LOCUS       NZ_NJRD01000016        14013 bp    DNA     linear   CON 22-JUN-2024
DEFINITION  Bacillus subtilis strain 20-3 20-3_R1_(paired)_contig_16, whole
            genome shotgun sequence.
ACCESSION   NZ_NJRD01000016
VERSION     NZ_NJRD01000016.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Cui,Y.
  TITLE     Improper usage of probiotics constitutes a potential risk of public
            health
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14013)
  AUTHORS   Cui,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2017) Beijing Advanced Innovation Center for Food
            Nutrition and Human Health, College of Veterinary Medicine, China
            Agricultural University, Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 9.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010578135.1-RS_2024_06_22
            Annotation Date                   :: 06/22/2024 02:42:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,274
            CDSs (total)                      :: 4,198
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 80 of 134
            Pseudo Genes (incomplete)         :: 67 of 134
            Pseudo Genes (internal stop)      :: 22 of 134
            Pseudo Genes (multiple problems)  :: 31 of 134
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:13:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 14013
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NJRD01000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..14013
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     gene            <1..3718
                     /gene="ppsA"
                     /locus_tag="CE053_RS21155"
                     /pseudo=""
     source          1..14013
                     /collection_date="2016-05"
                     /db_xref="taxon:1423"
                     /geo_loc_name="China"
                     /mol_type="genomic DNA"
                     /note="commercial probiotic used for animal"
                     /organism="Bacillus subtilis"
                     /strain="20-3"
                     /submitter_seqid="20-3_R1_(paired)_contig_16"
     cand_cluster    1..14013
                     /SMILES="NC(CCCN)C(=O)NC(Cc1ccc(O)cc1)C(=O)NC(C(O)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..14013
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<2:>14013](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <3..>14013
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             <3..3718
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (180-585). E-value: 3.9e-96.
                     Score: 314.0. Matches aSDomain:
                     nrpspksdomains_CE053_RS21155_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (690-754). E-value: 2.7e-21. Score:
                     67.5. Matches aSDomain: nrpspksdomains_CE053_RS21155_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (766-1077). E-value:
                     1.7e-149. Score: 490.9. Matches aSDomain:
                     nrpspksdomains_CE053_RS21155_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1085-1230). E-value: 1.9e-46.
                     Score: 149.7. Matches aSDomain:
                     nrpspksdomains_CE053_RS21155_TIGR01720.1"
                     /codon_start=2
                     /gene="ppsA"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="CE053_RS21155"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="plipastatin non-ribosomal peptide synthetase
                     PpsA"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 1.5e-106, bitscore:
                     344.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.4e-15, bitscore:
                     45.4, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.1e-88, bitscore:
                     284.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LGMFVNTLALRTRPEGGKPFVQYLQEVRETALEAFEHQDYPFEEL
                     VDKLELTRDMSRNPVFDAMFILQNVEKQDIDLREIKVRPANFAHHISLFDITLIATEIN
                     GSICCEMEFSTEVFLKATIERWADHFTEFLHAALSTPETSLAQINILSDKEKQKIVFEF
                     NKTQVEFAQKDIPFHRIFEAKAEENSEHIAVIDNETEISYRLLNERANRLARTLQNRKG
                     PKPTVAVLAKRSIDAIVGVLAVMKAGGVYIPIDSHYPKARIEYILRDSGADILLLQREL
                     KHLISNSPESEMSHIFLDDEGSFEESNCNLNLSPAPEEPVYIIYTSGTTGAPKGVIVTY
                     QNFTHAALAWRQIYELDRKPVRLLQIASFSFDVFSGDLARTLTNGGTLIVCPDETRLEP
                     AEIYKIMNSRRITVMESTPALIIPVMEYVYRNQFKLPDLDILILGSDMVKAQDFKTLTD
                     RFGQSMRIINSYGVTEATIDSSFYETSMGGECTGDNVPIGSPLPNVHMYVLSQTDQIQP
                     IGVAGELCIGGAGVAKGYHHKPDLTQMKFTENPFVSGERLYRTGDRACWLPNGTIRLLG
                     RMDYQVKINGYRIETKEIESVLLQTGLVREAAVAVQHDKNGQAGLVAYIVPSDVNTNAL
                     RAALTKELPAYMIPAHLIPLENMPLTLNGKLDRNALPAPNNVLSRPYTAPVNDIQKTMA
                     YIWEDVLSMSRVGIHDSFFELGGDSIKALQVAARLAAEGWSMTIRDLFRYSTIQELCGH
                     ITPLASQADQGPAEGEAELTPIQRRFFGQVHAFHYHYNQSVMLFSEKGFNANALHLALR
                     KITEHHDAIRMIFQRDQNGHVIQFNRGINHKDHELFGLYISDWTKASLERAHLDEKLDA
                     EETVIQSKMNVEKGPLLQAGLFKTAEGDHLLIALHHLVIDGVSWRILLEDLAAAYQQAL
                     EKKEIQLPPKTDSYLSYADGLTQIAESKQLLSEKPYWQTILDAHTAFLPKDIENVPDRL
                     QMNSDAAAFVLSGDWTEKLLFETQQAYGTDANELLLTALGMALSEWTGHDQIVISTEGH
                     GREGHVPNIDISRTVGWFTSIYPILLDMGIPEPFEDQLAYRIKTTKDMLRRVPNKGTGY
                     GLLTHIGELRHKEPEVSFNYLGQFSEEKEADTFELSYYKPRYEIAGEREREYELDINAL
                     ITDGRLQVKAIYTQVFSKHSIECFMDRFHRHLIETIEHCSQKKAREKTLSDFSNKELTL
                     SALSSIEDLVKDL"
     CDS_motif       86..205
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0001"
                     /evalue="5.80E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CE053_RS21155"
                     /protein_end="67"
                     /protein_start="27"
                     /score="66.9"
                     /tool="antismash"
                     /translation="RETALEAFEHQDYPFEELVDKLELTRDMSRNPVFDAMFIL"
     aSDomain        545..1759
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21155_AMP-binding.1"
                     /evalue="3.90E-96"
                     /label="CE053_RS21155_AMP-binding.1"
                     /locus_tag="CE053_RS21155"
                     /protein_end="585"
                     /protein_start="180"
                     /score="314.0"
                     /specificity="substrate consensus: Orn"
                     /tool="antismash"
                     /translation="FEAKAEENSEHIAVIDNETEISYRLLNERANRLARTLQNRKGPKP
                     TVAVLAKRSIDAIVGVLAVMKAGGVYIPIDSHYPKARIEYILRDSGADILLLQRELKHL
                     ISNSPESEMSHIFLDDEGSFEESNCNLNLSPAPEEPVYIIYTSGTTGAPKGVIVTYQNF
                     THAALAWRQIYELDRKPVRLLQIASFSFDVFSGDLARTLTNGGTLIVCPDETRLEPAEI
                     YKIMNSRRITVMESTPALIIPVMEYVYRNQFKLPDLDILILGSDMVKAQDFKTLTDRFG
                     QSMRIINSYGVTEATIDSSFYETSMGGECTGDNVPIGSPLPNVHMYVLSQTDQIQPIGV
                     AGELCIGGAGVAKGYHHKPDLTQMKFTENPFVSGERLYRTGDRACWLPNGTIRLLGRMD
                     YQVKIN"
     aSModule        545..3694
                     /complete
                     /domains="nrpspksdomains_CE053_RS21155_AMP-binding.1"
                     /domains="nrpspksdomains_CE053_RS21155_PCP.1"
                     /domains="nrpspksdomains_CE053_RS21155_Epimerization.1"
                     /domains="nrpspksdomains_CE053_RS21155_TIGR01720.1"
                     /locus_tags="CE053_RS21155"
                     /monomer_pairings="Orn -> D-Orn"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       728..769
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0002"
                     /evalue="7.40E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="CE053_RS21155"
                     /protein_end="255"
                     /protein_start="241"
                     /score="16.0"
                     /tool="antismash"
                     /translation="LAVMKAGGVYIPID"
     CDS_motif       968..1021
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0003"
                     /evalue="1.20E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="CE053_RS21155"
                     /protein_end="339"
                     /protein_start="321"
                     /score="24.0"
                     /tool="antismash"
                     /translation="YIIYTSGTTGAPKGVIVT"
     CDS_motif       1553..1642
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0004"
                     /evalue="1.30E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="CE053_RS21155"
                     /protein_end="546"
                     /protein_start="516"
                     /score="46.3"
                     /tool="antismash"
                     /translation="PIGVAGELCIGGAGVAKGYHHKPDLTQMKF"
     CDS_motif       1727..1789
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0005"
                     /evalue="3.60E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="CE053_RS21155"
                     /protein_end="595"
                     /protein_start="574"
                     /score="28.9"
                     /tool="antismash"
                     /translation="LGRMDYQVKINGYRIETKEIE"
     CDS_motif       1886..2017
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0006"
                     /evalue="1.20E+00"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE053_RS21155"
                     /protein_end="671"
                     /protein_start="627"
                     /score="1.6"
                     /tool="antismash"
                     /translation="SDVNTNALRAALTKELPAYMIPAHLIPLENMPLTLNGKLDRNAL"
     aSDomain        2075..2266
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21155_PCP.1"
                     /evalue="2.70E-21"
                     /label="CE053_RS21155_PCP.1"
                     /locus_tag="CE053_RS21155"
                     /protein_end="754"
                     /protein_start="690"
                     /score="67.5"
                     /tool="antismash"
                     /translation="KTMAYIWEDVLSMSRVGIHDSFFELGGDSIKALQVAARLAAEGWS
                     MTIRDLFRYSTIQELCGHI"
     aSDomain        2303..3235
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21155_Epimerization.1"
                     /evalue="1.70E-149"
                     /label="CE053_RS21155_Epimerization.1"
                     /locus_tag="CE053_RS21155"
                     /protein_end="1077"
                     /protein_start="766"
                     /score="490.9"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVIDG"
                     /tool="antismash"
                     /translation="EGEAELTPIQRRFFGQVHAFHYHYNQSVMLFSEKGFNANALHLAL
                     RKITEHHDAIRMIFQRDQNGHVIQFNRGINHKDHELFGLYISDWTKASLERAHLDEKLD
                     AEETVIQSKMNVEKGPLLQAGLFKTAEGDHLLIALHHLVIDGVSWRILLEDLAAAYQQA
                     LEKKEIQLPPKTDSYLSYADGLTQIAESKQLLSEKPYWQTILDAHTAFLPKDIENVPDR
                     LQMNSDAAAFVLSGDWTEKLLFETQQAYGTDANELLLTALGMALSEWTGHDQIVISTEG
                     HGREGHVPNIDISRTVGWFTSIYPILLDMG"
     CDS_motif       2318..2344
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0007"
                     /evalue="4.40E-03"
                     /label="NRPS-E1"
                     /locus_tag="CE053_RS21155"
                     /protein_end="780"
                     /protein_start="771"
                     /score="10.3"
                     /tool="antismash"
                     /translation="LTPIQRRFF"
     CDS_motif       2408..2479
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0008"
                     /evalue="2.10E-02"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE053_RS21155"
                     /protein_end="825"
                     /protein_start="801"
                     /score="7.3"
                     /tool="antismash"
                     /translation="FNANALHLALRKITEHHDAIRMIF"
     CDS_motif       2705..2770
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0009"
                     /evalue="2.50E-11"
                     /label="NRPS-E2"
                     /locus_tag="CE053_RS21155"
                     /protein_end="922"
                     /protein_start="900"
                     /score="35.7"
                     /tool="antismash"
                     /translation="LLIALHHLVIDGVSWRILLEDL"
     CDS_motif       3176..3226
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0010"
                     /evalue="4.40E-09"
                     /label="NRPS-E5"
                     /locus_tag="CE053_RS21155"
                     /protein_end="1074"
                     /protein_start="1057"
                     /score="28.3"
                     /tool="antismash"
                     /translation="DISRTVGWFTSIYPILL"
     aSDomain        3260..3694
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21155_TIGR01720.1"
                     /evalue="1.90E-46"
                     /label="CE053_RS21155_TIGR01720.1"
                     /locus_tag="CE053_RS21155"
                     /protein_end="1230"
                     /protein_start="1085"
                     /score="149.7"
                     /tool="antismash"
                     /translation="LAYRIKTTKDMLRRVPNKGTGYGLLTHIGELRHKEPEVSFNYLGQ
                     FSEEKEADTFELSYYKPRYEIAGEREREYELDINALITDGRLQVKAIYTQVFSKHSIEC
                     FMDRFHRHLIETIEHCSQKKAREKTLSDFSNKELTLSALSS"
     CDS_motif       3281..3340
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21155_0011"
                     /evalue="9.10E-09"
                     /label="NRPS-E6"
                     /locus_tag="CE053_RS21155"
                     /protein_end="1112"
                     /protein_start="1092"
                     /score="27.6"
                     /tool="antismash"
                     /translation="TKDMLRRVPNKGTGYGLLTH"
     aSDomain        3743..3829
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21160_NRPS-COM_Nterm.1"
                     /evalue="1.60E-15"
                     /label="CE053_RS21160_NRPS-COM_Nterm.1"
                     /locus_tag="CE053_RS21160"
                     /protein_end="29"
                     /protein_start="0"
                     /score="48.0"
                     /tool="antismash"
                     /translation="MAQSAQIQDIYPLSHMQEGMLFHSLMDFS"
     gene            3743..11425
                     /gene="ppsB"
                     /locus_tag="CE053_RS21160"
     CDS             3743..11425
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 1.6e-15.
                     Score: 48.0. Matches aSDomain:
                     nrpspksdomains_CE053_RS21160_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (7-309). E-value:
                     2.2e-111. Score: 363.7. Matches aSDomain:
                     nrpspksdomains_CE053_RS21160_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (474-865). E-value:
                     1.5e-109. Score: 358.3. Matches aSDomain:
                     nrpspksdomains_CE053_RS21160_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1036). E-value: 4.6e-23. Score:
                     73.2. Matches aSDomain: nrpspksdomains_CE053_RS21160_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1054-1341). E-value:
                     2.3e-103. Score: 337.3. Matches aSDomain:
                     nrpspksdomains_CE053_RS21160_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1505-1903). E-value:
                     1.6e-116. Score: 381.2. Matches aSDomain:
                     nrpspksdomains_CE053_RS21160_AMP-binding.2"
                     /NRPS_PKS="Domain: PCP (2011-2078). E-value: 2.2e-20.
                     Score: 64.6. Matches aSDomain:
                     nrpspksdomains_CE053_RS21160_PCP.2"
                     /NRPS_PKS="Domain: Epimerization (2088-2392). E-value:
                     4.7e-158. Score: 519.2. Matches aSDomain:
                     nrpspksdomains_CE053_RS21160_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (2403-2553). E-value: 1.8e-53.
                     Score: 172.5. Matches aSDomain:
                     nrpspksdomains_CE053_RS21160_TIGR01720.1"
                     /codon_start=1
                     /gene="ppsB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389715.1"
                     /locus_tag="CE053_RS21160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="plipastatin non-ribosomal peptide synthetase
                     PpsB"
                     /protein_id="WP_097080611.1"
                     /sec_met_domain="Condensation (E-value: 9e-95, bitscore:
                     304.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.9e-125, bitscore:
                     405.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.5e-17, bitscore:
                     51.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQSAQIQDIYPLSHMQEGMLFHSLMDFSSKAYIEQTSFTITGNL
                     CVDSFQKSLNLLVSRYDIFRTIFIKEVPDLTGPQQVVLSNRELTVYREDISRLADQEQQ
                     ALIDAFMTKDREKGFDLQKDPLMRLALFDRGDSQYTCVWTHHHIIMDGWCLGIILKEFF
                     SMYDSLKNNSPVQLGSTVPYSRYIEWLGEQDQEETAAYWSEYLKEYGNTASIPRIRRRT
                     AEGDYKAAQISFSLAPDMVEKLTEAAQNWGVTLNTLFMSIWGVLLHRYNAADDAVFGSV
                     ISGRPSAIDGIESMVGLFINTVPVRIRSAEGITFSSLIKAVQEDILSSEQHGYYPLYEI
                     QNHSPLKQGLIDHIFVFENYPVQLYQALSVESENDEGALKLSDISMSEQTNYDFNIVIV
                     PGESFYIKFSYNADVYEREEMLRIQGHLKQALDCILTNPDVAVSDINIVPPEEQQVIQS
                     FNETERPYVNKTIPQLFEEQAHKTPEAAALKMGNECWTYRQLQVRANQIAHALIEKGVG
                     SGDIVAVMMGRSMEMPAALLGIWKAGGAYMPLDPHFPAERLSFLLKDSQAAQLLIEEDL
                     ISLIPPSYEGNTITIEHTESYQTEAPNMPPGDLAYLIYTSGTTGRPKGVLVDHHGIANT
                     LQWRREEYGMTEQDISLHLFSYVFDGCVTSLFTPLLSGACVLLTTDDEAKDVLALKRKI
                     ARYKVSHMIIVPSLYRVLLEVMTADDAKSLRIVTFAGEAVTPDLLELSQMICPSAELAN
                     EYGPTENSVATTILRHLNEKERITIGHPIRNTKVFVLHGNQMQPIGVAGELCISGAGLA
                     RGYYKQQELTQKAFSDHPFLEGERLYRTGDAGRFLPDGTIEYIGRFDDQVKIRGYRIEL
                     REIETVLRQAPGVKEAAVLARDVSAEEKELVAYIVPEKGNSLPDLYQHLAGTLPSYMIP
                     ASIINISWMPLTSSGKLDRFALPEPENNTSVTYMAPRTLIEADLAHIWEDVLNKQHIGI
                     RDDFFQLGGQSLKAAALVSRIHKKLNVELPLSEVFSYPTVESMAVKLMSLKEHAFTQIE
                     PADQRDVYPLSFSQKRLYALHQLADDSTGYNMPAVLELRGSLNRQRLRSVLTELVNRHE
                     ALRTVFVLDRDEPVQIIYPEIAFDLKELEMESEQMLESAIETFIKPFDLSSGPLFRACV
                     ITMGNNRGFLLLDMHHIIADGVSMSTLVQEFTDLYCGKELPALNLHYKDFAVWQQEKHP
                     KELYKKQEAYWLGQLGGSLPTLELPIDKTRPRLPDFSGGTIEVNIDKDMADELHRLMAE
                     TGTTLYMILLAVYSILLSKLSGQEDIVVGSPAAGRPHADLDRVIGMFVNTLAMRSKPEG
                     HKTFSSYLQDIRHLALTAYEHQDYPFEELADKLDTNREVNRNPLFDAMLVLQSSEDFRF
                     EVPGLSISSVTPKHDISKFDLTLHAEEHPSGIRCRFEYSTALFEEETITQWASYFIELV
                     KGVTADTEMRISDMQLLPAAERRLLLEKMGQYAAYPRNENIVSLFEKQAAKYPEHIAVV
                     CGHSQLTYRDLNEKAERAAAMLIKQGVRTGDIVGLMLDRSPDMVIGVLSILKAGGAYLP
                     IDPEYPQERISFMLNDSGAKRLLTERGQNKPADYNGHILYIDECENDSIPADVNIQEIV
                     TDQPAYVIYTSGTTGQPKGVIVEHCNVISLLKHQNLPFEFNHEDIWTLFHSYCFDFSVW
                     EMFGALLNGGTLVVVSKETARDPHAFRLLLKKERVTVLNQTPTAFYGLMLEDQNHTDHL
                     NIRYVIFGGEALQPGLLQSWNEKYPHTDLINMYGITETTVHVTFKKLSAADIAKNKSNI
                     GRPLSTLHAHVMDAHMNLQPIGVPGELYIGGEGVARGYLNRDELTADRFVSNPYLPGDR
                     LYRTGDLAKRLSNGELEYLGRIDEQVKVRGHRIELGEIQAALLQYPMIKEAAVITRADE
                     RGQTAIYAYMVIKDQQAANISDIRASLKNALPDFMLPARMIQIDSIPVTVNGKLDQKAL
                     PEPEKHAYTADDISPRNEIETVMAEIWEDLLNVDELGVSANFFELGGDSIKALQVCARL
                     KQRGFETTVREMFEHQTLGELSARVRKDVRAIDQGPVEGEITWTPIQQWFFSQSLESHH
                     FNQSVMIYRTERFDEAALRKVLKSLVTHHDALRIVCRHEDGRQVQINRGIDLSDEELYV
                     LELFDVKDSLTEARNTIEEAASRMQEHIRLETGPLLHAGLFRTENGDHLFLTIHHLVVD
                     AVSWRILFEDFSAAYKQAVSGESIKLPQKTDSYLTYSQRIADYSKSRQVQREAAYWDEY
                     ENRHIQPIPKDNDAASNTFKDTEVIHFELSRHHTELLLTAAHKAYSTEMNDILLTALGL
                     ALQKWTGNNQFKISMEGHGRESYLEDIDISRTVGWFTSIYPVWLDMRDSELKDKEERLG
                     HLIKQTKDMLHRIPHKGAGYGVLKYISKRWGSEKNSPEISFNYLGQFDQDIQSKAFEVS
                     DIKTGNEISPHWERPYILDISGAVSSGCLNMHIIYNRHQFEEETIQTFSRHFKQALDNI
                     IEHCTGKENREWSASDFTDEDLTLDELSEIMGAVNKL"
     aSDomain        3764..4669
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21160_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="2.20E-111"
                     /label="CE053_RS21160_Condensation_DCL.1"
                     /locus_tag="CE053_RS21160"
                     /protein_end="309"
                     /protein_start="7"
                     /score="363.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="QDIYPLSHMQEGMLFHSLMDFSSKAYIEQTSFTITGNLCVDSFQK
                     SLNLLVSRYDIFRTIFIKEVPDLTGPQQVVLSNRELTVYREDISRLADQEQQALIDAFM
                     TKDREKGFDLQKDPLMRLALFDRGDSQYTCVWTHHHIIMDGWCLGIILKEFFSMYDSLK
                     NNSPVQLGSTVPYSRYIEWLGEQDQEETAAYWSEYLKEYGNTASIPRIRRRTAEGDYKA
                     AQISFSLAPDMVEKLTEAAQNWGVTLNTLFMSIWGVLLHRYNAADDAVFGSVISGRPSA
                     IDGIESMVGLFINTVPVRIRS"
     aSModule        3764..6850
                     /complete
                     /domains="nrpspksdomains_CE053_RS21160_Condensation_DCL.1"
                     /domains="nrpspksdomains_CE053_RS21160_AMP-binding.1"
                     /domains="nrpspksdomains_CE053_RS21160_PCP.1"
                     /locus_tags="CE053_RS21160"
                     /monomer_pairings="Tyr -> Tyr"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       3773..3814
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0001"
                     /evalue="9.00E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="CE053_RS21160"
                     /protein_end="24"
                     /protein_start="10"
                     /score="14.5"
                     /tool="antismash"
                     /translation="YPLSHMQEGMLFHS"
     CDS_motif       3836..3949
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0002"
                     /evalue="2.10E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="CE053_RS21160"
                     /protein_end="69"
                     /protein_start="31"
                     /score="45.7"
                     /tool="antismash"
                     /translation="AYIEQTSFTITGNLCVDSFQKSLNLLVSRYDIFRTIFI"
     CDS_motif       4175..4240
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0003"
                     /evalue="2.50E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="CE053_RS21160"
                     /protein_end="166"
                     /protein_start="144"
                     /score="35.9"
                     /tool="antismash"
                     /translation="HHHIIMDGWCLGIILKEFFSMY"
     CDS_motif       4565..4660
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0004"
                     /evalue="2.40E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="CE053_RS21160"
                     /protein_end="306"
                     /protein_start="274"
                     /score="61.7"
                     /tool="antismash"
                     /translation="DAVFGSVISGRPSAIDGIESMVGLFINTVPVR"
     CDS_motif       4709..4825
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0005"
                     /evalue="3.10E-19"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="CE053_RS21160"
                     /protein_end="361"
                     /protein_start="322"
                     /score="61.4"
                     /tool="antismash"
                     /translation="QEDILSSEQHGYYPLYEIQNHSPLKQGLIDHIFVFENYP"
     aSDomain        5165..6337
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21160_AMP-binding.1"
                     /evalue="1.50E-109"
                     /label="CE053_RS21160_AMP-binding.1"
                     /locus_tag="CE053_RS21160"
                     /protein_end="865"
                     /protein_start="474"
                     /score="358.3"
                     /specificity="substrate consensus: Tyr"
                     /tool="antismash"
                     /translation="FEEQAHKTPEAAALKMGNECWTYRQLQVRANQIAHALIEKGVGSG
                     DIVAVMMGRSMEMPAALLGIWKAGGAYMPLDPHFPAERLSFLLKDSQAAQLLIEEDLIS
                     LIPPSYEGNTITIEHTESYQTEAPNMPPGDLAYLIYTSGTTGRPKGVLVDHHGIANTLQ
                     WRREEYGMTEQDISLHLFSYVFDGCVTSLFTPLLSGACVLLTTDDEAKDVLALKRKIAR
                     YKVSHMIIVPSLYRVLLEVMTADDAKSLRIVTFAGEAVTPDLLELSQMICPSAELANEY
                     GPTENSVATTILRHLNEKERITIGHPIRNTKVFVLHGNQMQPIGVAGELCISGAGLARG
                     YYKQQELTQKAFSDHPFLEGERLYRTGDAGRFLPDGTIEYIGRFDDQVKIR"
     CDS_motif       5351..5392
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0006"
                     /evalue="6.80E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="CE053_RS21160"
                     /protein_end="550"
                     /protein_start="536"
                     /score="16.1"
                     /tool="antismash"
                     /translation="LGIWKAGGAYMPLD"
     CDS_motif       5570..5629
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0007"
                     /evalue="1.60E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="CE053_RS21160"
                     /protein_end="629"
                     /protein_start="609"
                     /score="29.8"
                     /tool="antismash"
                     /translation="AYLIYTSGTTGRPKGVLVDH"
     CDS_motif       6002..6034
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0008"
                     /evalue="1.20E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="CE053_RS21160"
                     /protein_end="764"
                     /protein_start="753"
                     /score="5.7"
                     /tool="antismash"
                     /translation="EYGPTENSVAT"
     CDS_motif       6131..6220
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0009"
                     /evalue="1.70E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="CE053_RS21160"
                     /protein_end="826"
                     /protein_start="796"
                     /score="45.9"
                     /tool="antismash"
                     /translation="PIGVAGELCISGAGLARGYYKQQELTQKAF"
     CDS_motif       6302..6367
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0010"
                     /evalue="4.20E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="CE053_RS21160"
                     /protein_end="875"
                     /protein_start="853"
                     /score="34.8"
                     /tool="antismash"
                     /translation="YIGRFDDQVKIRGYRIELREIE"
     aSDomain        6653..6850
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21160_PCP.1"
                     /evalue="4.60E-23"
                     /label="CE053_RS21160_PCP.1"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1036"
                     /protein_start="970"
                     /score="73.2"
                     /tool="antismash"
                     /translation="EADLAHIWEDVLNKQHIGIRDDFFQLGGQSLKAAALVSRIHKKLN
                     VELPLSEVFSYPTVESMAVKL"
     aSDomain        6905..7765
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21160_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.30E-103"
                     /label="CE053_RS21160_Condensation_LCL.1"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1341"
                     /protein_start="1054"
                     /score="337.3"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="VYPLSFSQKRLYALHQLADDSTGYNMPAVLELRGSLNRQRLRSVL
                     TELVNRHEALRTVFVLDRDEPVQIIYPEIAFDLKELEMESEQMLESAIETFIKPFDLSS
                     GPLFRACVITMGNNRGFLLLDMHHIIADGVSMSTLVQEFTDLYCGKELPALNLHYKDFA
                     VWQQEKHPKELYKKQEAYWLGQLGGSLPTLELPIDKTRPRLPDFSGGTIEVNIDKDMAD
                     ELHRLMAETGTTLYMILLAVYSILLSKLSGQEDIVVGSPAAGRPHADLDRVIGMFVNTL
                     AMRSKP"
     aSModule        6905..11401
                     /complete
                     /domains="nrpspksdomains_CE053_RS21160_Condensation_LCL.1"
                     /domains="nrpspksdomains_CE053_RS21160_AMP-binding.2"
                     /domains="nrpspksdomains_CE053_RS21160_PCP.2"
                     /domains="nrpspksdomains_CE053_RS21160_Epimerization.1"
                     /domains="nrpspksdomains_CE053_RS21160_TIGR01720.1"
                     /locus_tags="CE053_RS21160"
                     /monomer_pairings="Thr -> D-Thr"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       6974..7084
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0011"
                     /evalue="1.30E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1114"
                     /protein_start="1077"
                     /score="52.9"
                     /tool="antismash"
                     /translation="YNMPAVLELRGSLNRQRLRSVLTELVNRHEALRTVFV"
     CDS_motif       7280..7345
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0012"
                     /evalue="1.90E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1201"
                     /protein_start="1179"
                     /score="30.0"
                     /tool="antismash"
                     /translation="MHHIIADGVSMSTLVQEFTDLY"
     CDS_motif       7376..7408
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0013"
                     /evalue="3.60E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1222"
                     /protein_start="1211"
                     /score="13.2"
                     /tool="antismash"
                     /translation="HYKDFAVWQQE"
     CDS_motif       7667..7756
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0014"
                     /evalue="6.60E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1338"
                     /protein_start="1308"
                     /score="50.6"
                     /tool="antismash"
                     /translation="DIVVGSPAAGRPHADLDRVIGMFVNTLAMR"
     CDS_motif       7811..7924
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0015"
                     /evalue="1.20E-14"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1394"
                     /protein_start="1356"
                     /score="46.6"
                     /tool="antismash"
                     /translation="LALTAYEHQDYPFEELADKLDTNREVNRNPLFDAMLVL"
     aSDomain        8258..9451
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21160_AMP-binding.2"
                     /evalue="1.60E-116"
                     /label="CE053_RS21160_AMP-binding.2"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1903"
                     /protein_start="1505"
                     /score="381.2"
                     /specificity="substrate consensus: Thr"
                     /tool="antismash"
                     /translation="FEKQAAKYPEHIAVVCGHSQLTYRDLNEKAERAAAMLIKQGVRTG
                     DIVGLMLDRSPDMVIGVLSILKAGGAYLPIDPEYPQERISFMLNDSGAKRLLTERGQNK
                     PADYNGHILYIDECENDSIPADVNIQEIVTDQPAYVIYTSGTTGQPKGVIVEHCNVISL
                     LKHQNLPFEFNHEDIWTLFHSYCFDFSVWEMFGALLNGGTLVVVSKETARDPHAFRLLL
                     KKERVTVLNQTPTAFYGLMLEDQNHTDHLNIRYVIFGGEALQPGLLQSWNEKYPHTDLI
                     NMYGITETTVHVTFKKLSAADIAKNKSNIGRPLSTLHAHVMDAHMNLQPIGVPGELYIG
                     GEGVARGYLNRDELTADRFVSNPYLPGDRLYRTGDLAKRLSNGELEYLGRIDEQVKVR"
     CDS_motif       8444..8485
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0016"
                     /evalue="1.70E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1581"
                     /protein_start="1567"
                     /score="15.0"
                     /tool="antismash"
                     /translation="LSILKAGGAYLPID"
     CDS_motif       8669..8728
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0017"
                     /evalue="4.50E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1662"
                     /protein_start="1642"
                     /score="31.4"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGQPKGVIVEH"
     CDS_motif       9107..9130
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0018"
                     /evalue="2.50E+00"
                     /label="NRPS-A_a5"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1796"
                     /protein_start="1788"
                     /score="2.0"
                     /tool="antismash"
                     /translation="YGITETTV"
     CDS_motif       9245..9334
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0019"
                     /evalue="3.10E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1864"
                     /protein_start="1834"
                     /score="57.8"
                     /tool="antismash"
                     /translation="PIGVPGELYIGGEGVARGYLNRDELTADRF"
     CDS_motif       9416..9481
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0020"
                     /evalue="3.10E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="CE053_RS21160"
                     /protein_end="1913"
                     /protein_start="1891"
                     /score="35.3"
                     /tool="antismash"
                     /translation="YLGRIDEQVKVRGHRIELGEIQ"
     aSDomain        9776..9976
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21160_PCP.2"
                     /evalue="2.20E-20"
                     /label="CE053_RS21160_PCP.2"
                     /locus_tag="CE053_RS21160"
                     /protein_end="2078"
                     /protein_start="2011"
                     /score="64.6"
                     /tool="antismash"
                     /translation="ETVMAEIWEDLLNVDELGVSANFFELGGDSIKALQVCARLKQRGF
                     ETTVREMFEHQTLGELSARVRK"
     aSDomain        10007..10918
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21160_Epimerization.1"
                     /evalue="4.70E-158"
                     /label="CE053_RS21160_Epimerization.1"
                     /locus_tag="CE053_RS21160"
                     /protein_end="2392"
                     /protein_start="2088"
                     /score="519.2"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDA"
                     /tool="antismash"
                     /translation="EGEITWTPIQQWFFSQSLESHHFNQSVMIYRTERFDEAALRKVLK
                     SLVTHHDALRIVCRHEDGRQVQINRGIDLSDEELYVLELFDVKDSLTEARNTIEEAASR
                     MQEHIRLETGPLLHAGLFRTENGDHLFLTIHHLVVDAVSWRILFEDFSAAYKQAVSGES
                     IKLPQKTDSYLTYSQRIADYSKSRQVQREAAYWDEYENRHIQPIPKDNDAASNTFKDTE
                     VIHFELSRHHTELLLTAAHKAYSTEMNDILLTALGLALQKWTGNNQFKISMEGHGRESY
                     LEDIDISRTVGWFTSIYPVWLDM"
     CDS_motif       10022..10048
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0021"
                     /evalue="2.90E-03"
                     /label="NRPS-E1"
                     /locus_tag="CE053_RS21160"
                     /protein_end="2102"
                     /protein_start="2093"
                     /score="10.8"
                     /tool="antismash"
                     /translation="WTPIQQWFF"
     CDS_motif       10106..10171
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0022"
                     /evalue="1.10E-03"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE053_RS21160"
                     /protein_end="2143"
                     /protein_start="2121"
                     /score="11.4"
                     /tool="antismash"
                     /translation="RFDEAALRKVLKSLVTHHDALR"
     CDS_motif       10394..10459
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0023"
                     /evalue="1.60E-11"
                     /label="NRPS-E2"
                     /locus_tag="CE053_RS21160"
                     /protein_end="2239"
                     /protein_start="2217"
                     /score="36.3"
                     /tool="antismash"
                     /translation="LFLTIHHLVVDAVSWRILFEDF"
     CDS_motif       10862..10912
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0024"
                     /evalue="5.10E-09"
                     /label="NRPS-E5"
                     /locus_tag="CE053_RS21160"
                     /protein_end="2390"
                     /protein_start="2373"
                     /score="28.0"
                     /tool="antismash"
                     /translation="DISRTVGWFTSIYPVWL"
     aSDomain        10952..11401
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21160_TIGR01720.1"
                     /evalue="1.80E-53"
                     /label="CE053_RS21160_TIGR01720.1"
                     /locus_tag="CE053_RS21160"
                     /protein_end="2553"
                     /protein_start="2403"
                     /score="172.5"
                     /tool="antismash"
                     /translation="LGHLIKQTKDMLHRIPHKGAGYGVLKYISKRWGSEKNSPEISFNY
                     LGQFDQDIQSKAFEVSDIKTGNEISPHWERPYILDISGAVSSGCLNMHIIYNRHQFEEE
                     TIQTFSRHFKQALDNIIEHCTGKENREWSASDFTDEDLTLDELSEI"
     CDS_motif       10973..11032
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21160_0025"
                     /evalue="4.90E-08"
                     /label="NRPS-E6"
                     /locus_tag="CE053_RS21160"
                     /protein_end="2430"
                     /protein_start="2410"
                     /score="25.3"
                     /tool="antismash"
                     /translation="TKDMLHRIPHKGAGYGVLKY"
     gene            11443..>14013
                     /locus_tag="CE053_RS21165"
     CDS             11443..>14013
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (2-30). E-value: 2e-16.
                     Score: 50.9. Matches aSDomain:
                     nrpspksdomains_CE053_RS21165_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (7-305). E-value:
                     6.6e-118. Score: 385.1. Matches aSDomain:
                     nrpspksdomains_CE053_RS21165_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (469-857). E-value:
                     7.4e-104. Score: 339.5. Matches aSDomain:
                     nrpspksdomains_CE053_RS21165_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389714.1"
                     /locus_tag="CE053_RS21165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_163182455.1"
                     /sec_met_domain="Condensation (E-value: 1.1e-93, bitscore:
                     301.3, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.1e-103, bitscore:
                     333.6, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQQPEIQDIYPLSFMQEGMLFHSLYDEQSRAYFEQASFTINGQL
                     DLERFQKSMDAVFDRYDIFRTAFIYKNVAKPRQVVLKQRHCHVHVEDISHLNEKDKEHC
                     TEAFKEQDKSKGFDLQTDVLMRISILKWAPDHYVCIWSHHHILMDGWCLGIVIKDFLHI
                     YQALGKGQFPDLPPVQPYGTYIKWLMQQEREEAAEYWKKRLQHFEKASPLPKRTDQMSD
                     GTLQQITFTIPEKETSELQKIAAACGATLNTVFQALWGIMLQKFNSLDDAVFGSVISGR
                     PSELKDVENMVGLFINTIPIRVQSDFLSFSDLVRSMQKDMTEAEAYSYFPLYDIQAQSA
                     LKQELIDHIIVFENTPTQQEIEELNQAGSFDFSVKDFEMEEVTNYRCSVKVIPGRTLYV
                     RIHFHTGTYQPNMMSEIKDYLQHMISDVISDPSLPVSKMTLLDEDKTRKIVSQNNRTVS
                     VSPEAPTLHGLFERQAAVTPERPAIRFSGGLLTYAELDLYASRLAAHLAARGVTNESIV
                     GVLSERSPDMLIAVLAVLKAGGAYLPLDPAYPKERLSYMLKDSGATLLLTQPGCSAPNF
                     SGEMLEVDMTSLASEKAENHLFTPADGGSLAYVIYTSGSTGQPKGVAVEHRQAVSFLTG
                     MQHQFPLSEDDIVMVKTSFSFDASVWQLFWWTLSGASAYLLPPGWEKDSALIVKAIHQE
                     KVTTAHFIPAMLNSFLDQAEMLALGDGTNLKRVFAGGEPLAPRTAARFASVLPQVSLIH
                     GYGPTEATVDAAFYVLDPERDRDRLRIPIGKPVPGARLYVLDPHLAVQPSGVAGELYIA
                     GAGVARGYLNRPALTEERFLEDPFYPGERMYKTGDVARWLPDGNV"
     aSDomain        11449..11532
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21165_NRPS-COM_Nterm.1"
                     /evalue="2.00E-16"
                     /label="CE053_RS21165_NRPS-COM_Nterm.1"
                     /locus_tag="CE053_RS21165"
                     /protein_end="30"
                     /protein_start="2"
                     /score="50.9"
                     /tool="antismash"
                     /translation="QQPEIQDIYPLSFMQEGMLFHSLYDEQS"
     aSDomain        11464..12357
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21165_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="6.60E-118"
                     /label="CE053_RS21165_Condensation_DCL.1"
                     /locus_tag="CE053_RS21165"
                     /protein_end="305"
                     /protein_start="7"
                     /score="385.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHILMDG"
                     /tool="antismash"
                     /translation="QDIYPLSFMQEGMLFHSLYDEQSRAYFEQASFTINGQLDLERFQK
                     SMDAVFDRYDIFRTAFIYKNVAKPRQVVLKQRHCHVHVEDISHLNEKDKEHCTEAFKEQ
                     DKSKGFDLQTDVLMRISILKWAPDHYVCIWSHHHILMDGWCLGIVIKDFLHIYQALGKG
                     QFPDLPPVQPYGTYIKWLMQQEREEAAEYWKKRLQHFEKASPLPKRTDQMSDGTLQQIT
                     FTIPEKETSELQKIAAACGATLNTVFQALWGIMLQKFNSLDDAVFGSVISGRPSELKDV
                     ENMVGLFINTIPIRVQS"
     aSModule        11464..>14013
                     /domains="nrpspksdomains_CE053_RS21165_Condensation_DCL.1"
                     /domains="nrpspksdomains_CE053_RS21165_AMP-binding.1"
                     /incomplete
                     /locus_tags="CE053_RS21165"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       11473..11514
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21165_0001"
                     /evalue="5.00E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="CE053_RS21165"
                     /protein_end="24"
                     /protein_start="10"
                     /score="15.2"
                     /tool="antismash"
                     /translation="YPLSFMQEGMLFHS"
     CDS_motif       11536..11652
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21165_0002"
                     /evalue="1.50E-17"
                     /label="C2_DCL_024-062"
                     /locus_tag="CE053_RS21165"
                     /protein_end="70"
                     /protein_start="31"
                     /score="55.8"
                     /tool="antismash"
                     /translation="AYFEQASFTINGQLDLERFQKSMDAVFDRYDIFRTAFIY"
     CDS_motif       11869..11934
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21165_0003"
                     /evalue="5.50E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="CE053_RS21165"
                     /protein_end="164"
                     /protein_start="142"
                     /score="34.9"
                     /tool="antismash"
                     /translation="HHHILMDGWCLGIVIKDFLHIY"
     CDS_motif       12253..12348
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21165_0004"
                     /evalue="7.80E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="CE053_RS21165"
                     /protein_end="302"
                     /protein_start="270"
                     /score="63.3"
                     /tool="antismash"
                     /translation="DAVFGSVISGRPSELKDVENMVGLFINTIPIR"
     CDS_motif       12394..12510
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21165_0005"
                     /evalue="1.00E-18"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="CE053_RS21165"
                     /protein_end="356"
                     /protein_start="317"
                     /score="59.8"
                     /tool="antismash"
                     /translation="QKDMTEAEAYSYFPLYDIQAQSALKQELIDHIIVFENTP"
     aSDomain        12850..>14013
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21165_AMP-binding.1"
                     /evalue="7.40E-104"
                     /label="CE053_RS21165_AMP-binding.1"
                     /locus_tag="CE053_RS21165"
                     /protein_end="857"
                     /protein_start="469"
                     /score="339.5"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="FERQAAVTPERPAIRFSGGLLTYAELDLYASRLAAHLAARGVTNE
                     SIVGVLSERSPDMLIAVLAVLKAGGAYLPLDPAYPKERLSYMLKDSGATLLLTQPGCSA
                     PNFSGEMLEVDMTSLASEKAENHLFTPADGGSLAYVIYTSGSTGQPKGVAVEHRQAVSF
                     LTGMQHQFPLSEDDIVMVKTSFSFDASVWQLFWWTLSGASAYLLPPGWEKDSALIVKAI
                     HQEKVTTAHFIPAMLNSFLDQAEMLALGDGTNLKRVFAGGEPLAPRTAARFASVLPQVS
                     LIHGYGPTEATVDAAFYVLDPERDRDRLRIPIGKPVPGARLYVLDPHLAVQPSGVAGEL
                     YIAGAGVARGYLNRPALTEERFLEDPFYPGERMYKTGDVARWLPDGNV"
     CDS_motif       13036..13077
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21165_0006"
                     /evalue="5.20E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="CE053_RS21165"
                     /protein_end="545"
                     /protein_start="531"
                     /score="19.5"
                     /tool="antismash"
                     /translation="LAVLKAGGAYLPLD"
     CDS_motif       13261..13320
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21165_0007"
                     /evalue="3.50E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="CE053_RS21165"
                     /protein_end="626"
                     /protein_start="606"
                     /score="34.8"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGQPKGVAVEH"
     CDS_motif       13846..13935
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21165_0008"
                     /evalue="5.00E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="CE053_RS21165"
                     /protein_end="831"
                     /protein_start="801"
                     /score="57.1"
                     /tool="antismash"
                     /translation="PSGVAGELYIAGAGVARGYLNRPALTEERF"
ORIGIN
        1 actcggcatg tttgtgaaca cgctggcgct tcggacgcgc ccggagggcg ggaagccgtt
       61 tgtacagtat ttgcaggaag tgcgtgagac agcattggaa gcctttgagc atcaggatta
      121 cccgttcgaa gagcttgtcg ataagcttga attgacacgg gatatgagcc ggaacccagt
      181 gtttgatgcg atgtttatcc ttcaaaatgt ggaaaaacaa gacatagatc tgagagaaat
      241 aaaagtccgg cctgccaatt tcgcacatca catatcttta tttgatatca cattaatagc
      301 tactgaaata aatggatcta tttgctgcga aatggaattt agtacagaag tattcctgaa
      361 agctacgatt gaacgctggg ccgatcattt taccgaattt ctgcatgcgg cactttcaac
      421 ccctgaaaca agccttgcac aaataaacat acttagtgat aaagaaaaac aaaaaatagt
      481 ctttgaattt aacaaaactc aagttgaatt tgctcaaaaa gatattcctt ttcatcgcat
      541 atttgaagcg aaagctgaag aaaattcaga acatatagct gttattgata acgaaacaga
      601 gattagctat cggcttctaa atgaaagagc caatcgtctg gcacgaacat tgcaaaacag
      661 aaaaggaccg aagccaactg tggctgtttt agccaagcgg tccatcgatg caattgttgg
      721 cgtgctggca gtgatgaaag ctggcggagt gtatatccca atcgattcac attatccaaa
      781 agctcgtatt gaatacattc ttcgtgatag cggggcagat attcttcttt tacagagaga
      841 gttgaagcat ttgatttcga actcgcctga atctgaaatg tctcacatct tccttgacga
      901 tgagggctct tttgaagaaa gtaactgcaa ccttaactta tctcctgctc ctgaagagcc
      961 ggtatatatc atttatacgt caggaacgac gggtgcgcct aaaggtgtta ttgtcaccta
     1021 tcagaacttt actcatgctg ctcttgcttg gcgtcaaatt tatgaactgg atcggaagcc
     1081 tgtcagactg cttcaaatcg cgagcttttc attcgatgtc ttctcaggag atttggcgag
     1141 aacattgacc aacggcggga ctctaatcgt ttgcccagat gaaacacggc tggaacctgc
     1201 tgaaatatat aaaatcatga actctcggag aatcacggtg atggagtcga caccggctct
     1261 cattatcccg gtgatggagt atgtataccg taatcagttc aagcttcctg acttagacat
     1321 actgattctt ggttccgaca tggtgaaagc tcaagatttc aaaacattga cagatcgctt
     1381 tggtcaaagc atgcgcatca ttaatagcta tggtgtcaca gaggcaacca ttgattcgag
     1441 cttctatgaa accagtatgg gtggagagtg tacgggagat aacgtaccaa tcggcagccc
     1501 gctgccgaat gtacacatgt acgtactcag ccagactgat caaattcagc cgatcggtgt
     1561 tgccggcgag ctgtgcatcg gcggggcagg tgttgcgaaa ggctatcatc ataaacctga
     1621 cttaacgcaa atgaaattta cagaaaatcc atttgtctct ggagaaaggc tgtacagaac
     1681 cggtgatcga gcatgctggc ttccaaacgg aacgatccgg cttcttggcc gaatggatta
     1741 tcaagtgaag ataaatggat acagaattga aacaaaagaa attgaaagtg ttctgcttca
     1801 aacggggctt gtcagagaag ctgctgttgc cgttcagcat gataagaatg gacaggctgg
     1861 tttggtagca tatattgtgc cttcagacgt caatacgaat gctctccgtg ctgctttaac
     1921 aaaggagctg ccagcgtata tgattcctgc acatctgatt cctttagaaa acatgccgct
     1981 aaccctaaac ggaaagctgg acagaaatgc cttgccggct ccaaataatg tgttatcgcg
     2041 tccatatacc gcaccagtta acgacataca aaaaacgatg gcgtatatat gggaagatgt
     2101 actgagcatg tcacgagtcg gcattcacga ttcctttttt gagcttggcg gagactcaat
     2161 taaagcatta caagtggcag cacgtctggc agctgaaggc tggagcatga caatacgcga
     2221 cttattccgt tattcaacga ttcaggaact atgcggacat atcacaccat tggcatctca
     2281 agcggaccag ggacctgcgg aaggtgaagc tgaactcacc ccgattcagc gaagattttt
     2341 cggacaggta catgcttttc actatcatta caaccagtcc gtcatgcttt tcagtgaaaa
     2401 aggattcaat gctaacgcgc ttcatctagc gctgcgtaaa ataaccgagc atcatgatgc
     2461 cattcgcatg attttccaac gtgaccaaaa cggccatgtc attcagttta atcgcggaat
     2521 caatcacaag gatcatgaac tgtttggcct ttatatttca gattggacaa aagcatcgct
     2581 ggaaagggct catcttgatg aaaaacttga cgctgaggaa accgtcattc aaagcaaaat
     2641 gaatgtagaa aaaggtcctt tgctgcaagc aggtttgttt aaaacggcag aaggggatca
     2701 tttgctgatt gcacttcatc atcttgtgat agatggcgtt tcttggagaa ttctgcttga
     2761 ggatttagct gcagcatatc aacaggcttt agaaaagaaa gaaatccaat taccgccaaa
     2821 aacggattct tatttatcct acgccgatgg tcttacccaa atagctgaaa gtaaacaatt
     2881 gctttcagag aaaccgtatt ggcaaacgat tttagacgct cacactgctt ttcttcctaa
     2941 ggacatagaa aatgtgccgg acagactcca aatgaacagc gacgcagctg cgtttgtact
     3001 atccggggac tggactgaaa aattgctgtt tgaaacccag caagcctatg gtactgatgc
     3061 caacgaactt ttgcttacgg ctttaggaat ggcgctttct gaatggactg ggcatgacca
     3121 aatcgtgatc agcactgagg gacacggcag agaagggcat gtgccaaaca ttgatatttc
     3181 aaggacagtt ggatggttta cttccattta tcctattctg ctagacatgg gtattcccga
     3241 gcctttcgag gatcagctcg cataccgaat taagacgaca aaagatatgc tgagaagagt
     3301 accgaacaaa ggaactggat acggattgtt aacacacata ggagaactgc ggcataaaga
     3361 gcctgaggtc agctttaatt atctcggcca atttagcgaa gagaaggaag ctgatacgtt
     3421 tgaattatca tattacaagc cccgttacga aatagcgggt gaaagagaac gagagtatga
     3481 gctggacatc aacgctctca ttacggacgg acggcttcaa gtaaaagcga tatacaccca
     3541 ggtgttcagc aaacactcaa ttgagtgctt tatggatcgt tttcacaggc atcttatcga
     3601 gacgattgag cattgttcgc agaaaaaagc acgcgaaaaa acattgagcg atttcagcaa
     3661 taaggaatta acgttgtctg cattatctag catcgaagat ctcgtgaaag atctttaact
     3721 tttgaaaagg tgtgtggaat tgatggcaca atcggcacaa attcaagata tttatccgtt
     3781 atcgcacatg caggaaggaa tgctgtttca ttcactaatg gatttcagct cgaaagcata
     3841 tatcgaacag acctcgttta cgatcacagg gaatctatgc gtcgactcgt ttcaaaagag
     3901 cttaaacctt cttgtttcta gatatgatat tttcagaacg attttcatta aagaagtacc
     3961 agatcttaca ggtccgcagc aggtagtctt atcaaaccgg gaattgacag tatatagaga
     4021 agatatttct cgcctggctg atcaagaaca gcaggcgctg attgatgctt ttatgactaa
     4081 ggatcgggaa aaaggatttg atttgcaaaa agatccttta atgcgtcttg ccctttttga
     4141 tagaggagac agccaatata catgtgtctg gacacatcac catatcatca tggatgggtg
     4201 gtgtctcggc attattctta aagagttttt cagcatgtat gattcgctca aaaataactc
     4261 acctgtacag cttggcagca cggtgccgta cagccgttat attgaatggc tcggagaaca
     4321 agatcaagaa gagactgctg cctactggag cgaatatttg aaggagtacg gcaatactgc
     4381 ttctattccc cgaataaggc gccgcacggc agagggggat tataaagccg cccagatcag
     4441 cttttcatta gcgccggata tggttgagaa actgacagag gctgcccaaa actggggagt
     4501 gacattaaac acgctgttta tgagtatttg gggcgtgctg ctccatcgat ataatgctgc
     4561 agatgatgcc gtttttggct ccgtcatttc gggacgcccg tcagcgattg acgggattga
     4621 atcaatggtc ggcttgttta tcaatactgt tccagtgcga atccgatctg cggaaggcat
     4681 aacgttttct tcgctgatca aagccgtaca ggaggatatt ttatcatctg aacagcatgg
     4741 ctattatccg ctttatgaga ttcaaaacca tagtccattg aagcaagggt tgatcgatca
     4801 tatttttgtt tttgaaaatt accctgtaca gctttatcag gcattaagcg tggagagtga
     4861 aaatgacgaa ggcgctctga agctgagtga catttcaatg tcggaacaaa ccaattacga
     4921 ctttaatatc gttatcgttc cgggagaatc attttatatc aaattcagct acaacgcaga
     4981 cgtttacgag cgggaggaga tgctgagaat tcaggggcat ctaaagcaag cactggattg
     5041 cattttgaca aatcccgatg ttgccgtcag tgacatcaat atcgtgccgc cggaggagca
     5101 acaggttatc caatcgttta acgaaactga gcgtccttat gtgaacaaaa caatacctca
     5161 attgttcgag gaacaagctc acaaaacacc tgaagctgct gcattgaaaa tgggaaacga
     5221 atgctggacc tatcgccagt tacaagtaag agccaatcag attgcgcacg ctctgataga
     5281 aaaaggcgtt ggatctggag atatcgttgc tgtaatgatg ggccggtcaa tggaaatgcc
     5341 tgcagctctg cttgggattt ggaaagcagg cggcgcttat atgccgcttg atccacattt
     5401 tccagcagaa cgtctttctt tcctgctgaa ggacagtcaa gcggctcaat tgctgataga
     5461 agaagacctt atttccttga tccctccgtc ctatgaagga aatacgataa cgatagaaca
     5521 tacagaaagt taccaaacag aagctccaaa tatgccgcca ggtgatctag cctacttgat
     5581 ctatacgtcg ggaacgacgg ggcgccctaa aggggtttta gttgatcatc acggtattgc
     5641 caatacattg caatggagac gggaagagta tggcatgacc gaacaggata tatccctcca
     5701 tttgttttcg tacgtgtttg acggctgtgt aacgagctta tttaccccgc ttttatctgg
     5761 tgcgtgcgta ctgctgacaa cagatgacga agcgaaggat gtgctggccc ttaagcgaaa
     5821 aatagctcgt tataaggtca gccacatgat cattgttcct tccctgtaca gggtgttatt
     5881 ggaagtgatg actgctgacg atgcaaaaag tcttcgtatt gtgacatttg cgggtgaagc
     5941 ggtcacgcct gatctgcttg agttgagtca aatgatttgt ccttctgcag aactggcaaa
     6001 tgaatacggg cccacagaaa acagtgtggc aacaacaata ttgcggcatc tgaatgaaaa
     6061 agagaggatt acgatcggac acccgatcag aaacacaaaa gtatttgttt tgcacggaaa
     6121 tcaaatgcag ccgatcggcg tggcgggtga actgtgtatt tccggcgcgg gtcttgcgag
     6181 aggatactac aaacagcaag agctgacaca gaaagcattc tctgatcatc cattccttga
     6241 aggggagcgt ttataccgaa caggtgatgc aggccgcttt ttgcctgatg gaacgattga
     6301 atatattgga cgttttgatg atcaagtaaa aattaggggt taccgtattg aactgagaga
     6361 gattgaaaca gttcttcgac aagcaccggg ggtaaaggaa gccgcggtac tggcccgtga
     6421 tgtttctgct gaggaaaagg agcttgttgc ttatatcgtt ccagaaaagg gaaacagcct
     6481 cccagacttg tatcagcatc ttgccgggac attgccgtcc tatatgatcc cagcaagtat
     6541 tattaacatc agctggatgc cattaacatc cagcggcaag cttgacaggt ttgcgcttcc
     6601 tgaaccagaa aacaatacga gtgtcacata tatggcgccc cgaaccttaa tcgaagctga
     6661 tctcgcacat atttgggaag atgtgctgaa taaacagcac atcggcattc gtgatgattt
     6721 ctttcagtta ggcggacagt ccttaaaagc ggccgcactg gtatcaagaa tacataaaaa
     6781 gcttaatgtc gagctgccgc tcagtgaggt gttttcttat ccgacggttg aaagcatggc
     6841 ggtcaaattg atgagcttaa aagaacacgc ttttacacag attgaacctg cggatcaaag
     6901 agatgtctat ccgctgtctt tctcgcaaaa acgattatat gctcttcatc agctggcaga
     6961 tgacagcaca ggttataaca tgccagctgt acttgaatta cgcggaagtc tgaatcgcca
     7021 gcggttgaga agcgttctca cagaacttgt gaatcggcat gaagcattac ggactgtttt
     7081 tgtgcttgac agagacgagc ctgtgcagat catttaccca gagatagctt ttgatctaaa
     7141 agagcttgaa atggaatcag aacaaatgct tgagtctgca atcgagacct ttatcaaacc
     7201 gtttgatcta tcatccggtc cgctttttag agcatgtgtc attacaatgg gcaataatcg
     7261 cggctttttg ctgctcgata tgcaccatat tattgccgac ggtgtctcga tgagcactct
     7321 tgtccaagaa tttactgact tatattgcgg aaaagagttg ccagctttaa acctgcatta
     7381 taaggatttt gctgtttggc agcaagagaa gcacccgaaa gagctataca aaaaacagga
     7441 ggcctactgg ctcggtcagc ttggcgggag tcttcctaca ctagaattac cgattgataa
     7501 aacaaggccg cgtctccctg atttcagcgg tggaacaatt gaagtcaaca ttgataaaga
     7561 catggctgat gaactgcacc gtttaatggc tgaaacggga acaacattgt atatgattct
     7621 tctggctgtc tattctattt tgttatctaa gctaagcggg caagaggata ttgttgtcgg
     7681 ttcgcctgct gccggaagac cgcacgctga tctggatcgc gtcatcggga tgttcgtcaa
     7741 tacattggcg atgcgcagca agcccgaggg ccacaaaacg ttcagctcct atttgcagga
     7801 cattcgccac ctggctctga ccgcttacga gcatcaggat tatccttttg aggagctggc
     7861 agataagctc gatacaaatc gagaggtaaa tcgcaacccg ctgttcgatg cgatgcttgt
     7921 gcttcaaagc agtgaagatt tccggttcga agtacctggt ctgtcgattt cgtctgtcac
     7981 tcctaaacat gatatatcta aattcgattt aacattacac gctgaggagc atccgagtgg
     8041 aatacgctgc cgttttgaat acagcactgc tctttttgaa gaagaaacca ttacgcagtg
     8101 ggcatcctat tttattgaat tggtgaaagg ggtaacggcg gataccgaga tgagaatttc
     8161 ggacatgcaa ttgcttcctg ctgcagagcg taggttgctc cttgaaaaaa tgggacagta
     8221 tgcggcttat ccgagaaatg aaaacatagt aagcttattt gaaaagcaag ccgctaaata
     8281 tccggagcat atcgcagtgg tgtgcggcca ttctcagctt acgtatcgag acctgaatga
     8341 aaaagccgag cgggcagctg ctatgctgat caaacaaggg gtgagaactg gcgatatcgt
     8401 cgggctgatg ctcgatcggt cacccgatat ggtcattggc gttttatcga ttttaaaagc
     8461 tggaggggcg tacttgccga ttgatcctga atatcctcag gagcgtatta gctttatgct
     8521 caatgacagc ggggcaaaac ggctgctgac agagcgtggt caaaacaaac ctgctgacta
     8581 caatgggcat attttgtata tcgatgaatg tgaaaacgac tcaattccgg ctgatgtaaa
     8641 cattcaagaa atagtaacag atcagcccgc atacgtgata tacacatctg gaacgactgg
     8701 ccagccaaag ggagtcattg tcgagcactg caatgtgatc agtctcttga agcatcaaaa
     8761 cctgcctttt gaatttaacc atgaagacat ctggacgtta tttcattctt attgctttga
     8821 tttctctgta tgggagatgt tcggtgcgtt attgaacgga ggcacattag tcgtggtctc
     8881 taaagaaaca gcccgtgacc cccatgcatt ccgtttattg ttaaaaaaag agcgcgtcac
     8941 tgtactcaat cagaccccga ctgcttttta cggcttgatg cttgaagacc aaaatcatac
     9001 agatcattta aacatccgct atgtgatttt tggcggcgaa gccttacagc ccggattgct
     9061 tcaaagctgg aatgaaaaat atcctcatac tgatttgatt aatatgtacg gtattacaga
     9121 aacaacggtc catgtaacat tcaaaaaatt gtccgcagca gacattgcaa aaaataaaag
     9181 caatatcgga cggccgcttt ctactctgca tgctcatgtg atggatgccc atatgaacct
     9241 gcagccgatc ggagttccag gggagctgta tataggcggc gaaggtgtgg cacggggata
     9301 tctcaacaga gatgagctga ccgctgaccg ttttgtatcc aatccttatc ttccgggaga
     9361 cagattatac cgaacaggag atcttgctaa gcgcctgtca aatggcgagc tggaatactt
     9421 aggccgcatt gatgagcagg tgaaagtcag agggcaccgt attgagttag gcgaaatcca
     9481 agcggcactt cttcagtatc caatgattaa agaggcggct gtcattacca gagcagacga
     9541 gcgagggcaa acagctatat atgcatatat ggtgataaaa gatcaacagg ctgcgaatat
     9601 atcagacatc cgggcttccc taaaaaacgc cttgcctgac tttatgctgc ctgcccgtat
     9661 gatacaaatt gacagcatcc cggtgactgt taatggaaag ctcgatcaga aagccctgcc
     9721 agagcctgaa aaacacgctt atacagctga tgatatcagt cctagaaatg agattgaaac
     9781 ggtgatggct gaaatttggg aggatcttct taatgtagat gagctgggtg taagcgccaa
     9841 tttctttgag cttggcggag actctattaa agcgctgcag gtttgtgcca gactgaagca
     9901 gcgcggattt gaaacgacag tacgtgagat gtttgaacat cagactcttg gtgaactgtc
     9961 cgcccgggtt cgaaaagacg tacgcgccat tgaccaaggc ccggtcgaag gcgagattac
    10021 ttggacacct atccagcagt ggtttttctc gcaatctctg gaaagccatc attttaacca
    10081 atccgttatg atctaccgga ctgaacgatt tgatgaggct gcgcttagaa aagtactaaa
    10141 aagccttgtg actcatcatg atgcactgag aatcgtatgc cggcatgaag atggcaggca
    10201 agtgcagata aacagaggga tagatctttc agatgaggag ctatatgtac ttgaactgtt
    10261 tgatgtaaag gatagcctaa cagaagcccg gaacacgatt gaggaagcag ccagccggat
    10321 gcaagaacat attcgtttgg aaaccggccc cttacttcac gctggattgt ttagaaccga
    10381 gaatggtgac catttatttc tgacgattca tcacttagtc gttgatgcgg tttcttggcg
    10441 cattttgttt gaggattttt cagcggctta caaacaggca gtctcaggag aatctattaa
    10501 actgccgcaa aaaacagatt catatttaac gtattcacaa agaatagctg actactctaa
    10561 aagccgccag gtgcagcgcg aagcagcgta ctgggatgag tacgagaacc gtcacatcca
    10621 gcctattcca aaagacaatg acgcagcatc taacacattc aaagatacag aagtgattca
    10681 ttttgagcta tctcgccatc acacagaatt actattaacg gctgcacata aagcttacag
    10741 cacggagatg aatgatatcc ttctgacagc tttaggtctt gctttgcaga aatggacggg
    10801 aaataaccaa tttaaaatca gcatggaggg ccatggaagg gaatcatatc ttgaggacat
    10861 tgatattagc agaactgttg gctggttcac ctccatttat cctgtgtggc ttgatatgag
    10921 agattccgaa cttaaggaca aagaagagcg gctgggccac cttattaaac agacaaaaga
    10981 tatgctgcat cgtataccgc ataaaggcgc gggctacggc gtgctgaagt atatcagcaa
    11041 aagatggggc tctgagaaaa acagcccaga aatcagcttt aattatttag gccagtttga
    11101 tcaggatatt caatcaaaag cctttgaggt ttctgacatc aagacaggaa atgagatcag
    11161 cccgcactgg gagcgtcctt atatactgga tatcagcggc gccgtttctt cggggtgcct
    11221 gaacatgcac atcatctata acaggcatca atttgaggaa gaaaccattc aaacattttc
    11281 cagacatttt aaacaagctc tggataacat cattgagcat tgtaccggca aagaaaaccg
    11341 agaatggagc gcatctgatt tcactgatga agacttaacg cttgatgaat tgagtgagat
    11401 tatgggagcc gtcaacaaac tatagggaga ggagattcac tgatgccgca gcaacccgaa
    11461 atacaggata tatacccttt gtcatttatg caggaaggga tgctttttca ctcattgtat
    11521 gatgagcagt cgagagctta ttttgaacag gcttctttta cgatcaatgg acagcttgac
    11581 ttggagcgtt tccagaaaag tatggatgct gtttttgaca ggtacgatat ctttcgaaca
    11641 gcattcatct ataaaaatgt agccaaacct cgtcaagtcg tgctcaagca acgtcattgc
    11701 catgttcatg ttgaagatat ttctcatctc aatgaaaaag ataaagaaca ttgtacagag
    11761 gcattcaaag aacaggacaa atcaaaaggc tttgatctcc aaacggatgt actgatgaga
    11821 atatctattt taaaatgggc gcctgatcat tatgtctgta tctggagcca ccatcatatt
    11881 ttaatggatg gctggtgttt aggcatcgtc attaaagact ttctccatat ttatcaagcg
    11941 cttggaaaag ggcaatttcc tgatttgcct cccgtacagc cgtacgggac gtatattaaa
    12001 tggctgatgc agcaagagcg tgaagaagca gctgagtatt ggaaaaaaag gcttcagcat
    12061 ttcgaaaaag cttcgcctct gccaaaaaga acagatcaaa tgtcggacgg gacattacag
    12121 cagatcacgt ttaccattcc tgagaaagaa acctccgaat tacagaaaat cgctgcagct
    12181 tgtggtgcga cgcttaacac tgttttccaa gctttatggg gaatcatgct ccaaaagttc
    12241 aatagcctcg acgatgctgt gttcggatca gttatatcag gcagaccttc tgagctaaaa
    12301 gatgtagaaa acatggtcgg actatttatc aataccattc ctattcgagt ccaaagtgat
    12361 tttctgtctt tttctgacct agtcagaagt atgcaaaagg acatgactga agcagaagcg
    12421 tatagttact tccctttata tgacattcag gcgcagagcg ctcttaaaca agagctgatt
    12481 gatcatatta tcgtctttga aaatacacct actcaacaag agattgaaga gctgaatcaa
    12541 gctggatcat ttgatttctc cgtgaaagat tttgagatgg aagaggtaac taattataga
    12601 tgcagtgtca aagtgattcc agggcgtact ttgtacgttc ggattcattt tcatacaggc
    12661 acttatcaac caaatatgat gtccgaaata aaagattatt tacagcacat gatctctgac
    12721 gtcatctctg atccatctct tcctgtgtca aagatgacat tgctagatga ggacaaaaca
    12781 cggaaaatcg tttctcaaaa taacaggaca gtatctgttt cacctgaagc cccaaccctg
    12841 catgggctgt ttgagcgaca ggcggcagtc acaccggagc ggccagcgat tcgcttttcc
    12901 ggcggtttgc tgacgtacgc tgagcttgac ttgtatgcca gtcggctggc tgcgcacctt
    12961 gcggcacgcg gcgtcacaaa cgaaagtatc gtcggtgtcc tgtctgagcg gtcacctgac
    13021 atgctgatcg ctgtccttgc tgtcttgaag gccggagggg catatttgcc gcttgatcct
    13081 gcgtatccaa aggagcggct gagctacatg ctgaaagaca gcggggcaac gctcttgctg
    13141 acacagccgg gatgctccgc accgaacttt tctggcgaaa tgcttgaagt ggacatgaca
    13201 tctcttgcga gcgaaaaagc agaaaaccat ctgtttactc ctgctgacgg cggttctctc
    13261 gcctatgtca tctatacatc gggctctacc gggcagccga aaggagtggc cgttgagcac
    13321 cgtcaggctg tttcctttct gaccggcatg cagcaccagt ttccgctttc ggaagatgac
    13381 attgtcatgg tgaaaacctc tttttccttt gatgcgtctg tctggcagct gttttggtgg
    13441 actctttccg gtgcttcggc gtatctgctt ccgcccggct gggaaaaaga ctccgcattg
    13501 atcgtaaaag ccattcatca ggaaaaagtg acaacggctc attttattcc ggctatgctg
    13561 aatagctttc tggatcaagc agaaatgttg gcgcttggtg acgggacaaa cctaaagcgt
    13621 gtattcgccg gaggtgaacc gcttgcgccc cgtacggcag cccgttttgc ttctgtattg
    13681 ccgcaggtat cattaatcca tggctacggg ccgacagaag caacggtaga cgcggcattt
    13741 tacgtattgg atccagaacg ggacagagac cgtttgcgga ttccgattgg gaagcccgta
    13801 cctggtgcgc gtttgtatgt tttagatccg catttagccg tacagccttc cggtgtcgcc
    13861 ggcgaactat acattgccgg agcgggtgtt gccagaggtt atttgaatcg gccggcatta
    13921 acggaggagc gttttcttga ggacccattt tacccgggtg aacgtatgta caaaacagga
    13981 gatgtggcgc gctggctccc tgacgggaat gtt
//
