LOCUS       NZ_NJRD01000014         9485 bp    DNA     linear   CON 22-JUN-2024
DEFINITION  Bacillus subtilis strain 20-3 20-3_R1_(paired)_contig_14, whole
            genome shotgun sequence.
ACCESSION   NZ_NJRD01000014
VERSION     NZ_NJRD01000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Cui,Y.
  TITLE     Improper usage of probiotics constitutes a potential risk of public
            health
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Cui,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2017) Beijing Advanced Innovation Center for Food
            Nutrition and Human Health, College of Veterinary Medicine, China
            Agricultural University, Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 9.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010578135.1-RS_2024_06_22
            Annotation Date                   :: 06/22/2024 02:42:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,274
            CDSs (total)                      :: 4,198
            Genes (coding)                    :: 4,064
            CDSs (with protein)               :: 4,064
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 80 of 134
            Pseudo Genes (incomplete)         :: 67 of 134
            Pseudo Genes (internal stop)      :: 22 of 134
            Pseudo Genes (multiple problems)  :: 31 of 134
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:13:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9485
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NJRD01000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9485
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>9483](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>9483)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..5778)
                     /gene="ppsD"
                     /locus_tag="CE053_RS21115"
                     /pseudo=""
     CDS             complement(<1..5778)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-30). E-value: 1.8e-18.
                     Score: 57.4. Matches aSDomain:
                     nrpspksdomains_CE053_RS21115_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (7-305). E-value:
                     8.6e-119. Score: 388.0. Matches aSDomain:
                     nrpspksdomains_CE053_RS21115_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (469-866). E-value:
                     4.5e-105. Score: 343.5. Matches aSDomain:
                     nrpspksdomains_CE053_RS21115_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (972-1041). E-value: 1.3e-23. Score:
                     75.0. Matches aSDomain: nrpspksdomains_CE053_RS21115_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1055-1334). E-value:
                     2.1e-103. Score: 337.4. Matches aSDomain:
                     nrpspksdomains_CE053_RS21115_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1500-1901). E-value:
                     5.2e-113. Score: 369.7. Matches aSDomain:
                     nrpspksdomains_CE053_RS21115_AMP-binding.2"
                     /codon_start=1
                     /gene="ppsD"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389713.2"
                     /locus_tag="CE053_RS21115"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="plipastatin non-ribosomal peptide synthetase
                     PpsD"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1.1e-89, bitscore:
                     287.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7e-17, bitscore:
                     49.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.4e-122, bitscore:
                     395.0, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLQKDSQAYVEQASFTIEGKV
                     NPQFFQNSINALVERHDIFRTIFISQNVSSPQQVVLRERNIIVLEEDITHLNEAEQSQF
                     IEQWKEKDRDRGFHLQKDVLMRIALIQTGESQYSCIWTFHHIMMDGWCLSIVLKEFLHI
                     YASYVNASPITLEPVQPYGKYIKWLMEQDKEQAVSYWDHYLSGHEQQTVLPKQKKKTKG
                     RSRQEHVTFSFSKEESSRLSELAAREEVTLSTIFHTIWGILLQKYNNNDDAVFGSVISG
                     RPAEIEGIEHMVGLFINTMPVRVQGAKTPFLQLIKDMQKDRLAAEAYSYHPLYEIQSRS
                     AVKQGLIDHILVFENYPVQQEIQMLNKQEHASDLFQIHNFTVADETNYSFYLMVAPGEE
                     IHIKMNYDAEQHDRSFVLSVKEHLLNAVSQILNNPNLPPEEIDITTDTEKRQLIGEITD
                     QTPVYETIHAMFEKQAEKTPDAHAVIDQACSLTYRELNKAANRLARHLRMKGVVRQEPV
                     AIMMERSAAFITGVLGILKAGGAIVPVDPHYPADRIRYILHDCGCSHVVSQAHLSSSLE
                     DNYIITHPEDIESKVDGSNIKSVNNADDLLYMIYTSGTTGKPKGVQFEHRNMANLLKFE
                     YTHSGIDFEADVLQFATPSFDVCYQEIFSALLKGGTLHIVPEAIKRDVPQLFAFINKHQ
                     TNIVFLPTAFIKMIFSERELANSFPDGVKHLIAAGEQLMISDLFQDVLRKRGIHLHNHY
                     GPSETHVVSTYTIHPGDPIPELPPIGKPIGCTDLYILNHQKQLQPCGVPGELYISGASV
                     ARGYVNHDKLTSDKFSSDPFKPDVIMYRTGDLARRLEDGNIEYIGRADNQVKIRGYRIE
                     PQEIEVTLMNHPDISEAAILIWQDQNGEHELCAYYCSVQKLNAIDLRSYMASELPEYMI
                     PAKWIWVDSIPLTPNGKVDRAALPEPDASISGNPYTAPRNLLEAKLSQLFEDVLNNGHI
                     GIQDNFFDHGGHSLKATVLMSRIAKEFHVQVPLKDIFAHPTVEGLASIIREAEQNPYAA
                     IEPAEKRDTYPVSSAQKRIYVLQQLDEGVAYNMPAVLELEGALDVAKLSAVCKELISRH
                     EPLRTSFVSGADDEPVQRIHTEVPFTLSKETTIEGFVRPFDLSQAPLFRAGLIEVSNEK
                     HVLLVDMHHIISDGVSVQLLIREFTDLYANRQLKPLRIQYKDYAVWQQKFKKGDSYQKQ
                     ETYWQQQFSGDLPILELPTDKRRPAERQFIGGKVTFQLDKEITARIKRLAHKNRSTLYM
                     TLLALYSAFLSRLSGQDDIVIGSPIAGRPHADLEAVLGMFVNTLALRTRPAGNKTFEEF
                     LKEVRQTALEAYEHQDYPFEELVDKLGVQREMSRNPLFDTTLVLQNMEQQKLKMNDVQL
                     QWNDLVHPISKFDISLYVTEHDSELFCQFEYSTALFEKETIQRWASLFTTLVEHTAASP
                     ETELDNIPILTKEEERDFIESCHLFEETGYSMNQTLHYALEQQAAKTPDQAAVIFEDGV
                     MTYKELNEQANRIAWELIGRGVKPETTVAIIGKRSPEMLLGIYGILKAGGAYLPIDPDY
                     PEERISFLLEDSGTNILLLQSAGLHVPEFTGEIVYLNQKNSGLAHRLSNPNVDVLPQSL
                     AYVIYTSGSTGMPKGVEIEHRSAVNFLNSLQSRYQLKHSDMIMHKTSYSFDASIWELFW
                     WPYAGASVYLLPQGGEKEPEVIVKAIEEQKITAMHFVPSMLHAFLEHIKYRSVPIKTNR
                     LKRVFSGGEQLGTHLVSRFHELLPNVSITNSYGPTEATVEAAFFDCPLHEKLERIPIGK
                     PVHHVRLYILNQNQRMLPAGCIGELYIAGAGVARGYLNRPALTEERFLEDPFYPGERMY
                     KTGDVARWLPDGNVEFLGRTDDQVKIRGYRIEPGEIEAALRSIEGVREAAVT"
     source          1..9485
                     /collection_date="2016-05"
                     /db_xref="taxon:1423"
                     /geo_loc_name="China"
                     /mol_type="genomic DNA"
                     /note="commercial probiotic used for animal"
                     /organism="Bacillus subtilis"
                     /strain="20-3"
                     /submitter_seqid="20-3_R1_(paired)_contig_14"
     region          1..9485
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9485
                     /SMILES="NC(C(C)C)C(=O)N1C(CCC1)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(46..111)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0021"
                     /evalue="1.10E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1911"
                     /protein_start="1889"
                     /score="39.7"
                     /tool="antismash"
                     /translation="FLGRTDDQVKIRGYRIEPGEIE"
     aSDomain        complement(76..1278)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21115_AMP-binding.2"
                     /evalue="5.20E-113"
                     /label="CE053_RS21115_AMP-binding.2"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1901"
                     /protein_start="1500"
                     /score="369.7"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="LEQQAAKTPDQAAVIFEDGVMTYKELNEQANRIAWELIGRGVKPE
                     TTVAIIGKRSPEMLLGIYGILKAGGAYLPIDPDYPEERISFLLEDSGTNILLLQSAGLH
                     VPEFTGEIVYLNQKNSGLAHRLSNPNVDVLPQSLAYVIYTSGSTGMPKGVEIEHRSAVN
                     FLNSLQSRYQLKHSDMIMHKTSYSFDASIWELFWWPYAGASVYLLPQGGEKEPEVIVKA
                     IEEQKITAMHFVPSMLHAFLEHIKYRSVPIKTNRLKRVFSGGEQLGTHLVSRFHELLPN
                     VSITNSYGPTEATVEAAFFDCPLHEKLERIPIGKPVHHVRLYILNQNQRMLPAGCIGEL
                     YIAGAGVARGYLNRPALTEERFLEDPFYPGERMYKTGDVARWLPDGNVEFLGRTDDQVK
                     IR"
     aSModule        complement(76..2613)
                     /domains="nrpspksdomains_CE053_RS21115_Condensation_LCL.1"
                     /domains="nrpspksdomains_CE053_RS21115_AMP-binding.2"
                     /incomplete
                     /locus_tags="CE053_RS21115"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(193..282)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0020"
                     /evalue="5.40E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1862"
                     /protein_start="1832"
                     /score="57.0"
                     /tool="antismash"
                     /translation="PAGCIGELYIAGAGVARGYLNRPALTEERF"
     CDS_motif       complement(391..420)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0019"
                     /evalue="4.10E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1796"
                     /protein_start="1786"
                     /score="7.0"
                     /tool="antismash"
                     /translation="SYGPTEATVE"
     CDS_motif       complement(805..864)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0018"
                     /evalue="9.40E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1658"
                     /protein_start="1638"
                     /score="30.4"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGMPKGVEIEH"
     CDS_motif       complement(1051..1089)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0017"
                     /evalue="8.10E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1576"
                     /protein_start="1563"
                     /score="15.9"
                     /tool="antismash"
                     /translation="GILKAGGAYLPID"
     CDS_motif       complement(1618..1737)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0016"
                     /evalue="6.30E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1387"
                     /protein_start="1347"
                     /score="66.8"
                     /tool="antismash"
                     /translation="RQTALEAYEHQDYPFEELVDKLGVQREMSRNPLFDTTLVL"
     aSDomain        complement(1777..2613)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21115_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.10E-103"
                     /label="CE053_RS21115_Condensation_LCL.1"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1334"
                     /protein_start="1055"
                     /score="337.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="DTYPVSSAQKRIYVLQQLDEGVAYNMPAVLELEGALDVAKLSAVC
                     KELISRHEPLRTSFVSGADDEPVQRIHTEVPFTLSKETTIEGFVRPFDLSQAPLFRAGL
                     IEVSNEKHVLLVDMHHIISDGVSVQLLIREFTDLYANRQLKPLRIQYKDYAVWQQKFKK
                     GDSYQKQETYWQQQFSGDLPILELPTDKRRPAERQFIGGKVTFQLDKEITARIKRLAHK
                     NRSTLYMTLLALYSAFLSRLSGQDDIVIGSPIAGRPHADLEAVLGMFVNTLALRTRP"
     CDS_motif       complement(1786..1875)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0015"
                     /evalue="2.60E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1331"
                     /protein_start="1301"
                     /score="51.9"
                     /tool="antismash"
                     /translation="DIVIGSPIAGRPHADLEAVLGMFVNTLALR"
     CDS_motif       complement(2134..2166)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0014"
                     /evalue="3.20E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1215"
                     /protein_start="1204"
                     /score="13.4"
                     /tool="antismash"
                     /translation="QYKDYAVWQQK"
     CDS_motif       complement(2197..2262)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0013"
                     /evalue="1.70E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1194"
                     /protein_start="1172"
                     /score="30.1"
                     /tool="antismash"
                     /translation="MHHIISDGVSVQLLIREFTDLY"
     CDS_motif       complement(2434..2547)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0012"
                     /evalue="6.80E-15"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1115"
                     /protein_start="1077"
                     /score="47.4"
                     /tool="antismash"
                     /translation="AYNMPAVLELEGALDVAKLSAVCKELISRHEPLRTSFV"
     aSDomain        complement(2656..2862)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21115_PCP.1"
                     /evalue="1.30E-23"
                     /label="CE053_RS21115_PCP.1"
                     /locus_tag="CE053_RS21115"
                     /protein_end="1041"
                     /protein_start="972"
                     /score="75.0"
                     /tool="antismash"
                     /translation="EAKLSQLFEDVLNNGHIGIQDNFFDHGGHSLKATVLMSRIAKEFH
                     VQVPLKDIFAHPTVEGLASIIREA"
     aSModule        complement(2656..5757)
                     /complete
                     /domains="nrpspksdomains_CE053_RS21115_Condensation_DCL.1"
                     /domains="nrpspksdomains_CE053_RS21115_AMP-binding.1"
                     /domains="nrpspksdomains_CE053_RS21115_PCP.1"
                     /locus_tags="CE053_RS21115"
                     /monomer_pairings="Pro -> Pro"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(3151..3216)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0011"
                     /evalue="6.30E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CE053_RS21115"
                     /protein_end="876"
                     /protein_start="854"
                     /score="37.4"
                     /tool="antismash"
                     /translation="YIGRADNQVKIRGYRIEPQEIE"
     aSDomain        complement(3181..4371)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21115_AMP-binding.1"
                     /evalue="4.50E-105"
                     /label="CE053_RS21115_AMP-binding.1"
                     /locus_tag="CE053_RS21115"
                     /protein_end="866"
                     /protein_start="469"
                     /score="343.5"
                     /specificity="substrate consensus: Pro"
                     /tool="antismash"
                     /translation="FEKQAEKTPDAHAVIDQACSLTYRELNKAANRLARHLRMKGVVRQ
                     EPVAIMMERSAAFITGVLGILKAGGAIVPVDPHYPADRIRYILHDCGCSHVVSQAHLSS
                     SLEDNYIITHPEDIESKVDGSNIKSVNNADDLLYMIYTSGTTGKPKGVQFEHRNMANLL
                     KFEYTHSGIDFEADVLQFATPSFDVCYQEIFSALLKGGTLHIVPEAIKRDVPQLFAFIN
                     KHQTNIVFLPTAFIKMIFSERELANSFPDGVKHLIAAGEQLMISDLFQDVLRKRGIHLH
                     NHYGPSETHVVSTYTIHPGDPIPELPPIGKPIGCTDLYILNHQKQLQPCGVPGELYISG
                     ASVARGYVNHDKLTSDKFSSDPFKPDVIMYRTGDLARRLEDGNIEYIGRADNQVKIR"
     CDS_motif       complement(3298..3387)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0010"
                     /evalue="6.50E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="CE053_RS21115"
                     /protein_end="827"
                     /protein_start="797"
                     /score="40.9"
                     /tool="antismash"
                     /translation="PCGVPGELYISGASVARGYVNHDKLTSDKF"
     CDS_motif       complement(3496..3522)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0009"
                     /evalue="4.30E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="CE053_RS21115"
                     /protein_end="761"
                     /protein_start="752"
                     /score="4.2"
                     /tool="antismash"
                     /translation="YGPSETHVV"
     CDS_motif       complement(3904..3960)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0008"
                     /evalue="1.40E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="CE053_RS21115"
                     /protein_end="625"
                     /protein_start="606"
                     /score="26.9"
                     /tool="antismash"
                     /translation="YMIYTSGTTGKPKGVQFEH"
     CDS_motif       complement(4144..4185)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0007"
                     /evalue="5.30E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="CE053_RS21115"
                     /protein_end="545"
                     /protein_start="531"
                     /score="13.5"
                     /tool="antismash"
                     /translation="LGILKAGGAIVPVD"
     CDS_motif       complement(4708..4824)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0006"
                     /evalue="1.90E-20"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="CE053_RS21115"
                     /protein_end="357"
                     /protein_start="318"
                     /score="65.4"
                     /tool="antismash"
                     /translation="QKDRLAAEAYSYHPLYEIQSRSAVKQGLIDHILVFENYP"
     aSDomain        complement(4864..5757)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21115_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="8.60E-119"
                     /label="CE053_RS21115_Condensation_DCL.1"
                     /locus_tag="CE053_RS21115"
                     /protein_end="305"
                     /protein_start="7"
                     /score="388.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QDIYPLSYMQEGMLFHSLLQKDSQAYVEQASFTIEGKVNPQFFQN
                     SINALVERHDIFRTIFISQNVSSPQQVVLRERNIIVLEEDITHLNEAEQSQFIEQWKEK
                     DRDRGFHLQKDVLMRIALIQTGESQYSCIWTFHHIMMDGWCLSIVLKEFLHIYASYVNA
                     SPITLEPVQPYGKYIKWLMEQDKEQAVSYWDHYLSGHEQQTVLPKQKKKTKGRSRQEHV
                     TFSFSKEESSRLSELAAREEVTLSTIFHTIWGILLQKYNNNDDAVFGSVISGRPAEIEG
                     IEHMVGLFINTMPVRVQ"
     CDS_motif       complement(4870..4965)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0005"
                     /evalue="1.00E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="CE053_RS21115"
                     /protein_end="303"
                     /protein_start="271"
                     /score="62.9"
                     /tool="antismash"
                     /translation="DAVFGSVISGRPAEIEGIEHMVGLFINTMPVR"
     CDS_motif       complement(5203..5241)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0004"
                     /evalue="1.20E-02"
                     /label="C4_DCL_171-183"
                     /locus_tag="CE053_RS21115"
                     /protein_end="192"
                     /protein_start="179"
                     /score="8.7"
                     /tool="antismash"
                     /translation="PYGKYIKWLMEQD"
     CDS_motif       complement(5287..5352)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0003"
                     /evalue="5.10E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="CE053_RS21115"
                     /protein_end="164"
                     /protein_start="142"
                     /score="35.0"
                     /tool="antismash"
                     /translation="FHHIMMDGWCLSIVLKEFLHIY"
     CDS_motif       complement(5572..5685)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0002"
                     /evalue="1.30E-15"
                     /label="C2_DCL_024-062"
                     /locus_tag="CE053_RS21115"
                     /protein_end="69"
                     /protein_start="31"
                     /score="49.7"
                     /tool="antismash"
                     /translation="AYVEQASFTIEGKVNPQFFQNSINALVERHDIFRTIFI"
     aSDomain        complement(5689..5778)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21115_NRPS-COM_Nterm.1"
                     /evalue="1.80E-18"
                     /label="CE053_RS21115_NRPS-COM_Nterm.1"
                     /locus_tag="CE053_RS21115"
                     /protein_end="30"
                     /protein_start="0"
                     /score="57.4"
                     /tool="antismash"
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLQKDS"
     CDS_motif       complement(5707..5748)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21115_0001"
                     /evalue="8.90E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="CE053_RS21115"
                     /protein_end="24"
                     /protein_start="10"
                     /score="14.5"
                     /tool="antismash"
                     /translation="YPLSYMQEGMLFHS"
     CDS             complement(5804..>9483)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (181-574). E-value:
                     3.4e-115. Score: 376.8. Matches aSDomain:
                     nrpspksdomains_CE053_RS21120_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (679-747). E-value: 2.6e-24. Score:
                     77.2. Matches aSDomain: nrpspksdomains_CE053_RS21120_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (757-1059). E-value:
                     4.6e-165. Score: 542.4. Matches aSDomain:
                     nrpspksdomains_CE053_RS21120_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1065-1219). E-value: 3.9e-53.
                     Score: 171.4. Matches aSDomain:
                     nrpspksdomains_CE053_RS21120_TIGR01720.1"
                     /codon_start=2
                     /gene="ppsC"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389714.1"
                     /locus_tag="CE053_RS21120"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="plipastatin non-ribosomal peptide synthetase
                     PpsC"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 7e-123, bitscore:
                     397.3, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.8e-16, bitscore:
                     47.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3e-79, bitscore:
                     253.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LGMFVNTLALRTRPEGGKPFVQYLQEVRETALEAFEHQDYPFEEL
                     VDKLELTRDMSRNPLFDVMFVLQNMDQESLELDELCLKPAANNGHQTSKFDLTLYAQEQ
                     PRGLLTFQMEFSTDLYKKKTIEKWLQYFINMLLSIIKDNKAALGTINILNEDETHYLIH
                     ELNRTKIDYPRNETISRLFEMQAEQTPNAVAIVSDTQVFTYENLNSWANQIASVLQIKG
                     VGPDSVVALLTGRTPELIAGMLGILKAGGAYLPIASDLPVERIAYMLSDSGAALLLQSE
                     KTEKQLLDIECEQIIIEGIQKQGETKNVDSSAGPHSLAYIIYTSGSTGKPKGVMIEQRS
                     VIRLVKNSNYITFTPEDRLLMTSSIGFDVGSFEIFGPLLNGAALHLSDQQTFLDSHQLK
                     RYIEHQGITTIWLTSSLFNHLTEQNEQTFSQLKHLIIGGEALSPSHVNRIRNVCPEVSI
                     WNGYGPTENTTFSTCLHIQKTYELSIPIGRPVGNSTAYILNQWGVLQPVGAVGELCVGG
                     DGVARGYLGRPDLTKEKFVPHPFAPGDRLYRTGDLARWLPDGTIEYVGRIDDQVKIRGY
                     RVELGEIESALRQIDGVKEAAVLARTAQTGSKELFGYISVKAGTNAEQVRSLLARSLPN
                     YMIPAYIIEMETLPLTSNGKLNRKALPEPNFTSKQTYAPPRNDLEDQLVLIWQEVLGIQ
                     RIGIEDSFFELGGDSIKALQVSARLGRYGLSLQVSDLFRHPKIKDLSPFIRKSERIIEQ
                     GPVQGDVPWTPVQQWFFSQDIEERHHFNQSVMLFHSGRLSENALRPALKKLAEHHDALR
                     MVYRNDGRRWIQINQGIHESQLYSLRTSDLSQSESGWETQIKQEVADLQQSINLQEGPL
                     LHAALFKTLTGDYLFLATHHLVVDGVSWRILLEDLSAGYQQAASGQTIQLPPKTDSYQE
                     YARRIQEYAQSSKLIREEAYWRSVEEQQAAELPYEIPHHVNIDFSKRDSLSFSLTEADT
                     AVLLQNVNYAYGTDTQDILLTAASLAICEWTGGSKLRIAMEGHGREHILPELDISRTVG
                     WFTSMYPALISFENHRDELGTAVKTVKDTLGRIPNKGVGYGMLKYLTHPENKSITFSKT
                     PEISFNYLGQFNDIERQDTFRPSSLGSGKDITHTWKREQIIEMSAMAADKKLHFNLSYP
                     SARFHKNTMEQLINRIEHFLLDIMKHCAGQQKAEKTLSDFSSQSLTAEDLDSISSLVEE
                     L"
     gene            complement(5804..>9485)
                     /gene="ppsC"
                     /locus_tag="CE053_RS21120"
                     /pseudo=""
     aSDomain        complement(5825..6286)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21120_TIGR01720.1"
                     /evalue="3.90E-53"
                     /label="CE053_RS21120_TIGR01720.1"
                     /locus_tag="CE053_RS21120"
                     /protein_end="1219"
                     /protein_start="1065"
                     /score="171.4"
                     /tool="antismash"
                     /translation="ELGTAVKTVKDTLGRIPNKGVGYGMLKYLTHPENKSITFSKTPEI
                     SFNYLGQFNDIERQDTFRPSSLGSGKDITHTWKREQIIEMSAMAADKKLHFNLSYPSAR
                     FHKNTMEQLINRIEHFLLDIMKHCAGQQKAEKTLSDFSSQSLTAEDLDSI"
     aSModule        complement(5825..8938)
                     /complete
                     /domains="nrpspksdomains_CE053_RS21120_AMP-binding.1"
                     /domains="nrpspksdomains_CE053_RS21120_PCP.1"
                     /domains="nrpspksdomains_CE053_RS21120_Epimerization.1"
                     /domains="nrpspksdomains_CE053_RS21120_TIGR01720.1"
                     /locus_tags="CE053_RS21120"
                     /monomer_pairings="Val -> D-Val"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(6131..6160)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0011"
                     /evalue="3.90E-03"
                     /label="NRPS-E7"
                     /locus_tag="CE053_RS21120"
                     /protein_end="1117"
                     /protein_start="1107"
                     /score="9.8"
                     /tool="antismash"
                     /translation="PEISFNYLGQ"
     CDS_motif       complement(6200..6262)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0010"
                     /evalue="1.20E-09"
                     /label="NRPS-E6"
                     /locus_tag="CE053_RS21120"
                     /protein_end="1094"
                     /protein_start="1073"
                     /score="30.4"
                     /tool="antismash"
                     /translation="VKDTLGRIPNKGVGYGMLKYL"
     aSDomain        complement(6305..7210)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21120_Epimerization.1"
                     /evalue="4.60E-165"
                     /label="CE053_RS21120_Epimerization.1"
                     /locus_tag="CE053_RS21120"
                     /protein_end="1059"
                     /protein_start="757"
                     /score="542.4"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="GDVPWTPVQQWFFSQDIEERHHFNQSVMLFHSGRLSENALRPALK
                     KLAEHHDALRMVYRNDGRRWIQINQGIHESQLYSLRTSDLSQSESGWETQIKQEVADLQ
                     QSINLQEGPLLHAALFKTLTGDYLFLATHHLVVDGVSWRILLEDLSAGYQQAASGQTIQ
                     LPPKTDSYQEYARRIQEYAQSSKLIREEAYWRSVEEQQAAELPYEIPHHVNIDFSKRDS
                     LSFSLTEADTAVLLQNVNYAYGTDTQDILLTAASLAICEWTGGSKLRIAMEGHGREHIL
                     PELDISRTVGWFTSMYPALIS"
     CDS_motif       complement(6311..6358)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0009"
                     /evalue="5.80E-09"
                     /label="NRPS-E5"
                     /locus_tag="CE053_RS21120"
                     /protein_end="1057"
                     /protein_start="1041"
                     /score="27.9"
                     /tool="antismash"
                     /translation="DISRTVGWFTSMYPAL"
     CDS_motif       complement(6764..6829)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0008"
                     /evalue="2.40E-12"
                     /label="NRPS-E2"
                     /locus_tag="CE053_RS21120"
                     /protein_end="906"
                     /protein_start="884"
                     /score="38.9"
                     /tool="antismash"
                     /translation="LFLATHHLVVDGVSWRILLEDL"
     CDS_motif       complement(7034..7117)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0007"
                     /evalue="5.60E-04"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE053_RS21120"
                     /protein_end="816"
                     /protein_start="788"
                     /score="12.4"
                     /tool="antismash"
                     /translation="SGRLSENALRPALKKLAEHHDALRMVYR"
     CDS_motif       complement(7172..7198)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0006"
                     /evalue="3.30E-03"
                     /label="NRPS-E1"
                     /locus_tag="CE053_RS21120"
                     /protein_end="770"
                     /protein_start="761"
                     /score="10.6"
                     /tool="antismash"
                     /translation="WTPVQQWFF"
     aSDomain        complement(7241..7444)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21120_PCP.1"
                     /evalue="2.60E-24"
                     /label="CE053_RS21120_PCP.1"
                     /locus_tag="CE053_RS21120"
                     /protein_end="747"
                     /protein_start="679"
                     /score="77.2"
                     /tool="antismash"
                     /translation="EDQLVLIWQEVLGIQRIGIEDSFFELGGDSIKALQVSARLGRYGL
                     SLQVSDLFRHPKIKDLSPFIRKS"
     CDS_motif       complement(7730..7795)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0005"
                     /evalue="1.10E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CE053_RS21120"
                     /protein_end="584"
                     /protein_start="562"
                     /score="39.7"
                     /tool="antismash"
                     /translation="YVGRIDDQVKIRGYRVELGEIE"
     aSDomain        complement(7760..8938)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE053_RS21120_AMP-binding.1"
                     /evalue="3.40E-115"
                     /label="CE053_RS21120_AMP-binding.1"
                     /locus_tag="CE053_RS21120"
                     /protein_end="574"
                     /protein_start="181"
                     /score="376.8"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEMQAEQTPNAVAIVSDTQVFTYENLNSWANQIASVLQIKGVGPD
                     SVVALLTGRTPELIAGMLGILKAGGAYLPIASDLPVERIAYMLSDSGAALLLQSEKTEK
                     QLLDIECEQIIIEGIQKQGETKNVDSSAGPHSLAYIIYTSGSTGKPKGVMIEQRSVIRL
                     VKNSNYITFTPEDRLLMTSSIGFDVGSFEIFGPLLNGAALHLSDQQTFLDSHQLKRYIE
                     HQGITTIWLTSSLFNHLTEQNEQTFSQLKHLIIGGEALSPSHVNRIRNVCPEVSIWNGY
                     GPTENTTFSTCLHIQKTYELSIPIGRPVGNSTAYILNQWGVLQPVGAVGELCVGGDGVA
                     RGYLGRPDLTKEKFVPHPFAPGDRLYRTGDLARWLPDGTIEYVGRIDDQVKIR"
     CDS_motif       complement(7877..7966)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0004"
                     /evalue="4.30E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="CE053_RS21120"
                     /protein_end="535"
                     /protein_start="505"
                     /score="57.3"
                     /tool="antismash"
                     /translation="PVGAVGELCVGGDGVARGYLGRPDLTKEKF"
     CDS_motif       complement(8471..8527)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0003"
                     /evalue="1.00E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="CE053_RS21120"
                     /protein_end="337"
                     /protein_start="318"
                     /score="33.3"
                     /tool="antismash"
                     /translation="AYIIYTSGSTGKPKGVMIE"
     CDS_motif       complement(8714..8752)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0002"
                     /evalue="1.60E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="CE053_RS21120"
                     /protein_end="256"
                     /protein_start="243"
                     /score="15.0"
                     /tool="antismash"
                     /translation="LGILKAGGAYLPI"
     CDS_motif       complement(9281..9400)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE053_RS21120_0001"
                     /evalue="8.80E-23"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CE053_RS21120"
                     /protein_end="67"
                     /protein_start="27"
                     /score="72.8"
                     /tool="antismash"
                     /translation="RETALEAFEHQDYPFEELVDKLELTRDMSRNPLFDVMFVL"
ORIGIN
        1 ggtaacggcc gcctctctta caccttctat acttcttagt gccgcttcaa tttctcccgg
       61 ctcaatccgg tagccgcgga ttttcacctg atcatccgtg cgcccgagga attcaacatt
      121 cccgtcaggg agccagcgcg ccacatctcc tgttttgtac atacgttcac ccgggtaaaa
      181 cgggtcttct agaaaacgct cctccgtcaa tgccggccga ttcaaataac ctctggcaac
      241 acccgctccg gcaatgtata gttcgccgat acatccagca ggcagcatcc gctgattctg
      301 attcaggata tataatctga catgatgaac aggttttcca attggaatcc gttcaagttt
      361 ttcatgcagc ggacaatcaa agaatgcagc ttctacggtt gcttccgttg gcccatagga
      421 attggttatt gaaacatttg gaagcaactc gtgaaatcgg gagacaagat gggtccccag
      481 ctgttctcct ccagaaaaca ctcgcttgag tctgtttgtc ttaatcggta cagagcgata
      541 ttttatatgt tctaaaaatg catggagcat tgaaggcaca aaatgcatag ctgtgatctt
      601 ttgttcttct atggccttca cgatcacttc aggttctttt tcgcctccct gaggaagcag
      661 ataaacagaa gctccggcat aaggccacca aaacagctcc catattgaag catcgaatga
      721 atacgatgtt ttgtgcataa tcatgtctga atgctttagc tgatagcggg actgtaggga
      781 attcaaaaaa ttcaccgctg atcgatgttc aatttcaaca cccttaggca tcccggtaga
      841 gccggatgta tagatgacat aggctaaaga ttgcgggaga acatccacat tcggattgga
      901 caaacgatgt gccagtccgc tatttttctg attgagatag acaatctctc ctgtaaattc
      961 cggaacatga aggccggcag actgaagcag gagaatgttt gtaccgctgt cctccagcag
     1021 aaagctgatt cgttcttccg gataatccgg gtcgatgggc agataagcgc cgccagcttt
     1081 aagaatgcca taaatgccaa gcagcatttc aggtgaacgc tttcctataa tggcaacagt
     1141 cgtttcgggt tttacccccc gtccgatcag ctcccaggcg atacggttag cctgttcgtt
     1201 caattcttta taggtcatca ctccatcttc gaagataacg gccgcctgat caggcgtttt
     1261 tgctgcctgc tgttcgagtg catagtggag cgtttgattc attgaatagc ccgtctcctc
     1321 aaacagatgg catgattcaa tgaaatctcg ctcttcttct tttgttaaga ttggaatatt
     1381 gtccaattct gtttcagggc tggcagctgt atgttccaca agcgtggtga aaaggctggc
     1441 ccacctttgg atggtttctt tctcaaacaa agctgtactg tattcaaact gacagaaaag
     1501 ctcggaatca tgttctgtaa cgtataggga aatatcaaat tttgatattg ggtgcaccaa
     1561 gtcgttccac tgaagctgta catcattcat ttttagcttt tgctgttcca tattctgaag
     1621 gactagcgtt gtgtcaaaca aaggattccg gctcatttcc cgctgcacac cgagtttgtc
     1681 cacaagctct tcaaacgggt aatcctgatg ctcatacgct tctagtgccg tctgtctgac
     1741 ttctttcaga aattcctcaa acgtcttatt acccgccggg cgtgtgcgga gagccagtgt
     1801 attgacgaac atcccaagaa cagcttcaag gtcggcatgg ggccgtccgg cgataggtga
     1861 accaatcacg atatcatcct gaccgcttaa ccgagacaaa aaggctgaat acaaagcgag
     1921 aagcgtcata taaagcgtgc ttctgttttt atgggcgagc cgctttatgc gggccgtgat
     1981 ctctttgtcc aattgaaagg ttacttttcc gcctatgaat tgacgttctg ccggacgtcg
     2041 tttatctgtt ggaagctcca gtataggaag atccccgcta aactgctgct gccagtaagt
     2101 ctcctgcttt tggtacgagt ctcccttttt aaatttctgc tgccatacgg cgtaatcttt
     2161 atattggatc cgcaatggct taagctgccg gtttgcataa agatcggtaa attctcggat
     2221 taggagctga acggatacgc catctgaaat gatatggtgc atatccacca gcaatacatg
     2281 cttctcgttt gacacttcta taaggcctgc tctaaataat ggcgcctggc tgagatcaaa
     2341 gggacggaca aatccctcga tagtggtctc tttacttaac gtaaacggca cttcggtgtg
     2401 gatacgctga accggctcgt catcagctcc ggatacaaag gaagttcgaa gcggctcatg
     2461 ccggctgatc agttctttac atacagcaga cagtttggcg acatccagtg caccttccag
     2521 ttctaagacc gcaggcatgt tataggcgac accctcatca agctgttgaa gcacatagat
     2581 tcgtttttgg gccgaagata caggatatgt atcccttttt tctgctggtt caattgctgc
     2641 atacggattt tgttctgcct ctcgaatgat tgaagctaat ccttcaacag tcggatgggc
     2701 aaagatgtcc tttagcggaa cttgcacatg aaactcctta gcgatccgtg acataagcac
     2761 tgttgctttt aacgagtggc cgccatggtc aaaaaagttg tcttggatgc ctatatggcc
     2821 attattcagt acatcttcaa atagttgtga aagctttgcc tctaacagat ttcgaggggc
     2881 ggtatatggg tttccgctga ttgaagcatc tggctccgga agtgctgcgc gatctacttt
     2941 tccgtttggg gtaagaggta tgctgtcaac ccatatccat ttcgccggaa tcatgtattc
     3001 tgggagctca ctagccatat aactgcgtaa atctattgcg ttcagctttt gtacactgca
     3061 atagtaagcg cataattcat gttcaccgtt ttgatcttgc cagatgagaa tggcagcctc
     3121 actaatgtcc ggatgattca ttagcgtgac ttcgatttct tgcggctcaa ttcgataacc
     3181 tctgattttc acttgattgt ctgcccgtcc gatatattcg atgttgccgt cttcaagccg
     3241 tctggccaaa tcacctgtcc ggtacatgat cacatcaggc ttgaatggat cggatgaaaa
     3301 tttgtcgctt gtcagtttat cgtgattgac atatcctctg gcaacacttg cgccggaaat
     3361 atagagttcg cctggtacgc cgcatggctg aagctgtttt tgatgattca aaatatatag
     3421 atcagtacag cctatcggtt taccgattgg cggaagctca ggaatagggt ctccaggatg
     3481 aatggtatac gtagatacca catgagtttc tgaaggtccg taatgattgt gcaggtgtat
     3541 gccgcgttta cgcaacacat cttggaaaag gtctgaaatc attaattgtt ccccggctgc
     3601 gatcaggtgt ttgacgccat caggaaacga gttcgcaagt tctcgctcgc tgaaaatcat
     3661 tttgataaaa gcagttggga gaaacacaat attcgtctga tgcttgttta taaatgcaaa
     3721 cagctgaggc acatctcttt ttatggcttc tggcacgatg tgcagtgtgc cgcctttcag
     3781 aagcgcagaa aaaatttctt gatagcagac gtcgaaggaa ggcgtcgcaa attgcagaac
     3841 atctgcttca aagtcaatgc cggagtgagt atattcgaat ttcagcaagt tagccatgtt
     3901 tcgatgctca aattgaacac cctttggttt acctgttgtg cctgatgtat aaatcatata
     3961 cagcagatcg tcagcattgt tgactgactt tatgttactg ccgtctactt tgctttcgat
     4021 atcttctgga tgagtgataa tgtaattgtc ttctaacgat gaggaaagat gcgcttgtga
     4081 aacaacatgc gaacagccgc aatcatgcag aatataacgg attctgtcag ccgggtaatg
     4141 cggatcaacc ggaacaatgg cgccgcctgc ctttaagata ccaagaacac ctgtgataaa
     4201 cgccgctgag cgttccatca taatcgcaac gggttcctgt ctcacaacgc ctttcattcg
     4261 taaatgccgt gccaatctgt tagccgcttt attcagttct ctgtatgtta atgagcaggc
     4321 ttgatcaatt acagcatgag catctggtgt cttttccgct tgcttttcaa acatggcatg
     4381 gatggtttcg taaactggtg tttgatctgt gatttctcca atcagctgcc gcttttcagt
     4441 atctgtcgtg atatcgattt cttcaggtgg aagattcgga ttatttaaga tttgtgatac
     4501 agcattcaga agatgctctt tgacagagag cacaaaagac cgatcgtgct gctccgcatc
     4561 atagttcatt ttgatatgaa tttcttcccc tggcgccacc ataagataaa agctgtagtt
     4621 ggtttcatct gcgacagtga aattatgaat ctgaaaaaga tcagaagcat gttcctgttt
     4681 gttcagcatt tgaatctctt gctgcaccgg atagttttca aatacgagaa tatgatcaat
     4741 cagcccttgt ttaacagctg agcgggattg aatctcatat aaagggtggt agctgtacgc
     4801 ttcagcggca agacggtctt tttgcatatc ttttataagt tgaagaaatg gcgtttttgc
     4861 cccctgcacc cgaaccggca tcgtattaat gaaaagacct accatatgtt caatgccttc
     4921 gatttctgcg ggtcttcctg aaatgacaga accgaatacg gcgtcatcgt tattattata
     4981 tttttgtagc agaatgcccc aaatggtatg aaaaatcgta ctgagtgtca cttcttctct
     5041 tgcagcaagc tctgaaagtc tgctgctttc ttccttagag aatgaaaatg taacgtgttc
     5101 ctgcctgctt cttccctttg tttttttctt ctgcttcggg agcacggttt gctgttcgtg
     5161 gccggaaaga taatggtccc aataagaaac cgcctgctct ttgtcttgct ccatcagcca
     5221 tttgatatac tttccgtatg gctggaccgg ctctaatgtt ataggggatg catttacata
     5281 agatgcgtaa atatgcagaa actcctttag cacaatgctg aggcaccaac cgtccatcat
     5341 gatatgatga aaggtccaaa tgcaagagta ctggctctct cctgtttgaa taagagcgat
     5401 gcgcatgagc acatcctttt gcaaatgaaa cccccggtcc cggtcctttt ctttccattg
     5461 ctcgataaac tgtgattgct ccgcctcgtt taaatgagta atgtcttctt ccaggacgat
     5521 gatatttcgt tctctcagaa caacctgctg gggggaggaa acattttggc tgataaaaat
     5581 cgttctgaaa atatcatgtc tttctacaag agcgttgatg ctgttttgaa aaaactgcgg
     5641 gttgactttt ccttctattg taaaagaagc ttgctcaaca tacgcctgtg aatctttttg
     5701 caggagggaa tggaaaagca tcccttcctg catgtaagac agcggataaa tatcctgaat
     5761 tgaattcgct tttgtcatgt tcatgtctcc ctgtcacgat tttctacaat tcttccacca
     5821 agctggatat gctgtccaag tcctctgccg ttaatgattg actgctgaaa tcgctcagtg
     5881 tcttttctgc tttctgttga ccggcgcagt gtttcataat gtccagcaaa aagtgttcaa
     5941 tcctgttaat aagctgctcc attgtgtttt tgtgaaagcg ggctgacgga taactaagat
     6001 tgaaatggag ctttttgtct gctgccattg cgctcatctc gatgatttgc tcacgcttcc
     6061 atgtatgagt gatatccttt ccgctaccaa ggcttgatgg gcggaaggtg tcctgtcttt
     6121 cgatgtcgtt gaactggccc aaatagttaa agctgatttc aggtgttttt gaaaacgtaa
     6181 tgcttttatt ttcagggtgt gtaaggtatt tcagcatacc gtaacctacc cctttgtttg
     6241 gtatccggcc cagtgtatct ttaacggttt ttactgcggt tccgagctca tctctatgat
     6301 tttcaaagct tatgagggcc gggtacatgg atgtaaacca gccgaccgtc ctgctgatat
     6361 ctaactctgg cagaatgtgt tctctcccgt gtccttccat agcgatacgg agcttgcttc
     6421 cgcctgtcca ttcacaaatc gccaaggatg ctgcggttaa cagaatatct tgtgtatctg
     6481 tgccataggc gtaattgacg ttttgtagca agacggcggt gtctgcttca gtcagtgaaa
     6541 agcttaatga gtcccttttg ctgaagtcga tgttcacatg atgcggtatt tcatatggca
     6601 gttctgcggc ttgctgttct tctacacttc gccaatacgc ctcttcgcga atcagcttac
     6661 tgctttgggc atattcctgt atgcgtcttg cgtattcctg gtaggaatcc gttttaggcg
     6721 gaagctggat cgtctggcct gaagcagctt gttgatatcc ggctgacaaa tcctccagca
     6781 aaatccgcca tgagacacca tcaacgacaa ggtgatgggt cgcaaggaac aagtaatctc
     6841 cggtcagtgt tttaaacaaa gcggcgtgca acaacggtcc ttcctgcaaa ttgatgcttt
     6901 gctgaagatc tgcaacttct tgtttgattt gcgtctccca gccgctttct gactgtgaaa
     6961 gatcagaggt tcttaagctg tatagctgtg actcatgaat gccttggttg atttgaatcc
     7021 atcgcctgcc atcgttgcga taaaccatcc gaagcgcatc atgatgttcc gccagttttt
     7081 tcaaggctgg tctaagggcg ttttcagaca aacgtccaga atggaatagc atgacagact
     7141 gattgaaatg gtgtctttct tcaatatcct gtgaaaaaaa ccattgctga acaggcgtcc
     7201 aaggaacgtc accctgaacg ggtccttgct ctatgattct ctctgactta cggataaatg
     7261 gtgataagtc ctttatcttt ggatgacgga ataaatcact tacctgcaag cttagtccat
     7321 agcgcccaag gcgtgcagaa acttgaaggg ctttgattga atctccaccc aattcaaaaa
     7381 acgaatcctc tattccgatc ctctggatgc cgagaacttc ctgccagatc aacacaagtt
     7441 ggtcttcaag gtcatttcga ggtggagcgt acgtttgttt agatgtgaaa tttggttcag
     7501 gaagtgcttt tcggttcaat ttgccgtttg atgtgagcgg gagagtctcc atttcaataa
     7561 tgtacgccgg gatcatatag tttggcagcg aacgcgcgag aagtgaacgt acttgttcag
     7621 cattcgtgcc tgcttttacg ctgatatagc caaacagctc tttgctcccg gtctgggctg
     7681 tacgtgctaa cactgcggct tctttgaccc cgtctatttg acgaagtgct gattcgattt
     7741 cacccagctc cacccgatag cctctgattt ttacttgatc atcaatacgc ccaacgtatt
     7801 caattgtgcc atcaggaagc cagcgcgcca gatcacctgt gcgatacagt cgatcgccag
     7861 gtgcaaatgg atgtgggaca aatttctctt tcgttagatc aggacggccc aaatatcctc
     7921 tggcgacgcc atctccgccg acacaaagtt caccgacagc tccgaccggc tgcagcactc
     7981 cccattgatt taagatataa gcggtggaat taccaaccgg ccgcccgatc ggaatggaaa
     8041 gttcatatgt tttttgaatg tgaaggcatg tcgaaaaagt tgtattttcg gtagggccat
     8101 atccattcca tatagatact tcagggcaga catttctgat tcgattgacg tggctcggcg
     8161 acaaagcttc tccgccaata atcaaatgtt tcagctgtga aaatgtttgt tcattttgct
     8221 ctgtcaaatg attgaataga gaagatgtca gccaaatcgt tgtgattcct tgatgttcta
     8281 tgtacctctt aagctgatgg gaatcgagaa atgtttgttg atcagacaga tgcaaagcgg
     8341 cgccatttag cagcggtccg aaaatttcaa atgacccaac gtcgaaaccg attgatgatg
     8401 tcataagaag gcggtcttca ggtgtaaatg taatataatt gctgtttttt acaagacgaa
     8461 ttacacttcg ctgctcaatc atgacaccct ttggtttacc ggttgaacca gaggtataaa
     8521 tgatatatgc aagtgaatgt ggccctgctg aagaatcaac gttcttcgtt tctccttgct
     8581 tttgaatacc ttcgattatg atttgttcac attcaatgtc taatagctgt ttctctgttt
     8641 tttcagattg aaggagtaat gccgccccgc tgtctgacag catataagct attcgttcaa
     8701 caggcagatc agaagcaata ggcagatagg ctcccccggc ttttagaatg cccagcattc
     8761 ctgcaatgag ttctggtgtt ctgcctgtca acaaggctac aacactatca ggccctacgc
     8821 ccttaatctg tagaactgag gcaatttgat ttgcccagct gttcaggttc tcgtaagtaa
     8881 atacttgggt gtcgctgaca attgctacag cattaggcgt ttgttcagcc tgcatttcaa
     8941 agagtctgct gatggtttca tttcttggat aatctatttt tgtacgattg agctcgtgga
     9001 taaggtaatg ggtctcatcc tcattcagga tatttattgt tccgagagcg gctttgttgt
     9061 ctttgatgat ggagagtaac atattgatga agtattgaag ccacttttct atcgtttttt
     9121 tcttatataa gtcagtacta aactccattt ggaacgtcag caaaccgcgg ggctgttcct
     9181 gggcatatag ggttaaatca aatttggacg tttgatgccc attgtttgct gcaggtttga
     9241 gacaaagttc atccagttcc aacgattcct gatccatgtt ttgaagaaca aacattacgt
     9301 caaacagcgg atttctgctc atatcccgtg tcaattcaag cttatcaaca agctcttcga
     9361 acgggtaatc ttggtgttca aaggcttcca atgctgtctc acgcacttcc tgcaaatact
     9421 gtacgaatgg cttcccgccc tccgggcgcg tccgaagcgc cagtgtgttc acaaacatgc
     9481 cgagt
//
