LOCUS       NZ_WRXL01000004        11710 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Haloarcula salina strain YGHS18 scaffold4, whole genome shotgun
            sequence.
ACCESSION   NZ_WRXL01000004
VERSION     NZ_WRXL01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Haloarcula salina
  ORGANISM  Haloarcula salina
            Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
            Halobacteria; Halobacteriales; Haloarculaceae; Haloarcula.
REFERENCE   1  (bases 1 to 429703)
  AUTHORS   Zhang,W.J. and Cui,H.L.
  TITLE     Halomicroarcula salina sp. nov., isolated from a marine solar
            saltern
  JOURNAL   Int J Syst Evol Microbiol 65 (Pt 5), 1628-1633 (2015)
   PUBMED   25721722
REFERENCE   2  (bases 1 to 429703)
  AUTHORS   Zhang,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2019) Department of Biology, Anhui Normal
            University, No. 1 Beijing East Road, Wuhu, Anhui 241000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010119195.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 11:42:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,870
            CDSs (total)                      :: 3,820
            Genes (coding)                    :: 3,795
            CDSs (with protein)               :: 3,795
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 11 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 5 of 25
            Pseudo Genes (multiple problems)  :: 6 of 25
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:19:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 223297
            Orig. end                         :: 235007
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WRXL01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11710
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11710
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11710
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6710](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6710
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            77..424
                     /locus_tag="GM656_RS12530"
     CDS             77..424
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GM656_RS12530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_162414019.1"
                     /transl_table=11
                     /translation="MVVRRLLGLLVSGTNRNSNRKVRRVRRNAVEIVTRPFRTLYAIAF
                     MTLLLGVMYTLIYDSGAAAAVDAAEGDAVAMALAFLPPLPVLALIAVAMAILVPFSMLF
                     RGVSDENRYHR"
     gene            complement(443..1690)
                     /gene="glyA"
                     /locus_tag="GM656_RS12535"
     CDS             complement(443..1690)
                     /EC_number="2.1.2.1"
                     /GO_function="GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0070905 - serine binding [Evidence IEA]"
                     /GO_process="GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (59-375). E-value:
                     5.4e-12. Score: 37.4. Matches aSDomain:
                     nrpspksdomains_GM656_RS12535_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="glyA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008608059.1"
                     /locus_tag="GM656_RS12535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydroxymethyltransferase"
                     /protein_id="WP_162414020.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 3.5e-11, bitscore:
                     37.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTYETVRESDPAVADALEGERTRQNDTLAMIASENHVSEAVMEAQ
                     SSELTNKYAEGYPGERYYGGCEYADDVEEIAIDRAKELWGADHVNVQPHSGSQANMGVY
                     LAMLDAGDKILSLDLTHGGHLSHGHPANFAGQVYEVEQYEVDDETGYIDYEQLRETAEA
                     FEPDIIVSGYSAYPREIEWETIQEAADAVDAYHLADIAHITGLVAAGVHSSPVGIADFV
                     TGSTHKTIRAGRGGIIMCDEEYADDIDSAVFPGTQGGPLMHNVAGKAVGFGEALAPEFE
                     AYAQQTVDNARALGDRLQEHGLDLVSDGTDNHLVLIDLRPSHPDTTGKDVEAALEEAGI
                     VLNANTVPGETRSAFNPSGIRAGTPALTTRGFDEAACEEVADLIYEIVDAPHDDAVIAE
                     VSDRVDELTDEYTLYD"
     aSDomain        complement(566..1513)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GM656_RS12535_Aminotran_1_2.1"
                     /evalue="5.40E-12"
                     /label="GM656_RS12535_Aminotran_1_2.1"
                     /locus_tag="GM656_RS12535"
                     /protein_end="375"
                     /protein_start="59"
                     /score="37.4"
                     /tool="antismash"
                     /translation="ERYYGGCEYADDVEEIAIDRAKELWGADHVNVQPHSGSQANMGVY
                     LAMLDAGDKILSLDLTHGGHLSHGHPANFAGQVYEVEQYEVDDETGYIDYEQLRETAEA
                     FEPDIIVSGYSAYPREIEWETIQEAADAVDAYHLADIAHITGLVAAGVHSSPVGIADFV
                     TGSTHKTIRAGRGGIIMCDEEYADDIDSAVFPGTQGGPLMHNVAGKAVGFGEALAPEFE
                     AYAQQTVDNARALGDRLQEHGLDLVSDGTDNHLVLIDLRPSHPDTTGKDVEAALEEAGI
                     VLNANTVPGETRSAFNPSGIRAGTPALTTRGFDEA"
     gene            1860..3938
                     /locus_tag="GM656_RS12540"
     CDS             1860..3938
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518089.1"
                     /locus_tag="GM656_RS12540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_162414021.1"
                     /transl_table=11
                     /translation="MVAWGVSGVEVDGDADSTTEWDGSEVDPAALVRQLHRSVSVGTEQ
                     LQRPADECYRGTVTEIALPAVEPTLSRLDAEPYRGTLSELPEGTFLLTMQAPAAVADRA
                     SPFADADEIRIHLRFDGPAYVRSLGTTTVLAFDDWTAVTLGFVSVSSESRPQIQVPETP
                     AGLATAVTYMSSAFHTMKPSRSHPEFRGHPPLVATGEPVQIPEAVRECRPDTGIRLRVP
                     ERVADVLVTAPLAYYLGAEVTVESRDSALLTADGTDVSISLGERSSLQCGVAQLLRKVF
                     YLDCQVRHIDPDQNGDRITAALSLDADAVCSLPPAERLERYVDVSDDAVNSILPEWPLS
                     TYVEPRADRVRCLPFLLERLSLVYLPDGSELDQSDLLDKTLADSYLCRGSAERPSMIEP
                     TLREGQLHAWLAPGNPIDACKTTPEAYLNRYRFECRCDSEPTVAVVLNDEEMADEHTEV
                     TEIYRAADLPMDVRVNERLSVAELADAFERQTDFVHFIGHCDDVGLRCPDGNLPLSSLD
                     GTGTQTFFLNACGSYQQGLDLVEQGAVAGAVTFTDVLDKHAALVGTAFARLLCNGFSIQ
                     RALELARRRIMMCKDYAVVGDGTYSIIPCPDQPAVVWLSESESGFDLDCTVVSPERPGE
                     SYRLPFGDGAVLNGEQTTHTLERDELAAALSGTSLPVIYDGEFHWSDDLAARLQSGF"
     gene            4151..4969
                     /gene="tbsP"
                     /locus_tag="GM656_RS12545"
     CDS             4151..4969
                     /codon_start=1
                     /gene="tbsP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008309020.1"
                     /locus_tag="GM656_RS12545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transcriptional regulator TbsP"
                     /protein_id="WP_162414022.1"
                     /transl_table=11
                     /translation="MTSNLLQDDVGSMLGAVFESTTEPVYVVNPSRQSVAELVSTLDAD
                     ADAPEVRLLADERALKDVMDDFLVASTAADLIDEDRLEMRLLGEVPNHSVAVTPDAVYA
                     LVTINDAVGGLGTDDGDFVDTAYDYYESTWADADEYSLRTPPLNRVRSTLESDIGQETA
                     DDFDDVLSSLSTARGDGDGLDEVTISLLVAARNGELLYDISKWGEDVGLASKATFSRTK
                     TKLEDMNLIDTEKVPIDVGRPRLRLMLGDERLKDAEPDELATVAQSMLAA"
     gene            5001..6710
                     /locus_tag="GM656_RS12550"
     CDS             5001..6710
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004961155.1"
                     /locus_tag="GM656_RS12550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_162414023.1"
                     /sec_met_domain="YcaO (E-value: 1.6e-60, bitscore: 200.3,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTVEVVGSGPAVASLTEFLADIDVALTRSSTPTESPADLFVVVD
                     TVGATAFESASDRASDAGTPWVAVELGGVGGVPVTDAAISAFGPETGCFDCLRRRVEAT
                     VDGSESPTEAPSAATQRFAGSLTGRLLTQFFDGERALFGTVVELPHTERRFLPVPGCDC
                     GDSPAPRLDAGHQTAHDSEALSRAEIGLDDRVGIVGEVGEARSFPSPYYLSTLADTAGF
                     SDVTAPRQAAGVAVDWDTAFMKALGESYERYAAGVYCVADLPSGTVESQADAVDPSAFV
                     RAESGWDDERAIPWYPAEDLATGSERSLPAETVLYPPPSNAVRTATTTGLGLGNTVTEA
                     LVTGLYEVIERDAAMLSWYSTFDPLRVTVDDHDRFDTLRRRAQSEGLDVTPLLLTQDVD
                     VPVVTVALEGESWPRFALGTDADLDPGAAAVGALEEALQNWMELDGMGPDGAKEAEGAI
                     GHYAESPGDAAGMTDTETAVPLDGLGSDADLSGEAELDALCERATDAGLTPCGARLTTR
                     DLEQLGFEAVRVVCPSAQPLFFGESFFGGRAERVPSDMGFEPRLDRDHHPFP"
     gene            complement(6732..7880)
                     /locus_tag="GM656_RS12555"
     CDS             complement(6732..7880)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518092.1"
                     /locus_tag="GM656_RS12555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF63 family protein"
                     /protein_id="WP_162414024.1"
                     /transl_table=11
                     /translation="MATYERVVGDFDSGQLWLGSFVAVVLVGIGAVFAAPQLVWDRFLW
                     HYFWGPVYADAMAANCAVMSPVGPEPLYEGCAAAIQEGRLVAEPGYTIVSEVGYMLILV
                     YMLVGVYLLLERLEIAEDPKLYFAFVPFMLLGGALRVVEDATDRAVEAGVDPLVDYPLS
                     SLIISPVIYGTVFLLTLLVLVVCVKLDNDGVVDSYYRTTGVVGTTLVLVTLLYLTFVAV
                     TTDYSRLYPSVLITTVGIASVLSYGLYRLFDRYWPAVNDGTGTIGLLVIWGHAIDGVAN
                     VLLADWLDALSVPLTYYPKHPANAFIIETTEAIQPAALSAAIGTSWPFLVVKLAVASLV
                     VSLFNEEFMEDSPRYALLLLIAVTAVGLGPGTRDMLRATFAI"
     misc_feature    complement(6804..6806)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7972..8811)
                     /locus_tag="GM656_RS12560"
     CDS             complement(7972..8811)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1207: inositol monophosphatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011223369.1"
                     /locus_tag="GM656_RS12560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="inositol monophosphatase"
                     /protein_id="WP_162414025.1"
                     /transl_table=11
                     /translation="MTDAHHRAAVAERAARAGGVVAREGFRGSFSVESKANKNDLVTEL
                     DRDAQRQVVATVRAEFPTDAFLCEEPVTTLGAGENAEGATEVDDVPERGPAWIVDPIDG
                     TANYVRGMRLWATSVSAVVDGEPVASATYAPSQGDLYAVGPESATRDGTELAVSDRTDP
                     ETFAVAPVGWWDRDERGEFGSLCTAIAERFGDVRRLGSFQLTLAHVADGALEGLICTRP
                     MNPWDTVAGVQLVRRAGGTVTDLDGEPWSHDSAAMVASNGEAHDALVAAGNEALGGE"
     gene            complement(8884..9081)
                     /locus_tag="GM656_RS12565"
     CDS             complement(8884..9081)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518094.1"
                     /locus_tag="GM656_RS12565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_162414026.1"
                     /transl_table=11
                     /translation="MATDQSEADLRVPPLENTAFFSIENDDGYVRIEQSLGNQVLYTPS
                     EARDIAEEILDAVDGAVETD"
     gene            complement(9156..9791)
                     /locus_tag="GM656_RS12570"
                     /pseudo=""
     CDS             complement(9156..9791)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011224772.1"
                     /locus_tag="GM656_RS12570"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS6 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDLDFVERKRTPESAMALGIQSYVAGLSLSNTDELLEALGVQRSR
                     KAIHDWVQKANLQPESGKSPNQIALDETVIRINDQQFWLYAVLRMA"
     gene            complement(10182..10857)
                     /locus_tag="GM656_RS12575"
                     /pseudo=""
     CDS             complement(10182..10857)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011224772.1"
                     /locus_tag="GM656_RS12575"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS6 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAEITRLNGHRDWVDLDFVERERTPGWAVALGIQSHVAGLSLRNT
                     VELLDCLGVQRSRKAIHDWVQKADLQPKSGKSPNQVALDETVIWINDQQFWLYAVLRMA
                     MPF"
     misc_feature    complement(10648..10650)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(10714..10716)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 acgccgcacg cctcgggcga gcgcgtcggt ggtactcggg ggatacgctc atgctgtcag
       61 ccatccattg tgtcacatgg ttgtacgacg gctcctgggt ctgctcgtca gcggaacgaa
      121 ccgaaactcg aaccgaaaag tgaggcgagt ccggcgcaac gccgtcgaga tcgtcacccg
      181 tccgtttcgg acgctctacg cgatcgcgtt catgaccctg ctgctcggcg tcatgtatac
      241 gctcatctac gacagcggag cggccgctgc cgtcgacgcc gcggaggggg acgcggtcgc
      301 gatggcactg gcgttcctgc cgccgctccc cgtcctcgcg ctcatcgccg tcgcgatggc
      361 gatcctggtc ccgttctcga tgctgttcag gggcgtcagc gacgaaaacc ggtatcaccg
      421 gtaacaggac gttcggaacc gctcagtcgt acagcgtgta ctcgtcggtc agttcgtcga
      481 cgcggtcgct gacctcggcg atgaccgcgt cgtcgtgggg cgcgtcgacg atctcgtaga
      541 tgaggtccgc gacctcctcg caggcggcct cgtcgaagcc gcgggtcgtg agcgcgggcg
      601 tgccggcgcg gatgcccgag gggttgaacg ccgagcgcgt ctcgccgggg acggtgttcg
      661 cgttgagcac gataccggcc tcttcgagcg ccgcctcgac gtccttgccc gtggtgtctg
      721 ggtgggaggg gcggaggtcg atgaggacga ggtggttgtc cgtgccgtcc gagacgaggt
      781 cgaggccgtg ttcctgcaga cggtcgccga gcgcgcgggc gttgtcgacg gtctgctgtg
      841 cgtacgcctc aaactcgggg gcgagcgcct cgccgaagcc gacggccttg ccggcgacgt
      901 tgtgcatcag gggaccgccc tgggtgccgg ggaagacggc ggagtcgatg tcgtcggcgt
      961 actcctcgtc gcacatgatg atgccgccgc ggccggcacg gatggtcttg tgcgtcgaac
     1021 cggtgacgaa atcggcgatg ccgaccggcg aggagtggac gccggcggcg acgaggccgg
     1081 tgatgtgggc gatgtcggcg aggtgatacg cgtcgacggc gtcggcggcc tcctgaatgg
     1141 tctcccactc gatctcgcgc gggtacgcgg agtagcccga gacgatgatg tcgggctcga
     1201 acgcttcggc cgtctcacgc aactgttcgt agtcgatgta gcccgtctcg tcgtcgacct
     1261 cgtactgctc gacctcgtac acctggccgg cgaagttcgc cgggtggccg tggctgagat
     1321 ggccgccgtg ggtcaggtcc agcgagagta tcttgtcacc ggcatcgagc atggcgaggt
     1381 agacgcccat gttggcctgc gagccggagt gtggttggac gttgacgtgg tcggcgcccc
     1441 acagctcctt cgcgcggtcg atagcgatct cctcgacgtc gtcggcgtac tcacagccgc
     1501 cgtagtagcg ctcgccggga tagccctcgg cgtacttgtt cgtcagttcg gagctctggg
     1561 cctccatgac ggcctcgctg acgtggttct cactggcgat catcgccagc gtgtcgttct
     1621 gtcgtgtccg ctcgccttcg agcgcatcgg ctactgccgg gtcggactcc cgtacagtct
     1681 cgtaggtcat gtgtgcgcct cggaacccgg cggacaataa tctacccctc tggtgcgggc
     1741 gtgcggctga ctggcacgta acggatcgga gcttcggcga agtcgttagt aaccggcgtt
     1801 cacatgtcaa ttgatggtac ccatatgtat ttattgaata aggacacaca tgtcagtgta
     1861 tggtcgcctg gggggtgtca ggcgtggagg tcgacgggga cgcggattcg acgacggagt
     1921 gggacggaag cgaagtcgac ccggccgcgc tggtacgaca gttgcacagg agcgtgtccg
     1981 tcgggaccga acagctccag cgcccggcgg acgagtgtta ccgtggaacg gtcaccgaga
     2041 tcgcgctgcc ggcggtggag ccgacgctgt cccggctgga cgcggagccg tatcggggca
     2101 cgctgtcaga actccccgaa gggacgtttc tcctgacgat gcaggcgccc gcggcggtgg
     2161 ccgatcgagc gtcgccgttc gccgacgcag acgagattcg catccatctc cgcttcgacg
     2221 gtcccgccta cgtgcgttcg ctgggtacca ccacggttct cgccttcgac gactggacgg
     2281 ccgtcacgct cgggttcgtg tcggtttcca gcgagtctcg gccgcagata caggtccccg
     2341 agacgcccgc tggactcgcg acggcggtta cctacatgag cagcgcgttc cacacgatga
     2401 agccgtcgcg atcacatccc gagttccggg ggcatccgcc gctggtcgcg acgggagagc
     2461 cggtacagat ccccgaggcc gttcgggagt gtcgacccga caccggaatc cgtctccgcg
     2521 tcccggagcg cgtcgccgac gtgctcgtca ccgccccgct agcgtactac ctcggtgcgg
     2581 aggtcaccgt cgaatcgcgt gattcggcgc tgctgaccgc cgacgggacg gacgtctcga
     2641 tctcgctcgg cgagcgttcc tcgctccagt gtggcgtcgc gcaactgctc cggaaggtgt
     2701 tctacctgga ctgccaagtc aggcacatcg acccggacca gaacggagac cggatcacag
     2761 ccgccctctc gctcgatgcg gacgcggtct gctcgctgcc tcccgcggag cgactcgaac
     2821 gctacgtcga cgtctcggac gacgcggtaa acagtatcct ccccgagtgg ccgctctcga
     2881 cctacgtcga gccgcgggcg gatcgagtac gatgtcttcc ctttctgctg gaacgtctca
     2941 gtctcgtcta cctacccgac ggatccgagc tcgatcagag cgacctcctc gacaagaccc
     3001 tcgcggactc gtacctgtgt cgcggctcgg ccgagcgccc gtccatgatc gaaccgaccc
     3061 ttcgagaggg gcagctacac gcctggctcg cgcccgggaa cccgatcgac gcctgcaaga
     3121 cgacaccaga agcctacctg aaccggtacc ggttcgagtg tcggtgcgat agcgagccga
     3181 ccgtcgcggt ggttctcaac gacgaggaga tggcggacga acacaccgaa gtgacggaga
     3241 tctaccgggc cgccgacctc ccgatggacg tccgggtgaa cgagcggcta tcggtcgccg
     3301 aactggccga cgcgttcgaa cgtcagaccg acttcgtcca cttcatcggc cactgcgacg
     3361 acgtcggact gcgctgcccg gacggcaacc tcccgctgtc gtcgctcgac ggcacgggta
     3421 cccagacctt cttcctgaac gcctgtggct cctatcagca gggactcgat ctcgtcgagc
     3481 agggcgccgt cgccggcgcg gtgacgttca cagacgtgct cgacaaacac gccgcgctgg
     3541 tcgggaccgc gttcgcccgc ttgctgtgca acggcttcag catccagcgg gcgctcgaac
     3601 tcgctcgccg ccggatcatg atgtgcaaag actacgccgt cgtcggggac gggacgtact
     3661 cgatcatccc gtgcccggac cagcccgcgg tggtctggct gtcggagtcg gagtcgggct
     3721 tcgacctcga ctgcaccgtc gtctcgcccg agcgaccggg cgagagctac cggctaccgt
     3781 tcggcgacgg cgccgtgctg aacggtgagc agacgaccca cacgctcgaa cgggacgaac
     3841 tggccgcggc gctgtccggg acgtcgctgc cggtcatcta cgacggcgag ttccactggt
     3901 ccgacgacct ggccgcgcga cttcagagcg ggttctgaac ggcccggcgg gcggcttcga
     3961 atccgttcaa tgaccacagc gaccgccagc atagcgcatt ttccagccct gatatcgagt
     4021 tctgcttatt cgtccgtgtc gatattttta tcgcgaatat tctgtgggtg ttgagccgtg
     4081 agacgagaaa tcaacacata tcggccggta aaattaaata tattaatgcg ctatatactg
     4141 gcagaagaaa atgacttcaa atctcctgca ggacgacgtc ggttccatgc tcggggcagt
     4201 gttcgagtcg acgaccgaac cggtgtacgt cgtcaatccg tcccgtcaga gcgtcgccga
     4261 actcgtctcg acgctcgacg cggacgccga cgctccggag gtgcgactgc tcgccgacga
     4321 gcgcgcactg aaagacgtga tggacgactt cctcgtcgcc agcaccgcgg ccgacctcat
     4381 cgacgaagac aggctcgaga tgcgactgct cggtgaggtg ccgaaccact ccgtcgccgt
     4441 gacaccggac gcggtgtacg cgctcgtcac catcaacgac gcggtcggcg gtctcggaac
     4501 cgacgacggc gacttcgtcg acaccgccta cgactactac gaatcgacct gggcggacgc
     4561 cgacgagtac tcgctccgga cgccgccgct caaccgcgtc cgcagcacgc tcgaatccga
     4621 catcggtcag gagaccgccg acgacttcga cgacgtgctg agttcgctgt cgacggcccg
     4681 cggcgacggt gacggcctgg acgaggtcac catcagtctg ctggtcgcgg cccgcaacgg
     4741 cgaactgctc tacgacatca gcaagtgggg cgaagacgtc ggcctcgcca gcaaggcgac
     4801 cttctcccgg acgaagacca agctcgagga catgaacctc atcgacaccg agaaggttcc
     4861 catcgacgtc ggccgaccgc ggctccgcct gatgctcggc gacgagcgcc tcaaggacgc
     4921 cgaaccggac gagcttgcga cagtcgccca gtcgatgctc gccgcatagg tttttccccg
     4981 ttcacgccga cgccttcggc atgccaactg tcgaagtcgt cgggagtggg ccggcggtcg
     5041 cctcactcac cgagtttctc gccgatatcg acgtcgcact gacgcggagt tcgacaccga
     5101 ccgaatcgcc tgcagacctg ttcgtcgtcg tcgataccgt cggcgcgacg gcgttcgagt
     5161 ccgcgtccga ccgagccagc gacgccggga cgccctgggt ggccgtcgaa ctgggcggcg
     5221 tcggcggcgt tcccgtgacc gacgccgcga tcagcgcgtt cgggccggag accggctgtt
     5281 tcgactgcct tcgccggcgg gtggaagcga cggtcgacgg gtccgagtcg ccgaccgaag
     5341 ccccttcggc cgctacgcag cgcttcgccg gttcgctgac cggacggctc ctgacgcagt
     5401 ttttcgacgg cgagcgggcg ctgttcggga ccgtggtcga actcccgcac acggagcggc
     5461 ggttcctccc cgtcccgggg tgtgactgcg gtgactcgcc ggctccccga ctcgacgcgg
     5521 gacaccagac ggcacacgac agcgaggcgc tctcacgggc cgaaatcggc ctcgacgacc
     5581 gggtgggaat cgtgggggag gtcggggaag cgcggtcgtt cccgtccccg tactacctct
     5641 cgacgctggc cgacacggcg ggcttcagcg acgtgaccgc accgagacag gccgccggcg
     5701 tcgcggtgga ctgggacacc gcgttcatga aggcgctggg cgagagctac gagcgctacg
     5761 ccgccggcgt ctactgcgtc gccgacctcc cgagcgggac ggtcgagtcc caggccgacg
     5821 ccgtcgatcc gtcggcgttc gtcagagcgg aatccgggtg ggacgacgag agggcgattc
     5881 cgtggtatcc ggccgaggac ctcgcgaccg gttcggagcg gtcgttgccc gccgagacgg
     5941 tcctgtaccc gccgccgtcg aacgcagtca ggacggcgac gacgaccggc ctgggcctcg
     6001 gcaacaccgt cacggaggcg ctggtgactg gcctgtacga ggtcatcgag cgcgacgccg
     6061 cgatgctgtc gtggtactcc acgttcgacc cgctccgggt gaccgtcgac gaccacgacc
     6121 gcttcgacac gctgcgacgg cgcgcgcagt cggaggggct ggacgtgacg ccgctgttgt
     6181 tgacccagga cgtggacgtg cccgtcgtca ccgtcgcgct ggaaggggag tcgtggccgc
     6241 ggttcgcgct ggggaccgac gccgacctcg atccgggggc ggccgccgtc ggcgcgctcg
     6301 aagaggccct ccagaactgg atggaactcg acggcatggg acccgacggc gcgaaagagg
     6361 cggagggcgc gatcggccat tacgccgagt cgccgggcga cgcggccggg atgaccgaca
     6421 ccgagaccgc ggtgccgctc gacggcctcg gctccgacgc ggacctgtcc ggcgaggcgg
     6481 aactcgacgc gctctgcgag cgggcgacag atgccggcct gacgccctgc ggcgcccggc
     6541 tcacgacgcg ggacctcgaa cagctcggct tcgaggccgt tcgcgtggtc tgtccgtcgg
     6601 cacagccgct gttcttcggc gagtcgttct tcggcgggcg cgccgagcgc gtgccgagcg
     6661 acatggggtt cgagccgcgc ctcgacaggg accatcaccc gttcccgtag aacagtgaga
     6721 ccgcccgacg ctcagatcgc gaacgtcgcg cgcagcatgt cgcgcgtccc cggaccgagg
     6781 ccgaccgcgg tcacggcgat gagtaacagg agcgcgtacc gcgggctgtc ctccatgaac
     6841 tcctcgttga acagcgagac gacgagcgag gcgacggcca gtttgaccac gaggaagggc
     6901 caggacgtgc cgatggcagc cgagagcgcg gccggctgaa tcgcctccgt cgtctcgatg
     6961 atgaacgcgt tcgcggggtg tttcgggtag taggtcagcg ggacgctgag cgcgtcgagc
     7021 cagtccgcga gcaggacgtt cgcgaccccg tcgatggcgt gcccccagat gacgagcaga
     7081 ccgatggtgc cggtcccgtc gttgaccgcg ggccagtacc ggtcaaacag ccggtagagg
     7141 ccgtacgaca gtactgaggc gatcccgacc gtcgtaatga ggacgctggg atagagccgc
     7201 gagtagtcgg tggtgaccgc cacgaacgtc aggtacagca gggtgacgag gacgagggtc
     7261 gtaccgacca ccccggtcgt ccggtagtag ctgtcgacga cgccgtcgtt atcgagcttc
     7321 acgcagacga cgagcacgag cagcgtgagc aggaacaccg tcccgtagat gacggggctg
     7381 atgatgagcg aggacagcgg gtagtcgacg agcggatcga ccccggcttc gaccgcgcgg
     7441 tcggtggcgt cctcaacgac gcgtagcgca ccgccgagca gcatgaaggg gacgaacgcg
     7501 aagtagagct tcgggtcctc cgcgatctcc agtcgctcca ggaggaggta gacgccgacg
     7561 agcatgtaga cgagaatgag catgtagccc acctcggaga cgatggtgta gccgggttcg
     7621 gcgaccagtc gtccttcctg aatcgcggcc gcacagccct cgtagagcgg ttccgggcca
     7681 accggggaca tgacggcgca gttcgcggcc atcgcgtccg cgtacaccgg cccccagaag
     7741 tagtgccaga ggaaccggtc ccagaccaac tgtggggccg cgaagaccgc cccgataccg
     7801 acgagaacga ccgcgacgaa cgacccgagc cagagctgcc cggagtcgaa atcaccgacg
     7861 actcgctcgt acgttgccat ggccgtgtga ccggtggctg gcggttttag cgttccggtc
     7921 gcgtgcgcgt gacggacgaa atcggccggt cagcgatttc gccgagacgg ctcattcgcc
     7981 gccgagggcc tcgttgccgg ccgcgacgag cgcgtcgtgc gcctcgccgt tggaggcgac
     8041 catggcggca ctgtcgtggc tccagggctc gccgtcgaga tccgtcaccg tcccgccggc
     8101 ccgtcggacc agttgcacgc cggcgacggt gtcccagggg ttcatcggcc gggtgcagat
     8161 cagcccctcg agcgcgccgt cggcgacgtg tgcgagcgtc aactggaacg acccgaggcg
     8221 gcgaacgtcg ccgaagcgct cggcgatggc ggtacagaga ctgccgaact cgccccgctc
     8281 gtcgcggtcc caccagccga cgggcgccac cgcaaacgtc tcggggtcgg tccggtcgct
     8341 cacggcgagt tcggtcccgt cacgagtggc gctctcgggg ccgacggcgt agaggtcgcc
     8401 ctgtgacggc gcgtacgtcg ccgacgccac gggctcgccg tcgacgacgg cgctgacgct
     8461 cgtcgcccag aggcgcattc cacggacgta gttcgcggtc ccgtcgatgg ggtcgacgat
     8521 ccaggccggt ccccgctcgg gcacgtcgtc cacctcggtc gctccctcgg cgttttcgcc
     8581 cgctcccagc gtcgtcacgg gctcttcgca gaggaacgca tcggtgggaa actccgcgcg
     8641 gacggtggcg acgacctgcc gctgagcgtc gcggtccagt tccgtgacga gatcgttctt
     8701 gttggccttc gactcgacgg agaacgatcc ccgaaacccc tcgcgtgcga cgacgccgcc
     8761 ggcgcgggcc gcccgctcgg cgaccgccgc ccggtggtgc gcatcagtca tatcagccgc
     8821 tggtcgtccc tcgttcaaac cacctcggct tccagcaaaa cggcccgcgc gcggagagag
     8881 cggtcagtcc gtctcgacgg ccccgtcgac ggcgtcgaga atctcctcgg cgatgtctcg
     8941 cgcctcgctt ggcgtgtaca ggacctgatt ccccagggat tgctcgatac ggacgtaccc
     9001 gtcgtcgttc tcgatggaga agaacgccgt gttctcgagc ggtggcacgc gcaggtccgc
     9061 ctcagactgg tcggtcgcca tgtcaatata tatcatacgg agcgaaataa atctacgccc
     9121 cggacgagag gtagtgttta gtttggagca ttgtgtcagc gtgcgaaact ctgcaaccag
     9181 ttttcggctg tttccggttg gacgtgactg aagcaatttg aaaatgacga ggtgcgacgt
     9241 ttcagctctc gaaaaattcg ctcgacagcg ttccgatttc cgtggcgaca catctgaaat
     9301 cggaggccag ctcgttgcag cgcagtttgg aggtgttgag cgccatcaac gagaaacacg
     9361 gcagtttcga tatcgtgttt ttggcgaagt tctcgcagga aaattccgtg agagtggtcg
     9421 tagtcgtcgc aaacagccgg acgtgaagca gttcattcga ctgcggatcg gcggcgttcg
     9481 caagagcaca gctcttgcgc agcccatcag aatggctatg ccattctgag gacggcgtac
     9541 agccagaact gctggtcgtt gattcgaatc actgtttcgt caagcgcgat ctgattcgga
     9601 gatttacccg attcgggctg tagattggct ttttgcaccc aatcgtgaat cgcttttcga
     9661 ctgcgttgga cacccagcgc ttcgagcaat tcgtcggtat tcgacagtga caaccccgca
     9721 acgtacgatt gaatacccag cgccatcgcc gactcgggtg tccgcttgcg ctccacaaaa
     9781 tccaaatcaa ttcagtcgct acttccgctg aggcggctga tttctgccat agaccagcaa
     9841 gaaatccgtc cgcatcactt ttcacgctta actaaacacg accctcgaaa atgcctcgat
     9901 atagttatga tcgaaacaca aggagatacg ttccggagac cagtgtaaca aaaccgccaa
     9961 ccatcgttga aaaagctact gcttgggctg ccacatcctg cgtacactgc tgctctaaac
    10021 ccgtgccata ggcacacctg ccggtcggca tactgaaacc gataagtaag attattgcac
    10081 caacaagaat aatcaaatat atttgccaat ttttcatttc tattagacaa cactcatatg
    10141 ataggtttta atcttggtag tgtttagttt ggagcattgt gtcagcgtgc gaaactctgc
    10201 agccaatttt cggctgtttc gggttcaacg tggctgaagc agtttgaaaa cgacgaggtt
    10261 cggcgcttca gttctcgaaa aattcgctcg atggcgttcc gatttccgtg gcgacgcatc
    10321 tgaaatcgga ggccaactcg ggaaagtgca gtttggaggt gttgtgcacc gtcgacaaga
    10381 aattcagagg tttcgacatc gtgttcctgc cgcagttctc gcaggaaaat ttcggtgaga
    10441 gcggtcgtgg tcgttgaata caaccggacg tgaagcagtt cgttcgattg gggatcggcg
    10501 gcgttcgcaa gagcacagct cttgcgcagc ccatcagaat ggcatagcca ttctgaggac
    10561 ggcgtacagc cagaattgct ggtcgttgat ccaaatcact gtttcgtcga gcgcgacctg
    10621 attcggagat ttccccgatt taggctgtaa atcagctttt tgtacccaat catggatggc
    10681 tttccgactc cgttggacac ccaaacagtc gagtaattca acggtattcc tcagcgagag
    10741 tcccgcaacg tgtgattgaa tacccaacgc caccgcccac ccgggtgtcc gctcgcgctc
    10801 cacaaaatcc aaatcaaccc agtctctatg accgttgagg cgggtgattt ctgccatgaa
    10861 ccagcaagaa attctcccgc ctcacttttc acgcttaact aaacaccgcc accgcttccc
    10921 ggcagaccgt tatggcttct gagaattggg ccgcaacaat ttaccaataa tcgctatgag
    10981 tgcgtgtatg atgtctctcc ggggaatttt ccggtcggga aaggaccttt cgatcatcgt
    11041 ggttctcggg ctcgtattcg tactcgggct gaccgtcaaa acgctgttcg agttgagtaa
    11101 acggaagttt ccgcgcgccc acgcgatggg aagtgaagtc agagactggc ttcgccgtcc
    11161 gatatgacta cgactgccgt tccgctctgt cccagcgcgc ccgcgttcta tcgtcgcagg
    11221 aacccacgag acgggtcagc gagatcctct gtgaatttgt ctgaaatcta tctattgtct
    11281 cggttgggca ctattgataa attatctgtt agttttgatg gcgcggtcaa atatagtcat
    11341 tgcatgatat aaaaagcagt tgtctaattc gctcaccgac cgatccgatt tttcactttt
    11401 ccacctcctc attgtctttc aaggtattct tcgtatcttg tcgatctagc cacctagcgg
    11461 acacgttagc ccgaccttaa tgccatgatt aattgcacac atttttaccc gacagcttta
    11521 tgagaaattg gattgacatg acgagtaacg tgtgagatcg gtaactcatc gagtcaccgt
    11581 tggattgcca cagcacctat gactgacacc cataccgccc gaacaggtga gagactgacg
    11641 agtgaccgac caggggtccc acggagcgga gagagaaccc cactggaagg ggaatgacga
    11701 taaacgcaaa
//
