LOCUS       NZ_CP048397            10228 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. S4.7 chromosome, complete genome.
ACCESSION   NZ_CP048397
VERSION     NZ_CP048397.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. S4.7
  ORGANISM  Streptomyces sp. S4.7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7920066)
  AUTHORS   Bekiesch,P., Zehl,M., Domingo-Contreras,E., Martin,J.,
            Perez-Victoria,I., Reyes,F., Kaplan,A., Ruckert,C., Busche,T.,
            Kalinowski,J. and Zotchev,S.
  TITLE     Viennamycins: new lipopeptides produced by Streptomyces sp. S4.7
            isolated from the rhizosphere of Edelweiss (Leontopodium nivale
            subsp. alpinum)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7920066)
  AUTHORS   Bekiesch,P., Zehl,M., Domingo-Contreras,E., Martin,J.,
            Perez-Victoria,I., Reyes,F., Kaplan,A., Ruckert,C., Busche,T.,
            Kalinowski,J. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2020) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.8x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010384365.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 10:27:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,027
            CDSs (total)                      :: 6,939
            Genes (coding)                    :: 6,650
            CDSs (with protein)               :: 6,650
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 103 of 289
            Pseudo Genes (incomplete)         :: 223 of 289
            Pseudo Genes (internal stop)      :: 23 of 289
            Pseudo Genes (multiple problems)  :: 52 of 289
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6674664
            Orig. end                         :: 6684892
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048397.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zotchev Lab, University of
            Vienna, Austria.
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10228
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="21"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10228
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10228
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5228](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5228)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(988..1003)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.38"
                     /tool="antismash"
     gene            complement(1660..3780)
                     /locus_tag="SSPS47_RS29635"
                     /old_locus_tag="SSPS47_30045"
     CDS             complement(1660..3780)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510298.1"
                     /locus_tag="SSPS47_RS29635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_30045"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_239065352.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.8e-34, bitscore:
                     116.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEALECGAASLAMVLAHYGRHVPLEELRIACGVSRDGSRASNVLK
                     AARGYGLQAKGMQMETEALADVRAPAILFWEFNHYVVYDGMGRRPAGRGVHINDPDKGR
                     RFVPIEDFDTSFTGVVLVFEPTDDFRRGGRRPGVMSAMPARMRGTAATLLAALLASLLL
                     VAVGAAVPALSRTYIDMFLIGGQTSLLGPLFASMAVMLALTAVLTGLQQANLLRGRIIS
                     STLGGARFMRHLLRLPVTFFAQRSPADLVQRLQSNDAVAETLARDLAAAGVDGVVVILY
                     AALLWSYDPQLTVVGVLMALLNVIAMRIVIHLRSTGTRKLRADSARLTNTSYTGLQLIE
                     TMKATGGENGYFRRWAGQHATTLEEQQKLGVPSAALAVVAPTLATLNSALILWIGGLRA
                     VEGAISIGLLVAFQALVVRFTAPITRLNGVAGRIQDFAADVARLKDVESFPVDPLYSHS
                     RPDAGTRRLTGHVELESVVFGYSPLDAPLLNGFSLTVGPGQQVALVGGSGSGKSTVSRL
                     ISGLYSPWEGTIRIDGRRLEDISRSALASSVSFVDQDVFLFEGTVRDNVALWDPSISDD
                     AVVTALRDAALYDDVIARRPDGIHGRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDE
                     VTSALDAETERLIIDNLRRRGCACVVIAHRLSTVRDSDEIVVLDHGAVVERGRHEDLVA
                     AGGPYAQLVKEY"
     gene            complement(3993..4799)
                     /locus_tag="SSPS47_RS29640"
                     /old_locus_tag="SSPS47_30050"
     CDS             complement(3993..4799)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="SSPS47_RS29640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_30050"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_164253616.1"
                     /transl_table=11
                     /translation="MQFRQQALSRLQSPEEIDLPVRLARPQGCLVLAVTVVAMIAAAVW
                     AVTGTVSSTLGAPGILTYAQGSYVLQSPVAGLVTDVLAEEGERVPADSPLVKVRTPEGD
                     TVVRTIAAGRLTTLVAAVGSVITTGADVAAVERIEKDDDPLSVMLYAPAESGATVPVGA
                     SVDVTVQSVPKQRYGVLRGKVEAVGRVAETSRRITGFLGSTQLGEQFSREGPPVAVLVR
                     LDESSATRSGYSWSSQDGPPYAVESMTLATGSVTLAEQHPIDWLLP"
     gene            complement(5001..5228)
                     /locus_tag="SSPS47_RS29645"
                     /old_locus_tag="SSPS47_30055"
     CDS             complement(5001..5228)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157700.1"
                     /locus_tag="SSPS47_RS29645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_30055"
                     /product="type A2 lantipeptide"
                     /protein_id="WP_147876380.1"
                     /sec_met_domain="strepbact (E-value: 3.6e-31, bitscore:
                     106.1, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRSPQVETQEISDADLDNVSGGLVGGLVGTVTGTVDSIVPVSST
                     VGGVVGTVEGATGIDTSGVTGLATGVVSSL"
     misc_feature    5224..5238
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.32"
                     /tool="antismash"
     misc_feature    complement(5245..5258)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.94"
                     /tool="antismash"
     gene            5261..5515
                     /locus_tag="SSPS47_RS29650"
     CDS             5261..5515
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SSPS47_RS29650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_164253617.1"
                     /transl_table=11
                     /translation="MATTGFAVLAAPVVAAVVEALLRISEYQDSEIRRMAPGDFPRCEG
                     SKHACLSSSHSKAERTDRTVELQRAEVPGRPWLGGIFFT"
     misc_feature    complement(5606..5624)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.2"
                     /tool="antismash"
     misc_feature    complement(5764..5773)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(6145..6159)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.79"
                     /tool="antismash"
     gene            6183..6677
                     /locus_tag="SSPS47_RS36310"
     CDS             6183..6677
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /locus_tag="SSPS47_RS36310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="WP_343234908.1"
                     /transl_table=11
                     /translation="MILEYIRYRISENRAAEFEAAYARAAGPLGRSPFCHDFELSRCVE
                     ESDHYILRIRWESLDKHLKDFRSSAEFRAFFTGIRPYVDAIEEMRHCTPTEVAGVGAGT
                     PRPPTLYEWAGGGDAFDRLSSRFYEIVATDELLAPMFSGMSADHPHHVALWGGEVFGGP
                     A"
     gene            6708..6953
                     /locus_tag="SSPS47_RS36315"
     CDS             6708..6953
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SSPS47_RS36315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_343234909.1"
                     /transl_table=11
                     /translation="MLAHHIGKAITPRQRRRWVDLLADAADDVGLPADPEFRAAFTGYL
                     EWGTRIALENSRPDAAPVPRAPVPRWGWGEAPPYRP"
     gene            7070..8389
                     /locus_tag="SSPS47_RS29660"
                     /old_locus_tag="SSPS47_30070"
     CDS             7070..8389
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313254.1"
                     /locus_tag="SSPS47_RS29660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_30070"
                     /product="hypothetical protein"
                     /protein_id="WP_164253618.1"
                     /transl_table=11
                     /translation="MTDTGPTLETVTGARQTLTVVLPVRLHRTPDPRAGWAQGPYPFTQ
                     GARRTDTATRSGYFAPASARVLYGTPDRPCRWHRVLAVTHDGLHLIGLEILRTATATAT
                     ATARDPRHALAVLHFTVDVPLLPVLRAIGHRPTAGPDPLSGPLDPDTLLDAVAEVRDRT
                     GTFALARPYTVAFLTPGAHHTPALRPDPEAMLPPTADRWLWQLASRSAPGDFPVVPESA
                     PHHEASTVRISADWSALVLRHGAAFLGHRADGGDGDFYDFGALHARTVYLDALLLGSLQ
                     RDHIDELTEELSEVFDSERLTRRVTALEKNIARFRSTYWRQHLTAHGPANGLLRAFQHQ
                     HRLPERVAEILAEAADYSRLVQTLESQQISGALGVLTILGLPLGTALSVLQVLGDESVP
                     HLLVALGLSVAATAAALTTRYGRLVLSSLRGGTTAPDRRR"
ORIGIN
        1 acccggcgga tgccgctgac gcccatcgcc gcgctggcca gctcaccggt ggagcgttcg
       61 gcgaagaacg ccgtcggcag ccgcaacaac cggtcccaca cggcgggttg cagcgagctc
      121 tcgatacggc cctccatccg caggatggtc aggctctgca tcagcatgaa ggaggccgac
      181 acgatgccgg tgatcatgac ggcgagtgac acctgcacga tcggaccggt ctgcgcgctc
      241 ggtacgtagt cgcccagcac ccgccccgtg gcgatcggca ccagcgcgcc gagccccacg
      301 gtgacgagcg tggtcagcag gaggttgcgc aggtcggtcc ggttgccgcg cagcccgaag
      361 cgcagcaacc gccaaggcgt caggggctct tcgggcagcg ggcggtagaa catcacggcg
      421 cgcggctcga agtcgtcggc gttccgctcg cccacacgca gccgcgcccc ggtggccggg
      481 ttgaccgcct cgtaacggcc gcgccgccac agcagcgcca ccggggcgcc ggacttggcc
      541 cggtggccga ccaacggtcc ggagtcggtc cgccaccagt gggcgtcgag ccggacggcg
      601 cgggtacgga cccgggaggc gatcgcgatc cgctcgaccg gttcgacacg gtcgtcgagc
      661 ggggtacccg cgccacggga gtggtcggag agggcgatac cggaggccgc ggcgaccaga
      721 cggcagacgg cgaacgtggc gtcgcgggcg gcgtcgtccg tcaccgcgtc ggccgtgccc
      781 ccggcgcggc ccgtgccgcc ggtccctgcc cccgggcggc cgatggacgc caccagcgcc
      841 tcgtccgcgc gtgcccggac cgcttccccg gccttgatac cggcggcggt gcggtcctcg
      901 tgcgcgcgct cctgccgctc gatccagtgg tccacggccg acagcagccg gtgctgctga
      961 ttgaccatcc gctgccacat ggccccgtcg accagcaggt cacccgccgc gtccgcgctg
     1021 tacgaggcgc cgtaccgcac actgccgggg gacaccggca tccacagcac gtcgtcgtcg
     1081 gacacggcgc cgtcggcgtg cgggcgggtg tcgagcgggg tctggaagag cgcgccgagc
     1141 ccgcgggcgg cgccgagggc gaaggcgtgc tccaggggcg tcgggaccgg gggagagggc
     1201 ggcgaggtgt ggtcgctgcc gtacgggtcg ccgtacgcgt ccccgtactg gggcgagtaa
     1261 ggatccgggt acgcgggcgc gtacagctcc cgcaacgcga tccgccgcag cgcacagtcc
     1321 tgcgaaggcc tgctcagcaa ggtgtgccgg gggccctcga ccggaccgag gagcagcgtt
     1381 cccgcttcga ggcggccgag gaagtgccac tggccgcgct gcgcggcgtc gacggcgaac
     1441 aggtcgagcg cgccccagac gacgagccac agcacatgcg ggccctccag cggcacgttg
     1501 cgcagaccgg cgcagtcgac cggggtaccg agcgcgccga gggcgtggac gaccggatcc
     1561 atgccgggcg ccgggcccgc cgccgtccca ccggtggctc cggccgtcgc gtcgcccgcc
     1621 gtcccggggc cggccggtgg tggctgcgtg gaggccacgt cagtactcct tgaccagttg
     1681 ggcgtacgga ccaccggcgg cgaccaggtc ctcgtgcctg ccgcgctcca cgaccgcgcc
     1741 gtggtcgagc accacgatct cgtcgctgtc gcgcaccgtg ctcagccggt gcgcgatcac
     1801 cacgcacgcg cagccgcgcc gccgcaggtt gtcgatgatc aggcgctccg tctccgcgtc
     1861 cagcgcgctc gtcacctcgt cgaggaccag gatgctcggc cgcctgacca acgcgcgcgc
     1921 gatctccagc cgttggcgct ggccgccgga gaagttgcgg ccgtcctgct cgaccctgcc
     1981 gtggatcccg tcggggcggc gcgcgatcac gtcgtcgtac agcgcggcgt ccctgagcgc
     2041 ggtgacgacg gcgtcgtccg atatcgaggg gtcccacagc gcgacgttgt cccggaccgt
     2101 gccctcgaag aggaagacgt cctggtcgac gaaggacacc gaggaggcga gcgcgctgcg
     2161 cgagatgtcc tccagacgcc ggccgtcgat gcggatcgtc ccctcccacg ggctgtagag
     2221 acccgaaatc agcctggaaa cggtggactt gccgctcccc gaaccgccga ccagcgcgac
     2281 ctgctgcccc ggacccaccg tcagcgagaa gccgttcagc agcggcgcgt ccagcgggct
     2341 gtagccgaac acgacgctct ccagctccac atggcccgtc agccggcggg taccggcgtc
     2401 cggccggctg tgcgagtaga gcgggtcgac ggggaagctc tcgacgtcct tcagccgggc
     2461 cacgtccgcg gcgaagtcct ggatccggcc cgccaccccg ttcagccggg tgatcggcgc
     2521 ggtgaaccgc accaccagcg cctggaacgc gacgagcaga ccgatcgaga tggccccctc
     2581 caccgcccgc agcccgccga tccacagaat cagcgcgctg ttgagcgtgg cgagagtcgg
     2641 cgccaccacc gccagcgccg cgctgggcac accgagcttc tgctgctcct ccagagtcgt
     2701 cgcgtgctgc ccggcccacc ggcggaagta cccgttctcg ccgccggtcg ccttcatcgt
     2761 ctcgatcagc tgaaggccgg tgtacgaggt gttggtcagc cgcgcgctgt ccgcccggag
     2821 tttgcgtgta ccggtggagc gcagatggat cacgatccgc atcgcgatca cgttgagcag
     2881 cgccatcagt acgccgacga cggtgagttg ggggtcgtag gaccacagca gcgcggcgta
     2941 caggatcacc acgaccccgt ccacaccggc cgccgccagg tcccgcgcca gtgtctcggc
     3001 caccgcgtcg ttggactgga gccgctggac gagatcggcg gggcttcgct gggcgaagaa
     3061 cgtgaccggc aaccgcagca gatgccgcat gaaccgggcg ccgccgaggg tggaggagat
     3121 gatgcgcccg cgcagcaggt tcgcctgctg gagcccggtc agtacggcgg tgagcgcgag
     3181 catcacggcc atcgacgcga acagcggtcc cagcagcgat gtctgaccgc cgatcaggaa
     3241 catgtcgatg tacgtacggc tcagcgcggg caccgccgcg cccaccgcca ccagcagcag
     3301 gctggcgagc agcgcggcca gcaaggtggc ggccgtgccg cgcatccgcg cgggcatggc
     3361 gctcatgacg ccgggcctac ggccgccccg gcggaagtcg tcggtgggct cgaagacgag
     3421 gaccacaccc gtgaagctgg tgtcgaagtc ctcgatcggc acgaatcgcc ggcccttgtc
     3481 ggggtcgttg atgtgcacgc cccggccggc cgggcggcgg cccatgccgt cgtagacgac
     3541 gtagtggttg aactcccaga acaggatcgc gggcgcgcgg acatcggcca gtgcctcggt
     3601 ctccatctgc atgcccttgg cctggagacc gtaaccgcgc gccgctttga ggacgttgga
     3661 ggcgcgcgag ccgtcgcgtg agacaccgca cgcgatgcgc agctcctcca ggggaacgtg
     3721 gcggccgtag tgggcgagca ccatggcgag ggaggccgcg ccgcactcca gggcctccat
     3781 ctggaggacg gtgggggtgc ggacggtcct gggccccggg gctccggcgg cgggccggcc
     3841 gcgtttggcg gctcggcgcc ctccgccgcc ggtgcccgtg cccgtacgcg aggcgtcgcc
     3901 gggtgtctcg tcgggccggt gcccgcggcg ccctgcgggc ggcagccgct gctgcttgcg
     3961 aggggccggg ggaagctggt gccggcgcgg gctcacggga gcagccaatc gatcgggtgc
     4021 tgttcggcca gcgttacgga accggtggcc agggtcatgg attcgacggc gtacggcggc
     4081 ccgtcctgcg acgaccagga gtagccggac ctggtggccg acgactcgtc aagccgcacc
     4141 agtacggcca cgggtggccc ttcgcgcgag aactgctcgc ccaactgggt gctgccgagg
     4201 aagccggtga tgcgccggct ggtctcggcg acgcggccca cggcttccac cttcccgcgc
     4261 agcacgccgt agcgctgctt gggcaccgac tggacggtga cgtccacgga cgcgccgacg
     4321 ggcaccgtcg cgccgctctc ggcgggcgcg tacagcatca cgctcagcgg gtcgtcatcc
     4381 ttctcgatcc gttccaccgc cgcgacatcg gcacctgtgg tgatcacgga gccgaccgcg
     4441 gccaccaggg tcgtcagccg gcccgccgcg atggtgcgga cgacggtgtc gccctcggga
     4501 gtgcggacct tgacgagggg ggagtcggcg ggcacccgct cgccctcctc cgcgagcacg
     4561 tccgtgacca ggccggcgac ggggctctgg aggacgtaac tgccctgcgc gtaagtgagg
     4621 atgccggggg cgccgagggt ggacgacacg gtgcccgtca ccgcccagac ggccgcggcg
     4681 atcatggcga ccacggtgac ggccagcacg agacagccct ggggccgggc gaggcgcacc
     4741 ggcagatcga tctcctcggg cgactggagc ctggaaaggg cctgttgacg gaactgcacg
     4801 acgctcttcc ctggtagctc tggtagctct ggtagctctg gtagcccggt gttcccggcg
     4861 cgcacggtcg gacggctccc ggccggacgg cgcacggccg ttccccacgt cccacgttct
     4921 gtgtcccacg accagcgccg cgccggccgt gtgcccggta cccgggcgca cggccgacgc
     4981 ggctgagccg acgtctcaac tcagaggctg gagaccacgc cggtggcgag accggtcacg
     5041 cccgacgtgt cgatgccggt ggcaccctcg acggtgccga cgacgccgcc aaccgtgctg
     5101 gagaccggga cgatggagtc gacggtgccg gtgacggtgc cgacgaggcc cccgacgaga
     5161 ccgcccgaga cgttgtcgag gtcggcgtcg gagatttcct gggtctcaac ctggggagag
     5221 cggttcataa tgtgagattc ccttcgcatg gctattcaca atggccacga ccggcttcgc
     5281 cgtactggcc gcaccggtcg tcgcagcggt cgtcgaggcg ctgctacgta tttccgagta
     5341 tcaggattcg gaaatccgtc gaatggcgcc gggtgacttc ccgcggtgcg aaggatcaaa
     5401 gcacgcatgc ctgtcgagta gccactcgaa ggccgaacgc acggaccgga cagtggagtt
     5461 gcagcgcgcc gaagtgcccg gccgtccatg gcttggtggc atcttcttca cgtgatcgac
     5521 aaacccgaac tggacattcc cccaccaaag cgattaccgg cggggtgtcg caggtacgta
     5581 cctggcggag ggccgtgccc acctgcgtat tcggtgtgca gattccccgc agaaggcaac
     5641 cgcccgaaag tccgccgccg gccgcgccga ttgcctcgtc gcggtatttc cggggaaacg
     5701 gcgcggcttc caggagaagg caacataaac gcataacgcc gttgcgccgg acgcggggga
     5761 aatggccgcc ttccgtccgg tcgccgcccc gttcgacgcg ttcggtgcgg tctgtcgtcc
     5821 ggtgtcctga atcgatgatt ccgccgggtc tgcgccgggc ggatcgtgag cctcgggcgt
     5881 gagcgcgtac tcgacgcgcg ggctgggtgg cacgcgccac cgcacccgcg acggtgccgc
     5941 gttgtccctg ttcgcggagg tgccgcggcg gcctcttctc cgtttcgccg tgatgtcgcg
     6001 caccggccgc cggatccgcg aaaagcggag gagctcattc tcctcggggt gcgccggtac
     6061 caccggcgat tacccatccg tgtcgccgag cggatccggc gacacggatg acaggcaccc
     6121 ataagcgggt gccccggcac ccggaaatat ctgtgtattc tccctgccgg aggtggggac
     6181 acatgatcct cgaatacatt cgctaccgaa tttccgagaa ccgcgcggcg gaattcgaag
     6241 ccgcctacgc ccgcgcggcc ggcccactgg gccgctcgcc cttctgtcac gacttcgagc
     6301 tctcgcgctg tgtcgaggag tccgaccact acatcctccg gatccgctgg gagtcgctcg
     6361 acaagcatct gaaggacttc cggtccagtg cggagttccg tgccttcttc accgggatcc
     6421 gcccctacgt cgacgcgatc gaagagatgc ggcactgcac cccgaccgaa gtcgccggag
     6481 tcggcgccgg tacaccgcgg ccaccgaccc tctacgaatg ggccggtggc ggcgatgcct
     6541 tcgaccggct ttccagccgc ttctacgaaa tcgtcgccac ggacgaattg ctcgcgccga
     6601 tgttttccgg catgagcgcg gaccatccgc accacgtggc gctctggggc ggcgaggtgt
     6661 tcggcggccc cgcctaatac acggctcacc acggcggtta cgaggccatg ctcgcccacc
     6721 acatcggcaa ggccatcacc ccgcggcagc gcagacgctg ggtggacctc ctcgccgacg
     6781 cggcggacga cgtcggcctc cccgccgacc cggaattccg cgccgccttc acgggctacc
     6841 tcgaatgggg cacccgtatc gccctggaga actcccgccc cgacgcggcc ccggtacccc
     6901 gcgccccggt cccgcgctgg ggctggggag aggcgccgcc gtaccggccg tgatcccgtg
     6961 acaccggccc ccgccacccc aactgccgcg cggtaacctc ctgatgaccg accggaggaa
     7021 ccccgaggag gaccgtggag cggcgccacg agcgagggcg gcggcagcga tgaccgacac
     7081 cggccccact ctcgaaaccg tcaccggcgc acgccagacg ctcaccgtcg tcctcccggt
     7141 ccggctgcac cgcacacccg atccgcgcgc cgggtgggcc cagggtccgt atcccttcac
     7201 ccaaggcgcc cgccgcaccg acaccgccac ccggtccgga tacttcgcgc cggcctccgc
     7261 ccgcgttctc tacgggacac cggaccgccc ctgccgctgg caccgggtgc tcgcggtcac
     7321 ccacgacggc ctgcacctca tcggcctgga gatcctgcgc accgccaccg ccaccgccac
     7381 cgccaccgcc cgcgaccccc ggcacgccct cgccgtactg cacttcaccg tcgacgtacc
     7441 actactcccg gtgctgcggg ccatcggcca ccgccccacc gccggacccg accccctcag
     7501 cggccccctc gaccccgaca cccttctcga cgcggtcgcc gaagtacgcg acaggacagg
     7561 tacgttcgcc ctcgcccgtc cctacaccgt cgccttcctg acccccgggg cccaccacac
     7621 accggcactg cgcccggacc ccgaagcgat gctgccgccc accgccgacc ggtggctctg
     7681 gcagctcgcc tcccgctccg ccccgggcga ctttcccgtg gtacccgaga gcgccccgca
     7741 ccacgaagcc tccaccgtgc ggatatccgc cgactggagc gccctcgtac tgcgccacgg
     7801 cgccgcgttc ctcggacacc gggccgacgg cggggacggc gacttctacg acttcggcgc
     7861 cctgcacgcc cgcaccgtct acctcgacgc gctgctcctc gggtcgctcc agcgcgacca
     7921 catcgacgaa ctcaccgaag agctctccga ggtcttcgac tccgaacgcc tcacccgccg
     7981 ggtgaccgcg ctggagaaga acatcgcccg cttccgcagc acctactggc gccagcacct
     8041 caccgcacac ggtcccgcca acggcctgct ccgcgccttc cagcaccagc accggctccc
     8101 cgagcgcgtc gccgagatcc tcgccgaggc cgccgactac agccggctgg tccagaccct
     8161 ggagagccag cagatcagcg gcgcgctggg cgtcctcacc atcctcggac tcccgctcgg
     8221 cacggcgctg agtgtccttc aggtcctcgg cgacgagtcc gtcccccatc tcctcgtcgc
     8281 cctcggcctg tcggtcgccg cgacggccgc cgcgctcacc acccgctacg gccggctggt
     8341 cctctcctcc ctccgcggtg gcacaaccgc cccggacagg cgccgctgaa cgcaccggcc
     8401 gcaggccgtc ccacctctgc acgcgatcgg tgcgagtaca cgaacgatca gcatcggccg
     8461 aaaacgttcg aatccgcgct gatccctgcc catccgtagg acgagcggac tagtgtcggg
     8521 acctgacctg cacgtccgtt cacctcaagg agggtgatgg tgcccgcgtc ggagcacccg
     8581 gtcggcatcc cgctcctcga acgcgagccg gagatcgccg ccgccgcgca cgccgtcgac
     8641 gggctcttcg gagcgacacc gtccggcggg ctcctcgtct accgcggcgc cgccgggctc
     8701 ggcaagaccg cgctgctctc cgaggtccgg cgcatggcgg ccggccgctg cacggtgtgg
     8761 tcggcgcgcg gcggcgagac cgtgacatcc gtgcccttcc acgtcgtacg gcagttgctc
     8821 cagccggccc tgacggggcg tcagcccgac gggttacggg aactgctcgg tgacgggtac
     8881 gacatcgtcg gccccgccct cggagtcgca ccgcccggcg aacggcacgc cgatccacag
     8941 ggcgtcaggg acggcttcga gacgctcttc cgctcactcg ccgaagcacg gcccccactc
     9001 gtcctgatca tcgacgacgc gcactggtcc gacctcgaat ccctcgcctg gctggcctcc
     9061 ttcgcccggc gcacgggcgg cccgccgatc ctggtcgtcg tcgcgtaccg cgtcgaggac
     9121 gcgatcggcg agtgcgccca cctcctgcgg gccctgggcg agagcgccag actcctcgtg
     9181 accctgaagg cactcacctc ggaagccacc gccaagctcg cccgcgccag cctcggggag
     9241 cacgccgacg aaccgttctg ccgcgaggtc tgggaagtca ccggcggcaa cgcctacgaa
     9301 accgtcgaac tgctcgccaa ggcgcgcgac caggccctgg accccgtcgc cgagtccgcc
     9361 gacgcactca gcgcgctcgg cgccgcgaca cgcggcaccg gcctcatcgc caggctggag
     9421 gagctgggca ccaccacgac ccgcttcgcc tgggccgccg ccatcctcgg caccgacatc
     9481 tcccccgaac tcgtcgtgtc cctcgccgga atggggcccg cggaggccgc cgactgcgcc
     9541 gagaggctcc ggctggcccg catcctcgtc ggcaccgacc cgctggaatt cgtccacccc
     9601 ctcatcgcca ccgccgtcta ccgcgccatc cctccggcca ccaggacagc cttccacggc
     9661 cgggccgcct ggctcgtcac ccgcagcggc cggggcgcgg ccctcgcctc ccggcacctc
     9721 ctggaggtcc accccgacaa cgacgccgaa ctggtcgagc agttgcgcga ggcagccgcc
     9781 gaacacctcg cggtcggcgc ccccgacgcc gcccgccgat gcctcgaacg cgccctgaag
     9841 gaaccaccgt tgccccgcgt gagcgccgaa gtgctctacg aactgggctg cgccaccctc
     9901 ctgtcgtcac cggccaccac cgtcgatcat ctgcgcgcgg cgatcgacac ccggctcctc
     9961 gacgacgcgc tgcgcgtcga cgcggtcttc cggctgtccc aggccctgac acacaacgac
    10021 cagacccggg aagccgccca ggtcgtcgcc gccgaagccg cccgcaccgc acccgggccc
    10081 gcccagatgc gcctcagggc cgcgcacttc ctctgggagg gcgtccagga cgccgaggac
    10141 gacggcaggg aacgcgcccg ccgcctcacc ggcacggcgg ccggtctcag cggacgcgac
    10201 aacaccgaac gcgtcctgct catgctgc
//
