LOCUS       NZ_CP048397            10522 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. S4.7 chromosome, complete genome.
ACCESSION   NZ_CP048397
VERSION     NZ_CP048397.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. S4.7
  ORGANISM  Streptomyces sp. S4.7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7920066)
  AUTHORS   Bekiesch,P., Zehl,M., Domingo-Contreras,E., Martin,J.,
            Perez-Victoria,I., Reyes,F., Kaplan,A., Ruckert,C., Busche,T.,
            Kalinowski,J. and Zotchev,S.
  TITLE     Viennamycins: new lipopeptides produced by Streptomyces sp. S4.7
            isolated from the rhizosphere of Edelweiss (Leontopodium nivale
            subsp. alpinum)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7920066)
  AUTHORS   Bekiesch,P., Zehl,M., Domingo-Contreras,E., Martin,J.,
            Perez-Victoria,I., Reyes,F., Kaplan,A., Ruckert,C., Busche,T.,
            Kalinowski,J. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2020) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.8x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010384365.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 10:27:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,027
            CDSs (total)                      :: 6,939
            Genes (coding)                    :: 6,650
            CDSs (with protein)               :: 6,650
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 103 of 289
            Pseudo Genes (incomplete)         :: 223 of 289
            Pseudo Genes (internal stop)      :: 23 of 289
            Pseudo Genes (multiple problems)  :: 52 of 289
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7683489
            Orig. end                         :: 7694011
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048397.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zotchev Lab, University of
            Vienna, Austria.
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10522
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="31"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10522
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10522
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5522](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5522
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(893..1879)
                     /locus_tag="SSPS47_RS33480"
                     /old_locus_tag="SSPS47_34025"
     CDS             complement(893..1879)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016827480.1"
                     /locus_tag="SSPS47_RS33480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_34025"
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="WP_164254164.1"
                     /transl_table=11
                     /translation="MRRTPTQVALVAATALTLVACGGGGDDKDSQPPSAPTGLSVEAGS
                     ALSAHVMWEAAKDNVAVSGYVVYRGDRKVKDVPPEKLMIDVTDLKPSTAYSFSVRAKDA
                     AGNLSPHSEKKPVTTPAVVAEDKEAPAKPTKLRGKHDGSHGAELTWSAPGKGQGITSYD
                     ILQSGSKIHSVGGDATSTKVTGLRPGTDYSFTLQARDAADNTSPASAPVEISTPKGPGD
                     DPDTAPLSFKVERHKSAEDGAYYLDLSWLPPKVDGEVPSYEIHLNGKLTTTLVWGNKAP
                     VGKTTESVFAGKKPGVSYKVKIRAKLPDGEWGSFSPELTMVTGEAKS"
     misc_feature    complement(1909..1924)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.87"
                     /tool="antismash"
     gene            2077..2871
                     /locus_tag="SSPS47_RS33485"
                     /old_locus_tag="SSPS47_34030"
     CDS             2077..2871
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (8-124). E-value: 3.2e-10. Score:
                     32.2. Matches aSDomain:
                     nrpspksdomains_SSPS47_RS33485_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062680.1"
                     /locus_tag="SSPS47_RS33485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_34030"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_164254165.1"
                     /transl_table=11
                     /translation="MQIDLAGKTALVTGSTQGIGAAIVTGLARAGARVAVNGRSEESVS
                     AALDSRRAECPGAEFLAAPGDVSTESGAAQVLEAVPRVDILVNNLGIFGRRPALEISDD
                     EWRRYFEVNVLAAVRLTRACLGGMTERGWGRIQYIASDSAIVVPAEMIHYGVSKTALLG
                     VSRGFAKAAAGSGVTVNSVLAGPTHTGGVEDFVYELVDKSLPWDEAQREFMREHRPQSL
                     LQRLIEPEEIANMVVYLSSPLASATTGAAVRVDGGYIDSIVP"
     aSDomain        2101..2448
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SSPS47_RS33485_PKS_KR.1"
                     /evalue="3.20E-10"
                     /label="SSPS47_RS33485_PKS_KR.1"
                     /locus_tag="SSPS47_RS33485"
                     /protein_end="124"
                     /protein_start="8"
                     /score="32.2"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TALVTGSTQGIGAAIVTGLARAGARVAVNGRSEESVSAALDSRRA
                     ECPGAEFLAAPGDVSTESGAAQVLEAVPRVDILVNNLGIFGRRPALEISDDEWRRYFEV
                     NVLAAVRLTRAC"
     CDS_motif       2107..2196
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SSPS47_RS33485_0001"
                     /evalue="3.50E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="SSPS47_RS33485"
                     /protein_end="40"
                     /protein_start="10"
                     /score="13.3"
                     /tool="antismash"
                     /translation="LVTGSTQGIGAAIVTGLARAGARVAVNGRS"
     gene            2900..3412
                     /locus_tag="SSPS47_RS33490"
                     /old_locus_tag="SSPS47_34035"
     CDS             2900..3412
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006132250.1"
                     /locus_tag="SSPS47_RS33490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_34035"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_239065172.1"
                     /transl_table=11
                     /translation="MQTNTLVVRRAVPADAPDISGLWLRSFAAALPSVTRPHSEQRIHA
                     WIRDIVLPTQETWVATVEETVIGMMVIEGGDIDQLYLDPDHRGCGIGDRLLELAKRRRP
                     GGLSLWTFQVNGPARRFYERHGFVPVEKTDGRGNEENEPDVHYSWRPEPGGGGIPGPAK
                     EPDPAAH"
     misc_feature    complement(3843..3858)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.37"
                     /tool="antismash"
     gene            3942..4838
                     /locus_tag="SSPS47_RS33495"
                     /old_locus_tag="SSPS47_34040"
     CDS             3942..4838
                     /GO_function="GO:0004568 - chitinase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006032 - chitin catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452937.1"
                     /locus_tag="SSPS47_RS33495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_34040"
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="WP_164254167.1"
                     /transl_table=11
                     /translation="MRQRILALFTALVSAMALAVFLPASTSAAATSAAACTTGWNASTV
                     YWGGDTASYDGRNWTAKWWTQNERPGTVDVWADNGACGGGPTDPPPAGGFVVSEAQFNQ
                     MFPSRNSFYTYNGLKAALSAYPAFATTGSDTIKRQEAAAFLANVSHETGGLVHIVEQNT
                     ANYPHYCDSSQWYGCPAGQAAYYGRGPIQLSWNFNYKAAGDALGIDLLGNPWLVQNDAA
                     VAWKTGLWYWNTQNGPGTMTPHNAMVNSRGFGETIRSINGSLECNGGNPAQVQSRIAKY
                     QSFVQILGTTPGGNLGC"
     gene            5001..5522
                     /locus_tag="SSPS47_RS33500"
                     /old_locus_tag="SSPS47_34045"
     CDS             5001..5522
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613014.1"
                     /locus_tag="SSPS47_RS33500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_34045"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_239065173.1"
                     /sec_met_domain="melC (E-value: 2.1e-39, bitscore: 133.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGGTQSQSRPVQARPAEPAAPAPPCPRHDPPVSRAPAPPPPPSL
                     PPSRSRRLFLRAAFALGVIGGTATALLPVLRADPRTAGAPPAEAAGPPPVPAKGAELFD
                     EMYGGRHIQGTDAGVLIDGRPLHVMRRADGSHLSVVNHYESFPTALATARAAVDDLGGA
                     QLSLAGPAFH"
     gene            5545..6411
                     /locus_tag="SSPS47_RS33505"
                     /old_locus_tag="SSPS47_34050"
     CDS             5545..6411
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267933.1"
                     /locus_tag="SSPS47_RS33505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_34050"
                     /product="tyrosinase family protein"
                     /protein_id="WP_164254168.1"
                     /transl_table=11
                     /translation="MHIRKNQRNLTSAEKKRFVATVLELKRQGRYDEFVRQHVQYYRAD
                     GDEGLRAAHMAPSFLPWHRKYLLEFERALQRIDPKVTVPYWDWTTDNTPAASLWSEDFL
                     GGDGRSSDGQVTTGAFAYGAGNWTIDDGVLERKFLVRAFGRPTSPIALPTKAELAWAMD
                     ESVYDAAPWDSTAPRGFRNRLEGWTAASDKTRWRNHNRVHRWVGGQMLGATAPHDPVFW
                     LHHAFVDLLWDRWQKKHPKATYLPQRKLGGGDPQRGQAISVDEPMPPWRTKPSEMLSHR
                     SLYRYEA"
     gene            complement(6568..6840)
                     /locus_tag="SSPS47_RS33510"
                     /old_locus_tag="SSPS47_34055"
     CDS             complement(6568..6840)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449724.1"
                     /locus_tag="SSPS47_RS33510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_34055"
                     /product="chaplin"
                     /protein_id="WP_147877932.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVLAGTGAVLAGGAGMAAADAGAEAVAAGSPGVLSGN
                     VVQVPVHVPVNLCGNTIDVIGLLNPAFGNQCVNADAKHHSGGYGR"
     misc_feature    complement(6825..6837)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.72"
                     /tool="antismash"
     gene            7061..8557
                     /locus_tag="SSPS47_RS33515"
                     /old_locus_tag="SSPS47_34060"
     CDS             7061..8557
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546067.1"
                     /locus_tag="SSPS47_RS33515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_34060"
                     /product="MFS transporter"
                     /protein_id="WP_164254169.1"
                     /transl_table=11
                     /translation="MSAAAIPEPGPAPALEPVEGRRRRFLVAVLAFCGVVVAVMQTLVV
                     PLLPHVPALTGSTPAAASWLVTITLLTGAVFTPVLGRVGDMYGKRRVLIASLGVLVAGS
                     VMCAVSSHIGVLIAGRALQGAALAVVPLGISIMRDELPPARLLSSVALMSSTLGIGAAV
                     GLPVAALVVQNFDWHTMFWASAVLGLIDIVLVLCCVPESPLRSRGRFDVIGTLGLSATL
                     VSLLLAVTQGADWGWTSARTVGLLGAALTIGLLWGAYELRTTSPVVDLRVSARPAVLLT
                     NIAALLTGFAFYANSLVTAQMVQEPTSTGYGIGASIVVGGLCLLPGGLAMVALSPVSAR
                     ISAKYGPKTALALAAAVMAVGYVVRFFTSHSLWPIIGGATVVAAGTAIAYSALPALVMR
                     AVPAGETGAANGLNTLMRSVGQAFCSAVVAAVLANITFQAGGRTAPTLHAYLLVFLIAA
                     GAALLALAVTLCLPGRAVAAEAGTVGKEQTASRTFAEEGA"
     gene            8554..9273
                     /locus_tag="SSPS47_RS33525"
                     /old_locus_tag="SSPS47_34065"
     CDS             8554..9273
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555865.1"
                     /locus_tag="SSPS47_RS33525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_34065"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_239065174.1"
                     /transl_table=11
                     /translation="MTTGHAADGTTVPGTDAGVGPDPDPDPDPDPDPGPDPDPDPDPDP
                     DPDPGPGRLAILRAARRAFTQRPYAEVTMRGIAADAGVSASLIVKRFGTKERLFNTVAD
                     FGPAADKLFAAPPAVLGRHLVITMVRLRRENHSDPLLRVVFSLGNMDERTLLRERFREQ
                     VTARLAGLIGGERSELRAELITGQLLGLGATLSLHRPGAGEEATEELLADLYAPALQRL
                     ITGEPDHPDQGPGRAGR"
     misc_feature    complement(9501..9512)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.78"
                     /tool="antismash"
ORIGIN
        1 ggtgcggacg gtgggcacgg ccgagggggc ggagctgatg gacatcagcc ccggccgctt
       61 cacccggctg gcgcgtgcgg ggtatctggt gccggtgcgt ttctatctca atcgctatcg
      121 agcggtcgtg tggctctatc tcgccgagga gctacgggcg ttcgcgagcg gtgagccggg
      181 cttgccggcg gggcggttgc cgcgatccgg gacacgtgcg gggccacgga agccgaccgg
      241 ccgtgcccgc tgcggctcgg acatgggcgt ggtgcccgac gccaaggcag gggaagcggc
      301 cggggtgatg gtgccggagg aggactgccg tccgcgcaac tggcgaggtc gccgtatcgg
      361 cctgctgctc cacgagagcg acgaccggtg ggagcgtgcg gcgatcgtcg gttcggtgct
      421 cgatcccgtc cagctggccg agctggttcc ggatccgtac gagcgcgcgt gtctgaaccg
      481 gttgcggccg gatctcgcgc cggggcgtcc cgggtcggag gcggggcgcg aggtcatggg
      541 gcggctgctg accgccgacg aaccggacga gatcagctgg caccgctcca gcctgaccgc
      601 gctgctggcc gacgcccgac gggaccggca ggcgccgcgc ccgagcggca cggctcctac
      661 tctgacgcgc gcacaggaaa ccccgtccgc cgaggattcg gcgcggacca ctgattcggt
      721 acgggccacc ggttcggtcg ggacgtccga accggtcccg tacggcggcg gacgcggacg
      781 tccgttgctg acccggctga ggggcaggag agtgcggggc cgagaggcgg cgttgcctct
      841 cggcggcacc gccgaccgtt gagttcgagc cctgcccggc ccaccgggcc ggtcaggact
      901 tggcttcgcc ggtgaccatc gtgagttcgg gggagaagct gccccactcc ccgtccggca
      961 gcttcgcgcg gatcttcacc ttgtagctga caccgggctt cttgcccgcg aagacgctct
     1021 cggtggtctt gcccacagga gccttgttgc cccagaccag cgtggtggtc agcttcccgt
     1081 tgagatggat ctcgtacgag ggcacctcgc cgtcgacctt cggaggcagc caggacagat
     1141 cgaggtagta ggcaccgtcc tcggcgctct tgtgccgttc gaccttgaag ctgagcggtg
     1201 ccgtgtcggg gtcgtcgccg ggtcccttcg gggtgctgat ctccacgggg gcgctggccg
     1261 gtgaggtgtt gtcggcggcg tcccgcgcct ggagggtgaa cgagtagtcg gtgccggggc
     1321 gcagccccgt gaccttggtc gaggtcgcgt cgccgcccac gctgtggatc ttcgagccgc
     1381 tctggaggat gtcgtacgag gtgatgccct ggcccttgcc cggtgcgctc caggtcagtt
     1441 cggcgccgtg cgaaccgtcg tgcttgccgc gcagtttcgt cggcttcgcc ggggcttcct
     1501 tgtcctcggc gaccaccgcg ggtgtggtga ccggcttctt ctcgctgtgc ggcgagaggt
     1561 tgcctgccgc gtccttggca cgcaccgaga aggagtaggc ggtggacggc ttcagatcgg
     1621 tgacgtcgat catcagtttc tcggggggaa cgtccttgac cttcctgtcg cctcggtaga
     1681 ccacgtagcc gctgaccgcg acattgtcct tggccgcttc ccacatgacg tgtgcggaca
     1741 gtgcgctgcc ggcctcaacg ctcaggccgg tcggtgcgga tggcggctgt gagtccttgt
     1801 cgtcaccgcc gccgccgcag gcgacgaggg tgagagcggt agcggcaacg agcgcgacct
     1861 gggtgggggt ccgtcgcacg atcgtctcct ccgtccaagg cgaggggcaa tggtctagac
     1921 atatatgaca cagtgtcggg ttcacttcaa gagctggtgc cagttggaat gcgaaatcga
     1981 gggaagccga cactccgccc ggccagcgga ccgggccgta cgcggacccc cacgcacaat
     2041 gacctcgatc caccgacaat cgggaaggaa ctgaacgtgc agatcgatct cgcaggcaag
     2101 accgccctgg tcaccggctc cacacagggc atcggggcgg ccatcgtgac cggactcgcc
     2161 cgcgcgggag cccgcgtcgc ggtcaacgga cgctccgagg agtccgtgtc cgccgccctg
     2221 gacagtcggc gtgccgagtg tccgggcgcg gagttcctgg ccgcgccggg tgacgtctcc
     2281 accgagagcg gagccgcgca ggttctcgaa gcggtgccac gggtggacat cctcgtcaac
     2341 aacctgggca tcttcgggcg gcggcccgcg ctggagatca gcgacgacga gtggcggcgc
     2401 tacttcgagg tcaatgtcct cgccgcggtc cggctcactc gcgcttgtct gggcgggatg
     2461 accgaacgtg gctggggccg catccagtac atcgccagtg actccgcgat cgtcgtcccc
     2521 gccgagatga tccactacgg agtctccaag accgcgctgc tgggggtgtc gcgcggcttc
     2581 gcgaaagcgg cggcgggcag cggcgtcacc gtgaactccg tgctcgcggg acccacgcac
     2641 accggcggcg tcgaggactt cgtctatgag cttgtcgaca agtcccttcc ctgggacgag
     2701 gcccagcgcg agttcatgcg tgagcaccgg ccgcagtcgc tcctccagcg gctcatcgaa
     2761 cccgaggaga tcgcgaacat ggtcgtctat ctgagctcgc ctctcgcgtc cgcgaccacc
     2821 ggggccgcgg tgcgtgtcga cggcggatac atcgactcga tcgttccgtg atttctcgtt
     2881 gatgacagga tgggtcgctg tgcagaccaa cacacttgtc gtccgtcgcg cggtgcctgc
     2941 cgacgcgccc gacatctccg gactctggct gagatcgttc gcggccgccc tcccgagtgt
     3001 cactcgcccc cacagcgaac agcggataca cgcctggatc cgggacatcg tcctgcccac
     3061 ccaggagacc tgggtggcga ccgtcgagga gacggtgatc ggcatgatgg tgatcgaggg
     3121 cggggacatc gaccagctgt acctcgatcc ggaccatcgg ggctgcggta tcggcgatcg
     3181 cctgctcgaa ctggcgaagc ggcgtcggcc cggcggactg tcgctgtgga cgttccaggt
     3241 gaacgggccc gcacggcgtt tctacgagcg ccacggcttc gtcccggtgg agaagaccga
     3301 cggccggggc aacgaggaga acgaaccgga cgtgcactac agctggcgcc ccgaaccggg
     3361 cggtggcggc atccccggtc ccgccaagga gcctgatccg gcggcccact gaacaggaac
     3421 cgtgtcacgc gcgttcgacg ggccggggcg cgatcgccgt cgtatccgtg aacacgtcgc
     3481 cgtccggcgc cttcccggcg gcgacgaaga ccggtggaac aggagcgaac agcacctgcc
     3541 cggccgcagt ggttgtatcg cgcgtcgcct cgcgccgtac cgcggttccg aaccgtcggg
     3601 tgcggcacca ccacgcaacc gttcggtgaa cgggacgggc cgccgcggtt tctccgtcgg
     3661 gcaggggcga gatcgcgtca catccgcggt gcgccggggt cgacaccacc gacaaccgca
     3721 ggaagttgcg cgaggtggga cggctccgcc gggaaccgaa cggccgacaa gtcctgctca
     3781 tccccacagc gggagacgcg gggcgacggt ccgtccgatt ggcgaccggc cgaaactcga
     3841 cacttggtct agaccttgac aggtagtgcg agttccggtt tggttccctc acccccatgg
     3901 cggccgtcgc tcaacgacgg ccgcgcgaag gagtgagtcg tttgcgacag cgaattcttg
     3961 cactgttcac cgcgctggtc tcggccatgg ccctcgcggt attcctgccc gcctcgacct
     4021 cggcggcggc cacgtcggcc gccgcgtgta ccaccggatg gaacgcgtcc acggtctact
     4081 ggggtggcga caccgcctcg tacgacggcc gcaattggac ggcgaagtgg tggacccaga
     4141 acgagagacc cggcacggtc gacgtctggg ccgacaacgg cgcctgcggt ggtggcccga
     4201 ccgacccgcc gcccgccggt ggcttcgtgg tcagcgaggc gcagttcaac cagatgttcc
     4261 cgagccgtaa ttccttctac acctacaacg gtctcaaggc cgcgctgagc gcctacccgg
     4321 ccttcgcgac caccggcagc gacaccatca agcgccagga ggccgcggcc ttcctcgcca
     4381 acgtcagcca cgaaaccggc ggcctggtcc acatcgtgga gcagaacacg gccaactacc
     4441 cgcactactg cgacagttcc cagtggtacg gctgcccggc cggccaggcc gcgtactacg
     4501 gccgcggacc catccagctc agctggaact tcaactacaa ggccgcgggc gacgccctcg
     4561 gcatcgacct gctcggcaac ccctggctcg tccagaacga cgcggccgtc gcctggaaga
     4621 ccggcctctg gtactggaac acccagaacg gtccgggcac catgaccccg cacaacgcga
     4681 tggtcaacag ccgcggattc ggcgagacca tccgcagcat caacggcagt ctcgaatgca
     4741 acggaggcaa ccccgcacag gtccagagcc ggatcgccaa gtaccagagc ttcgtccaga
     4801 tcctcggcac cacccccggc ggcaacctcg gctgctgagt ccggagcgtc actacgacaa
     4861 tcggacgtag ttcagagaac tgttctgcga cctcctcgtt gaccggttcg gggcagagac
     4921 ccgtctgccc cggaccggat cagtgtccgt gccccgccgc ccgcgagcgg tggggcacgg
     4981 cggacgagtg gaggttcgcc atgccaggcg gtacccagtc gcagtcgcgg cccgtgcagg
     5041 cgcgccctgc ggaacccgcg gcccccgcgc caccatgccc gcgccatgac ccgcccgtat
     5101 cgcgggcgcc cgccccaccg cctccgccgt cgctgccgcc cagccgctcc cggcgtctct
     5161 tcctgcgcgc ggctttcgcc ctcggagtga tcggcggtac ggcgacggcc ctgctgcccg
     5221 tcctccgcgc ggacccccgt accgccggtg ctccgcccgc cgaggcagcg ggcccaccgc
     5281 cggtaccggc gaagggggcc gagctgttcg acgagatgta cggcggccgg cacatccagg
     5341 ggacggacgc gggcgtcctc atcgatggcc ggccgctgca cgtcatgcgg cgcgccgacg
     5401 gcagccatct cagtgtggtc aaccactacg agtcgttccc gaccgccctc gcgaccgccc
     5461 gcgcggcggt cgacgacctc ggcggtgccc agctctcgct cgccggaccg gcattccact
     5521 gacccgcagg gaggaccctc cacggtgcac atcagaaaga atcagcgcaa tctcaccagt
     5581 gcggagaaga agcgcttcgt cgccacggtc ctggagctca aacgacaggg ccgctacgac
     5641 gagttcgtcc gccagcatgt ccagtactac cgggccgacg gcgacgaggg gctgcgggcc
     5701 gcgcacatgg cgccgtcgtt cctgccgtgg caccgcaagt acctgctgga gttcgagcgg
     5761 gcgctccagc gcatcgaccc caaggtgacc gtgccgtact gggactggac gacggacaac
     5821 acacccgccg cgtccctgtg gtcggaggac ttcctcggcg gcgacggccg ttcctccgac
     5881 gggcaggtca cgaccggggc gttcgcctac ggggcgggca actggaccat cgacgacggg
     5941 gtgctggagc ggaagttcct ggtccgcgcc ttcggccgtc ccacctcacc gatcgcgctg
     6001 cccaccaagg ccgagctggc ctgggcgatg gacgagtcgg tctacgacgc tgcgccctgg
     6061 gactcgactg ccccgcgcgg cttccgcaat cgcctcgaag gctggaccgc ggccagcgac
     6121 aagacgcgtt ggcgtaatca caaccgtgtc caccgatggg tgggcggcca gatgctgggc
     6181 gcgacggcgc cgcacgaccc ggtcttctgg ctccaccacg ccttcgtcga cctcctctgg
     6241 gaccgctggc agaagaagca cccgaaggcg acctatctcc cgcagcggaa gctgggcggc
     6301 ggcgatcccc agcgcggaca ggcgatcagc gtggacgagc cgatgccgcc gtggcggacg
     6361 aagccgtcgg agatgctcag ccaccggagt ctgtaccggt acgaggcctg agcaggggtt
     6421 cgcgcgggcg cgcccccggg cgcctcgcgc cgaccccgcc cgcacacctg gttccgcacg
     6481 cacgacggcc tcccgcatcg gatgcgggag gccgtcgttt cacgcctcag tcgcgggcgg
     6541 ggacgtatgc ccccgggcgg cggaggttca gcggccgtag ccgccgctgt ggtgcttggc
     6601 gtcggcgttg acgcactggt tgccgaacgc cgggttcagc aggccgatca cgtcgatggt
     6661 gttgccgcag aggttcaccg ggacgtgcac gggcacctgg accacgttgc cggaaaggac
     6721 tccgggcgaa ccggcggcca cagcctcggc gccggcgtcg gcagcggcca taccggcacc
     6781 gccggccagc acggcgcccg tgccggcgag aacagcagct gccttcgcaa tacgcgacat
     6841 caagtttctc cttgaacgtt tcgagcacag ccgcaccatt cgcggccttc acgcacgttc
     6901 aacgcgcaaa ctcacccccg gtaacgaaat tgatccaatc ggtgaggaaa tgccatgcga
     6961 caaaggggtg cacgcgtgtt taccctcggg gtgaacgctt gtgcacccgg ggccgtacgg
     7021 ggtcacggac gcctccacac cgcagacgac cggagagcag atgtccgcag cagcaatacc
     7081 ggagcccgga ccggcaccgg cactcgaacc cgtggagggc cggcgccgcc gcttcctcgt
     7141 cgcggtgctc gccttctgcg gagtagtggt cgccgtcatg cagacgctgg tcgtaccgct
     7201 gctgccgcac gtgcccgccc tgacgggcag cacgccggcg gcagcgagct ggctcgtcac
     7261 gatcacgctc ctgaccggcg ccgtcttcac ccccgtgctc ggccgtgtcg gcgacatgta
     7321 cggcaagcgc agggtcctca tcgcctcgct gggtgtcctc gtcgccggtt cggtcatgtg
     7381 cgccgtcagc tcccacatcg gtgtgctgat cgccggccgg gcactccagg gcgccgcgct
     7441 cgccgtcgtt ccgctcggca tcagcatcat gcgcgacgag ctgccgcccg cacggctgct
     7501 gtcctccgtc gccctcatga gctcgaccct cgggatcggc gccgccgtag gtctgccggt
     7561 cgccgccctc gtcgtccaga acttcgactg gcacaccatg ttctgggcct ccgccgtgct
     7621 gggactgatc gacatcgtgc tcgtcctgtg ctgcgtcccg gagtcacccc tgcgctcccg
     7681 aggccggttc gacgtcatcg gcaccctcgg cctgtccgcc accctcgtca gcctgctcct
     7741 cgccgtcacc cagggcgcgg actggggctg gacctccgcc cgcaccgtcg gactgctcgg
     7801 ggccgcactg accatcggtc tgctctgggg cgcgtacgag ctgcgcacca cgtctcccgt
     7861 ggtcgacctg cgggtctccg cgcgccccgc cgtactgctc acgaacatcg cggccctcct
     7921 cacaggcttc gccttctacg cgaactccct ggtcacggcg cagatggtgc aggagcccac
     7981 gagcaccggt tacggaatcg gcgcatcgat cgtcgtgggc ggactgtgtc tgctgcccgg
     8041 tggtctcgcg atggtggcgc tgtcgccggt atccgcacgg atctccgcga agtacggccc
     8101 caagacggcc ctggcgctcg ccgccgcggt gatggccgtc gggtacgtgg tgcgcttctt
     8161 caccagtcac agcctgtggc cgatcatcgg cggcgcgacc gtcgtcgcgg cgggcaccgc
     8221 gatcgcctat tcggccctgc ccgcgctcgt catgcgggcg gtaccggcgg gcgaaaccgg
     8281 agcggcgaac ggactcaaca ccctgatgcg gtccgtgggg caggccttct gcagcgcggt
     8341 ggtcgccgcc gtgctggcca acatcacctt ccaggcgggc ggccgtaccg cgcccacgct
     8401 ccacgcctat ctgctggtct tcctcatcgc ggccggcgcg gccctgctcg ccctggcggt
     8461 caccctgtgc ctgccgggcc gcgcggtggc ggccgaggcg ggtaccgtcg gcaaggagca
     8521 gacagcaagc cgtacgttcg ccgaggaggg cgcgtgacga ccggacatgc ggccgacggg
     8581 acgacggtcc cggggacgga cgcgggcgtc ggccccgacc ccgaccccga ccccgacccc
     8641 gaccccgacc ccggccccga ccccgacccc gaccccgacc ccgaccccga ccccgacccc
     8701 ggccccggcc gtctcgcgat cctgcgcgcc gcgcgccgcg cgttcaccca gcgcccgtac
     8761 gccgaggtga ccatgcgcgg catcgccgcc gacgccggcg tcagcgcctc gctcatcgtc
     8821 aaacgcttcg gcacgaagga acggctgttc aacaccgtcg ccgacttcgg cccggccgcc
     8881 gacaaactct tcgccgcgcc ccccgccgtc ctcggcaggc acctggtgat caccatggtc
     8941 cggctgcggc gcgagaacca ctcggacccc ctgctgcgcg tcgtcttctc cctcggcaac
     9001 atggacgagc ggaccctgct gcgcgagcgc ttccgcgagc aggtcaccgc ccggctggcc
     9061 ggcctcatcg gcggtgagcg gagtgagctg agggcggagc tgatcaccgg ccagctcctc
     9121 ggtctgggcg ccaccctcag cctccaccgc ccgggtgcgg gggaggaggc cactgaagaa
     9181 ctgctggcgg acctctacgc ccccgcgctc cagcggctga tcaccggaga gcccgaccac
     9241 cccgaccagg ggcccggccg ggccggccgc tgagcgtcgg cgcgagcccg gcacgcccgt
     9301 ccgcttgcgg tcggtgccgg tgccgcgcac acaattgaca tcagaaggca cagaacgaac
     9361 cgacccgccg tgcccgcgaa tgggaagagg ggcccgtgag tgtcgacccg ccatccggcc
     9421 ttccgctccc cgaagggtcg gagccggact ggcccgccgg acccggcctc accctcgcgt
     9481 acaaccgtat gggcagcttc gacatcgatt tcgtccgcga gtacatgcac ctcgaccccg
     9541 gtgccctgga ggtgttcgat ctccgccccg acgaacacga cggcagcatg gaggtcccca
     9601 gccgggtgcc gcccgaggaa ctggaacgcc tggacacact gatccagcgg gcgctggaca
     9661 agggtggcag catctacggc gcgtacttct ggatccactg ccgcgacggg tcacgccgct
     9721 ggctccacac ccagggccat gtcctgcgcg actcggaggg ccggccccgc cgggccatcg
     9781 gcatcgtgcg cgacgcgacc cgggaactgg acgacgcggc caagcgcacc gagatggtcg
     9841 agacccgccg ccggctcacc gacatggtcc gccgggtcac cgccctgctg gcccgtgcga
     9901 ccaccgtgcg ggacgtcacc gacgcgctca aggacgccga ggcactgagc aacctcggcg
     9961 cggtgagcgt gatgctcggc atcgtcgaga acggacatgt gcacacggtc accgaggggc
    10021 ggctgggtct gtacgtcccc gagctgcagt acacacggat cgacgcgcgg ttcccgatga
    10081 gcgaggtcgt ccgcacgcta cggccccggt acatcacgtc acgtgaggag ttccggcgct
    10141 ccttcccccc gctgtggcac cacatccggc atctcccggt ggcctcgggc gcctatctcc
    10201 cgctgatcgt gcagggcagg acgatcggcg cgctcggaat gctgtatcca cggcacggcg
    10261 gcttcagcgg cgaggaacgc aacctgctgg tcacgctcag cagcagcatc gctcagagcc
    10321 tccagcgcgc catgctcttc gagcaggagc acgatctcgc cgacaccctg caacgcgcca
    10381 tgctgccgca tcacatcccc gaggtccccg gcgcccggat cgccgtccgc taccgcgcgg
    10441 ccagggccgg tcgtgacgtc ggcggcgact ggtacgacgt ggtcccgctg cccggcggcc
    10501 gggtcggact gatgatcggc ga
//
