LOCUS       NZ_CP036534            10627 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. VN1 chromosome, complete genome.
ACCESSION   NZ_CP036534
VERSION     NZ_CP036534.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. VN1
  ORGANISM  Streptomyces sp. VN1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8341703)
  AUTHORS   Nguyen,H.T., Pokhrel,A.R., Nguyen,C.T., Pham,V.T.T., Dhakal,D.,
            Lim,H.N., Jung,H.J., Kim,T.S., Yamaguchi,T. and Sohng,J.K.
  TITLE     Streptomyces sp. VN1, a producer of diverse metabolites including
            non-natural furan-type anticancer compound
  JOURNAL   Sci Rep 10 (1), 1756 (2020)
   PUBMED   32019976
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8341703)
  AUTHORS   Pokhrel,A.R., Nguyen,H.T., Dhakal,D., Nguyen,C.T., Pham,V.T.T. and
            Sohng,J.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2019) Department of BT-Convergent Pharmaceutical
            Engineering, Institute of Biomolecule Reconstruction, Sunmoon-Ro221,
            Asan, Chungnam 333-708, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2018
            Assembly Method                   :: HGAP v. 3
            Assembly Name                     :: HGAP3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011650445.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 03:53:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,284
            CDSs (total)                      :: 7,197
            Genes (coding)                    :: 7,083
            CDSs (with protein)               :: 7,083
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 44 of 114
            Pseudo Genes (incomplete)         :: 82 of 114
            Pseudo Genes (internal stop)      :: 3 of 114
            Pseudo Genes (multiple problems)  :: 15 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:32:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2650271
            Orig. end                         :: 2660898
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036534.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jae Kyung Sohng.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10627
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="10"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10627
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10627
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5627](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5627)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            409..1746
                     /locus_tag="EZV63_RS11570"
                     /old_locus_tag="EZV63_11695"
     CDS             409..1746
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978040.1"
                     /locus_tag="EZV63_RS11570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11695"
                     /product="FtsX-like permease family protein"
                     /protein_id="WP_037767197.1"
                     /transl_table=11
                     /translation="MFSLAMRSVRHRPGRFGATLLASFLGAAIIMAFNSLHDTAGAPGV
                     DGVSSETLGLAAGVVGGYGTLLVFFAVASTLTVNVRQRDEEIALLRSAGATPAQIRWMV
                     VGEAAAVAVVAALLAVGPAVLGGQALLTGFQDSGQVAKGVDHAFGPVAVASGFGVTVPA
                     SMGAAFLAVRRATRAAAGTARASGRWRRLGGWAALALGTAAVCATFTFDATDPALMAAP
                     AYGAILLSVGLAVFSPALLRVLLDRLERPLGTLFGASGLLAVQNMRRRATQLSGVLMPL
                     VLFTGMATGTLYMQAIENDAIDASGLTRSVEDKNLETLNLTVVGIIVVFSCIMLINSLY
                     AATSYRRREFGQQRLAGATPGQVLGMVGVEALVLTVSGVVLGSAAAVAGILPFSSVRAS
                     ALVPDQGLGTWLGIVAVAAAATLATSVATARRTLRTPAVRAVAVTA"
     gene            1908..2873
                     /locus_tag="EZV63_RS11575"
                     /old_locus_tag="EZV63_11700"
     CDS             1908..2873
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325456.1"
                     /locus_tag="EZV63_RS11575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11700"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_037767200.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 2.7e-51, bitscore:
                     172.2, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQQVAKEAADWSSVTGEVDVVSFDAHLPDEDALAA
                     ALADFDIVVTLRERVAFPGSLLTRLPRLRLIVASGMRNSVIDYAAAEAHGVTVCGTASS
                     SAPPVELTWALLLGLARGLVRESNGLREGGPWQQTVGADLHDRRLGLLGLGKIGSRVAQ
                     VGLAFGMRVSAWSQNLTRERADEVGVELAPSREELLRTADFVSVHLALGERTRGLLGPA
                     ELALLKPTAYLVNTSRAAIVDQDALLAALHEGRIAGAGVDVFDTEPLPPGHPMRTAPRL
                     LATPHLGYVSQANYATYYGQAVEAIRSYLAGSPVRRLPAP"
     gene            complement(2914..3489)
                     /locus_tag="EZV63_RS11580"
                     /old_locus_tag="EZV63_11705"
     CDS             complement(2914..3489)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976102.1"
                     /locus_tag="EZV63_RS11580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11705"
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_037767456.1"
                     /transl_table=11
                     /translation="MPSAVAALPALFAEESRVRVFAAVALGAGDAAQVARAAGLSAKET
                     ASALRRLREHGVVTSGDDGGLVVAQGRFRELARAARPAAEAAGTPGAAGGGREGMILRT
                     FVKDGRLVRLPARWTRKKVVLRHIAEQTFAPGVEYPERTVDGKLRVWCEDGEVDHVTLR
                     RHLVDFGHLRRSAGTYWRPAEPARAGTA"
     misc_feature    complement(3617..3632)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 16.87"
                     /tool="antismash"
     gene            3733..4011
                     /gene="chpF"
                     /locus_tag="EZV63_RS11585"
                     /old_locus_tag="EZV63_11710"
     CDS             3733..4011
                     /codon_start=1
                     /gene="chpF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028524.1"
                     /locus_tag="EZV63_RS11585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11710"
                     /product="chaplin ChpF"
                     /protein_id="WP_031040144.1"
                     /transl_table=11
                     /translation="MSRIAKAAGVVLGAGAVALSGTGMAMADAGAQGAAVGSPGVLSGN
                     VVQVPVHVPVNVCGNTIDVIGLLNPAFGNSCENESGHHSDKSGGYGG"
     gene            complement(4148..5017)
                     /locus_tag="EZV63_RS11590"
                     /old_locus_tag="EZV63_11715"
     CDS             complement(4148..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="EZV63_RS11590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11715"
                     /product="tyrosinase family protein"
                     /protein_id="WP_037767202.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTRTEKRRFVNALLEIKRRGEYDEFVRTHIEYYVS
                     DGEDGLRTAHMAPSFLPWHRRFVLDLEEALRRVDSSVTVPYWDWTKDRGAKSTPWTADL
                     LGGTGRRSDRRVTTGPFAHAEGNWTLRENVTDTEYLTRDLGRAADPIDLPSKSDLEWAL
                     DDATYDTSPYDSTVRKGFRNKLEGWGTGRGSVSWRNHNRVHRWVGGAMVGGASVNDPVF
                     WLHHAFVDLQWSRWQARHRGARYLPAEPPGRGSAQRGRRIVARHEKLPPWDVTPDELED
                     VGRIYRYA"
     gene            complement(5001..5627)
                     /locus_tag="EZV63_RS11595"
                     /old_locus_tag="EZV63_11720"
     CDS             complement(5001..5627)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028525.1"
                     /locus_tag="EZV63_RS11595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11720"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_078535917.1"
                     /sec_met_domain="melC (E-value: 7.9e-47, bitscore: 157.7,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVNAGAPANGAERKREHPPKGGGPSGNGSPGGGAADGGPASGGV
                     TSVGAASGTRRQMMRGLLAPALAVTLVPLVAASRPFRAEATAQGADAAPPGPRPAERAG
                     QETEGTAFDETYRGRRIRGIRSAAGRAVGAGTWHVTVDGRPLHLMRRADGSWLSMIDHY
                     RSYPTPLAAARGAVDELGPAEQVRDTPGDDAGHDHHTGGRHGVHA"
     gene            complement(5751..6266)
                     /locus_tag="EZV63_RS11600"
                     /old_locus_tag="EZV63_11725"
     CDS             complement(5751..6266)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="EZV63_RS11600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11725"
                     /product="hypothetical protein"
                     /protein_id="WP_031040138.1"
                     /transl_table=11
                     /translation="MRPTTRRTLAAVVTGVAAALGAAATPAAAAGAVPVPVPLDGAETA
                     LGMELPEVTGALPVPTAGAPEGPRYVEGRLLPERTLPQLPVQAGLPGADVRQPLPRVLG
                     DDFDHAALDAPAAELRTLAPGLSLDAPLTAPNPEALGLPSAKLPEVGVLTPVLQTGPVA
                     DLGVGPGL"
     gene            complement(6343..6633)
                     /locus_tag="EZV63_RS11605"
                     /old_locus_tag="EZV63_11730"
     CDS             complement(6343..6633)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325462.1"
                     /locus_tag="EZV63_RS11605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11730"
                     /product="hypothetical protein"
                     /protein_id="WP_031040135.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLVAAGSAVPAFAYDAADTAALTPTSLNGAVNA
                     LTAEPIDIMPIKHQSDALDTENKDSLLHTVKGATTDLNSAGGLLGGLPLGR"
     gene            complement(6805..7593)
                     /locus_tag="EZV63_RS11610"
                     /old_locus_tag="EZV63_11735"
     CDS             complement(6805..7593)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325463.1"
                     /locus_tag="EZV63_RS11610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11735"
                     /product="hypothetical protein"
                     /protein_id="WP_037767204.1"
                     /transl_table=11
                     /translation="MRLPARRIATSALCAGLLIGLSGPAVMAADGDPVRERTRAASLAP
                     LPDAEELQGQVGSLAGLGDMLAPVTDMLDAILKADGGRLSATDADKLAAAVKDALAKAD
                     AANTGTDTGDAATTPGTTTPGTSTPAQPPAAPDADTPVTLPAPVTARSDDPAAAAPGLA
                     ATAYAELQKQVDALTEATTAGDAAKVSPAVKEVVTGIVNVVAATMVGNGLPAADLAGLP
                     GVPTAPEATTPEATTPEATTPEAAAPEAAAPANPLPADPS"
     gene            complement(7868..8107)
                     /gene="chpG"
                     /locus_tag="EZV63_RS11615"
                     /old_locus_tag="EZV63_11740"
     CDS             complement(7868..8107)
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="EZV63_RS11615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11740"
                     /product="chaplin ChpG"
                     /protein_id="WP_007449696.1"
                     /transl_table=11
                     /translation="MSRIAKGLALTSVAAAAVAGTAGVAAADSGAQAAAAHSPGVLSGN
                     VVQVPVHIPVNVCGNTIDVIGLLNPAFGNECEND"
     gene            8370..9827
                     /locus_tag="EZV63_RS11620"
                     /old_locus_tag="EZV63_11745"
     CDS             8370..9827
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028527.1"
                     /locus_tag="EZV63_RS11620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EZV63_11745"
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_078535918.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 8.6e-22,
                     bitscore: 76.3, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLPPIGLRPRVLHVTQPVDGGVARVVTDLTRAQLAAGLRVSVAC
                     PEGGLADRLRALRADVRPWAATRSPGRALPVEVGRLARVIEEVRPDLVHAHSAKAGLAA
                     RLAVRGRVPTVFQPHAWSFEAAGGPTGVLALGWERWAARWAARTVCVSEAERLRGARAG
                     IRGRWAVVPNGVDTDRFHAGPGPGPGPDDAQDVRRRHGIGPHTPLVVCVGRLCRQKGQD
                     VLLTAWESVLARVPDARLVLAGDGPDRAALTARASAYGLLDVPGAPAPPGRHTAAPPGR
                     GTAASVLFTGAVADASGWYRAADLVVLPSRWEGMALAPLEAMACGRPVVVTDVDGAREA
                     LPPALAPRCLVPPEDPEALADAVAGLLLDAPLRASLARDGHAHVRSAHDVRHTATAVAR
                     LYGAVLDAARPSDRATAPGPHRALACDDTRTTARRPAPATAGTGARHPAPGDMGQADVP
                     RKHGMPLKRVEPLKRVEPFECRESTHP"
ORIGIN
        1 ggggtccgcg cccgggagat cctcacccag gtcggtctcg gcgaccgtct cgacgccctg
       61 cccgaccagc tctccggcgg ccagcgccag cgcgtcgcca tcgcccgcgc gctggtcacc
      121 gagcccagcg tggtcttcgc cgacgaaccg acgggagcac tcgacacgcg cagcgcccgg
      181 gacgtgctgc ggctgctgca ggaggcggtg cggctgcacg gccggaccgt cgtcatggtg
      241 acgcacgacc cggtggccgc ctcgtacgcc gactccgtcg tcttcctcgc ggacggacgg
      301 cccgccggac acctgaccgc gccgaccgtg gacgccgtcg ccgagcggct cgcgcacctg
      361 ggcgaccccg ctcccgcggg cgacaccgcc ggtctggagg tgtgagccgt gttctccctg
      421 gccatgcgtt ccgtcaggca ccgccccggg cgcttcggcg ccacgctgct cgcctcgttc
      481 ctgggcgccg cgatcatcat ggcgttcaac tcgctgcacg acaccgccgg agcgccgggc
      541 gtcgacggcg tcagctccga gacgctcggc ctcgccgcgg gcgtggtggg cggctacggc
      601 accctgctgg tgttcttcgc cgtcgcgtcc acgctgaccg tcaacgtccg gcagcgcgac
      661 gaggagatcg ccctgctgcg cagcgccggc gccaccccgg cgcagatccg gtggatggtg
      721 gtgggcgagg ccgccgcggt cgccgtggtg gcggcgctgc tggccgtcgg cccggccgtg
      781 ctcggcggac aggccctgct gaccgggttc caggacagcg gccaggtcgc gaagggcgtg
      841 gaccacgcgt tcgggccggt cgcggtggcg tccgggttcg gggtcaccgt accggcctcc
      901 atgggggccg ccttcctcgc ggtgcgtcgc gcgacgcggg ccgcggcggg cacggcgcgt
      961 gcctccggac ggtggcggcg gctgggcggc tgggcggcgc tcgccctcgg cacggcggcg
     1021 gtgtgcgcca cgttcacgtt cgacgcgacg gatcccgccc tgatggcggc ccccgcgtac
     1081 ggcgcgatcc tgctctccgt cggtctcgcc gtcttctccc cggcgctgct gcgcgtcctc
     1141 ctcgaccggc tggagcggcc gctgggcacg ctgttcgggg cgagcggcct gctggccgtg
     1201 cagaacatgc ggcggcgggc cacgcagttg tccggcgtac tgatgccgct ggtcctgttc
     1261 accggcatgg cgaccgggac gctgtacatg caggccatcg agaacgacgc gatcgacgcc
     1321 tcgggcctca cccgctccgt cgaggacaag aacctggaga ccctcaacct cacggtggtc
     1381 ggcatcatcg tggtcttctc ctgcatcatg ctgatcaaca gcctgtacgc cgcgacctcg
     1441 taccgccgcc gggagttcgg ccagcagcga ctggccgggg cgacgccggg tcaggtcctc
     1501 ggcatggtcg gtgtggaggc cctggtgctc acggtgtccg gcgtcgtcct cggcagtgcg
     1561 gccgccgtgg cgggcatcct gccgttcagc tcggtgcgcg cctccgcgct cgtgccggac
     1621 cagggcctgg ggacttggct gggcatcgtg gcggtcgccg ccgcggcgac gctggccacc
     1681 agcgtggcga cggcccggcg cacgctgcgc acaccggcgg tacgtgcggt ggcggtcacc
     1741 gcctgaccct gcaggcccgc cgtacgtacg tcgggcggac ggcaggtccg ccgtccctgc
     1801 gttgggtgcc gcgccccgtc gtacacgggg cgcggcgccc gctgtacgtt gggccgggcg
     1861 ccccgccggg ggtgtcactg cccgctcggc ggaagggacc ggacgacgtg cgactgcgct
     1921 gcgcggtact cgacgacttc cagcaggtgg cgaaggaggc cgcggactgg tcctcggtca
     1981 ccggcgaggt cgacgtcgtg tccttcgacg cccacctccc cgacgaggac gccctcgccg
     2041 ccgcgctcgc cgacttcgac atcgtggtca ccctgcgcga acgcgtggcc ttccccggct
     2101 cgctgctgac ccggctgccc cgcctgcgcc tgatcgtcgc ctccggcatg cgcaacagcg
     2161 tgatcgacta cgccgccgcc gaggcgcacg gcgtcaccgt gtgcggcacg gcgtcctcct
     2221 cggccccgcc cgtcgaactg acctgggccc tgctgctcgg cctggcccgc gggctcgtcc
     2281 gggagagcaa cgggctgcgc gagggcggcc cctggcagca gacggtgggc gccgacctgc
     2341 acgaccgccg gctcgggctg ctgggcctgg ggaagatcgg cagccgggtg gcgcaggtcg
     2401 gcctcgcctt cggcatgcgg gtgagcgcgt ggagccagaa cctcacccgg gagcgcgccg
     2461 acgaggtcgg cgtcgaactg gcgccctcca gggaagagct cctgcggacc gccgacttcg
     2521 tctccgtcca cctggccctc ggcgagcgca cccgcggtct gctgggcccc gcggaactgg
     2581 ccctgctcaa gccgaccgcc tacctggtca acacctcgcg tgcggccatc gtcgaccagg
     2641 acgccctgct ggccgcactg cacgagggcc gcatcgccgg cgccggcgtg gacgtcttcg
     2701 acaccgagcc gctgcccccc ggccacccga tgcgcaccgc cccgcgcctg ctcgccaccc
     2761 cgcacctcgg ctacgtctcg caggccaact acgccacgta ctacggccag gcggtcgagg
     2821 cgatccggtc ctacctggcc ggatcccccg tacgccgcct gcccgcaccg taggctcgcg
     2881 cggtcaggcg cttcggacag tcaggcaggc cggtcacgcg gttcccgcac gggcgggctc
     2941 cgcggggcgc cagtaggttc cggcgctccg gcgcaggtgg ccgaagtcca ccagatggcg
     3001 ccggagcgtc acatggtcga cctccccgtc ctcgcaccag acccgcagct ttccgtcgac
     3061 ggtgcgctcg gggtactcca cgccgggggc gaaggtctgc tcggcgatgt gccgcagcac
     3121 caccttcttg cgcgtccagc gcgccggaag ccggaccagc cgcccgtcct tgacgaaggt
     3181 ccgcaggatc atgccctccc ggccgccgcc ggccgccccg ggggtgccgg ccgcctccgc
     3241 cgcggggcgt gcggcgcgcg ccagttcccg gaaacggccc tgggcgacga cgagtccacc
     3301 gtcgtccccg gaggtcacca ctccgtgttc ccgcagccgg cgcagcgcac tcgccgtctc
     3361 cttcgcggag aggccggccg cgcgggccac ctgcgcggcg tcgcccgcgc ccagcgccac
     3421 ggccgcgaag acgcgcaccc ggctctcctc ggcgaagagg gcgggcagcg cggccacggc
     3481 ggacggcacg ccggccccgg tggccggagg gaacgcgtag gacaaatcgt tcgggtcgtt
     3541 cgacatgccg ggaggctagt ggacgccccg gcgcgcaggg gaacggtttt ccgtcgcccc
     3601 tccctctcac cctcccgttc aaaaagggtg atcctcacat tctccccgtg acccgctgcg
     3661 accgggtgcg ttgaacctgc agtgcggcgg cgcgtcctgc cgtcgcgctc cccgagtcag
     3721 aaggagtacg tgatgtctcg catcgcgaag gccgccggtg tcgtcctcgg cgccggtgcc
     3781 gtggcgctca gcggcaccgg gatggccatg gcggacgcgg gcgcgcaggg cgcggccgtc
     3841 ggttcgcccg gcgtcctgtc cggcaacgtc gtccaggtcc cggtgcacgt gccggtcaac
     3901 gtgtgcggca acaccatcga cgtgatcggc ctgctgaacc cggccttcgg caacagctgc
     3961 gaaaacgaga gcggccacca ctccgacaag agcggtggct acggcggctg agtgccccgc
     4021 aggcaacgaa gagccccggc cgccccggcc ggggcttttc ccctgcccgc cccgttcaga
     4081 ccttcaagcc tccaaaccct caagcctcca gaccttcaaa cctccagacc tccagacctc
     4141 caagccttca ggcgtaccga tagatccggc cgacgtcctc cagctcgtcc ggcgtcacgt
     4201 cccacggcgg cagcttctcg tgccgtgcga cgatccgtcg tccgcgctgc gcgctgcccc
     4261 ggcctggcgg ctcggcgggg aggtagcggg cgccgcggtg gcgggcctgc cagcgcgacc
     4321 actgcaggtc gacgaaggcg tggtgcagcc agaacaccgg gtcgttgacg gaggcgccgc
     4381 cgaccatggc gccgccgacc cagcggtgca cgcggttgtg gttgcgccag gagacactgc
     4441 cccggccggt gccccagccc tccagcttgt tgcggaaccc cttgcggacc gtcgagtcgt
     4501 acggcgaggt gtcgtacgtc gcgtcgtcca acgcccactc caggtcgctc ttggagggca
     4561 ggtcgatggg gtcggccgcg cgccccaggt cccgggtgag gtactccgtg tcggtgacgt
     4621 tctccctgag ggtccagttg ccctcggcgt gggcgaaggg gccggtggtg acgcgccggt
     4681 cggaacgccg cccggtgccg cccagcaggt cggcggtcca gggcgtcgac ttggcgccgc
     4741 ggtccttcgt ccagtcccag tacggcacgg tgaccgacga gtcgacccgg cgcagcgcct
     4801 cctccaggtc cagcacgaac cggcggtgcc acggcaggaa ggacggcgcc atgtgcgccg
     4861 tacgcaggcc gtcctcgccg tcggagacgt agtactcgat gtgggtgcgc acgaactcgt
     4921 cgtactcacc gcgccgtttg atctccagca gcgcgttgac gaaccggcgc ttctcggtgc
     4981 gggtcagcgt gctgacgtcc ttacgcgtgt acgccatgcc gccccccggt gtggtggtcg
     5041 tgcccggcgt cgtcgcccgg cgtgtcacgc acctgctcgg cggggccgag ttcgtcgacg
     5101 gcgccccggg ccgcggccag cggggtgggg tacgagcggt agtggtcgat catgctcagc
     5161 cagctgccgt cggcccggcg catcaggtgc agcgggcggc cgtccacggt gacgtgccag
     5221 gtgccggcgc cgacggcccg ccccgcggcg gaccggatgc cgcggatacg ccggccgcgg
     5281 tacgtctcgt cgaaggcggt gccctcggtc tcctgccccg cccgttcggc ggggcgtggc
     5341 ccgggaggcg cggcgtccgc cccctgggcg gtggcctcgg cccggaacgg ccgggaggcg
     5401 gcgaccaggg ggaccagcgt cacggcgagg gccggggcga gcagcccccg catcatctgc
     5461 cgccgcgtac cggacgccgc gccgacggat gtcaccccgc cggacgccgg tcccccgtcc
     5521 gccgcaccgc cacccggcga accgttgccc gacgggcccc cgccctttgg cggatgctct
     5581 ctcttccgct ccgcgccgtt cgccggcgca cccgcgttga cgaccatgac tcgctcctcg
     5641 tccggttccg gtggtttccc accgctaacc gcacggcggg cccagcggtc actgtccgca
     5701 gggccagtcg gagcacggcc gcgccacgga gcgcggagta cgcgtgggag ctacagcccc
     5761 gggccgacgc ccaggtcggc caccggcccc gtctgcagca cgggggtgag cacgccgacc
     5821 tcgggcagct tggcggacgg caggccgagg gcctcggggt tcggcgcggt gagcggggcg
     5881 tccagggaca ggcccggcgc cagggtccgc agctcggcgg cgggagcgtc gagggcggcg
     5941 tggtcgaagt cgtctccgag gacgcgcggc agcggctggc gcacgtcggc gccggggagg
     6001 ccggcctgca ccggcagttg cgggagagtc cgctcgggca gcagccgtcc ctcgacgtac
     6061 cggggcccct cgggggcgcc agcggtcggc acgggcagcg cgccggtcac ctccggcagt
     6121 tccatgccga gggcggtctc ggcgccgtcc agcggcaccg gcacggggac cgcgccggcc
     6181 gcggcggccg gggtcgcggc cgcacccagg gcggcggcca cgccggtgac gacggcggcc
     6241 agggttcgtc tggtggtcgg cctcatgtca ggcacggccc ctttcgagaa tgcggggaaa
     6301 cagtgggggg agagcggagg aggatgccga gggagaagcg gctcagcggc ccagtgggag
     6361 gccgccgagg aggccgcctg cggaattcag gtcggtggtc gcgcccttca cggtgtgcag
     6421 cagggagtcc ttgttctcgg tgtcgagcgc gtccgactgg tgcttgatcg gcatgatgtc
     6481 gatgggctcg gcggtcagtg cgttcacggc gccgttgagg ctggtggggg tgagggcggc
     6541 ggtgtcggcg gcgtcgtacg cgaaagcggg cacggcggaa ccggcggcga ccagggaacc
     6601 ggcgacgacg gcggcggcct tgagagactt catcgtgttc ctttcttcga cttttcctcc
     6661 ctaacgatct gccgaggacc cggaaactcc gccgtccgga agacatatga gatctccgga
     6721 gccgagactg cccgaaaagc ggaaaggccg ccgcgccgtc cgacagggag gcgcggcggc
     6781 ccgtgaggcc gacgtcggcg aaggtcagga ggggtcggcg ggcagcgggt tcgcgggcgc
     6841 ggcggcctcg ggggcggcgg cttccggagt cgtggcctcc ggagtggtgg cctcgggcgt
     6901 cgtggcctcc ggcgcggtcg gtacgccggg caggccggcc aggtccgcgg cgggcaggcc
     6961 gttgccgacc atggtggcgg cgacgacgtt gacgatgccg gtcaccacct ccttgaccgc
     7021 cgggctcacc ttcgcggcgt caccggcggt ggtggcctcg gtcagggcgt cgacctgctt
     7081 ctgcagctcg gcgtaggcgg tggccgccag gccgggcgcc gccgcggcgg ggtcgtcgct
     7141 ccgcgcggtg acgggcgccg gcagcgtgac gggggtgtcg gcgtccggcg cggccggggg
     7201 ctgggccggg gtgctggtgc cgggagtggt cgtaccgggg gtggtcgccg cgtcgccggt
     7261 gtccgtgccg gtgttcgcgg cgtcggcctt cgcgagggcg tccttgacgg cggcggcgag
     7321 cttgtccgcg tccgtggcgg agagccggcc gccgtcggcc ttgaggatcg cgtcgagcat
     7381 gtcggtgacc ggagcgagca tgtcgcccag accggccagg ctgccgacct ggccctgcag
     7441 ttcctcggcg tcggggaggg gggcgaggga ggccgcgcgg gtgcgttccc ggaccgggtc
     7501 gccgtcggcc gccatcaccg ccggacccga gagaccgatc agcaggccgg cgcagagagc
     7561 ggaggtggcg attcgccgtg cgggcagacg catgaggggg tcctttcggt ggcgcgtcgg
     7621 atcttttgcc caccgtggaa gcggccccgg cgctctgcaa ccgagcggtg acccggtgtg
     7681 ccgggccgca cgtgccgccg ttcgcgtctt acctggtgag acgccctgtc aaggtccgtg
     7741 gcccgtgtcc gcgcccggac cccccattcg gggcagcaca ccaccgtccg cgcggcaacg
     7801 cgaaccggcc gcccgctcgc ggaagaaccg cggtacggac ggccggtgcg ggagtcgcga
     7861 gccgacgtca gtcgttctcg cactcgttgc cgaacgccgg gttcagcagg ccgatgacgt
     7921 cgatggtgtt accgcagacg ttgaccggga tgtggaccgg aacctgcacc acgttgccgg
     7981 acaggacgcc cggggagtgg gcggcggcgg cctgcgcgcc gctgtcggcg gcggcgacac
     8041 cggcggtgcc cgccaccgcg gcggcggcga cggaggtcag ggccaggccc ttcgcgatac
     8101 gcgacatgga gaagtgctcc ttggggttgc gacatacatc cgggcggggg tgcccggcgc
     8161 agtgtcaacg cgtggcaggt acgggagttg tgccgccaaa ggggtgatgt cgccggggac
     8221 cgccattcac aaatccaaca cacttcccgg tttcaccgta ttagtacgga tagcggctag
     8281 agtcccacac cgttcgacgc acctctgtca cgcccctgtc gcactccgtt tgcgcccctg
     8341 tctcatccat gccgcgcaag gacacgcgta tgcacctgcc accgatcggc ctacggccac
     8401 gggttctgca tgtcacccag ccggtggacg gcggggtcgc ccgggtggtg acggacctga
     8461 cgcgtgctca gctcgccgcc gggctgcggg tgtccgtcgc ctgccccgag ggaggtctcg
     8521 ccgaccggct ccgcgccctg cgcgcggacg tgcggccctg ggcggcgacg cgctcgccgg
     8581 gccgggccct gccggtggag gtgggtcgcc tcgcccgcgt gatcgaggag gtgcggcccg
     8641 acctggtcca cgcccacagc gccaaggccg gtctggccgc gcggctcgcc gtacgggggc
     8701 gggtcccgac cgtgttccag ccgcacgcct ggtcgttcga ggcggccggc ggacccaccg
     8761 gggtcctcgc gctcggctgg gagcggtggg ccgcgcggtg ggcggcgcgc acggtgtgcg
     8821 tgagcgaggc ggaacggctg cgcggggcgc gggccgggat ccgcgggcgc tgggcggtgg
     8881 tcccgaacgg cgtcgacacg gaccgcttcc acgccggacc cgggcccggc cccggcccgg
     8941 acgacgcgca ggacgtacgg cgacggcacg gcatcggccc gcacaccccg ttggtggtct
     9001 gcgtggggcg gctgtgccgg cagaagggcc aggacgtgct gctcaccgcg tgggagtcgg
     9061 ttctcgcgcg cgtgccggac gcccggctgg tcctggccgg tgacggaccg gaccgcgccg
     9121 cgctcaccgc gcgggcatcg gcgtacggcc tcctcgacgt cccgggcgcc cccgcgccac
     9181 ccggccggca caccgccgcg ccacccggcc ggggcaccgc ggcctccgtg ctgttcacgg
     9241 gtgccgtggc cgacgcctcc ggctggtacc gggccgccga cctggtcgtc ctgccgtcgc
     9301 gctgggaggg catggcgctg gccccgctgg aggcgatggc ctgcgggcgc cccgtggtgg
     9361 tgaccgacgt ggacggagcc cgcgaggccc tgccgcccgc gctcgcgccg cgctgcctgg
     9421 tgccgcccga ggacccggaa gccctggccg atgcggtcgc cggcctcctg ctcgacgcgc
     9481 ccctgcgcgc gtcgctcgcc cgcgacggcc acgcccacgt ccgctccgcg cacgacgtac
     9541 ggcacacggc cacggcggtc gcacggctgt acggcgccgt gctcgacgcc gcacgtccgt
     9601 ccgaccgggc cacggccccc ggtccccacc gggccctggc ctgcgacgac acccggacga
     9661 ccgcgcggcg cccggccccc gccaccgccg ggaccggcgc gcgacacccg gcccccggcg
     9721 acatggggca ggccgacgtg ccccgcaagc acggcatgcc cctcaagcgc gtcgagcccc
     9781 tcaagcgcgt cgagcccttc gagtgcaggg agtccaccca cccgtgaccg cggaaagcac
     9841 cgtccccacc cccgccggcc ggcctcgggg cctcggcccg tcccccgtct cggtgttgcc
     9901 gcagcgggcc ggtgcggcgg gcggcggccc gcgactgccc gcccggcgta ccgccgcacg
     9961 gcccgactcc ccgctgccgt tgctcgtcgc ggacggcacg gcggccctgc cgggctcgct
    10021 gatgctcacc ggcgccccgc accagccact gctcaccgcc ctgctggtcg gcacctcgct
    10081 cctgctgcgc ccaaagccgc acccggccgc cgcccggccg gtctccggcg tgctggacga
    10141 actgccggcg ctgtgcgccc gtatcgcggt ggtctggctg gcgctggcgg cgctcctcgc
    10201 ggcgtacgcc ccggcgcacg cgctgtccgc ccgcacgctg ctcgccggtt tcctgctgca
    10261 cgcggcgacg ggctgcgccg gccggggcgc cgtgcaccgc aggcgccgca gggcgctgtc
    10321 gaaccgtccc cgcgcggccc tcgtcgtcgg gcccgccgcg accgcccagc gggtggcgtc
    10381 ggcgctgctg cgccacccgg gctgcggggt gcgtcccgtc ggggtcgtca ccgacgaccc
    10441 cgacggcacc acgggcctgc ccgtgctgtc cacgggtgag gaggcgcggc gggcggtggt
    10501 gcagaacggc gtgcgcgacg tcctcgccgt ggcccccgcc gtacggcccc ggcaggcggc
    10561 gctgctgcgg acgctggccg agtcgggctg cacggtgtgg gaggtcgacg cggacgccca
    10621 cgcgcac
//
