LOCUS       NZ_CP048397            10447 bp    DNA     linear   CON 08-MAY-2024
DEFINITION  Streptomyces sp. S4.7 chromosome, complete genome.
ACCESSION   NZ_CP048397
VERSION     NZ_CP048397.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. S4.7
  ORGANISM  Streptomyces sp. S4.7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7920066)
  AUTHORS   Bekiesch,P., Zehl,M., Domingo-Contreras,E., Martin,J.,
            Perez-Victoria,I., Reyes,F., Kaplan,A., Ruckert,C., Busche,T.,
            Kalinowski,J. and Zotchev,S.
  TITLE     Viennamycins: new lipopeptides produced by Streptomyces sp. S4.7
            isolated from the rhizosphere of Edelweiss (Leontopodium nivale
            subsp. alpinum)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7920066)
  AUTHORS   Bekiesch,P., Zehl,M., Domingo-Contreras,E., Martin,J.,
            Perez-Victoria,I., Reyes,F., Kaplan,A., Ruckert,C., Busche,T.,
            Kalinowski,J. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2020) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.8x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010384365.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 10:27:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,027
            CDSs (total)                      :: 6,939
            Genes (coding)                    :: 6,650
            CDSs (with protein)               :: 6,650
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 103 of 289
            Pseudo Genes (incomplete)         :: 223 of 289
            Pseudo Genes (internal stop)      :: 23 of 289
            Pseudo Genes (multiple problems)  :: 52 of 289
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:05:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4081452
            Orig. end                         :: 4091899
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048397.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zotchev Lab, University of
            Vienna, Austria.
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10447
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="12"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10447
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10447
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5447](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5447
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    30..45
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.44"
                     /tool="antismash"
     misc_feature    complement(219..234)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.74"
                     /tool="antismash"
     gene            284..583
                     /locus_tag="SSPS47_RS36255"
                     /old_locus_tag="SSPS47_18425"
     CDS             284..583
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /locus_tag="SSPS47_RS36255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_18425"
                     /product="hypothetical protein"
                     /protein_id="WP_343234926.1"
                     /transl_table=11
                     /translation="MAWEVPTLGDLRIVAERLSKRGALVGATDHGTTKGVYAKDPDGLE
                     FEVCWVLPAHLVDDEARARRGELDIDAEIVRYGATTQGACARAAPRTPIPPGFA"
     gene            993..1151
                     /locus_tag="SSPS47_RS18170"
     CDS             993..1151
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SSPS47_RS18170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_164252008.1"
                     /transl_table=11
                     /translation="MAELQANLLRLDAAGCRTLIRWENDRRRFGLTLGELATASAYEPL
                     ARVQLLS"
     gene            1277..1633
                     /locus_tag="SSPS47_RS18175"
                     /old_locus_tag="SSPS47_18430"
     CDS             1277..1633
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SSPS47_RS18175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SSPS47_18430"
                     /product="hypothetical protein"
                     /protein_id="WP_239064961.1"
                     /transl_table=11
                     /translation="MERDLKRHLAWYAEKDPNGSLFVGEKGAPFRRSGFGRKWRRARTK
                     VGLPDNFRFYDLRHTGHTLSTQSGATLKDTMVRAGQSSEKAALIHQHSTGNVRWRPPRD
                     RRSRTHGAGEGQET"
     misc_feature    1686..1701
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 24.13"
                     /tool="antismash"
     tRNA            complement(1737..1810)
                     /anticodon=(pos:complement(4083228..4083230),aa:Gly,
                     seq:ccc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SSPS47_RS18180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SSPS47_18435"
                     /product="tRNA-Gly"
     gene            complement(1737..1810)
                     /locus_tag="SSPS47_RS18180"
                     /old_locus_tag="SSPS47_18435"
     gene            2243..2818
                     /gene="dcd"
                     /locus_tag="SSPS47_RS18185"
                     /old_locus_tag="SSPS47_18440"
     CDS             2243..2818
                     /EC_number="3.5.4.13"
                     /GO_function="GO:0008829 - dCTP deaminase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="dcd"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948066.1"
                     /locus_tag="SSPS47_RS18185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_18440"
                     /product="dCTP deaminase"
                     /protein_id="WP_147873493.1"
                     /transl_table=11
                     /translation="MLLSDKDIRAEIDSGRVRIDPYDESMVQPSSIDVRLDRFFRVFEN
                     HRYPHIDPAVEQVDLTRTVEPAGDEAFILHPGEFVLASSFEVISLPDDIASRLEGKSSL
                     GRLGLVTHSTAGFIDPGFSGHVTLELSNLATLPIKLWPGMKIGQLCLFRLSSPAEFPYG
                     SERYGSRYQGQRGPTASRSFLNFHRTQV"
     gene            2824..3345
                     /locus_tag="SSPS47_RS18190"
                     /old_locus_tag="SSPS47_18445"
     CDS             2824..3345
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658995.1"
                     /locus_tag="SSPS47_RS18190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_18445"
                     /product="phosphoribosyltransferase"
                     /protein_id="WP_164252009.1"
                     /transl_table=11
                     /translation="MDDIRENLTYERFGVAVRELAQAVADDGYEPDVVLSIARGGVFVA
                     GGLAYALGCKNIHLVNVEFYTGVGTTLAMPVMLAPVPNVVDFSNKKILISDDVADTGRT
                     LKLVYDFCLDHVAEVRSAVIYEKTHSLVKCEYVWQRTDEWINFPWSALPPCSKSDTSVA
                     LPGDMREKRS"
     gene            3435..4370
                     /locus_tag="SSPS47_RS18195"
                     /old_locus_tag="SSPS47_18450"
     CDS             3435..4370
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374554.1"
                     /locus_tag="SSPS47_RS18195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_18450"
                     /product="transposase family protein"
                     /protein_id="WP_203557751.1"
                     /transl_table=11
                     /translation="MVGNSARALVISNRRITGLTADVIAELVAEVGPLWHERHQARLAS
                     RPRKRAMGAGAKHRLVFVDRLLATLVHLRHGTTHDVLACWFGVDRSTITRAINEVRPLL
                     AERGCTISPDVRLRTLAEVVDHLGATGKTGIIDGTEIRVRRPAQGRKDRDKFISGKNKQ
                     NAVKSMVVTDGEGRMLWCSPTKPGSCADITHARQLGLVELLAGGPAVEILADAGYQGLG
                     AQTGGRVVTPPHRKFKKNAPDWYEDMYERQRKAHSSRRIRVEHGIAHLKNWRALARHLG
                     RREHMSDTVQAIAGLLSHQQGADLTSARQM"
     misc_feature    4738..4751
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.24"
                     /tool="antismash"
     gene            5001..5447
                     /locus_tag="SSPS47_RS18200"
                     /old_locus_tag="SSPS47_18455"
     CDS             5001..5447
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950154.1"
                     /locus_tag="SSPS47_RS18200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_18455"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_164252010.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.7e-11,
                     bitscore: 43.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISGRSVVFTLASAVLAVGALAAPAAAEDSPISRIVVHTAGDGS
                     QPPAELGNPKEWGVVTLALDDLAGEFTPKTIIEVGGGTWSYGWEARNDQKYCYSNYYHR
                     TVDHGSTVKIANGTVKAVAFAGQTSNANRSAGFAYTCETYYAKY"
     gene            5609..6250
                     /locus_tag="SSPS47_RS18205"
     CDS             5609..6250
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712128.1"
                     /locus_tag="SSPS47_RS18205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_239064962.1"
                     /transl_table=11
                     /translation="MYFLRIRVMPIVIVFTVGVVLGVMGPLMGKFDNPVCHAAALVFSG
                     GWAWACFAFLVGFSRRSKIESALLASSALAVAVVVYYVFKSMFPASPIGLVVSSGAGDG
                     LGSKILVWGIAAFVFGAPVGLFGNIARTRGIGGLPFRLLIPLTAFIETSARLDAEAPSQ
                     GDVVEFTWNAIRIAAGAAFLAVLLHELITWRAERVAPGEERADNQAVIGN"
     misc_feature    complement(5641..5656)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.19"
                     /tool="antismash"
     gene            complement(6456..7793)
                     /locus_tag="SSPS47_RS18210"
                     /old_locus_tag="SSPS47_18460"
     CDS             complement(6456..7793)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1229: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015654835.1"
                     /locus_tag="SSPS47_RS18210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_18460"
                     /product="IS630 family transposase"
                     /protein_id="WP_239064963.1"
                     /transl_table=11
                     /translation="MASRGPRAVEVVLSAEERAELARWAGGAVSARAAERARIILACAE
                     GASNTGVAADFGVSAETVRKWRSRFVARRVAGLVDKPRPGRRKPELVLSEAERAELTRW
                     ARRAKTAQFLALRARIVLRCANGGTNKEIAAELGVSQGTVNRWRSRFIRLRLDGLTDEP
                     RPGRPPSILLDQVEDVLTATLESTPGKDTHWSRASMAKHSGLSRSTVGRIWKKFDLKPH
                     LQDAFKLSTDPQFVAKVVDVVGLYHHPPERAVVLCVDEKSQIQALDRSQPVLPMMPGMP
                     ERRTHDYYRHGITSLFAAFNIADGSVISQLHRRHRAIEFKKFLVTIDKAVPAGLDVHLV
                     CDNYATHNTAEIKTWLGKHPRFHVHFTPTGSSWMNQVERWFGLLTDKLIRRGVHTSLKA
                     LEQDIRAWIDGWNENPRPFTWTKTADEILNSLAGYLAKINPPATET"
     gene            7896..8120
                     /locus_tag="SSPS47_RS18215"
                     /old_locus_tag="SSPS47_18465"
     CDS             7896..8120
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SSPS47_RS18215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SSPS47_18465"
                     /product="hypothetical protein"
                     /protein_id="WP_343234892.1"
                     /transl_table=11
                     /translation="MLIRGVRLDPLITPTRRVPTGRAPARLAAWDLGVDEYGQSVDVSL
                     SQVPGVTVAGLPGFGKTSLIEVPPLLGRC"
     gene            complement(8121..8921)
                     /locus_tag="SSPS47_RS18220"
                     /old_locus_tag="SSPS47_18470"
     CDS             complement(8121..8921)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /locus_tag="SSPS47_RS18220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_18470"
                     /product="IS3 family transposase"
                     /protein_id="WP_164254683.1"
                     /transl_table=11
                     /translation="MRAAAPRRRLSKKIKAIHTGRWHLRVATGARHTETRGRHVGRKRV
                     ERLMREADLAGLSPRRAVSRAGIPSRARPDLVSRDFTAQGPNRLWVTDLTMIPTAEGPL
                     WLSAIRDAFSRRAVAWETSARADADLVLSSLEYAPASREVEPGKLIHHADHGCQYTSIK
                     LTTRLMRAGIDASMGSAGDSYDNALAENLWMLTKTECVRGRVFTTRAEANLALFEYFDG
                     FYNSRRIQKRLGYLSPIEFEEKHYAEQATTKRANLKPRQPSLTS"
     gene            complement(9028..9336)
                     /locus_tag="SSPS47_RS18225"
                     /old_locus_tag="SSPS47_18475"
     CDS             complement(9028..9336)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1164: transposase
                     IS3/IS911 family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /locus_tag="SSPS47_RS18225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_18475"
                     /product="transposase"
                     /protein_id="WP_164247473.1"
                     /transl_table=11
                     /translation="MAAPRKYSLELRERAVRMYRASDPKPQIKKLAVDLGVHPEALRGW
                     IRQAEADAGERDDRLTTDEHAELVALRKENAQLKRANDVLRTASAFFAAQLDPTRPR"
     gene            <9407..10195
                     /locus_tag="SSPS47_RS18230"
                     /old_locus_tag="SSPS47_18480"
                     /pseudo=""
     CDS             <9407..10195
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019324612.1"
                     /locus_tag="SSPS47_RS18230"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SSPS47_18480"
                     /product="cell division protein FtsK"
                     /pseudo=""
                     /transl_table=11
                     /translation="INGLVCDWAPSPAMQFVAFDGKVSAAYQGDYADLVKRVFAFVGDD
                     LEDANALFQRLVNLRETRHTAVKARLGVTDMWAVGPSAAWPLVVVIIDEAHTYFRDHRG
                     SDPKTKKLAALAAENARLVEDLVKKGRSVGILTVLATQKSTGDAIPTFIRDVCPVGLSF
                     AQKTAEAAVAALGEDIRNWDDANPINLQDPAYVGVASMSHQSRPGFARIRTPYVTDADS
                     ARIAEATAHLTQDPMELLRAHGGLTLSKAVDHPGDRPKAA"
ORIGIN
        1 accaccccgg ccgggttcaa gaaccaccga ctggtctgga gcagcgggcc ggtccgaggg
       61 ctcaatcacg cggtgctgtg ggtgcgcgac gcggacgcca gcacccgttt ctacacggaa
      121 accctcggac tccgggtggt cgtcgaatcc ggagaggccc ctcggaaggc gattttttct
      181 ccgggcaccg gaatccgaca atgaccacga tctcggcctg ttcggcatcg gcgcacaggc
      241 cgaagagacg agggccggtc ggggcgagac cggcctttac catattgcct gggaggtccc
      301 gacacttggc gacctgcgca tcgtcgccga gcggttgtcc aaacgcggag ccctggtcgg
      361 cgccaccgat cacggcacca ccaagggcgt ctacgccaaa gacccggacg gcctggagtt
      421 cgaggtctgc tgggtgctac ccgcgcacct cgtcgacgac gaggcccgcg ctcggcgcgg
      481 cgaactagac atcgatgccg agatcgtccg ctacggcgcc accacacaag gcgcctgtgc
      541 ccgtgctgcc ccccgcacgc ccatcccacc cgggttcgcc tgacgggctg ccccggagtc
      601 ctgctccacc ggagccggga cggaggagaa ggggggtggg cgccccggac agccgcaggg
      661 tcgggggctg tccggctgtc cacccgcggg tatgtccgtt cgtctgctcg gtgtctgccc
      721 gcagccgctg atcaccactc cgccggaatc gcccgtcgca tcggcagccc tgctgactgc
      781 gggccgacgc cctccttgac tcccccgatc tcgaaggcgt cctcccctcc cgacacttcg
      841 acgccgcctg ggccggccac ccgagcaggg cgccttggcg taaaatcttc gaccgccaca
      901 gcagcggcga tcggctgcgg ccgaggccag gtgagccgaa gcccgagtgc gacgcggaca
      961 ccacgacgct gacgggccga tgtcaggtga aggtggccga gctgcaggcg aacctgctgc
     1021 ggcttgacgc cgcgggctgt cgcacgttga tcaggtggga gaacgaccgc cggcggttcg
     1081 gtctgacgct cggcgaactc gccactgcca gcgcctacga gcccctggct cgggttcagt
     1141 tgctgtcctg atctggcacc gccggctcgg ggtggacctg atggtgccgc tggtggacag
     1201 ctcatgggtg cgcagcccag ggaacgatga cacgaagtcg gcaaccggtc ggcgcttcct
     1261 cgtgctaccc ggcttcatgg agcgcgacct gaagcgacac ctcgcctggt acgcggagaa
     1321 ggacccgaac gggtcgctct tcgtggggga gaagggtgcc ccgttccggc ggtccggctt
     1381 cgggcggaag tggcgcaggg cgcgcacgaa ggtggggttg cccgacaact tccgcttcta
     1441 cgatctccgc cacaccgggc acaccctgtc gacacagtcc ggcgcgacgc tgaaggacac
     1501 gatggtgcgg gccgggcagt cgtcggagaa ggcggccctc atccatcagc actccaccgg
     1561 gaacgtcaga tggaggccgc cgagggatcg acgctcgcgt acgcacggag cgggagaagg
     1621 ccaagaaacc tgacactggc ccggacgatc aggaatcggg cacggcacgg gcacgagacg
     1681 cctagagagg tctagacaac aaaggaggcc caagtcgctg acttgggcct ccttcatgga
     1741 gcgggtgacg agaatcgaac tcgcgctctg agcttgggaa gctcatgttc taccattaaa
     1801 ctacacccgc ctggagcgcc gatgatcatc cgcgcttccg tggacactct accttattgt
     1861 cgactccgcg tgctcgatgg cgctgtcggt cgcgggagtt ggggcgtagc gtccagggtc
     1921 ggagtgccgc ttccgtcggt ggttcgttga tcccctaatg tgtgggttcg tccgcgtttt
     1981 ggggaaggga cctgatggat ttgatggagc gcaccgtcgt ccgctgtgcc gaagggcatg
     2041 tgttcagtac tacgtcgttt ccgatgcagc agcttgacaa cgggcggctc gggcccgggc
     2101 ggctcattcg gtgtccgcgt tgtgcgcggt tgcggcatgc cgtacccgtg gaggtcgggc
     2161 ggcgctgacg cgggtggtcg gtaggggcgc gcggggttgc ccgagttggg gtgggcccgc
     2221 gcgttctgcg tatcctcggg gcgtgcttct ctcagacaag gacatccggg ccgagatcga
     2281 ttccgggcgc gtacgtatcg acccttacga cgaatcgatg gtgcagccgt cgagcatcga
     2341 cgtgcggctc gaccggttct tccgtgtgtt cgagaaccac cgttatccgc acatcgaccc
     2401 cgccgtcgag caggtcgacc tgacgcggac cgtggagccg gccggggacg aggcgttcat
     2461 cctgcacccc ggtgagttcg tgctcgcctc gtcgttcgag gtcatctcgc tgcccgacga
     2521 catcgcctcc cggctggagg ggaaatcgag tctcggccgt ctggggctcg tcacgcactc
     2581 gaccgccggt ttcatcgacc ccggcttctc cgggcacgtg accctggagc tgtcgaatct
     2641 cgcgaccctg cccatcaagc tctggcccgg catgaagatc gggcagctgt gtctgttccg
     2701 gctcagctcg ccggcggagt tcccgtacgg ctcagagcgc tacgggtcgc gttaccaggg
     2761 gcagcgcgga cccaccgcct cccgctcgtt cctcaacttc caccggacac aggtgtgaag
     2821 gacgtggacg acatacggga gaacctcaca tacgagcgct tcggcgtcgc cgtgcgggag
     2881 ctggcgcagg ccgtcgctga cgacgggtac gagccggacg tcgtcctgag catcgcgcgc
     2941 ggcggggtgt tcgtcgccgg cgggctcgcc tacgccctcg gctgcaagaa catccacctg
     3001 gtgaacgtgg agttctatac gggagtcggc acgactctcg cgatgccggt catgctggca
     3061 ccggtaccga acgtggtgga cttctccaac aagaagatcc tgatctcgga cgacgtcgcc
     3121 gacaccgggc gcaccctcaa gctcgtttac gacttctgtc tcgatcacgt cgccgaggtc
     3181 cgcagcgcgg tcatctacga gaagacgcac tcgctggtga agtgcgagta cgtctggcag
     3241 cgcaccgacg agtggatcaa cttcccctgg tctgctttgc ctccatgcag caagtctgac
     3301 acatcggttg ccctgccggg cgacatgagg gaaaagcgct cctgaactgc acttcagcct
     3361 ctcgtggtgg ggctaacgag ctcgtgcatg gttggcggtg agacgccgag tccttgatcg
     3421 ttgatcatgg ttgcgtggtg gggaactcgg ctcgtgcact ggtcatcagc aaccggcgga
     3481 tcacgggcct gacggccgat gtgatcgctg aactcgtcgc cgaagtgggt ccgttgtggc
     3541 acgagcgcca ccaggccagg cttgcctccc ggccgcgcaa gcgggccatg ggcgccggcg
     3601 cgaagcaccg gctggtgttt gtcgaccggc tgctggccac tctcgtccat ctccgtcacg
     3661 ggactaccca cgacgtgctg gcctgctggt ttggcgtgga ccgctccacc atcacccggg
     3721 ccatcaacga ggtgcggccc ctgctcgccg agcgagggtg caccatcagc cccgacgtac
     3781 ggctgcggac tctggccgaa gtcgtcgacc atctcggcgc gacagggaag accggcatca
     3841 tcgacggcac cgagatccgg gtccggcggc cggcccaagg acgcaaggac cgggacaagt
     3901 tcatctccgg caagaacaag cagaacgccg tcaaatccat ggtggtcacg gacggcgaag
     3961 gacgcatgct gtggtgcagc ccgaccaagc ccgggagttg cgcggacatc acccacgcac
     4021 gccagttggg gctggtggag ctcctggccg gtgggcctgc ggtcgagatc ctcgccgatg
     4081 ccggctacca ggggctcggc gcacagaccg gcggacgcgt ggtgacgcca ccgcaccgca
     4141 agttcaagaa gaacgccccg gactggtacg aggatatgta cgagcgccag cgcaaggcgc
     4201 actcctcacg ccgtatccgg gtcgagcacg gcatcgcaca tctcaagaac tggcgggcac
     4261 tcgcacgcca cctcggccgc cgcgagcaca tgagcgacac ggtccaggcc atcgccggcc
     4321 tgctgtctca tcagcagggc gcagacctga catcggcacg gcagatgtga cccccgggcc
     4381 ccaccgaagt cctcggcgtc tcaccgccaa ccatgcacga gctcgttagc tagtgcccac
     4441 acgtgatgtg gaagccagag catgtgatcg acagcctctg tacgtcatcg ttgccgtcaa
     4501 ctggggcatg gagtagacga tggcgtgact cgatccggcg ggccgttgat cagctgatcg
     4561 aagctcgctg aacggttagc gctgccccac ggaacgggct aaaccgccca tgtcaccact
     4621 gggtgaacga acccgaagca ggtcgctgcg aggccggacc atcatgaccc cgacagcagc
     4681 ccctcctcgt tacgcctacg tgcgcctcca agagccacgc tgcctctcgt gcagatggtg
     4741 actctacgtg cctaattagc cactggaagt gtgtggctgg gggcgtaacg ccagcgtcaa
     4801 ggttgccaac atgaaggcaa ttcaagtgga ttatgcatcc aaaaaggaca ctcccgggtc
     4861 ggaagattta aaaactgatc aacccaaaat cgggcgaagc gatttcaggt cgacctcttt
     4921 ggtgcgcctc gcctttgggt gcagacctgc gctggatgta tgcgcagccc caacgtgggg
     4981 gaatgattcg aggagttttc gtgaagatct ctggcagatc cgtagttttc acccttgcga
     5041 gtgcggtcct ggctgtcggc gctctcgccg ccccggccgc cgcggaggat tctccgatca
     5101 gcaggatcgt tgtgcacacc gccggggacg gctctcagcc gcctgcggaa cttggcaacc
     5161 ccaaggagtg gggtgtggtc acgctcgcac ttgacgactt ggctggcgaa tttacgccca
     5221 agaccatcat cgaagtcggt ggcggaactt ggagctacgg ctgggaagcc aggaacgatc
     5281 aaaagtactg ctactcgaac tactaccaca ggaccgtgga ccacggctca actgtgaaga
     5341 tcgccaacgg cacggtaaag gccgtggcgt tcgccggcca gacttccaat gccaaccgtt
     5401 cggctggctt tgcctacacg tgtgagacct actacgctaa gtactagtct tgatctttcc
     5461 ggcgctttgg gagtgtgtta taggtgcggc gtcagggtgg ccggggggaa agctagtgcg
     5521 atcggggctc tgcgactgtg tacgtagagc cccgattggc gaaatgcttg gccttggtca
     5581 tggaccacag aagcgggata ttttaatcgt gtactttctg cgtatccgtg tcatgcctat
     5641 cgtgattgtc tttacagtcg gcgtggtttt gggtgtgatg ggcccgctca tgggcaaatt
     5701 tgacaacccg gtctgccatg ctgctgcgct cgtcttctct ggtgggtggg cgtgggcctg
     5761 cttcgccttt ctggtgggtt tttcgcgccg gtccaagatt gagtcggcgc tgctggcttc
     5821 ctcggcattg gctgtagccg tggtcgtgta ctacgttttc aagtctatgt ttccggcttc
     5881 ccctatcggt ctggtcgtct cctctggagc gggagacggg cttggttcta agatccttgt
     5941 ctggggaata gcagccttcg tctttggggc ccctgtgggg ctatttggga acattgcgcg
     6001 aacgcgtggt atcggaggac tcccgtttcg actactcatc ccgttgactg catttatcga
     6061 gacctctgcg cggttggatg cggaggctcc atcgcaaggt gatgttgtcg agttcacctg
     6121 gaacgcgatt cgaattgcag cgggtgccgc tttcttggct gtactgctgc acgaattgat
     6181 aacttggcgt gctgagcgcg tggcgccagg agaagaacga gcggataacc aggctgtcat
     6241 aggaaattaa gtatcttctc cctgcggctc ggctcatagg tatagccgtg atcggtttag
     6301 ctttgccacg ggcaagcccg atattgcgat agagtgcagg tgtgcagtgt gatcggaaat
     6361 tcgaaacccc actcggacgg cgtccgagtg gggcgttttg ctatctgcgc atgcgtctag
     6421 tgtgatgcgc cagaaatgat gagactaagt ctgtttcagg tttcagtggc cggcgggttg
     6481 attttggcga ggtagccggc gagggagttg aggatctcgt cggcggtctt ggtccaggtg
     6541 aagggcctgg gattctcgtt ccatccgtcg atccaggccc tgatgtcttg ctccagggcc
     6601 ttcagggagg tgtgcacccc gcggcggatg agcttgtccg tcagcaggcc gaaccaccgc
     6661 tcgacctggt tcatccagga ggagccggtg ggggtgaagt ggacgtggaa acgggggtgc
     6721 ttgcccagcc aggtcttgat ctcggcggtg ttgtgggtgg cgtagttgtc gcagaccagg
     6781 tgcacgtcga ggccggccgg cacggctttg tcgatcgtga ccaggaactt cttgaactcg
     6841 atcgcccgat ggcggcggtg cagttgcgat atgacgctgc cgtcggcgat gttgaaggcc
     6901 gcgaacaggc tggtgatgcc gtgccggtag tagtcgtggg tacgccgctc gggcatgccc
     6961 ggcatcatcg gcagtacagg ctgcgagcgg tccagcgcct ggatctggct cttctcatcc
     7021 acgcacagca ccaccgccct ctcgggcgga tggtggtaga ggccgacgac atcgacaacc
     7081 ttggcgacga actgcgggtc ggtggagagc ttgaaggcgt cctggagatg gggcttgagg
     7141 tcgaacttct tccagatccg cccgacggtg gaccgcgaca gcccggagtg cttcgccatc
     7201 gaagcccgcg accagtgggt gtctttgccc ggggtggact ccagcgtcgc cgtgagcacg
     7261 tcctcgacct ggtccagcag gatcgagggc ggcctgccgg gacgcggctc atcggtcagc
     7321 ccgtccagcc gcaaccggat gaacctcgac cgccagcgat tcaccgttcc ttgcgataca
     7381 ccgagttcgg cggcgatctc cttgttcgtc ccgccgttcg cgcaccgcag cacgatcctc
     7441 gcccgcagcg ccaggaactg cgcggtcttc gcccgccgcg cccagcgcgt cagctcggca
     7501 cgctcggcct cggagagcac cagttccggc ttgcgccggc ccggccgcgg cttgtccacc
     7561 agccccgcca cccgccgggc cacgaaccgc gagcgccact tgcgcaccgt ctccgcagac
     7621 accccgaagt ccgctgctac accggtattt gacgctccct ccgcacacgc cagaatgatc
     7681 cgggcccgct ccgccgcacg ggccgacacg gcaccacccg cccaacgcgc cagctctgcc
     7741 cgctcctcgg cagacagcac gacttccaca gcacgaggac cccgagacgc cataaaaaca
     7801 gcctacagac ttagtctcat catttctggc gcatcacagt agcggacgca tggcggtgta
     7861 cgcgggtgtc ggtccttccc gatggacccg gccacgtgct cattcggggg gtgcggctcg
     7921 atccgctgat cacgccgacc cggcgggtgc ctaccgggcg ggcgcctgcc cggctggcgg
     7981 cctgggacct gggggtggac gagtacggac agagcgtgga cgtgagcctg tcccaggtgc
     8041 ccggtgtgac cgtggccggc ctgcccggct tcggcaaaac cagcctgatt gaggttcccc
     8101 cgttgctcgg gaggtgctga tcagctggtc agggagggtt gacggggttt caggttcgct
     8161 cttttggtcg ttgcctgctc ggcgtagtgc ttctcctcga actcgatcgg gctgaggtag
     8221 ccgagccgct tctggatgcg ccggctgtta tagaagccgt cgaagtactc gaagagtgcg
     8281 aggttggctt cggccctggt ggtgaagacg cggccgcgga cgcactcggt cttcgtcagc
     8341 atccacagat tctccgcgag ggcgttgtcg taactgtcgc cggccgagcc catcgacgcg
     8401 tcaattcctg ccctcatcag gcgtgttgtc agcttgatgg acgtatattg gcagccgtgg
     8461 tcggcgtggt ggatgagctt gccgggctcg acctcgcggg aggcgggcgc gtactccagg
     8521 ctggacagga ccaggtcggc gtccgcgcgg gcggaggttt cccaggccac ggcccggcgg
     8581 gagaatgcgt cgcggatcgc ggacagccac agcggtccct cggcggtcgg gatcatcgtc
     8641 agatcggtga cccacagacg gttcggcccc tgggcggtga agtcccggct gacgaggtcc
     8701 gggcgagcgc ggcttgggat cccggcgcgt gaaaccgccc ggcgcgggct gagacccgcc
     8761 agatcggcct cgcgcatcaa ccgctcgacg cgtttgcggc ctacgtgacg ccctcgcgtt
     8821 tcagtatggc gtgcacccgt ggcgacccgt agatgccacc gaccggtatg gatcgccttg
     8881 atcttctttg agagtcgacg tcgcggcgcc gccgctcgca gggctctttc tcagcgcggc
     8941 gccagcggta gtaggtggac gaggcgatgg aaagctcccg gagtacgcac tcgactccca
     9001 ggtgtggatg ctcgtcgagg agcgcggtca cctgggccgg gtcgggtcga gttgcgccgc
     9061 gaaaaacgcc gaggccgtcc gcagcacgtc gttcgcccgc ttgagctggg cgttctcctt
     9121 ccgcagtgcc accagctcgg cgtgttcgtc ggtagtcagc cggtcatccc gctcgccggc
     9181 atcggcctcg gcctgccgga tccagccccg cagggcctcg gggtggacgc caaggtcgac
     9241 ggccagcttc ttgatctgcg gcttcggatc cgacgcgcgg tacatccgca ccgcacgctc
     9301 acgcaactcc agtgagtact ttctgggggc agccatggac gatgttcctc tcatgagaac
     9361 catctgaccc tctgtcatcg acttccgcat ctcgggggaa cctcagatca acgggctcgt
     9421 ctgtgactgg gcgccttcgc ccgcgatgca attcgtcgcc ttcgacggga aggtgtccgc
     9481 cgcgtaccag ggcgactacg cggacctggt caagcgcgtg ttcgccttcg tcggtgacga
     9541 cctggaagac gccaacgccc tgttccaacg gctggtcaat ctgcgcgaga cccgccacac
     9601 ggccgtcaaa gcccgactcg gggtgaccga tatgtgggcg gtcggcccgt ccgcggcgtg
     9661 gccgctcgtc gtggtgatca tcgatgaggc acacacctac ttccgtgacc accggggcag
     9721 cgaccccaaa acgaagaagc tcgcggcgct ggcggcggag aacgcccggc tcgttgagga
     9781 cctggtgaag aagggccgct cggtgggcat cctgaccgtc ctcgccacac agaagtccac
     9841 gggcgatgcc atccccacct tcatccgtga cgtctgtccg gtcggcctgt cgttcgcgca
     9901 gaagacagca gaggcagcgg tggccgcgct gggcgaagac atccgcaatt gggacgacgc
     9961 caatccgatc aacctccagg accccgccta tgtcggcgtc gcgtccatga gccaccagtc
    10021 ccgccccggc ttcgcccgca tccgcacccc gtacgtgacc gatgccgact cggcccgcat
    10081 cgccgaggca accgcccacc tcacccaaga tccgatggaa ctcctgcgtg cgcacggcgg
    10141 gctgaccctg tccaaggccg tcgaccatcc cggagacagg cccaaggcgg cctgagccga
    10201 actcgcccgc acacaggcag acttgaaagg aggtgaaacc ctgtggttga ggaccggatc
    10261 actcagcgcg tcgttaccgt ggtcatggtc gtcatcgccg ccctggcgtt cgtcttctcc
    10321 ttcggcaacg tctggtcact ggccctgcgt ctcggcgtcc ccaccccgat tgcaccactg
    10381 atcgcgccca tggtggacct gtcggtggtc gggctcctgg tcgcgttgcg ctacctctcc
    10441 ctgcgcg
//
