LOCUS       NZ_WHZR01000001        10633 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces sp. OM5714 A912_AS4_SC1_R2, whole genome shotgun
            sequence.
ACCESSION   NZ_WHZR01000001
VERSION     NZ_WHZR01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. OM5714
  ORGANISM  Streptomyces sp. OM5714
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915160)
  AUTHORS   Samborskyy,M.
  TITLE     Whole Genome Sequence of the phthoxazolin producer Streptomyces sp.
            OM5714
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915160)
  AUTHORS   Samborskyy,M., Dickens,S. and Scott,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2019) Biochemistry, DNA Sequencing Facility,
            University of Cambridge, 80 Tennis Court Road, Cambridge,
            Cambridgeshire CB2 1GA, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2017
            Assembly Method                   :: Newbler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 102.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010993835.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:48:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,820
            CDSs (total)                      :: 7,730
            Genes (coding)                    :: 7,557
            CDSs (with protein)               :: 7,557
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 7, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 7, 5 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 58 of 173
            Pseudo Genes (incomplete)         :: 139 of 173
            Pseudo Genes (internal stop)      :: 18 of 173
            Pseudo Genes (multiple problems)  :: 36 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:03:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5341496
            Orig. end                         :: 5352129
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHZR01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10633
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="22"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10633
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10633
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5633](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5633
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(799..2142)
                     /locus_tag="STPH1_RS23665"
                     /old_locus_tag="STPH1_4700"
     CDS             complement(799..2142)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325465.1"
                     /locus_tag="STPH1_RS23665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_4700"
                     /product="glycosyltransferase"
                     /protein_id="WP_164384692.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 4.1e-32,
                     bitscore: 109.8, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLPPTGLRPRVLHLTQPVDGGVARVVTDLTRAQLATGLRVAVAC
                     PDGSLADRLRSLGADVRNWPATRSPGPALVPEVRRLVHVLEDVRPDLVHAHSAKAGLAG
                     RLAVRGRIPTVFQPHAWSFEAVGGAAGLLALGWERWAARWAARTVCVSEAERLRGVRAG
                     LRGPWSVVPNGIDLTRFDTGAPGEAPEDPGGVRLRHGIGPDAPLAVCVGRLCRQKGQDL
                     LLAAWETVRVRVPGARLVLVGDGPDRARLASRAPASVVFAGAVADATAWYRAADLVVLP
                     SRWEGMALAPLEAMACGRPVVVTDVDGAREGLPPALVPHCLVPPADPAALADAVAALLL
                     DAPLRASLGRRGRAHVRSAHDVRRTAAAVTALYDGLLYDGLPADVAPTADVAPTVGARP
                     TVGARPAVGVRSAAPARPGVAVDGTGRSAGPPAAARPLPIECRESTHP"
     misc_feature    complement(2119..2121)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2442..2681
                     /gene="chpG"
                     /locus_tag="STPH1_RS23670"
                     /old_locus_tag="STPH1_4701"
     CDS             2442..2681
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="STPH1_RS23670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_4701"
                     /product="chaplin ChpG"
                     /protein_id="WP_007449696.1"
                     /transl_table=11
                     /translation="MSRIAKGLALTSVAAAAVAGTAGVAAADSGAQAAAAHSPGVLSGN
                     VVQVPVHIPVNVCGNTIDVIGLLNPAFGNECEND"
     gene            2956..3720
                     /locus_tag="STPH1_RS23675"
                     /old_locus_tag="STPH1_4702"
     CDS             2956..3720
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325463.1"
                     /locus_tag="STPH1_RS23675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_4702"
                     /product="hypothetical protein"
                     /protein_id="WP_164384693.1"
                     /transl_table=11
                     /translation="MRLPARRIATSTLCAGLLIGLSGPAVMAADGDPVRERTHAASHAP
                     LPDADELQSQVAGLADLGGVLTPVTDMLTAVLKADNGQLSATDADKLSAAVEDALAKAD
                     AADTDADDTATTPSTTTPGTTTPAEPPAVTAPEAGTDTPVTLPAPVTAQSDDGTAAASD
                     LSAAAEAALQKQIDALVKATTSGTAEQVSPAVKDVMTGIVNVVAATLVGNGLPAADLAG
                     LPGVPTAPEATTPEATTPEAAAPANPLPADPS"
     gene            3940..4242
                     /locus_tag="STPH1_RS23680"
                     /old_locus_tag="STPH1_4703"
     CDS             3940..4242
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445910.1"
                     /locus_tag="STPH1_RS23680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_4703"
                     /product="hypothetical protein"
                     /protein_id="WP_153179802.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLVAAGSAVPAFAYDAADTAALTPTSLNGAVNA
                     LTAEPIDIMPIKHQSDARSDALDTENKDSVLHGVKGATTELNSAGGLLGGLPLGG"
     gene            4359..4874
                     /locus_tag="STPH1_RS23685"
                     /old_locus_tag="STPH1_4704"
     CDS             4359..4874
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="STPH1_RS23685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_4704"
                     /product="hypothetical protein"
                     /protein_id="WP_164384694.1"
                     /transl_table=11
                     /translation="MKPTTRRTLAAVVTGVAAAVGATATPAVAARAIPVPVPLDGAETA
                     LGMELPEVAGELPVPTAGAPEGPRYVEGRLLPERTLPQLPVRGGLPGVDLRQPVPHVLG
                     DDFDHAGVDAPAAELRTLAPGLSLDAPLTAPNPDALGLPAPKLPEAGVLAPVVRTAPVA
                     DLGVGPGL"
     gene            5001..5633
                     /locus_tag="STPH1_RS23690"
                     /old_locus_tag="STPH1_4705"
     CDS             5001..5633
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028525.1"
                     /locus_tag="STPH1_RS23690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_4705"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_164384695.1"
                     /sec_met_domain="melC (E-value: 6.1e-50, bitscore: 167.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVNAGTRTNGTEGERRHAAQGEGAERGRPAKPGQAAPGPVNPGQ
                     VNPGPASGTRRQVMRGLLAPALAVGLAPLVAASRPARATEAAADAGDAAGPAHPGPHAA
                     GSAPLTADSTAFDETYRGRRIRGIRSAAGRAVGAGTWHVTVDGRPLHLMRRADGSWLSM
                     IDHYRSYPTPLAAARGAVDELGPGEHLRDTPSTGHDQHPGGRHGVHA"
     gene            5617..6483
                     /locus_tag="STPH1_RS23695"
                     /old_locus_tag="STPH1_4706"
     CDS             5617..6483
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="STPH1_RS23695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_4706"
                     /product="tyrosinase family protein"
                     /protein_id="WP_007449722.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTRSEKRRFVNALLEIKRRGEYDEFVRTHIEYYVS
                     DGEDGLRTAHMAPSFLPWHRRFLLDLEEALRRVDSSVTVPYWDWTKDRGAKSTPWTADL
                     LGGTGRRSDHRVTTGPFAHAEGNWTIKENVTDTEYLTRDLGRAGNPLDLPSKSDLEWAL
                     DDPKYDTSPYDSTVRKGFRNKLEGWGTGRGSVSWRNHNRVHRWVGGAMVGGASVNDPVF
                     WLHHAFIDLQWSRWQARHRGARYLPAEPPGRGSAQRGRIVARHEKLPPWDVTPDELEDV
                     GRIYRYA"
     gene            complement(6546..6818)
                     /gene="chpF"
                     /locus_tag="STPH1_RS23700"
                     /old_locus_tag="STPH1_4707"
     CDS             complement(6546..6818)
                     /codon_start=1
                     /gene="chpF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028524.1"
                     /locus_tag="STPH1_RS23700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_4707"
                     /product="chaplin ChpF"
                     /protein_id="WP_087790917.1"
                     /transl_table=11
                     /translation="MSRIAKAAGVALGAGAVVLSGTGMAMADAGAEGAAIGSPGVLSGN
                     VVQVPVHVPVNVCGNTIDVIGLLNPAFGNACENGDDDEKSGGYGG"
     misc_feature    6919..6934
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.82"
                     /tool="antismash"
     gene            7008..7643
                     /locus_tag="STPH1_RS23705"
                     /old_locus_tag="STPH1_4708"
     CDS             7008..7643
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449721.1"
                     /locus_tag="STPH1_RS23705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_4708"
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_164384696.1"
                     /transl_table=11
                     /translation="MPNDRSDASAGAPEPSGPTPSSALAALPALFSEESRVRAFAAVAL
                     GAGDAARVARAAGLSTKDTAGALRRLRDQGVVTSGDDGELVVAHDRFRELARAARPAPS
                     AAGSSAVEGDDGPAGIVVRTFVRDGRLVRLPARWTRKKVVLRHIAEQTFAPGTEYPERT
                     VDEKLRAWCEDGEVDHVTLRRHLVDLGHLRRSDGVYSRPADPARTGTA"
     gene            complement(7651..8622)
                     /locus_tag="STPH1_RS23710"
                     /old_locus_tag="STPH1_4709"
     CDS             complement(7651..8622)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449723.1"
                     /locus_tag="STPH1_RS23710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_4709"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_164384697.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 2.8e-51, bitscore:
                     172.0, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQQVATEIADWSPLAGEVDVVSYDTHFPDEDALAA
                     ALADVDIVVTLRERVPFPGSLIARLPRLKLIVASGMRNSVIDYAAAEAHGVTVCGTASS
                     STPPVELTWALLLGLARGIVQEGNGLREGGPWQRTVGADLHGRRLGLLGLGKIGGRVAQ
                     VGLAFGMRVSAWSQNLTRERTDEVGVELARSKEELLSTADFVSVHLALGERTRGLLGPA
                     ELALLKPTAYLVNTSRAAIVDQEALLAALHEGRIAGAGVDVFDTEPLPAGHPMRTAPRL
                     LATPHLGYVSRANYATYYGQAVEAIGAYLAGSPVRRLPVSPA"
     tRNA            complement(8653..8728)
                     /anticodon=(pos:complement(5350189..5350191),aa:Arg,
                     seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="STPH1_RS23715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="STPH1_t050"
                     /product="tRNA-Arg"
     gene            complement(8653..8728)
                     /locus_tag="STPH1_RS23715"
                     /old_locus_tag="STPH1_t050"
     misc_feature    9001..9016
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.68"
                     /tool="antismash"
     misc_feature    9003..9017
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.51"
                     /tool="antismash"
     misc_feature    complement(9004..9019)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.93"
                     /tool="antismash"
     misc_feature    complement(9006..9019)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.26"
                     /tool="antismash"
     misc_feature    complement(9028..9042)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.23"
                     /tool="antismash"
     misc_feature    9031..9047
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.29"
                     /tool="antismash"
ORIGIN
        1 ccgcgtaccg gggggccggg tcggcggtct cggcgtcgag ctcccacacg gtgcagcccg
       61 actcggccag cgcccgcagc aacgccgcct gccggggccg cggggacggg tccacggcca
      121 ggacgtcccg gacgccgttc tgcacgaccg cccgccgcac ctcctcgccc gtggacagca
      181 cgggcagacc cgcggtgccg cccgggccgt cggtgacgat cccgacgggg cgcatcccgc
      241 agcgcgggtg gcgcagcagc gccgaggcca cgcgctgcgc ggtcgcggcg ggcccgacga
      301 cgagggcggc gcgggggcgg ttcagcagcg ccgtacggtg ccggtggtgc acggcggccc
      361 ggccggcgca gcccgtcacg gcgtgcagca ggaaacccgc ggccagggtg tgcgcgggga
      421 gcgcgtgcgc cggggtgtac gccgcgacga gcgccgccag caccagccac accaccgcga
      481 tccggccgca cagggcgggc agctcgtcca gcgcgccgga cggccgccgg tccgggcgtg
      541 ggcgcagcag cagcgaggcg gccaccagca gcgccgcgag cagcggccgg tgcgggacac
      601 cggtgaggac cagcgagccc ggcagggccg ccgtgccgtc cgcgacgagc aacggcaacg
      661 gcgagcccgg ccgcggtgcg ggaggcccgg cgggcagccg gtggctcccg gtgccgtcgc
      721 gccgcggcag caccgcgacg ggggaggagc cgaggtcgcg cgactggccg gcgggggagg
      781 gaacggtgct ttccgcggtc acgggtgggt ggactccctg cactcgatgg ggagggggcg
      841 cgcagcggcg ggcgggccgg cgctcctgcc ggtgccgtcg acggcgacgc cggggcgcgc
      901 cggggccgcg gaccgcacgc cgacggcggg ccgtgcgccg acggtggggc gtgcgccgac
      961 ggtgggtgcc acgtcggcgg tgggtgccac gtcggccggg aggccgtcgt aaaggaggcc
     1021 gtcgtaaagg gccgttaccg ccgcggccgt gcgccgtacg tcgtgcgcgg accggacgtg
     1081 cgcgcgcccc cggcggccga gcgacgcgcg cagcggcgcg tcgagcagca gcgcggcgac
     1141 ggcgtcggcc agggccgccg gatccgcggg cggtaccagg cagtgcggta cgagagcggg
     1201 cggcaggccc tcgcgggcac cgtccacgtc ggtcaccacc acggggcggc cgcacgccat
     1261 cgcctccagc ggggccagcg ccatgccctc ccagcgcgac ggcaggacga ccaggtcggc
     1321 ggcccggtac caggcggtgg cgtcggcgac ggctcccgcg aagaccacgg aggccggggc
     1381 ccgcgaggcc agccgggcgc ggtcagggcc gtcgccgacg agcaccagcc gggcgccggg
     1441 cacccggacc cgcaccgttt cccacgcggc caggaggagg tcctgcccct tctgccggca
     1501 caggcgcccc acgcagaccg ccaacggcgc gtccggaccg atgccgtgcc gcagccgtac
     1561 gccgcccggg tcctcgggag cttccccggg cgcgccggtg tcgaagcggg tgaggtcgat
     1621 gccgttcggg accacggacc agggcccgcg cagccccgcc cgcacacccc gcagccgttc
     1681 cgcctcgctc acgcacaccg tccgcgccgc ccaccgcgcc gcccaccgct cccagccgag
     1741 cgcgaggagc ccggcggcac cgccgaccgc ctcgaacgac caggcgtgcg gctggaacac
     1801 ggtcggaatc cggccccgta cggcgagccg ccccgcgaga ccggccttcg cgctgtgcgc
     1861 gtgcaccagg tcgggccgta cgtcctcgag cacgtggacg agccgccgca cctccggcac
     1921 cagcgccggg cccggcgaac gcgtcgcggg ccagttccgc acatcggcgc ccagggaacg
     1981 cagccggtcg gcgaggctcc cgtcgggaca ggcgacggcg acgcgcagcc cggtggcgag
     2041 ctgagcgcgc gtcaggtccg tcaccacccg ggcgaccccg ccgtccaccg gttgggtgag
     2101 atgcagaacc cgtggccgta agccggtcgg tggcaggtgc atgcgcgttt cctcgctcac
     2161 ggggaatgct cggtacgggc gggtacgcgc ccggccgcgg gcgccggccg cgacgcggca
     2221 tacgggtgcg tcgaaacggt cgaaacgggt gcgtcgaatg gtgtgggact ctagctgcta
     2281 tccgtactaa tacggcgaaa ccgggtaagt gtgttggatt tgtgaatgcc ggcctttcgc
     2341 gagatcaccc ctttggcggc acaactcgga cgcgcgccac gcgttgacac tgcgccgggc
     2401 agccgcccgg atgtgtgtac aaccccaagg agcacttctc catgtcgcgt atcgcgaagg
     2461 gcctggccct gacctccgtc gccgccgccg cggtggcggg caccgccggt gtcgccgccg
     2521 ccgacagcgg cgcgcaggcc gccgccgccc actccccggg cgtcctgtcc ggcaacgtgg
     2581 tgcaggtccc ggtccacatc ccggtcaacg tctgcggtaa caccatcgac gtcatcggtc
     2641 tgctgaaccc ggcgttcggc aacgagtgcg agaacgactg acgtcggctg gcaactcccc
     2701 gcaccggccg tccgcaccgc ggttcctccg cgagcgggcg gccggttcgc gttgccgcgc
     2761 ggacggcggt gtgctgcccc gaatgggggg accgggccgg gacacggcgc acggaccttg
     2821 acagggcgtc tcagcaggta agacgccgca cgcggggaga gcgggcggac cggccgggtc
     2881 accgctcggt tgcagagcgc caagggcact tccacggtgg gcacaacatc cgacgcacca
     2941 ccgaaaggaa cccccatgcg tctgcccgca cggcgaatcg ccacctccac cctctgcgcc
     3001 ggcctgctga tcggcctctc cggtccggcg gtgatggcgg ccgacggcga cccggtccgg
     3061 gagcgcaccc acgcggcgtc ccacgccccc ctccccgacg ccgacgaact gcagagccag
     3121 gtcgccggcc tggccgacct gggcggggta ctcacgccgg tcaccgacat gctcaccgcc
     3181 gtcctcaagg ccgacaacgg ccaactctcc gccacggacg cggacaagct ctccgccgcc
     3241 gtcgaggacg ccctcgccaa ggcggacgcc gcggacacgg acgccgacga cacggcgacc
     3301 actcccagca cgaccacccc cggcacgacc accccggccg agccgccggc cgtgaccgcg
     3361 ccggaggccg ggaccgacac gccggtcacg ctgccggcgc ccgtcacggc gcagagcgac
     3421 gacggcaccg cggccgcctc cgacctgtcc gcggccgcgg aggccgcgct gcagaagcag
     3481 atcgacgccc tggtcaaggc caccacctcc ggtaccgccg aacaggtgag cccggccgtc
     3541 aaggacgtga tgaccggcat cgtcaacgtc gtcgcggcca cgctggtggg caacgggctg
     3601 cccgccgcgg acctggccgg cctgcccggc gttcccaccg cgcccgaggc cacgaccccc
     3661 gaggcgacca cgccggaagc cgccgcgccc gcgaacccgc tgccggccga cccctcctga
     3721 cgccgccccc tcccgggcgt cccgggccgt cgtacctccc tccccggagg cgcgacggcc
     3781 ctttcccgtg tcggatcccg tgcgtcgggt cccgtctccg gagatctcat atgtcttccg
     3841 gtgtcttccg gacggcggag tttccgggtc cggcgcaggt cgttagggag ggcgtcagca
     3901 atcccaccgg tgacacgggt cgccgaggaa aggaacacga tgaagtccct caaggccgcc
     3961 gccgtcgtcg ccggttccct ggtcgccgca ggctccgccg tgcccgcgtt cgcctacgac
     4021 gccgccgaca ccgccgctct cacgcccacc agcctcaacg gcgccgtgaa cgcgctgacc
     4081 gcggagccca tcgacatcat gccgatcaag caccagtcgg acgcgcggtc cgacgcgctg
     4141 gacaccgaga acaaggactc cgtgctgcac ggtgtgaagg gcgcgacgac cgaactgaac
     4201 tccgccggtg gcctgctggg cggcctcccg ctgggcggct gagccacccc gctccccgcc
     4261 gcgctcctcc ctcctccctc ctccctcctc cctccccctc cctctcccgc cttcccgaag
     4321 ccgtacttcc cgccttctcg aaagggaccg tgcctgtcat gaagccgacc accagacgaa
     4381 ccctggccgc cgtcgtcacc ggtgtggccg ccgccgtggg cgcgaccgcc actcccgccg
     4441 tcgctgcccg cgcgattccc gtaccggtgc cgctggacgg cgcggagacg gccctcggca
     4501 tggaactgcc cgaggtggcc ggcgagctgc ccgtgccgac ggcgggggcc ccagagggcc
     4561 cgcggtacgt ggagggccgg ctgctgccgg aacgcacgct gccgcaactg ccggtgcgcg
     4621 gcgggctgcc cggcgtcgac ctgcgccagc cggtcccgca cgtcctcggc gacgacttcg
     4681 accacgcggg tgtcgacgcg cccgccgccg aactgcggac cctggcgccg ggcctgtccc
     4741 tggacgcgcc gctcaccgcg ccgaaccccg acgccctggg cctgccggcg cccaagctcc
     4801 ccgaggccgg ggtgctcgcc cccgtggtcc ggacggcgcc ggtggccgac ctgggtgtgg
     4861 gaccggggct gtaggcgttc cggcccgaag gggcctcgcc cggtggagcg gccgtgctcc
     4921 gactggccct gcggcggtga ccgcgaggcc cgccccgcgg ttagcggtgc ggaccaacgc
     4981 aaccggacga ggagtgggcc atggccgtca acgcgggcac cagaacgaac ggcacggagg
     5041 gggagcggcg gcacgcggcg caaggggagg gcgcggagcg aggccgaccg gcgaagcccg
     5101 ggcaggcggc ccccggaccg gtgaaccccg ggcaggtgaa ccccggcccg gcgtccggca
     5161 cccggcggca ggtgatgcgg gggctgctcg ccccggcgct cgccgtcggc ctggcgcccc
     5221 tggtcgccgc ctcccggccc gcccgggcca cggaggccgc cgccgacgcc ggggacgcgg
     5281 cgggacccgc acaccccggg ccgcacgccg ccggatccgc ccccctgacc gccgacagca
     5341 ccgccttcga cgagacctac cgcggccggc gcatccgggg catccgctcc gccgcgggac
     5401 gggccgtggg cgccgggacc tggcacgtca ccgtggacgg ccgcccgctg cacctgatgc
     5461 gccgggccga cggcagctgg ctgagcatga tcgaccacta ccggtcgtac cccaccccgc
     5521 tcgccgcggc ccgcggcgcc gtcgacgaac tcggccccgg cgagcatctg cgcgacaccc
     5581 cgtccaccgg ccacgaccag caccccgggg ggcggcatgg cgtacacgcg taaggacgtc
     5641 agcacactga cccggagcga gaagcgacgg ttcgtcaacg cgctgctgga gatcaaacga
     5701 cgcggggagt acgacgagtt cgtgcgcacc cacatcgagt actacgtctc cgacggcgag
     5761 gacggcctgc gtacggcgca catggcgccg tccttcctgc cctggcaccg ccggttcctg
     5821 ctggacctgg aggaggcgct gcgccgggtc gactcgtcgg tcaccgtgcc gtactgggac
     5881 tggacgaagg accgcggcgc caagtcgacg ccctggaccg ccgacctgct gggcggcacc
     5941 ggacggcggt ccgaccaccg ggtcaccacc ggccccttcg cccatgccga gggcaactgg
     6001 accatcaagg agaacgtcac cgacaccgag tacctcaccc gggacctcgg acgcgccggc
     6061 aaccccctcg acctgccctc caagagcgac ctggaatggg cgctggacga cccgaagtac
     6121 gacacctccc cgtacgactc cacggtccgc aaggggttcc gcaacaagct ggaggggtgg
     6181 ggcaccggcc gcggcagcgt ctcctggcgc aaccacaacc gcgtgcaccg ctgggtcggc
     6241 ggcgccatgg tcggcggcgc ctccgtcaac gacccggtgt tctggctgca ccatgcgttc
     6301 atcgacctcc agtggtcccg ctggcaggcc cgccaccgcg gtgcccgcta cctgcccgcc
     6361 gagccgcccg gccggggcag cgcgcagcgc gggcggatcg tcgcacggca cgagaagctg
     6421 ccgccgtggg acgtgacgcc ggacgagctg gaggacgtcg gccggatcta ccggtacgcc
     6481 taggcggtgc cctcacggca ggagccccgg ccgggaaggc cggggctcct cgggtgcctg
     6541 gcgactcagc cgccgtagcc gccgctcttc tcgtcgtcgt cgccgttttc gcaggcgttg
     6601 ccgaaggccg ggttcagcag gccgatcacg tcgatggtgt tgccgcagac gttgaccggc
     6661 acgtgcaccg ggacctggac gacgttgccg gacaggacac cgggcgagcc gatggccgcg
     6721 ccctccgcgc ccgcgtccgc catggccatg ccggtgccgc tgagcaccac ggcaccggcg
     6781 ccgagggcga caccggcggc cttcgcgatg cgagacatca cgttctcctt ctgactcggg
     6841 gagcgcggcg gcaggacgcg ccgccgcact gccggttcaa cgcacccggt cgcagccggt
     6901 cacgggggga atgtgcggat cacccttttt ggacgctcgg gccggtgcgg aggcgggccg
     6961 tggaaaaccg ttcccgtgcg cgccggtgtg tccactagcc tccgggcatg ccgaatgacc
     7021 gatccgacgc gtccgccggg gctcccgagc cgtccggacc gaccccgtcc tccgcgctcg
     7081 ccgcgctgcc cgccctcttc tccgaggaga gccgggtgcg ggccttcgcg gccgtggcac
     7141 tgggcgcggg cgacgccgcg cgggtggccc gggcggccgg cctctccacg aaggacacgg
     7201 ccggtgcgct gcgccggctg cgcgaccagg gcgtggtcac gtccggcgac gacggtgaac
     7261 tcgtcgtcgc ccacgaccgg ttccgggagc tggcccgcgc cgcccgtccg gcgccgtcgg
     7321 cggccgggtc ctccgccgtg gagggtgacg acggcccggc gggcatcgtg gtgcggacct
     7381 tcgtccggga cggccggctg gtccggctgc ccgcgcgctg gacgcgcaag aaggtggtgc
     7441 tgcggcacat cgccgagcag accttcgcgc ccggcacgga gtacccggag cgcaccgtcg
     7501 acgagaagct gcgtgcctgg tgcgaggacg gcgaggtcga ccatgtgacg ctccggcgcc
     7561 acctggtgga cctcggtcac ctgcgccgga gcgacggcgt ctactcccgc ccggccgacc
     7621 cggcccggac gggcaccgcc tgaacggccc ctacgcgggg gacacgggca gccgccgtac
     7681 gggggagccc gcgaggtagg cgccgatcgc ctcgacggcc tggccgtagt acgtggcgta
     7741 gttggcgcgc gacacatagc ccaggtgggg tgtggcgagc aggcgcgggg cggtgcgcat
     7801 cgggtggccg gcgggcagcg gctccgtgtc gaagacgtcc acgcccgcgc cggcgatgcg
     7861 gccctcgtgc agcgcggcga gcagcgcctc ctggtcgacg atcgccgcgc gcgaggtgtt
     7921 gaccaggtag gcggtcggct tgagcagggc cagttcggcg gggccgagca ggccgcgggt
     7981 gcgctcgccc agcgccaggt ggacggagac gaagtcggcg gtgctcagga gctcttcctt
     8041 ggagcgggcc agctcgacgc cgacctcgtc ggtgcgctcc cgggtgaggt tctggctcca
     8101 ggcgctcacc cgcatgccga aggccaggcc gacctgtgcc acccgtccgc cgatcttccc
     8161 caggcccagc agtccgagcc ggcggccgtg caggtcggcg cccaccgtcc gctgccaggg
     8221 gccgccctcg cgcagcccgt tgccctcctg gacgatgccg cgggccaggc cgagcagcag
     8281 ggcccaggtc agctccacgg gcggggtgga ggaggaggcc gtgccgcata cggtgacgcc
     8341 gtgcgcctcg gcggcggcgt agtcgatcac gctgttgcgc atgccggagg cgacgatcag
     8401 tttcagccgg ggcagccggg cgatcagcga gccggggaag ggcacgcgtt cgcgcagggt
     8461 gaccacgatg tcgacgtcgg cgagcgccgc ggcgagcgcg tcctcgtccg ggaagtgggt
     8521 gtcgtaggag acgacgtcca cctcgcccgc gagcggggac cagtcggcga tctcggtcgc
     8581 cacctgctgg aagtcgtcga gcaccgcgca gcgcagtcgc acgtcgtccg gtccctcccg
     8641 ccaaacgagc catggcgccc ccggcaggac tcgaacctgc ggccaagcgc ttagaaggcg
     8701 cctgctctat ccactgagct acgggggcca ggtgtggtgg cctcggtcgt ggggtccggg
     8761 catggccggt ccgtgacgtc gccggggaca aggatagggc tcccggctcc atgtccctga
     8821 tgcttcccct ccgtggctcg atgtggaggt tcggtgaagc ggtcctgata atcgcaggca
     8881 gttacgaatc gtgcatcgct tttggcgtct cgcgcatcgg gtgttgtcca ctcgttatgc
     8941 ctggactgtc ctcgtgtccc ggtagtccct tccgtccgga gtgtccggtc ggcgcgcgga
     9001 catgctcatc atgctcatat gcttcagaat tccctcaaaa ttgggcattc ttcccatgtg
     9061 gtgaccttgg acgtacggcc tcagctgctc gacgcactct ccgccctgcg cgaccgtgtc
     9121 gcggccgcac gcttcccgct gcccctggcg ggggcgccac gcgcgcgtgc caaccgcgac
     9181 gaactgctcg cacagctcga cgactacttg gtgccccgcc tcaaagagcc cgaagcgccg
     9241 ttgctggccg tcgtgggcgg atccaccgga gcgggcaagt cgaccctggt gaactccctc
     9301 gtggggcgcc gggtcagcga ggcgggtgtg ctgcggccca cgacccgcac accggtcctg
     9361 gtctgccacc cggaggacca ccactggttc agcggcatgc gggtgctgcc cgacctcacg
     9421 cgcgtgtggg tgccccaccg ggacccggac gacgacctgc tgctgcccgg cgagaacccc
     9481 gcgcgcgtgc tgcgggtcga gaccgccgac accctgccgc ccggcatcgc cctcctggac
     9541 gcgcccgaca tcgactccct cgtcgccgac aaccgcgtcc tggcggccga actgatctgc
     9601 gccgccgaca tctgggtgat ggtcaccacg gccgcccggt acgccgacgc ggtgccctgg
     9661 cacctgctgc gcaccgccaa ggagtacgac gcgaccctgg tcaccgtgct cgaccgggtg
     9721 ccccaccagg tggtcgccga ggtctcgcgg cagtacgggg cgctgctcac caaggccgga
     9781 ctcggcgagg tcccccgctt caccgtcccc gaactgcccg agtcggcctg gggcggcggg
     9841 ctgctgccgg ccagcgccgt ggcacccctg cggacctggc tcgtccaccg cgcccaggaa
     9901 ctcgaggccc ggcaccacgc catggcccgt acggcgcacg gcatcctgga ctcgctgaag
     9961 tcgcggatgc ccgagctggc cggcgccacc gccgcgcagt acgccgccgc gctgcggctc
    10021 accaccgccg tcgacggcgc ctacgacggc gagcacgcgc gcgtgcgggg gcggttgcgg
    10081 gcgggcgccg tactcgccgg ggacgcgctc aagcgctggc gggccttccc cctcgactgc
    10141 tccccggaag agctcctcga ctcgctggtg gaaagcctga gcgccctgct gctgtgcgcc
    10201 gtcacggccg ccgacgaaca cgtcgacgag gcctggcgcc gggaaccggc ggcggacgcc
    10261 cccgaactcg ccgcccacgc ccccgcgccg gacagcgccg agcaccgcat cgggatggcc
    10321 gtacggcggt ggcggcgcga gctggaggag tacgccgagg aggaggtgcg cgagctggac
    10381 cggaacctcg cgccggaccc cggggtggtg gccggcctgg tcgccaccgt gctgctgggc
    10441 ggccggcgcg cgcgtgccgc cggggagcgg ctcgccgaac ggatcggcgc ccacggcgcg
    10501 ctgcgcctgc gcgaccgggg cggccggctg ctcgacgaac acgtcgaccg ggtcatgcac
    10561 gccgaacgcg agcgcaggct ggctccgctc gacggcctgg aggtgcaccc cgagccacag
    10621 gcggagctga tcg
//
