LOCUS       NZ_WHZR01000001        11386 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces sp. OM5714 A912_AS4_SC1_R2, whole genome shotgun
            sequence.
ACCESSION   NZ_WHZR01000001
VERSION     NZ_WHZR01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. OM5714
  ORGANISM  Streptomyces sp. OM5714
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915160)
  AUTHORS   Samborskyy,M.
  TITLE     Whole Genome Sequence of the phthoxazolin producer Streptomyces sp.
            OM5714
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915160)
  AUTHORS   Samborskyy,M., Dickens,S. and Scott,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2019) Biochemistry, DNA Sequencing Facility,
            University of Cambridge, 80 Tennis Court Road, Cambridge,
            Cambridgeshire CB2 1GA, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2017
            Assembly Method                   :: Newbler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 102.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010993835.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:48:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,820
            CDSs (total)                      :: 7,730
            Genes (coding)                    :: 7,557
            CDSs (with protein)               :: 7,557
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 7, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 7, 5 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 58 of 173
            Pseudo Genes (incomplete)         :: 139 of 173
            Pseudo Genes (internal stop)      :: 18 of 173
            Pseudo Genes (multiple problems)  :: 36 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:03:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1646616
            Orig. end                         :: 1658002
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHZR01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11386
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="12"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11386
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11386
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6386](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6386
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    596..610
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 15.82"
                     /tool="antismash"
     misc_feature    1196..1211
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.16"
                     /tool="antismash"
     gene            1417..2295
                     /locus_tag="STPH1_RS07055"
                     /old_locus_tag="STPH1_1413"
     CDS             1417..2295
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445327.1"
                     /locus_tag="STPH1_RS07055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1413"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_164382544.1"
                     /transl_table=11
                     /translation="MISLVRRTTALCVLGAALAACGTPDAGQTPRPAPSAPAPSSSPSP
                     SRTPTLAPGPAGLTPVFEHGPRTDGAKDGRKNGKTVALTFDADMTADQGPRAASGERFD
                     NPQLIATLRRLKVPATVFMTGRWAEEYPDQARSLGRDPGFEIANHSYSHYAYTDDCYGL
                     PTVSKDDMRTDLERAYAAFEKAGVPNPMPYFRFPGGCYDKAALKELSATGVTAVQWDVV
                     SGDAFATDADAVTRQVLDGVRPGSVVVMHCTRSAAPVTEEVVRAVVPELRRRGYRFVKV
                     SELIGAANGRG"
     misc_feature    2316..2334
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.14"
                     /tool="antismash"
     gene            2326..2553
                     /locus_tag="STPH1_RS07060"
                     /old_locus_tag="STPH1_1414"
     CDS             2326..2553
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972872.1"
                     /locus_tag="STPH1_RS07060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1414"
                     /product="hypothetical protein"
                     /protein_id="WP_108987611.1"
                     /transl_table=11
                     /translation="MTSDHDQGPDGSGSGCEPGGYSLIDSAPRPPRADGPPYADCVVCR
                     EPTEYPESYKGITLCPVCEWQEAQRTACSG"
     gene            complement(2545..3219)
                     /locus_tag="STPH1_RS07065"
                     /old_locus_tag="STPH1_1415"
     CDS             complement(2545..3219)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972873.1"
                     /locus_tag="STPH1_RS07065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1415"
                     /product="ABC transporter"
                     /protein_id="WP_164382547.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLLRSQRWLPPVVLYAAALGVGVQGGQPVLDSLGW
                     AAAVLLPVAAWLVRICVTGEPSAARACVSAARGPARAHLASVLVALAAATVLGAVATVV
                     VTLISDPVSTGHGTRVPLLPAGGAGLLAALVCALLGTAVGALTAWPVLRSPGRAVPALL
                     LAALLALVASGSPARAAVSGLVTGSKSGAVPLPLLPLAGAAVVAAAATAAACALSSRRT
                     SA"
     gene            complement(3216..4070)
                     /locus_tag="STPH1_RS07070"
                     /old_locus_tag="STPH1_1416"
     CDS             complement(3216..4070)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972874.1"
                     /locus_tag="STPH1_RS07070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1416"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_164382548.1"
                     /transl_table=11
                     /translation="MQRELRLDGVGRRYGIRGPWVLRGVDLGVPSGTLVRVEGTNGTGK
                     STLLRLLAGIDAPTEGRVTGRPRTAYVPERFPPALPFTAAGYLTHLGTVHGLSRRAAAR
                     AAGQWLERLGAAGYAGTRLSRLSKGGSQKVAVAQALVAEPELLVLDEAWTGLDAGARGE
                     LERAVAERVAGGGAVVFVDHDPGRLAGAPDAVYTVRDGTLHRRTGPAPAAAGPRVVVRV
                     QGPPGAALPADAYRTAASVEETGPGSHTIGVPASHSDVLLRTLLTARPPWHVVSVHAPE
                     DPR"
     gene            4138..4902
                     /locus_tag="STPH1_RS07075"
                     /old_locus_tag="STPH1_1417"
     CDS             4138..4902
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445276.1"
                     /locus_tag="STPH1_RS07075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1417"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_164382550.1"
                     /transl_table=11
                     /translation="MSNDPTTPVPASVPAAAPASAPTPDSPFRAEPGDRDLAPQFVLPL
                     VVRIERAAPPPRTDALETAARAVLVMLGDERSTGDGAWARAMRDWQDARIRKVVRRARG
                     AEWRRAEALPGITVTGKSAEVRVFPPVPLDGWPKDLARLQVSGTDLDDLEPPAAADPAA
                     PVLWMNPDLDMSAGKAMAQAGHGAQLAWWALSDGERSAWREAGFPLSVRTADPARWGGL
                     TTGGLPVVRDAGFTEIAPGSCTVVADHPALRR"
     gene            5001..6386
                     /locus_tag="STPH1_RS07080"
                     /old_locus_tag="STPH1_1418"
     CDS             5001..6386
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445275.1"
                     /locus_tag="STPH1_RS07080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1418"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_164382552.1"
                     /sec_met_domain="DUF692 (E-value: 2.8e-99, bitscore: 330.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERTARLGTGIGWRPEIADAVERMPGIDWVEAVSENLCPGHLPDS
                     LLRLRERGVTVVPHGVSLGLGGAERPDAGRLAALAERAEVLGAPLVTEHIAFVRAGGAL
                     TASPPLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALVAWPDEELTEGQ
                     FLYELADRTGVRLLIDVANLHTNHVNLGADPAEALAELPLEALAYVHVAGGFERDGVWH
                     DSHAHPVAQPVLDILTDLASRVAPPGVLLERDENFPDGDGLSGEVEAIRTAVAKGRAGS
                     PGAVTGGAAPRTSGAGSGSRSGCRSGSGSGSGSGSGSGSTGDAVRQRLGLAQAALLSAL
                     VAGTPVPEGFDRVRLGVQARALAGKRADVVGKVAPELPVILGAGYRPAFLAYAQGSPLT
                     GGYRRDALDFAAHVLRTAPGDEDPAVRRALREWWLDRSGPVPRSRRPGARLARATRRVL
                     LRR"
     misc_feature    complement(6425..6442)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.84"
                     /tool="antismash"
     misc_feature    complement(6485..6498)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.43"
                     /tool="antismash"
     gene            6503..7300
                     /locus_tag="STPH1_RS07085"
                     /old_locus_tag="STPH1_1419"
     CDS             6503..7300
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972877.1"
                     /locus_tag="STPH1_RS07085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1419"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_108987606.1"
                     /transl_table=11
                     /translation="MFWVLFLLSAWAVAGLACLRLCLAAVRAAAVGPRAAAPEHTLTLY
                     EAAFLSGGPRRVADLTLVSMARQRRLLLAHTGWATVVDPCGRDDMERSVIGAIGPGGQS
                     RIAPVRAAAAAADAVRGLADRLVGAGLAVPDGGTGGVTAGVRRVRVAAVAVCALGLAAL
                     ALPLPATEPRPLIALWFALPLALTLGCLAITRMEIHPQARWASPAGQRLVGALVRQADG
                     TADDRTFLTSVAVRGVHAIGEPDLRAAFAHREKYAGDHWERRA"
     gene            7415..9022
                     /locus_tag="STPH1_RS07090"
                     /old_locus_tag="STPH1_1420"
     CDS             7415..9022
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (112-487). E-value:
                     5.9e-19. Score: 60.6. Matches aSDomain:
                     nrpspksdomains_STPH1_RS07090_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972878.1"
                     /locus_tag="STPH1_RS07090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1420"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_164382554.1"
                     /sec_met_domain="PF00561 (E-value: 8.1e-23, bitscore: 80.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYSAAGSLLLTTLAAVPAEATPVSAAGPGSAEASGTAVAA
                     RRAAAAGIEFGACPRAEELPDGIRCGTVSVPLDYARPDGKRIELTVSRVRATHKDPEGG
                     GRRVDGQGALLYNPGGPGASGLYFPLVGMVPEWKRIAAAYDLVGYAPRGVAPSAPLSCQ
                     DPGDFFKGPRPAPTRPSDAYKEQRAAEAEAYARDCAERGGDALRHYHSLNNARDLEVVR
                     AALGERRLTFMGASYGTYLGALYAELFPSHVRRMVFDAVVNPDPEQVWYRNNLEQSEAF
                     EDRWDDFKEWVAGHDAVYGLGDTARQVQDAYEEASARLAAEPAGGTVGPAQLQGAMLKA
                     GYYDDYWPHRAEALSAYLKGDPQPLIDQAGTRTEEGAVAAENSNAVYTAVECNDAPWPA
                     DFEVWDRDNTRLARRAPFETWDNVWTNLPCAYWRVPRQQPLDVRTAPGELPPTLVLAAE
                     RDAATPYEGALELRRRLSGSVLVTERDAGTHGIAGGPNDCVNGHLEAYLLDGRLPARDT
                     SCAPRPEPEPRQAAAPKA"
     aSDomain        7751..8875
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STPH1_RS07090_Abhydrolase_1.1"
                     /evalue="5.90E-19"
                     /label="STPH1_RS07090_Abhydrolase_1.1"
                     /locus_tag="STPH1_RS07090"
                     /protein_end="487"
                     /protein_start="112"
                     /score="60.6"
                     /tool="antismash"
                     /translation="ALLYNPGGPGASGLYFPLVGMVPEWKRIAAAYDLVGYAPRGVAPS
                     APLSCQDPGDFFKGPRPAPTRPSDAYKEQRAAEAEAYARDCAERGGDALRHYHSLNNAR
                     DLEVVRAALGERRLTFMGASYGTYLGALYAELFPSHVRRMVFDAVVNPDPEQVWYRNNL
                     EQSEAFEDRWDDFKEWVAGHDAVYGLGDTARQVQDAYEEASARLAAEPAGGTVGPAQLQ
                     GAMLKAGYYDDYWPHRAEALSAYLKGDPQPLIDQAGTRTEEGAVAAENSNAVYTAVECN
                     DAPWPADFEVWDRDNTRLARRAPFETWDNVWTNLPCAYWRVPRQQPLDVRTAPGELPPT
                     LVLAAERDAATPYEGALELRRRLSGSVLVTERDAG"
     gene            complement(9023..9754)
                     /gene="hemQ"
                     /locus_tag="STPH1_RS07095"
                     /old_locus_tag="STPH1_1421"
     CDS             complement(9023..9754)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="STPH1_RS07095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1421"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_007445291.1"
                     /transl_table=11
                     /translation="MSDDASTPAAERIPNKGKLAKDLNEVIRYTLWSVFKLKDGLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDLSGLRADADLMIWWHAETADQLQEAYNLFRRTK
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYISVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRGSEARRHVR
                     EEIPFFTGRRKDIGELVAGLA"
     gene            complement(9759..11243)
                     /gene="hemG"
                     /locus_tag="STPH1_RS07100"
                     /old_locus_tag="STPH1_1422"
     CDS             complement(9759..11243)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445289.1"
                     /locus_tag="STPH1_RS07100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1422"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_164382556.1"
                     /transl_table=11
                     /translation="MSASETPAGEFPPEQGPGHVVVVGAGIAGLAAAHRLLEAGARVTV
                     LEASDRVGGKLLPGEIAGVRVDLGAESMLARRPEAVGLARAAGLADRLQPPSTATASLW
                     TRGALRPMPKGHVMGVPGTAAALSGVLSAEGLARIGRDAELPRTEVGDDVAVGEYVAAR
                     LGREVVDRLVEPLLGGVYAGDAYRISLRSAVPQLFEAARTHTSLTEAVRALQGRTATSP
                     PSGPVFMGIEGGIGTLPPAVADLVRARGGEILTRAPVTELRRTASDGWRIVAGDRVLHA
                     GAVVVAVPAGPAAELLRAEAPAAAAELSAVEYASMALVTLAYRRSDAAALPEGSGFLVP
                     PVDGHTIKASTFASRKWGWIADEDPDLVVLRTSVGRYGDTEILGRDDEGLVAVSRHDLG
                     EATGLTAAPVATRVTRWQDGLPQYPVGHHARVARVREHVAKLPGLAVCGAAYDGVGIPA
                     SIASAYAAADQIRGDLRGVEELTAHPVRSLHGGAGE"
     misc_feature    complement(11279..11293)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: strong, score: 27.49"
                     /tool="antismash"
ORIGIN
        1 ggcgtcaccg ggtacaccag cgccaaactg accgtccagc acaccctggt ctacgacaag
       61 ctgcgccgca cccgcggccc cgagggcgcc cggctgtacg cccgctcgca gtccgaggcg
      121 gtcgagcgcg ccgccgggat cgtcgccgag ctgggcatcg actgcgagtg ggagacccgc
      181 gacgcgtaca cgtacgtccg cgacccgggc cgcgtcgacg aggtgcgggc cgaggcggcc
      241 gccgcgaagg aggcggggct gccggcgtcc ttcgtgacgg agaccgggct gccgttcccg
      301 gtggcgggcg ccgtgaaggt gaccgggcag gcgcagttcc accccctcaa gtacctgcgg
      361 gcgctcgccg cggacctgga ggcgcacggc gggcggatct tcgaggacac caccgtccag
      421 ggtctggacg agggcgagcc ctgccgggtg aggacggcgg cgggggcgac ggtcaccgcc
      481 cgggacgtcg tggtcgccac ccactacccg atcttcgacc gtgccctgct cttcgcccgc
      541 ctctccccgc gccgcgagct ggtcgtcgcc gggaccgtcg aggcggaccg cgacgtggac
      601 ggcatgtaca tcacgccgga ggagaatacc cgctcggtgc gcacggcgcc cctggacgac
      661 gcgggccgcc gcctgctcct cctcaccggg gagcacttca cgccgggcac gggcgacacc
      721 cgcgagcgct tcgcgaccct ggccgcgtgg gcggacgagc acttccccgg cgtcgagcgc
      781 acccatgcct gggccacgca ggacatcgac cccaccgaca ccgtggcgat ggcgggcccg
      841 ctgcacccgg gcgcgcacca cacgtacgtc gccaccggct tcggcggctg ggggctgagc
      901 ggcggcatca tggcgggccg gctgctgacc gcgcagatca ccggcgagga gtgcgcgtgg
      961 accgggctgt acgacccgcg ccggctgcgc acggcggtgc gcgaggcgcc ggcgctgctc
     1021 aagacgcagg ccgaggtggc ccggcacttc gtcggcgacc ggctgcggcc caccccgccg
     1081 gtggaggcgc tgccgccggg cgagggcgcg gtggtccggg cgggcggtga ccggctggcg
     1141 gtctaccggg acgaggaggg cagcctgcac gccgtctcgg cgcgctgcac ccacctgggc
     1201 tgcctggtcg acttcaacgc ggccgagcgt gcctgggagt gcccctgcca cggctcccgc
     1261 ttcgacacgg acggcaaggt gatcgagggt ccggcgacga agccgctgga gcagcgggac
     1321 atctgacccc gggggtctga gccgggtggg tgaccgggtc ggcacgtcga cccacatagt
     1381 catacgaacc gaacatctca cttctacgtt cgtgcggtga tcagtctcgt acgacgtacc
     1441 accgcactct gcgtcctcgg cgccgccctc gccgcctgcg gcacccccga cgccggccag
     1501 accccccgtc ccgcaccctc ggcccccgcc ccctcctcct ccccgtctcc ctcccggacg
     1561 cccaccctcg ccccgggccc cgcgggcctc acccccgtct tcgagcacgg gccccgcacc
     1621 gacggcgcga aggacggcag gaagaacggc aaaacggtcg ccctcacctt cgacgccgac
     1681 atgaccgccg accaggggcc gcgggcggcg tcgggcgaac ggttcgacaa cccgcagctg
     1741 atcgcgacgc tgcggaggct gaaggtgccg gccacggtct tcatgaccgg ccgctgggcc
     1801 gaggagtacc cggaccaggc ccgctccctc ggccgggacc cggggttcga gatcgccaac
     1861 cactcctaca gccactacgc ctacaccgac gactgctacg gcctgccgac ggtctccaag
     1921 gacgacatgc gcaccgacct ggagcgcgcg tacgccgcct tcgagaaggc cggggtgccg
     1981 aacccgatgc cgtacttccg cttccccggc ggctgttacg acaaggcggc cctcaaggag
     2041 ctgtcggcga cgggtgtcac cgccgtgcag tgggacgtgg tgagcgggga cgcgttcgcg
     2101 acggacgccg acgcggtgac ccggcaggtc ctcgacgggg tgcggccggg gtcggtcgtc
     2161 gtcatgcact gcacgcgcag cgccgccccg gtgaccgagg aggtggtgcg ggcggtcgtg
     2221 cccgagctgc gccgccgggg ctaccgcttc gtcaaggtct ccgaactgat cggcgccgcg
     2281 aacgggcgcg gctgacgtcc cgcgccgacg tcctacgctg gagacatgac cagcgaccac
     2341 gaccaaggcc cggacggctc cggctccggc tgcgagcccg gcggctactc cctgatcgac
     2401 tcggcgcccc ggccgcccag ggccgacggc ccgccgtacg cggactgcgt cgtgtgccgg
     2461 gagccgacgg agtacccgga gtcgtacaag gggatcacgc tctgcccggt ctgcgagtgg
     2521 caggaggccc agcggaccgc ctgttcaggc tgacgtcctg cgcgagctga gcgcacaggc
     2581 cgcggcggta gcggccgcgg cgacgaccgc cgccccggcc agcggcagca gcggcagcgg
     2641 gaccgcgccc gacttcgacc cggtgaccag gccgctgacc gccgcccgtg ccggggaccc
     2701 cgacgccacc agcgccagca gggccgccag cagcagggcg ggcaccgcgc ggccgggcga
     2761 gcgcagcacc ggccaggcgg tgagcgcgcc gacggcggtg ccgagcaggg cgcacaccag
     2821 cgcggcgagc agtccggcac cgccggcggg gagcagcggt acgcgtgtgc cgtgaccggt
     2881 gctgaccggg tcgctgatca gcgtcacgac gacggtcgcc accgcgccca ggacggtcgc
     2941 ggcggccagc gccacgagca cggaggccag atgggcccgc gcgggaccgc gggccgcgga
     3001 cacgcacgcc cgcgcggcgg acggctcacc ggtgacgcag atccgcacca gccaggccgc
     3061 gaccggcagc agcacggccg ccgcccagcc cagcgagtcg agcaccggct gccctccctg
     3121 tactccgacg ccgagcgcgg cggcatacag gacgacgggc ggcagccagc gctgggagcg
     3181 cagcaggagg gccgcctggt agcgcaggag agcggtcatc gggggtcctc cggggcgtgc
     3241 acgctcacca cgtgccaggg cggacgggcg gtcaggagcg tgcgcagcag gacgtcggag
     3301 tgcgaggcgg ggacaccgat cgtgtgggac ccggggccgg tctcctccac cgaggcggcc
     3361 gtccggtacg cgtcggcggg cagggcggcg cccggcggcc cctgcacccg gacgacgacc
     3421 cgcggtccgg cggccgccgg ggcgggaccg gtacggcggt gcagcgtgcc gtcgcgcacg
     3481 gtgtacacgg cgtcgggcgc cccggccagg cggcccggat cgtggtcgac gaagaccacg
     3541 gcaccgcccc ccgccacccg ttcggcgacc gcccgttcca gctccccccg cgcgcccgcg
     3601 tcgagcccgg tccaggcctc gtcgaggacc agcagctccg gctcggcgac cagcgcctgc
     3661 gccaccgcca ccttctggct gccgcccttg gacagccggg agagccgggt gccggcgtac
     3721 ccggcggccc ccagccgctc cagccactgc cccgccgcac gggccgccgc acggcgggac
     3781 agcccgtgga cggtgccgag gtgggtgagg tacccggcgg cggtgaaggg cagggcgggc
     3841 gggaagcgtt cggggacgta ggcggtacgg gggcggccgg tgaccctgcc ctcggtgggg
     3901 gcgtcgatcc cggcgagcag ccgcagcagc gtggacttgc cggtgccgtt ggtgccctcg
     3961 acgcggacga gggtgccgga cggcacgccg aggtcgacgc cgcgcagcac ccaggggccg
     4021 cggatgccgt accggcggcc cacgccgtcc agccgtagct cgcgttgcat ggggcccatc
     4081 ctctcgcggg cgggaggccc ggcggccccg ggcgggcgac ttggcagact gtagggcgtg
     4141 agcaacgacc cgacgacgcc cgtccccgcc tccgtcccag ccgcggcccc ggcctcggcg
     4201 ccgacccccg acagtccctt ccgcgccgag cccggcgacc gcgacctcgc gccgcagttc
     4261 gtgctcccgc tcgtcgtgcg catcgagcgg gcggcgccgc caccccgtac cgacgcgctg
     4321 gagaccgcgg cgcgcgcggt gctggtgatg ctcggcgacg agcggtcgac cggtgacggc
     4381 gcgtgggcgc gggcgatgcg ggactggcag gacgcgcgca tccgcaaggt cgtacgccgg
     4441 gcccggggcg cggagtggcg gcgggccgag gcactgcccg gcatcacggt caccggcaag
     4501 agcgccgagg tgcgggtctt cccgccggtc ccgctggacg gctggcccaa ggacctggcc
     4561 cgcctccagg tctccggcac cgacctcgac gacctcgagc cgccggccgc cgccgacccc
     4621 gccgcgccgg tgctctggat gaacccggac ctggacatgt cggcgggcaa ggcgatggcc
     4681 caggccggcc acggggcgca gctcgcctgg tgggcgctgt ccgacggcga gcggtcggcg
     4741 tggcgcgagg cgggcttccc gctgtcggta cgcaccgcgg acccggcccg ctggggcggc
     4801 ctcaccaccg gcggcctgcc ggtggtgcgc gacgccggct tcacggagat cgcgccgggc
     4861 tcgtgcacgg tggtcgcgga ccatccggcg ctgcggcggt aggaacctca gatgttgctc
     4921 tgaacaagcc ccgcccgtgg ttcggcccgg gccgggcggg cgatatcccc ggcatgtgag
     4981 cggcgaaggg ggacgcgatc atggagcgaa cggcgcgact gggcaccgga atcggatggc
     5041 ggccggagat cgcggacgcc gtcgagcgga tgcccggcat cgactgggtc gaggcggtgt
     5101 ccgagaacct gtgtcccggg catctgcccg actcgctgct gcggctgcgc gagcgcgggg
     5161 tcaccgtcgt accgcacggg gtctccctcg ggctcggcgg cgcggagcgg ccggacgcgg
     5221 ggcggctggc ggcgctggcc gagcgggcgg aggtcctggg ggcgccgctg gtcaccgagc
     5281 acatcgcgtt cgtccgggcg ggcggcgcgc tgaccgcctc accgccgctg gaggcggggc
     5341 acctgctgcc cgtgccgcgc acccgtgacg ccctcgacgt gctgtgcgag aacgtgcgca
     5401 tcgcgcagga cgcgctgccc gtgccgctcg ccgtggagaa catcgcggcg ctcgttgcct
     5461 ggccggacga ggagctgacg gaggggcagt tcctgtacga gctggccgac cggacggggg
     5521 tgcggctgct gatcgacgtg gccaacctgc acaccaacca cgtcaacctc ggcgcggacc
     5581 cggccgaggc gctcgccgaa ctccccctgg aggccctcgc ctacgtccat gtcgcgggcg
     5641 gcttcgagcg ggacggcgtc tggcacgaca gccacgccca ccccgtcgcg cagccggtgc
     5701 tcgacatcct gaccgacctc gcctcgcgtg tcgcaccgcc gggcgtcctg ctggagcggg
     5761 acgagaactt ccccgacggc gacgggctga gcggtgaggt ggaggcgatc cgcaccgccg
     5821 tcgcgaaggg gcgggccggg agccccgggg ccgtgacggg cggggcggcg ccgcgcacct
     5881 cgggcgccgg gtccgggtcc cggtccgggt gccggtccgg gtccgggtcg ggttcgggtt
     5941 ccggttccgg ttccgggtcc accggcgacg ccgtacggca gcggctcggg ctcgcgcagg
     6001 ccgcgctgct gtcggcgctg gtcgcgggga cgccggtgcc cgaggggttc gaccgggtgc
     6061 ggctgggggt gcaggcccgg gcgctcgccg ggaagcgggc cgacgtggtc gggaaggtcg
     6121 caccggagct gccggtgatc ctgggcgcgg ggtaccggcc ggccttcctc gcctacgcgc
     6181 aggggtcccc gctgaccggc ggctaccggc gggacgccct cgacttcgcc gcgcacgtgc
     6241 tgcggacggc gccgggggac gaggacccgg ccgtgcggag ggcgttgcgg gagtggtggc
     6301 tggaccggtc ggggccggtg ccgcgctcca ggcgtcccgg ggcccggctg gcccgtgcga
     6361 ccaggcgggt tctgctgcgc cgctgagccg ggggacggcc tcgggggaca tcaccaaacg
     6421 gaacgtcaca tacccacaac accgtgtccc ggaatgtgaa cagggcgtgg cgccggcgaa
     6481 agccttggca tagatatacg gcatgttctg ggtccttttc ctcctgtcgg cctgggccgt
     6541 cgccggcctc gcctgtctgc ggctgtgcct ggccgccgta cgcgcggccg ccgtcggccc
     6601 gcgcgccgcg gctcccgagc acacgctgac cctgtacgag gcggcgttcc tgtccggcgg
     6661 cccgcggcgg gtggccgacc tgaccctcgt ctccatggca cgccagcggc ggctgctgct
     6721 cgcgcacacc ggctgggcga cggtcgtgga cccgtgcggc cgggacgaca tggagcggtc
     6781 ggtcataggg gcgataggcc ccgggggaca gtcccggata gcgccggtac gggccgccgc
     6841 ggccgccgcc gacgcggtac ggggcctggc cgaccggctg gtcggcgcgg gcctcgccgt
     6901 acccgacggc ggcaccggcg gtgtcacggc cggggtgcgg cgggtgcggg tcgccgcggt
     6961 ggccgtgtgc gcgctcggcc tggccgctct ggcgctgccg ctgcccgcca ccgagccgcg
     7021 cccgctcatc gccctctggt tcgcgctgcc gctcgccctg acgctcgggt gcctcgccat
     7081 cacgcgcatg gagatccacc cgcaggcgcg ctgggcctcc ccggccgggc aacggctggt
     7141 cggcgccctc gtccggcagg cggacggcac ggcggacgac cggaccttcc tcacctcggt
     7201 cgccgtgcgg ggcgtgcacg cgatcggcga gccggacctg cgggcggcct tcgcccatcg
     7261 cgagaagtac gccggggacc actgggagcg ccgggcctga cgcgccgggg gcgtgcgggg
     7321 gcactcggcg gcgacacccg cgggcggtgc ttgccccgcc gggccgccgc accaaacatc
     7381 ccttgtgtca caccgtgcac cgaagggata cgcgatgcga gctgccgccc tctactcggc
     7441 cgccgggtcc ttgctgctga ccaccctcgc cgccgtgccc gcggaggcca ccccggtgtc
     7501 cgcggccggg cccgggtccg ccgaggcgag cgggaccgcc gtagccgccc ggcgcgcggc
     7561 ggcggccggg atcgagttcg gggcgtgccc gagggcggag gagctgccgg acggcatccg
     7621 ctgcggcacg gtgtccgtgc ccctggacta cgcccgcccc gacgggaagc ggatcgagct
     7681 gaccgtgagc cgggtgcggg ccacccacaa ggaccccgag ggcggcggac gccgggtgga
     7741 cggacagggg gccctcctct acaacccggg cggccccggc gcctccgggc tgtacttccc
     7801 gctggtcggc atggtgcccg agtggaagcg gatcgccgcc gcctacgacc tcgtcggcta
     7861 cgccccgcgc ggcgtcgcgc cctccgcgcc gctgtcctgc caggaccccg gcgacttctt
     7921 caaggggccg cgccccgccc cgacgcggcc ttcggacgcg tacaaggagc agcgggccgc
     7981 cgaggccgag gcgtacgcgc gcgactgcgc ggaacggggc ggggacgcgc tgcggcacta
     8041 ccactcgctc aacaacgccc gtgacctgga ggtcgtccgc gccgcgctgg gcgagcggcg
     8101 gctcaccttc atgggcgcgt cgtacggcac ctacctcggc gcgctgtacg ccgagctgtt
     8161 cccctcgcac gtacggcgga tggtgttcga cgcggtggtg aacccggacc cggagcaggt
     8221 ctggtaccgg aacaacctgg agcagtcgga ggcgttcgag gaccgctggg acgacttcaa
     8281 ggagtgggtc gccgggcacg acgccgtgta cgggctcggg gacacggcgc ggcaggtgca
     8341 ggacgcctac gaggaggcga gcgcacggct ggcggcggag cccgcgggcg ggacggtggg
     8401 gcccgcgcag ttgcagggcg cgatgctgaa ggccgggtac tacgacgact actggccgca
     8461 ccgtgccgag gcgctgtcgg cctatctgaa gggcgacccg cagccgctga tcgaccaggc
     8521 ggggacgcgg accgaggagg gcgccgtcgc ggcggagaac tccaacgccg tgtacacggc
     8581 cgtcgagtgc aacgacgcgc cctggccggc cgacttcgag gtctgggacc gggacaacac
     8641 ccggctggcc cgccgggcgc cgttcgagac ctgggacaac gtgtggacga acctgccgtg
     8701 cgcctactgg cgggtgcccc ggcagcagcc cctcgacgtg cgcaccgcgc cgggtgagct
     8761 gccgccgacg ctggtcctgg ccgcggaacg ggacgcggcg acgccgtacg agggcgcgct
     8821 ggagctgcgg cggcggctgt cgggctcggt gctggtgacc gagcgggacg cggggacgca
     8881 cggcatcgcg ggcgggccga acgactgcgt caacggccac ctggaggcgt acctcctcga
     8941 cggccgcctc ccggcgcggg acacgtcgtg cgccccgcgc ccggaaccgg agccgaggca
     9001 ggccgcggcg ccgaaggcct gactaggcga gcccggcgac cagctcgccg atgtccttcc
     9061 gacgacccgt gaagaacggg atctcctcgc ggacgtggcg acgcgcctcg gagccgcgca
     9121 ggtggcgcat gaggtcgacg atgcggtgca gctcgtcggc ctcgaaggcg aggatccact
     9181 cgtagtcgcc cagcgagaag gaggcgaccg tgttggcacg cacgtccggg tagccgcggg
     9241 ccatcttgcc gtggtcggcg agcatgcggc ggcggtcctc gtcgggcagc aggtaccagt
     9301 cgtacgagcg cacgaacggg tagacgctga tgtagttgcg cggcgtctcg tcggccagga
     9361 aggccgggat gtgcgagcgg ttgaactcgg cggggcggtg cagcgccatg ttcgaccaga
     9421 ccggctccag cgcacggccg agcttcgtgc ggcggaagag gttgtacgcc tcctggagct
     9481 ggtcggcggt ctcggcgtgc caccagatca tcagatcggc gtccgcgcgc agaccggaca
     9541 ggtcgtaggt gccgcggatc gtcacgtcct tcgcggcgag ctggtcgaac agctcctgga
     9601 cctcgtcggc gtacccggcg cggtcctcgg ggaggccgtc cttgagcttg aacacggacc
     9661 agagggtgta ccggatgacc tcgttgaggt ccttggccag cttgcccttg ttcgggatcc
     9721 gctcggcggc gggggtggag gcgtcgtcac tcatggccct attctcccgc tccgccgtgc
     9781 agactccgca ccgggtgggc cgtgagctcc tccacaccgc gcaggtcgcc ccggatctgg
     9841 tcggcggcgg cgtacgcgct cgcgatgctc gccgggatgc cgacgccgtc gtacgccgcg
     9901 ccgcacaccg cgaggcccgg gagtttcgcg acgtgttcgc ggacgcgggc cacgcgcgcg
     9961 tggtggccga cggggtactg cggcaggccg tcttgccagc gggtgacgcg ggtggcgacc
    10021 ggcgcggcgg tcaggccggt ggcctcgccc aggtcgtgcc gggagacggc gacgaggcct
    10081 tcgtcgtcgc ggccgaggat ctccgtgtcg ccgtaccggc ccaccgaggt gcgcaggacg
    10141 accaggtccg ggtcctcgtc ggcgatccag ccccacttgc gggaggcgaa ggtggacgcc
    10201 ttgatggtgt gcccgtcgac cggcggtacg aggaagccgc tgccctcggg gagcgcggcg
    10261 gcgtccgagc ggcggtaggc gagggtgacc agggccatcg aggcgtactc caccgcggac
    10321 agctcggccg cggcggcggg cgcctcggcg cgcagcagct cggcggcggg accggccggg
    10381 acggcgacga cgaccgcgcc ggcgtgcagc acccggtcgc ccgcgacgat ccgccagccg
    10441 tcggacgcgg tgcggcgcag ctcggtgacg ggcgcccgcg tgaggatctc gccgccgcgc
    10501 gcgcggacca agtcggcgac ggcgggcggc agggtgccga tgccgccctc gatgcccatg
    10561 aacaccgggc cgctcggcgg tgaggtggcc gtgcggccct ggagggcgcg gaccgcctcg
    10621 gtgagcgagg tgtgggtgcg ggcggcctcg aagagctggg gcacggccga gcggagcgag
    10681 atgcggtagg cgtcgcccgc gtagacgccg cccagcagcg gctcgacgag gcggtcgacg
    10741 acctcgcggc ccaggcgcgc ggcgacgtac tcccccacgg ccacgtcgtc gccgacctcg
    10801 gtgcggggca gttcggcgtc gcgcccgatg cgggcgaggc cctccgcgga gagcacgccg
    10861 gacagggcgg cggcggtgcc gggaacgccc atgacgtggc ccttgggcat ggggcgcagg
    10921 gcgccgcggg tccacaggga ggcggtcgcg gtggacggcg gctggaggcg gtcggcgagt
    10981 ccggccgcgc gggccagacc gacggcctcg gggcggcggg ccagcatcga ctcggccccg
    11041 aggtcgacgc gtacgccggc gatctcgccg ggcagcagct tgccgccgac gcggtcggac
    11101 gcctccagga cggtcacccg ggcgccggcc tccaggaggc ggtgggcggc ggccagtccg
    11161 gcgatgccgg ctcctacgac gactacgtgt ccgggcccct gttccggggg gaactcaccc
    11221 gccggggtct cacttgcgct catgacccca gcgtctcaga cgccaccgac accgccccgg
    11281 ggccggacgg ccccggcccc gggcccacag gtcccgcgcg acccgttcgc tcgtccggcg
    11341 ccggtcccgc cgcgagtccg gccgcgagtc cggaccgtga tctctt
//
