LOCUS       NZ_CP048397            11410 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. S4.7 chromosome, complete genome.
ACCESSION   NZ_CP048397
VERSION     NZ_CP048397.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. S4.7
  ORGANISM  Streptomyces sp. S4.7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7920066)
  AUTHORS   Bekiesch,P., Zehl,M., Domingo-Contreras,E., Martin,J.,
            Perez-Victoria,I., Reyes,F., Kaplan,A., Ruckert,C., Busche,T.,
            Kalinowski,J. and Zotchev,S.
  TITLE     Viennamycins: new lipopeptides produced by Streptomyces sp. S4.7
            isolated from the rhizosphere of Edelweiss (Leontopodium nivale
            subsp. alpinum)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7920066)
  AUTHORS   Bekiesch,P., Zehl,M., Domingo-Contreras,E., Martin,J.,
            Perez-Victoria,I., Reyes,F., Kaplan,A., Ruckert,C., Busche,T.,
            Kalinowski,J. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2020) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.8x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010384365.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 10:27:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,027
            CDSs (total)                      :: 6,939
            Genes (coding)                    :: 6,650
            CDSs (with protein)               :: 6,650
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 103 of 289
            Pseudo Genes (incomplete)         :: 223 of 289
            Pseudo Genes (internal stop)      :: 23 of 289
            Pseudo Genes (multiple problems)  :: 52 of 289
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6230161
            Orig. end                         :: 6241571
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048397.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zotchev Lab, University of
            Vienna, Austria.
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11410
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="18"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11410
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11410
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6410](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6410)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(259..271)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.29"
                     /tool="antismash"
     gene            501..1553
                     /locus_tag="SSPS47_RS27765"
                     /old_locus_tag="SSPS47_28130"
     CDS             501..1553
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135590.1"
                     /locus_tag="SSPS47_RS27765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_28130"
                     /product="glycosyltransferase"
                     /protein_id="WP_164253337.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.6e-10,
                     bitscore: 39.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKNESRVIGRCLDSVRGLIDSWIISDTGSTDGTQSLIRSALEG
                     IPGELHQDDWVNFGHNRTHNIQHAFGRADYLLLLDADHTVRLESPLPALTGTSYMLRHE
                     GNLEYRVKRLVRGDIDWNYVGATHEYLNTEEEDKQELLDEIVISDFADGGSRHDKFERD
                     AALLADELARNPKNDRAVFYLAQTMRDMGNGAEAIELYERRAMMGGWGEEVYVALLEAG
                     ILRAELGDWPGGMECLTKAWESRPQRLEACYQLSSRLRKMRRHNAAYIFAKAGIGRPVP
                     AGDVLFTNPWVYRWGLLFELSVSSYWIGEYAESVWACDQLLGLADLPEVYRRQTVINRG
                     FSIGRLGARQ"
     misc_feature    complement(508..517)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.04"
                     /tool="antismash"
     gene            complement(2076..3812)
                     /locus_tag="SSPS47_RS27770"
                     /old_locus_tag="SSPS47_28135"
     CDS             complement(2076..3812)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (117-481). E-value:
                     6.1e-20. Score: 63.8. Matches aSDomain:
                     nrpspksdomains_SSPS47_RS27770_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380016.1"
                     /locus_tag="SSPS47_RS27770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_28135"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_164253338.1"
                     /sec_met_domain="PF00561 (E-value: 2.4e-25, bitscore: 88.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVAKYGALATLGSLVLSTLSAAPAGGADFAGTSSEARGTVVAA
                     KRAAAAGITFRACPKAEMLPAPLTCGTVEVPLDYARPDGERIKLTVSRVKASGRTGAAG
                     GDGKAVETVRRQGALVYNPGGPGSSGMAFPLAAKMPEWKRIAAAYDLVGYAPRGVGRSA
                     PLSCQNAGDFAKAPTLSPTYPTESYKRERISRAKAYAQGCARNAGASLRHYTSLNNARD
                     LDVLRAGLGEKKLSFMGASYGTYFGALYASLFPSHVRRMVFDSAVNPDPEQIWYRNNMD
                     QSFAFERRWADFRAWVAKHDATYHLGTTPAEVQRNYERVRAEVAHRPAGGKVGPGQLHA
                     AMLGAGYYDDYWAIRATALSEFLKGNTKPLVDQAAPRPAAAVGNENSNAVYTAVECNDA
                     PWPTDWKVWDRDNTGLAEVAPFETWDNAWMNLPCAFWPAQRRRPVDVRTSAGELPPTLI
                     LAAERDAATPYAGALELQRRLAGSVLVTERGAGTHGIAGGTNECVNDHVEAYLLTGKTP
                     VRRAECAPHAAPNPVSLAEHTELRRRLNSGVRSESVEASLEHGSSSRPTAYTHERRTPA
                     GT"
     aSDomain        complement(2370..3461)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SSPS47_RS27770_Abhydrolase_1.1"
                     /evalue="6.10E-20"
                     /label="SSPS47_RS27770_Abhydrolase_1.1"
                     /locus_tag="SSPS47_RS27770"
                     /protein_end="481"
                     /protein_start="117"
                     /score="63.8"
                     /tool="antismash"
                     /translation="ALVYNPGGPGSSGMAFPLAAKMPEWKRIAAAYDLVGYAPRGVGRS
                     APLSCQNAGDFAKAPTLSPTYPTESYKRERISRAKAYAQGCARNAGASLRHYTSLNNAR
                     DLDVLRAGLGEKKLSFMGASYGTYFGALYASLFPSHVRRMVFDSAVNPDPEQIWYRNNM
                     DQSFAFERRWADFRAWVAKHDATYHLGTTPAEVQRNYERVRAEVAHRPAGGKVGPGQLH
                     AAMLGAGYYDDYWAIRATALSEFLKGNTKPLVDQAAPRPAAAVGNENSNAVYTAVECND
                     APWPTDWKVWDRDNTGLAEVAPFETWDNAWMNLPCAFWPAQRRRPVDVRTSAGELPPTL
                     ILAAERDAATPYAGALELQRRLAG"
     misc_feature    3872..3889
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.77"
                     /tool="antismash"
     gene            complement(4035..4994)
                     /locus_tag="SSPS47_RS36060"
                     /old_locus_tag="SSPS47_28140"
     CDS             complement(4035..4994)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491674.1"
                     /locus_tag="SSPS47_RS36060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_28140"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_203557936.1"
                     /transl_table=11
                     /translation="MDTLITTLQIAIPLSFVTLIVGVITARSSGGTTEVHDLMDVAFLG
                     GGPGRVAETAVVAMHADGRLGIGGPGIVALYSDVARNPVERAVIDEHNRAPHGALHALR
                     LAVMRGRAVQDIGDSLAARGLLVPARKTRVWAVWGLAQAIVCLLAVPLTIVRLISTASP
                     AVIPTLPLAMIGIISGFACAALARRRLTAAGVRARLKFRTAYRHITTPDHQIAVDGHMA
                     LDDPLLREQLTAARGMRPGRDFQPAPHLAATAVVWCASADFGDGGSGGSGCGGSGGSGC
                     GGSGGGCGGGGGSGDGGGGGGGGGCGGGGGCGGGGGGG"
     gene            complement(5001..6410)
                     /locus_tag="SSPS47_RS27780"
                     /old_locus_tag="SSPS47_28145"
     CDS             complement(5001..6410)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175675.1"
                     /locus_tag="SSPS47_RS27780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_28145"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_164253339.1"
                     /sec_met_domain="DUF692 (E-value: 6.3e-97, bitscore: 322.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRTTHVVNVGEVGNARYPGGLRALGIGIGWRPEIAEAVEALPGI
                     DWVEAVAENVCAGHLPDSLVRLRARGVTVIPHGVSLGLGGAARPDAKRLADLAERAEAL
                     GSPLVTEHIAFVRAGGPLTASPTLEAGHLLPVPRTKDALRVLCENVRIAQDSLPVPLAV
                     ENIAALINWPGEEMTEGQFLAELVERTGVRLLIDVANLHTNHVNRGEDPATALDQLPVE
                     AIAYVHVAGGVERDGVWHDTHAHPVSTPVLDVLAELRSRVSPPGVLLERDDNFPPTAEL
                     AAELDTIRATLAGPGPERTSTTLSGRAVEATETSEASNVPRAPEASPADAPTDPARERL
                     ALAQTALLSALVAGTPAPEGFDSRRLAVQSRALAGKRAEVVAKVAPDIPRILGAEYHAA
                     FLNYAKSRPMRAGYRRDALDFAEHLLIADRPEDATARRELTRWWRDRSGARPPRRATRM
                     VRAARSVLVGR"
     gene            complement(6547..7335)
                     /locus_tag="SSPS47_RS27785"
                     /old_locus_tag="SSPS47_28150"
     CDS             complement(6547..7335)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018533917.1"
                     /locus_tag="SSPS47_RS27785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_28150"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_203557937.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATVGALAFPVWSYADRSGTGEANLAASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSQAIKDAGDHLVVGHTDLDVRAR
                     DVAAKLGVALPNQPNEQQQGWLRELSAATGAEYDRKFANLLRNAHGKVFALIAEVRHTT
                     RNTLIRQLATDANQTVLDHITMLEATGLIDFDEIAREAAGTKTASPTGPPPPNGSLPPS
                     PPAASPTGDSSFTSRPSTQPGAPTAVNTNRPAPPSVEASP"
     gene            complement(7453..8268)
                     /locus_tag="SSPS47_RS27790"
                     /old_locus_tag="SSPS47_28155"
     CDS             complement(7453..8268)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523639.1"
                     /locus_tag="SSPS47_RS27790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_28155"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_239065343.1"
                     /transl_table=11
                     /translation="MDDTAAGAAGPADDRAGDPADSPFRHEQTARDEAPQFVLPLVVRI
                     EKAAPPTRTDALEAAARAVLVILADERSTGDGEWARAMRDWQDARIRKVVRRARGAEWR
                     KASLLPGITVGGPGPDAAAAREPVAAEGPGFAPYAEVRVFPPVPLDGWPKELAKLQVSG
                     TDLDDPEDPPATADPAAPVLWLSPGIAMSAGKAMAQAGHGAQLAWWELSETDRKAWQEA
                     GFPLSVRTPDAARWRQLTRSGLPLVRDAGFTEIAPGSSTVVADHPALRR"
     gene            8346..9020
                     /locus_tag="SSPS47_RS27795"
                     /old_locus_tag="SSPS47_28160"
     CDS             8346..9020
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005310477.1"
                     /locus_tag="SSPS47_RS27795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_28160"
                     /product="AIM24 family protein"
                     /protein_id="WP_164253342.1"
                     /transl_table=11
                     /translation="MKSDLFSSENMAQQATTPGMSLQNAKSVKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLPLMAVKGQGEAWFAHEAANCFIVDIDQGDTLT
                     VNGRNVLCFDSTLSYEIKTVKGAGMTGGGLFNSLFSGYGKLAIICEGNPIVIPVSPQAP
                     VYVDTDAVVGWSAQLSTSLHRSQSVGSMIRGGSGEAVQLMLQGEGFVIVRPSEVTSQAA
                     GS"
     gene            9076..9930
                     /locus_tag="SSPS47_RS27800"
                     /old_locus_tag="SSPS47_28165"
     CDS             9076..9930
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523641.1"
                     /locus_tag="SSPS47_RS27800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_28165"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_239065081.1"
                     /transl_table=11
                     /translation="MLRGVDLSLPPYSLIRLEGTNGSGKSTLLRLLAGIDSPTEGCVTG
                     RPGRTAYVPERFPVALPFTAAGYLTHMGRIHGLRGAAAKARAAEWLERFGAGGHARTAL
                     SELSKGTSQKVAVAQALLAGPELLVLDEAWTGLDSAARTQLDLAVTERVADGGTVVFVD
                     HDPRRLAGAADAVYTVAGNALARGTAPGSPAEQGPRVRVMVVGPPGAAPPDGLPGAPTH
                     ERGPEDGTVLLSVAVAHSDALLRALLAARPPWHIREVRAPAEAETEAMSTARTTSTTGH
                     ATS"
     misc_feature    complement(9115..9128)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.84"
                     /tool="antismash"
     gene            9927..10570
                     /locus_tag="SSPS47_RS27805"
                     /pseudo=""
     CDS             9927..10570
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100699.1"
                     /locus_tag="SSPS47_RS27805"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ABC transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTALFRYHTAMLLRSQRWLPPVLLCAIFLAVGVRSGEPVLDSLGV
                     AAAALLPVAAWLVRICVTQEPDAARVVTAAASGCSPRRPVRARSVSSARRSSRPSATRG
                     APTTW"
     gene            10599..10751
                     /locus_tag="SSPS47_RS34930"
                     /old_locus_tag="SSPS47_28180"
     CDS             10599..10751
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523643.1"
                     /locus_tag="SSPS47_RS34930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_28180"
                     /product="hypothetical protein"
                     /protein_id="WP_187280138.1"
                     /transl_table=11
                     /translation="MGDDANRAEIVVYADCVHCGEPSEYPESVKGITLCPVCEWHEAQR
                     TACSG"
ORIGIN
        1 gcgctgacgg tgcgaccggt gcgaccggtc cgacgggtcc ggccggtccc gtcagcacca
       61 ccgaagcggt cggcaatacg gtggcgattc cgctcgtagg aagcgttact tcagtcgcca
      121 catgccccgc tggttcggtt ctcaccggag gcggtttccg ccttaccggt cctgcggtcg
      181 tcagtttcac tgagaacgcg cgggccgccg ccccggcgga atcatggagc gtaacagtcg
      241 agggcgcgag tatcctcggt gtcagtgtca cggcggttgc tgagtgcgcg tgacggtgat
      301 tccgatctag ccgttcggca atcccttgga tatcgaggag gcgcatgtcg cgcctcctcg
      361 acatctcaaa tgcttcgatg atgtcagccc ccgaatgcgg ttggccgcgt gtacctgccg
      421 tgcggtgaat ccgaccccgg ccattctcgt tgtagtcgaa ttgagtgagg aatgcaaagg
      481 tgtccacaat ctgcttgtgc atgatcgtca aaaatgagtc gcgagtgatc gggcgttgcc
      541 tcgactccgt acgcggtctt atcgactcgt ggatcatctc ggacaccggt tcgaccgatg
      601 ggacgcagtc gctcatccga tccgcgctgg agggcatccc gggggagctc caccaggatg
      661 attgggtgaa ctttgggcac aatcgaacgc acaatattca gcacgccttc ggcagggcgg
      721 attatctgct gctgctggac gcggatcaca ccgtccgact ggagagccct cttcccgcgt
      781 tgaccggcac ttcctacatg ctgcgacatg aagggaatct ggagtatcgg gtgaagcggc
      841 tggtccgcgg cgacatcgac tggaactatg tgggcgcaac ccacgagtac ctgaacactg
      901 aagaagaaga caagcaggaa ctgctcgatg agattgtgat ctcggatttc gccgacggtg
      961 gctcgcggca tgacaagttc gagagagatg ccgcattgct ggcagacgaa ctggctcgca
     1021 atccgaagaa tgatcgtgct gtcttctatc tggcgcagac gatgcgcgac atggggaacg
     1081 gcgcagaagc cattgagctc tacgagcggc gagcaatgat gggtggatgg ggagaagaag
     1141 tctatgttgc actcctcgag gcggggatct tgcgcgctga acttggggat tggccggggg
     1201 gaatggagtg tctgaccaag gcatgggagt ctcgaccgca gcggcttgaa gcctgctatc
     1261 aactctcctc gcgcttgcgg aagatgaggc ggcataatgc tgcttacatc ttcgccaagg
     1321 ccggcatcgg tcgtccggtc cccgcggggg atgtactgtt cacgaacccc tgggtctatc
     1381 gatgggggct tctgtttgaa ctgtccgtta gctcctactg gatcggcgaa tatgccgagt
     1441 cggtctgggc ctgcgatcaa cttctaggtt tggccgacct gccggaggtg tatcggaggc
     1501 aaactgtgat caaccgcgga ttctcaatcg gtcgcctcgg tgctcgacag tgagaagtgg
     1561 gcggcccggt tccgtggcgg acttttcggg cattccctcg cttgcgtcgc cagggcacgt
     1621 gaggctggga tcgtcgcgca ccgcggaggt ttccaggagt ggacgagatc gattgcggta
     1681 ggcggaattc gcagggttcg ccgtcgcgat gagctgcggt ctcccttgat cgtgggcacc
     1741 tggggagccg ccctgcgggg ggggcttcaa gccgccacat gctgcggaag gtgacgcgca
     1801 gggccttccc cctcctgtgg aaggtgtgga gatttccaag ccgcatggca agggagtccg
     1861 tctcctcggc gtgccgaccg tgtccaaggg gatcgcccga acggcggtgg ccgctcacat
     1921 ggagaagcgg gtggatcccg tgttccaccc tggctcctac ggccaccggc ccggaaggtc
     1981 ggccctcgac gcgatggccc ttgcgtgaac ccgcgaaaac gcgatttacc cggccttggg
     2041 gaaactgcac aggcgccatt gcgcctggac actgatcacg tcccggccgg agttcgcctc
     2101 tcatgcgtgt acgccgtggg acgagatgat gagccgtgct ccaatgatgc ttccacggac
     2161 tcggaccgaa cgcctgagtt cagccttctt cgcagttcag tgtgctctgc cagcgacacc
     2221 gggttcggtg ccgcgtgcgg cgcgcactct gcgcgccgca ccggtgtctt cccggtcagc
     2281 agatacgcct cgacgtggtc gttgacgcac tcgttggtgc cgcccgcgat gccgtgcgtg
     2341 cccgcgccgc gctcggtcac caggacggat ccggccaggc gccgctggag ctccagtgcc
     2401 cccgcgtacg gcgtcgccgc gtcccgctcg gcggccagga tcagcgtcgg tggcagctca
     2461 ccggcgctcg tgcggacgtc caccggccgc cggcgctggg ccggccagaa cgcgcacggc
     2521 agattcatcc acgcgttgtc ccacgtctcg aacggcgcga cctccgcgag accggtgttg
     2581 tcgcggtccc acaccttcca gtccgtcggc cacggcgcgt cgttgcactc cacggccgtg
     2641 tagacggcgt tgctgttctc gttgcccacg gccgccgccg gccgcggcgc ggcctggtcg
     2701 acgaggggct tcgtgttgcc cttcaggaac tcggacaggg ccgtggcccg aatcgcccag
     2761 tagtcgtcgt agtagcccgc cccgagcatc gccgcgtgga gctggccggg gccgaccttg
     2821 ccgcccgccg gccggtgcgc gacctccgcc cgtacccgct cgtagttgcg ctggacctcg
     2881 gcgggcgtgg tgcccaggtg gtacgtcgcg tcgtgcttgg cgacccaggc gcggaagtcc
     2941 gcccagcgcc gctcgaaggc gaaggactgg tccatgttgt tgcggtacca gatctgctcc
     3001 gggtccggat tgacggccga gtcgaagacc atccggcgta cgtgggaggg gaagagcgac
     3061 gcgtacagcg cgccgaagta cgttccgtag gaggcgccca tgaaggacag cttcttctca
     3121 ccgagtccgg cccgcaggac gtcgaggtcg cgggcgttgt tcagcgacgt gtagtggcgc
     3181 agggacgctc ccgcgttgcg cgcgcagccc tgggcgtacg ccttggcgcg ggagatccgc
     3241 tccctcttgt acgactccgt gggatacgtc ggcgagagcg tgggggcctt cgcgaagtcc
     3301 ccggcgttct ggcaggagag cggcgcggag cggccgaccc cgcgcggggc gtagccgacc
     3361 aggtcgtacg ccgccgcgat ccgcttccac tcgggcatct tggccgccaa cgggaaggcc
     3421 atgccggagg agccggggcc gccggggttg tagaccagcg cgccctggcg ccggacggtc
     3481 tccacggcct tgccgtcgcc gcccgcggcg cccgtcctgc ccgacgcctt cacccggctg
     3541 acggtgagtt tgatccgctc accgtccggc cgggcgtagt cgagcggcac ctcgaccgtg
     3601 ccgcaggtga ggggggcggg cagcatctcg gccttggggc aggcccggaa ggtgatcccc
     3661 gcggcggcgg cgcgcttggc ggcgacgacg gtgccgcgcg cctcggacga ggtgccggcg
     3721 aagtccgcgc cgcccgccgg tgcggcggag agggtggaca ggaccaagga tccgagggtg
     3781 gccagggccc cgtacttcgc aactgctctc atcgctttcc cttcaggcgt gccgcgttgg
     3841 ggggaaggcg atcatctccg gagccccgga gatgtaaagg atctgtgagc ggtgtcgggg
     3901 cccatgaccc cgatgcgtca tacagtcact caatgggccc gtacggggtg cgtgatcggg
     3961 tgcgctgtgc tccgtgcgcg gggtgcgagc gcgagtgccg accgccccgt acggccgtac
     4021 ggggcgggcg ccggtcagcc tccgccgcca ccacctccgc agcctccgcc tccgccacag
     4081 ccgccaccac cgcctcctcc tcctccgtca ccggagccgc cgcccccacc gcaccctccg
     4141 cccgaaccgc cgcagcccga accgccggag ccgccgcagc cggacccgcc cgaaccgccg
     4201 tccccgaagt ccgcactggc gcaccacacc accgcggtgg cggccagatg cggcgcgggc
     4261 tggaagtcac ggccgggccg catgccccgc gcggcggtca gctgctcccg cagcagcggg
     4321 tcgtccagcg ccatgtgacc gtccacggcg atctggtggt cgggcgtcgt gatgtgccgg
     4381 tacgccgtac ggaacttcag ccgagcccgc acaccggccg cggtgagccg ccgcctggcc
     4441 agcgcggcac aggcgaagcc cgagatgatc ccgatcattg ccagcggcag cgtcgggatg
     4501 accgcgggcg aggcggtgct gatgagccgg acgatggtca gcggcacggc gagcaggcac
     4561 acgatcgcct gtgccagccc ccagaccgcc cacacgcggg tcttcctggc cggcaccagc
     4621 aggccgcgcg ccgccaggga gtccccgatg tcctggacgg cacggccgcg catcactgcg
     4681 agacgcagcg cgtgcagggc gccgtgcggc gcccggttgt gctcatcgat gacggcacgt
     4741 tccaccgggt tgcgggcgac gtcgctgtac agggcgacga tgccggggcc gccgatgccc
     4801 agcctgccgt ccgcgtgcat cgcgacgacg gcggtctcgg cgaccctgcc cggtccgccg
     4861 ccgaggaagg ccacgtccat gaggtcgtgg acctcggtcg taccgccgct cgaccgcgcg
     4921 gtgatcactc cgacgatgag cgtgacgaag gacaggggta tggcgatctg gagtgtggtg
     4981 atcagtgtgt ccacggccgg tcaccgcccc acgagtacgg agcgggcggc acgtaccata
     5041 cgggtcgcgc ggcgcggcgg tcgcgcgccg gagcggtccc gccaccaccg ggtcagctcg
     5101 cggcgggccg tcgcgtcctc gggccggtcg gcgatgagca ggtgctcggc gaagtcgagc
     5161 gcgtcgcgcc ggtaaccggc gcgcatgggg cgggacttgg catagttgag gaacgcggcg
     5221 tggtattcgg cgccgaggat gcgggggatg tcgggcgcca ccttggcgac gacctccgcc
     5281 cgcttgcccg cgagggcccg gctctgcacc gcgagccgcc gtgagtcgaa gccctccggg
     5341 gcgggggtcc cggcgaccag ggcggacagc agtgcggtct gtgcgagggc gagccgctcg
     5401 cgggcggggt cggtcggcgc gtcggccgga gaggcttcgg gcgccctcgg cacgttggat
     5461 gcctcggatg tctcggttgc ctcaacggcc ctgccggaca gcgtggttga cgtccgctcg
     5521 ggcccggggc cggccagcgt cgcacggatc gtgtcgagtt cggcggcgag ttcggcggtc
     5581 ggcgggaagt tgtcgtcccg ctccagcagg acgccgggcg gtgagacccg cgagcgcagc
     5641 tccgcgagta cgtcgagcac cggcgtgctc accgggtggg cgtgcgtgtc gtgccagacg
     5701 ccgtcgcgtt cgacgccgcc cgcgacatgg acgtacgcga tggcctcgac cggcagttgg
     5761 tccagtgcgg tcgccgggtc ctccccccgg ttgacgtggt tggtgtgcag gttcgcgacg
     5821 tcgatcagca gccgtacgcc cgtacgctcg accagctcgg ccaggaactg accctccgtc
     5881 atctcctcgc ccggccagtt gatcagcgcg gcgatgttct ccacggcgag cggcacgggc
     5941 agggagtcct gcgcgatgcg cacgttctcg cacagcaccc gcagggcgtc cttggtgcgg
     6001 gggacgggca gcagatggcc ggcttcgagt gtgggggacg cggtgagcgg gccaccggcc
     6061 cggacgaagg cgatgtgctc ggtgaccagc ggggagccga gggcctcggc ccgctcggcg
     6121 agatcggcga gacgcttggc gtcgggccgc gcggcgcctc ccaggccgag ggagacgccg
     6181 tgcgggatca cggtgacgcc gcgtgcgcgg agccggacga gcgagtcggg gagatggccc
     6241 gcgcagacgt tctcggcgac ggcctcgacc cagtcgatgc cgggcagcgc ctccaccgcc
     6301 tccgcgatct cgggccgcca gccgataccg atacccaggg ccctcaggcc tcccggatac
     6361 ctcgcgttcc cgacttctcc cacgttcacc acgtgtgtcg tgcggctcat gtctccccct
     6421 ttttccgtgc ctcggcgtgc gccggttcag gggtaatggc cccgccgggc gccggtgaat
     6481 ccagaggagg ggacgttcag agcatcattt gaggttcccg tgacggccgg tgatccggcc
     6541 gatgactcac gggctcgcct cgacgctcgg cggcgccgga cggttggtgt tgacggccgt
     6601 cggagcgccc ggctgggtgg agggccggga ggtgaaggag ctgtcaccgg tcggtgaggc
     6661 ggcgggcggt gacggcggga gactgccgtt cggcggcggc ggaccggtgg ggctggcggt
     6721 cttggtgccc gccgcctcgc gcgcgatctc gtcgaagtcg atgagcccgg tggcctccag
     6781 catcgtgatg tggtccagga ccgtctggtt ggcgtcggtc gcgagctgcc gtatgagggt
     6841 gttgcgtgtc gtatgtctga cttctgctat cagtgcgaag accttgccgt gggcgttgcg
     6901 caggaggttg gcgaacttcc tgtcgtactc ggcgccggtg gccgccgaca gctccctgag
     6961 ccagccctgc tgctgctcgt tgggctggtt cggcagcgcg acgccgagct tcgccgccac
     7021 gtcgcgcgcc cggacgtcga ggtcggtgtg gccgacgacg agatggtcgc ccgcgtcctt
     7081 gatggcctgg ctgggggcac gctcgatcgc ctgctgcccc gcgggcagct cccacagccc
     7141 cgcgagccgt accttcacga ggaagtcgcg gtccgtcgcc gagagcggac cccactgggt
     7201 ggccacgctc gacgccgcga gattcgcctc ccccgtgccg gaacggtcgg cgtacgacca
     7261 gacaggaaag gcgagcgccc cgaccgtcgc gacgagagcc gcgatgatga gtgccgtgcc
     7321 gttgatacgt cgcaacaaag tgtctcccgg gccgatgtac aaccgcgtac cactgcggaa
     7381 tcaaccgcgc gctgaccggt cgtgcaacct acttagcggt attgggagat acgaatcctg
     7441 gcacgggaat gttcaacgtc gcagcgcggg atggtcagcg accaccgtgg acgaccccgg
     7501 cgcgatctcc gtgaacccgg cgtcacggac caacggcagc ccgctgcggg tcagttgacg
     7561 ccagcgcgcg gcgtccggag tgcgtacgga gagcgggaat ccggcctcct gccacgcctt
     7621 gcggtccgtc tccgacagct cccaccaggc gagttgcgcg ccgtggccgg cctgagccat
     7681 cgccttgccc gccgacatgg cgatgccggg gctgagccag agcacgggag cggcggggtc
     7741 ggcggtggcg ggcgggtcct cggggtcgtc caggtcggtg ccggagacct ggagcttcgc
     7801 cagctccttc ggccagccgt ccagcgggac gggcgggaag acccgtacct ccgcgtacgg
     7861 ggcgaagccg ggaccctcgg ccgccacagg ttcccgcgcc gccgcggcgt ccggccccgg
     7921 accgcccacc gtgatccccg gcagcagcga cgccttccgc cactccgcgc cgcgcgccct
     7981 gcgcaccacc ttgcggatcc gggcgtcctg ccagtcccgc atcgcccgcg cccactcgcc
     8041 gtcacccgtc gaccgctcgt cggccaggat gaccaggacg gcgcgggcgg cggcctccag
     8101 cgcgtcggta cgggtcggtg gcgcggcctt ctcgatccgt accaccagcg gcagcacgaa
     8161 ctgcggggcc tcgtcgcgcg cggtctgctc gtgccggaac gggctgtcgg ccgggtcccc
     8221 ggcgcggtcg tccgccgggc cggccgcccc ggcggcggtg tcgtcgatgc tcacggggcc
     8281 agtgtgccag caccgaccgg ccggattgtt ggcggaatgg gcgcctccgg gtgaccatgc
     8341 tcggcatgaa gagcgatctc ttttccagcg agaacatggc gcagcaggcg acgacccccg
     8401 ggatgagcct ccagaacgcc aaatccgtca agtacgccgt caacggcgaa atgcacgcgc
     8461 gtcagggatc gatgatcgcc ttccgcggca atctgcagtt cgaacgcaag ggccagggca
     8521 tcggcggcat gctcaagcgc gccgtcaccg gcgagggcct gccgctgatg gcggtgaagg
     8581 gccagggcga ggcctggttc gcgcacgagg cggccaactg tttcatcgtc gacatcgacc
     8641 agggcgacac gctcaccgtc aacggccgca acgtcctgtg tttcgactcg acactctcct
     8701 acgagatcaa gacggtgaag ggcgccggca tgaccggtgg cggtctcttc aacagcctgt
     8761 tcagcggtta cgggaagctc gccatcatct gtgagggcaa ccccatcgtc atcccggtca
     8821 gcccgcaggc gccggtgtac gtcgacaccg acgccgtcgt cggctggagc gcccagctga
     8881 gcacctcgct gcaccgctcg cagtccgtcg gctcgatgat ccgcggcggt tccggcgagg
     8941 ccgtccaact gatgctccag ggcgagggat tcgtcatcgt ccgcccgagc gaggtcacgt
     9001 cgcaggccgc cggaagctga ctcccgccat ggaactgcga ggagtgggcc gccggtacgg
     9061 tgtcggcggc cctgggtgct gcggggcgtc gacctctcgc tgccgccgta cagcctgatc
     9121 cgcctcgaag gcaccaacgg cagcggcaag tcgaccctgc tgcgcctgct cgccgggatc
     9181 gactcgccca ccgagggctg cgtcaccggc cgccccggcc gtacggcgta cgtccccgaa
     9241 cgcttcccgg tcgccctgcc gttcaccgcc gccgggtatc tcacgcacat gggccggatc
     9301 cacggactgc gcggggccgc cgcgaaggcg cgcgccgccg agtggcttga gcgcttcggg
     9361 gccgggggcc acgcccgtac ggcgctctcc gagctgtcca agggcaccag ccaaaaggtc
     9421 gccgtggcgc aggcgctgct cgccgggccc gagttgctgg tactcgacga ggcctggacg
     9481 gggctcgact ccgcggcccg tacgcaactc gatctggcgg tcaccgaacg cgtcgcggac
     9541 ggcggcacgg tggtcttcgt ggaccacgat ccacgacgcc tggcgggggc ggccgacgct
     9601 gtgtacacgg tggcgggcaa cgcgctcgca cgcggtacgg cgccgggctc cccggccgaa
     9661 caggggccgc gggtaagggt catggtcgtc ggaccgccgg gtgcggcgcc gccggacgga
     9721 ctgcccggcg cacccacgca cgagcggggg cccgaggacg ggaccgtcct gctgagtgtc
     9781 gccgtcgcgc actccgacgc cctgctgcgc gcgctgctcg cggcgcgccc gccctggcac
     9841 atccgggaag tacgtgcgcc ggccgaggcc gagacagagg cgatgagcac cgcacgcacc
     9901 acgagcacga cggggcatgc cacttcatga ccgcactgtt ccgctaccac accgccatgc
     9961 tgctgcgctc gcagcgctgg ctcccgccgg tcctgctctg tgcgatcttc ctcgccgtcg
    10021 gtgtccggag cggcgagccg gtgctcgact cgctcggtgt cgccgccgcc gcgctgctgc
    10081 cggtcgccgc gtggctggtg cggatctgcg tcacgcagga accggacgcg gcccgcgtcg
    10141 tcacggcagc cgcgtcgggc tgctcgccgc gacggcctgt gcgggcgcgc tcggtgtcgt
    10201 cggcacggcg gtcgtcacgg ccatcagcga cccgaggagc gccgaccacc tggtgaccgt
    10261 ggctccgctg ccggccggga cggcggggct gctcgcggcc caggtctgtc tgctgaccgg
    10321 tgccacggtc ggcgcgctgt tctcccggcc gctgctgcgc ggccgtggct ggtcgctcgc
    10381 ggcgatctcg ctcttcgcgc tgctcgcgct ggtgaccacc gggtctccgg tgaaggccgc
    10441 ggtgaccggc ctggtgaccg gttcgcgtac gggcgtgatc cacacgccgg gacggtcgct
    10501 ggccgccgcc gcgctggtgg cggccgtggc ggcggcgctg gtctgtgtcc tcggggtacg
    10561 gcgcggctga gggcgcggcg ttcagtacgc tcggcggtat gggagacgac gccaaccgtg
    10621 ccgagatcgt cgtgtacgcg gactgtgtgc actgcggaga gccgagcgag tacccggaat
    10681 cggtgaaggg catcacgctg tgccccgtct gcgaatggca cgaggcgcag cggacggcct
    10741 gttccggctg aggatccgct ccggccggcg gtcggccggg tcagcccttg atcagctccg
    10801 acaccttgac gaagcggtag ccgcgctcgc gcagtacggg cacgatcttg cgcaccgcct
    10861 cgtccgtcac cggcgccgcg ctcatggtgc agtgcatgac caccaccgag ccgggccgta
    10921 cgtcggcgag cacctgctcg gccaccgtgt cggcgtccgt ggcgaaggcg tcgccgctga
    10981 cgacgtccca ctggaccgcc gtcagaccgg ccggtgccag ggtccgcaga gtcgaccggt
    11041 cgtaacagcc gccggggaag cggaagtacg gcaccacctg gcggaccccg gccttgcgga
    11101 aggcgccgaa ggcccgctcc acatcggccg ccatgtcctt ctcgccgagt gccttgagcc
    11161 cgtggcagtc ggccgtgaag gagtggtggc tgtacgagtg gttcgcgacc tcgaacaggg
    11221 ggtcggagcc gatctccctc gcctggaagg ggtactcctc cacccaccgt cccgtcatga
    11281 agatcgtcga ggggacttcg aggcggcgca gcgaggagat cagggtcggg ttgtcgaagc
    11341 gctcgccacg ggcggcgcgc ggtccctggt cggcggtcat gtcggcgtcg aaggtgagcg
    11401 cgacggtctt
//
