LOCUS       NZ_NJRE01000015         9426 bp    DNA     linear   CON 22-JUN-2024
DEFINITION  Bacillus subtilis strain 18-3 18-3_R1_(paired)_contig_15, whole
            genome shotgun sequence.
ACCESSION   NZ_NJRE01000015
VERSION     NZ_NJRE01000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Cui,Y.
  TITLE     Improper usage of probiotics constitutes a potential risk of public
            health
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Cui,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2017) Beijing Advanced Innovation Center for Food
            Nutrition and Human Health, College of Veterinary Medicine, China
            Agricultural University, Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 9.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010578165.1-RS_2024_06_22
            Annotation Date                   :: 06/22/2024 02:36:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,453
            CDSs (total)                      :: 4,376
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5, 2 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 72 of 124
            Pseudo Genes (incomplete)         :: 64 of 124
            Pseudo Genes (internal stop)      :: 16 of 124
            Pseudo Genes (multiple problems)  :: 23 of 124
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:28:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9426
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NJRE01000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9426
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>9426](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>9426)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..5868)
                     /gene="ppsD"
                     /locus_tag="CE047_RS21930"
                     /pseudo=""
     CDS             complement(<1..5868)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-30). E-value: 1.9e-18.
                     Score: 57.4. Matches aSDomain:
                     nrpspksdomains_CE047_RS21930_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (7-304). E-value:
                     1.1e-117. Score: 384.4. Matches aSDomain:
                     nrpspksdomains_CE047_RS21930_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (468-865). E-value:
                     3.5e-104. Score: 340.6. Matches aSDomain:
                     nrpspksdomains_CE047_RS21930_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (971-1040). E-value: 4.5e-24. Score:
                     76.5. Matches aSDomain: nrpspksdomains_CE047_RS21930_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1054-1333). E-value:
                     4e-103. Score: 336.4. Matches aSDomain:
                     nrpspksdomains_CE047_RS21930_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1499-1900). E-value:
                     1.8e-113. Score: 371.2. Matches aSDomain:
                     nrpspksdomains_CE047_RS21930_AMP-binding.2"
                     /codon_start=1
                     /gene="ppsD"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389713.2"
                     /locus_tag="CE047_RS21930"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="plipastatin non-ribosomal peptide synthetase
                     PpsD"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 6.9e-89, bitscore:
                     284.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.9e-15, bitscore:
                     43.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.9e-123, bitscore:
                     398.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLQKDSQAYVEQASFTIEGKV
                     NPQFFQNSINALVERHDIFRTIFISQNVSSPQQVVLRERNVIVLEENITHLNEAEQSQF
                     IEQWKEKDRDRGFHLQKDVLMRIALIQTGESQYSCIWTFHHIMMDGWCLSIVLKEFLHI
                     YASYVNASPITLEPVQPYGKYIKWLMEQDKEQAVSYWDHYLSGHEQQTVLPKQKKTKGK
                     SRQEHVTFSFSKEESSRLSELAAREEVTLSTIFHTIWGILLQKYNNNDDAVFGSVISGR
                     PAEIEGIEHMVGLFINTMPVRVQGAKTPFLQLIKDMQKDRLAAEAYSYHPLYEIQSRSA
                     VKQGLIDHILVFENYPVQQEIQMLNKQEHATDLFQIHNFTVADETNYSFYLMVAPGEEI
                     HIKMNYDAEQHDRSFVLSVKEHLLNAASQILNNPNLPPEEIDITTDTEKRQLIGEITDR
                     TPVYETIHAMFEKQAEKTPDAHAVIDQACSLTYRELNKAANRLARHLRMKGVVRQEPVA
                     IMMDRSAAFISGVLGILKAGGAIVPVDPHYPADRIRYILHDCGCSHVVSQAHLSSSLED
                     NYIITHPEEIESKVDGSNIKSVNDADDLLYMIYTSGSTGKPKGVQFEHRNMANLLKFEY
                     THSGIDFEADVLQFATPSFDVCYQEIFSALLKGGTLHIVPEDIKRDVPQLFTFINKHQT
                     NIVFLPTAFIKMIFSERELANSFPDGVKHLIAAGEQLMISDLFQNVLRERGIHLHNHYG
                     PSETHVVSTYTIHPGDPIPELPPIGKPIGCTDLYILNHQKQLQPCGVPGELYISGASVA
                     RGYVNHDKLTSDKFSSDPFRPGVIMYRTGDLARRLEDGNIEYIGRADNQVKIRGYRIEP
                     QEIEVTLMNHPDISEAAILIWQDQNGEHELCAYYCSVQKLNAIDLRSYMASELPEYMIP
                     AKWVWVDSIPLTPNGKVDRAALPEPDASISGNPYTAPRNLLEAKLSQLFEDVLKNGHVG
                     IQDNFFDNGGHSLKATVLMSRIAKEFHVQVSLKDIFAHPTVEGLALIIREAEQNPYAAI
                     EPAEKRDTYPVSSAQKRIYVLQQLDEGVAYNMPAVLELEGALDVAKLSAVCKELISRHE
                     PLRTSFVSGADDEPVQRIHTEVPFTLSKETTIEGFVRPFDLSQAPLFRAGLIEVSNEKH
                     VLLVDMHHIISDGVSVQLLIREFTDLYANRQLKPLRIQYKDYAVWQQKFKKGDSYQKQE
                     TYWQQQFSGDLPILELPTDKRRPAERQFIGGKVTFQLDKEITARIKRLAHKNRNTLYMT
                     LLALYSAFLSRLSGQDDIVIGSPIAGRPHADLEAVLGMFVNTLALRTRPAGNKTFEEFL
                     KEVRQTALEAYEHQDYPFEELVDKLGVQREMSRNPLFDTTLVLQNMEQQKLKMNDVQLQ
                     WNDLVHPISKFDISLYVTEHDSELFCQFEYSTALFEKETIQRWASLFTTLVEHTAASPE
                     TELDNIPILTKEEERDFIESCHLFEETGYSMNQTLHYALEQQAAKTPDQTAVVFEDGAM
                     TYKELNEQANRIAWELIGRGVKSETTVAIMGRRSPEMLIGIFGILKAGGAYLPIDPDYP
                     EERISFLLEDSGTNILLLQSDGLHVPAFAGEIVYLNQTNSGLKHRLSNPNVDVLPQSLA
                     YVIYTSGSTGRPKGVEVEHRSAVNFLNSLQSRYQLNQSDVIMHKTSYSFDASIWELFWW
                     SYAGASVYLLPQGGEKEPEVIAQAIEEQKITAMHFVPSMLHVFLEHIKYRSVPIKTNRL
                     KRVFSGGEQLGTHLVSRFYELLPNVSITNSYGPTEATVEAAFFDCPPQEKLERIPIGKP
                     VHHVRLYILNQNQRMLPSGCIGELYIAGAGVARGYLNRPALTEEHFLEDPFYPGERMYK
                     TGDVARWLPDGNVEFLGRLDDQVKIRGYRVEPGEIEAALRSIEGVREAAVTVRTDSGEP
                     ELCAYVEGLQKNEVRAQLERLLP"
     source          1..9426
                     /collection_date="2016-07"
                     /db_xref="taxon:1423"
                     /geo_loc_name="China"
                     /mol_type="genomic DNA"
                     /note="commercial probiotic used for animal"
                     /organism="Bacillus subtilis"
                     /strain="18-3"
                     /submitter_seqid="18-3_R1_(paired)_contig_15"
     region          1..9426
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9426
                     /SMILES="NC(C(C)C)C(=O)N1C(CCC1)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(139..204)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0021"
                     /evalue="1.70E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1910"
                     /protein_start="1888"
                     /score="39.2"
                     /tool="antismash"
                     /translation="FLGRLDDQVKIRGYRVEPGEIE"
     aSDomain        complement(169..1371)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21930_AMP-binding.2"
                     /evalue="1.80E-113"
                     /label="CE047_RS21930_AMP-binding.2"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1900"
                     /protein_start="1499"
                     /score="371.2"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="LEQQAAKTPDQTAVVFEDGAMTYKELNEQANRIAWELIGRGVKSE
                     TTVAIMGRRSPEMLIGIFGILKAGGAYLPIDPDYPEERISFLLEDSGTNILLLQSDGLH
                     VPAFAGEIVYLNQTNSGLKHRLSNPNVDVLPQSLAYVIYTSGSTGRPKGVEVEHRSAVN
                     FLNSLQSRYQLNQSDVIMHKTSYSFDASIWELFWWSYAGASVYLLPQGGEKEPEVIAQA
                     IEEQKITAMHFVPSMLHVFLEHIKYRSVPIKTNRLKRVFSGGEQLGTHLVSRFYELLPN
                     VSITNSYGPTEATVEAAFFDCPPQEKLERIPIGKPVHHVRLYILNQNQRMLPSGCIGEL
                     YIAGAGVARGYLNRPALTEEHFLEDPFYPGERMYKTGDVARWLPDGNVEFLGRLDDQVK
                     IR"
     aSModule        complement(169..2706)
                     /domains="nrpspksdomains_CE047_RS21930_Condensation_LCL.1"
                     /domains="nrpspksdomains_CE047_RS21930_AMP-binding.2"
                     /incomplete
                     /locus_tags="CE047_RS21930"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(286..375)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0020"
                     /evalue="1.30E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1861"
                     /protein_start="1831"
                     /score="52.6"
                     /tool="antismash"
                     /translation="PSGCIGELYIAGAGVARGYLNRPALTEEHF"
     CDS_motif       complement(484..513)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0019"
                     /evalue="4.10E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1795"
                     /protein_start="1785"
                     /score="7.0"
                     /tool="antismash"
                     /translation="SYGPTEATVE"
     CDS_motif       complement(898..957)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0018"
                     /evalue="2.80E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1657"
                     /protein_start="1637"
                     /score="32.0"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVEVEH"
     CDS_motif       complement(1144..1185)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0017"
                     /evalue="5.90E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1575"
                     /protein_start="1561"
                     /score="16.3"
                     /tool="antismash"
                     /translation="FGILKAGGAYLPID"
     CDS_motif       complement(1711..1830)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0016"
                     /evalue="6.40E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1386"
                     /protein_start="1346"
                     /score="66.8"
                     /tool="antismash"
                     /translation="RQTALEAYEHQDYPFEELVDKLGVQREMSRNPLFDTTLVL"
     aSDomain        complement(1870..2706)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21930_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.00E-103"
                     /label="CE047_RS21930_Condensation_LCL.1"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1333"
                     /protein_start="1054"
                     /score="336.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="DTYPVSSAQKRIYVLQQLDEGVAYNMPAVLELEGALDVAKLSAVC
                     KELISRHEPLRTSFVSGADDEPVQRIHTEVPFTLSKETTIEGFVRPFDLSQAPLFRAGL
                     IEVSNEKHVLLVDMHHIISDGVSVQLLIREFTDLYANRQLKPLRIQYKDYAVWQQKFKK
                     GDSYQKQETYWQQQFSGDLPILELPTDKRRPAERQFIGGKVTFQLDKEITARIKRLAHK
                     NRNTLYMTLLALYSAFLSRLSGQDDIVIGSPIAGRPHADLEAVLGMFVNTLALRTRP"
     CDS_motif       complement(1879..1968)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0015"
                     /evalue="2.60E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1330"
                     /protein_start="1300"
                     /score="51.9"
                     /tool="antismash"
                     /translation="DIVIGSPIAGRPHADLEAVLGMFVNTLALR"
     CDS_motif       complement(2227..2259)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0014"
                     /evalue="3.30E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1214"
                     /protein_start="1203"
                     /score="13.4"
                     /tool="antismash"
                     /translation="QYKDYAVWQQK"
     CDS_motif       complement(2290..2355)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0013"
                     /evalue="1.80E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1193"
                     /protein_start="1171"
                     /score="30.1"
                     /tool="antismash"
                     /translation="MHHIISDGVSVQLLIREFTDLY"
     CDS_motif       complement(2527..2640)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0012"
                     /evalue="6.90E-15"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1114"
                     /protein_start="1076"
                     /score="47.4"
                     /tool="antismash"
                     /translation="AYNMPAVLELEGALDVAKLSAVCKELISRHEPLRTSFV"
     aSDomain        complement(2749..2955)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21930_PCP.1"
                     /evalue="4.50E-24"
                     /label="CE047_RS21930_PCP.1"
                     /locus_tag="CE047_RS21930"
                     /protein_end="1040"
                     /protein_start="971"
                     /score="76.5"
                     /tool="antismash"
                     /translation="EAKLSQLFEDVLKNGHVGIQDNFFDNGGHSLKATVLMSRIAKEFH
                     VQVSLKDIFAHPTVEGLALIIREA"
     aSModule        complement(2749..5847)
                     /complete
                     /domains="nrpspksdomains_CE047_RS21930_Condensation_DCL.1"
                     /domains="nrpspksdomains_CE047_RS21930_AMP-binding.1"
                     /domains="nrpspksdomains_CE047_RS21930_PCP.1"
                     /locus_tags="CE047_RS21930"
                     /monomer_pairings="Pro -> Pro"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(3244..3309)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0011"
                     /evalue="6.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CE047_RS21930"
                     /protein_end="875"
                     /protein_start="853"
                     /score="37.4"
                     /tool="antismash"
                     /translation="YIGRADNQVKIRGYRIEPQEIE"
     aSDomain        complement(3274..4464)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21930_AMP-binding.1"
                     /evalue="3.50E-104"
                     /label="CE047_RS21930_AMP-binding.1"
                     /locus_tag="CE047_RS21930"
                     /protein_end="865"
                     /protein_start="468"
                     /score="340.6"
                     /specificity="substrate consensus: Pro"
                     /tool="antismash"
                     /translation="FEKQAEKTPDAHAVIDQACSLTYRELNKAANRLARHLRMKGVVRQ
                     EPVAIMMDRSAAFISGVLGILKAGGAIVPVDPHYPADRIRYILHDCGCSHVVSQAHLSS
                     SLEDNYIITHPEEIESKVDGSNIKSVNDADDLLYMIYTSGSTGKPKGVQFEHRNMANLL
                     KFEYTHSGIDFEADVLQFATPSFDVCYQEIFSALLKGGTLHIVPEDIKRDVPQLFTFIN
                     KHQTNIVFLPTAFIKMIFSERELANSFPDGVKHLIAAGEQLMISDLFQNVLRERGIHLH
                     NHYGPSETHVVSTYTIHPGDPIPELPPIGKPIGCTDLYILNHQKQLQPCGVPGELYISG
                     ASVARGYVNHDKLTSDKFSSDPFRPGVIMYRTGDLARRLEDGNIEYIGRADNQVKIR"
     CDS_motif       complement(3391..3480)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0010"
                     /evalue="6.60E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="CE047_RS21930"
                     /protein_end="826"
                     /protein_start="796"
                     /score="40.8"
                     /tool="antismash"
                     /translation="PCGVPGELYISGASVARGYVNHDKLTSDKF"
     CDS_motif       complement(3589..3615)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0009"
                     /evalue="4.30E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="CE047_RS21930"
                     /protein_end="760"
                     /protein_start="751"
                     /score="4.1"
                     /tool="antismash"
                     /translation="YGPSETHVV"
     CDS_motif       complement(3997..4053)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0008"
                     /evalue="6.50E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="CE047_RS21930"
                     /protein_end="624"
                     /protein_start="605"
                     /score="27.9"
                     /tool="antismash"
                     /translation="YMIYTSGSTGKPKGVQFEH"
     CDS_motif       complement(4237..4278)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0007"
                     /evalue="5.40E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="CE047_RS21930"
                     /protein_end="544"
                     /protein_start="530"
                     /score="13.4"
                     /tool="antismash"
                     /translation="LGILKAGGAIVPVD"
     CDS_motif       complement(4801..4917)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0006"
                     /evalue="1.90E-20"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="CE047_RS21930"
                     /protein_end="356"
                     /protein_start="317"
                     /score="65.4"
                     /tool="antismash"
                     /translation="QKDRLAAEAYSYHPLYEIQSRSAVKQGLIDHILVFENYP"
     aSDomain        complement(4957..5847)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21930_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="1.10E-117"
                     /label="CE047_RS21930_Condensation_DCL.1"
                     /locus_tag="CE047_RS21930"
                     /protein_end="304"
                     /protein_start="7"
                     /score="384.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QDIYPLSYMQEGMLFHSLLQKDSQAYVEQASFTIEGKVNPQFFQN
                     SINALVERHDIFRTIFISQNVSSPQQVVLRERNVIVLEENITHLNEAEQSQFIEQWKEK
                     DRDRGFHLQKDVLMRIALIQTGESQYSCIWTFHHIMMDGWCLSIVLKEFLHIYASYVNA
                     SPITLEPVQPYGKYIKWLMEQDKEQAVSYWDHYLSGHEQQTVLPKQKKTKGKSRQEHVT
                     FSFSKEESSRLSELAAREEVTLSTIFHTIWGILLQKYNNNDDAVFGSVISGRPAEIEGI
                     EHMVGLFINTMPVRVQ"
     CDS_motif       complement(4963..5058)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0005"
                     /evalue="1.00E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="CE047_RS21930"
                     /protein_end="302"
                     /protein_start="270"
                     /score="62.9"
                     /tool="antismash"
                     /translation="DAVFGSVISGRPAEIEGIEHMVGLFINTMPVR"
     CDS_motif       complement(5293..5331)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0004"
                     /evalue="1.20E-02"
                     /label="C4_DCL_171-183"
                     /locus_tag="CE047_RS21930"
                     /protein_end="192"
                     /protein_start="179"
                     /score="8.6"
                     /tool="antismash"
                     /translation="PYGKYIKWLMEQD"
     CDS_motif       complement(5377..5442)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0003"
                     /evalue="5.10E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="CE047_RS21930"
                     /protein_end="164"
                     /protein_start="142"
                     /score="34.9"
                     /tool="antismash"
                     /translation="FHHIMMDGWCLSIVLKEFLHIY"
     CDS_motif       complement(5662..5775)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0002"
                     /evalue="1.30E-15"
                     /label="C2_DCL_024-062"
                     /locus_tag="CE047_RS21930"
                     /protein_end="69"
                     /protein_start="31"
                     /score="49.6"
                     /tool="antismash"
                     /translation="AYVEQASFTIEGKVNPQFFQNSINALVERHDIFRTIFI"
     aSDomain        complement(5779..5868)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21930_NRPS-COM_Nterm.1"
                     /evalue="1.90E-18"
                     /label="CE047_RS21930_NRPS-COM_Nterm.1"
                     /locus_tag="CE047_RS21930"
                     /protein_end="30"
                     /protein_start="0"
                     /score="57.4"
                     /tool="antismash"
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLQKDS"
     CDS_motif       complement(5797..5838)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21930_0001"
                     /evalue="9.10E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="CE047_RS21930"
                     /protein_end="24"
                     /protein_start="10"
                     /score="14.5"
                     /tool="antismash"
                     /translation="YPLSYMQEGMLFHS"
     gene            complement(5893..>9426)
                     /gene="ppsC"
                     /locus_tag="CE047_RS21935"
                     /pseudo=""
     CDS             complement(5893..>9426)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (132-525). E-value:
                     3.7e-115. Score: 376.7. Matches aSDomain:
                     nrpspksdomains_CE047_RS21935_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (630-698). E-value: 2.5e-24. Score:
                     77.3. Matches aSDomain: nrpspksdomains_CE047_RS21935_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (708-1010). E-value:
                     2.2e-168. Score: 553.4. Matches aSDomain:
                     nrpspksdomains_CE047_RS21935_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1016-1170). E-value: 7.7e-53.
                     Score: 170.4. Matches aSDomain:
                     nrpspksdomains_CE047_RS21935_TIGR01720.1"
                     /codon_start=1
                     /gene="ppsC"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389714.1"
                     /locus_tag="CE047_RS21935"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="plipastatin non-ribosomal peptide synthetase
                     PpsC"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 5.6e-123, bitscore:
                     397.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.7e-16, bitscore:
                     47.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 9.5e-80, bitscore:
                     254.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ELTRDMSRNPLFDVMFVLQNMDQESLELDELCLKPAANNGHQTSK
                     FDLTLYAQEQPHGLLTFQMEFSTDLYKKKTIEKWLQYFINMLLSIIKDNKAALGTINIL
                     NEDETHYLIHELNRTKIDYPRNETISRLFEMQAEQTPNAVAIVSDTQVFTYEDLNSWAN
                     QIASVLQIKGVGPDSVVALLTGRTPELIAGMLGILKAGGAYLPIASDLPVERIAYMLSD
                     SGTALLLQSEKTEKQLLDIECEQIIIEDIQKQGKTKNVDSSAGPHSLAYIIYTSGSTGK
                     PKGVMIEQRSVIRLVKNSNYITFTPEDRLLMTSSIGFDVGSFEIFGPLLNGAALHLSDQ
                     QTFLDSHQLKRYIEHQGITTIWLTSSLFNHLTEQNEQTFSQLKHLIIGGEALSPSHVNR
                     IRNVCPEVSIWNGYGPTENTTFSTCLHIQKTYELSIPIGRPVGNSTAYILNQWGVLQPV
                     GAVGELCVGGDGVARGYLGRPDLTKEKFVPNPFAPGDRLYRTGDLARWLPDGTIEYVGR
                     IDDQVKVRGYRVELGEIESALRQIDGVKEAAVLARTAQTGSKELFGYISVKAGTNAEQV
                     RSLLARSLPNYMIPAYIIEMETLPLTSNGKLNRKALPEPNFTSKQTYAPPRNDLEDQLV
                     LIWQEVLGIQRIGIEDSFFELGGDSIKALQVSARLGRYGLSLQVSDLFRHPKIKDLSPF
                     IRKSERIIEQGPVQGDVPWTPVQQWFFSQDIEERHHFNQSVMLFHSGRLSENAIRPALK
                     KLAEHHDALRMVYRNDDRRWIQINQGIHESQLYSLRTSDLSQSESGWETKIKQEVADLQ
                     QSINLQEGPLLHAALFKTLTGDYLFLAIHHLVVDGVSWRILLEDLSAGYQQAAAGQTIQ
                     LPPKTDSYQEYARRIQEYAQSSKLIREEAYWRSVEEQQAAELPYEIPHHVNIDFSKRDS
                     LSFSLTEADTAVLLQNVNHAYGTDTQDILLTAASLAICEWTGGSKLRIAMEGHGREHIL
                     PELDISRTVGWFTSMYPALISFENHRDELGTSVKTVKDTLGRIPNKGVGYGMLKYLTHP
                     ENKSITFSKTPEISFNYLGQFNDIERQDTFRPSSLGSGKDITHTWKREQIIEMSAMAAD
                     KKLHFNLSYPPARFHRNTMEQLINRIEHFLLDIMKHCAGQQKAEKTLSDFSSQSLTAED
                     LDSISSLVEEL"
     aSDomain        complement(5914..6375)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21935_TIGR01720.1"
                     /evalue="7.70E-53"
                     /label="CE047_RS21935_TIGR01720.1"
                     /locus_tag="CE047_RS21935"
                     /protein_end="1170"
                     /protein_start="1016"
                     /score="170.4"
                     /tool="antismash"
                     /translation="ELGTSVKTVKDTLGRIPNKGVGYGMLKYLTHPENKSITFSKTPEI
                     SFNYLGQFNDIERQDTFRPSSLGSGKDITHTWKREQIIEMSAMAADKKLHFNLSYPPAR
                     FHRNTMEQLINRIEHFLLDIMKHCAGQQKAEKTLSDFSSQSLTAEDLDSI"
     aSModule        complement(5914..9027)
                     /complete
                     /domains="nrpspksdomains_CE047_RS21935_AMP-binding.1"
                     /domains="nrpspksdomains_CE047_RS21935_PCP.1"
                     /domains="nrpspksdomains_CE047_RS21935_Epimerization.1"
                     /domains="nrpspksdomains_CE047_RS21935_TIGR01720.1"
                     /locus_tags="CE047_RS21935"
                     /monomer_pairings="Val -> D-Val"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(6220..6249)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21935_0009"
                     /evalue="3.70E-03"
                     /label="NRPS-E7"
                     /locus_tag="CE047_RS21935"
                     /protein_end="1068"
                     /protein_start="1058"
                     /score="9.8"
                     /tool="antismash"
                     /translation="PEISFNYLGQ"
     CDS_motif       complement(6289..6351)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21935_0008"
                     /evalue="1.10E-09"
                     /label="NRPS-E6"
                     /locus_tag="CE047_RS21935"
                     /protein_end="1045"
                     /protein_start="1024"
                     /score="30.4"
                     /tool="antismash"
                     /translation="VKDTLGRIPNKGVGYGMLKYL"
     aSDomain        complement(6394..7299)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21935_Epimerization.1"
                     /evalue="2.20E-168"
                     /label="CE047_RS21935_Epimerization.1"
                     /locus_tag="CE047_RS21935"
                     /protein_end="1010"
                     /protein_start="708"
                     /score="553.4"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="GDVPWTPVQQWFFSQDIEERHHFNQSVMLFHSGRLSENAIRPALK
                     KLAEHHDALRMVYRNDDRRWIQINQGIHESQLYSLRTSDLSQSESGWETKIKQEVADLQ
                     QSINLQEGPLLHAALFKTLTGDYLFLAIHHLVVDGVSWRILLEDLSAGYQQAAAGQTIQ
                     LPPKTDSYQEYARRIQEYAQSSKLIREEAYWRSVEEQQAAELPYEIPHHVNIDFSKRDS
                     LSFSLTEADTAVLLQNVNHAYGTDTQDILLTAASLAICEWTGGSKLRIAMEGHGREHIL
                     PELDISRTVGWFTSMYPALIS"
     CDS_motif       complement(6400..6447)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21935_0007"
                     /evalue="5.50E-09"
                     /label="NRPS-E5"
                     /locus_tag="CE047_RS21935"
                     /protein_end="1008"
                     /protein_start="992"
                     /score="27.9"
                     /tool="antismash"
                     /translation="DISRTVGWFTSMYPAL"
     CDS_motif       complement(6853..6918)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21935_0006"
                     /evalue="1.20E-12"
                     /label="NRPS-E2"
                     /locus_tag="CE047_RS21935"
                     /protein_end="857"
                     /protein_start="835"
                     /score="39.8"
                     /tool="antismash"
                     /translation="LFLAIHHLVVDGVSWRILLEDL"
     CDS_motif       complement(7261..7287)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21935_0005"
                     /evalue="3.20E-03"
                     /label="NRPS-E1"
                     /locus_tag="CE047_RS21935"
                     /protein_end="721"
                     /protein_start="712"
                     /score="10.7"
                     /tool="antismash"
                     /translation="WTPVQQWFF"
     aSDomain        complement(7330..7533)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21935_PCP.1"
                     /evalue="2.50E-24"
                     /label="CE047_RS21935_PCP.1"
                     /locus_tag="CE047_RS21935"
                     /protein_end="698"
                     /protein_start="630"
                     /score="77.3"
                     /tool="antismash"
                     /translation="EDQLVLIWQEVLGIQRIGIEDSFFELGGDSIKALQVSARLGRYGL
                     SLQVSDLFRHPKIKDLSPFIRKS"
     CDS_motif       complement(7819..7884)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21935_0004"
                     /evalue="1.70E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CE047_RS21935"
                     /protein_end="535"
                     /protein_start="513"
                     /score="39.1"
                     /tool="antismash"
                     /translation="YVGRIDDQVKVRGYRVELGEIE"
     aSDomain        complement(7849..9027)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE047_RS21935_AMP-binding.1"
                     /evalue="3.70E-115"
                     /label="CE047_RS21935_AMP-binding.1"
                     /locus_tag="CE047_RS21935"
                     /protein_end="525"
                     /protein_start="132"
                     /score="376.7"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEMQAEQTPNAVAIVSDTQVFTYEDLNSWANQIASVLQIKGVGPD
                     SVVALLTGRTPELIAGMLGILKAGGAYLPIASDLPVERIAYMLSDSGTALLLQSEKTEK
                     QLLDIECEQIIIEDIQKQGKTKNVDSSAGPHSLAYIIYTSGSTGKPKGVMIEQRSVIRL
                     VKNSNYITFTPEDRLLMTSSIGFDVGSFEIFGPLLNGAALHLSDQQTFLDSHQLKRYIE
                     HQGITTIWLTSSLFNHLTEQNEQTFSQLKHLIIGGEALSPSHVNRIRNVCPEVSIWNGY
                     GPTENTTFSTCLHIQKTYELSIPIGRPVGNSTAYILNQWGVLQPVGAVGELCVGGDGVA
                     RGYLGRPDLTKEKFVPNPFAPGDRLYRTGDLARWLPDGTIEYVGRIDDQVKVR"
     CDS_motif       complement(7966..8055)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21935_0003"
                     /evalue="4.10E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="CE047_RS21935"
                     /protein_end="486"
                     /protein_start="456"
                     /score="57.4"
                     /tool="antismash"
                     /translation="PVGAVGELCVGGDGVARGYLGRPDLTKEKF"
     CDS_motif       complement(8560..8616)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21935_0002"
                     /evalue="1.00E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="CE047_RS21935"
                     /protein_end="288"
                     /protein_start="269"
                     /score="33.4"
                     /tool="antismash"
                     /translation="AYIIYTSGSTGKPKGVMIE"
     CDS_motif       complement(8803..8841)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE047_RS21935_0001"
                     /evalue="1.60E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="CE047_RS21935"
                     /protein_end="207"
                     /protein_start="194"
                     /score="15.0"
                     /tool="antismash"
                     /translation="LGILKAGGAYLPI"
ORIGIN
        1 cggcaacagt ctctcaagct gtgcccgcac ttcgtttttt tggagccctt ctacataagc
       61 gcataattcc ggctcgccgc tgtctgtccg tacggtaacg gccgcctctc ttacaccttc
      121 tatacttctt agtgccgctt caatttctcc cggttcaacc cggtagccgc gaattttcac
      181 ctgatcatcc aaccgtccca ggaattcaac attcccgtca gggagccagc gcgccacatc
      241 tcctgttttg tacatgcgtt cacccgggta aaacgggtct tctagaaaat gctcctccgt
      301 taatgccggc cgattcaaat aacctctggc aacacctgct ccggcaatgt acagttcgcc
      361 gatacatcca gatggcagca tccgctgatt ctgattcagg atatacaatc tgacatgatg
      421 aacaggtttt ccaattggaa tccgttcaag tttttcttgc ggcggacaat caaagaatgc
      481 agcttctacg gttgcttccg ttggcccata ggaattggtt attgaaacat ttggaagtaa
      541 ctcgtaaaat cgggagacaa gatgggtccc cagctgttct cctccagaaa acactcgctt
      601 gagtctgttt gtcttaatcg gtacagagcg atattttata tgttctaaaa atacatgaag
      661 cattgaaggc acaaaatgca tagctgtgat cttttgttcc tctatagcct gcgcgatcac
      721 ttcaggttct ttttcgcctc cctgaggaag cagataaaca gaagcaccgg cataagacca
      781 ccaaaacagt tcccatattg aagcgtcaaa tgaataagat gttttatgca taatcacatc
      841 tgattggttc agttgataac gggactgaag ggaattcaaa aaattcaccg ctgatcgatg
      901 ttcaacttca acacccttag gcctcccggt agagccggat gtatagatga cataggctaa
      961 agattgcggg agaacgtcca cattcggatt ggacaaacga tgtttcagcc cgctatttgt
     1021 ctgattgaga tagacaatct cccctgcaaa tgccggaaca tgcaggccgt cagactgaag
     1081 caggagaatg tttgtaccgc tgtcctccag cagaaagctg attcgttctt ccggataatc
     1141 cgggtcaatg ggcagataag cgccgccagc tttaagaatg ccaaaaatgc caatcagcat
     1201 ttcaggcgaa cgccttccca taatggcaac tgttgtttca gattttactc ctcgtccgat
     1261 cagctcccag gcgatacggt tagcctgttc attcaattct ttgtaggtca tcgctccatc
     1321 ttcaaaaaca acagccgtct gatcaggcgt ttttgctgcc tgctgttcga gtgcatagtg
     1381 gagcgtttga ttcattgaat agcccgtctc ctcaaacaga tggcatgatt caatgaaatc
     1441 tcgctcttct tcttttgtta agattggaat attgtccaat tctgtttcag gactggcagc
     1501 tgtatgttcc acaagcgtgg tgaaaaggct ggcccacctt tggatggttt ctttctcaaa
     1561 caaagctgta ctgtattcaa actgacagaa aagctcggaa tcatgttctg taacgtatag
     1621 ggaaatatca aattttgata ttgggtgcac caagtcgttc cactgaagct gtacatcatt
     1681 catttttagc ttttgctgtt ccatattctg aaggactagc gttgtgtcaa acaaaggatt
     1741 ccggctcatt tcccgctgca caccgagttt gtccacaagc tcttcaaacg ggtaatcctg
     1801 atgctcatac gcttctagtg ccgtctgtct gacttctttc agaaattcct caaacgtctt
     1861 attacccgcc gggcgtgtgc ggagagccag tgtattgacg aacatcccaa gaacagcttc
     1921 aaggtcggca tggggccgtc cggcgatagg tgaaccaatc acgatatcat cctgaccgct
     1981 taaccgagac aaaaaggctg aatacaaagc gagaagcgtc atataaagcg tgtttctgtt
     2041 tttatgggcg agccgcttta tgcgggccgt gatctctttg tccaattgaa aggttacttt
     2101 tccgcctatg aattgacgtt ctgccggacg tcgtttatct gttggaagct ccagtatagg
     2161 aagatccccg ctaaactgct gctgccagta agtctcctgc ttttggtacg agtctccctt
     2221 tttaaatttc tgctgccata cggcgtaatc tttatattgg atccgcaatg gcttaagctg
     2281 ccggtttgca taaagatcgg taaattctcg gattaggagc tgaacggata cgccatctga
     2341 aatgatatgg tgcatatcca ccagcaatac atgcttctcg tttgacactt ctataaggcc
     2401 tgctctaaat aatggcgcct ggctgagatc aaagggacgg acaaatccct cgatagtggt
     2461 ctctttactt aacgtaaacg gcacttcggt gtggatacgc tgaaccggct cgtcatcagc
     2521 tccggataca aaggaagttc gaagcggctc atgccggctg atcagttctt tacatacagc
     2581 agacagtttg gcgacatcca gtgcaccttc cagttctaag accgcaggca tgttataggc
     2641 gacaccctca tcaagctgtt gaagcacata gattcgtttt tgggccgaag atacaggata
     2701 tgtatccctt ttttctgctg gttcaattgc tgcatacgga ttttgttctg cctctcgaat
     2761 gattaaagct aatccttcaa cagtcggatg agcaaagatg tccttaagtg aaacttgcac
     2821 atggaattcc tttgcgatcc gtgacataag cactgttgct tttaacgagt ggccgccatt
     2881 gtcaaaaaag ttgtcttgga tgcctacatg cccattcttt agcacatctt caaatagttg
     2941 cgaaagcttt gcctctaaca gatttcgagg ggcagtatat gggtttccgc tgattgaagc
     3001 atctggctcc ggaagtgctg cgcgatctac ttttccgttt ggggtaagag gtatgctgtc
     3061 aacccatacc catttcgccg gaatcatgta ttctgggagc tcactagcca tataactgcg
     3121 taaatctatt gcattcagct tttgtacact gcaatagtaa gcgcataatt catgttcacc
     3181 gttttgatct tgccagatga gaatggcagc ctcactaatg tccggatgat tcattagcgt
     3241 gacttcgatt tcttgcggct caattcgata acctctgatt ttcacttgat tgtctgcccg
     3301 tccgatatat tcgatgttgc cgtcttcaag ccgtctggcc aaatcacctg tccggtacat
     3361 aatcacacca ggcctgaatg ggtcggatga aaatttgtcg cttgtcagtt tatcgtgatt
     3421 gacatatcct ctggcaacac ttgcgccgga aatatagagt tcgcctggta cgccgcatgg
     3481 ctgaagctgt ttttgatgat tcaaaatata tagatcagta cagcctatcg gtttaccgat
     3541 tggcggaagc tcaggaatag ggtctccagg atgaatggta tacgtagata ccacatgagt
     3601 ttctgaaggt ccgtaatgat tgtgcaggtg tatgccgcgt tcacgcaaca cattttggaa
     3661 aaggtctgaa atcattaatt gttccccggc tgcgatcagg tgtttgacgc catcaggaaa
     3721 cgagttcgca agttctcgct cgctgaaaat cattttgata aaagcagttg ggagaaacac
     3781 aatattcgtc tgatgcttgt ttataaatgt aaacagctga ggcacatctc tttttatgtc
     3841 ttctggaacg atgtggagtg tgccgccttt cagaagcgca gaaaatattt cttgatagca
     3901 gacgtcgaag gaaggtgtcg caaattgcag aacatctgct tcaaagtcaa tgccggagtg
     3961 agtatattcg aatttcagca agttagccat gtttcgatgc tcaaattgaa caccctttgg
     4021 tttacctgtt gagcctgatg tataaatcat atacagcaga tcgtcagcat cgttgactga
     4081 ctttatgtta ctgccgtcta ctttgctttc gatttcttct ggatgagtga taatgtaatt
     4141 gtcttctaac gatgaggaaa gatgcgcttg tgaaacaaca tgcgaacagc cgcaatcatg
     4201 cagaatataa cggattctgt cagccgggta atgcggatca acgggaacaa tggcgccgcc
     4261 tgcctttaag attccaagaa cgccgctgat aaacgccgca gagcgatcca tcataatcgc
     4321 aacgggttcc tgtctcacaa cgcctttcat tcgtaaatgc cgtgccaatc tgttagccgc
     4381 tttattcagt tctctgtatg ttaatgagca ggcttgatca attacagcat gagcatctgg
     4441 tgtcttttcc gcttgctttt caaacatggc atggatggtt tcgtaaactg gtgttcgatc
     4501 tgtgatttct ccaatcagct gccgcttttc agtatctgtc gtgatatcga tttcttcagg
     4561 tggaagattc ggattattta agatttgtga tgcagcattc agaagatgct ctttgacaga
     4621 gagcacaaaa gaccgatcgt gctgctccgc atcatagttc attttgatat gaatttcttc
     4681 ccctggcgcc accataagat aaaagctgta gttggtttca tctgcgacag tgaaattatg
     4741 aatctgaaaa agatcagtag catgttcctg tttgttcagc atttgaatct cttgctgcac
     4801 cggatagttt tcaaatacga gaatatgatc aatcagccct tgcttaacag ctgagcggga
     4861 ttgaatctca tataaagggt ggtagctgta cgcttcagcg gcaagacggt ctttttgcat
     4921 atcttttata agttgaagaa atggcgtttt tgccccctgc acccgaaccg gcatcgtatt
     4981 aatgaaaaga cctaccatat gttcaatgcc ttcgatttct gcgggccttc ctgaaatgac
     5041 agaaccgaat acggcgtcat cgttattatt atatttttgc agcagaatgc cccaaatggt
     5101 atgaaaaatc gtactgagtg tcacttcttc tctagcagca agctctgaaa gcctgctgct
     5161 ttcttcctta gagaatgaaa atgtaacgtg ttcctgcctg ctttttccct ttgttttttt
     5221 ctgcttcggg agcacggttt gctgttcgtg gccggaaaga taatggtccc aataagaaac
     5281 cgcctgctct ttgtcttgct ccatcagcca tttgatatac tttccgtatg gctggaccgg
     5341 ctccaatgtt ataggggatg catttacata agatgcgtaa atatgcagaa actcctttag
     5401 cacaatgctg aggcaccaac cgtccatcat gatatgatga aaggtccaaa tgcaggagta
     5461 ctggctctct cctgtttgaa taagagcgat gcgcatgagc acatcctttt gcaaatgaaa
     5521 cccccggtcc cggtcctttt ctttccattg ctcgataaac tgtgattgct ccgcctcgtt
     5581 taaatgagta atgttttctt ccaggacgat gacatttcgt tctctcagaa caacctgctg
     5641 gggggaggaa acattttggc tgataaaaat cgttctgaaa atatcatgtc tttctacaag
     5701 agcgttgatg ctgttttgaa aaaactgcgg gttgactttt ccttctattg taaaagaagc
     5761 ttgctcaaca tatgcctgtg aatctttttg caggagggaa tggaaaagca tcccttcctg
     5821 catgtaagac agcggataaa tatcctgaat tgaattcgct tttgtcatgt tcatgtctcc
     5881 tgtcacgatt ttctacaatt cttccaccaa gctggatatg ctgtccaagt cctctgccgt
     5941 taatgattga ctgctgaaat cgctcagtgt cttttctgct ttctgttgac cggcgcagtg
     6001 tttcataatg tccagcaaaa agtgttcaat cctgttaata agctgctcca ttgtgtttct
     6061 atgaaagcgg gctggcggat aactaagatt gaaatggagc tttttgtctg ctgccattgc
     6121 actcatctca atgatttgct cacgcttcca tgtatgagtg atatcctttc cgcttccaag
     6181 gcttgatgga cggaaggtgt cctgtctttc gatgtcgttg aactggccca aatagttaaa
     6241 gctgatttca ggtgtttttg aaaacgtaat gcttttattt tcaggatgtg taaggtattt
     6301 cagcataccg taacctaccc ctttgtttgg tatccggccc agtgtatctt taacggtttt
     6361 tactgaggtt ccgagctcat ccctatgatt ttcaaagctt atgagggccg ggtacatgga
     6421 tgtaaaccag ccgaccgtcc tgctgatatc taactctggc agaatgtgtt ctctcccgtg
     6481 tccttccata gcgatacgga gcttgcttcc tcctgtccat tcacaaatcg ccaaggatgc
     6541 tgcggttaac agaatatctt gtgtatctgt gccataggcg tgattgacgt tttgcagcaa
     6601 gacggcggtg tctgcttcag tcagtgaaaa gcttaatgag tcccttttgc tgaagtcgat
     6661 gttcacatga tgcggtattt catatggcag ttctgcggct tgctgttctt ctacacttcg
     6721 ccaatacgcc tcttcgcgaa tcagcttact gctttgggca tattcctgta tgcgtcttgc
     6781 gtattcctgg taggaatccg ttttaggtgg aagctggatc gtctggcctg cagcagcttg
     6841 ttgatatccg gctgacaaat cctccagcaa aatccgccat gagacaccat caacgacaag
     6901 gtgatggatc gcaaggaaca agtaatctcc ggtcagtgtt ttaaacaaag cggcgtgcaa
     6961 caatggtcct tcctgcaaat tgatgctttg ctgaagatct gcaacttctt gtttgatttt
     7021 cgtctcccag ccgctttctg actgtgaaag atcagaggtt cttaagctgt atagctgtga
     7081 ctcatgaatg ccttggttga tttgaatcca tcgcctatca tcgttgcgat aaaccatccg
     7141 aagcgcatca tgatgctccg ccagtttttt caaggctggt ctaatggcgt tttcagataa
     7201 acgtccagaa tggaatagca tgacagactg attgaaatgg tgtctttctt caatatcttg
     7261 tgaaaaaaac cattgctgaa caggcgtcca aggaacgtct ccctgaacgg gtccttgctc
     7321 tatgattctc tctgacttac ggataaatgg tgataagtcc tttatctttg gatgacggaa
     7381 taaatcactt acctgcaagc ttagtccata gcgcccaagg cgtgcagaaa cttgaagggc
     7441 tttgattgaa tctccgccca attcaaaaaa cgaatcctct attccgatcc tctggatgcc
     7501 gagaacttcc tgccagatca acacaagttg gtcttcaagg tcatttcgag gtggagcgta
     7561 cgtttgttta gatgtgaaat ttggttcagg aagtgctttt cggttcaatt tgccgtttga
     7621 tgtgagcggg agagtctcca tttcaataat gtacgccggg atcatatagt ttggcagcga
     7681 acgcgcgaga agtgaacgta cttgttcagc attcgtgcct gcttttacgc tgatatagcc
     7741 aaacagctct ttgctcccgg tctgggctgt acgtgctaac actgcggctt ctttgacccc
     7801 gtctatttga cgaagtgctg attcgatttc acccagctcc acccgatagc ctctgacttt
     7861 tacttgatca tcaatacgcc caacgtactc aattgtgcca tcaggaagcc agcgcgccag
     7921 atcacctgtg cgatacagtc gatcgccagg tgcaaatgga tttgggacaa atttctcttt
     7981 cgttagatca ggacggccca aatatcctct ggcgacgcca tctccgccga cacaaagttc
     8041 accgacagct ccgaccggct gcagcactcc ccattgattt aagatataag cggtggaatt
     8101 accaaccggc cgcccgatcg gaatggaaag ttcatatgtt ttttgaatgt gaaggcatgt
     8161 cgaaaaagtt gtattttcgg tagggccata tccattccat atagatactt cagggcagac
     8221 atttctgatt cgattgacgt ggctcggcga caaagcttct ccgcctataa tcaaatgttt
     8281 cagctgtgaa aatgtttgtt cattttgttc tgtcaaatga ttgaatagag aagatgtcag
     8341 ccaaatcgtt gtgattcctt gatgttctat gtacctctta agctgatggg aatcgagaaa
     8401 tgtttgttga tcggacagat gcaaagcggc accatttagc agcggtccga aaatttcaaa
     8461 tgacccaacg tcgaaaccga ttgatgatgt cataagaagg cggtcttcag gtgtaaatgt
     8521 aatataattg ctgtttttaa caagacgaat tacacttcgc tgctcaatca tgacaccctt
     8581 tggtttaccg gttgatccag aggtataaat gatatatgca agtgaatgtg gccctgctga
     8641 agaatcaacg ttcttcgttt ttccttgctt ttgaatatct tcgattatga tttgttcaca
     8701 ttcaatgtct aatagctgtt tttctgtttt ttcagattga aggagtaatg ccgtcccgct
     8761 gtctgacagc atataagcta ttcgttcaac aggcagatca gaagcaatag gcagataggc
     8821 tcccccggct ttcagaatgc ccagcattcc tgcaatgagt tctggtgttc tgcctgtcaa
     8881 caaggctaca acactatcag gccctacacc cttaatctgt agaactgagg caatttgatt
     8941 tgcccagctg ttcaggtcct cgtaagtgaa tacttgggtg tcgctgacaa ttgctacagc
     9001 attaggcgtt tgttcagcct gcatttcaaa gagtctgctg atggtttcat ttcttggata
     9061 atctattttt gtacgattga gctcgtggat aaggtaatgg gtctcatcct cattcaggat
     9121 atttattgtt ccgagagcgg ctttgttgtc tttgatgatg gagagtaaca tattgatgaa
     9181 gtattgaagc cacttttcta tcgttttttt cttatataag tcagtactaa actccatttg
     9241 gaacgtcagc aaaccgtggg gctgttcctg ggcatatagg gttaaatcaa atttggacgt
     9301 ttgatgccca ttgtttgctg caggtttgag acaaagttca tccagttcca acgattcctg
     9361 atccatgttt tgaagaacaa acattacgtc aaacagcgga tttctgctca tatcccgtgt
     9421 caattc
//
