LOCUS       NZ_WHZR01000001        10399 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces sp. OM5714 A912_AS4_SC1_R2, whole genome shotgun
            sequence.
ACCESSION   NZ_WHZR01000001
VERSION     NZ_WHZR01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. OM5714
  ORGANISM  Streptomyces sp. OM5714
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915160)
  AUTHORS   Samborskyy,M.
  TITLE     Whole Genome Sequence of the phthoxazolin producer Streptomyces sp.
            OM5714
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915160)
  AUTHORS   Samborskyy,M., Dickens,S. and Scott,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2019) Biochemistry, DNA Sequencing Facility,
            University of Cambridge, 80 Tennis Court Road, Cambridge,
            Cambridgeshire CB2 1GA, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2017
            Assembly Method                   :: Newbler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 102.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010993835.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:48:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,820
            CDSs (total)                      :: 7,730
            Genes (coding)                    :: 7,557
            CDSs (with protein)               :: 7,557
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 7, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 7, 5 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 58 of 173
            Pseudo Genes (incomplete)         :: 139 of 173
            Pseudo Genes (internal stop)      :: 18 of 173
            Pseudo Genes (multiple problems)  :: 36 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:03:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6308120
            Orig. end                         :: 6318519
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHZR01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="23"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    678..693
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.08"
                     /tool="antismash"
     gene            1439..2047
                     /locus_tag="STPH1_RS28045"
                     /old_locus_tag="STPH1_5563"
     CDS             1439..2047
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="STPH1_RS28045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_5563"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_164385231.1"
                     /transl_table=11
                     /translation="MNLELPELPFTLRSYGPAGDWSYAGGVLTGVAGARQDRFVPPTGE
                     SLESVSDAPRLLGAPEGDFQLIARLSVGFRDAFDAGVLYVHVGERAWAKLCLEYSPDAA
                     TVCTVVTRGHSDDCNSFTVDGDSVWFRVSRSGRAFAFHASLDGERWTFVRLFTLGDEEE
                     TGAALVGFLAQAPTGEGCTVTYDHLEFRPGWPADLRDGS"
     gene            2139..2852
                     /locus_tag="STPH1_RS28050"
                     /old_locus_tag="STPH1_5564"
     CDS             2139..2852
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448152.1"
                     /locus_tag="STPH1_RS28050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_5564"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_164385232.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDGVEVVHRSFELDP
                     GRAKDDVQPVLTMLTAKYGMSEAQAQAGEDNLGAQAAAEGLDYRTRDRDHGSTFDMHRL
                     LHLAKERGRHEALLDAFYRGNFADERSVFNDDERLLELAVGAGLDAGEVRAVLDDPGAY
                     ADEVRADEREAAQLGATGVPFFVLDRAYGVSGAQPAEVFSQALTQAWGGRTPLKLIDDG
                     GAEACGPDGCAVPQE"
     gene            2924..4018
                     /locus_tag="STPH1_RS28055"
                     /old_locus_tag="STPH1_5565"
     CDS             2924..4018
                     /NRPS_PKS="Domain: Aminotran_5 (28-358). E-value: 1.8e-24.
                     Score: 78.3. Matches aSDomain:
                     nrpspksdomains_STPH1_RS28055_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448151.1"
                     /locus_tag="STPH1_RS28055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_5565"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_205616752.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.5e-18, bitscore:
                     64.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGEVTGPHSGAMESFENLVRTEFAPKTTYLNTASTGLLPARTLV
                     AMRDAAASAADGRPLDMFADVEAARAACARLVGVPATRVATGASVATFSGLVAASLPTG
                     AEVLTAEADFSSLVNPLYARGDLKVRSVPLERIAESVGPDTALVAVSSVQSADGRIADL
                     AGLRDAARAHGARTLIDASQAGGWLPTAADDFDYVTAVGYKWVLCPRGVAFLVVPEDLG
                     AAAGLTPLFAGWATGERPWDSCYGPVEEYAHSARRFDESPALFSYTGARHSLALIEELG
                     VDAVHAHDTALADRFRVGVRSLGHEPVDAPGSAIVSVPGLGRRQAELGEAGVQVSDRAG
                     NLRAAFHVYNTTADVDRLLDVLAG"
     aSDomain        3008..3997
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STPH1_RS28055_Aminotran_5.1"
                     /evalue="1.80E-24"
                     /label="STPH1_RS28055_Aminotran_5.1"
                     /locus_tag="STPH1_RS28055"
                     /protein_end="358"
                     /protein_start="28"
                     /score="78.3"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARTLVAMRDAAASAADGRPLDMFADVEAARAAC
                     ARLVGVPATRVATGASVATFSGLVAASLPTGAEVLTAEADFSSLVNPLYARGDLKVRSV
                     PLERIAESVGPDTALVAVSSVQSADGRIADLAGLRDAARAHGARTLIDASQAGGWLPTA
                     ADDFDYVTAVGYKWVLCPRGVAFLVVPEDLGAAAGLTPLFAGWATGERPWDSCYGPVEE
                     YAHSARRFDESPALFSYTGARHSLALIEELGVDAVHAHDTALADRFRVGVRSLGHEPVD
                     APGSAIVSVPGLGRRQAELGEAGVQVSDRAGNLRAAFHVYNTTADVDRL"
     gene            complement(4098..4994)
                     /gene="thpD"
                     /locus_tag="STPH1_RS28060"
                     /old_locus_tag="STPH1_5566"
     CDS             complement(4098..4994)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="STPH1_RS28060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_5566"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_164385234.1"
                     /sec_met_domain="PhyH (E-value: 2.6e-41, bitscore: 140.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTHVTDLYPTRGATEVATPRQDPVVWGSPDTPGPVSAGDLQA
                     LDRDGFLAIDQLIGPDEVAEYQRELERLTSDPAIRADERSIVEPQSKEIRSVFEVHKIS
                     EVFAKLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPN
                     MRTISVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEGL
                     TKMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFAA
                     PIRRPDFIGARDFTPVK"
     misc_feature    complement(4985..5005)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.15"
                     /tool="antismash"
     gene            complement(5001..5399)
                     /locus_tag="STPH1_RS28065"
                     /old_locus_tag="STPH1_5567"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="STPH1_RS28065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_5567"
                     /product="ectoine synthase"
                     /protein_id="WP_003976954.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.6e-54, bitscore:
                     179.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEFEGTDRHVKSASGTWESTRIVLAKEKVGFSVHETILY
                     AGTETSMWYANHIEAVVCTKGDAELTDRETGKTYHITPGTMYLLDGHERHTLKVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            complement(5498..6769)
                     /gene="ectB"
                     /locus_tag="STPH1_RS28070"
                     /old_locus_tag="STPH1_5568"
     CDS             complement(5498..6769)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-366). E-value: 6e-72.
                     Score: 234.4. Matches aSDomain:
                     nrpspksdomains_STPH1_RS28070_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="STPH1_RS28070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_5568"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_007448159.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.7e-76, bitscore:
                     256.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAVGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNAVLKRALIDYLERDGVTHGLDMSTTAKRRFLETFQNTILRKRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFIWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALAALCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGMPMA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTATATLEAYWADGSAMEKQTRKRGEQVEQHM
                     IAITEENLADVKEYRGRGLVWGLEFHDKDRAGRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGMKTLARAVRETA"
     aSDomain        complement(5672..6682)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STPH1_RS28070_Aminotran_3.1"
                     /evalue="6.00E-72"
                     /label="STPH1_RS28070_Aminotran_3.1"
                     /locus_tag="STPH1_RS28070"
                     /protein_end="366"
                     /protein_start="29"
                     /score="234.4"
                     /tool="antismash"
                     /translation="DRAVGSRMYDEDGHEYLDFFAGAGSLNYGHNNAVLKRALIDYLER
                     DGVTHGLDMSTTAKRRFLETFQNTILRKRDLPYKVMFPGPTGTNAVEAALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFIWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALAALCERQDMLLIVDDIQMGCGRT
                     GAFFSFEEAGITPDIVTVSKSISGYGMPMALTLFKPELDVWEPGEHNGTFRGNNPAFVT
                     ATATLEAYWADGSAMEKQTRKRGEQVEQHMIAITEENLADVKEYRGRGLVWGLEFH"
     misc_feature    complement(6857..6871)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 24.27"
                     /tool="antismash"
     gene            complement(6899..7411)
                     /gene="ectA"
                     /locus_tag="STPH1_RS28075"
                     /old_locus_tag="STPH1_5569"
     CDS             complement(6899..7411)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448158.1"
                     /locus_tag="STPH1_RS28075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_5569"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_153180480.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPRVADGAALWRIARDSEVLDLNSSYSYLLWCRD
                     FAATSAVVRDGHGVPVGFITGYVRPDSPDTLLVWQVAVDGTYRGRGLAATLVDGLAERV
                     ARERGITTLETTISPDNTASQRLFTSFAERRGARLEREVLFDTAVFPDGPHEPEVLYRI
                     GPLSAGG"
     misc_feature    complement(7410..7422)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    7421..7436
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     misc_feature    complement(7436..7448)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7567..7582)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7577..7592
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     gene            complement(7741..8868)
                     /locus_tag="STPH1_RS28080"
                     /old_locus_tag="STPH1_5570"
     CDS             complement(7741..8868)
                     /NRPS_PKS="Domain: Aminotran_5 (51-309). E-value: 4.6e-29.
                     Score: 93.4. Matches aSDomain:
                     nrpspksdomains_STPH1_RS28080_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976957.1"
                     /locus_tag="STPH1_RS28080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_5570"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_164385235.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.5e-21, bitscore:
                     75.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAGRFAAIEDRVARLLGTGQDVVVMQGEALLPLE
                     GAIRAAAGPGTVALNVVTGPYGQTFGNWLRDCGATVHDLAVPFHTAVSAERIGEALAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRRALLHAP
                     AQLEMLALEACVERIEAEGAGTVMARHASAAAATRAGALALGGLEPYVYEAKDAAPVAT
                     TLRTPSDVDASALVARALESDPGLPLAAGGGALAGEMIRVNHYGPDATREAVRGCLAAL
                     GAALAERGLKADPEAARRAVEEAWERPAGTGPSEG"
     aSDomain        complement(7942..8715)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STPH1_RS28080_Aminotran_5.1"
                     /evalue="4.60E-29"
                     /label="STPH1_RS28080_Aminotran_5.1"
                     /locus_tag="STPH1_RS28080"
                     /protein_end="309"
                     /protein_start="51"
                     /score="93.4"
                     /tool="antismash"
                     /translation="AGPGTVALNVVTGPYGQTFGNWLRDCGATVHDLAVPFHTAVSAER
                     IGEALAEHPEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPD
                     AWGVDLCVIGAQKAMGGPAGVSAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGG
                     RRALLHAPAQLEMLALEACVERIEAEGAGTVMARHASAAAATRAGALALGGLEPYVYEA
                     KDAAPVATTLRTPSDVDASALVARALESDPGLPLAA"
     gene            9109..9639
                     /locus_tag="STPH1_RS28085"
                     /old_locus_tag="STPH1_5571"
     CDS             9109..9639
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138951.1"
                     /locus_tag="STPH1_RS28085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_5571"
                     /product="hypothetical protein"
                     /protein_id="WP_164385236.1"
                     /transl_table=11
                     /translation="MLALVLLVLVAAATAAVLRRGAGTYPRHPAAVAAARVPRLPGAPL
                     RGVAGAAGGAAALLYLWGLLCVGGAVMEAEDGGTDSVPPRPCRDGVPPERSGQVVDYSV
                     SYLPLGFRCETLDGDGYDSADIPGHLNPGVAVLALTAVGSAVAAGYATERRARAAARSD
                     GRAPASNRRTGSL"
ORIGIN
        1 cgccgacgcg gagaccgcgc tcggcccggt ccggctcacc ggcgaactcg cccgcacctg
       61 ctaccagctg cgggccttcg ccgacatcgt cgacgagggc gagttcctcg gggtggtcgt
      121 caaccatccc gacgacaccg ccaccccgcc catccccgac ctgcgccgct acaaggtgcc
      181 gctcggcgtc gtcgccgtct actcggcctc caacttcccc ttcgccttct ccgtccccgg
      241 cggcgacacc gcgagcgcgc tggcggccgg ctgcccggtc gtcgtcaagg cccaccccga
      301 ccacccggcc ctgtccgagc tggtcgccaa ggtgctgcgc cgggccgcgg cccgccacga
      361 catcccggac ggtgtcctcg gtctggtgca cggcttcgac gccggcgtcg agctgatcaa
      421 gcacccgctg gtcgccgcgg cgggcttcac ggggtcggtg cgcgggggcc gcgcgctgtt
      481 cgacgcggcg gcggcgcgtc cggtgccgat ccccttccac ggcgagctgg gctcactgaa
      541 cccggtcgtc gtcacggagg cggcggccgc ggagcgggcg gaggcgatcg gcacggggct
      601 ggccggttcc atgacgctgg gcgtcgggca gttctgcgtg aagcccggcc tggtgctcgt
      661 gccggccggt gacgccggtg acggcctggt caagtccctc accgacgcgg tcagcgccac
      721 cgacgccggt gtgctgctcg accaccgtat gcgggacaac ttcgtcgcgg gcgtcgccga
      781 gcgcgccgcg ctgcccgacg tggactcccc ggtgacgccg ggcgcgggcg gcgagcacac
      841 cgtcagcgcc gggttcctca cggtgccggc ggcgcgactg gcggcggagg gcgcgcacga
      901 cctgctcctg gaggagtgct tcgggccggt caccgtggtg gcccgctatc aggacgagga
      961 ggaggtgcgg ggggtgttgt cgcggctgcc ggggaacctg acggcgaccg tgcagctctc
     1021 tgccgaggaa gtggcgggag agggcgccgg ggcggctctc ctcgcggagg tgacgccgct
     1081 ggcggggcgg gtgctggtca acggctggcc cacgggggtc gccgtggcgg cggctcagca
     1141 gcatgggggg ccctacccgg cgaccacgtc gacgtccacg tcggtggggg gtacggccat
     1201 cgagcggtgg ttgcggccgg tggcctatca ggggacgccc gaggcgctcc ttccggtgga
     1261 gcttcgggac gacaatccgc tggggctgcc gcggcggttc aacggggtgc tggagcggta
     1321 gcgctccgcg ggtgcgccgt aggggtgcgg ttgttcggcg ggtgctgcgc cgtcgtggcc
     1381 ggtcgcgcag ttccccgcgc cccttgggac gacgttcccg gcctgcgatg atcaggccat
     1441 gaatctcgaa ctccccgaac tgcccttcac cctccgttcc tacgggcccg ccggtgactg
     1501 gtcctacgcc ggcggtgtgc tcaccggcgt cgccggggcc cggcaggacc ggttcgtgcc
     1561 gccgaccggg gagtcgctgg agtccgtgtc cgacgcgccg cggttgctgg gggcgcccga
     1621 gggggacttc cagctgatcg cgcggctctc cgtcgggttc cgggacgcct tcgacgccgg
     1681 ggtgctctac gtgcacgtgg gggagcgggc ctgggccaag ctgtgtctgg agtactcccc
     1741 ggacgcggcc accgtctgca cggtggtcac ccgggggcac tccgacgact gcaactcctt
     1801 caccgtcgac ggagactccg tctggttccg ggtcagccgc agcggacggg ccttcgcctt
     1861 ccacgcctcc ctcgacggtg agcgctggac cttcgtccgg ctcttcacgc tcggcgacga
     1921 ggaggagacc ggcgccgccc tggtcggctt cctggcacag gcgccgacgg gggaggggtg
     1981 cacggtgacg tacgaccacc tggagttccg gccgggttgg cccgcggatc tgcgggacgg
     2041 cagctgagcg cggggcggga atgagcgcgg ctggttgaag gttcacgtac gaggcggaga
     2101 gggtctccgc ctcgtacgag ctggagaaga gccgagacat gcgcgtcgag atctggagcg
     2161 acatcgcctg cccctggtgc tacgtgggca aggcccgctt cgagaaggcg ctcgcggcct
     2221 tcccgcaccg ggacggggtc gaggtggtcc accgttcctt cgagctggac cctgggcggg
     2281 ccaaggacga cgtgcagccg gtgctgacga tgctcaccgc gaagtacggc atgagcgagg
     2341 cgcaggcgca ggccggtgag gacaacctcg gtgcgcaggc cgccgcggag ggcctggact
     2401 accgcacccg ggaccgcgac cacggcagca ccttcgacat gcaccggctg ctgcacctcg
     2461 ccaaggagcg gggccggcac gaggcgctgc tcgacgcctt ctaccggggc aacttcgcgg
     2521 acgagcgctc cgtgttcaac gacgacgagc ggctgctgga gctggccgtc ggcgccgggc
     2581 tcgacgccgg ggaggttcgg gcggtgctcg acgatcccgg cgcctacgcc gacgaggtgc
     2641 gcgccgacga gcgcgaggcc gcccagctcg gggccaccgg ggtgccgttc ttcgtcctcg
     2701 accgcgccta cggcgtctcg ggcgcccagc ccgccgaggt cttcagccag gcgctgaccc
     2761 aggcgtgggg cgggcgcacg ccgctgaagc tgatcgacga cgggggagcg gaggcgtgcg
     2821 gtccggacgg gtgcgccgtg ccccaggaat gaagggcggg gcgaaggccc aggtgagagg
     2881 ccgttcataa gcgttgcttg gaggaaccgc gtagaaatac gcgatggacg gggaggtcac
     2941 ggggccgcac agtggtgcca tggagagctt cgagaatctc gtccgcaccg agttcgcccc
     3001 gaagaccacg tatctgaaca ccgccagtac cggcctgctc cccgcccgga ccctcgtcgc
     3061 gatgcgggac gcggcggcct cggcggcgga cgggcggccg ctcgacatgt tcgccgacgt
     3121 cgaggcggcc cgcgccgcct gtgcccgcct cgtgggggtg cccgcgacgc gggtcgcgac
     3181 cggcgcgtcc gtcgccacgt tcagcgggct cgtcgccgcg tcgctgccga cgggcgccga
     3241 agtgctcacc gcggaggccg acttcagctc cctggtgaac ccgctgtacg cgcgcggcga
     3301 cctcaaggtg cgcagtgtgc cgctggagcg gatcgccgag tccgtggggc cggacaccgc
     3361 gctcgtggcg gtcagttccg tgcagtccgc cgacggacgg atcgccgacc tcgccgggct
     3421 gcgggacgcc gcccgggcgc acggggcgcg gacgctgatc gacgcctccc aggcgggcgg
     3481 ctggctgccg acggccgcgg acgacttcga ctacgtgacc gccgtcggct acaagtgggt
     3541 gctctgcccg cgcggcgtcg ccttcctcgt cgtaccggag gacctgggcg ccgcggccgg
     3601 gctgacgccg ctgttcgccg ggtgggcgac cggtgagcgc ccctgggaca gctgctacgg
     3661 gcccgtcgag gagtacgcgc actccgcccg gcggttcgac gagtcccccg ccctgttctc
     3721 gtacaccggc gcccggcact ccctggccct gatcgaggaa ctgggggtcg acgccgtgca
     3781 cgcccacgac accgcgctcg ccgaccggtt ccgcgtgggg gtgcgctcgc tggggcacga
     3841 gccggtggac gcgccgggtt cggcgatcgt gtccgtgccg gggctcggcc gtcggcaggc
     3901 ggaactgggc gaggccggcg tccaggtgtc cgaccgggca ggcaatctgc gggccgcctt
     3961 ccacgtgtac aacaccacgg ccgacgtgga ccggctgctc gacgtactgg cgggctgaga
     4021 gaagaaggcc ttgaacggca tcgggcaggg gcctcccgtc ccacacgaaa aggcccctgc
     4081 ccgagatcag atgcgggtca cttcaccggg gtgaagtccc gcgcgccgat gaagtccggc
     4141 cgccggatcg gtgccgcgaa cggctccacc gccgtgttct ccacgctgtt gaacacgatg
     4201 aacacgttgc tgcgcgggaa cggcgtgatg ttgtcgcccg acccgtgcat gcagttgcag
     4261 tcgaaccagg tcgccgagcc ggccttgccg gtgaacagct tgatgccgta ctcggaggcc
     4321 atcttggtca ggccctcgtc ggacggcgtc cccgcgtcct gcatctgaag ggacttcttg
     4381 tagttgtcct tcggcgtggc ccccgcgcac ccgaggaacg tcttgtgcga ccccggcatg
     4441 atcatcagac cgccgttggt gtcgtagttc tccgtgagcg cgatcgagac cgagatcgtg
     4501 cgcatgttcg gcaggccgtc ctcggcgtgc caggtctcga agtccgagtg ccagtagaag
     4561 ccgctggccc cgaagcccgg cttgacgttg atccgcgact ggtggacgta gacgtccgag
     4621 ccgaggatct gccgggcccg accgaccacg cgctcgtcgc gcaccagttt cgcgaagacc
     4681 tcgctgatct tgtgcacctc gaagaccgac cggatctcct tggactgcgg ctccacgatc
     4741 gagcgctcgt ccgcgcggat ggccgggtcg ctggtgaggc gctccagctc ccgctggtac
     4801 tcggcgacct cgtcggggcc gatgagctgg tcgatggcga ggaagccgtc gcggtccagg
     4861 gcctggaggt cacccgccga gacgggaccg ggcgtgtccg gggagcccca gaccaccggg
     4921 tcctgccggg gggttgccac ctcggtggcg ccgcgggtgg gatagagatc agtgacgtgc
     4981 gtggtcgtgg tcacggtgac tcacacctcc tcggtgagca gcgggtagac gccgttctcg
     5041 tcgtgatcct cccgtccggt gacgggcggg ttgaagacac agatgcagtg gaagtcctcc
     5101 ttgaccttga gcgtgtgccg ctcgtggccg tcgaggagat acatcgtgcc cggcgtgatg
     5161 tggtacgtct tccccgtctc gcggtcggtc aactcggcgt cgcccttggt gcagacgacg
     5221 gcctcgatgt ggttcgcgta ccacatcgac gtttccgtac ccgcgtacag gatcgtctcg
     5281 tgcacggaga agccgacctt ctccttggcg aggacgatcc gggtgctttc ccaggtgccg
     5341 gacgcgctct tcacgtgccg gtcggtgcct tcgaactcct tgaacgaacg gacgatcacg
     5401 gtgttgcgat gcctcctagg tgagggtgtg caatggtccc cggcgccttc ggccgcgcgg
     5461 gccgaaggcg ccggaagtac gggtggtgcc gtgcgggtca ggcggtctcg cgcacggccc
     5521 gggccagggt cttcatgccc tcgtccagct cgtcgggcgt gatggtgagc gccggcagca
     5581 gcttgacgac ctcgctctcg ggcccggacg tctcgatgag cagccccagc tcgaaggcgc
     5641 gcttggcgac gcgtcccgcg cggtccttgt cgtggaactc caggccccag accaggccgc
     5701 ggccgcggta ctccttgacg tcggcgaggt tctcctcggt gatggcgatc atgtgctgct
     5761 cgacctgctc accgcgcttg cgggtctgct tctccatcgc cgacccgtcg gcccagtagg
     5821 cctccagcgt ggcggtcgcc gtgacgaacg ccgggttgtt gccgcggaag gtgccgttgt
     5881 gctcgcccgg ctcccagacg tccagctccg gcttgaacag ggtcagcgcc atcggcatgc
     5941 cgtagccgct gatcgacttg gagacggtga cgatgtccgg cgtgatgccc gcctcctcga
     6001 aggagaagaa ggcgccggta cggccgcagc ccatctggat gtcgtcgacg atgagcagca
     6061 tgtcctggcg ctcgcacagg gcggccaggg cgcgcagcca ctcggcccgg gcgacgttga
     6121 tgccaccctc gccctgcacg gtctcgacga tcacggcggc cggcttgttc aggccggagc
     6181 cctggtcctc gagcagccgc tcgaaccaga tgaagtcctc gacggtgccg tcgaagtagt
     6241 tgtcgaacgg catgggcgtg ccgtgcacca gcgggatgcc ggcgccggcc cgcttgaacg
     6301 cgttgccggt cacggcgagc gagcccagcg acatgccgtg gaaggcgttg gtgaaggaca
     6361 cgatcgactc gcgtcccttg accttgcgcg ccagcttcag cgcggcctcg acggcgttgg
     6421 tgcccgtcgg gcccgggaac atgaccttgt acggcaggtc gcgcttgcgc aggatggtgt
     6481 tctggaacgt ctccaggaag cgacgcttgg cggtggtcga catgtcgagc ccgtgcgtga
     6541 cgccgtcccg ctccaggtag tcgatcagcg cgcgtttcag cacggcgttg ttgtgcccgt
     6601 agttgagcga gcccgctccg gcgaagaagt cgaggtactc gtggccgtcc tcgtcgtaca
     6661 tgcggctgcc gaccgcacgg tcgaagacgg tgggccagcc gcggcagtag ctgcgcacct
     6721 cggactcgac ggtctcgaag acgctgaggt cgggctgggt gatggtcacg acgaatcgct
     6781 cctcgtgggg tgagggtggg agaggaaggt cgtacgggtc cggtgggacc ggtcgcggtc
     6841 ccggccggga agtctggggg ccggacggcc gggggtgccc cggcggctcg gcgaggggtc
     6901 agccgccggc ggacaggggg ccgatgcggt acaggacctc gggctcgtgc ggcccgtcgg
     6961 ggaacactgc cgtgtcgaac aggacttctc gctcgagccg ggcgccgcgg cgctcggcga
     7021 acgaggtgaa cagtcgttgg gacgccgtgt tgtcgggaga gatcgttgtc tccagggtgg
     7081 tgatcccgcg ttcgcgggcc acccgctccg ccagtccgtc gaccagcgtg gccgcgagcc
     7141 cacgaccacg gtacgtcccg tccaccgcca cctgccacac gagcagggtg tcggggctgt
     7201 ccggccgtac gtagccggtg atgaagccga ccggcacgcc gtgcccgtcg cggacgacgg
     7261 cagaggtggc ggcgaagtcc cggcaccaga gcaggtagct gtacgaggag ttcaggtcga
     7321 ggacctcgga atccctcgct atgcgccaca gcgcggctcc gtcggccact ctcggacggt
     7381 cgatttgcag gtctgcttgc gcggcagtca tgcaaattga atttacccag ggaaattcga
     7441 aattgcatcg ccggatgggg ttacgaatgg gcgcctcgtg tgttatcacg cgggcgcgtg
     7501 ccaccgcgcg aggcggcggc gctatgggat gatttgtggg ggatgcaagc cgcaaaacgg
     7561 gcagcatgtg gatcgggtca caatcgcgta acccgcgcga gacgtgtccg aaatgtgaag
     7621 atcggaactc gtgaaattgt tgtgtttagg tccgaggaaa gcgggcagaa gaatacggga
     7681 agctgcgctg agtggaattc cttaattctg cttgtgtgag catcgggaat tccctcgaat
     7741 tcatccttcc gacgggccgg taccggccgg ccgctcccac gcctcctcga cggcccgccg
     7801 ggccgcctcc ggatcggcct tcagaccccg ctcggccagc gcggcaccca gtgccgccag
     7861 gcagccccgc acggcctcgc gcgtcgcgtc cggaccgtag tggttgaccc ggatcatctc
     7921 gcccgccagc gcgcccccgc ccgccgccag cggcagcccg gggtcggact ccagagcgcg
     7981 cgccaccagc gcggacgcgt cgacgtccga cggcgtgcgc agcgtcgtcg cgaccggcgc
     8041 cgcgtccttc gcctcgtaca cgtacggctc cagaccgccc agcgcgagcg ccccggcccg
     8101 ggtggccgcc gcggcggacg cgtgccgcgc catcaccgtg ccggcgccct ccgcctcgat
     8161 gcgctccacg cacgcctcca gcgcgagcat ctccaactgc gccggcgcgt gcagcagcgc
     8221 cctgcgcccg ccgtcgatcc agcgctcctt ccagtccagg agggacaggt aggaccgtcg
     8281 cggcgcgttc gggttcgccg ccatccgcgc ccaggcccgc tcgctcaccg acaccgccga
     8341 caccccggcc gggccgccca tcgccttctg ggcgccgatc acgcacaggt ccacgcccca
     8401 cgcgtccggc agcaccggct ccgcgcccac cgaggcgacc gcgtccaggt agaagagcgc
     8461 gccgtgcgcc cgcaccacct cgccgatctc cgcgaccggg ttggtgttgc cggtcgccgc
     8521 ctcggcgtgc accagcgaca cgaagtcgat ctccgggtgc tcggcgaggg cctcgccgat
     8581 ccgctcggcg gagacggccg tgtggaacgg cacggcgagg tcgtgcacgg tcgcgccgca
     8641 gtcccgcagc cagttcccga aggtctgccc gtacgggccc gtgacgacgt tcagcgcgac
     8701 ggtgcccgga cccgcggcgg cgcggatcgc gccctccagc ggcagcagcg cctcgccctg
     8761 catgaccacc acgtcctgcc cggtgccgag caggcgcgcg acccggtcct cgatcgcggc
     8821 gaaacggccg gcgctcagcg gggcgagatc caggaaggga tgggtcacgg cggtgctctc
     8881 ttcagtgacg gcaggtgtac gacgcgggtg cgtcgcgcac ggacgcgcgc acgttcgtcc
     8941 gcacgcgcac acgtcccgta cggacgcggg tacgtcgcgt acggaatcga gagtaaccat
     9001 ggcgcccgcc ggcactcgcg ccggagggct tccgttgcga cacccccacg cccggaatca
     9061 tggcttccgt cacagccggg tgatcgttcg gctggtggaa tgggccccgt gctcgctctt
     9121 gtgctgctgg tcctcgtcgc ggcggcgacg gccgccgtcc tccgccgggg cgccggcacc
     9181 tacccccgcc accccgccgc ggtggccgcg gctcgcgtgc cacggctgcc gggcgcgccc
     9241 ttgaggggcg tcgccggggc cgccggcggg gccgccgccc tgctgtacct ctggggcctg
     9301 ctgtgcgtcg ggggcgcggt catggaagcg gaggacggcg gtacggactc cgtgccgccg
     9361 cggccgtgcc gtgacggggt gccgccggag cggtccgggc aggtcgtcga ctactccgtc
     9421 tcctacctgc ccctcggctt ccgctgcgag accctcgacg gcgacggcta cgactccgcc
     9481 gacatccccg gccacctcaa ccccggcgtc gccgtcctgg cgctcaccgc cgtcgggagc
     9541 gccgtggcgg ccgggtacgc gaccgagcgg cgcgcccgcg cggcggcgcg gagcgacggc
     9601 cgggcaccgg cctcgaaccg ccgtacgggc agcctttagg gtgcggggca tgagcgatcg
     9661 cgcggtgctg cacgtgaagg gtcgggtgct cgtcggaccg gaggaggtcc gggacgaggt
     9721 gtgggtgatc gacggccgga tctcctacga ccgccccgcg ggcgccgccg acgtccgcac
     9781 cgtcgagggg tgggcgctgc ccggcctggt cgacgcgcac tgccacgtcg gcctcgacca
     9841 gcacggcgcc gtccccgccg acgtcgccga gaagcagtcg ctgaccgacc gcgaggcggg
     9901 caccctgctc atccgcgacg cgggctcgcc ctccgacacc cgctggatcg acgaccgcga
     9961 ggacctcccg aagatcatcc gggccggccg ccacatcgcc cgcacccgcc gctacatccg
    10021 caactacgcc tgggaggtcg agcccgacga cctcgtcgcg tacgtcgccc aggaggcgcg
    10081 gcgcggcgac ggctgggtga agctggtcgg cgactggatc gaccgcgacc tcggcgacct
    10141 gtcggcctgc tggccgcgcg gcgccgtcga ggcggccatc gccgaggccc accgcctggg
    10201 cgcccgcgtc accgcgcact gcttcgccga ggactccctg cgcgacctgg tggaggccgg
    10261 gatcgactgc gtcgagcacg ccacgggact gaccgacgac acgatccccc tcttcgccga
    10321 gcgcggggtc gccatcgtgc ccaccctggt caacatcgcc accttccccg cgctggccga
    10381 cggcggcgag tcccgcttc
//
