LOCUS       NZ_WHZR01000001        12939 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces sp. OM5714 A912_AS4_SC1_R2, whole genome shotgun
            sequence.
ACCESSION   NZ_WHZR01000001
VERSION     NZ_WHZR01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. OM5714
  ORGANISM  Streptomyces sp. OM5714
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7915160)
  AUTHORS   Samborskyy,M.
  TITLE     Whole Genome Sequence of the phthoxazolin producer Streptomyces sp.
            OM5714
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7915160)
  AUTHORS   Samborskyy,M., Dickens,S. and Scott,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2019) Biochemistry, DNA Sequencing Facility,
            University of Cambridge, 80 Tennis Court Road, Cambridge,
            Cambridgeshire CB2 1GA, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2017
            Assembly Method                   :: Newbler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 102.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010993835.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:48:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,820
            CDSs (total)                      :: 7,730
            Genes (coding)                    :: 7,557
            CDSs (with protein)               :: 7,557
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 7, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 7, 5 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 58 of 173
            Pseudo Genes (incomplete)         :: 139 of 173
            Pseudo Genes (internal stop)      :: 18 of 173
            Pseudo Genes (multiple problems)  :: 36 of 173
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:03:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1375353
            Orig. end                         :: 1388292
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHZR01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12939
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="8"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12939
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12939
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7939](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7939)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    465..480
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.91"
                     /tool="antismash"
     misc_feature    527..537
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    complement(694..706)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.71"
                     /tool="antismash"
     gene            694..3012
                     /locus_tag="STPH1_RS05805"
                     /old_locus_tag="STPH1_1161"
     CDS             694..3012
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030528.1"
                     /locus_tag="STPH1_RS05805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1161"
                     /product="M9 family metallopeptidase"
                     /protein_id="WP_164382205.1"
                     /transl_table=11
                     /translation="MRKSLLRRSLGAAVPLALAVAMVAGLPAQPAGAVSGQPVSAAPRA
                     ADADAGDPHAGPPPVAQSVTAGTDHVGEGRLKASALPPLAASKDALRESYGAPEEQRKP
                     RGADGTKTAAAACDVSAFTTRTGSALVQQIKSSTTDCVNTLFNLTGNDAYQAFREAQMT
                     SVAYALRDGSASYPGNAGTGMPQLVLYLRAGYYVHYYNAGTVGGYGSGLRTAIRAGLDA
                     FFAAPHSRDVSDANGETLAEAVTLIDSAEENARYIHVVERLLADYDSSWNSSWWMLNAV
                     NNVYTVTFRGHQVPAFVTAVESDPGLLDALYGFASGHLSLLGTDQSYLTSNAGRELGRF
                     LQHSTLRSKVRPLATGLLNSSSITGATAPLWVGVAEMTAFYDAADCSSYGTCDLPAQLA
                     KAVLPVTYACSSSITIRAQQMTSGELAASCGSLRSQDAYFHDVVRDNGPVAGDRNGTIE
                     VVVFDSSTDYQTYAGAMYGIDTNNGGMYLEGDPSAAGNQPRFIAYEAEWLRPDFQIWNL
                     NHEYTHYLDGRFDMYGDFNANITTPTIWWVEGFAEYVSYSYRGVPYTEAMTEAGRGTYA
                     LSTLFDTTYSHDTTRVYRWGYLATRYMLERHRADMDTVLGHYRSGDWNAARTYLTATIG
                     TRYDNDWYTWLAGCAAGDCGTGTGTGPGAECTGTDTRELGRDCRRSDQSATKGNYAYLY
                     LHVPAGTTRLTITTSGGTGDADLYYGDSGWPGTGGYTRRDTGTGNSHTLTVDNPAAGTH
                     YISLYAVDGFNGVTVGTDY"
     gene            complement(3104..4105)
                     /locus_tag="STPH1_RS05810"
                     /old_locus_tag="STPH1_1162"
     CDS             complement(3104..4105)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="STPH1_RS05810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1162"
                     /product="proline racemase family protein"
                     /protein_id="WP_164382207.1"
                     /transl_table=11
                     /translation="MRSTVCYHAVDSHTEGMPTRVITGGVGALPGATMFEKRQRFVAER
                     DHLRTLLMCEPRGHASMSGAILQPPTRPDADYGVLFIEVSGCLPMCGHGTIGVATVLVE
                     TGMVEVTEPETRVRLDTPAGLVTARVRVRDGRAESVTLENVASYCHALDQVVDVPGYGQ
                     VRYDLAYGGNFYAFVRTDDLGIPFERAHKQRLLDAGLAVMDAVNKQNPVSHPENPEIDV
                     CHHVYLEAPGSTAEHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGLLPAGRDFVNES
                     FIGSRFVGRILGETTVGDRPAVLPSVTGRAWITGTAQYLLDPTDPYPAGFTL"
     gene            complement(4108..5004)
                     /locus_tag="STPH1_RS05815"
                     /old_locus_tag="STPH1_1163"
     CDS             complement(4108..5004)
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030799.1"
                     /locus_tag="STPH1_RS05815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1163"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_164382209.1"
                     /transl_table=11
                     /translation="MSETRKPWHGVIVATSLPFHDDLSVDFGAYGESVAHLAEQGMHGV
                     APNGSLGEYQTLTYEERDRVVETAVAHAPAGFTVMPGVGAYGGEEARRHAQFAKDAGCQ
                     AVMCLPPNAYRADDRAVLEHFEAVASVGLPVTAYNNPIDTKVDLRPDLLAKLHAEGFVV
                     GVKEFSGDVRRCYEISELAPGLDLMIGTDDTVLEVALAGAKGWVAGYPQVFPRACLALY
                     EASVRGDLETALPLYRRLHPVLRWDSKTEFVQAIKLGQELTGRRGGGCRPPRQPLAPET
                     EAVVRAATQALIDAGVN"
     gene            complement(5001..6527)
                     /locus_tag="STPH1_RS05820"
                     /old_locus_tag="STPH1_1164"
     CDS             complement(5001..6527)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030798.1"
                     /locus_tag="STPH1_RS05820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1164"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_164382210.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 7.5e-18, bitscore:
                     63.4, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 2.6e-11, bitscore:
                     42.4, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREPADLVVVGAGPAGLAAAVTALTGGLRVVLVDSGTTVGGQYWR
                     HPPEHARAAVPTGDLHHGLRTYRALCRALATHRAAGRLDLLLGHHAWSAVREGDGFAVH
                     AVDRRDAPRETPVVLRAPRLLVATGAYDRQLPFPGWDLPGVLTAGGLQALLKGGGVAAG
                     SRVALGGTGPFLLPVAAGLAARGVEVVAVCEAAHPRAWLRHPVPLLRNPGKWAEGAGYA
                     ATLARHRVPVRPRTAIVAAEGGARVSSVRIASLAPDGSPRPGTERRVEVDAVGVGWGFT
                     PQLDLLVPLGCALTESADGNAVAAVDADQRTTVPGLYTAGETCGVGGAALALNEGRLAA
                     RSVLADSGTSPAPANRPPTALRSAVVRQRAFARAMALAHPVPPAWPAWLTEETVVCRCE
                     EVTAGAVRAARADGAHDHRQVKQLTRAGMGWCQGRMCGPAVHCLTAPGRAYEPAERLVA
                     TPVTLGALADLDDGPRAPQAPQAPQTPQAPTARQAPTAPTDNGSTDKECS"
     gene            complement(6520..6774)
                     /locus_tag="STPH1_RS05825"
                     /old_locus_tag="STPH1_1165"
     CDS             complement(6520..6774)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972635.1"
                     /locus_tag="STPH1_RS05825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1165"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_153177194.1"
                     /sec_met_domain="Fer2_4 (E-value: 6.8e-25, bitscore: 85.6,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEITVDGEPLPFVEGQTVAAALVAAGRVAWRSTRVGHRPRGVFC
                     GIGVCFDCLLTIDGTGGQRACLVPARPGMTVTTREDDDA"
     gene            complement(6767..7939)
                     /locus_tag="STPH1_RS05830"
                     /old_locus_tag="STPH1_1166"
     CDS             complement(6767..7939)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445543.1"
                     /locus_tag="STPH1_RS05830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STPH1_1166"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_164382211.1"
                     /sec_met_domain="DAO (E-value: 5.1e-68, bitscore: 228.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLKVVVVGAGVVGAACAYHAASAGLDVTVVDRGPVGAGTTSRGE
                     GNVLLSDKEPGPELALAHLSRALWDEAGRELGPDTLELEPKGGLVVASTAESLTALGEF
                     AARQEAAGVRVEPVERVRDLEPHLAPGIPGGVHYPQDAQVQPVLAAAALLRAAVRHGAR
                     FRTGEVSGAVTTRDGGRITGVRTAAGDVLPADAVVNAAGTWGGEVGRRLGAPVEVLPRR
                     GFVLVTEPLPPMVRHKVYSADYVANVASSDAGLETSCVVEGTRGGTILVGASRERVGFD
                     TTMNTAVVARLAAQACRLFPFLRGVHLMRAYRGFRPYCPDHLPVVGPDPRVPGVLHACG
                     HEGAGIGLAPATGALVTAHLLGRPWRGADPAAHTGLLPDRFLDPEVRPSE"
     misc_feature    7968..7984
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.16"
                     /tool="antismash"
     misc_feature    7972..7982
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     gene            complement(8081..10369)
                     /locus_tag="STPH1_RS39360"
     CDS             complement(8081..10369)
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999670.1"
                     /locus_tag="STPH1_RS39360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SWIM zinc finger family protein"
                     /protein_id="WP_240469177.1"
                     /transl_table=11
                     /translation="MAREALRAARRQAKAEAASGRAASARRTPPPAARRRRPGGADTAD
                     ARARAVRDFAADAFRLPPETDPAGDATLRGETPAAGSPAGGPSGADWAAPYPERATSPD
                     AGPVTEPTVAPNAGDTASAEGGATGSGQPGHAGRAAHTEGGAPGPGGPGHAVRTTSPES
                     LPPGPGMPTPEHALHAVSPEGPPPGPGEPGHAGRAAHTEGGAPGPGGPGHAAPTTSPES
                     LPLVPEWSGHAARTTSLESLPPGPEWPGHVSRAASLEGASPVPERSGHPPRSASSEGRV
                     SGPEWPGHASRTASSEGPVSAHERSGRPRYPRDPAHPSAAGGREPHSAPVAQARAPRSM
                     AAPGRDGDLRRTFPALPPRRAADEGLAGTWWGNAWVRALEEGALDVARLERGRGYAEGG
                     HVDAITVTPGLVLAYVQGSRARPYRVQVRLRTLGDADWDRFLDAAVERPGHIAALLDKE
                     LPHSLADLADRGVPLLPGPGDLTPQCSCPDSGHPCKHAAALCYQTARLLDADPFVLLLL
                     RGRGERALLDALSRRNAARAARAAQDRGPAPLPGLRAGEVLARRALPPLPAPLPAPPHP
                     EQPPAYPAAPGGPDPFALDQLATDAAARAHALLTTGRDPVGTLTLWQDAVRLAAARPGS
                     GLTAGTRALYASLASAAGRDTAELARAVAAWRQGGPQGLDVLEEPWDPPAGRFDRARPL
                     LLAADLPAFRPWRNHLTHPRGHVQLRLGRSGLWYAYESEPGREDWWPRGTPDLDPVGAL
                     TGLGTRVDL"
     misc_feature    10483..10497
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.01"
                     /tool="antismash"
     misc_feature    10971..10985
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
     misc_feature    12077..12092
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.56"
                     /tool="antismash"
     misc_feature    complement(12284..12297)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.58"
                     /tool="antismash"
ORIGIN
        1 ttcaccgaga tccgcacgct gatcgaggtt cccaccgtgg gccgggtcgc cgcgacggcg
       61 accgcggagc agttggaggc cctgcgtccg ctggcgcggc agatcgtcac cgcagcacgg
      121 gagcacgacg tcctcaagta cctcgaggcc gaccaccggt tccacctcga actgctggcc
      181 ctggccggca accaccatct cgtggaggtg gtggccgacc tgcgcaagcg ttcacgcctg
      241 ttcgggctgg gcagcctgaa cgagacgggc cggctggtcg cttccgccga ggagcacgtc
      301 gaactgctgg acctgatggc ggccggccgc agccgcgagg cggaggagtg catgcgccgc
      361 cacctcaccc acgtccgcac gctgtgggcg gacggcaggg cggacgccga ggcgggcatc
      421 gaggcctgac ggagcgcgcc gtcgtcaagt cccttccgct gtggttcagt tcgctttcac
      481 aggcggggaa attcgtcacc gtggattccg tcggaccact tcccggcgcc atgtcacctg
      541 tgcaatggta catggcacag gtcgtggtca tgtcaatctg taggcatcgg ccgtgccccc
      601 gtccgttcga cggcgcggcc ctccccgatc cacggccttc tcatcctcca agtggccccg
      661 cgtcgcgggg tccccaccct catgggaggc gtagtgagaa agtcacttct ccgtcggagt
      721 ctgggcgcgg cggtgccgct cgccctggcc gtcgccatgg tcgccgggct gccggcgcag
      781 ccggccggcg ctgtgagcgg gcagccggtg tccgccgcac cacgggccgc cgacgcggac
      841 gcgggtgacc cgcacgccgg tcccccgccc gtggcccagt ccgtcaccgc cggcaccgac
      901 cacgtgggcg agggacggct gaaggcgtcg gcgcttccgc cgctggcggc gagcaaggac
      961 gcgctcaggg agtcctacgg ggcacccgag gaacagcgga aaccgcgggg cgcggacggg
     1021 acgaagaccg cggcggccgc ctgcgacgtc tccgcgttca ccacccgcac cggcagcgcg
     1081 ctggtccagc agatcaagtc gtcgacgacc gactgcgtca acaccctgtt caacctgacc
     1141 gggaacgacg cctaccaggc cttccgcgag gcgcagatga cctccgtcgc ctacgccctg
     1201 cgcgacggtt cggcgtcgta cccgggcaac gccggcaccg gtatgccaca gctggtgctc
     1261 tacctccgcg ccggctacta cgtccactac tacaacgccg gtacggtggg cggctacggc
     1321 agcggcctgc ggaccgcgat acgcgcgggg ctcgacgcgt tcttcgccgc cccgcactcc
     1381 cgcgacgtca gcgacgccaa cggcgagacg ctcgccgagg ccgtcaccct catcgacagt
     1441 gccgaggaga acgcccgcta catccacgtc gtcgaacggc tgctggcgga ctacgactcc
     1501 tcctggaact cctcctggtg gatgctcaac gcggtcaaca acgtctacac ggtgaccttc
     1561 cgcggccacc aggtgcccgc gttcgtgacc gccgtcgagt cggaccccgg cctgctcgac
     1621 gccctgtacg gcttcgcctc cgggcacctc tcgctcctgg gcacggacca gtcgtacctc
     1681 acgtccaacg cgggacgtga actcggcagg ttcctccagc actccaccct gcggagcaag
     1741 gtacggcccc tggcgaccgg cttgctgaac tcgagctcga tcaccggcgc caccgccccg
     1801 ctctgggtcg gcgtcgccga gatgaccgcc ttctacgacg cggccgactg ctcctcctac
     1861 ggcacctgcg acctgccggc gcagctggcg aaggcggtgc tgccggtgac gtacgcgtgc
     1921 agctccagca tcaccatcag ggcccagcag atgacctcgg gcgagctggc ggccagttgc
     1981 ggcagccttc gcagccagga tgcctacttc cacgacgtcg tccgtgacaa cggtcctgtc
     2041 gcgggcgacc gcaacggcac catcgaggtc gtggtcttcg actccagcac cgactaccag
     2101 acctacgccg gtgccatgta cgggatcgac accaacaacg gcggcatgta cctggagggc
     2161 gacccgtcgg cggccggcaa ccagccgcgc ttcatcgcct acgaggccga gtggctgcgc
     2221 ccggacttcc agatctggaa cctcaaccac gagtacaccc actacctcga cggccgcttc
     2281 gacatgtacg gcgacttcaa cgccaacatc accacaccga ccatctggtg ggtcgaaggc
     2341 ttcgcggagt acgtctccta ctcctaccgc ggtgtcccct acacggaggc gatgaccgag
     2401 gcgggccgcg gcacgtacgc cctgagcacc ctcttcgaca ccacgtacag ccacgacacc
     2461 acccgcgtct accgctgggg ctacctcgcc acccggtaca tgctcgaaag gcaccgcgcc
     2521 gacatggaca ccgtcctcgg ccactaccgg tcgggcgact ggaacgccgc ccgcacctac
     2581 ctgaccgcca ccatcggcac ccgctacgac aacgactggt acacctggct ggcgggctgc
     2641 gcggccggtg actgcggcac cggcaccggc accgggcccg gggccgagtg caccggcacc
     2701 gacaccaggg aactgggccg ggactgccgg cggagcgacc agtccgccac caaggggaac
     2761 tacgcctacc tgtacctcca cgtcccggcc ggcaccaccc ggctgacgat caccacgtcc
     2821 ggcggcacgg gcgacgcgga cctgtactac ggcgacagcg gctggcccgg caccggcggc
     2881 tacacgcggc gtgacaccgg caccggcaac agccacaccc tgaccgtcga caacccggcg
     2941 gccggcaccc actacatcag cctgtacgcg gtggacggct tcaacggggt caccgtcggc
     3001 accgactact gaccggacac ggtcacccgc gcggagacgt ccggcgcggc aaggcccggg
     3061 acggctcgaa gccgccccgg gccgcaggcg tacgcctggc cgctcagagg gtgaaaccgg
     3121 ccgggtaggg gtccgtggga tcgagcaggt actgcgcggt gccggtgatc caggcgcggc
     3181 cggtgacgga cggcagcacg gccgggcggt ccccgaccgt cgtctcgccc aggatccggc
     3241 cgacgaaccg ggagccgatg aacgactcgt tgacgaagtc ccggccggcc gggagcaggc
     3301 cgcgggcgtg cagttgtgcc atccgggcgc tggtgcccgt gccgcagggc gaccggtcga
     3361 accagcccgg gtggatggcc atggcgtgcc gtgagtgctc ggcggtcgat ccgggggctt
     3421 cgaggtagac gtggtggcac acgtcgatct ccgggttctc cgggtgggac accgggttct
     3481 gcttgttgac ggcgtccatg acggccagtc cggcgtcgag cagccgctgc ttgtgcgccc
     3541 gctcgaaggg gatgccgagg tcgtcggtgc gcacgaaggc gtagaagttg ccgccgtagg
     3601 cgaggtcgta gcgcacctgc ccgtagccgg ggacgtcgac gacctggtcg agtgcgtggc
     3661 agtacgacgc cacgttctcc agggtcaccg actcggcgcg gccgtcgcgc acccgcacgc
     3721 gggccgtgac cagtccggcc ggtgtgtcga gccggacccg ggtctccggc tcggtcacct
     3781 cgaccatgcc ggtctcgacg agcaccgtcg cgacaccgat ggtgccgtgg ccgcacatcg
     3841 gcaggcaccc ggagacctcg atgaagagca cgccgtagtc ggcgtccgga cgggtggggg
     3901 gttgcaggat cgccccggac atcgaggcgt ggccgcgcgg ttcgcacatg agcagggtgc
     3961 gcagatggtc gcgttccgcg acgaaccgct gccgcttctc gaacatcgtc gcgccgggga
     4021 gggcgcccac cccgcccgtg atcacgcggg tgggcatgcc ctcggtgtgc gagtcgacgg
     4081 cgtggtagca gaccgtcgag cgcatcgtca gttcaccccc gcgtcgatca gggcctgggt
     4141 ggcggcgcgc acgacggcct ccgtctcggg agccagcggc tgccgcggcg gccggcatcc
     4201 gccaccgcgg cggccggtca gctcctggcc gagcttgatg gcctggacga actccgtctt
     4261 gctgtcccac cgcagcaccg ggtgcagtcg ccggtagagc ggcagtgccg tctccaggtc
     4321 gccccggacg gacgcctcgt acagggcgag gcaggcccgt gggaagacct gcgggtatcc
     4381 ggcgacccag cccttggcgc cggcgagggc gacctcgagg acggtgtcgt cggtgccgat
     4441 catgaggtcg agtccggggg ccagttcgga gatctcgtag cagcgccgga cgtcgccgga
     4501 gaactccttg acgccgacca cgaacccttc ggcgtgcaac ttggccagca ggtcggggcg
     4561 caggtccacc ttggtgtcga tggggttgtt gtacgcggtg acgggcaggc ccaccgaggc
     4621 caccgcctcg aagtgctcga gtaccgcgcg gtcgtcggcg cggtaggcgt tgggcggcag
     4681 gcacatcacc gcctggcagc ccgcgtcctt ggcgaactgg gcgtgccgcc tggcctcctc
     4741 gccgccgtag gcgccgacgc ccggcatgac ggtgaacccg gcgggggcgt gggcgacggc
     4801 ggtctcgacg acgcggtcgc gctcctcgta cgtgagggtc tggtactcgc ccagcgaccc
     4861 gttgggcgcg acgccgtgca tgccctgctc ggcgaggtgg gcgacgctct cgccgtaggc
     4921 gccgaagtcg accgagagat cgtcgtggaa cggcaggctg gtcgcgacga tgacgccgtg
     4981 ccagggcttg cgggtctcgc tcatgaacac tccttgtcgg tggagccgtt gtccgtcggc
     5041 gccgtcggtg cctgtcgtgc ggtcggtgcc tgtggtgtct gcggtgcctg tggtgcctgt
     5101 ggtgcgcgcg gtccgtcgtc gaggtcggcg agcgccccga gggtgacggg tgtcgcgacg
     5161 agccgttccg cgggctcgta ggcccgcccc ggcgccgtga ggcagtgcac ggcgggtccg
     5221 cacatccgcc cctggcacca gcccatgccg gcccgggtca gctgcttgac ctgccggtgg
     5281 tcgtgggcgc cgtcggcacg ggcggcccgg accgcacccg cggtcacctc ctcgcagcgg
     5341 cacaccacgg tctcctcggt gagccacgcg ggccaggcgg gagggaccgg gtgggccagt
     5401 gccatcgccc gggcgaaggc gcgttgccgt accaccgccg aacgcagcgc ggtcgggggc
     5461 cggttcgccg gggcggggct ggtgccggag tcggcgagga ccgaccgcgc ggccagccgg
     5521 ccctcgttca gggcgagcgc cgcgccgccc acaccgcagg tctcccccgc ggtgtagaga
     5581 ccggggacgg tggtgcgctg gtccgcgtcg acggcggcca ccgcgttgcc gtccgccgac
     5641 tctgtaaggg cgcagccgag ggggaccagc aggtccagtt ggggtgtgaa gccccagccg
     5701 acgcccacgg cgtcgacctc gacgcggcgt tccgtacccg ggcgcgggct gccgtcgggc
     5761 gccagcgagg cgatccgcac ggacgacacg cgtgcgccgc cttccgcggc gacgatcgcc
     5821 gtgcgcgggc ggacggggac gcggtggcgg gcgagcgtgg cggcgtatcc ggctccctcg
     5881 gcccacttgc ccgggttgcg cagcaggggg acggggtgcc gcagccaggc ccgcgggtgg
     5941 gccgcctcgc acaccgcgac gacctcgacg ccgcgcgcgg cgagaccggc ggccaccggc
     6001 agcaggaacg gcccggtgcc gcccagcgcc acacggctcc cggccgccac tccgccgccc
     6061 ttgagcagcg cctggagtcc gccggcggtc aggaccccgg gcaggtccca gccggggaag
     6121 ggcagttgcc ggtcgtaggc gccggtggcc acgaggagcc gcggcgcccg caggacgacc
     6181 ggggtctccc gcggcgcgtc gcggcggtcg accgcatgga ccgcgaaacc gtcgccctcg
     6241 cggacggcgg accacgcgtg gtgtccgagg aggaggtcga gccggcccgc cgcccggtgc
     6301 gtcgccaacg cccggcacag ggcacggtag gtacgcagtc cgtggtgcag gtccccggtc
     6361 ggaacggccg ctcgggcgtg ctcgggcggg tgccgccagt actggccgcc gacggtggtg
     6421 ccggagtcca ccagcacgac gcgcagtcct ccggtcagcg ccgtgaccgc ggccgccagt
     6481 ccggccgggc ccgcgcccac gacgacgaga tccgccggct cacgcatcgt cgtcctcccg
     6541 cgtcgtgacg gtcataccgg gccgggccgg caccaggcag gcgcgctgcc cgccggtgcc
     6601 gtcgatggtc aggaggcagt cgaagcagac gccgatgccg cagaagacgc cacgcgggcg
     6661 gtgtccgaca cgggtgctgc gccaggcgac ccggccggcg gcgaccaggg ccgcggcgac
     6721 ggtctgaccc tcgacgaacg gcaacggctc accgtcgacg gtgatctcac tcactggggc
     6781 gcacctccgg gtcgaggaag cggtcgggga gaaggccggt gtgcgcggcc gggtcggcgc
     6841 cgcgccaggg gcggccgagc aggtgggccg tcaccagggc gccggtcgcg ggggcgaggc
     6901 cgatgcccgc gccctcgtga ccgcaggcgt gcaggacgcc cggcacgcgc gggtcggggc
     6961 cgacgaccgg cagatggtcg gggcagtacg gccggaatcc gcggtaggcg cgcatcaggt
     7021 ggactccgcg caggaagggg aagagccggc acgcctgtgc ggcgagcctg gcgaccacgg
     7081 cggtgttcat cgtggtgtcg aagccgacgc gttcccggct ggcgccgacg aggatcgtgc
     7141 cgccgcgcgt gccctccacg acgcaggagg tctccagacc cgcgtcggag gaggcgacat
     7201 tggcgacgta gtcggcggag tacaccttgt gccggaccat cggcggcagc ggttcggtca
     7261 ccaggacgaa gccgcggcgg ggcagcacct cgacgggggc gccgagccgc cggccgacct
     7321 cgccgcccca ggtcccggcg gcgttgacga cggcgtcggc gggcaggacg tcaccggcgg
     7381 ccgtgcggac gccggtgatc cggccgccgt cccgggtggt cacggcgccg gagacctcgc
     7441 cggtgcggaa gcgcgcgccg tgccgtacgg cggctcgcag cagtgcggcc gccgcgagca
     7501 ccggctggac ctgggcgtcc tgcgggtagt ggacgccgcc ggggatcccg ggtgcgaggt
     7561 ggggttcgag gtcccgcacc ctctcgaccg gctcgacgcg gacaccggcc gcctcctgcc
     7621 gggcggcgaa ctcgccgagc gcggtaaggc tttcggcggt gctcgcgacg accaggccgc
     7681 ccttgggctc gagttcgagg gtgtcggggc cgagctcccg gccggcctcg tcccacaggg
     7741 cgcggctcag gtgggccagg gcgagttcgg ggccgggttc cttgtcggag aggaggacgt
     7801 tgccctctcc gcggctcgtc gttccggcgc ccacgggtcc gcggtcgacg accgtgacgt
     7861 ccaggcccgc cgaggcggcg tggtaggcgc aggcggctcc gacgactccc gcgcccacga
     7921 cgacgacctt gagggtcaac ggctcctcct ccgtgcgcca caccgcgtct gtgcaatgtc
     7981 acatgccatg agtgaggcta tacatccggt cggcggaaga caacgccccc ttcgcgcccg
     8041 ggagtcagag atcgactgcg aggtcggccg ggagctcggc tcagaggtcg actcgggtgc
     8101 ccaggccggt cagggcaccg accgggtcga ggtccggggt gccgcgcggc caccagtcct
     8161 cgcggccggg ctccgactcg tacgcgtacc agagtcccga ccggccgagt ctgagctgga
     8221 catggccccg ggggtgggtg aggtggttgc gccaggggcg gaaggcgggg aggtctgcgg
     8281 cgagcagcag ggggcgggcg cggtcgaaac ggccggccgg cgggtcccac ggctcctcca
     8341 ggacgtcgag accctggggt ccgccctgcc gccaggcggc gacggcccgc gccagctcgg
     8401 ccgtgtcgcg cccggcggcc gaggcgagtg acgcgtagag cgcgcgggtg cccgcggtga
     8461 gccctgagcc ggggcgcgcc gcggcgaggc ggacggcgtc ctgccacagc gtcagcgtgc
     8521 cgaccgggtc gcgtccggtg gtgagcaggg cgtgggcgcg ggcggccgcg tcggtggcga
     8581 gctggtccag ggcgaacgga tcgggaccgc cgggggcggc cgggtaggcc gggggctgtt
     8641 cgggatgcgg gggcgcgggc agcggcgccg ggaggggcgg cagggcgcgc cgggccagga
     8701 cctcaccggc ccggagtccg ggcagcggcg ccggtccccg gtcctgggcg gcgcgtgccg
     8761 cgcgggccgc gttccgccgg gacagggcgt cgagcagcgc ccgttcgccg cggccgcgca
     8821 gcaggaggag cacgaagggg tcggcgtcga gcagacgcgc cgtctggtag cagagggctg
     8881 cggcgtgctt gcaggggtgg ccggagtcgg ggcagctgca ctgcggggtg aggtcaccgg
     8941 ggccgggaag cagcggaacg ccgcggtcgg cgaggtcggc cagggagtgg ggaagctcct
     9001 tgtcgagcag ggcggcgatg tgcccgggcc gctccacggc ggcgtccagg aaccggtccc
     9061 agtccgcgtc gccgagcgtg cgcagccgca cctggacgcg gtacggccgg gcgcggctgc
     9121 cctgcacgta ggcgaggacc agcccgggcg tcacggtgat ggcgtccacg tgtcccccct
     9181 cggcgtaccc gcgcccgcgc tccagccgcg cgacgtccag ggcgccctcc tccagcgccc
     9241 gcacccaggc gttgccccac caggtcccgg cgaggccctc gtccgcggcc ctgcggggcg
     9301 gaagcgcggg aaaggtgcgc cggagatcgc cgtcacggcc cggggccgcc atcgagcggg
     9361 gggcgcgggc ttgcgcgacc ggtgcggagt gaggctcccg gccgcccgcc gccgacgggt
     9421 gcgccgggtc ccgcgggtac cgcggacgtc ccgaccgctc gtgggcggac accggtcctt
     9481 cggagctcgc cgtgcgcgag gcgtgacccg gccactcggg gccggacacc cggccttcgg
     9541 agctcgccga gcgcggggga tgccccgacc gctcgggaac gggcgacgcc ccttcaaggc
     9601 tcgccgcgcg cgagacatgc cccggccact cagggccggg gggcaggctt tcgaggctcg
     9661 tcgtacgggc agcatgcccc gaccactcgg gaacgagggg caggctctcg gggctcgtcg
     9721 taggggcggc atgtcccggc cccccggggc caggcgcccc accctcggtg tgcgccgcgc
     9781 gcccagcatg ccccggctcg cccgggccgg gcggcggacc ttcggggctc accgcgtgca
     9841 gggcatgctc cggcgtaggc atcccagggc cagggggcag gctctcgggg ctcgtcgtac
     9901 ggacggcatg ccccggcccc ccggggccag gagccccgcc ctcggtgtgc gccgcgcgcc
     9961 cagcatgccc cggctgtccg gaaccggtcg ccccgccctc ggcgctcgcc gtgtccccgg
    10021 cgttcggggc gaccgtgggc tcggtaacgg gacctgcgtc gggcgacgtg gcccgctccg
    10081 ggtacggggc cgcccagtcc gcgccggagg gaccaccggc cgggctcccg gcagccgggg
    10141 tctccccgcg gagcgtcgcg tccccggccg ggtccgtctc cgggggcagc cggaacgcgt
    10201 ccgcggcgaa gtcccgtacg gcgcgggcgc gtgcgtcggc ggtgtccgcg ccgcccggcc
    10261 ggcgccgacg agcggccgga gggggcgtcc gtcgtgcgct cgccgcgcgc ccgctcgccg
    10321 cttccgcctt cgcctgccgg cgggccgccc gcagcgcctc acgcgccacg tccgccggcc
    10381 ggctgtcgcc ctggtcggcg gagccgggaa gggacgcccc gtcgggtacg gtccggccgg
    10441 ccggctcggt catgtcgtcg gaccggtccg ccttcccgcc cgtggacgtc gatttctccg
    10501 agtcctgcgg ctctccgtcg tctcccgggt cccccgcccc cggggcttcc gggctcgggt
    10561 gtcctcgccc cgtcgaacca tcccggcccc cagggctctg cggacgtgac ggagtgcccg
    10621 gggcgtcgag ggcgtcggta ccgtcctcga cagcgtccgc ccccgcgtgc cgtgcggtcc
    10681 gtgcgctgcg cagcgcggcg cgcgcgatgt ccccggggcg cgacgcaggc cgctccgccg
    10741 atgcccggtc cggacggcag ccctcccgtc cctgggccgg cgccgacggg gccggtgccg
    10801 ccgcctcgcg ctccccgtcc gcgcccgtgc cctcgccgcg ctcgcgcgcc tcccgcaggg
    10861 cgcggcgggc ctcgtcggcg ggccgggggg tcatgacggc ctcctcaggg agacgaggtc
    10921 ggacagttca cgggccgtca gctcagtcag ggccgcctcg ccggagccga ggatcgcgtc
    10981 ggccagggcc cgcttggact ggagcatctc ggcgatgcgg tcctcgaccg tgccctcggt
    11041 gatgagccgg tggacctgga ccggctgggt ctggccgatg cggtaggcgc ggtcggtggc
    11101 ctgctcctcc accgccgggt tccaccagcg gtcgaagtgg acgacgtggc cggcgcgggt
    11161 gaggttcagg ccggtgccgg cggccttcag ggacaggacg aggacagggg tagccccgct
    11221 ctggaagcgg tccaccatcc gctcgcgctc cggcaccggc gtaccgccgt gcagcaggtc
    11281 gaccgggacc gcgcgggtgg ccaggtggga ggtgatgagg cgggccatgc cgacgtactg
    11341 cgtgaagacc agtgccgagc cgtcctcggc gagcaccgtg tccagcagct cgtccagcag
    11401 ggcgagtttg cccgagcggg cggtcatccg gtcggccccg cccggcgagt gctcctcctt
    11461 caggaacaac gcggggtggt cgcagatctg cttcagcgag gtcagcagct tgaggacgag
    11521 gccgcggcgg cccatgccct cggcctcctc gatggcgagc atcgactcgc gcaccaccgc
    11581 ctcgtacagc gcggcctgtt cccgggtgag cgggacgggg tggtccgtct cggtcttcgg
    11641 cggcagctcg gggacgatgc ccgggtcgga cttcctgcgg cgcagcagga aggggcggat
    11701 gaggcgggcg aggcgctcca ccgcctggtc gtcctcgccg ttctccaccg cgcgcgcgtg
    11761 ccgggagcgg aaggacttga gggggccgag cagtccgggg gtggtccagt cgagcagtgc
    11821 ccacagctcg gagaggttgt tctccacggg ggtgccggtg agggccaccc gcgcggggga
    11881 cgggatcgtg cgcagggcct tcgccgtcgc cgagtacggg ttcttcacgt gctgggcctc
    11941 gtccgcgacg accatgcccc agggctgccc ggcgagggtc gtggcggcgg accgcatcgt
    12001 gccgtaggtg gtgaggacga agccgccggt caggtcgtcg agggtgcggt ccgggccgtg
    12061 gaagcggcgg acggggacgc cgggcgcgaa ccgctcgatc tcccgctgcc agttgcccag
    12121 gagggaggcc gggcagacga cgagggtcgg ctcggtgcgg gcccgctcga ggtgcagggc
    12181 gatgacggtg acggtctttc ctaggcccat gtcgtcggcg aggcagccgc cgagaccgag
    12241 cgaggtcatg aggtcgagcc aggccaggcc gcgcagctgg tagtcgcgga gggtggcgtg
    12301 caggccgggc ggtgcctcgg cggggcgcac gcccgccgtg agccggtcgc gcagggcggc
    12361 cagcgcgccg acgggcacca cgtcgaccgt ctcgccgtcg gcctccgcgc tgccggtgag
    12421 ggcgacggag agggcgtcga ccgggtcgag caggcccagg tcgcgcttgc gggcccggcg
    12481 gacgagcgac gggtcgacca gcacccaccg gtcgcggagc cggacgaccg gacggtgggc
    12541 ctccgccagg gtgtccatct cggcctcggt gagcgggtcc ccgccgatcg cgagctgcca
    12601 gcggaactgg agcaggtcct cgctctcgaa gaagcccgtg ccgtcggtcg ccgagccggg
    12661 cgcggtcctg atcacggcgg tcgcggtgat gtcctgggcg aggtcgcggg gccagtgcac
    12721 ggcgacaccc gcggcggaga gccggccggc cgccacgccc agcaggtcgg tgacctcttc
    12781 ctcggacagg gcgagtacgt cggggacgtc ctgctcggtg agccggtcca gcggcgtcca
    12841 gaccctcgct gcccgccgca ccgcgagcgc ggcgtccaca cgcgcgcggg ggccgaacga
    12901 cgcgtcggcc gtccccgacc acaggtccgc ggcatcggc
//
