LOCUS       NZ_NJRE01000016        10219 bp    DNA     linear   CON 22-JUN-2024
DEFINITION  Bacillus subtilis strain 18-3 18-3_R1_(paired)_contig_16, whole
            genome shotgun sequence.
ACCESSION   NZ_NJRE01000016
VERSION     NZ_NJRE01000016.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Cui,Y.
  TITLE     Improper usage of probiotics constitutes a potential risk of public
            health
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   Cui,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2017) Beijing Advanced Innovation Center for Food
            Nutrition and Human Health, College of Veterinary Medicine, China
            Agricultural University, Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 9.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010578165.1-RS_2024_06_22
            Annotation Date                   :: 06/22/2024 02:36:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,376
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5, 2 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 72 of 124
            Pseudo Genes (incomplete)         :: 64 of 124
            Pseudo Genes (internal stop)      :: 16 of 124
            Pseudo Genes (multiple problems)  :: 23 of 124
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:28:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10219
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NJRE01000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..10219
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>10217](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>10217)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..3180)
                     /gene="srfAB"
                     /locus_tag="CE047_RS22350"
                     /pseudo=""
     CDS             complement(<1..3180)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (12-415). E-value: 3.4e-116.
                     Score: 380.1. Matches aSDomain:
                     nrpspksdomains_CE047_RS22350_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (521-589). E-value: 1e-24. Score:
                     78.5. Matches aSDomain: nrpspksdomains_CE047_RS22350_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (605-892). E-value:
                     1.3e-106. Score: 347.9. Matches aSDomain:
                     nrpspksdomains_CE047_RS22350_Condensation_LCL.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="CE047_RS22350"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 1.4e-126, bitscore:
                     410.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.6e-17, bitscore:
                     50.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.1e-99, bitscore:
                     321.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQITEATFAALFEKQAQQTPDHPAVKAGGNLLTYRELDEQANQL
                     AHHLRAQGAGNEDIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSG
                     AKIAVVNERNMTAIGQYEGTIVSLDDGKWRNESKERPSSISGSRNLAYVIYTSGTTGKP
                     KGVQIEHRNLTNYVSWFSEEASLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQK
                     ETYTAPDEIAHYIKEHGITYIKLTPSLFHTMVNTASFAKDANFESLRLIVLGGEKIIPT
                     DVLAFRKMYGHTEFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLN
                     EALKLVPPGASGQLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSD
                     GTLAFIGRADDQVKIRGYRIEPKEIETVMLSLSGIQEAVVLAVSEGGLQELCAYYTSDQ
                     DIEKAELRYQLSLTLPSHMIPAFFVQVDAIPLTANGKTDRNALPKPNAAQSGGKALAAP
                     ETALEESLCRIWQKTVGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELEKSVPLKALFE
                     QPTVRQLAAYMEASAVSGGHQVLKPADKQAMYPLSSAQKRMYVLNQLDRQTISYNMPSV
                     LLMEGELDISRLRDSLNQLVNRHESLRTSFMEANGEPVQCILEEAEVDLHVFEAEEDEA
                     DQKIKEFIRPFDLNDAPLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYK
                     GEQLPEPALHYKDFAVWQNEAEQKERMKEHEAYWMSVLSGELPELDLPLDYARPPVQSF
                     KGDTIRFRTGSETAKAVEKLLAETGTTLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRT
                     NADVQDMPGMFVNTLALRMEAKEQQTFAELLEQAKQTNLSALEHQEYPFEDLVNQLDLP
                     RDMSRNPLFNVMVTTENPDKEQLTLQNLSISPYEAHQGTSKFDLTLGGFTDENGIGLQL
                     EYATDLFAKETAEKWSEYVLRLLKAVADNPNQPLSSLSLVTETEKQALLEAWKGKALPV
                     PTDKTVHQLFEE"
     source          1..10219
                     /collection_date="2016-07"
                     /db_xref="taxon:1423"
                     /geo_loc_name="China"
                     /mol_type="genomic DNA"
                     /note="commercial probiotic used for animal"
                     /organism="Bacillus subtilis"
                     /strain="18-3"
                     /submitter_seqid="18-3_R1_(paired)_contig_16"
     region          1..10219
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..10219
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(352..465)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22350_0009"
                     /evalue="6.10E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CE047_RS22350"
                     /protein_end="943"
                     /protein_start="905"
                     /score="57.2"
                     /tool="antismash"
                     /translation="KQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMV"
     aSDomain        complement(505..1365)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS22350_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.30E-106"
                     /label="CE047_RS22350_Condensation_LCL.1"
                     /locus_tag="CE047_RS22350"
                     /protein_end="892"
                     /protein_start="605"
                     /score="347.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="AMYPLSSAQKRMYVLNQLDRQTISYNMPSVLLMEGELDISRLRDS
                     LNQLVNRHESLRTSFMEANGEPVQCILEEAEVDLHVFEAEEDEADQKIKEFIRPFDLND
                     APLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPALHYKDFA
                     VWQNEAEQKERMKEHEAYWMSVLSGELPELDLPLDYARPPVQSFKGDTIRFRTGSETAK
                     AVEKLLAETGTTLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRTNADVQDMPGMFVNTL
                     ALRMEA"
     CDS_motif       complement(514..603)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22350_0008"
                     /evalue="1.10E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="CE047_RS22350"
                     /protein_end="889"
                     /protein_start="859"
                     /score="46.6"
                     /tool="antismash"
                     /translation="DIVIGSVTAGRTNADVQDMPGMFVNTLALR"
     CDS_motif       complement(862..894)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22350_0007"
                     /evalue="1.30E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="CE047_RS22350"
                     /protein_end="773"
                     /protein_start="762"
                     /score="14.6"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       complement(925..990)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22350_0006"
                     /evalue="1.90E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="CE047_RS22350"
                     /protein_end="752"
                     /protein_start="730"
                     /score="30.0"
                     /tool="antismash"
                     /translation="MHHIIADGVSRGIFVKELALLY"
     CDS_motif       complement(1186..1296)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22350_0005"
                     /evalue="6.60E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE047_RS22350"
                     /protein_end="665"
                     /protein_start="628"
                     /score="53.9"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGELDISRLRDSLNQLVNRHESLRTSF"
     aSDomain        complement(1414..1617)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS22350_PCP.1"
                     /evalue="1.00E-24"
                     /label="CE047_RS22350_PCP.1"
                     /locus_tag="CE047_RS22350"
                     /protein_end="589"
                     /protein_start="521"
                     /score="78.5"
                     /tool="antismash"
                     /translation="EESLCRIWQKTVGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELE
                     KSVPLKALFEQPTVRQLAAYMEA"
     aSModule        complement(1414..4419)
                     /complete
                     /domains="nrpspksdomains_CE047_RS22355_Condensation_LCL.1"
                     /domains="nrpspksdomains_CE047_RS22350_AMP-binding.1"
                     /domains="nrpspksdomains_CE047_RS22350_PCP.1"
                     /locus_tags="CE047_RS22350"
                     /locus_tags="CE047_RS22355"
                     /monomer_pairings="Asp -> Asp"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1906..1971)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22350_0004"
                     /evalue="3.60E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CE047_RS22350"
                     /protein_end="425"
                     /protein_start="403"
                     /score="38.1"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIEPKEIE"
     aSDomain        complement(1936..3144)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS22350_AMP-binding.1"
                     /evalue="3.40E-116"
                     /label="CE047_RS22350_AMP-binding.1"
                     /locus_tag="CE047_RS22350"
                     /protein_end="415"
                     /protein_start="12"
                     /score="380.1"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEKQAQQTPDHPAVKAGGNLLTYRELDEQANQLAHHLRAQGAGNE
                     DIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSGAKIAVVNERNMT
                     AIGQYEGTIVSLDDGKWRNESKERPSSISGSRNLAYVIYTSGTTGKPKGVQIEHRNLTN
                     YVSWFSEEASLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQKETYTAPDEIAHY
                     IKEHGITYIKLTPSLFHTMVNTASFAKDANFESLRLIVLGGEKIIPTDVLAFRKMYGHT
                     EFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLNEALKLVPPGASG
                     QLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSDGTLAFIGRADDQ
                     VKIR"
     CDS_motif       complement(2053..2142)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22350_0003"
                     /evalue="4.60E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="CE047_RS22350"
                     /protein_end="376"
                     /protein_start="346"
                     /score="50.9"
                     /tool="antismash"
                     /translation="PPGASGQLYITGQGLARGYLNRPQLTAERF"
     CDS_motif       complement(2671..2730)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22350_0002"
                     /evalue="1.00E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="CE047_RS22350"
                     /protein_end="170"
                     /protein_start="150"
                     /score="33.4"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVQIEH"
     CDS_motif       complement(2917..2958)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22350_0001"
                     /evalue="3.60E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="CE047_RS22350"
                     /protein_end="88"
                     /protein_start="74"
                     /score="17.0"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     gene            complement(<3253..>4425)
                     /locus_tag="CE047_RS22355"
                     /pseudo=""
     CDS             complement(<3253..4425)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (2-264). E-value:
                     6.2e-95. Score: 309.6. Matches aSDomain:
                     nrpspksdomains_CE047_RS22355_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="CE047_RS22355"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="condensation domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 2.2e-86, bitscore:
                     278.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMID
                     GEVVQTVHKNISFQLEAAKGREEDAEEIIKAFVQPFELNRAPLVRSKLVQLEEKRHLLL
                     IDMHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDV
                     FKGELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFN
                     VLLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTAPAGDKTFAQFLEEVKTA
                     SLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNMQNMEIPSLRLGDLKISSYSMLH
                     HVAKFDLSLEAVEREEDIGLSFDYATALFKDETIRRWSRHFVNIIKAAAAN"
     CDS_motif       complement(3361..3408)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22355_0006"
                     /evalue="1.00E+01"
                     /label="C3_dual_135-156"
                     /locus_tag="CE047_RS22355"
                     /protein_end="355"
                     /protein_start="339"
                     /score="-0.4"
                     /tool="antismash"
                     /translation="HHVAKFDLSLEAVERE"
     CDS_motif       complement(3472..3585)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22355_0005"
                     /evalue="7.60E-17"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CE047_RS22355"
                     /protein_end="318"
                     /protein_start="280"
                     /score="53.6"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNM"
     aSDomain        complement(3634..4419)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS22355_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="6.20E-95"
                     /label="CE047_RS22355_Condensation_LCL.1"
                     /locus_tag="CE047_RS22355"
                     /protein_end="264"
                     /protein_start="2"
                     /score="309.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="NTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMIDGE
                     VVQTVHKNISFQLEAAKGREEDAEEIIKAFVQPFELNRAPLVRSKLVQLEEKRHLLLID
                     MHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDVFK
                     GELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFNVL
                     LSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTA"
     CDS_motif       complement(3640..3729)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22355_0004"
                     /evalue="3.00E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="CE047_RS22355"
                     /protein_end="262"
                     /protein_start="232"
                     /score="51.7"
                     /tool="antismash"
                     /translation="DIIVGSPTAGRTHPDLQGVPGMFVNTVALR"
     CDS_motif       complement(3976..4011)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22355_0003"
                     /evalue="4.10E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="CE047_RS22355"
                     /protein_end="150"
                     /protein_start="138"
                     /score="13.1"
                     /tool="antismash"
                     /translation="HYKDYAVWHKEQ"
     CDS_motif       complement(4042..4107)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22355_0002"
                     /evalue="2.80E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="CE047_RS22355"
                     /protein_end="128"
                     /protein_start="106"
                     /score="29.5"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKIY"
     CDS_motif       complement(4303..4416)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22355_0001"
                     /evalue="5.20E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE047_RS22355"
                     /protein_end="41"
                     /protein_start="3"
                     /score="57.4"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSF"
     gene            complement(4371..4511)
                     /gene="comS"
                     /locus_tag="CE047_RS22360"
     CDS             complement(4371..4511)
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="CE047_RS22360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComS"
                     /protein_id="WP_010886404.1"
                     /transl_table=11
                     /translation="MNRSGKHLISSIILYPRPSGECISSISLDKQTQATTSPLYFCWRE
                     K"
     aSDomain        complement(<4555..4737)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS22365_PCP.1"
                     /evalue="8.40E-20"
                     /label="CE047_RS22365_PCP.1"
                     /locus_tag="CE047_RS22365"
                     /protein_end="1031"
                     /protein_start="970"
                     /score="62.8"
                     /tool="antismash"
                     /translation="EKKLAEIWEGILGVRAGVTDNFFMIGGHSLKAMMMTAKIQEHFHK
                     EVPIKVLFEKPTIQEL"
     aSModule        complement(<4555..7629)
                     /complete
                     /domains="nrpspksdomains_CE047_RS22365_Condensation_DCL.1"
                     /domains="nrpspksdomains_CE047_RS22365_AMP-binding.1"
                     /domains="nrpspksdomains_CE047_RS22365_PCP.1"
                     /locus_tags="CE047_RS22365"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     gene            complement(<4555..7647)
                     /gene="srfAB"
                     /locus_tag="CE047_RS22365"
                     /pseudo=""
     CDS             complement(<4555..7647)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 1.4e-14.
                     Score: 45.0. Matches aSDomain:
                     nrpspksdomains_CE047_RS22365_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-305). E-value:
                     2.6e-115. Score: 376.6. Matches aSDomain:
                     nrpspksdomains_CE047_RS22365_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-864). E-value:
                     7.2e-113. Score: 369.2. Matches aSDomain:
                     nrpspksdomains_CE047_RS22365_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1031). E-value: 8.4e-20. Score:
                     62.8. Matches aSDomain: nrpspksdomains_CE047_RS22365_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="CE047_RS22365"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 3.9e-92, bitscore:
                     296.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 9.3e-118, bitscore:
                     381.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.6e-15, bitscore:
                     43.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFD
                     LEIFEKSVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHI
                     ERYKQDVQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNY
                     EALRAGRTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDE
                     YVNKEYSFTWDETLVARIQQTANLHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGR
                     PSEINGIETMAGLFINTIPVRVKVEGDAAFADIFTAVQQHAVEAERYDYVPLYEIQKRS
                     ALDGNLLNHLVAFENYPLDQELENGSMEDRLGFSIKVKRAFEQTSFDFNLIVYPGKTWT
                     VKIKYNGAAFDSAFIERTAEHLTRMMEAAVDQPAAFVREYGLVGDEEQRQIVEVFNSTK
                     AELPEGMAVHQVFEEQAKRTPASTAVVYEGTELTYRELNAAANRLARKLVEQGLQKGET
                     AAIMNDRSVETVVGMLAVLKAGAAYVPLDPELPGDRLRFMAEDSSVRMVLTSISYTEQT
                     HQLQVPVLTLDIGFEEQKAADNLNLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILRLV
                     KNAGYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFLRE
                     QRISTMWLTSPLFNQLAAKDADMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYG
                     PTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYILDEQQCLQPIGAPGELCVGGIGVAR
                     GYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVRGFRIELG
                     EIETKLNMAEHVTEAAVIIRKNKADENEICAYFTADREVAVSELRKTLSQSLPDYMVPA
                     HLIQMDSLPLTPNGKINKKELPAAQSEAVQPEYAAPETESEKKLAEIWEGILGVRAGVT
                     DNFFMIGGHSLKAMMMTAKIQEHFHKEVPIKVLFEKPTIQEL"
     CDS_motif       complement(5026..5091)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22365_0010"
                     /evalue="1.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CE047_RS22365"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        complement(5056..6237)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS22365_AMP-binding.1"
                     /evalue="7.20E-113"
                     /label="CE047_RS22365_AMP-binding.1"
                     /locus_tag="CE047_RS22365"
                     /protein_end="864"
                     /protein_start="470"
                     /score="369.2"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQAKRTPASTAVVYEGTELTYRELNAAANRLARKLVEQGLQKG
                     ETAAIMNDRSVETVVGMLAVLKAGAAYVPLDPELPGDRLRFMAEDSSVRMVLTSISYTE
                     QTHQLQVPVLTLDIGFEEQKAADNLNLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILR
                     LVKNAGYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFL
                     REQRISTMWLTSPLFNQLAAKDADMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNG
                     YGPTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYILDEQQCLQPIGAPGELCVGGIGV
                     ARGYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       complement(5173..5262)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22365_0009"
                     /evalue="2.80E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="CE047_RS22365"
                     /protein_end="825"
                     /protein_start="795"
                     /score="48.4"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGIGVARGYVNLPELTEKQF"
     CDS_motif       complement(5764..5823)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22365_0008"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="CE047_RS22365"
                     /protein_end="628"
                     /protein_start="608"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       complement(6010..6051)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22365_0007"
                     /evalue="1.60E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="CE047_RS22365"
                     /protein_end="546"
                     /protein_start="532"
                     /score="21.1"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPLD"
     CDS_motif       complement(6577..6693)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22365_0006"
                     /evalue="1.70E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="CE047_RS22365"
                     /protein_end="357"
                     /protein_start="318"
                     /score="55.8"
                     /tool="antismash"
                     /translation="QQHAVEAERYDYVPLYEIQKRSALDGNLLNHLVAFENYP"
     aSDomain        complement(6733..7629)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS22365_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="2.60E-115"
                     /label="CE047_RS22365_Condensation_DCL.1"
                     /locus_tag="CE047_RS22365"
                     /protein_end="305"
                     /protein_start="6"
                     /score="376.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFDLEIFEK
                     SVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHIERYKQD
                     VQRQGFNLAKDILFKVAVFRLDADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAG
                     RTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDEYVNKEY
                     SFTWDETLVARIQQTANLHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGRPSEING
                     IETMAGLFINTIPVRVKV"
     CDS_motif       complement(6742..6837)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22365_0005"
                     /evalue="7.50E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="CE047_RS22365"
                     /protein_end="302"
                     /protein_start="270"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       complement(7075..7113)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22365_0004"
                     /evalue="1.20E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="CE047_RS22365"
                     /protein_end="191"
                     /protein_start="178"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       complement(7159..7224)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22365_0003"
                     /evalue="1.20E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="CE047_RS22365"
                     /protein_end="163"
                     /protein_start="141"
                     /score="37.0"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       complement(7441..7557)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22365_0002"
                     /evalue="2.80E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="CE047_RS22365"
                     /protein_end="69"
                     /protein_start="30"
                     /score="45.3"
                     /tool="antismash"
                     /translation="SYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFVH"
     aSDomain        complement(7561..7647)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS22365_NRPS-COM_Nterm.1"
                     /evalue="1.40E-14"
                     /label="CE047_RS22365_NRPS-COM_Nterm.1"
                     /locus_tag="CE047_RS22365"
                     /protein_end="29"
                     /protein_start="0"
                     /score="45.0"
                     /tool="antismash"
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHS"
     CDS_motif       complement(7582..7620)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS22365_0001"
                     /evalue="2.40E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="CE047_RS22365"
                     /protein_end="22"
                     /protein_start="9"
                     /score="16.9"
                     /tool="antismash"
                     /translation="YALTPMQEGMLYH"
     CDS             complement(7660..>10217)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (308-376). E-value: 2e-27. Score:
                     87.2. Matches aSDomain: nrpspksdomains_CE047_RS21945_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (386-689). E-value:
                     5e-178. Score: 585.3. Matches aSDomain:
                     nrpspksdomains_CE047_RS21945_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (694-848). E-value: 1.1e-55.
                     Score: 179.6. Matches aSDomain:
                     nrpspksdomains_CE047_RS21945_TIGR01720.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="CE047_RS21945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_163136934.1"
                     /sec_met_domain="AMP-binding (E-value: 4.8e-62, bitscore:
                     198.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.9e-18, bitscore:
                     55.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 4.7e-98, bitscore:
                     316.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="AKYAGFGFDASIWEMFPTWTIGAELHVIDEAIRLDIVRLNNYFET
                     NGVTITFLPTQLAEQFMELENTSLRVLLTGGDKLKRAVKKPYTLVNNYGPTENTVVATS
                     AEIHPEEGSLSIGRAIANTRVYILGEGNQVQPEGVAGELCVAGRGLARGYLNREDETAK
                     RFVADPFVPGERMYRTGDLVKWTGGGIEYIGRIDQQVKVRGYRIELSEIEVQLAQLSEV
                     QDAAVTAVKDKGGNTAIAAYVTPETADIEALKSALKETLPDYMIPAFWVTLNELPVTAN
                     GKVDRKALPEPDIEVGSGEYKAPTTDMEELLAGIWQDVLGMSEVGVTDNFFSLGGDSIK
                     GIQMASHLNQHGWKLEMKDLFQHPTIEALTQYVERAEGKQADQGPVEGEVILTPIQRWF
                     FEKNFTNKHHWNQSVMLHAKKGFDPERVEKTLQALIEHHDALRMVYREGQEDVIQYNRG
                     LEAASAQLEVIQIEGQAADYEDRIEREAERLQSSIDLQEGGLLKAGLFQAEDGDHLLLA
                     IHHLVVDGVSWRVLLEDFAAVYTQLEQGNEPVLPQKTHSFAEYAERLQDFANSKAFLKE
                     KEYWRQLEEQAVAAKLPKDRESGDQRMKHTKTIEFSLTAEETEQLTTKVHEAYHTEMND
                     ILLTAFGLAMKEWTGQDRVNVHLEGHGREEIIEDLTISRTVGWFTSMYPMVLDMKHADD
                     LGYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKEDVAFSIQPDVSFNYLGQFDEMSDA
                     GLFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSLAYHSLEFHEKTVQTFSDSFK
                     AHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKLMEIF"
     gene            complement(7660..>10219)
                     /locus_tag="CE047_RS21945"
     aSDomain        complement(7672..8133)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21945_TIGR01720.1"
                     /evalue="1.10E-55"
                     /label="CE047_RS21945_TIGR01720.1"
                     /locus_tag="CE047_RS21945"
                     /protein_end="848"
                     /protein_start="694"
                     /score="179.6"
                     /tool="antismash"
                     /translation="LGYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKEDVAFSIQPDV
                     SFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSLAYHSLE
                     FHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKL"
     aSModule        complement(7672..9291)
                     /domains="nrpspksdomains_CE047_RS21945_PCP.1"
                     /domains="nrpspksdomains_CE047_RS21945_Epimerization.1"
                     /domains="nrpspksdomains_CE047_RS21945_TIGR01720.1"
                     /incomplete
                     /locus_tags="CE047_RS21945"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(8050..8109)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21945_0008"
                     /evalue="1.60E-09"
                     /label="NRPS-E6"
                     /locus_tag="CE047_RS21945"
                     /protein_end="722"
                     /protein_start="702"
                     /score="30.0"
                     /tool="antismash"
                     /translation="KEDIRHVPNKGVGYGILRYL"
     aSDomain        complement(8149..9057)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21945_Epimerization.1"
                     /evalue="5.00E-178"
                     /label="CE047_RS21945_Epimerization.1"
                     /locus_tag="CE047_RS21945"
                     /protein_end="689"
                     /protein_start="386"
                     /score="585.3"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="EGEVILTPIQRWFFEKNFTNKHHWNQSVMLHAKKGFDPERVEKTL
                     QALIEHHDALRMVYREGQEDVIQYNRGLEAASAQLEVIQIEGQAADYEDRIEREAERLQ
                     SSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRVLLEDFAAVYTQLEQGNEPV
                     LPQKTHSFAEYAERLQDFANSKAFLKEKEYWRQLEEQAVAAKLPKDRESGDQRMKHTKT
                     IEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDRVNVHLEGHGREEII
                     EDLTISRTVGWFTSMYPMVLDM"
     CDS_motif       complement(8155..8202)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21945_0007"
                     /evalue="1.60E-08"
                     /label="NRPS-E5"
                     /locus_tag="CE047_RS21945"
                     /protein_end="687"
                     /protein_start="671"
                     /score="26.5"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMYPMVL"
     CDS_motif       complement(8611..8676)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21945_0006"
                     /evalue="9.40E-12"
                     /label="NRPS-E2"
                     /locus_tag="CE047_RS21945"
                     /protein_end="535"
                     /protein_start="513"
                     /score="37.0"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRVLLEDF"
     CDS_motif       complement(8878..8952)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21945_0005"
                     /evalue="3.20E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE047_RS21945"
                     /protein_end="446"
                     /protein_start="421"
                     /score="19.5"
                     /tool="antismash"
                     /translation="FDPERVEKTLQALIEHHDALRMVYR"
     CDS_motif       complement(9013..9042)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21945_0004"
                     /evalue="5.60E-05"
                     /label="NRPS-E1"
                     /locus_tag="CE047_RS21945"
                     /protein_end="401"
                     /protein_start="391"
                     /score="15.8"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
     aSDomain        complement(9088..9291)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21945_PCP.1"
                     /evalue="2.00E-27"
                     /label="CE047_RS21945_PCP.1"
                     /locus_tag="CE047_RS21945"
                     /protein_end="376"
                     /protein_start="308"
                     /score="87.2"
                     /tool="antismash"
                     /translation="EELLAGIWQDVLGMSEVGVTDNFFSLGGDSIKGIQMASHLNQHGW
                     KLEMKDLFQHPTIEALTQYVERA"
     CDS_motif       complement(9577..9642)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21945_0003"
                     /evalue="1.70E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="CE047_RS21945"
                     /protein_end="213"
                     /protein_start="191"
                     /score="36.0"
                     /tool="antismash"
                     /translation="YIGRIDQQVKVRGYRIELSEIE"
     CDS_motif       complement(9721..9810)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21945_0002"
                     /evalue="3.40E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="CE047_RS21945"
                     /protein_end="165"
                     /protein_start="135"
                     /score="51.3"
                     /tool="antismash"
                     /translation="PEGVAGELCVAGRGLARGYLNREDETAKRF"
     CDS_motif       complement(9913..9942)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21945_0001"
                     /evalue="4.80E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="CE047_RS21945"
                     /protein_end="101"
                     /protein_start="91"
                     /score="9.6"
                     /tool="antismash"
                     /translation="NYGPTENTVV"
ORIGIN
        1 ctcttcgaat agctgatgaa ccgttttgtc tgtcggcaca ggcagcgctt tgcccttcca
       61 tgcctcaaga agcgcttgct tttctgtctc tgtgaccagt gacaggctgg aaagcggctg
      121 gttcgggtta tccgcaacag cctttagtag ccgcagaacg tattcgctcc atttttcagc
      181 tgtttctttc gcgaacagat ctgtcgcata ttcgagctgc aagccaatgc cattttcatc
      241 agtaaatccg cccagtgtca gatcaaactt agaagttccc tgatgagcct cataaggtga
      301 aatgctcaga ttttgcaatg taagctgttc tttatcaggg ttttctgtcg tcaccatcac
      361 attaaacaat gggtttcggc tcatatcccg agggagatca agctgattaa ccagatcttc
      421 aaacggatac tcctgatgct caagcgctga aaggttcgtc tgctttgctt gctccaaaag
      481 ctccgcaaat gtttgctgtt ctttcgcttc cattctcagg gcaagtgtat tgacgaacat
      541 tcccggcatg tcctgaacat cagcattcgt ccggccggca gtaacggagc cgatgacgat
      601 atcccgctgt ccggaaattt tgcttaaaaa gacgtggaaa acagcatgga gcaccatgtg
      661 caaggtcgtt ccggtttccg caagcagttt ttctaccgcc tttgccgtct cacttcccgt
      721 acggaaacgg atcgtatctc ctttaaagct ttgcacaggc ggacgggcat aatcgagcgg
      781 gagatcaagc tctggcagct cgccagaaag aaccgacatc caatacgcct catgctcctt
      841 catccgttct ttttgctcag cttcattttg ccaaacggcg aaatctttat aatgaagcgc
      901 cggctccgga agctgttctc ctttgtaaag cagcgccaat tcttttacaa aaatgcctct
      961 tgagacgccg tctgcgatga tgtgatgcat atctaaaagc agcaaatgtt tttttgcttc
     1021 tattcgaagc aaagctgcgc gaatgagcgg tgcgtcgttt aagtcgaatg gccggataaa
     1081 ttccttaatc ttttggtccg cttcgtcttc ttcggcttca aacacatgaa gatcaacctc
     1141 cgcctcctca aggatgcact gaacaggctc accattggct tccataaatg atgtccgcaa
     1201 tgattcgtga cgattcacaa gctgattgag tgagtcgcgc aggcgcgaaa tatcaagctc
     1261 tccttccatt agaagaacag atggcatgtt gtagcttatc gtctggcggt caagctgatt
     1321 gagcacgtac atccgtttct gtgcggatga caatggatac atagcctgct tgtcagccgg
     1381 tttgaggact tgatggccgc ctgaaacggc agacgcctcc atataagccg ccagctggcg
     1441 aacagtcggc tgctcgaaca gtgctttaag cggtacgctt ttctccaatt ccgcctgaat
     1501 gttggcaatc agcatcatcc cttttaatga atggccgcct aaatcgaaaa aattgtcatc
     1561 aatgccgatg gcttctatgc caactgtttt ctgccaaatg cggcataagc tttcttcgag
     1621 cgctgtctcc ggtgcggcca aggctttgcc tccggattgt gccgcgttcg gcttcggcaa
     1681 agcgtttctg tcggtttttc cgtttgccgt cagcggaatc gcgtcaacct gcacaaagaa
     1741 agcaggaatc atatgagacg gcagtgttag ggaaagctgg taccggagct ctgctttttc
     1801 aatatcttga tccgacgtat aatacgcgca aagctcttga agaccgccct cggaaaccgc
     1861 tagtacaacc gcttcttgaa ttccgctgag gctgagcatg accgtttcaa tttctttcgg
     1921 ctcaatgcgg tagcctcgga ttttcacctg atcatcagcc cggccgataa atgcaagcgt
     1981 accgtcagaa agtcttcgta cgacatctcc ggttttgtac atgaggcttc ccggcgaata
     2041 tggattttct acaaatctct cggctgtcag ctgaggcctg ttgagatacc ctctcgcaag
     2101 cccctgtccc gtgatataga gctgtccgct cgctcccggc ggcacaagct tcaatgcttc
     2161 atttaagaca agcgcgccgg cattcgcaat cgggcgtccg attgtcgggc gtttcgcaaa
     2221 tgcatccggc tcagacagat caacccgccc ggcgatggcg ccgatcgttg cttctgtcgg
     2281 gccgtagtga ttgataaatt cggtatgtcc atacatctta cggaaggcga gaacatcagt
     2341 cgggatgatt ttttctcctc ccaagacgat caagcgcaag gattcaaagt tcgcatcttt
     2401 tgcaaaactg gcggtgttca ccattgtatg gaacagagac ggtgtcagct tgatatatgt
     2461 gatcccatgc tccttgatat agtgcgctat ttcatccggc gccgtatagg tttccttctg
     2521 gacgatatgg agctcgcccc cgcccagaag tacagggaac atgctcgtat agccaaggtc
     2581 aaatgcgtaa gatgaaagca atacagtctt atcattttcc gtcaggctcg cctcttcact
     2641 aaaccaagag acatagtttg tcagattacg atgctcaatc tgcacgccct ttggctttcc
     2701 ggtcgtaccg gacgtataaa tgacgtatgc aagattgcga gacccggaaa tggatgatgg
     2761 gcgctccttg ctttcatttc tccatttacc gtcatcaagg ctgacgattg tcccttcata
     2821 ttgcccaata gccgtcatgt ttctttcatt cacgaccgca atttttgctc cgctgtcctc
     2881 taatgaataa cggattcgtt cttcaggtgt atcaggatca atcggaagga aagctgcccc
     2941 cgccttcatg acaccgagaa tcgataccat gacttcagct gaccggtcca taacaatcgc
     3001 gacaatgtct tcatttccgg ccccttgggc acgaagatga tgcgccagct ggttcgcctg
     3061 ttcatcaagc tcgcgatagg tcagcagatt tccgccagcc ttcaccgcag gatggtcagg
     3121 tgtttgctgg gcctgttttt caaaaagagc ggcaaaggtt gcttcggtaa tttgagtcat
     3181 atgtctttct tctagcaaag cagccgtttc tgcagatgaa agcagatcta catcagacag
     3241 ccgaacgttc ggattagccg cggccgcttt gatgatattg acaaagtggc ggctccagcg
     3301 gcggatcgtc tcgtccttaa acaaggcagt cgcatagtca aagcttaggc cgatatcctc
     3361 ttcacgctcg accgcttcca aggaaagatc aaatttcgca acatgatgaa gcatggaata
     3421 cgaggaaatc ttcaaatctc ctaatcttaa tgaaggaatc tccatattct gcatgttgaa
     3481 catcacgctg aacagcggac ttctgcttgt atcccttgta agcggaagct tttcaatcaa
     3541 ctcctcaagc ggatagctct ggtgctcgaa tgcttgaagg ctggctgttt tgacctcttc
     3601 aagaaattgc gcgaaggttt tatctcccgc tggtgccgtt ctgagtgcca ccgtgttgac
     3661 aaacataccc ggcacacctt gcagatcagg atgtgttctg ccagctgtcg gcgagccaac
     3721 aatgatatca tcctgtgacg cgtacttgga aaggagtaca ttgaacgccg ccagcaaaaa
     3781 catgtacatt gtcgtatctg tttctgccat gagcgatttg atttgagccg tgatttgctt
     3841 atcaagccca aacatcacgc gctctcccgc aaagctccgt tcagctggcc gctcgaaatc
     3901 cgcgggaaga tccagtattg gcagttcgcc tttaaagaca tcgagccagt attcctcatc
     3961 tttttgataa ttagtttgtt ctttgtgcca aactgcgtaa tctttatagt gaatttgtgg
     4021 cagttccaga tctgcgcctt gatatatttt ggcaagatca ccgattagaa tgcctgttga
     4081 acttccgtca gtaataatat gatgcatatc aatgagcagc aggtggcgtt tttcttccag
     4141 ctggacaagc ttcgaacgga cgagcggcgc gcggtttaat tcaaacggct gaacaaatgc
     4201 tttgattatc tcttccgcgt cttcttcccg tcccttggca gcctccagct ggaacgatat
     4261 gtttttatga acggtttgca caacctctcc gtcgatcatg tcaaacgatg tacggaggat
     4321 ttcgtgccgg ttgattaatt gctgaatcgc gttttcaagc cggtctttat ctacttctcc
     4381 ctccagcaga agtacagcgg ggacgttgta gcttgtgttt gcttgtccaa gctgattgag
     4441 gatatacatt ctccgctggg ccggggatac aggataatgc tgctgataag atgcttgcct
     4501 gatcggttca aacgtctgct cctccttgct ttcgttctct tccaaataca gtgccagttc
     4561 ttgaatagtc ggcttttcaa aaagcacttt tatcggaact tccttatgaa aatgctcttg
     4621 aattttcgcc gtcatcatca tcgctttcaa agaatggccg ccgatcataa agaagttatc
     4681 ggtaacgcct gctctgacgc cgagtattcc ttcccagatc tccgctaatt tcttttcact
     4741 ctctgtttct ggtgctgcgt attccggctg cacggcttct gattgcgctg caggcagttc
     4801 ttttttgttg attttcccgt ttggcgtgag cggcagactg tccatttgaa tcaggtgggc
     4861 agggaccata tagtcaggca aagactgaga cagtgttttt ctcagctcgc tcacagccac
     4921 ttcacggtcc gccgtaaagt acgcgcaaat ttcattttca tccgctttgt tcttgcggat
     4981 gatcaccgca gcctctgtca catgctcagc catgttcagt tttgtttcaa tttcgccaag
     5041 ctcaattcgg aagccgcgca ccttcacttg gttgtcgatt ctgcctaaaa attcgatatt
     5101 gccgtccggc agccatcttg ccaagtcacc agtgcgataa attctctcac ccggtctgaa
     5161 cggatcttcg agaaattgct tctctgtcag ctcagggaga ttgacatacc cacgcgctac
     5221 accgattccg cctacgcaaa gctcaccagg cgcgccgatt ggctgcaggc attgctgctc
     5281 atccaggatg taggcagtgg aattaccgat cggcttcccg attgggatag agccgccata
     5341 ctcgcggtcg atcagaaaac tggttgaaaa tgtcgtgttt tctgtcgggc cgtatccgtt
     5401 ccacaatgaa agcgacggcg atgcctgttt tactttgctg acaatatgcg ggacaagggc
     5461 atctccgccg atgattaaat ggcgcagtgt gccgaacata tccgcatcct ttgctgcaag
     5521 ctggttgaat aaaggtgatg tcagccacat cgtcgagatt ctttgctcac gcaggaatgc
     5581 agcaaattgt tttgcatcaa gcagcgtttc ttttttgact ggataaagcg ctgcgccatt
     5641 cagcagtgcg ccgaatacct caaacgttcc ggcatcaaag ctgacagctc ccgtttgcgc
     5701 catgcggtcc tcttcagtaa caggaacgta ccctgcattt ttgacgaggc gcagaatgct
     5761 tttatgttcg atcatgacgc ctttcggttt gcccgttgaa ccggatgtgt acatgatgta
     5821 tgccaaatca gacggggcgg atggcaggtt gagattgtca gccgcttttt gctcttcaaa
     5881 acctatgtcc agtgtaagaa ccggcacttg cagctgatgc gtctgctctg tataagaaat
     5941 actggtcaag accattcgaa cggagctgtc ctctgccatg aaacgaagac gatcccccgg
     6001 aagctcagga tcaagcggca catatgcggc acctgctttt aacacagcca gcatgccgac
     6061 aacggtttct actgagcgat cgttcataat cgctgctgtt tccccttttt gaaggccttg
     6121 ttcgacaagc tttctcgcca gacggttagc cgctgcattc agctcgcggt acgtcagctc
     6181 ggttccttca tatacgacgg cagtgctcgc cggcgtccgt tttgcttgct cttcaaacac
     6241 ttggtgaaca gccattcctt cagggagttc ggctttcgtg ctgttaaaca cctcgacaat
     6301 ttgccgctgc tcttcgtctc ccacaagccc gtactcacgc acaaaagcgg ccggctgatc
     6361 gacagctgct tccatcatgc gggtaaggtg ctccgctgtc cgttcgataa aagcggaatc
     6421 aaaggcggct ccgttatatt taattttgac ggtccacgtt ttgcccggat acacaatcag
     6481 gttgaaatcg aagctcgttt gttcaaatgc tctttttacc ttaatcgaaa acccgaggcg
     6541 gtcttccatg ctgccgtttt caagctcttg atcaagcgga taattttcaa acgcgaccag
     6601 atggtttaag agattgccat caagagctga gcgtttttga atctcataga gcggcacgta
     6661 atcgtaacgc tctgcctcta ctgcatgctg ctgaacagct gtgaaaatat cagcgaatgc
     6721 agcatctcct tcaactttca cccgcactgg aatggtgttg ataaacagcc ccgccatcgt
     6781 ttcgattccg ttgatttcag acggtcgtcc cgagacgacc gttccgaaga ccacatcatc
     6841 cgtaaagttg tatttgctga gaacaatccc ccaaacggcc tgaaataggt taggccctgt
     6901 cacttgatgg agatttgcgg tttgctgaat acgggcaacc agtgtttcgt cccatgtaaa
     6961 tgaatattct ttattgacgt attcgtcttt tttcacaggc aggcggcccg ggagcacgct
     7021 tggctgttca aaacccgcca agcgctcgct ccagtagctc tccgcttctt cattgtcctg
     7081 ttttccaagc catttaatgt aatcggagta aggcttgccc tgaccgtttg caggtgtcct
     7141 tcctgctctg agcgcttcat agttttggaa caggcttttc atgaggacgc ccatgctcca
     7201 gccgtccatc ataatatgat gattgctcca gacaagatac agctgatctg catcaaggcg
     7261 gaaaaccgct accttgaaca atatgtcttt tgccaggtta aagccttggc gctgaacgtc
     7321 ttgtttgtaa cgctcaatat gctccttctg gcggttctcg tctgcatgac tgatatcctc
     7381 ataatgcacc tttgttttgc gttccgctaa cacgacctga cgcggttttt gcagctgctg
     7441 atgcacaaat accgtacgga gaatatcgta tgaccgaatc agttcattga cgcttttctc
     7501 aaagatttca agatcaaaag cgccgtgaat cccaagctct aattgtgtga agtacgagga
     7561 agaatgcgga tcaagcatcg catgatacag cattccctcc tgcattggtg tcagtgcgta
     7621 caccttttga atcgattttt tgctcatatg ccacctctat tagaaaattt ccattaattt
     7681 atccagctca tccagcgtca aatcgtcgtc accaagatca ctcggggtca gttccgtacc
     7741 gtcttgagat aggcaatgtt caatgattct gagaagatgc gctttaaagc tgtcactgaa
     7801 tgtttgtact gttttttcat gaaattcaag agaatgatag gccagtgaca tcgtcagttt
     7861 tccgttttcg atatatccga caacatccag cgcattcggt ttttctgttt cagggcttaa
     7921 tgactgtcct gatggcagct ctgatctcgt aaacaaacct gcatccgaca tttcgtcaaa
     7981 ctgacctaaa taattgaagc tgacatccgg ctgaatcgag aacgccacat cttctttatg
     8041 ttccggtgcc gtcagatagc gcagaatgcc gtatccgact cccttattcg gcacatgtct
     8101 gatatcttct ttcatttgct tcagctggta gcccagatca tccgcatgct tcatatcgag
     8161 caccattggg tacatacttg taaaccagcc gactgtgcgg gaaatggtca ggtcttctat
     8221 gatttcttca cgtccgtgcc cctctaaatg aacatttact cgatcctgac ccgtccactc
     8281 cttcattgcc aatccgaatg ccgtcagcaa aatatcattc atttctgtgt gatatgcctc
     8341 atgcaccttt gtggtgagct gttctgtctc ttcagcagtc agcgagaatt caattgtctt
     8401 tgtatgtttc attcgctgat caccagattc gcggtctttc ggaagctttg ccgcgactgc
     8461 ttgttcttca agctgtctcc aatactcttt ttctttcaaa aaggctttgg agttcgcgaa
     8521 gtcttgcaat ctctctgcat attctgcaaa tgaatgtgtt ttctgcggga gaaccggttc
     8581 attgccttgc tcaagctgtg tatataccgc ggcgaaatcc tccagtaaaa cccgccacga
     8641 cacgccgtca accactaagt ggtgaatggc aagaagcaag tggtctccgt cttccgcttg
     8701 gaacaagcct gcttttaaca agccgccttc ctgcaagtcg atgctgcttt gtaaacgctc
     8761 cgcttctctc tctatacggt cttcgtaatc tgcagcttgg ccctcaatct ggatgacctc
     8821 caattgagct gaagcagctt caagacctct gttgtattga atgacgtctt cctgtccctc
     8881 acggtagacc atacggagcg cgtcatgatg ctcgatcagc gcctgcaatg ttttctccac
     8941 ccgttcagga tcaaatccct tcttggcgtg aagcatcacc gattggttcc agtggtgctt
     9001 gttcgtgaag ttcttttcaa aaaaccagcg ctggatcggc gtcaggatga cttcgccctc
     9061 caccgggcct tggtctgcct gtttgccttc ggcacgctct acatattggg taagcgcctc
     9121 gatcgtcggg tgctggaaga gatctttcat ttccagcttc cagccgtgct ggttcaagtg
     9181 gctcgccatt tgaattcctt tgatggaatc tccgccaagc gagaagaaat tgtcggtgac
     9241 accgacttca gacattccaa gcacgtcctg ccagatgccg gcaagcagct cttccatgtc
     9301 ggtcgtcggc gctttgtatt ctccgcttcc cacttcgatg tccggctcag gcaaggcttt
     9361 gcggtccact ttgccgtttg ccgtaaccgg aagctcgttc agcgtcaccc agaacgccgg
     9421 gatcatgtaa tccggcaggg tttccttcag tgctgatttc agtgcttcta tgtcagctgt
     9481 ttccggtgtg acatacgccg cgatcgctgt gttgccgcct ttatctttga cagccgtcac
     9541 tgccgcatcc tgtacctcag aaagctgggc gagctggact tcaatttctg agagctcgat
     9601 ccggtagccg cgaaccttga cctgctggtc gatccggccg atgtattcga tgccgccgcc
     9661 cgtccatttc accaaatcgc cggtgcggta catgcgctca cccggcacaa acggatcagc
     9721 gacaaaccgc ttcgcggttt cgtcttctcg attcagatag cctcgtgcca gtccgcgccc
     9781 cgccacgcaa agctctccgg ctacgccttc cggctgcacc tgattgccct cgccgaggat
     9841 gtatactctc gtattggcga tcgcccgtcc gatggaaagc gagccttcct ccggatggat
     9901 ttctgcgctt gtggcaacga ccgtgttttc tgtcggtccg tagttgttga cgagtgtgta
     9961 cggctttttc actgcgcgct tcagcttgtc tcctccggtc aagaggacgc gaagtgatgt
    10021 gttctcaagc tccatgaact gttccgcaag ctgtgtcggc aggaacgtga tcgttacgcc
    10081 gttcgtttcg aagtaattgt tcaggcggac gatatcgagg cggatcgctt catcaatgac
    10141 gtgaagctca gcgccgatcg tccaagtcgg gaacatctcc caaatggaag cgtcgaatcc
    10201 aaagcccgcg tacttcgca
//
