LOCUS       NZ_JAAHBR010000026      6775 bp    DNA     linear   CON 15-APR-2024
DEFINITION  Bacillus velezensis strain HU-91 NODE_28_length_6655_cov_165.943497,
            whole genome shotgun sequence.
ACCESSION   NZ_JAAHBR010000026
VERSION     NZ_JAAHBR010000026.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus velezensis
  ORGANISM  Bacillus velezensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Ullah,H. and Hassan,M.N.
  TITLE     WGS of Bacillus velezensis HU-91
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Ullah,H. and Hassan,M.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2020) BioSciences, COMSATS University, Park Road
            Islamabad, Islamabad 44000, Pakistan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010671715.1-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 03:53:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,963
            CDSs (total)                      :: 3,845
            Genes (coding)                    :: 3,754
            CDSs (with protein)               :: 3,754
            Genes (RNA)                       :: 118
            rRNAs                             :: 7, 13, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 13, 9 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 45 of 91
            Pseudo Genes (incomplete)         :: 58 of 91
            Pseudo Genes (internal stop)      :: 12 of 91
            Pseudo Genes (multiple problems)  :: 23 of 91
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:14:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6775
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAAHBR010000026.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..2589)
                     /locus_tag="G3T15_RS20005"
                     /old_locus_tag="G3T15_13920"
     CDS             complement(<1..2589)
                     /NRPS_PKS="Domain: AMP-binding (264-665). E-value: 5.5e-99.
                     Score: 323.4. Matches aSDomain:
                     nrpspksdomains_G3T15_RS20005_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (768-837). E-value: 3.2e-24. Score:
                     76.9. Matches aSDomain: nrpspksdomains_G3T15_RS20005_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="G3T15_RS20005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G3T15_13920"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_163069059.1"
                     /sec_met_domain="Condensation (E-value: 1e-24, bitscore:
                     74.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.2e-108, bitscore:
                     349.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.1e-19, bitscore:
                     56.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNMSSKPVFQSSADEGGFNNRKKTFIIDETCTSMLKAAADQYHIT
                     LSALIQTAWGVLLQKYNNVNDVVFGAVISGRQCRVQGIEKMVGLFINAVPLRVKGDGDT
                     VFIALAQKLNSDFIKANTSYGYSSLADIQALTKMKEKLINHMLVYENYPVDDYGKAVDD
                     TTDALRITDMEVFEQTNYDFGLVIIPGSQIKAEMTYNNNVYREDIINRIGVNFCHVLKQ
                     VATDPTRVLKDISAVNEEERGMLQEFNKQSSNYTVNKTIQDLFEERAEKTPDQTAAVYA
                     GKHITYKELNEKANQVATLLMEKGAGKNSITAMMIRPSEYSMIGILGILKTGSAFLPID
                     DESPVSRINHILKDSKADILITDLCFLADKNIAAECLDITDKSIYTSQNTSNPDVQYDL
                     EDEVYVIYTSGTSGKPKGVKVKNKSLVNYSLWVCDQININSDSRSLVTSKYSFDLCYTS
                     IFPVLSGGGQVHFVDKEVYLHSLSLIEYIHKNSITYLKMTPTLFSTLMEDVDMLASCPD
                     LKVIILGGESINIGNVKKLAEKCRWMKFINHYGPTESTVGCIAHSIDLDHIQNCEIYNK
                     IGRPIHGINIYIVDRSDQLVPVGAPGEICISGVGLASGYVNNEELTNEKFIDNPFHPGA
                     KMYKTGDLGRWMPDGNIEFLGRIDNQIKIRGFRVEIGEIENQLLQLEGIKEAAVVYIEE
                     KYLSAYITGDIEMEGSFVQEKLAQALPKYMIPSFIIQLKELPLTANGKINRKELPLPDL
                     QRLSVSRYEAPANETEEKLAEIWKEMLGHKRISINEDFFELGGHSLTAAFTISRIRKTF
                     GTDIKVKELFEQPTIKQLSRLIQKRDKQNYPVISKARKQSYYQASSAQKRM"
     source          1..6775
                     /collected_by="Habib Ullah"
                     /collection_date="2018-04"
                     /country="Pakistan"
                     /db_xref="taxon:492670"
                     /host="Triticum aestivum"
                     /isolation_source="rhizosphere"
                     /mol_type="genomic DNA"
                     /organism="Bacillus velezensis"
                     /strain="HU-91"
                     /submitter_seqid="NODE_28_length_6655_cov_165.943497"
     region          1..6775
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..6775
                     /SMILES="NC(Cc1ccccc1)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6775
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>6775](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>6775)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        complement(79..285)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G3T15_RS20005_PCP.1"
                     /evalue="3.20E-24"
                     /label="G3T15_RS20005_PCP.1"
                     /locus_tag="G3T15_RS20005"
                     /protein_end="837"
                     /protein_start="768"
                     /score="76.9"
                     /tool="antismash"
                     /translation="EEKLAEIWKEMLGHKRISINEDFFELGGHSLTAAFTISRIRKTFG
                     TDIKVKELFEQPTIKQLSRLIQKR"
     aSModule        complement(79..3178)
                     /complete
                     /domains="nrpspksdomains_G3T15_RS13925_Condensation_DCL.1"
                     /domains="nrpspksdomains_G3T15_RS20005_AMP-binding.1"
                     /domains="nrpspksdomains_G3T15_RS20005_PCP.1"
                     /locus_tags="G3T15_RS13925"
                     /locus_tags="G3T15_RS20005"
                     /monomer_pairings="Asp -> Asp"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(565..630)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS20005_0006"
                     /evalue="3.30E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="G3T15_RS20005"
                     /protein_end="675"
                     /protein_start="653"
                     /score="35.2"
                     /tool="antismash"
                     /translation="FLGRIDNQIKIRGFRVEIGEIE"
     aSDomain        complement(595..1797)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G3T15_RS20005_AMP-binding.1"
                     /evalue="5.50E-99"
                     /label="G3T15_RS20005_AMP-binding.1"
                     /locus_tag="G3T15_RS20005"
                     /protein_end="665"
                     /protein_start="264"
                     /score="323.4"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEERAEKTPDQTAAVYAGKHITYKELNEKANQVATLLMEKGAGKN
                     SITAMMIRPSEYSMIGILGILKTGSAFLPIDDESPVSRINHILKDSKADILITDLCFLA
                     DKNIAAECLDITDKSIYTSQNTSNPDVQYDLEDEVYVIYTSGTSGKPKGVKVKNKSLVN
                     YSLWVCDQININSDSRSLVTSKYSFDLCYTSIFPVLSGGGQVHFVDKEVYLHSLSLIEY
                     IHKNSITYLKMTPTLFSTLMEDVDMLASCPDLKVIILGGESINIGNVKKLAEKCRWMKF
                     INHYGPTESTVGCIAHSIDLDHIQNCEIYNKIGRPIHGINIYIVDRSDQLVPVGAPGEI
                     CISGVGLASGYVNNEELTNEKFIDNPFHPGAKMYKTGDLGRWMPDGNIEFLGRIDNQIK
                     IR"
     CDS_motif       complement(712..801)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS20005_0005"
                     /evalue="2.70E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="G3T15_RS20005"
                     /protein_end="626"
                     /protein_start="596"
                     /score="45.2"
                     /tool="antismash"
                     /translation="PVGAPGEICISGVGLASGYVNNEELTNEKF"
     CDS_motif       complement(1330..1380)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS20005_0004"
                     /evalue="2.30E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="G3T15_RS20005"
                     /protein_end="420"
                     /protein_start="403"
                     /score="23.2"
                     /tool="antismash"
                     /translation="YVIYTSGTSGKPKGVKV"
     CDS_motif       complement(1570..1611)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS20005_0003"
                     /evalue="1.90E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="G3T15_RS20005"
                     /protein_end="340"
                     /protein_start="326"
                     /score="11.8"
                     /tool="antismash"
                     /translation="LGILKTGSAFLPID"
     CDS_motif       complement(2131..2223)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS20005_0002"
                     /evalue="5.80E-13"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="G3T15_RS20005"
                     /protein_end="153"
                     /protein_start="122"
                     /score="41.2"
                     /tool="antismash"
                     /translation="SYGYSSLADIQALTKMKEKLINHMLVYENYP"
     CDS_motif       complement(2299..2394)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS20005_0001"
                     /evalue="6.40E-16"
                     /label="C5_DCL_263-294"
                     /locus_tag="G3T15_RS20005"
                     /protein_end="97"
                     /protein_start="65"
                     /score="50.7"
                     /tool="antismash"
                     /translation="DVVFGAVISGRQCRVQGIEKMVGLFINAVPLR"
     gene            complement(2549..>6775)
                     /locus_tag="G3T15_RS13925"
                     /old_locus_tag="G3T15_13925"
     CDS             complement(2549..>6775)
                     /NRPS_PKS="Domain: AMP-binding (155-546). E-value:
                     9.9e-106. Score: 345.7. Matches aSDomain:
                     nrpspksdomains_G3T15_RS13925_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (653-719). E-value: 3.1e-20. Score:
                     64.1. Matches aSDomain: nrpspksdomains_G3T15_RS13925_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (731-1034). E-value:
                     4e-127. Score: 416.9. Matches aSDomain:
                     nrpspksdomains_G3T15_RS13925_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1040-1191). E-value: 6.1e-45.
                     Score: 144.8. Matches aSDomain:
                     nrpspksdomains_G3T15_RS13925_TIGR01720.1"
                     /NRPS_PKS="Domain: Condensation_DCL (1198-1404). E-value:
                     7.9e-68. Score: 220.7. Matches aSDomain:
                     nrpspksdomains_G3T15_RS13925_Condensation_DCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="G3T15_RS13925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G3T15_13925"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_163069060.1"
                     /sec_met_domain="AMP-binding (E-value: 4.7e-116, bitscore:
                     374.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.3e-14, bitscore:
                     41.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 6.8e-75, bitscore:
                     239.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EVKHHTLKDFENTDYQFENIVQKLGVKRDSSRNPLFDVMFTFESK
                     DYGFGGNEETAESNLLVSEVNISRFDLTLTAMETDKGVNFQLEYCNHLFRKDTVERMGA
                     HYTQLLQQVVSEPDTAICRLNMITDDEKHKLLYVFNDTAADFPHNKTIHQLFEEQAEKT
                     PERIAAVYENNKVTYKELNERSNSLARTLMKMGVKKESIVGIVADKSLEMVLGILGVLK
                     AGGAFLPIDPGYPSERIKYMIEDSGSDILLVQNSQADNIPFDGKVIDLTDSSLYSGDRH
                     NLPMMSSAEHLAYVIYTSGTTGKPKGVTIRHIGISNLKSVFEHDMNINYRDKIIQFASF
                     SFDASVWEISMALLTGAELHILSSQVINNYTLFEEYINRHDITVATLPPVYINHINING
                     LSALRLLITAGSQISTKSLNRIVNKIDYINAYGPTETTICSTMWRYSEAERAMAKTIPI
                     GKPIRNLKAFIIDHNNNLVPIGLAGELCVSGDSLARGYLNKEKMTREKFIDHPFESGTK
                     MYRTGDLARWLPDGNIEFLGRIDNQVKIRGFRIETDEIESQLLKISGIKEAVVIDKCDL
                     HGDKYLSAFVTAEYKISGAALREQLGDILPQYMIPGVIIQVEQLPLTPNGKIDRKALAE
                     IEEPKHIKEHLEAPRSEVEQILVNVWSEVLKIDDIGINDNYYELGGDSIKSILIVSKLQ
                     KYSLSLEVKDLLQYPQIKNLSKHVKLYKAQTDQSPVSGRTELAPIQKWFIENDFADIHH
                     FNQAFMFQMKEKINEHALAKTFELLVKHHDALRLIYTAENDEIKQEVRDVQAARDTFTL
                     DIYDLLADTDYKNTIEKLADQLQSGMDLEKGLLMKLGLFRTMRGDYLLIAIHHMVIDGV
                     SWRILLEDMEQVYNNLQKGEPAVLPPKTASFQEWTRKLAEYANGSEIRKELRYWENIEK
                     TKIKEIPRDFNKTDSVYKESRTITVSLTKQLTYKLQRNTNAAYNTQINDILLCSLGMAV
                     KEWTGLDKVLIGLEGHGRENILENISIERTIGWFTSLFPIVLDMEHSDDLPYCIKNTKE
                     YLRRIPYKGIGYGIIKYLTAREHKKDISFTIKPEIGFNYLGEISQKKRAVFQHADISSG
                     TSVSLSNKKLNAIEINSFIMDGQLQLEFNYSTKEFKEETIKTLTESYINRLIDIIQHCE
                     MKKIPEKTPFDYGDLDLSIGDLEQILSSGKEIEKIHSLTPMQSGMLYHALLDPFSQAYF
                     EQLTFSLSGQLDIGRLNTAFNMLIEKYEILRTCFFHEGLSKFKQAVLRERKVKIEFEDI
                     STFSEYQKKVRLKDVQFKDRGTRFDLTNDCLIRVTVLKTDEKEYKLLFSFHHIIMDGWC
                     MSLIMNDIKTIYTSLKEGRETVLQHTEPYSTYLEWLDNQDKSAALEYWGDYLNEYEQQA
                     GIPKFC"
     aSDomain        complement(2561..3178)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G3T15_RS13925_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="7.90E-68"
                     /label="G3T15_RS13925_Condensation_DCL.1"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="1404"
                     /protein_start="1198"
                     /score="220.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="EKIHSLTPMQSGMLYHALLDPFSQAYFEQLTFSLSGQLDIGRLNT
                     AFNMLIEKYEILRTCFFHEGLSKFKQAVLRERKVKIEFEDISTFSEYQKKVRLKDVQFK
                     DRGTRFDLTNDCLIRVTVLKTDEKEYKLLFSFHHIIMDGWCMSLIMNDIKTIYTSLKEG
                     RETVLQHTEPYSTYLEWLDNQDKSAALEYWGDYLNEYEQQAGI"
     CDS_motif       complement(2708..2773)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0013"
                     /evalue="9.40E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="1355"
                     /protein_start="1333"
                     /score="34.1"
                     /tool="antismash"
                     /translation="FHHIIMDGWCMSLIMNDIKTIY"
     CDS_motif       complement(2843..2884)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0012"
                     /evalue="4.20E+01"
                     /label="NRPS-A_a8"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="1310"
                     /protein_start="1296"
                     /score="-2.4"
                     /tool="antismash"
                     /translation="KDVQFKDRGTRFDL"
     CDS_motif       complement(2990..3106)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0011"
                     /evalue="4.40E-15"
                     /label="C2_DCL_024-062"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="1261"
                     /protein_start="1222"
                     /score="47.9"
                     /tool="antismash"
                     /translation="AYFEQLTFSLSGQLDIGRLNTAFNMLIEKYEILRTCFFH"
     CDS_motif       complement(3131..3166)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0010"
                     /evalue="9.10E-04"
                     /label="C1_dual_004-017"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="1214"
                     /protein_start="1202"
                     /score="12.2"
                     /tool="antismash"
                     /translation="SLTPMQSGMLYH"
     aSDomain        complement(3200..3652)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G3T15_RS13925_TIGR01720.1"
                     /evalue="6.10E-45"
                     /label="G3T15_RS13925_TIGR01720.1"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="1191"
                     /protein_start="1040"
                     /score="144.8"
                     /tool="antismash"
                     /translation="YCIKNTKEYLRRIPYKGIGYGIIKYLTAREHKKDISFTIKPEIGF
                     NYLGEISQKKRAVFQHADISSGTSVSLSNKKLNAIEINSFIMDGQLQLEFNYSTKEFKE
                     ETIKTLTESYINRLIDIIQHCEMKKIPEKTPFDYGDLDLSIGDLEQI"
     aSModule        complement(3200..6307)
                     /complete
                     /domains="nrpspksdomains_G3T15_RS13925_AMP-binding.1"
                     /domains="nrpspksdomains_G3T15_RS13925_PCP.1"
                     /domains="nrpspksdomains_G3T15_RS13925_Epimerization.1"
                     /domains="nrpspksdomains_G3T15_RS13925_TIGR01720.1"
                     /locus_tags="G3T15_RS13925"
                     /monomer_pairings="Phe -> D-Phe"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(3575..3637)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0009"
                     /evalue="4.00E-08"
                     /label="NRPS-E6"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="1066"
                     /protein_start="1045"
                     /score="25.6"
                     /tool="antismash"
                     /translation="TKEYLRRIPYKGIGYGIIKYL"
     aSDomain        complement(3671..4579)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G3T15_RS13925_Epimerization.1"
                     /evalue="4.00E-127"
                     /label="G3T15_RS13925_Epimerization.1"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="1034"
                     /protein_start="731"
                     /score="416.9"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHMVIDG"
                     /tool="antismash"
                     /translation="GRTELAPIQKWFIENDFADIHHFNQAFMFQMKEKINEHALAKTFE
                     LLVKHHDALRLIYTAENDEIKQEVRDVQAARDTFTLDIYDLLADTDYKNTIEKLADQLQ
                     SGMDLEKGLLMKLGLFRTMRGDYLLIAIHHMVIDGVSWRILLEDMEQVYNNLQKGEPAV
                     LPPKTASFQEWTRKLAEYANGSEIRKELRYWENIEKTKIKEIPRDFNKTDSVYKESRTI
                     TVSLTKQLTYKLQRNTNAAYNTQINDILLCSLGMAVKEWTGLDKVLIGLEGHGRENILE
                     NISIERTIGWFTSLFPIVLDME"
     CDS_motif       complement(3680..3727)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0008"
                     /evalue="1.40E-06"
                     /label="NRPS-E5"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="1031"
                     /protein_start="1015"
                     /score="20.6"
                     /tool="antismash"
                     /translation="IERTIGWFTSLFPIVL"
     CDS_motif       complement(4133..4198)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0007"
                     /evalue="4.20E-11"
                     /label="NRPS-E2"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="880"
                     /protein_start="858"
                     /score="35.0"
                     /tool="antismash"
                     /translation="LLIAIHHMVIDGVSWRILLEDM"
     CDS_motif       complement(4412..4510)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0006"
                     /evalue="1.40E-04"
                     /label="C2_LCL_024-062"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="787"
                     /protein_start="754"
                     /score="14.3"
                     /tool="antismash"
                     /translation="NQAFMFQMKEKINEHALAKTFELLVKHHDALRL"
     aSDomain        complement(4616..4813)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G3T15_RS13925_PCP.1"
                     /evalue="3.10E-20"
                     /label="G3T15_RS13925_PCP.1"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="719"
                     /protein_start="653"
                     /score="64.1"
                     /tool="antismash"
                     /translation="EQILVNVWSEVLKIDDIGINDNYYELGGDSIKSILIVSKLQKYSL
                     SLEVKDLLQYPQIKNLSKHVK"
     CDS_motif       complement(5105..5170)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0005"
                     /evalue="3.60E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="556"
                     /protein_start="534"
                     /score="35.0"
                     /tool="antismash"
                     /translation="FLGRIDNQVKIRGFRIETDEIE"
     aSDomain        complement(5135..6307)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G3T15_RS13925_AMP-binding.1"
                     /evalue="9.90E-106"
                     /label="G3T15_RS13925_AMP-binding.1"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="546"
                     /protein_start="155"
                     /score="345.7"
                     /specificity="substrate consensus: Phe"
                     /tool="antismash"
                     /translation="FEEQAEKTPERIAAVYENNKVTYKELNERSNSLARTLMKMGVKKE
                     SIVGIVADKSLEMVLGILGVLKAGGAFLPIDPGYPSERIKYMIEDSGSDILLVQNSQAD
                     NIPFDGKVIDLTDSSLYSGDRHNLPMMSSAEHLAYVIYTSGTTGKPKGVTIRHIGISNL
                     KSVFEHDMNINYRDKIIQFASFSFDASVWEISMALLTGAELHILSSQVINNYTLFEEYI
                     NRHDITVATLPPVYINHININGLSALRLLITAGSQISTKSLNRIVNKIDYINAYGPTET
                     TICSTMWRYSEAERAMAKTIPIGKPIRNLKAFIIDHNNNLVPIGLAGELCVSGDSLARG
                     YLNKEKMTREKFIDHPFESGTKMYRTGDLARWLPDGNIEFLGRIDNQVKIR"
     CDS_motif       complement(5252..5341)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0004"
                     /evalue="1.50E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="507"
                     /protein_start="477"
                     /score="39.7"
                     /tool="antismash"
                     /translation="PIGLAGELCVSGDSLARGYLNKEKMTREKF"
     CDS_motif       complement(5837..5896)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0003"
                     /evalue="8.80E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="312"
                     /protein_start="292"
                     /score="30.5"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVTIRH"
     CDS_motif       complement(6080..6121)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0002"
                     /evalue="6.60E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="231"
                     /protein_start="217"
                     /score="16.2"
                     /tool="antismash"
                     /translation="LGVLKAGGAFLPID"
     CDS_motif       complement(6647..6760)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_G3T15_RS13925_0001"
                     /evalue="7.50E-14"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="G3T15_RS13925"
                     /protein_end="42"
                     /protein_start="4"
                     /score="44.0"
                     /tool="antismash"
                     /translation="HTLKDFENTDYQFENIVQKLGVKRDSSRNPLFDVMFTF"
ORIGIN
        1 cattcttttc tgagcagatg atgcttgata ataagactgc ttcctcgctt tactgatgac
       61 cggataattc tgtttatccc gcttttgaat aagacggctc agctgtttga tggtaggctg
      121 ttcaaaaagc tccttcacct ttatatccgt accgaaagtt tttctgatgc gggaaatagt
      181 gaacgcagct gttaaagaat gtccgcccag ttcgaagaaa tcttcattta tactgatccg
      241 tttatgccca agcatttctt tccaaatttc agctagtttt tcttccgttt catttgccgg
      301 agcttcgtat cgagatactg acaaccgttg caagtcagga agaggcagtt cttttcgatt
      361 gatttttccg ttagctgtaa gaggcagctc tttcagttgt attatgaaac tcggaatcat
      421 atacttcgga agagcctgtg ccaatttctc ctgtacgaac gagccttcca tctcaatgtc
      481 cccagtaata tatgcagaaa gatatttctc ctctatataa acaaccgcgg cttcttttat
      541 accttccagc tgtaatagtt ggttttcgat ctcaccgatt tcaactctaa aacccctgat
      601 tttgatctgg ttgtcaatcc ggcctaaaaa ttctatattt ccatccggca tccatctgcc
      661 aaggtctcct gttttgtaca tttttgctcc gggatgaaaa ggattatcaa taaatttttc
      721 attcgttaat tcttcattat tgacatatcc cgatgctaaa cctaccccgc tgatacaaat
      781 ctcaccgggt gctcccacag ggaccagttg atctgaacga tccacaatat agatattaat
      841 gccgtgaatt ggtcttccta ttttattgta tatttcacag ttttgaatat gatctagatc
      901 aatgctatgt gcaatacagc caacagtgct ttcggtcggc ccgtaatggt taataaactt
      961 catccatctg catttttcag cgagcttttt gacgttgccg atgtttatgc tttcgccgcc
     1021 cagtatgata acttttaaat caggacaaga cgccagcatg tcaacatcct ccatcaatgt
     1081 tgaaaataaa gtaggtgtca ttttaagata tgtaatacta tttttatgaa tatattctat
     1141 taatgataga gagtgaagat acacttcttt atcaacaaag tggacttgtc cgccaccgga
     1201 taaaaccgga aatatactgg tatagcataa gtcaaaacta tattttgacg tcactaacga
     1261 cctgctgtca gaatttatgt ttatttgatc acatacccat aatgaataat taactaatga
     1321 tttatttttc accttcaccc ccttcggttt tcccgaagta cccgaggtat atataacata
     1381 tacctcgtcc tcgagatcat actgaacatc agggtttgat gtattttggc tggtataaat
     1441 cgatttatcc gtaatatcca aacattccgc cgcaatgttt ttatcagcta aaaagcacaa
     1501 atctgtaatg aggatatcag ctttgctatc ttttaaaata tgatttatcc gactgaccgg
     1561 tgattcatca tcaataggca gaaacgcact tccggttttt aaaattccca gtatcccaat
     1621 cattgaatat tctgaaggac ggatcatcat agccgtaatg ctgttttttc ctgccccttt
     1681 ttccattaat aatgttgcaa cttgattagc cttttcgttt agctctttat aagtgatatg
     1741 ctttcctgca tagacagccg ctgtttggtc aggtgtcttt tccgctcgtt cttcaaaaag
     1801 gtcttgtatc gttttattca cggtataatt agaggactgc ttattaaact cctggagcat
     1861 tccgcgctct tcttcattta cagcgctgat gtctttcagg acacgcgtag gatcagttgc
     1921 cacttgcttt aatacatgac agaaattaac acctatacga ttaatgatat cttcacgata
     1981 aacattattg ttatacgtca tctctgcttt gatttggctg cctggaataa taaccagccc
     2041 aaagtcatag ttcgtctgtt caaacacttc catatctgta atccttaaag catcagttgt
     2101 atcatcgacc gccttgccat agtcatcaac agggtaattt tcataaacca gcatatggtt
     2161 gatcagtttc tctttcatct ttgtaagagc ttgtatatcc gctaaagaac tgtaaccgta
     2221 tgatgtattc gctttgataa agtcactgtt cagcttttgg gctaatgcta taaataccgt
     2281 atcaccatcg ccttttactc tcagaggaac cgcattgata aataacccca ccattttttc
     2341 tatgccttgc actctgcatt gtcttccgga aataaccgca ccaaacacaa catcattcac
     2401 gttgttatac ttctgcaaaa gaactcccca tgcagtctga atcaatgcac ttaaagtaat
     2461 gtgatattga tcagctgcag ctttcaacat cgaagtacat gtttcatcaa taatgaatgt
     2521 tttcttccta ttattgaaac cgccctcatc agcagaactt tggaataccg gcttgctgct
     2581 catattcatt tagataatcc ccccaatatt ctaatgcggc acttttgtcc tgattatcca
     2641 gccattctaa gtaggtgctg taaggctcgg tatgctgaag cactgtctct cttccttctt
     2701 ttaatgacgt ataaatagtt tttatatcat tcattattaa actcatacac cagccatcca
     2761 taataatatg gtgaaaactg aacagcagtt tatattcttt ctcatcagtt tttaatacgg
     2821 ttactcttat taagcaatca ttcgtcaaat cgaatctcgt tcccctgtct ttaaactgga
     2881 catctttaag ccggactttc ttttgatatt ccgagaaagt gctgatatct tcaaattcaa
     2941 ttttaacttt tctctctctt agaacagcct gtttaaactt acttagtcct tcatgaaaaa
     3001 agcatgttct taatatttca tacttctcga tcagcatatt aaaagctgta tttaatctgc
     3061 caatatcaag ctgaccggat aaagaaaatg taagctgttc aaaataggct tgtgaaaacg
     3121 gatcaagcaa tgcatgataa agcatcccgc tctgcatagg agtcagggag tggattttct
     3181 ctatctcttt tccgcttgat aaaatctgct ccaaatctcc aatggataaa tccagatctc
     3241 catagtcaaa gggtgttttt tcaggaattt ttttcatttc gcaatgctga ataatatcga
     3301 ttaaccggtt tatataggat tcggttaatg tcttaattgt ttcttcttta aactctttcg
     3361 tactatagtt aaattccaac tgcagttgac cgtccataat aaagctgttg atttcgatgg
     3421 cattcaattt tttattagat aaagaaacac ttgtaccaga tgatatatct gcatgctgaa
     3481 aaacagcccg ctttttttga gagatttctc ccagatagtt aaacccgatc tcgggcttta
     3541 tcgtaaaact gatgtctttt ttatgttccc gtgccgtcaa gtatttaata attccgtaac
     3601 caataccttt ataaggtatt cttcttaaat actctttcgt attttttatg caataaggca
     3661 gatcatcact gtgctccata tccagcacga tcggaaatag tgaggtgaac cagccgatgg
     3721 ttctttctat tgaaatgttt tctaatatgt tctctcttcc gtggccttcc agaccgatta
     3781 aaaccttgtc aagtcccgtc cattctttta ctgccattcc cagtgaacaa agcaagatgt
     3841 cattaatctg tgtgttataa gctgcatttg tattcctctg gagcttataa gttaactgct
     3901 ttgtaagact gacagtaatg gtgcggcttt ccttatatac actgtcagtt ttattgaaat
     3961 ctctcggaat ttctttaatc ttcgtttttt cgatattctc ccaataccga agttcctttc
     4021 ttatctcact tccgtttgca tattccgcca atttacgtgt ccattcttga aaggatgccg
     4081 ttttcggcgg aagaacggcc ggctctcctt tttgcagatt gttgtagacc tgctccatat
     4141 cttcaagcag aattctccaa gagactccgt caatcaccat atgatgtata gcaataagta
     4201 gataatcgcc cctcattgtt cgaaataaac caagtttcat taaaagccct ttttcaagat
     4261 ccattccgct ttgtaactga tcggcaagtt tttctattgt atttttgtaa tccgtatcag
     4321 caagcagatc atatatgtcc agagtaaaag tatcccgggc ggcttggaca tctctgactt
     4381 cttgttttat ttcgtcattt tcggctgtat atatcaacct caaagcatca tgatgcttta
     4441 ctaataattc aaatgtcttt gccagagcgt gttcatttat tttttctttc atttgaaaca
     4501 tgaatgcctg attaaagtgg tgtatgtccg caaagtcatt ctctataaac cacttttgta
     4561 tcggtgccag ttcagttctc ccgctaaccg ggctctgatc tgtttgagct ttatataatt
     4621 tcacgtgttt actgaggttt tttatttgcg gatactgcaa aagatctttt acctctagac
     4681 tgagactgta tttttgaagc tttgatacaa tcagtataga ttttattgag tctccgccca
     4741 gctcatagta attatcatta ataccaatat catcaatttt cagaacttcc gaccaaacat
     4801 ttaccagaat ctgttcgact tcggaacggg gtgcttcaag gtgctccttt atatgtttag
     4861 gctcttctat ttcagcaagt gctttcctgt caattttccc atttggtgtg agaggcagct
     4921 gttcaacctg gatgatgaca ccgggaatca tatattgcgg caaaatgtca cccaattgct
     4981 ccctcaaagc tgctccggat attttatatt ctgcggttac aaaagcagaa aggtatttgt
     5041 caccgtgaag gtcgcactta tctataacaa ctgcttcttt tatcccgctt attttcagaa
     5101 gctggctttc tatttcatca gtttcaatcc ggaagcctct gattttgact tgattatcaa
     5161 ttctccccaa aaattctatg tttccgtcag gaagccacct tgccaagtct cctgttcggt
     5221 acattttagt tccggactca aatggatgat caataaactt ctcacgcgtc attttctctt
     5281 tattgagata tcctcttgct aagctgtcac ccgacacaca caattctccc gctaaaccaa
     5341 taggcacaag attgttgttg tgatcaatta tgaaggcttt cagatttctg atcggcttac
     5401 cgataggaat ggtctttgcc atcgccctct cagcttctga gtatctccac attgtagagc
     5461 agattgtcgt ttcagttgga ccgtaggcat taatatagtc tattttattc acgatccggt
     5521 tcaaggattt tgtagatatc tgagaaccag cagtaattaa gagccttaaa gctgataatc
     5581 cgtttatatt tatatggttg atgtatacag gcggcaaagt ggctacagta atgtcatgcc
     5641 ggttaatata ctcttcaaat aatgtataat tattgatgac ttgacttgaa aggatgtgta
     5701 gttccgctcc ggtcaacagt gccatactga tttcccacac tgatgcatca aatgaaaaac
     5761 ttgcaaattg aataatttta tcccggtaat tgatattcat atcatgttca aagacacttt
     5821 tgagattact gattccgata tgccggatcg ttacaccctt tggttttcca gtcgttccag
     5881 aagtataaat gacataagca agatgttctg cggaactcat cattggcagg ttatgccgat
     5941 ctccggagta aagagaacta tctgttaaat caataacctt cccgtcaaac gggatattat
     6001 cagcctgact attctgcact aataaaatgt cagaaccgct gtcttcaatc atgtatttga
     6061 ttctctcaga aggatatccc ggatcaattg gcaaaaatgc gcctcccgcc ttcagcacac
     6121 ccaatatgcc taacaccatc tctaatgatt tgtcagcaac aatacctact attgactctt
     6181 ttttcacccc cattttcatt agtgttctcg ccagactatt tgatctttcg ttaagttctt
     6241 tataagttac tttattattt tcatacactg ccgctattct ttccggtgtc ttttccgctt
     6301 gttcttcaaa caattgatgg attgtcttat tatgcgggaa gtcggctgct gtatcattga
     6361 atacataaag cagtttatgt ttctcatcat ccgttatcat attcagtctg cagattgccg
     6421 tgtccggttc tgatacaacc tgttgtaaaa gctgggtata atgagcaccc attctttcga
     6481 ctgtgtcttt cctgaataaa tgattgcaat attccaattg gaagttaacg cctttatcgg
     6541 tttccattgc agttaacgtt aaatcaaatc ttgatatatt gacctcagat actagtaaat
     6601 ttgattctgc tgtctcttca tttcctccga atccgtaatc cttactctca aaagtaaaca
     6661 tgacatcaaa cagcgggttc cggctcgaat ctctttttac cccgagtttt tgaacaatat
     6721 tttcaaattg gtaatctgta ttctcaaaat cttttaacgt atgatgtttg acctc
//
