LOCUS       NZ_NBBO01000004        10387 bp    DNA     linear   CON 10-JUL-2024
DEFINITION  Bacillus subtilis strain TS4 scaffold_4, whole genome shotgun
            sequence.
ACCESSION   NZ_NBBO01000004
VERSION     NZ_NBBO01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 202880)
  AUTHORS   Kaul,S., Sharma,T. and Dhar,M.K.
  TITLE     Whole genome sequence of Bacillus subtilis, an endophyte of Crocus
            sativus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 202880)
  AUTHORS   Kaul,S., Sharma,T. and Dhar,M.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2017) School of Biotechnology, University of
            Jammu, B. R. Ambedkar Road, Jammu, J&K 180006, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 96.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010634645.1-RS_2024_07_10
            Annotation Date                   :: 07/10/2024 04:09:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,571
            CDSs (total)                      :: 4,476
            Genes (coding)                    :: 4,266
            CDSs (with protein)               :: 4,266
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 144 of 210
            Pseudo Genes (incomplete)         :: 72 of 210
            Pseudo Genes (internal stop)      :: 24 of 210
            Pseudo Genes (multiple problems)  :: 27 of 210
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:42:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 34155
            Orig. end                         :: 44542
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NBBO01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10387
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10387
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10387
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5387](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5387)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            230..1114
                     /gene="ycgR"
                     /locus_tag="B6K89_RS02060"
                     /old_locus_tag="B6K89_02080"
     CDS             230..1114
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /gene="ycgR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388207.1"
                     /locus_tag="B6K89_RS02060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6K89_02080"
                     /product="permease"
                     /protein_id="WP_163189219.1"
                     /transl_table=11
                     /translation="MTAQSSFLQLNSIFISILIEAIPFILIGVILSGIIQMFVSEEMIA
                     RIMPKNRFLAVLFGALAGVLFPACECGIIPITRRLLLKGVPLHAGVAFMLTAPIINPIV
                     LFSTYIAFGNRWSVVFYRGGLALAVSLIIGVILSYQFKDNQLLKPDEPGHHHHYHGTLL
                     QKLGGTLRHAIDEFFSVGKYLIIGAFIAAAMQTYVKTSTLLAIGQNDVSSSLVMMGLAF
                     VLSLCSEVDAFIASSFSSTFSLGSLIAFLVFGAMVDIKNLLMMLAAFKKRFVFLLITYI
                     VVIVLAGSLLVKG"
     gene            1119..1976
                     /gene="ycgQ"
                     /locus_tag="B6K89_RS02065"
                     /old_locus_tag="B6K89_02085"
     CDS             1119..1976
                     /codon_start=1
                     /gene="ycgQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388206.1"
                     /locus_tag="B6K89_RS02065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6K89_02085"
                     /product="TIGR03943 family protein"
                     /protein_id="WP_161619478.1"
                     /transl_table=11
                     /translation="MFRLLVLMGFTFFFYHLHASGNLTKYINMKYSYLSFIAIFLLAIL
                     TAVQAYLFIKSPEKSGHHHDHDCGCGHDHEHDHEQNKPFYQRYLIYVVFLFPLVSGIFF
                     PIATLDSSIVKTKGFSFKAMESGDHYSQTQYLRPDASLYYAQDSYDKQMKQLFNKYSSK
                     KEISLTDDDFLKGMETIYNYPGEFLGRTIEFHGFAYKGNAINKNQLFVLRFGIIHCIAD
                     SGVYGMLVEFPKDMDIKDDEWIHIKGTLASEYYQPFKSTLPVVKVTDWNTIKKPDDPYV
                     YRGY"
     gene            complement(2065..2709)
                     /locus_tag="B6K89_RS02070"
                     /old_locus_tag="B6K89_02090"
     CDS             complement(2065..2709)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328050.1"
                     /locus_tag="B6K89_RS02070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6K89_02090"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_042977240.1"
                     /transl_table=11
                     /translation="MSYVMELQNIRKAYGKHVVLENVNIVIEKGEMVAITGSSGSGKTT
                     ILNIMGMLEKPDGGIVKILGDVAPRIGSGRANELLRSKISYLFQNYALIDNASISANLD
                     VPLTYKKLKREEKEKLKIAVLEKVGLQKVSLTQKVHELSGGEQQRLAIARIILKPCDII
                     LADEPTGSLDVKNRNEIITLLKELNQMNKTIIIVTHDPYVSNQCSRVIELN"
     gene            complement(2714..4921)
                     /locus_tag="B6K89_RS02075"
                     /old_locus_tag="B6K89_02095"
     CDS             complement(2714..4921)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328049.1"
                     /locus_tag="B6K89_RS02075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6K89_02095"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_042977242.1"
                     /transl_table=11
                     /translation="MRKIVILFLVISSLFSFLITFRETANKQIEEIEQLDSSVGKPFTI
                     PDDPLLNDPKEMIELIMSSSKKYKTNIFRPATNFSTNDKVEISKYVLLTQQTDYFNGIE
                     LSQGKFLSKLDTQSENFYLSTHKSKDKNQIGQIEKFGITSEITIKPLKNSFDYLPVNGT
                     YYTEIKGKYSFEDFLKGFVNEINEKFDSTFEINDFLQTREVNDSYAEISTSSLQQYNYL
                     ILIVTLFLLIYYIVNESKTIGVLKLHGYSNLRIWFQIVGKLLILTFCFSLLLSIICAIF
                     LPGSTFSFIMNTIMKQIINYLIIGSLSILTYFYIRKVKVNLAIKNKKDTNSIFIFNSIL
                     KIVCSVLIIVIGSSTLNQYSEVKKKQSHLEDWKVSKDYGVFNPLYIGHDIEDVNNGKPQ
                     LTNNIDNIYPILNKMGALLIDTREYEEEFIRLNSSYKGIRSIVVNDNYLKQFPVYNSEK
                     QPISINEREKRLILLVPEKYKQHREKIIKFFKEDRKESAVAYDEEFLGRHVPKYLKEPK
                     IHIIWTASGQKIFSFNPEVFKAEDNLIIDPIIQVVTENNSLVGDRNTILGNGSRDPLKI
                     KLNNRDSKKTMNILVPYLKKYHLDDNLKYLVSLNQYALNEIKELHSEISSLLALAAVLA
                     VALVLLIVQNSVIYFNKKQQKFVVYRLFGIGFFRTFKDYMTLFFILWLIQFISCIVIKR
                     GITLNLLICIVFLLILELVTSIIALIIIERRNKIKVMKGGF"
     misc_feature    complement(4895..4911)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.44"
                     /tool="antismash"
     gene            complement(5001..5387)
                     /locus_tag="B6K89_RS02080"
                     /old_locus_tag="B6K89_02100"
     CDS             complement(5001..5387)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328048.1"
                     /locus_tag="B6K89_RS02080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6K89_02100"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_042977243.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.6e-08,
                     bitscore: 28.0, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKKGLMSAALIAALALSTGQAASAAGAATSGEVDLTNTPLKNSE
                     VTPFANENVGGGNWDYGTAGYFDWGFKKKVWSNYYHKDKAHASSCALGTRTNSSGKTKA
                     GTTSYSDLSGGATDAKTKANWKTY"
     misc_feature    complement(5423..5437)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.52"
                     /tool="antismash"
     misc_feature    5424..5438
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.21"
                     /tool="antismash"
     gene            complement(5579..6808)
                     /gene="putR"
                     /gene_synonym="ycgP"
                     /locus_tag="B6K89_RS02085"
                     /old_locus_tag="B6K89_02105"
     CDS             complement(5579..6808)
                     /codon_start=1
                     /gene="putR"
                     /gene_synonym="ycgP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328047.1"
                     /locus_tag="B6K89_RS02085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6K89_02105"
                     /product="proline utilization transcriptional regulator
                     PutR"
                     /protein_id="WP_042977244.1"
                     /transl_table=11
                     /translation="MEELLERVFSFSDVDKLIDFISYELQKPVILESADFFLLAYNSYY
                     INHFDSANQQTIFSKKCPVQIFERFLKDGIIEKLKTEPEPFRVNKIESIGLNQRVVVSA
                     KHKGEVMGYIWIQELDQNLTDEELDFLYETSFHVGKIIYKTNKLKQEKEEKAEDLIKRA
                     IYQQFTSEKELRREAERINTVLPSMFSVVILHAANGDGEAVEDLKENIRSYLNLRDKVS
                     HVLTIESNIVIVVASFSQKSSVSSAASEFINKLLTHFHFQKIPTPIYIGIGNEYNHLLK
                     LGKSYTEALEVIKAAEITGNQENIPYEYAKLGIYRYLESIEQKNEFLEYENKDLALLKA
                     KDEESSTELLKTLEIYLLNNCKTKPAAEQLFIHQNTLNYRIKQITEMTSIDLSDFRTRC
                     QLYLDLMLMK"
     gene            complement(6961..8382)
                     /gene="putP"
                     /locus_tag="B6K89_RS02090"
                     /old_locus_tag="B6K89_02110"
     CDS             complement(6961..8382)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA]"
                     /GO_function="GO:0031402 - sodium ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /GO_process="GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /function="16.1: Circulate"
                     /gene="putP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388204.2"
                     /locus_tag="B6K89_RS02090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6K89_02110"
                     /product="sodium/proline symporter PutP"
                     /protein_id="WP_010886399.1"
                     /transl_table=11
                     /translation="MLLIGYFAYKRTSNLTDYMLGGRSLGPAVTALSAGAADMSGWLLM
                     GLPGAMFSTGLSGAWIVIGLCLGAWANWLYVAPRLRTYTEKAGNSITIPGFLENRFGDQ
                     TKLLRLFSGIVILVFFTFYVSSGMVSGGVLFNSILGMDYHTGLWIVTGVVVAYTLFGGF
                     LAVSWTDFVQGIIMFAALILVPIVTFFHTGGAGDTVAEIRSVDPDMFNIFKGTSVLGII
                     SLFAWGLGYFGQPHIIVRFMAITSVKEIKRARRIGMGWMILSAVGAVLTGLGGIAYYHQ
                     RGMTLKDPETIFIQLGNILFHPIITGFLISAILAAIMSTISSQLLVTSSSLVEDLYKSM
                     FRRSASDKELVFLGRLAVLAVSIVALVLAWEKNNTILGLVSYAWAGFGASFGPVVLLSL
                     FWKRMTKWGALAGMIVGAATVIIWANAGLSDFLYEMIPGFAASLLSVFFVSILTQAPSQ
                     AVTDQFNDYQDTMSQ"
     gene            complement(8506..10053)
                     /gene="pruA"
                     /locus_tag="B6K89_RS02095"
                     /old_locus_tag="B6K89_02115"
     CDS             complement(8506..10053)
                     /EC_number="1.2.1.88"
                     /GO_function="GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA]"
                     /GO_process="GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pruA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388203.2"
                     /locus_tag="B6K89_RS02095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B6K89_02115"
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="WP_144531492.1"
                     /sec_met_domain="Aldedh (E-value: 3.1e-155, bitscore:
                     510.7, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPYKHEPFTNFQDQNNVEAFKKALATVSEYLGKDYPLVINGER
                     VETEAKIVSINPADKEEVVGRVSKASQEHAEQAIQAAAKAFEEWRYTSPEERAAVLFRA
                     AAKVRRRKHEFSALLVKEAGKPWNEADADTAEAIDFMEYYARQMIELAKGKPVNSREGE
                     KNQYVYTPTGVTVVIPPWNFLFAIMAGTTVAPIVTGNTVVLKPASATPVIAAKFVEVLE
                     ESGLPKGVVNFVPGSGAEVGDYLVDHLKTSLITFTGSREVGTRIFERAAKVQPGQQHLK
                     RVIAEMGGKDTVVVDEDADIELAAQSIFTSAFGFAGQKCSAGSRAVVHEKVYDQVLERV
                     IEITESKVTAKPDSADVYMGPVIDQGSYDKIMSYIEIGKQEGRLVSGGTGDDSKGYFIK
                     PTIFADLDPKARLMQEEIFGPVVAFCKVSDFDEALEVANNTEYGLTGAVITNNRKHIER
                     AKQEFHVGNLYFNRNCTGAIVGYHPFGGFKMSGTDSKAGGPDYLALHMQAKTISEMF"
ORIGIN
        1 tgcacatcat gcaaaagagc acattcgtgg cgctgttctc tgtttgcttc actcatgggg
       61 acacctgatt tggctcggaa aagaagctgc agaaacagac agtatcctat tcggcttttt
      121 ggaatcctaa ccaaagtgaa acgatccttt cccctcctcc gtcaaacaag cagtgccttt
      181 ttttctggta tcataagaaa ggcagcttaa catgagcgga ggttttattg tgacagcaca
      241 atcatctttt cttcagctga attctatttt tatcagcatt ttaatagaag ccattccgtt
      301 tattctgatt ggcgtcatac tttccggaat cattcaaatg tttgtttctg aagaaatgat
      361 tgcaagaatc atgccgaaaa accgttttct cgccgtttta ttcggtgcgc tggccggcgt
      421 tttatttccc gcctgcgaat gcggcatcat cccgattacg aggcggcttc tgttaaaggg
      481 agttcctctg catgcgggcg ttgctttcat gctgaccgcg cctatcatta atcctatcgt
      541 tttattttct acttatattg cgttcgggaa tagatggagc gttgtctttt acagaggcgg
      601 actggctctc gccgtttcgc tgatcatcgg ggtcatctta tcctatcaat ttaaggataa
      661 ccagcttttg aagcctgatg aacccgggca ccaccatcat tatcacggca ccttgctcca
      721 aaaactgggc gggaccttgc ggcatgccat tgatgaattt ttctccgtgg gaaaatattt
      781 aatcatcggc gcctttatcg cagccgctat gcagacgtat gtcaaaacat ccactctgct
      841 tgcgatcggg caaaatgatg tatcctcttc cctcgtgatg atgggtctgg cgtttgtcct
      901 gtcgctttgt tcagaagttg atgcgttcat cgcgtcttcc ttcagcagca cattttcatt
      961 aggatcgtta atcgcctttc tcgtctttgg cgcaatggtg gatattaaga atctattgat
     1021 gatgcttgcg gccttcaaaa aacgatttgt tttcctgctg attacataca ttgtcgtgat
     1081 tgttttagcg ggatcactac tggtaaaggg gtgattgggt gtttcgtcta ttggtgctga
     1141 tgggatttac gtttttcttt tatcatctgc atgcctcagg aaatctaaca aaatatatca
     1201 atatgaaata ttcctatctg tcctttattg cgatcttcct gctggccatt ttgaccgccg
     1261 tccaagcata tctatttatc aagtcgcctg aaaaaagcgg gcatcaccat gaccatgatt
     1321 gcgggtgcgg ccatgatcac gagcatgacc atgaacaaaa caagccattt tatcagcgtt
     1381 atttgattta tgttgttttt ctttttcctt tggtgtcagg catcttcttc ccgattgcaa
     1441 cgcttgactc atccatagtc aaaacaaaag ggttctcgtt taaggcgatg gagagcggag
     1501 atcactactc ccaaacacag tatttgcggc ctgatgccag cctttattac gcgcaagaca
     1561 gctatgacaa acaaatgaaa cagctgttta ataagtattc cagcaaaaaa gagatttctc
     1621 tgaccgatga cgatttccta aaaggaatgg aaaccatcta taactatccg ggtgaatttc
     1681 tcggacggac gatagaattt catggatttg cttacaaagg aaacgcaatt aacaaaaatc
     1741 agttgttcgt attaaggttt ggcatcattc actgtatcgc tgattccggc gtgtacggga
     1801 tgcttgtgga atttccgaag gacatggaca tcaaggatga tgaatggatt cacattaagg
     1861 gcactctggc ctcagaatat taccagccat tcaagtcaac tctccctgtc gtcaaagtaa
     1921 ctgactggaa taccattaaa aaacctgatg atccgtatgt ttacagaggc tattaaacag
     1981 gtttgaggaa caaaattgtt cctcaaacct acttatatat tttttagcac tttaacccta
     2041 tacatattaa tttagttact tttgtcagtt aagttcgatc actcgactgc attgatttga
     2101 gacataagga tcatgagtta ctattattat tgttttattc atctgattta attcttttaa
     2161 aagggtgata atttcattgc gattctttac atctaaagag cctgttggtt catctgcaag
     2221 aataatatca catggtttta atatgattct tgctattgct aatctttgtt gttcgccccc
     2281 agaaagctca tggacttttt gtgttaagga cactttttgc aaacccactt tttcaagaac
     2341 cgcaatcttc aatttttcct tttcttctct ctttaacttt ttataagtta atgggacatc
     2401 taaattcgca ctgatactag cattatctat caaggcgtaa ttttgaaaca aatatgatat
     2461 tttagaacgc aacaattcat tggctcttcc agaaccaatt ctcggagcaa catccccaag
     2521 tattttaaca atcccaccat ctggcttttc gagcataccc atgatattaa gtattgttgt
     2581 ttttcccgaa ccactgctac ctgtaattgc aaccatttcc cctttttcta taacaatgtt
     2641 gacgttttcc aatacaacat gtttcccgta agcttttctt atattttgca attccataac
     2701 ataactcaat taatcagaaa cctcctttca taactttaat cttgtttctt cgttctataa
     2761 taattaatgc aattatcgaa gtaaccaatt ctaaaattaa aagaaataca atacaaatta
     2821 ataggtttaa agttattcct cttttgataa caatacaaga tataaattga ataagccata
     2881 gtataaaaaa caaggtcata taatctttaa atgttctaaa aaaaccaata ccaaacaatc
     2941 tatagactac aaacttttgt tgcttcttat tgaagtatat tacagaattt tgcacgataa
     3001 gaagaaccaa agcgacagct aatacagcag ctaaagctaa cagtgagcta atttcgctat
     3061 gtaactcttt tatttcattc aatgcatatt gatttaaaga gaccaagtac ttcaaattat
     3121 catcaagatg atattttttt aaataaggca ctaatatatt cattgtcttt tttgaatctc
     3181 tattatttaa ttttatttta agcggatctc gacttccatt tcctaaaatt gtatttctgt
     3241 cacctaccaa gctattattc tctgtaacaa cttggataat aggatctata attagattat
     3301 cctcagcttt aaaaacttct gggttaaaac taaatatttt ctgaccacta gcagtccaaa
     3361 taatatgtat tttaggttct tttaaatact ttggtacatg tctccctaaa aactcttcat
     3421 cataagctac tgcagattct tttctatctt ctttaaaaaa tttaattatt ttttctctat
     3481 gttgtttata tttctctgga acaagtaata ttaatctttt ttctctctca ttaattgata
     3541 ttggttgttt ttctgagttg tatacaggaa attgttttaa ataattatca tttacaacaa
     3601 tagacctgat tcccttatag ctagagttta atcgaataaa ttcctcttca tattctcttg
     3661 tatctattaa gagagcaccc attttgttca aaatggggta aatattatca atattatttg
     3721 taagttgtgg cttcccgtta ttaacatcct caatatcatg acctatatac aaaggattga
     3781 aaaccccata atctttacta accttccaat cttctaaatg agattgtttt tttttcactt
     3841 cactatattg atttaaagtt gaactaccta tcacaattat taagaccgaa caaactattt
     3901 ttaaaataga attaaaaata aagatgctat ttgtatcttt cttatttttt attgctagat
     3961 taacttttac ctttcttata taaaaataag taagtattga taatgagcct ataatcaaat
     4021 aattaataat ttgtttcatg atggtattca taataaaaga aaaggtggaa ccaggtaaga
     4081 aaattgcgca tatgattgat aataacaaag aaaaacaaaa cgttaatatt aataatttcc
     4141 ctactatttg aaaccaaatt cttaaattag aatatccatg taattttaaa acacctattg
     4201 tttttgactc attcacgata taataaataa gaagaaacaa tgttacaata agaatcaaat
     4261 agttgtactg ttgtaatgac gaggtagaga tttccgcata actatcatta acttctcgag
     4321 tttgtaaaaa gtcatttatt tcaaacgtcg aatcaaattt ttcatttatc tcattaacaa
     4381 atccctttaa aaaatcttca aaagaatact tacctttaat ttcagtataa tatgtaccgt
     4441 ttacaggaag ataatcaaat gagtttttta gtggtttaat tgttatttca gaagtgatac
     4501 cgaatttttc tatttgtcct atctgatttt tatcctttga tttatgcgta cttaagtaaa
     4561 aattttcaga ttgagtatcc aacttactta aaaatttccc ttgtgagagt tcaatgccat
     4621 taaaatagtc agtttgttgc gttaacaata catatttaga aatttccact ttatcgtttg
     4681 tagagaaatt agtagccggc cgaaaaatat tagttttata ttttttagaa ctactcataa
     4741 ttagttcgat catttcttta gggtcattta gtaagggatc atcaggaata gtaaaaggtt
     4801 ttccgacaga actatctagc tgttcaattt cttcaatttg tttatttgcg gtttccctaa
     4861 aggtaatcaa aaaggagaac agtgaagaaa tcactagaaa taaaatcaca atttttctca
     4921 caaaaaaaac tcctttataa aaaggagaat catcgaaaac taactctagt ttccgatgat
     4981 atccttatca aaattttata ttagtaagtt ttccaattgg cctttgtctt agcatctgtt
     5041 gcgccgcctg acaaatcgga gtatgaggtt gttcctgctt tagttttacc gcttgaatta
     5101 gttcttgtcc ctagagcaca tgatgaagca tgggccttat ctttgtgata ataatttgac
     5161 caaactttct ttttaaatcc ccaatcaaaa taaccagctg taccgtaatc ccagtttcct
     5221 ccgccaacat tttcattcgc aaatggggtt acttcagagt ttttcagagg cgtattagtt
     5281 aaatcaacct cgccacttgt tgctgcacct gctgctgatg ctgcttgtcc agttgataat
     5341 gctaatgctg ctattaaagc tgcactcatg agaccttttt tcaccataac taatccactc
     5401 cttcttttta aatgtagatt gaatgacagc gcgctcatcc gttcaacaac aacctgatta
     5461 taccatatat taacagtata gtaaataaat attttttcct ataaatggat cataaaagaa
     5521 agtgatctag atatattcta ttaacaaact cgcagaaaaa ctctctaaac aggctgtttt
     5581 atttcatcag catcaaatcg agatagagct ggcaccgcgt tctgaaatcg cttaaatcga
     5641 tcgacgtcat ctcagtaatc tgtttgatgc ggtaattcaa tgtattttgg tgaataaaca
     5701 gctgttctgc cgctggtttt gttttgcagt tgttgaggag atagatttcc aaggttttga
     5761 gcagctcagt gctgctttct tcgtctttcg cttttaataa agctaaatct ttattctcgt
     5821 attctaaaaa ttcatttttc tgttcgatgc tttctaaata acggtaaatg ccgagtttgg
     5881 catattcata tggaatgttt tcctgattgc cggtgatttc tgcggctttg atgacttcaa
     5941 gtgcttctgt gtagctcttg ccaagtttta ataggtgatt atattcgttt ccgataccga
     6001 tataaatagg ggtaggtatt ttttgaaagt gaaaatgtgt taacagcttg ttaataaatt
     6061 cagaagctgc tgaggagacg gagctttttt gggaaaaact cgctacgacg atgacaatgt
     6121 ttgattcgat cgttaagaca tgactgacct tatcccgtaa attcaggtac gacctgatat
     6181 tttcctttaa atcctcaacc gcttccccat ccccatttgc ggcgtgcagg atcacaactg
     6241 aaaacatgga aggcagcacg gtgttgatcc tttcagcttc ccgtctgagt tccttctcag
     6301 aggtaaattg ctgatagatc gcccgtttga tgagatcctc tgctttttct tccttttcct
     6361 gtttcaattt attcgtttta taaatgattt tcccgacatg gaaggaggtc tcgtacaaaa
     6421 agtcgagctc ttcatccgtc agattttggt ccagctcctg aatccagatg tagcccatga
     6481 cttccccttt gtgctttgca ctcacgacca ccctctggtt taacccgatg ctttcaattt
     6541 tattgacgcg aaaaggctca ggctctgttt tcaatttttc aataattccg tccttcagaa
     6601 acctctcaaa aatctggacc ggacactttt tcgaaaaaat agtctgctgg ttggcagaat
     6661 caaaatggtt aatatagtaa gaattatagg ctaacaaaaa gaaatccgcg ctttccagta
     6721 tcactggctt ttgcagttca tagctaataa aatcaattag cttatcaaca tctgaaaatg
     6781 aaaaaactct ctctaaaagc tcttccatac ctttttctcc tatacttttt ttactttaat
     6841 catatccata cggggtcttt ttgtatagaa aaaaatacgt ttgtttccaa aagaaaaaaa
     6901 tggccgctta aaaggagaaa agaggaaaaa gggacagccc cttcctcctc ctttcgatct
     6961 ttattgcgac attgtatctt ggtagtcgtt gaactggtct gtgacagctt gtgacggagc
     7021 ctgcgtcaat atgctgacaa aaaagacaga taatagactc gcagcaaaac caggtatcat
     7081 ttcatacaga aagtccgaaa gaccggcatt cgcccaaatg attacagtcg ctgctcccac
     7141 aatcatgccg gcgagtgccc cccatttggt cattcgtttc caaaacagac tgagcagcac
     7201 aaccggcccg aatgatgcac cgaagcccgc ccacgcgtag ctgaccaatc caaggatggt
     7261 gttatttttc tcccaagcca gaaccaacgc gacaattgaa acagccagaa cagccagtcg
     7321 gcctaaaaac accagttctt tatctgaagc tgagcgtctg aacatcgact tatacaaatc
     7381 ttctaccaga gagcttgatg tcacaagcag ctgggaagaa attgtactca taatcgcggc
     7441 caaaatcgct gaaatcagaa aacccgtgat aatcggatgg aacaagatat tccctaactg
     7501 aataaaaatc gtctctgggt ccttcagtgt catgccgcgc tgatggtaat aagcgattcc
     7561 gcccaaaccg gtcagcactg cgcctactgc agacaaaatc atccagccca tcccgattct
     7621 gcgcgctctt ttgatctctt tgacagacgt gatcgccata aaacgcacaa taatatgcgg
     7681 ctggccaaag tatcccagcc cccaggcaaa caaagaaata atgccaagga cgctcgttcc
     7741 tttaaaaatg ttaaacatat caggatctac agagcggatt tcagcgactg tatcacctgc
     7801 tcctcccgtg tggaaaaacg taacgatcgg cacaagaatg agtgcggcaa acataatgat
     7861 cccctgaaca aaatctgtcc agctgactgc caaaaagccg ccaaacaggg tatatgccac
     7921 aacaacacct gtcacaatcc acaggccggt gtgataatcc atccccaaga tgctgttgaa
     7981 taaaacgccg ccggacacca ttccggacga tacataaaac gtgaaaaaga ctaaaatgac
     8041 aattcctgaa aacagtctga gaagctttgt ttgatctccg aagcgatttt ccaggaatcc
     8101 gggaattgta atggaattcc ccgctttttc agtataggtt ctcagccgcg gtgcgacgta
     8161 aagccagttc gcccacgctc caaggcaaag tccgatgacg atccaagcgc cgctcagacc
     8221 agtagaaaac atggcgcccg gaagccccat tagcagccag ccgctcatat cggcagcgcc
     8281 ggcactgaga gcagttactg ccggacccaa agagcgtcct cccagcatgt aatccgtcag
     8341 attcgacgta cgcttgtaag caaagtagcc gattagcagc attcccgcca tataaataca
     8401 aatcgaaata atcagctgtg tgttttctat ggcacttcct cctctttttt atagtatatg
     8461 gatgaaatgg acacccccgc agggaggatg cccatttagt cccgcttaga acatttcact
     8521 gattgttttt gcttgcatat gcagagccaa gtaatccggc ccgcctgctt ttgaatccgt
     8581 tcccgacatt ttgaagccgc caaacggatg gtagccgacg atagcacctg tacagttgcg
     8641 gttgaagtat aggtttccga catggaattc ctgtttcgca cgctcgatgt gcttgcggtt
     8701 gtttgtgata accgcgcctg tcaaaccata ttcagtattg tttgccactt ctaaagcttc
     8761 atcaaagtct gacactttac aaaatgcaac gacaggtccg aaaatttctt cctgcatgag
     8821 tcttgctttc ggatcaaggt cagcgaagat cgtcggtttg atgaagtatc ctttcgaatc
     8881 atcaccagta ccgccgctta ctaaacgccc ttcctgtttt ccgatctcaa tatagctcat
     8941 aattttatca taagaacctt ggtcaatgac aggtcccata taaacatctg cactgtcagg
     9001 tttagctgtt acttttgatt ccgtaatttc aatgacacgc tctaatactt gatcatacac
     9061 tttttcatga acaactgcac gtgaacctgc agagcatttt tgtcccgcaa agccgaatgc
     9121 tgaagtaaag atcgattgag ccgctaattc aatgtccgca tcctcatcaa caacaaccgt
     9181 atctttaccg cccatttcag cgatgacacg ctttaaatgc tgctggcccg gctgaacctt
     9241 cgccgcgcgt tcgaaaattc tcgtaccaac ttctcttgat cccgtaaatg tgataaggct
     9301 tgttttcaga tggtcaacaa gatagtcgcc tacttccgct ccgcttcccg gaacaaagtt
     9361 gactacgcct tttggcaatc cggactcttc aagcacctca acaaattttg ctgcaataac
     9421 aggtgtagca ctcgcaggtt tcagaaccac tgtgtttcca gtaacgatcg gcgccactgt
     9481 tgtgcctgcc atgatcgcaa acaagaagtt ccaaggcggg ataacgactg tcactccagt
     9541 cggcgtgtat acatattggt ttttctcgcc ttcacggctg ttgaccggtt tgccttttgc
     9601 cagttcgatc atttggcgtg cataatactc catgaagtca atcgcttcag ccgtatcggc
     9661 atccgcctcg ttccaaggct ttcctgcttc tttcacaagc aaagctgaga attcatgttt
     9721 tcttctgcgg actttggcag cagcgcggaa caggacagcc gctctctctt caggagacgt
     9781 gtatctccac tcttcaaatg cttttgcagc cgcttgaatc gcttgctcag cgtgctcttg
     9841 agacgctttt gacactcggc cgacgacttc ttctttatca gccgggttga ttgaaacgat
     9901 tttcgcttcc gtttccactc tctcgccgtt aatgacaagc ggatagtctt ttcctaaata
     9961 ttcgcttact gtcgcaagcg cttttttaaa cgcttccacg ttgttttgat cttggaaatt
    10021 tgtgaatggc tcgtgtttgt aaggtgttgt catgataatc tctccttttt tacttctttg
    10081 tcattccttt gaaagcaaat gcaatgtttg acggacgttc tgcaaggcgt ctcataaagt
    10141 agccgtacca atcctcgccg tatggcaggt agactctcat gttataacct tcttttacga
    10201 ggctgagctg ggtttgcgac cgcatgccgt acagcatctg aaattcaaac ttgtcattgg
    10261 caatgccatg ttccttggca agctgctttg taaagtcgat cattttgtcg tcatgtgtgg
    10321 caatcgctgt atagtgaccg tttaagagct gctttcggat gatttttttg taattttcat
    10381 cgacatc
//
