LOCUS       NZ_CP048397            10903 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. S4.7 chromosome, complete genome.
ACCESSION   NZ_CP048397
VERSION     NZ_CP048397.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. S4.7
  ORGANISM  Streptomyces sp. S4.7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7920066)
  AUTHORS   Bekiesch,P., Zehl,M., Domingo-Contreras,E., Martin,J.,
            Perez-Victoria,I., Reyes,F., Kaplan,A., Ruckert,C., Busche,T.,
            Kalinowski,J. and Zotchev,S.
  TITLE     Viennamycins: new lipopeptides produced by Streptomyces sp. S4.7
            isolated from the rhizosphere of Edelweiss (Leontopodium nivale
            subsp. alpinum)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7920066)
  AUTHORS   Bekiesch,P., Zehl,M., Domingo-Contreras,E., Martin,J.,
            Perez-Victoria,I., Reyes,F., Kaplan,A., Ruckert,C., Busche,T.,
            Kalinowski,J. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2020) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.8x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010384365.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 10:27:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,027
            CDSs (total)                      :: 6,939
            Genes (coding)                    :: 6,650
            CDSs (with protein)               :: 6,650
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 103 of 289
            Pseudo Genes (incomplete)         :: 223 of 289
            Pseudo Genes (internal stop)      :: 23 of 289
            Pseudo Genes (multiple problems)  :: 52 of 289
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5895235
            Orig. end                         :: 5906138
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048397.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zotchev Lab, University of
            Vienna, Austria.
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10903
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="16"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10903
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10903
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5903](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5903
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            27..221
                     /locus_tag="SSPS47_RS26250"
                     /old_locus_tag="SSPS47_26555"
     CDS             27..221
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470322.1"
                     /locus_tag="SSPS47_RS26250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26555"
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_031089472.1"
                     /transl_table=11
                     /translation="MAMTLRLPDDLDAKLTERARRERRSKQELAIEAIRDAQDRAELKV
                     DDVLAELMDSDAEILDYLK"
     gene            218..610
                     /locus_tag="SSPS47_RS26255"
                     /old_locus_tag="SSPS47_26560"
     CDS             218..610
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504909.1"
                     /locus_tag="SSPS47_RS26255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26560"
                     /product="Fic family protein"
                     /protein_id="WP_164253112.1"
                     /transl_table=11
                     /translation="MTEVRYIQIDEILAIARAVNGTEHSVRDMGLLVSAIERPRTNVFG
                     AELYPTLHEKAAASLHSVARNHALIDGNKRTAWLAMRAFLRLNGLSASTVPPHVSVAGP
                     FVEEVAQDNIDVPAIAKRLSAWFPVS"
     gene            <817..1071
                     /locus_tag="SSPS47_RS35590"
                     /pseudo=""
     CDS             <817..1071
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153560.1"
                     /locus_tag="SSPS47_RS35590"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="VPGADAIDAPCANAATGDT"
     gene            complement(1111..1548)
                     /locus_tag="SSPS47_RS26260"
                     /old_locus_tag="SSPS47_26565"
     CDS             complement(1111..1548)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445181.1"
                     /locus_tag="SSPS47_RS26260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26565"
                     /product="hypothetical protein"
                     /protein_id="WP_164253113.1"
                     /transl_table=11
                     /translation="MDSELAVLVASGATTLVSLMVTDSWTHARELVGRYLTRTGADPGT
                     FDDLQDARALLLAADATERERTAREISRRYGARLRRLAEADGSDEALRALLTSLLQLSA
                     SATDSWRMVDNRISGGVQHGPVIQSGTVAGLTFHVDHVDRQ"
     misc_feature    complement(1252..1254)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(1559..1762)
                     /locus_tag="SSPS47_RS26265"
                     /old_locus_tag="SSPS47_26570"
     CDS             complement(1559..1762)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SSPS47_RS26265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SSPS47_26570"
                     /product="hypothetical protein"
                     /protein_id="WP_164253114.1"
                     /transl_table=11
                     /translation="MHLSSFLNSIGFTDGDVRRPMLAAQIAGLIKEQNRHETADEIQHH
                     FDPPASLAPDDDEAPASGSPLA"
     misc_feature    1915..1930
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.66"
                     /tool="antismash"
     gene            2934..3341
                     /locus_tag="SSPS47_RS26270"
                     /old_locus_tag="SSPS47_26575"
     CDS             2934..3341
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980515.1"
                     /locus_tag="SSPS47_RS26270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26575"
                     /product="SHOCT domain-containing protein"
                     /protein_id="WP_164253115.1"
                     /transl_table=11
                     /translation="MTGEMNLANDYPILGAFWTVLFFVLSVLWLILLFRIIADILRDDM
                     AGGVKAGWLLFVILLPFLGVFVYVAVRGRGMGEREQRQTRARQQDFDSYIRATAGAGTS
                     QAEQLAKLVEMHDRGAISDHEFQKAKTKVLH"
     misc_feature    2952..2970
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.74"
                     /tool="antismash"
     gene            complement(3358..4041)
                     /locus_tag="SSPS47_RS26275"
                     /old_locus_tag="SSPS47_26580"
     CDS             complement(3358..4041)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557670.1"
                     /locus_tag="SSPS47_RS26275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26580"
                     /product="pyridoxamine 5'-phosphate oxidase family protein"
                     /protein_id="WP_239065049.1"
                     /transl_table=11
                     /translation="MEKRTSSDGAAGGGPGAPENVADRVELRRIQLGLSPDELAHRAQM
                     SLSYLRELASLHGDFDPEGLTRLANALDMTSVELLHGRADPPPGQPGAAAHPRLRRLTE
                     HECWDRLDTHGVGRLGLRGDTAPVVVPVNYLVDGLSVVYRSEPGSVPAVDAGTSLSFEA
                     DHLDEARMSGWSVLLTGTAEHITDPGATGALADRAGSRPWAGGSRPLWIRLLPEQISGR
                     VIDTH"
     gene            complement(4185..4877)
                     /locus_tag="SSPS47_RS26280"
                     /old_locus_tag="SSPS47_26585"
     CDS             complement(4185..4877)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543193.1"
                     /locus_tag="SSPS47_RS26280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26585"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_203558108.1"
                     /transl_table=11
                     /translation="MQGRAKATRRSLLEAAAQLFEERGYAGTSISDISALSGRTSGAIY
                     FHYACKENLALAVMEEVFDTWPTLVRRHREADIPALEKLVALSFSVARAFRDDVVVRGG
                     SRLWAERKSVDTSLPSPFEGWIETVVGLLEEAGREGVLTPSVIPRKAGNAVVSAFFGLH
                     TVSDALDGREHIEERLHDLWLLLLPALQARPGATSLLDRAYAHLTEASGTPAPAGPQVR
                     KGTGTARR"
     misc_feature    complement(4982..4996)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 20.24"
                     /tool="antismash"
     gene            5001..5903
                     /locus_tag="SSPS47_RS26285"
                     /old_locus_tag="SSPS47_26590"
     CDS             5001..5903
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543192.1"
                     /locus_tag="SSPS47_RS26285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26590"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_239065050.1"
                     /sec_met_domain="AfsA (E-value: 1.4e-15, bitscore: 55.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGVFTGESRLSWSRTVPRELVHRRSAAEVLLTDVRPGRQRGVFDA
                     AASWSRSHPTFPRDGADLHSPLIVIETMRQLGIYLPLRHYAVPPESRLLITDLFFRIDP
                     EREPRATSNSTDVTCRARVTDIRNGADGHVAGLRMEISVRTGSGIFARAGGGARFLGPE
                     QYAAVRGAGATALPPAPTGPRDRPDIGRIAVADPRDVMIALDDGAVRLRPADPRHPFFF
                     DHPSDHVPGMVLLEAARQAGALASEGRCVRPTAGRLKALSFAEFAPAARVLAVPHHRTC
                     GFRVLQGGRYVAYGALEYR"
     gene            6135..6827
                     /locus_tag="SSPS47_RS26290"
                     /old_locus_tag="SSPS47_26595"
     CDS             6135..6827
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006601759.1"
                     /locus_tag="SSPS47_RS26290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26595"
                     /product="HAD family phosphatase"
                     /protein_id="WP_164253118.1"
                     /sec_met_domain="HAD_2 (E-value: 7.2e-25, bitscore: 86.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSERVLSWTPSAIVFDCDGTLMDTERHWQNAREHALGEFGLAPA
                     PGFADRAKGLHYTQCGLLMAEEAGRPDLAADMTGSLLRIFRDLVAENPLTMPGARELVV
                     SAATFAPLAVASNCPREVVESCLDTAGLRDYFGHIVVPDTGIRPKPQPDVYLAAARHCG
                     TDPADTLAVEDSLCGVEAASRAGMRVLGVGPWPGERTTAMVDLWVSSLAEPDLSRWAAA
                     RIPRQATR"
     misc_feature    6160..6174
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     gene            6918..7811
                     /locus_tag="SSPS47_RS26295"
                     /old_locus_tag="SSPS47_26600"
     CDS             6918..7811
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037746.1"
                     /locus_tag="SSPS47_RS26295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26600"
                     /product="oxygenase MpaB family protein"
                     /protein_id="WP_164253119.1"
                     /transl_table=11
                     /translation="MSPFGKSAPDLRGRVGGALFARVAGPAGQENRARIHDTPGPRWFE
                     ADRPVRTVHGDASMFIGGLRALLLQSLHPLAMAAVAAHSGYRGDPWGRLQRTSTFLAMT
                     TYGTADDAQAAVDRVRAVHEGIRGRTASGEAYHAADPHLLGWVHAAEVDSFLMAHQRYG
                     ADPLDGAGLDGYVADTAHVAEALGVVDPPRTREELTAVLTAYRPELRPTREALEAAHFI
                     LHHPPLPLVARPPYAVLAANAIVTLPPWARTALRVRPPPLLPEGCVEPAGRLLTRTIRW
                     AMPPAPPLTTSGANAG"
     gene            complement(7956..8381)
                     /locus_tag="SSPS47_RS26300"
                     /old_locus_tag="SSPS47_26605"
     CDS             complement(7956..8381)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019359861.1"
                     /locus_tag="SSPS47_RS26300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26605"
                     /product="hypothetical protein"
                     /protein_id="WP_164253120.1"
                     /transl_table=11
                     /translation="MSETNLPSAELRALDRLVGTWAVTGGAEGTVRYEWMPGRFFLIQH
                     VELTQSGEPVTGMELIGNLRPFGEPVGTDVVSRYYDSNGNTFDYVYELAGDKLTIWAGA
                     KGSPAYYEGTFSADGTTATGDWVYPGGGGYTSTMTRI"
     misc_feature    8366..8380
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.9"
                     /tool="antismash"
     misc_feature    complement(8449..8464)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            8532..9212
                     /locus_tag="SSPS47_RS26305"
                     /old_locus_tag="SSPS47_26610"
     CDS             8532..9212
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019359862.1"
                     /locus_tag="SSPS47_RS26305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26610"
                     /product="DUF998 domain-containing protein"
                     /protein_id="WP_164253121.1"
                     /transl_table=11
                     /translation="MTVPAVPAAPAVSTTGVVSTARSTARSSGRLLAAGALAGPVFAAS
                     AVIQMFTRTGFDIARHPISQLSTGGLGWIQITTFVLAGLGALALAAGIGRTLREGYGRR
                     VLPVCVGIFGVGLIAAGLFPMDPENGFPVGTPDRPVAEMSWHGVAHSASAAIAFTALAV
                     AAIVLTVRCVRRHAVLPAVLNGAAALVLVLPMAPDHMSIQIAVNGLVAFTWTTVVAMSL
                     RRTR"
     gene            9256..9627
                     /locus_tag="SSPS47_RS26310"
                     /old_locus_tag="SSPS47_26615"
     CDS             9256..9627
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405085.1"
                     /locus_tag="SSPS47_RS26310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_26615"
                     /product="YciI family protein"
                     /protein_id="WP_164253122.1"
                     /transl_table=11
                     /translation="MKYLLLGYTSAAAWDAATADSPSPEALAAFAVYQRFERELIDTGE
                     LVTTEGLGHPAVSTTVHRTPDGMMATDGPFAELKEVLASFAVIDVADRERAVEIASRIV
                     EVLGEPIEIRPIMSEDFTA"
ORIGIN
        1 agataccagt ccggtatcat cgctgtatgg cgatgacact ccgacttccc gacgaccttg
       61 acgcgaagct caccgagcgg gctcgtcggg agcgccgcag caagcaggag cttgccattg
      121 aggccatccg tgacgcgcag gatcgggccg agctgaaggt cgatgacgtc ctggcggagc
      181 tcatggacag cgatgcggag atcctggact atctgaagtg accgaagtgc gctatatcca
      241 gatcgacgag attctggcca tcgctcgcgc ggtcaatggt accgagcaca gcgtgcggga
      301 catgggactc ttggtgtccg cgatcgaacg gccccggacg aacgtgttcg gggccgagct
      361 gtatcccacg ctgcacgaga aagcggcggc atcgctgcac tctgttgccc gcaatcacgc
      421 gctgatcgac ggcaacaaac gcaccgcctg gctcgcgatg cgcgccttcc tgcggctgaa
      481 cggcctcagc gccagtaccg tcccgccaca cgtctccgtt gccggcccgt tcgtcgagga
      541 agtcgcacag gacaacatcg atgtaccggc cattgccaag cgcctctcgg cctggttccc
      601 cgtttcctga cgttcgacga cgtgagggca gggcacgcgc gtgccctgcc ctcacacgcg
      661 gtccggccct gcccaggtca cgccggaatg ggacgctgtg tgctggaagg catggctccc
      721 tttggacgcg gcataccgga cagcggccgc aggctttccg ctcgagttct tggctcgggt
      781 caccgggtga cgctgggttc cttaccccgg cgtcacgtac ccggcgccga tgcgatcgat
      841 gccccctgcg ccaacgcggc gacgggggac acctgagtcg acgcacacct cttgccggtc
      901 cccgccccgt cgctcgacgc gatgtcgacg atgacggcga cctcgccgaa tggattccag
      961 ggtgccgtga ccatcgggcg cgcggagcta tgcggcgcgg ctgccctcgg gcgcccgact
     1021 cgcattgccc acgacacccg tatcatccgc gccggggtgg gaacgggttg acagagcaca
     1081 ggagtgcgcg gtgctctccc cgcactggcg ctactggcga tcgacgtgat ccacgtggaa
     1141 ggtcagcccg gcaacggtgc ccgactggat gacaggaccg tgctggactc cgccgctgat
     1201 gcggttgtcc accatgcgcc agctgtctgt cgcggacgcc gagagttgca gtaacgaggt
     1261 cagcagagcg cgcagggctt cgtcgctgcc gtcggcttcc gcgagtcggc gtagacgggc
     1321 accgtatcgt cggctgatct cgcgggcggt ccgttcacgt tccgtggcat cggcggccag
     1381 gagcagggcc ctcgcgtcct gcaggtcgtc gaacgtgccg gggtccgcgc ctgtgcgggt
     1441 caggtaccgc ccgacgagtt cccgggcgtg tgtccaggag tcggtgacca tgagagagac
     1501 cagcgtggtg gcgcccgatg ccacgagtac ggccaattcg ctgtccatgc ttcttctcct
     1561 aggcgagggg ggagccggag gcaggtgcct cgtcgtcgtc cggggcgagg gacgccggcg
     1621 gatcgaagtg gtgctggatc tcgtcggcgg tctcgtggcg gttctgctct ttgatgaggc
     1681 cggcgatctg tgcggcgagc atcgggcggc gtacatctcc gtcggtgaaa ccgatgctgt
     1741 tcaggaagct ggacagatgc atcgtggccg tctcccgtcc ctgtggcgct gagggaggct
     1801 cctccctggg aaagtccgac gtcgtcggct cggcggcctg ctgagagatc agatcctgaa
     1861 gccgttcggg aatgtcggtg tcattggccg ccgaagtgag ctgagcgagc agggctaggt
     1921 ccagaccacg gcgctgccgg tgtttttgag gacgtcctcg accaagtacc ggcgcctgct
     1981 ctgctcgtac ttgcgctggt gcacccagac ttcctccgac ctgcctggtt ggcgcactgg
     2041 ttggagtcgg tgcgtttgcc ggcacggcca tcgacgagag gatgtggctg gagttgatgc
     2101 acctcggcgc cgcggattct ccggccttta cggacgctga cttcgtcacc gaacacaccc
     2161 ccgtccgccc cagccacggg gacgtcctgc cgctcgtcaa ccgtgacgct cgtttgacgc
     2221 tccaggagct cacacgcagg acggcgagtt gaagcaaccg ctctgacctg gggttgtcca
     2281 tgacgtgcgc aggctgacat ccttccgatg gcgcatcatg tggagtgtgt cgcgatcctg
     2341 gagccggtga agaaggacga ctgacctgac ggtcggcgcc gtcctggtcc cggtcgcggt
     2401 gagggctggg gccgaaggtg tgcgggcggg gcagggactg ctcatcgagg ccatcgcgtg
     2461 gagcagctcg ttgagcgcca cccggtcgcc ttgctggccc gtggccgcat catgggcttg
     2521 aggcggcctg cgaggggcgg cagaactcgc cctgcgtccc ggaccacttg acggacgcag
     2581 ttgtcggggt gggcggcgga ggcagggtcg tcgaccggga ggtactaact agccgccgct
     2641 ctcgccctcg atcgtccggc gcggcgagcg ggggcggggt gtcgggtcga gggtgggcag
     2701 gggcggggag gccagaccgc ccaggggccg agcagacgtt gcaggcggtc cccgcggcgt
     2761 tgcgtggcgc cgggcgagcc gtagccgggt ggcaggtggt ggctcgaagc cgcaccatga
     2821 cggtgaggtt cccgcatccg cggcatcgtg cggccgccga ctccggaaga cgcgtagcgc
     2881 gggaccggag ggcgaggacg tatccaccac cccctacgag cggagaagga accatgaccg
     2941 gagagatgaa cctggcgaac gactatccga tcctgggcgc gttctggacg gtcctcttct
     3001 tcgtcctgag cgtgctgtgg ctgattctgc ttttccgaat catcgcggac atactcaggg
     3061 acgacatggc cggaggagtc aaggccggct ggctgctgtt cgtgatcctc ctccccttcc
     3121 tcggggtctt cgtctatgtc gccgtccgag gccgcggcat gggagaacgt gaacagcgcc
     3181 aaacgcgggc ccggcagcag gatttcgact cctacatccg cgcgaccgcc ggagccggca
     3241 ccagtcaggc ggaacaactg gcgaagctgg tcgagatgca cgacaggggt gccatcagcg
     3301 atcacgaatt ccagaaggcg aagaccaagg tgctccactg atgccctgag agccacatca
     3361 gtgtgtgtcg atcacccggc cgctgatctg ctccggcagc agccggatcc acaggggccg
     3421 cgacccgccc gcccagggac gtgagcccgc ccggtccgcg agtgcgcccg ttgccccggg
     3481 atccgtgatg tgctcggccg tccctgtcag cagcacgctc cagccgctca tccgcgcttc
     3541 gtcgaggtgg tcggcctcga aggagagtga cgtgcccgcg tcgacggcgg ggacgctgcc
     3601 gggctcgctc cggtacacga cgctcagccc gtccacgaga tagttgaccg gtacgaccac
     3661 gggcgcggtg tctccgcgta gccccaggcg cccgacccca tgggtgtcca gccggtccca
     3721 gcactcgtgc tccgtgagcc gcctcagacg cggatgggcc gcggcgcctg gctggcccgg
     3781 cggcgggtcg gcgcgtccgt ggagcagttc gacggatgtc atgtccaggg cgttggccag
     3841 tcgggtcagc ccctcgggat cgaagtcgcc gtgcagcgaa gccagctccc gcaggtagga
     3901 cagggacatc tgggcgcggt gcgccaactc gtccggcgag agccccaact ggatccggcg
     3961 cagttccacc cggtcggcca cgttctccgg cgccccgggc ccgcccccgg ccgctccgtc
     4021 cgatgatgtg cgcttctcca ccaccctcgc ctccttcacc ggggctctcg ataccgttcc
     4081 cagaggaaca cagggtttcg gagtcccgcg agcggggacc ggcgcttcgt cggccgaccg
     4141 cctgtacgtg gaggggggcc gggcccgcgc atcaccgtcg gcgctcagcg gcgcgccgtc
     4201 ccggttccct tccgcacctg cgggccggcc ggggccggcg tccccgaggc ttcggtgagg
     4261 tgggcgtacg cccggtcgag cagtgaggtg gccccgggac gcgcctggag cgcgggcagc
     4321 aggaggagcc acaggtcgtg cagcctctcc tcgatgtgtt cccggccgtc cagcgcgtcg
     4381 gagacggtgt gcaggccgaa gaacgcggac accacggcgt tccccgcctt ccgggggatc
     4441 acggacgggg tgagtacgcc ctcgcgaccg gcctcctcca gcaggccgac caccgtctcg
     4501 atccagccct cgaacggaga gggaagggac gtgtcgacgg acttgcgttc ggcccagagc
     4561 cgtgagccgc ctcgtaccac gacatcgtcc cggaaggcgc gggcgaccga gaaactcagc
     4621 gcgacgagct tctccagggc cgggatgtcc gcctcacggt ggcgtctgac cagcgtgggc
     4681 caggtgtcga acacctcctc catcaccgcg agggcgagat tctccttgca cgcgtaatgg
     4741 aagtagatcg caccgctcgt ccggccggag agcgcgctga tgtcactgat actcgtgccc
     4801 gcgtagcccc gctcctcgaa cagttgcgcg gccgcctcca gaagagaacg ccgtgttgcc
     4861 ttcgcccgtc cctgcacatg caccccgatg ccgcgccttc ccgtttcagc acggaaatct
     4921 agtccatgaa gccgtcgtga tcgaaaggac ggagtgcgga cctgaagggg atgagttctt
     4981 ctgttcatat ggagaattcg gtgggtgtat tcaccggtga atcccggctg agctggtcac
     5041 ggaccgtgcc tcgtgaactg gtgcaccgca ggtcggcggc ggaggtgctg ctcaccgacg
     5101 tacgtcccgg caggcagagg ggcgtgttcg acgcggcggc ctcctggtcg agatcacatc
     5161 ccacgtttcc ccgggacggt gccgacctgc acagtccgct gatcgtcatc gagacgatgc
     5221 gccagctcgg tatctatctg ccgctgcgtc attacgcggt gccgcccgaa tcccggctgc
     5281 tgatcaccga cctgttcttc cggatcgatc cggaacgcga accccgcgcc acctcgaatt
     5341 ccacggacgt gacctgccgc gcgcgagtga ccgacatacg caacggggcg gacggccatg
     5401 tcgccggact gcggatggag atctccgtcc gaaccggctc cgggatattc gcacgggccg
     5461 gcggcggggc gcgtttcctg ggcccggagc agtacgccgc cgtacgcggc gccggggcga
     5521 ccgcgcttcc gcccgcgccc accggccccc gtgaccggcc ggacatcgga cggatcgcgg
     5581 tggccgaccc gcgcgatgtg atgatcgcgc tcgacgacgg cgccgtacga ctgcggccgg
     5641 ccgatccgcg gcaccccttc ttcttcgacc acccctcgga ccatgtgccg ggcatggtcc
     5701 tgctggaagc ggcgcggcag gccggcgcgc tcgcgagcga gggccggtgt gtgcgcccta
     5761 ccgccggtcg gctgaaagcc ctgagcttcg ccgagttcgc gccggccgcc cgcgtgctgg
     5821 ctgtgccgca ccaccggacc tgcggtttcc gtgttctgca agggggccgg tatgtcgcct
     5881 atggggccct ggagtaccgg taggcacatt ttcataccta tgtgtccctc cttgactact
     5941 tttcgtaatg cttcataacg tagtgatcgt tatgttcttg ggtggacaag cccggagaaa
     6001 ccttccttcg tcatgtgcgt ccacccggaa ccgatcccgc ccccggatcc ggcggcctac
     6061 gcgtcgaccg tgccctgacg gcaccgtgct cggcgttctg tcctccgcgg gcactgcgga
     6121 acggaacaac tcgcatgacc tctgaacgag tcctctcctg gaccccctcg gccatcgtct
     6181 tcgactgtga cggcaccctc atggacaccg aaagacactg gcagaacgct cgagaacacg
     6241 cgctcggcga gttcggcctc gccccggctc cgggcttcgc ggaccgtgcc aagggactgc
     6301 actacaccca gtgcggcctg ctcatggccg aggaggccgg acggcccgac ctcgcggcgg
     6361 acatgaccgg cagcctgctc cggatcttcc gcgacctggt cgccgagaac cccctgacca
     6421 tgcccggagc ccgtgaactg gtcgtgtccg cggccacgtt cgccccgctg gccgtggcga
     6481 gcaactgccc gcgcgaggtc gtcgagtcct gcctggacac ggcgggcctg cgtgactact
     6541 tcggccacat cgtggtcccc gacaccggaa tccggcccaa gccgcagccg gacgtctacc
     6601 tggccgcggc acgccactgc gggacggacc cggccgacac gctcgccgtg gaggactccc
     6661 tgtgcggtgt cgaagccgcg tcacgggccg gtatgcgggt gctcggggtc ggcccctggc
     6721 ccggagagcg gaccacggcg atggtcgacc tgtgggtcag ctcgctcgcc gaaccggacc
     6781 tctcgcggtg ggccgccgca cgcatccccc ggcaggccac gcgctgacgc cctgcccagg
     6841 gccgcgcccc ctgccccgag tggggggccc gaacggcgcc cgtcccgcca cgggccgaca
     6901 ggcgcgagaa tagtcatatg agccctttcg ggaaatctgc tccggatttg cgcggccggg
     6961 tcggcggcgc tctcttcgcc cgggtcgcgg gcccggcggg acaagagaac cgggcgcgta
     7021 tccatgacac tccggggccg cggtggttcg aggccgacag gccggtccga accgtccacg
     7081 gcgacgcctc gatgttcatc ggcggcctca gagcgctgct cctgcagtcc ctccacccgc
     7141 tggcgatggc ggcggtggcc gctcactccg gctaccgcgg cgacccgtgg ggcagactcc
     7201 agcgcacgag tacgttcctg gccatgacga cgtacggaac cgccgacgac gcacaggcgg
     7261 ccgtcgaccg ggtgcgggcc gtgcacgagg gcatacgggg acggaccgcc tcgggggagg
     7321 cctaccacgc ggccgacccc catctgctgg gctgggtcca cgccgcggag gtggacagct
     7381 tcctcatggc ccaccagcgg tacggagcgg atcccctgga cggggcgggc ctcgacggct
     7441 acgtcgccga caccgctcac gtcgcggagg cgctcggagt cgtggaccct ccgcgtaccc
     7501 gcgaggagtt gaccgcggtg ctcacggcgt accgccccga actgcgcccc acccgcgagg
     7561 cgcttgaggc cgcccacttc atcctccacc acccgcccct gcccctcgtg gcccggccgc
     7621 cctacgcggt cctcgccgcg aacgcgatcg tcacgcttcc cccgtgggcc cgtaccgcac
     7681 tgcgggtgcg gcctccgccg ctgctgccgg agggttgtgt ggaaccggcc ggtcgcctgc
     7741 tcacccgcac cattcgctgg gccatgccgc cggccccgcc gctcaccacc tccggggcga
     7801 acgccgggtg acggcgggcg gccgggcatc cgggtcgggt ggcccgggtg gagggccggt
     7861 cgggtcggcc gtgcccgctc gatcccggcg gcccccgccc cgtccggtcg gcgcacggat
     7921 ccggacaggg cgcagaccgc accgcgggcc gagtctcaga tccgggtcat ggtcgaggtg
     7981 taaccccctc cgccggggta gacccagtcg ccggtcgccg tggtgccgtc ggcgctgaac
     8041 gtgccttcgt agtaggccgg gctgcccttc gcgccggccc agatcgtcag cttgtcgccg
     8101 gccagttcgt agacgtagtc gaaggtgttg ccgttcgagt cgtagtaccg cgacaccacg
     8161 tccgtgccga ccggctcgcc gaacgggcgg agattcccga tcagttccat gccggtgacc
     8221 ggctcgccgg actgggtgag ttcgacgtgc tggatcagga agaaccggcc gggcatccac
     8281 tcgtaccgca cggttccctc ggctcctccg gtcaccgccc aggtgccgac caggcggtcc
     8341 agggctctca gttcggcgct cggcaggttg gtctcggaca tttcttcctc cttgaagggt
     8401 tctcgcggct acctcggagc tgtccgcccg ttcttgacac ccgtctcatg tggtgtgggt
     8461 cacattgccg acgcgtcgag atcccggacc cggcaacgag gtagccgcga gaacccctca
     8521 aggaggaaga aatgaccgtt cccgccgttc ccgccgcccc cgctgtttcc accaccggcg
     8581 tcgtttccac ggcccgctcc acggcccgct cctcgggccg cctgctggcc gccggggccc
     8641 tcgccggacc ggtcttcgcc gcttcggccg tcatccagat gttcacccgt acgggcttcg
     8701 acatcgcccg gcacccgatc agccagctct ccaccggcgg cctcggctgg atccagatca
     8761 cgacattcgt gctcgcgggt ctcggcgcac tcgcactggc cgccggtatc gggcgcactc
     8821 tccgcgaggg atacggacgg cgggtgctgc cggtctgtgt cgggatcttc ggcgtcgggc
     8881 tgatcgccgc cggtctgttc cccatggatc cggagaacgg cttcccggtc ggcacccccg
     8941 accggccggt cgccgagatg tcctggcacg gcgtcgcgca ctccgcgtcg gccgccatcg
     9001 ccttcacggc actggccgtc gccgccatcg tcctgaccgt acggtgtgtg cgccgccacg
     9061 cggtgctccc ggccgtactg aacggcgccg ccgcgctcgt cctggtcctg cccatggcgc
     9121 cggaccacat gagtatccag atcgccgtga acggtctggt cgccttcacc tggacgaccg
     9181 tcgtcgcgat gtcactgcgg cgcacccgct gaaccgccct accatcaccc gaacaggatc
     9241 caaggggaaa ccgcgatgaa gtatctgctg ctcggctaca catcggccgc cgcctgggac
     9301 gccgcgaccg ccgattcccc gtccccggag gcgctggccg cgttcgccgt ctaccagagg
     9361 ttcgagcggg agctgatcga caccggtgag ctcgtcacca ccgagggcct gggccacccg
     9421 gcggtcagca ccacggtcca ccggacaccg gacggcatga tggcgaccga cggaccgttc
     9481 gccgaactga aggaggttct ggcgagcttc gccgtgatcg acgtggccga ccgggagcgg
     9541 gcggtggaga tcgcctcgcg gatcgtggag gtgctcgggg agccgatcga gatccggccg
     9601 atcatgagcg aggacttcac cgcatgaccg gcggaccccc acgaggcccg gtgtgcgcgg
     9661 cgcgccacgc gcttcggtgc gggacgggcc ggtgaacggc gggccggacg cggacatcga
     9721 cacggccacg gccacggcca cggacatcga gaatctgctg cgtgccgaag caccgcaggt
     9781 gctcggcgcg ttggtgcggc gcttcggccg cttcgacacc gccgaggacg cggtacaaga
     9841 agcgctgctg gtggcgagca ggacatggca ggccgagggc ataccggagg acccacgcag
     9901 ctggctcatc cggatcggct accggcggat ggtcgacctg ctccgctccg accgggcccg
     9961 gcgccggcgt gagcgggagg tcggaatgac cgaactggcc atgggggaaa cggatcgccg
    10021 ggccggaccc ccgcaggaga ccgacgacag cctcaccctg ctgctcctgt gctgccaccc
    10081 ggcgctgagc cacgcctcac aagtggcgct cacactgcgc gcggtcggcg gtctgacgac
    10141 agccgagatc gcgcacgcct acggaacgtc cgaaaccacc atgggcacgc ggatcagccg
    10201 cgccaaacag caactcatcc gcgccggcgc ccacttcacc gcgcccaccg ccgccgaccg
    10261 gcccggccgg atgacggccg ccatgcgggt gctgtatctg atcttcaacg agggctatac
    10321 ggccaccacc ggcgacgaac tcgcccgtgt cgacctgacc ggtgaggcga tccggctgac
    10381 ccggactctc gccgacacca tgccggacga cggagaggtg accggcctgc tggcgctgat
    10441 gctgctcacc gagtcgcggc gcgcggcccg caccggtgac gacggcggtc tgctgccact
    10501 ggacgagcag gaccgcacgc gatggaacgg ccatctcatc cgcgagggaa ccgagttgat
    10561 cgacagggtc tggaacggga gcgggacggg cccctaccaa ctccaggcgg cgatcgcggc
    10621 gttgcacgcc gccgccacga ctcctcagca cacggactgg ccgcagatcg ccatgctgta
    10681 cctgtggctc gaacgcctca ccccgacccc gccggtacgg ctgagccggg tggtcgccgt
    10741 ggcccaagcc ttcggaccgg cccgggggtt gacgctgctc gacgacctca accgccgcca
    10801 cggactggac cgggcccctc tcacccggca acgcgaacgc gcggtgcgcg cccacctggt
    10861 ccagatgacc ggcgacacgg cgggcgccac gacgctgtat cgc
//
