LOCUS       NZ_NKBC01000006        10345 bp    DNA     linear   CON 17-MAR-2024
DEFINITION  Bacillus paralicheniformis strain 9-1 9-1_R1_(paired)_contig_6,
            whole genome shotgun sequence.
ACCESSION   NZ_NKBC01000006
VERSION     NZ_NKBC01000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus paralicheniformis
  ORGANISM  Bacillus paralicheniformis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 683818)
  AUTHORS   Cui,Y.
  TITLE     Improper usage of probiotics constitutes a potential risk of public
            health
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 683818)
  AUTHORS   Cui,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2017) Beijing Advanced Innovation Center for Food
            Nutrition and Human Health, College of Veterinary Medicine, China
            Agricultural University, Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 9.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009935535.1-RS_2024_03_17
            Annotation Date                   :: 03/17/2024 02:24:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,539
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,361
            CDSs (with protein)               :: 4,361
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1 (5S, 16S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 49 of 100
            Pseudo Genes (incomplete)         :: 64 of 100
            Pseudo Genes (internal stop)      :: 15 of 100
            Pseudo Genes (multiple problems)  :: 21 of 100
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:47:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 128038
            Orig. end                         :: 138383
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NKBC01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10345
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10345
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10345
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5345](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5345)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            44..394
                     /locus_tag="CE036_RS04410"
     CDS             44..394
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390372.1"
                     /locus_tag="CE036_RS04410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_011198121.1"
                     /transl_table=11
                     /translation="MEIAIIALLVVSIALIAFSYFQREPIKEVEQELETLQLSAMQEIY
                     KLKKKMTVLEEELLDSNVVVRRPNAGISQHIAKQILSKYQNGMSVDAIAKAEHVSVEDV
                     KAIIKDNERVLV"
     gene            391..870
                     /locus_tag="CE036_RS04415"
     CDS             391..870
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183520.1"
                     /locus_tag="CE036_RS04415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="endolytic transglycosylase MltG"
                     /protein_id="WP_020452195.1"
                     /transl_table=11
                     /translation="MTRQSVQAFAGGMILATAVLAGAFYLTGNGKAEASKDAQNISETD
                     VKTYLKDNQQVSLKREEYQQLLHYKETALKQDASSQNKKEKEAEKKDSKYKLEIKSGMS
                     TAEIADLLAKGKVISSADDFKDYVKKTGNESKIRAGKYELKRGDSLKNIVKTLSK"
     gene            complement(927..>1179)
                     /locus_tag="CE036_RS04420"
                     /pseudo=""
     CDS             complement(927..>1179)
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611387.1"
                     /locus_tag="CE036_RS04420"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="CLLCDQG"
     misc_feature    1544..1555
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.02"
                     /tool="antismash"
     gene            complement(1693..1989)
                     /locus_tag="CE036_RS04430"
     CDS             complement(1693..1989)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179490.1"
                     /locus_tag="CE036_RS04430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyltransferase"
                     /protein_id="WP_096748227.1"
                     /transl_table=11
                     /translation="MFFNKKQIDKLLDDHTGEEQKETKSKVNTSFIIVFYFSILLSICT
                     VFFDYRYFLIALFGPTILVTFISPPFQTKKGIYRIFSAIYLILLITVTFFWLN"
     gene            complement(1979..2533)
                     /locus_tag="CE036_RS04435"
     CDS             complement(1979..2533)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /locus_tag="CE036_RS04435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage II sporulation protein M"
                     /protein_id="WP_020452197.1"
                     /transl_table=11
                     /translation="MKELIKRNKRILYIASSFYIVGLVLGVIFNNILPSLSQDNVQNIT
                     VSGYFTHNFAANLFLIFSMFTFGILTSILLLLNGFLVGLSSLHSITDHGNDVLFILTAL
                     VPHGIFEIPAMILSGALGFKLLDYVYMKIRGIYFSKKQFLQDFLYFVIILFLLTLLAAV
                     TEALITPYLLNKLTLGGSYVF"
     gene            complement(2530..3237)
                     /locus_tag="CE036_RS04440"
     CDS             complement(2530..3237)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="CE036_RS04440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_020452198.1"
                     /transl_table=11
                     /translation="MNIENVSFKYKDKLILESINLKIEKGKVYGLLGPNGAGKSTLLKC
                     ILGIVKPYEGKITVKGETLESERSSYLKYIGYVPDAPFIYHYLTPREYLEFVADLQEVP
                     YEIKNDRIDLLLSQFKLKDNENDMISTFSFGMKHKLSVAGAIVHEPEILILDEPLGAFD
                     PPTTKMMKSFLKRYAEQGNTVIMSTHLVNIAQQLCDKVGILINGTLVKEYNHLTDIKDF
                     EDLIIRDMDTILK"
     gene            complement(3234..4928)
                     /locus_tag="CE036_RS04445"
     CDS             complement(3234..4928)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CE036_RS04445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_020452199.1"
                     /transl_table=11
                     /translation="MLNKRVLIANFKLTYRRTLNRLIYFFPSSENLLKSNKILFVFAPF
                     FLVYIFILYKASQTLLTGVPEHILTLGTNFIILFFVLNILKSSTDIIHRYVYPEDFLLL
                     HTLKTDPKKILLSRFFTTIFLNILFKLTVVLFPILFFINFSTHDRIYLLLSFPSLLIFI
                     VGLTSLYSSLTYIVYSKFLHNILNSRLKSLLSLIGIACISAISTTFLGWIGLNYFYQND
                     LLEAIGNFIYLFKLDTILTTNWLPTNWFLLSTSDIHLFFLCSIGYIALGCLFAFITFHL
                     ILKVLIIKEAVPNNLLPKLKPIKLPFLSFLQKTLPRQNIAILKKDLTALLRANFTIKKR
                     LVLSINMIAVELGVLIGLSLNQKWFDFSDIFVIYPILFAVTYNTSLLGEGLLGVTSADA
                     ERENLYVYKSSGASIFKLIAGKSLLHLSVILILMNTLFLFILLLFNLDIMTQLILFIMI
                     NSISLIIGAGQIIGTFLYPRLDWENFEDIGSSVKASFFEHTILGVLLAFYLQLYGITGV
                     LVTFNIINRGVFFLIIVTGTILFVIGIGVFYYFWIKRIGTKNWEMKT"
     gene            complement(5001..5345)
                     /locus_tag="CE036_RS04450"
     CDS             complement(5001..5345)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03651.1"
                     /locus_tag="CE036_RS04450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_020452200.1"
                     /sec_met_domain="TIGR03651 (E-value: 2.4e-10, bitscore:
                     36.0, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNSNLFKIASITGVVSLFAIATFLMGSSEGIAGLASIDFSSGHQ
                     FALDLATNLGISKKTAYVAIGVIMTTGDVLTILSLLAVVLGGTGLITAAMVATAKKLAK
                     KYGKKYAAEW"
     gene            complement(5370..6023)
                     /locus_tag="CE036_RS04455"
     CDS             complement(5370..6023)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /locus_tag="CE036_RS04455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YIP1 family protein"
                     /protein_id="WP_020452201.1"
                     /transl_table=11
                     /translation="MDNKQNFITLVHHFIFSPKDFLSIKYSSDKNYRHSWFVFLAVLFL
                     FLGFYLAIFPTLSTLINNSFQDNGIDYTTDMNTLIMIITLVLGIIQFTSMVLVSFFIIH
                     IIGILIGGTATAKEFFPSFIYSIIPIGIKFTILIIFSFFLGSDFAFSVIYPNSQQPLDI
                     ILNIIIDPFFWWSTILGCIALKVIHQISYKKTIIIISITMSLGIISLFSTSLFL"
     gene            complement(<6214..6450)
                     /locus_tag="CE036_RS04460"
                     /pseudo=""
     CDS             complement(<6214..6450)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611387.1"
                     /locus_tag="CE036_RS04460"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGTRVSSG"
     misc_feature    complement(6530..6544)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 21.31"
                     /tool="antismash"
     misc_feature    6531..6545
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 21.22"
                     /tool="antismash"
     misc_feature    complement(6538..6547)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 17.07"
                     /tool="antismash"
     gene            complement(6621..7400)
                     /gene="pstB"
                     /locus_tag="CE036_RS04465"
     CDS             complement(6621..7400)
                     /EC_number="7.3.2.1"
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstB"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390374.1"
                     /locus_tag="CE036_RS04465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="WP_023855209.1"
                     /transl_table=11
                     /translation="MSAVTAVKPKDVYQINDLNLWYGEHHALKNINLTIPEYEITAIIG
                     PSGCGKSTFIKTLNLMINMVPNVKMTGEISYNGTNVLNSKVDLVELRKKVGMVFQKGNP
                     FPQSIFDNVAYGPRIHGLKNKKELNERVEKALVDVALWDEVKDRLHSQALGLSGGQQQR
                     LCIARALATNPDVLLMDEPTSALDPVSTLKIEELMLKLKEKYTIAIVTHNMQQASRISD
                     QTAFFLMGELIECNDTVKMFSSPDDYRTKDYITGRFG"
     gene            complement(7413..8240)
                     /gene="pstB"
                     /locus_tag="CE036_RS04470"
     CDS             complement(7413..8240)
                     /EC_number="7.3.2.1"
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstB"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016885932.1"
                     /locus_tag="CE036_RS04470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="WP_025811150.1"
                     /transl_table=11
                     /translation="MVQVQMPVLETVRDTIGIAPFPEAKEHILEVKNLNIYYGEKRAVN
                     DISMHVDKHAVTALIGPSGCGKSTFLRSINRMNDLIPSARTSGEIRYEGLNILDSNINV
                     VNLRREIGMVFQKPNPFPKSIYSNITHALKYAGERRKSVLDEIVEESLRKAALWDEVKD
                     RLHESAFSLSGGQQQRLCIARTLAMKPEVLLLDEPASALDPISNAKIEELIVGLKEEYS
                     IIIVTHNMQQASRISDQTAFFLNGDLIEYDRTEKIFMNPSEKKTEDYINGRFG"
     gene            complement(8260..9147)
                     /gene="pstA"
                     /locus_tag="CE036_RS04475"
     CDS             complement(8260..9147)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstA"
                     /gene_functions="transport (smcogs) SMCOG1214:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183530.1"
                     /locus_tag="CE036_RS04475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="WP_023855211.1"
                     /transl_table=11
                     /translation="MNSKVTDRLATGVFGLIAAVIAAILVGLFTYIIINGVSHISLSFL
                     TSRSSALSSGGGIRDQLFNSFYILALTMLITVPLGVGGGIYMAEYAPANKLTDFIRVCI
                     EVLSSLPSIVMGMFGMLMFVNLTGWGYSIIGGALALTIFNLPVMVRVTEDAIRSVPKEQ
                     KEASLALGVSKWHTIKTVLIPGAVPSIITGAILASGRVFGEAAALLFTAGLTTPRLNFA
                     DWNPFSETSPLNIFRPAETLAVHIWNVNTQGIIPDAEAIANGASAVLVLSVLIFNLAAR
                     WLGSFIHKKLTANK"
     gene            complement(9147..10076)
                     /gene="pstC"
                     /locus_tag="CE036_RS04480"
     CDS             complement(9147..10076)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstC"
                     /gene_functions="transport (smcogs) SMCOG1214:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612605.1"
                     /locus_tag="CE036_RS04480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="WP_003183532.1"
                     /transl_table=11
                     /translation="MVQKIEKLSAKERLISSKHNRKMDEIRGRILVASCAFIMMAAAIS
                     ITIFLGNKGLQSFLANGVSPIEFLTSLDWNPTNENPEYGALPFILGSFAVTLLASLIAA
                     PLGIGGAIFMTEIAPSWGRKVLQPVVELLVGIPSVVYGFIGLTVLVPFISQFKSSGSGH
                     SLLAGTIVLAVMILPTVTSISTDALMSLPKNLREGSYALGATRWQTIRKVLIPAALPSL
                     LTAVVLGMARAFGEALAVQMVIGNSRNLFESLLDPAGTLTTIITLNMGHTTYGSVENNT
                     LWSMGLVLLIMSFLFILLIRYLSSRRKL"
ORIGIN
        1 ggtataaaaa agaaggagaa aattagaatt gaaggtgatg aacgtggaaa ttgctatcat
       61 tgcgctgctc gttgtcagca ttgcgctgat tgcattctca tattttcaaa gagaaccgat
      121 caaggaagtt gaacaggagt tggaaaccct tcagctgtcc gccatgcagg aaatctataa
      181 attgaaaaag aagatgaccg tgcttgagga agagctcctc gattcaaatg tggtcgtccg
      241 cagaccaaat gccggcatca gccagcatat tgcgaagcag attctttcaa aataccaaaa
      301 cggcatgtct gtagacgcca tcgctaaagc tgagcacgta tctgtcgaag atgtgaaagc
      361 gatcattaaa gacaatgaga gggtgcttgt atgaccagac aaagtgtaca agcatttgcc
      421 ggaggaatga ttttggctac cgctgttctt gcaggcgcat tttatttgac gggtaacgga
      481 aaagctgaag cttctaaaga cgctcaaaac atcagcgaaa ccgatgtcaa aacctattta
      541 aaggacaacc agcaggtttc gctgaaacgc gaagaatacc agcagctgct ccattataaa
      601 gagaccgctc tgaagcagga tgcttcgtct caaaacaaaa aagagaaaga agcagaaaaa
      661 aaagattcca agtataagct tgaaatcaaa agcggcatga gcacggctga gatagcagac
      721 ctccttgcaa aaggaaaagt catttcatct gccgatgact tcaaagatta tgtgaaaaag
      781 accggaaacg aatctaaaat ccgcgcaggt aagtacgaac tgaagcgcgg tgacagcttg
      841 aaaaacatcg tcaaaacact gtccaaataa aaaagcaagc tggaacggga ctgacccccg
      901 ttttaagaca ggatttaaaa acacctttaa acaaccaatt cccgatagtt taccggtgat
      961 tggttgttta gttttgtttt aatacgaata ttgttaaaat aatgaatgta tttctttaac
     1021 agtgtgttct acgatggcag tcgtagttcg atcaatgctg ttaagatcaa acgtttcaga
     1081 ctttagtgag gaatgaaacg gatccgatgg aggcattata ttaatagctt tctgatactc
     1141 ataagatgta tacacttaac cttgatcgca cagtaagcaa gggcccatat gtaattttta
     1201 caagctgaag cagtgcgggt acagttcata aaaaggttgt ggctcctgca ccatctaagt
     1261 gtgagatcat cccacttgca gctgtaccca tggaaacagt gacaacattc ccctttgtca
     1321 gctgtttaat gctttgatta gaggttcaaa gaatcctgtg tcttgtagaa tgcaaaaatt
     1381 aaagtgacga agataaacat ggtcgccact ggcagtactt tgataatccc ttctttaaat
     1441 atcattgatg ccaaatccag aaataagcac ccctaccaaa ggcaccatgg tcggacttgc
     1501 tttccccaaa ccagtaaacg ttaacataag ccctccccct acagaaaccg tttacatacc
     1561 tatacaataa aaaaagatgg agaacccatt cgtagtacga agttgaactt gtcaaaaagc
     1621 aattcaaatt attaatttaa acgatataaa aaaacatcca ttaaggacgt tttttgattg
     1681 agaattaagc aatcaattta accaaaagaa agtaactgta attaaaagta ttaaatatat
     1741 ggccgaaaaa attctataaa tacctttttt ggtttggaaa ggtggggata taaaagttac
     1801 taaaatggtc gggccaaaca aagcaatcaa aaagtaccta taatcaaaaa agacagtaca
     1861 gattgatagt agtattgaaa aataaaatac tattataaat gaagtattaa ctttactttt
     1921 cgtttctttt tgttcttctc ctgtatgatc atccagtaat ttatctatct gctttttatt
     1981 aaaaaacata agagcctcct aatgttaatt tgttaagtaa ataaggcgtt attaatgctt
     2041 ctgtaacagc agcaagtaat gtcaataaaa aaagtataat aacaaagtac aaaaaatctt
     2101 gtaaaaactg tttttttgaa aaataaatac cccttatctt catatacacg taatctaaca
     2161 atttgaaacc aagtgctccc gacaaaatca ttgccggaat ctcaaaaata ccgtgaggaa
     2221 ccaaagcagt taatataaat aagacatcgt ttccatggtc agtgatacta tgtaaagaag
     2281 atagaccgac taagaagcca ttcaatagta ataagataga tgtcaaaatt ccaaatgtaa
     2341 acattgaaaa aatgagaaat aaatttgctg caaaattatg tgtaaagtaa cctgatactg
     2401 tgatattttg aacattatct tgactaaggc taggtagaat attgttaaaa atcacaccaa
     2461 gaactaaacc cactatgtaa aaggagcttg ctatatagag tatacgctta tttcttttta
     2521 ttaattcctt cattttagta tcgtatccat atcccttata atcaaatctt caaaatcctt
     2581 tatgtctgtc aggtgattat attctttaac taaagtacca ttaatcagta taccaacctt
     2641 atcgcatagt tgttgcgcta tgtttacaag gtgtgtagac atgatgacag tatttccctg
     2701 ttcagcataa cgtttgagaa aacttttcat catctttgta gtggggggat caaatgcccc
     2761 taatggttca tctaatatta atatttctgg ttcatgaact atggctcctg caacagataa
     2821 cttatgtttc ataccaaaag aaaatgtaga tatcatgtcg ttttcattat ctttcaattt
     2881 aaattgactt aacaaaagat ctattctatc atttttaatc tcataaggga cttcttgtaa
     2941 atcagcaaca aactcaaggt actctctagg tgttaaataa tgatagataa aaggtgcgtc
     3001 tggaacgtat cctatatatt taaggtaaga ggatctttca gattccaagg tttctccttt
     3061 gacagtgatt ttaccttcgt aaggtttcac tataccaagg atacatttta aaagagttga
     3121 tttaccagct ccatttggtc caagtaagcc gtaaactttg cctttctcta tctttaaatt
     3181 aatagattca aggatcagtt tatctttata tttgaaagac acattttcaa tgttcatgtt
     3241 ttcatctccc aatttttcgt tcctattctt ttaatccaaa aataataaaa gactccaata
     3301 cctataacaa ataaaatggt acctgtcaca atgattaaaa agaatactcc cctattaata
     3361 atattaaaag taacgagaac gcctgtgata ccatacaact gaaggtaaaa tgccagtaac
     3421 actccgagaa tagtatgttc aaaaaatgat gctttaacag atgaaccaat gtcttcaaag
     3481 ttctcccaat ctaaacgagg atagagaaat gttccaataa tttgtccagc ccctatgata
     3541 agactgatgg agttaatcat gatgaacaat ataagctgcg tcataatatc taagttgaat
     3601 aacaataaga tgaataaaaa taacgtattc attaaaataa gaatcacaga taagtgcaat
     3661 aaagattttc ctgcgatcaa tttaaaaata gaagcacccg agctcttata aacgtaaagg
     3721 ttctcacgct ctgcatctgc tgaagttacg ccaagtaatc cctctcctaa taaagatgta
     3781 ttataagtca cagcaaataa aattgggtag ataacaaaaa tgtctgagaa atcaaaccac
     3841 ttttgattta aactaagacc tatcaataca cctagttcta ctgcaatcat attaatggaa
     3901 agtaccagtc gtttttttat tgtaaaatta gctcttaata atgcagtaag gtctttcttt
     3961 agaatagcaa tattttgccg tggtaaggtc ttttgtaaga aacttaaaaa aggcaacttg
     4021 attggtttta attttggcaa aagattatta ggtactgctt cttttataat caatactttc
     4081 aaaattaaat gaaaggtaat aaaggcgaaa aggcaaccta aagcaatata acctatagaa
     4141 cataaaaaaa ataaatgtat atcgcttgtt gaaagtaaaa accaatttgt aggcagccaa
     4201 ttggtggtta atatcgtatc taatttgaat agatatatga aattgccaat agcttcgagg
     4261 agatcatttt gataaaaata attaagtccg atccaaccaa gaaaagtagt tgaaatggca
     4321 gatatacagg caattcctat aagggataac aagcttttta atcgtgaatt taaaatgtta
     4381 tgtagaaatt tagaataaac gatgtatgta agcgatgaat aaagacttgt cagtccaaca
     4441 ataaatatta gcagtgatgg aaatgataac aaaagatata tacggtcatg tgtagaaaaa
     4501 tttataaaaa agagtatagg gaataacact actgttaatt taaacaggat gtttagaaaa
     4561 attgttgtaa agaaccttga taataaaatt tttttgggat ccgtttttaa agtatgaaga
     4621 agaagaaaat cttcaggata tacatatcga tgtatgatat cagtcgatga ttttaatata
     4681 ttcaaaacaa aaaagagaat gataaagtta gtacctagag ttagaatatg ctctggaact
     4741 ccagttaaaa gagtctgtga ggctttatat agaataaata tatacaccaa gaaaaagggc
     4801 gcaaagacaa atagaatttt atttgattta agtaaatttt cgcttgatgg gaaaaaataa
     4861 atcaagcgat ttaaagttcg tctataggtt agtttaaagt ttgcaatgag aacccttttg
     4921 ttgagcatgt gtatattcct cctagaacga aaataaggag gagtataatt atactcctca
     4981 atctcttcct tttgagcatt ttaccattca gcagcatatt tcttgccgta ttttttagct
     5041 agctttttcg ctgtagctac cattgcagct gtaattagac cagttccgcc caaaacaaca
     5101 gccaataggc tgagaattgt taatacatcg cctgttgtca taataacacc gattgctaca
     5161 taggctgttt ttttactaat tcctaggttt gttgcaaggt ctaaagcgaa ctgatgacct
     5221 gaactaaagt caatagaagc taaacctgca ataccttctg acgagcccat aaggaaagta
     5281 gcaatggcaa agagagaaac tacacctgta atacttgcaa ttttaaaaag attgctattt
     5341 ttcatcttga aatctcacct cctttcagtt tataaaaata aacttgttga aaaaagagaa
     5401 ataataccta aagacattgt aatgctaatg attatgatgg tttttttata agaaatttga
     5461 tgtattactt ttaaggcaat acaccctaga atagtagacc accaaaagaa agggtcgata
     5521 ataatattca agataatatc tagcggctgt tgagaatttg ggtaaataac tgaaaaggcg
     5581 aaatcactac ctaaaaagaa agaaaaaata atgagtattg taaatttgat gcctataggt
     5641 atgattgaat aaataaaact agggaaaaat tctttggctg tagcggtccc gccaattaat
     5701 atcccaataa tatgtataat aaaaaagctt acaagaacca tcgaggtaaa ttgaataata
     5761 cctaatacta atgtaatgat cataatgagt gtattcatat cagttgtata atcaattcca
     5821 ttgtcttgaa atgaattatt aatcaaggta gagagtgttg ggaagatagc taaataaaag
     5881 cctaagaaca gaaacaatac agctaggaag acaaaccaag aatgacgata attcttatcg
     5941 ctagaatact tgatagaaag aaagtccttt ggagaaaata tgaaatgatg tacgagagta
     6001 ataaagtttt gtttattatc cataaatgct ccttatataa acaaaagtat tttttgttaa
     6061 ttctattaaa aacatatcac cttttacaat gtttggtcaa tatgggattt tatttattaa
     6121 ggtaaattta aggttgaatt gtttcgtgaa aatcgaaaag aatgcctctg ggactacccc
     6181 cttttttaaa acttcatttt gttgtttcag ctaacgactt tctgcctgca ttcagaagaa
     6241 tactcgggat cctttcgata agtatattgt tttccaacag gctgttcgaa tcagtgttta
     6301 tccgcagcct tataccacct gccccatgcc tgaacccgcg ccttattctt gatattcgat
     6361 ttctgcatga tctctttctt aggtacgcct accaatctca tttctaaagc cttccgtttg
     6421 acttcaaccg gaactcactc ttgtccccat agaaaaaaca cctccacgtc ttatttcgga
     6481 taacaactat ccgttcaact ttgaaggtgt tttttatttg tctcatctta tgggaccggt
     6541 ccccttcgta ccagcctgct tttttattgc ctccgctccg gtgcaaaaaa ggctgccgct
     6601 ttaaagcggc agcctctttc tcatccgaac cggcctgtaa tataatcttt tgtccgatag
     6661 tcgtcaggac ttgaaaacat tttgaccgta tcgttgcact caatcagctc gcccatgagg
     6721 aaaaatgccg tttgatcgga aattctcgat gcctgctgca tattgtgcgt gacgattgca
     6781 atcgtgtatt tctctttcag cttgagcatc agctcttcaa ttttcaaagt cgaaactgga
     6841 tcaagagctg atgtcggttc atccatcaag agaacgtccg gattggtggc cagcgccctt
     6901 gcgatgcaga ggcgctgctg ctgccccccg gaaagtccga gcgcctggga atgaaggcgg
     6961 tctttcactt catcccacag cgccacatcc acaagcgctt tttccaccct ctcattcaat
     7021 tcctttttgt ttttcagtcc atggatcctt ggtccgtatg cgacattatc aaaaatcgac
     7081 tggggaaacg gatttccttt ttgaaacacc ataccgactt tcttccgcag ctcaacaaga
     7141 tcgactttgc tgttcagtac gttcgttccg ttgtagctga tctctccggt cattttgaca
     7201 ttcggaacca tattgatcat gagattcagc gttttaatga aggtcgactt tccgcagcct
     7261 gacggaccga tgatggcggt aatttcgtac tcgggtattg tcaagttaat gttctttaat
     7321 gcgtgatgtt cgccgtacca taaattcaaa tcattgatct ggtacacatc ttttggcttt
     7381 acggctgtta cagcgctcat accggcccct ccttatccaa acctgccgtt gatgtaatcc
     7441 tctgtctttt tttctgaagg attcataaag atcttttccg tacgatcgta ttcgatcaaa
     7501 tctccgttaa ggaaaaatgc cgtttgatcg gaaattcttg aagcttgctg catgttatgc
     7561 gtgacaatga tgatcgagta ttcctctttt aatccgacga ttaattcctc tattttcgca
     7621 tttgaaatcg gatcgagcgc tgaagcaggc tcatccaaaa gcaggacttc aggcttcatc
     7681 gcgagcgtcc gggcgatgca gaggcgctgc tgctgcccgc ctgacagcga aaaagccgac
     7741 tcatgcagcc tgtctttcac ttcatcccac agcgcggctt tcctcaagct ttcttccacg
     7801 atctcatcaa gcacggattt acgccgttcg cccgcatact ttaaagcatg ggtaatgttg
     7861 ctgtaaatcg attttggaaa gggattcggc ttctgaaata ccattccgat ttcccttctc
     7921 agattgacga cattgatatt ggaatcaagg atgttaagtc cttcatatcg tatttctccg
     7981 gatgttctgg ctgatggaat cagatcgttc atccggttga tgcttcttaa aaaagtcgat
     8041 tttccgcagc ctgacgggcc gatcaaagcc gtaacggcat gtttgtcaac atgcatggaa
     8101 atgtcgttga cggcccgctt ttctccatag taaatattta aatttttcac ttctaaaata
     8161 tgttctttcg cttcaggaaa cggagcgatt ccgatagtgt cgcgcacggt ttccaaaacc
     8221 ggcatctgaa cctgaaccat atgatttcat ccttccttct tatttgtttg ccgttaactt
     8281 tttatgaata aaagagccga gccatcgggc tgccaaatta aagatcaaga cggacagtac
     8341 aagaacggcg gatgccccgt ttgcgatcgc ttcagcatca gggatgatcc cctgtgtgtt
     8401 tacattccaa atgtgaacgg ccagcgtttc agccggtctg aaaatattga gcggcgaggt
     8461 ctcagaaaac ggattccaat cggcgaaatt cagtctcggc gtggttagtc cggctgtaaa
     8521 cagcaaagcg gcagcttcgc caaatacgcg gcccgacgca agaatcgccc ctgtgatgat
     8581 ggacggcacc gcaccaggga tcaagaccgt tttaatcgta tgccacttgg acacacctaa
     8641 agcaagggaa gcttcttttt gttccttagg tacagagcgg atcgcatcct ctgtcaccct
     8701 gaccatgacg ggaaggttga aaatcgtcaa agcaagcgct ccgccgataa tgctgtaccc
     8761 ccatccggtc aaattaacaa acatcagcat gccgaacatc cccatgacga tcgaaggaag
     8821 ggatgacaaa acttcaatac agacacggat aaaatcggtc aatttgttgg cgggcgcata
     8881 ttcagccata taaattcctc cgccgactcc aaggggaact gtgatcagca tcgtcagtgc
     8941 gagaatataa aacgagttga acagctgatc gcggattccg ccgccgcttg aaagcgcact
     9001 ggaacgcgaa gttaaaaagc tgaggctgat atgtgaaaca ccattgatga tgatataagt
     9061 gaacaaaccg acaagaatgg cggcgattac cgcagcaatc aatccaaaaa caccggttgc
     9121 taaacgatct gttactttgc tgttcattac agtttcctcc tggatgacag atagcggatg
     9181 agcaggatga acaagaacga catgatcaga aggacaaggc ccattgacca gagcgtattg
     9241 ttttccacgc ttccgtatgt tgtatggccc atattcaacg ttataatcgt tgttaatgtc
     9301 cccgcagggt ctaaaaggct ctcaaacagg tttcgggaat ttccgatgac catctgcacg
     9361 gccagcgctt cgccgaacgc cctcgccatt cctaagacga cggctgtcag aagcgaaggc
     9421 agcgctgccg gtatcagcac ttttcggatc gtctgccagc gggttgcgcc caatgcgtat
     9481 gatccttcgc gcagattttt cggcagcgac atgagcgcat ccgttgaaat agaagtgacc
     9541 gtcggcaaaa tcatgacagc caggacgatc gttcccgcca gaaggctgtg gcccgatccg
     9601 cttgacttaa actgtgaaat gaacggcact aacactgtaa ggcctatgaa tccataaacg
     9661 acggaaggaa taccaacaag caattcgaca accggctgca gtacttttct gccccatgat
     9721 ggcgcgattt ccgtcatgaa aatcgcacct cctattccaa gcggtgcagc aatcaaagat
     9781 gcaagaaggg tcactgcaaa agatccgagt ataaacggaa gggctccgta ttcagggttt
     9841 tcgttcgttg gattccaatc tagactggtt aagaactcaa ttggacttac accattggcc
     9901 agaaaagatt gcaacccctt atttccgaga aaaattgtga tcgaaatcgc cgccgccatc
     9961 atgataaacg cgcacgacgc aaccaaaatc ctcccgcgga tttcatccat tttgcgatta
    10021 tgcttggagc tgatcagccg ctcttttgcg gacagtttct ctattttttg aaccattttt
    10081 ttacactcct ataagaacaa gtcataagag ggaaagtgtc aggagcacac tttcccgcct
    10141 tctattgtca attatttatc agtttgcttt ccattcgcat cgcgcttgac ttccatgtct
    10201 gttactggaa tgtagccctg ctccgtcacg atggattttt gaatgtcatc gttcatcaaa
    10261 tagtcgagga attctttcgc caagccttca ggctcgcctt tcgtataaga atgctggtat
    10321 gcccagatcg ggaatttgcc ttttt
//
