ccggggcggg ccgccctcag gagatcctgc
     9781 tggccggggc ccctgacgag gtcgaggacg atgtcggcgc cggccccgcc ggtgtggtgg
     9841 cggacggctt cgacgacgtc cgtgcggtcg gtggcgacga ccgcggcggc gcccgcggcg
     9901 agcaggtcgt cccggttcgc ggtgtgccgg gtgacggcga ggggcacggc gccgatctga
     9961 cgggcgacct ggagggccgc gcggccgacg ccgccggacg cggcggtgac gaggacgtgg
    10021 tcgccgggcc gcatccccgc cttctcgacg agcgcgccgt aggcggtgga gtacccgacc
    10081 cagaccgccg ccgcctccgt gaccgcgagc ccggccggcc gggcgacgac ctggctcgcg
    10141 gggaccgtgg tgtactcggc gtagctgccc cgggcgctcg ggtccggaac ggcagcgaga
    10201 acgaccggat cgccgacctc cagcccggtg gctccggggc ccagcgcgtc gacgacgccg
    10261 gttccctcga tgccgaggcg ggcgtgcggc agggggacgg tcgcgggcga ggtgcccgca
    10321 cgcatcatcc ggtcgagcgg gttgagggcg aacgcctcga tcctgacccg tacctcgccg
    10381 gcggcgggtt cgacgaccgg ctcctcgacg acgtgcatga cctccggccc gccgaactcg
    10441 tcgaacacga cgactcgtgg catggtgact cctctgtgtc cgctgctggt ttcgttgctc
    10501 acggaagacg ctacggagcc gtcgggtacc tcctggtacc ttcgaatgtc atgcggaacg
    10561 tggctggacc agccttcctc gccgactgcc ccacgcgcct ggcggtcgag atcatctccg
    10621 acaagtgggc cgtgctcgtg ctcttcgggc tcagtcatcg gccgcgcagg cacggtgagc
    10681 tcgtggacct catcggcggc atctcgcgca aggtgctcac ccagacgctg cgccggctcc
    10741 agcagtacgg tctggtcgaa cgccgtgccg aagcgccacg gcgggtcgag taccgcctca
    10801 ccgacctggg cctgaccctc gtggagccca tcgaggtcct gacggactgg gcgagggacc
    10861 acggcgaggc cgtcgtcgcc ttccaggagg cggcggaagc ggccgacccg gcccgggttc
    10921 gcgcggcggg ctgatcgcgg gccggtcccg gaccgtcagg cgcggccgtt cgccgcgccc
    10981 accaggtccg agaccttgac gaagcggtag ccccggctgc ggagttcggg caccaccgcc
    11041 cgcaccacct cctcggtcac cggggccgcg ctgcgcgtgc agtgcatgac cacgaccgat
    11101 cccggccggg ctccgtcgag cacctgccgg gtcaccgcgt cggcgtccgt ggcgaaggcg
    11161 tcgccgctca ccacgtccca ctgcacggcg gtgacaccgg tggcggacag ttccttcagg
    11221 gccgccttgt cgtagcagcc gccggggaag cggaagtacg gcatcgggtt cggcaccccg
    11281 gccttctcga acgccgtgta cgcccgctcc aggtccgcgc gcatgtcgtc ctcgg
//
