LOCUS       NZ_NKBC01000003        10045 bp    DNA     linear   CON 17-MAR-2024
DEFINITION  Bacillus paralicheniformis strain 9-1 9-1_R1_(paired)_contig_3,
            whole genome shotgun sequence.
ACCESSION   NZ_NKBC01000003
VERSION     NZ_NKBC01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus paralicheniformis
  ORGANISM  Bacillus paralicheniformis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Cui,Y.
  TITLE     Improper usage of probiotics constitutes a potential risk of public
            health
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10045)
  AUTHORS   Cui,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2017) Beijing Advanced Innovation Center for Food
            Nutrition and Human Health, College of Veterinary Medicine, China
            Agricultural University, Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 9.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009935535.1-RS_2024_03_17
            Annotation Date                   :: 03/17/2024 02:24:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,539
            CDSs (total)                      :: 4,461
            Genes (coding)                    :: 4,361
            CDSs (with protein)               :: 4,361
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 1 (5S, 16S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 49 of 100
            Pseudo Genes (incomplete)         :: 64 of 100
            Pseudo Genes (internal stop)      :: 15 of 100
            Pseudo Genes (multiple problems)  :: 21 of 100
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:47:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10045
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NKBC01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..320
                     /locus_tag="CE036_RS03475"
     source          1..10045
                     /collection_date="2016-06"
                     /country="China"
                     /db_xref="taxon:1648923"
                     /host="Homo sapiens"
                     /mol_type="genomic DNA"
                     /note="commercial probiotic used for human"
                     /organism="Bacillus paralicheniformis"
                     /strain="9-1"
                     /submitter_seqid="9-1_R1_(paired)_contig_3"
     region          1..10045
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..10045
                     /SMILES="NC(CCCCN)C(=O)NC(CCCN)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10045
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[436:>10045](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      437..>10045
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             <5..320
                     /NRPS_PKS="Domain: PCP (21-88). E-value: 3.7e-20. Score:
                     63.9. Matches aSDomain: nrpspksdomains_CE036_RS03475_PCP.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /locus_tag="CE036_RS03475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_161517305.1"
                     /sec_met_domain="PP-binding (E-value: 1.7e-16, bitscore:
                     48.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LPEPDRSAGTEAEYEAPRNYVEQRIISILEDVLGTERMGISCHFF
                     DKGGNSLKAMQAVHSINKTFGLNMRISTFFKHPTAKSLARFVLTAEAESAVSEEYAEEE
                     V"
     aSDomain        69..269
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03475_PCP.1"
                     /evalue="3.70E-20"
                     /label="CE036_RS03475_PCP.1"
                     /locus_tag="CE036_RS03475"
                     /protein_end="88"
                     /protein_start="21"
                     /score="63.9"
                     /tool="antismash"
                     /translation="EQRIISILEDVLGTERMGISCHFFDKGGNSLKAMQAVHSINKTFG
                     LNMRISTFFKHPTAKSLARFVL"
     gene            437..8254
                     /gene="bacB"
                     /locus_tag="CE036_RS03480"
     CDS             437..8254
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (72-357). E-value:
                     4.2e-103. Score: 336.4. Matches aSDomain:
                     nrpspksdomains_CE036_RS03480_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (523-911). E-value:
                     2.2e-111. Score: 364.3. Matches aSDomain:
                     nrpspksdomains_CE036_RS03480_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (1019-1087). E-value: 8.8e-24.
                     Score: 75.5. Matches aSDomain:
                     nrpspksdomains_CE036_RS03480_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1102-1388). E-value:
                     4.3e-110. Score: 359.3. Matches aSDomain:
                     nrpspksdomains_CE036_RS03480_Condensation_LCL.2"
                     /NRPS_PKS="Domain: AMP-binding (1553-1955). E-value:
                     2.2e-115. Score: 377.5. Matches aSDomain:
                     nrpspksdomains_CE036_RS03480_AMP-binding.2"
                     /NRPS_PKS="Domain: PCP (2062-2130). E-value: 6.8e-23.
                     Score: 72.7. Matches aSDomain:
                     nrpspksdomains_CE036_RS03480_PCP.2"
                     /NRPS_PKS="Domain: Epimerization (2140-2441). E-value:
                     1.9e-153. Score: 504.0. Matches aSDomain:
                     nrpspksdomains_CE036_RS03480_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (2445-2596). E-value: 1.9e-45.
                     Score: 146.4. Matches aSDomain:
                     nrpspksdomains_CE036_RS03480_TIGR01720.1"
                     /codon_start=1
                     /gene="bacB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039072839.1"
                     /locus_tag="CE036_RS03480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacitracin non-ribosomal peptide synthetase BacB"
                     /protein_id="WP_161517306.1"
                     /sec_met_domain="PP-binding (E-value: 1.4e-18, bitscore:
                     55.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.9e-93, bitscore:
                     300.4, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.7e-122, bitscore:
                     395.9, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIMDFINDLKKKNITLYHNKGKIKIIGPQELLTADLKQQIKRYK
                     DDIIAALEAGETDIERSFPKAAPSKSGTYPLSREQKRMFILNQLDDSKTAYNMPMAVKI
                     NGEVQISRIEQAWKALIKRHESLRTSFVMLDGEPVQKIEQEAEFRLEYSELGDQSIQEK
                     ISRFIKPFQLEKAPLLRAEIVKVDEAEHMMIVDMHHIISDGISIGILMKEFADCCEGKE
                     LPPVAVQYKDYSEWQKDIEQQSRLKKQEAYWLNTFRGDIPVLNMPLDFPRPKIRSFQGN
                     RTVVELDQDTTKKLKTIAAKNGVTMYMLLLAGYTILLSKYTGQEDIIVGSPIAGRPHAD
                     LNGTIGMFVGTLALRNRPTGNMTFSEYVQTVKNNTLNAYENQDYQFDALIEHLGLTQDM
                     SRNPLFDTMFDLQHADDFVSEAGGGHFDTYDIPFHVAKFDVSLTAFLHGDNLKFDFQYC
                     TDLYKKETIERMAGHFLNVLKDAAHHPELALSEIRMMSEEEKDIILHTFNHEKTDGPKN
                     KTLSRLFEERAEKTPDHPAVIFEDQQLTYRELNEKANQLAWLLREKGVKPDTIVAIMTD
                     RSLEMIIGIIGILKAGGAYLPIDPDYPEDRVKYMLEDSGADMVVIQEPFKSKIDGRQLI
                     TAEDTRSFSKENLPNVNKASDLAYVIYTSGSSGRPKGVMTTHRNVVHYVDAFTKRIPLS
                     EDDTVLQVVSFSFDAFSEEVYPILACSGRLVISRKVSDLNIDELVKTIGKHRVTLVSCS
                     PLLLNEIDKNQHLTFHPQMKFISGGDVLKFEYVENIIKGADVYNSYGPTEATVCATYYQ
                     LSSADRKKTSIPIGKPLSNYKVYIADQYGRPQPVGVPGELLIGGEGVARGYLNHETLTK
                     AAFVLDESGERVYRTGDLARWLSDGNIEFLGRIDSQVKIRGYRIELEEIEHRLLMNDDI
                     NEAIVVAKEDQENSKYLCAYIAFNNKNADIEQVQERLAKDLPEYMIPSCFIKLDQIPRT
                     INGKADLKALPEPDRGAFAQARYEAPRNQTEALLLSIWQDILPAEQIGINDHFFDIGGH
                     SLKAFSMAAKIQAALKVEVTLKEIFSHPTIKDIAAYIVQKQKQVQSDILKAKKKEYYPL
                     SSAQKRLYILNQIEEGQTAYNMPFAMKIRGGLQTKKAEKAFKTLIKRHESLRTSFVTIN
                     GEPVQNINEGVTFEMKYRELDNCSLRERMNQFIRPFDLEKAPLLRAELVRVNAAEHILL
                     LDMHHIISDGVSIGILMKEWAALYEEKELAPLKIQYKDYSEWQRESCQKDRLKKQEEYW
                     LSVFQDAIPVLNMPTDFPRPQMQSYEGDRIAFAIERELTDKLKKTAKENGVTMYMLLLA
                     GYTILLSKYTGQEDIIVGSPIAGRTREELEQTVGMFVGTLAMRNHPKGGRTFIEYLQDV
                     KENTFNAYENQDYPFDELVDKLDLERDISRNALFDTMFDMQALDDAEPDIEGLHVEPVD
                     LEFQISKFDLSLAAAESAGVITFHLEFCTRLYKKETAETLAQHFVNILRDISDHPQKPL
                     NEISMLSEEQRNTVLYQFNDTNAEHPSGIFSELFEEQAEKSPNHPAAVFKDHMLTYREL
                     NEKANQLARTLRKKGVQRESVVGIMAERSLEMVTGILAVLKAGGAYMPIDPGLPKERIQ
                     YLITDSGADLLLTQHQLIGSISFAGEIIQIDQADAYDTDGSNLEHLNSPGDLAYVIYTS
                     GTTGNPKGVMVEHRNIIHAHYTWRKHYELASFSVNLLQLASMSFDVFAGDLCRSLLNGG
                     TMYMVPDDVKLEMNLLYDMINKYGIHMLESTPSLIIPLMKYIDHHKLDLSSMKLLIMGS
                     DTCTIKDYKWLVERFGQRMRIINSYGVTEASVDSGYYEEALDRIPEIANTPIGKPLDNT
                     AFYILDSSLNPQPVGVYGELYIGGEGVARGYLNKPELTKERFVPNRFAAGGKMYKTGDL
                     ARWLPDGNVEFLGRIDHQVKIRGFRIETGEIETKLLENQNISEAVVIDREDKKGHKYLC
                     AYIVARAKTNANELREYLSDHLPDYMLPSYFIQINKMPLTPNGKIDRKALPEPAGDAIA
                     ANGYKAPRNETEEKLAAVWQEVLDRDKIGINDNFFEIGGDSIKALQIVSKLSRADLKLQ
                     VKDLFTNPSIRHLSKYVKKETKARTSEIVQGQVPLTPVQRSFFEANQREQNHYNQAFML
                     YRENGFAERIVEKVIRKLTEHHDALRMVYREKNGEIIQHNRGLEDSVFDLNVYDLKTEK
                     NLEETVYQIATNIQKDISISEGKMIKLCVFKTTEGDHLLIAIHHLLVDGVSWRILFEDF
                     EAAYGQALQGKPIELGYKTDSYKTFSEKLAEYASSKKLLKEQEYWREISKGKMAFLPKH
                     RQAAHDNYENSRTLRISLSQMETEQLLKNAHKAYNTQINDLLLTALLIASRQLTGENRL
                     KILMEGHGRDDILQDVDITRTVGWFTAMYPVFIDLEDETDLSVMIKIVKETLRKIPNNG
                     IGYGILKYLRKDEGLIKDEKPPILFNYLGELDHDLTTEQFSSSKLSTGQSIGEKSARDA
                     SVEIDSVVVGRQLMISTTFNEYEYSPDTISELNQAFKESLQMVISHCTGKNETEKTSSD
                     YGYDKLSLEDLEELLNEYESIDS"
     aSDomain        653..1507
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03480_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.20E-103"
                     /label="CE036_RS03480_Condensation_LCL.1"
                     /locus_tag="CE036_RS03480"
                     /protein_end="357"
                     /protein_start="72"
                     /score="336.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="TYPLSREQKRMFILNQLDDSKTAYNMPMAVKINGEVQISRIEQAW
                     KALIKRHESLRTSFVMLDGEPVQKIEQEAEFRLEYSELGDQSIQEKISRFIKPFQLEKA
                     PLLRAEIVKVDEAEHMMIVDMHHIISDGISIGILMKEFADCCEGKELPPVAVQYKDYSE
                     WQKDIEQQSRLKKQEAYWLNTFRGDIPVLNMPLDFPRPKIRSFQGNRTVVELDQDTTKK
                     LKTIAAKNGVTMYMLLLAGYTILLSKYTGQEDIIVGSPIAGRPHADLNGTIGMFVGTLA
                     LRNR"
     aSModule        653..3697
                     /complete
                     /domains="nrpspksdomains_CE036_RS03480_Condensation_LCL.1"
                     /domains="nrpspksdomains_CE036_RS03480_AMP-binding.1"
                     /domains="nrpspksdomains_CE036_RS03480_PCP.1"
                     /locus_tags="CE036_RS03480"
                     /monomer_pairings="Lys -> Lys"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       716..832
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0001"
                     /evalue="9.40E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE036_RS03480"
                     /protein_end="132"
                     /protein_start="93"
                     /score="56.6"
                     /tool="antismash"
                     /translation="TAYNMPMAVKINGEVQISRIEQAWKALIKRHESLRTSFV"
     CDS_motif       1025..1087
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0002"
                     /evalue="3.50E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="CE036_RS03480"
                     /protein_end="217"
                     /protein_start="196"
                     /score="29.2"
                     /tool="antismash"
                     /translation="MHHIISDGISIGILMKEFADC"
     CDS_motif       1121..1153
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0003"
                     /evalue="8.90E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="CE036_RS03480"
                     /protein_end="239"
                     /protein_start="228"
                     /score="12.0"
                     /tool="antismash"
                     /translation="QYKDYSEWQKD"
     CDS_motif       1412..1501
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0004"
                     /evalue="1.00E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="CE036_RS03480"
                     /protein_end="355"
                     /protein_start="325"
                     /score="43.6"
                     /tool="antismash"
                     /translation="DIIVGSPIAGRPHADLNGTIGMFVGTLALR"
     CDS_motif       1553..1669
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0005"
                     /evalue="5.40E-16"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CE036_RS03480"
                     /protein_end="411"
                     /protein_start="372"
                     /score="50.9"
                     /tool="antismash"
                     /translation="NNTLNAYENQDYQFDALIEHLGLTQDMSRNPLFDTMFDL"
     aSDomain        2006..3169
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03480_AMP-binding.1"
                     /evalue="2.20E-111"
                     /label="CE036_RS03480_AMP-binding.1"
                     /locus_tag="CE036_RS03480"
                     /protein_end="911"
                     /protein_start="523"
                     /score="364.3"
                     /specificity="substrate consensus: Lys"
                     /tool="antismash"
                     /translation="FEERAEKTPDHPAVIFEDQQLTYRELNEKANQLAWLLREKGVKPD
                     TIVAIMTDRSLEMIIGIIGILKAGGAYLPIDPDYPEDRVKYMLEDSGADMVVIQEPFKS
                     KIDGRQLITAEDTRSFSKENLPNVNKASDLAYVIYTSGSSGRPKGVMTTHRNVVHYVDA
                     FTKRIPLSEDDTVLQVVSFSFDAFSEEVYPILACSGRLVISRKVSDLNIDELVKTIGKH
                     RVTLVSCSPLLLNEIDKNQHLTFHPQMKFISGGDVLKFEYVENIIKGADVYNSYGPTEA
                     TVCATYYQLSSADRKKTSIPIGKPLSNYKVYIADQYGRPQPVGVPGELLIGGEGVARGY
                     LNHETLTKAAFVLDESGERVYRTGDLARWLSDGNIEFLGRIDSQVKIR"
     CDS_motif       2195..2233
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0006"
                     /evalue="1.10E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="CE036_RS03480"
                     /protein_end="599"
                     /protein_start="586"
                     /score="15.5"
                     /tool="antismash"
                     /translation="GILKAGGAYLPID"
     CDS_motif       2408..2467
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0007"
                     /evalue="1.90E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="CE036_RS03480"
                     /protein_end="677"
                     /protein_start="657"
                     /score="29.5"
                     /tool="antismash"
                     /translation="AYVIYTSGSSGRPKGVMTTH"
     CDS_motif       2828..2857
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0008"
                     /evalue="1.50E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="CE036_RS03480"
                     /protein_end="807"
                     /protein_start="797"
                     /score="8.3"
                     /tool="antismash"
                     /translation="SYGPTEATVC"
     CDS_motif       2969..3058
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0009"
                     /evalue="2.30E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="CE036_RS03480"
                     /protein_end="874"
                     /protein_start="844"
                     /score="45.5"
                     /tool="antismash"
                     /translation="PVGVPGELLIGGEGVARGYLNHETLTKAAF"
     CDS_motif       3134..3199
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0010"
                     /evalue="2.90E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="CE036_RS03480"
                     /protein_end="921"
                     /protein_start="899"
                     /score="35.3"
                     /tool="antismash"
                     /translation="FLGRIDSQVKIRGYRIELEEIE"
     aSDomain        3494..3697
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03480_PCP.1"
                     /evalue="8.80E-24"
                     /label="CE036_RS03480_PCP.1"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1087"
                     /protein_start="1019"
                     /score="75.5"
                     /tool="antismash"
                     /translation="EALLLSIWQDILPAEQIGINDHFFDIGGHSLKAFSMAAKIQAALK
                     VEVTLKEIFSHPTIKDIAAYIVQ"
     aSDomain        3743..4600
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03480_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.30E-110"
                     /label="CE036_RS03480_Condensation_LCL.2"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1388"
                     /protein_start="1102"
                     /score="359.3"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="EYYPLSSAQKRLYILNQIEEGQTAYNMPFAMKIRGGLQTKKAEKA
                     FKTLIKRHESLRTSFVTINGEPVQNINEGVTFEMKYRELDNCSLRERMNQFIRPFDLEK
                     APLLRAELVRVNAAEHILLLDMHHIISDGVSIGILMKEWAALYEEKELAPLKIQYKDYS
                     EWQRESCQKDRLKKQEEYWLSVFQDAIPVLNMPTDFPRPQMQSYEGDRIAFAIERELTD
                     KLKKTAKENGVTMYMLLLAGYTILLSKYTGQEDIIVGSPIAGRTREELEQTVGMFVGTL
                     AMRNH"
     aSModule        3743..8224
                     /complete
                     /domains="nrpspksdomains_CE036_RS03480_Condensation_LCL.2"
                     /domains="nrpspksdomains_CE036_RS03480_AMP-binding.2"
                     /domains="nrpspksdomains_CE036_RS03480_PCP.2"
                     /domains="nrpspksdomains_CE036_RS03480_Epimerization.1"
                     /domains="nrpspksdomains_CE036_RS03480_TIGR01720.1"
                     /locus_tags="CE036_RS03480"
                     /monomer_pairings="Orn -> D-Orn"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       3809..3925
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0011"
                     /evalue="7.40E-14"
                     /label="C2_LCL_024-062"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1163"
                     /protein_start="1124"
                     /score="44.1"
                     /tool="antismash"
                     /translation="TAYNMPFAMKIRGGLQTKKAEKAFKTLIKRHESLRTSFV"
     CDS_motif       4118..4183
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0012"
                     /evalue="6.90E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1249"
                     /protein_start="1227"
                     /score="31.4"
                     /tool="antismash"
                     /translation="MHHIISDGVSIGILMKEWAALY"
     CDS_motif       4214..4246
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0013"
                     /evalue="1.00E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1270"
                     /protein_start="1259"
                     /score="14.9"
                     /tool="antismash"
                     /translation="QYKDYSEWQRE"
     CDS_motif       4505..4594
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0014"
                     /evalue="1.50E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1386"
                     /protein_start="1356"
                     /score="46.3"
                     /tool="antismash"
                     /translation="DIIVGSPIAGRTREELEQTVGMFVGTLAMR"
     CDS_motif       4643..4762
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0015"
                     /evalue="8.10E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1442"
                     /protein_start="1402"
                     /score="56.8"
                     /tool="antismash"
                     /translation="KENTFNAYENQDYPFDELVDKLDLERDISRNALFDTMFDM"
     aSDomain        5096..6301
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03480_AMP-binding.2"
                     /evalue="2.20E-115"
                     /label="CE036_RS03480_AMP-binding.2"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1955"
                     /protein_start="1553"
                     /score="377.5"
                     /specificity="substrate consensus: Orn"
                     /tool="antismash"
                     /translation="FEEQAEKSPNHPAAVFKDHMLTYRELNEKANQLARTLRKKGVQRE
                     SVVGIMAERSLEMVTGILAVLKAGGAYMPIDPGLPKERIQYLITDSGADLLLTQHQLIG
                     SISFAGEIIQIDQADAYDTDGSNLEHLNSPGDLAYVIYTSGTTGNPKGVMVEHRNIIHA
                     HYTWRKHYELASFSVNLLQLASMSFDVFAGDLCRSLLNGGTMYMVPDDVKLEMNLLYDM
                     INKYGIHMLESTPSLIIPLMKYIDHHKLDLSSMKLLIMGSDTCTIKDYKWLVERFGQRM
                     RIINSYGVTEASVDSGYYEEALDRIPEIANTPIGKPLDNTAFYILDSSLNPQPVGVYGE
                     LYIGGEGVARGYLNKPELTKERFVPNRFAAGGKMYKTGDLARWLPDGNVEFLGRIDHQV
                     KIR"
     CDS_motif       5282..5323
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0016"
                     /evalue="6.70E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1629"
                     /protein_start="1615"
                     /score="16.1"
                     /tool="antismash"
                     /translation="LAVLKAGGAYMPID"
     CDS_motif       5507..5566
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0017"
                     /evalue="1.00E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1710"
                     /protein_start="1690"
                     /score="33.3"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGNPKGVMVEH"
     CDS_motif       5951..5977
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0018"
                     /evalue="8.20E+00"
                     /label="NRPS-A_a5"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1847"
                     /protein_start="1838"
                     /score="0.5"
                     /tool="antismash"
                     /translation="SYGVTEASV"
     CDS_motif       6095..6184
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0019"
                     /evalue="7.20E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1916"
                     /protein_start="1886"
                     /score="53.4"
                     /tool="antismash"
                     /translation="PVGVYGELYIGGEGVARGYLNKPELTKERF"
     CDS_motif       6266..6331
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0020"
                     /evalue="9.80E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CE036_RS03480"
                     /protein_end="1965"
                     /protein_start="1943"
                     /score="36.8"
                     /tool="antismash"
                     /translation="FLGRIDHQVKIRGFRIETGEIE"
     aSDomain        6623..6826
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03480_PCP.2"
                     /evalue="6.80E-23"
                     /label="CE036_RS03480_PCP.2"
                     /locus_tag="CE036_RS03480"
                     /protein_end="2130"
                     /protein_start="2062"
                     /score="72.7"
                     /tool="antismash"
                     /translation="EEKLAAVWQEVLDRDKIGINDNFFEIGGDSIKALQIVSKLSRADL
                     KLQVKDLFTNPSIRHLSKYVKKE"
     aSDomain        6857..7759
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03480_Epimerization.1"
                     /evalue="1.90E-153"
                     /label="CE036_RS03480_Epimerization.1"
                     /locus_tag="CE036_RS03480"
                     /protein_end="2441"
                     /protein_start="2140"
                     /score="504.0"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLLVDG"
                     /tool="antismash"
                     /translation="GQVPLTPVQRSFFEANQREQNHYNQAFMLYRENGFAERIVEKVIR
                     KLTEHHDALRMVYREKNGEIIQHNRGLEDSVFDLNVYDLKTEKNLEETVYQIATNIQKD
                     ISISEGKMIKLCVFKTTEGDHLLIAIHHLLVDGVSWRILFEDFEAAYGQALQGKPIELG
                     YKTDSYKTFSEKLAEYASSKKLLKEQEYWREISKGKMAFLPKHRQAAHDNYENSRTLRI
                     SLSQMETEQLLKNAHKAYNTQINDLLLTALLIASRQLTGENRLKILMEGHGRDDILQDV
                     DITRTVGWFTAMYPVFIDLE"
     CDS_motif       6995..7027
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0021"
                     /evalue="3.90E-02"
                     /label="Cy2"
                     /locus_tag="CE036_RS03480"
                     /protein_end="2197"
                     /protein_start="2186"
                     /score="6.8"
                     /tool="antismash"
                     /translation="LTEHHDALRMV"
     CDS_motif       7232..7297
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0022"
                     /evalue="1.40E-10"
                     /label="NRPS-E2"
                     /locus_tag="CE036_RS03480"
                     /protein_end="2287"
                     /protein_start="2265"
                     /score="33.4"
                     /tool="antismash"
                     /translation="LLIAIHHLLVDGVSWRILFEDF"
     CDS_motif       7700..7750
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0023"
                     /evalue="2.10E-08"
                     /label="NRPS-E5"
                     /locus_tag="CE036_RS03480"
                     /protein_end="2438"
                     /protein_start="2421"
                     /score="26.2"
                     /tool="antismash"
                     /translation="DITRTVGWFTAMYPVFI"
     aSDomain        7772..8224
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03480_TIGR01720.1"
                     /evalue="1.90E-45"
                     /label="CE036_RS03480_TIGR01720.1"
                     /locus_tag="CE036_RS03480"
                     /protein_end="2596"
                     /protein_start="2445"
                     /score="146.4"
                     /tool="antismash"
                     /translation="LSVMIKIVKETLRKIPNNGIGYGILKYLRKDEGLIKDEKPPILFN
                     YLGELDHDLTTEQFSSSKLSTGQSIGEKSARDASVEIDSVVVGRQLMISTTFNEYEYSP
                     DTISELNQAFKESLQMVISHCTGKNETEKTSSDYGYDKLSLEDLEEL"
     CDS_motif       7793..7855
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03480_0024"
                     /evalue="8.70E-11"
                     /label="NRPS-E6"
                     /locus_tag="CE036_RS03480"
                     /protein_end="2473"
                     /protein_start="2452"
                     /score="33.9"
                     /tool="antismash"
                     /translation="VKETLRKIPNNGIGYGILKYL"
     gene            8368..>10045
                     /locus_tag="CE036_RS03485"
     CDS             8368..>10045
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (1-27). E-value: 1.5e-13.
                     Score: 41.7. Matches aSDomain:
                     nrpspksdomains_CE036_RS03485_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (5-301). E-value:
                     8.3e-113. Score: 368.4. Matches aSDomain:
                     nrpspksdomains_CE036_RS03485_Condensation_DCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="CE036_RS03485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_161517307.1"
                     /sec_met_domain="Condensation (E-value: 3.9e-88, bitscore:
                     283.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-23, bitscore:
                     71.0, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKVEKIYPLSNMQKGMLFHAMKDEASHAYFEQFIIELKGDVDE
                     RMFEESLNEVMKRHEILRASFHHRLDEPLHVIIKDRHMKFDYLDIRGRHEQDGVLESYL
                     AEDRQKGFNLAKDTLMRACLIRTSDDSYQFVWTYHHILLDGWCLGIILDELLTIYEMKR
                     KGQHHQLEDPRPYSDYIKWLEDQDKEEAQSYWESYLSGYDQKNSLPKLRTPSETGFKRR
                     EKTIECSKELTNRLIKLANQNHVTINTVLQSIWGVILAKYNNSEDVVFGTVVSGRDAEV
                     EGIETMVGVFINTIPTRIRLGKDKLFKDVLRQTQADALESSRYNYMNLAEVQALSELKN
                     DLIDHVMVFENYAVDQKAFEEKNDVGFEMVNVSGEEQTNYHFSISAALDDQLKLLFIYD
                     ENVYDDTIIETLEKHIITVAEQVAEDEAQTLQDINLVSKEEQQRILDTFNDTKTDYPKD
                     KPLHELFEEQAMKTPDQTALVFGAQRMTYRELNKKANQTARLLREKGIGRGSIAAIIAD
                     RSFEMIIGIIGILKAGGTYLPIDPETPRDRIDYMLENSGAAL"
     aSDomain        8371..8448
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03485_NRPS-COM_Nterm.1"
                     /evalue="1.50E-13"
                     /label="CE036_RS03485_NRPS-COM_Nterm.1"
                     /locus_tag="CE036_RS03485"
                     /protein_end="27"
                     /protein_start="1"
                     /score="41.7"
                     /tool="antismash"
                     /translation="KTKVEKIYPLSNMQKGMLFHAMKDEA"
     aSDomain        8383..9270
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE036_RS03485_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="8.30E-113"
                     /label="CE036_RS03485_Condensation_DCL.1"
                     /locus_tag="CE036_RS03485"
                     /protein_end="301"
                     /protein_start="5"
                     /score="368.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHILLDG"
                     /tool="antismash"
                     /translation="EKIYPLSNMQKGMLFHAMKDEASHAYFEQFIIELKGDVDERMFEE
                     SLNEVMKRHEILRASFHHRLDEPLHVIIKDRHMKFDYLDIRGRHEQDGVLESYLAEDRQ
                     KGFNLAKDTLMRACLIRTSDDSYQFVWTYHHILLDGWCLGIILDELLTIYEMKRKGQHH
                     QLEDPRPYSDYIKWLEDQDKEEAQSYWESYLSGYDQKNSLPKLRTPSETGFKRREKTIE
                     CSKELTNRLIKLANQNHVTINTVLQSIWGVILAKYNNSEDVVFGTVVSGRDAEVEGIET
                     MVGVFINTIPTRIRL"
     CDS_motif       8392..8433
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03485_0001"
                     /evalue="1.80E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="CE036_RS03485"
                     /protein_end="22"
                     /protein_start="8"
                     /score="16.5"
                     /tool="antismash"
                     /translation="YPLSNMQKGMLFHA"
     CDS_motif       8452..8571
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03485_0002"
                     /evalue="1.30E-16"
                     /label="C2_DCL_024-062"
                     /locus_tag="CE036_RS03485"
                     /protein_end="68"
                     /protein_start="28"
                     /score="52.8"
                     /tool="antismash"
                     /translation="HAYFEQFIIELKGDVDERMFEESLNEVMKRHEILRASFHH"
     CDS_motif       8779..8844
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03485_0003"
                     /evalue="7.90E-12"
                     /label="C3_DCL_135-156"
                     /locus_tag="CE036_RS03485"
                     /protein_end="159"
                     /protein_start="137"
                     /score="37.5"
                     /tool="antismash"
                     /translation="YHHILLDGWCLGIILDELLTIY"
     CDS_motif       8890..8928
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03485_0004"
                     /evalue="5.40E-04"
                     /label="C4_DCL_171-183"
                     /locus_tag="CE036_RS03485"
                     /protein_end="187"
                     /protein_start="174"
                     /score="12.4"
                     /tool="antismash"
                     /translation="PYSDYIKWLEDQD"
     CDS_motif       9166..9261
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03485_0005"
                     /evalue="4.80E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="CE036_RS03485"
                     /protein_end="298"
                     /protein_start="266"
                     /score="60.8"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRDAEVEGIETMVGVFINTIPTR"
     CDS_motif       9310..9423
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03485_0006"
                     /evalue="4.50E-16"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="CE036_RS03485"
                     /protein_end="352"
                     /protein_start="314"
                     /score="51.2"
                     /tool="antismash"
                     /translation="QADALESSRYNYMNLAEVQALSELKNDLIDHVMVFENY"
     CDS_motif       9949..9987
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CE036_RS03485_0007"
                     /evalue="8.50E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="CE036_RS03485"
                     /protein_end="540"
                     /protein_start="527"
                     /score="15.8"
                     /tool="antismash"
                     /translation="GILKAGGTYLPID"
ORIGIN
        1 ccctgcctga gccggacaga agcgcaggaa ctgaagcgga gtacgaagcg ccaagaaact
       61 atgtcgaaca gcgcatcatt tccatcttgg aggacgtact cggaacagag cggatgggta
      121 tttcctgcca tttcttcgac aaggggggga attcgctgaa agcgatgcaa gctgttcatt
      181 ccattaacaa aacgtttggc cttaatatga ggatcagcac gttctttaaa catccgactg
      241 caaaatcatt agcgcgtttt gtattaaccg ctgaagccga atcggccgtt agtgaggagt
      301 acgcggaaga ggaagtgtga tggaattgca ctgtacaatc tatcatttga aatgatagat
      361 tgtacagctg ccccattttc cgcaaaaaaa cgattctgat attggccgca agtctgacga
      421 atggagtgag atgagtatga gcataatgga cttcataaat gatttgaaaa agaaaaatat
      481 caccttatac cataacaagg gaaaaatcaa aatcatcggt cctcaagaat tattgacggc
      541 tgacttaaaa cagcaaatca agcggtataa agatgacatc atcgcagcgc tggaagcggg
      601 cgaaacggac attgagcgaa gctttccgaa agcggcgccg tctaaaagcg gaacctatcc
      661 tttgtcaagg gaacaaaaaa ggatgttcat tttaaatcag cttgatgaca gcaaaacggc
      721 ctataacatg ccgatggcag taaaaataaa cggtgaagtt caaatcagcc gaattgaaca
      781 ggcatggaaa gcgctcatca aaaggcatga atcgttaaga acttcttttg tcatgctcga
      841 tggcgaaccg gtgcagaaaa tcgaacagga agccgagttc cgacttgaat acagcgagtt
      901 gggggatcaa agcatccagg agaaaatcag ccgtttcatc aaaccttttc aattagaaaa
      961 agccccgctt ttaagagcgg aaatcgtaaa agtggatgaa gctgagcata tgatgatcgt
     1021 tgatatgcat catatcattt cagacggcat ctctatcgga atattaatga aggaattcgc
     1081 cgactgctgt gaaggcaaag aattacctcc ggtggcggtt cagtataaag attattcaga
     1141 gtggcagaaa gacatcgagc agcagagccg gttgaagaag caggaagcgt attggcttaa
     1201 caccttccgg ggagacattc cggtattgaa tatgccgctt gattttccga gaccgaaaat
     1261 ccgaagcttt caagggaaca gaacggttgt ggaattggat caggacacca caaaaaaact
     1321 gaagacgata gccgcaaaaa acggcgttac gatgtacatg cttcttttgg caggctacac
     1381 cattctgctg tcaaaataca ccggacaaga ggatatcatt gtcggctcgc cgattgccgg
     1441 aagaccgcat gccgatttaa acgggacaat cgggatgttt gtcggcaccc ttgcgctgag
     1501 aaaccggcca acggggaaca tgacatttag cgaatatgtc caaacggtca aaaacaatac
     1561 attgaacgcc tatgaaaatc aagattatca atttgatgca ttgatcgaac atctgggctt
     1621 gacacaggac atgagcagaa atccgctgtt tgatacgatg tttgatctgc agcatgcgga
     1681 tgatttcgta tctgaagccg gcggggggca ttttgacacg tatgatattc catttcatgt
     1741 cgcgaaattc gatgtaagcc tgacggcatt tttgcatgga gacaacctga aatttgattt
     1801 tcaatattgc acggatctgt ataaaaaaga aacgattgaa agaatggccg gacatttttt
     1861 gaatgtttta aaagacgctg ctcatcaccc tgaactggcg ttaagcgaga ttaggatgat
     1921 gtcggaagag gagaaagaca tcatcttaca tacattcaat catgagaaga cagatggtcc
     1981 gaaaaacaag acactcagcc ggctgtttga agagcgggct gaaaaaacac ccgatcaccc
     2041 cgctgttata ttcgaagatc agcaattgac atatagagag ctgaacgaaa aagccaacca
     2101 attggcctgg cttttaagag agaagggcgt aaagccggat acaattgtcg cgatcatgac
     2161 agaccgttca ctcgagatga tcatcggaat tatcggaatt ttaaaagccg gcggagccta
     2221 tctgccaatt gatccggatt atccggagga cagagtcaaa tatatgctgg aagacagcgg
     2281 ggctgatatg gtcgtcatcc aggagccctt taaatcgaaa atagacgggc gtcaactgat
     2341 aaccgccgaa gacacccgat ctttcagcaa agaaaatctc ccaaatgtca ataaggcttc
     2401 tgatttggca tatgtaattt atacttcggg ttcttcaggc aggccgaaag gtgtcatgac
     2461 gactcaccgc aatgtcgttc attatgttga tgcttttacg aaaaggatcc ctttgagcga
     2521 agatgataca gttttgcaag tcgtatcctt ttcatttgat gcattttctg aagaagtcta
     2581 ccctatttta gcttgctcag ggcgcttggt gatcagcaga aaagtaagcg atctgaacat
     2641 tgatgaactg gtgaaaacga tcgggaagca tcgtgtcaca cttgtcagct gctccccgct
     2701 tctattgaat gaaatcgata aaaatcagca cttaaccttt catccgcaga tgaaatttat
     2761 cagtggaggc gacgtattaa aatttgagta cgtcgaaaat atcatcaaag gcgccgacgt
     2821 ttacaattct tacggtccca cggaagcgac agtttgtgca acctattatc aactaagcag
     2881 cgcagaccgc aaaaaaacga gcattcctat cggcaagcct ttaagcaatt ataaagtata
     2941 cattgcagat caatacggcc ggccgcaacc ggttggagta ccgggcgaac tattgatcgg
     3001 cggcgaaggg gtcgcaaggg gatacttgaa tcatgaaaca ttgacaaaag cggcctttgt
     3061 gctggacgaa tcaggcgaga gagtatatcg tacaggcgat ttagccagat ggctgtctga
     3121 cggaaacatc gagtttttag gcagaatcga cagccaagtc aaaatccgcg gctatcggat
     3181 cgaactggaa gaaatcgaac accggttgct gatgaacgac gatattaatg aagccatcgt
     3241 ggtggcgaag gaagatcagg aaaacagcaa atatctatgc gcctacatcg cattcaataa
     3301 caaaaacgca gatattgagc aggttcaaga acgcttggcg aaggaccttc cggagtatat
     3361 gattccgtcc tgctttatca agcttgatca aattccccgc acgatcaatg gaaaggctga
     3421 tcttaaggca ttgccggagc cggatcgagg ggcatttgcc caagcccgct acgaagcccc
     3481 gcgcaatcaa acggaagcct tacttttatc gatctggcag gatatcttgc cagcggaaca
     3541 gatcgggatt aacgaccatt tctttgacat cggcggacac tcgttaaaag ctttttcaat
     3601 ggccgcgaaa attcaagcgg cattaaaagt cgaagtgacg ctaaaggaaa tcttcagcca
     3661 tccgacgatt aaagacatcg cagcttatat cgtacaaaaa caaaaacaag ttcaatccga
     3721 cattctaaaa gcgaaaaaga aagaatacta cccgctttct tccgctcaaa aaaggctgta
     3781 catcctcaat caaatcgaag aaggccaaac cgcctacaat atgccttttg cgatgaaaat
     3841 aagaggtggg ctccaaacga aaaaggccga gaaggccttc aaaacgctaa tcaaaagaca
     3901 tgagagttta agaacatcct ttgtgaccat caatggcgaa cccgttcaga atataaacga
     3961 aggggtcaca tttgaaatga aataccggga gcttgacaat tgcagccttc gggaacggat
     4021 gaatcaattt atcagaccgt ttgacctgga aaaagcccct cttctgagag cggagctggt
     4081 gagggtgaac gcagccgaac acattctcct gcttgatatg caccatatca tttccgatgg
     4141 cgtatcaatc gggatcttga tgaaagaatg ggcggcttta tatgaagaaa aggaacttgc
     4201 accgttaaag atccaatata aagattattc cgagtggcag cgagagtcct gtcaaaaaga
     4261 ccggttgaaa aaacaggaag aatactggct atccgttttt caagacgcca tccctgttct
     4321 gaacatgccg acggactttc caaggccgca aatgcaaagc tatgaaggag atcgaatcgc
     4381 atttgcaatc gaaagagagc tgacggataa gctgaaaaaa acagcgaaag aaaacggagt
     4441 cacgatgtat atgctgctgt tggccgggta tacaatactg ctttcgaaat acaccggaca
     4501 ggaagatatc attgtcggtt cgccgattgc ggggcgaacc cgggaggagt tggaacagac
     4561 ggtcgggatg ttcgtaggca cccttgcgat gagaaaccat ccgaaaggcg gcaggacatt
     4621 cattgagtat ttgcaagatg taaaagaaaa tacgtttaac gcctatgaaa atcaagatta
     4681 tcctttcgat gaactcgtag acaaattaga tttggaacgg gacatcagca gaaatgcctt
     4741 atttgacaca atgtttgata tgcaggcgct ggatgatgca gagcctgata tagagggatt
     4801 gcatgttgaa cccgttgatt tggagtttca gatttcaaaa ttcgatttgt cgctggcagc
     4861 agcagagtcc gcaggcgtca ttacgtttca cctggaattc tgcacaaggc tgtataaaaa
     4921 agaaaccgcc gaaacgctgg cgcagcattt cgtcaatatc ctaagagata tttccgatca
     4981 tccgcagaag ccgttaaacg aaatcagcat gctttcagag gaacaaagga acacagtgct
     5041 gtatcaattt aatgatacga acgccgaaca cccgtcggga attttttctg agctgtttga
     5101 agagcaggcc gaaaaatcac cgaatcatcc ggcggcggtc ttcaaagatc atatgttgac
     5161 ataccgcgaa ttaaatgaaa aagcgaatca attggccaga acattgcgga aaaaaggggt
     5221 tcagcgggag tcagtagtcg gaatcatggc ggagcgttcg ttggaaatgg tgacaggcat
     5281 attggcggtt ctaaaggccg gcggggcgta catgccgatc gatccggggc ttccgaaaga
     5341 gcgcatccaa tatcttatca cggatagcgg agctgatcta ttgctgaccc agcatcagct
     5401 tattggcagc atcagctttg ccggagaaat catccaaatc gatcaggccg atgcttacga
     5461 cacggatgga agcaatttgg agcatcttaa ttcccccggt gatttggcat acgtcatcta
     5521 tacctcgggt acgacgggaa acccgaaagg cgtgatggtc gagcaccgca atatcattca
     5581 tgcccactat acttggagaa agcactatga attggcatca ttttcagtta acctgctcca
     5641 gcttgccagc atgtcgtttg atgtattcgc aggagattta tgccgcagtc ttctcaacgg
     5701 cggaacaatg tatatggtcc cggatgatgt caaactggaa atgaatctgc tgtatgacat
     5761 gatcaacaaa tacggcattc atatgttgga atcgacgcca agcctgatca ttcctttgat
     5821 gaaatatatc gatcatcaca agcttgacct ttcaagcatg aagctgttga tcatgggttc
     5881 tgacacttgc acgatcaaag attataagtg gctcgttgaa cggttcggac aaaggatgag
     5941 aatcatcaac agctatggcg tcacagaagc ttctgtcgat tccggctatt atgaagaagc
     6001 tttggatcgg attccagaga ttgcgaatac gccgatagga aagccgctgg acaatacagc
     6061 attttatata ttggattcct cattaaaccc gcagccggtc ggcgtttatg gtgagttgta
     6121 catcggcggt gaaggggtcg cgaggggata cttaaacaaa ccggaactca cgaaggaaag
     6181 gtttgttcca aatcgttttg ccgccggggg gaagatgtat aaaacgggag acttggcaag
     6241 gtggctgcct gatggaaatg tcgaattcct cgggagaatc gatcaccaag tcaagattcg
     6301 cggtttcaga atagaaacag gcgaaattga aacgaagctt ttggaaaatc aaaacatcag
     6361 cgaagctgtc gttatagacc gggaagacaa gaaaggacat aagtacctgt gcgcctatat
     6421 tgtcgctcga gcgaaaacaa acgcaaatga actgagggaa tatttgtccg atcatttacc
     6481 ggattatatg ctcccttcct attttattca aatcaacaag atgcccctca cgccaaacgg
     6541 aaagatcgat cgaaaagcgc ttcctgaacc tgccggagat gccatagcgg caaacggata
     6601 taaagcgccg cgcaatgaaa cggaagaaaa gctggccgct gtttggcagg aggtgctgga
     6661 cagggacaaa atcggaatca acgataattt ttttgaaatc gggggagatt caattaaggc
     6721 tctgcaaatc gtttcaaaat tatcaagagc cgatctcaag cttcaggtta aagatctgtt
     6781 taccaatccg tccatcagac acctcagtaa atatgtcaaa aaagaaacga aagctcgcac
     6841 cagcgaaatc gtgcaagggc aagtgccgct gactcctgtg caaaggtcgt tttttgaagc
     6901 gaatcaaagg gagcaaaacc attataatca agcgtttatg ctctacaggg aaaacgggtt
     6961 tgctgaaagg atcgtcgaaa aggttatccg taaactcaca gaacaccatg atgcattacg
     7021 gatggtctat cgggagaaga acggtgaaat cattcagcat aaccgcggct tggaagacag
     7081 cgtcttcgat ctgaatgtct atgatttgaa aacggaaaaa aatctcgagg aaactgttta
     7141 tcagatagcg acgaatattc aaaaagacat atccatcagc gagggcaaaa tgatcaagct
     7201 ctgcgtgttt aaaacaacgg agggagacca tctgctgatt gcgatccatc accttttggt
     7261 agacggcgtg tcctggagaa tattgttcga ggattttgaa gcggcatacg gtcaagcctt
     7321 gcaaggcaaa ccgatagagc tcggttataa aacggattcg tacaaaacgt tttccgaaaa
     7381 actggctgag tacgcaagca gcaaaaagct cctgaaagaa caagaatatt ggcgggagat
     7441 atccaaagga aagatggcat tcctccctaa acatcgccaa gcggcacatg ataactatga
     7501 aaatagcaga acactcagaa tatccctcag ccaaatggag acggaacagc tcctgaaaaa
     7561 tgcgcataaa gcatacaata cgcagattaa tgatctcctt ttaacggctt tgttgatcgc
     7621 ctcaaggcag ctgacggggg aaaacaggct gaaaattctt atggaaggcc atggaagaga
     7681 tgatattttg caagatgtgg atattacgag aacggtcggc tggttcaccg caatgtatcc
     7741 ggtattcatc gacttagaag atgaaacaga cttgtctgtg atgattaaga tcgtgaaaga
     7801 aactttgcgg aaaataccga ataatggaat cggatacggc atcctcaagt atttgagaaa
     7861 agacgaaggg cttataaagg atgaaaagcc tccgatctta ttcaattatc ttggtgagct
     7921 cgatcatgat ctgacgactg aacaattttc atcgtcaaag ctttcaacag gtcaaagcat
     7981 cggagaaaaa agtgcaagag atgcttctgt tgaaatcgat tcagttgttg tcggcaggca
     8041 attgatgatc agtacgacat ttaacgaata cgagtattct ccagatacga tcagcgaatt
     8101 gaatcaagca ttcaaagaaa gtttgcaaat ggtcatcagc cattgtacgg gcaaaaatga
     8161 aaccgaaaaa acgtcctctg actacggtta tgacaaactc tcgttggaag atcttgaaga
     8221 attattaaat gaatatgaat ctatcgacag ctagatatca tgaaaaaagc taaagaagcc
     8281 cccttgaaat ggttaacctg ctgcttaaaa atgggcggcc catgtggatc tgaaaacaaa
     8341 aggaattcta ggagatgaga tgacgatatg aaaacaaaag ttgagaaaat atatccgtta
     8401 tcaaacatgc aaaaaggaat gctgtttcat gccatgaaag acgaagcgtc acacgcatat
     8461 tttgagcagt tcatcattga attaaaaggt gatgtggatg agcggatgtt tgaagaaagc
     8521 ctaaatgaag tcatgaaaag gcatgagatc ctaagagcat catttcatca cagattggat
     8581 gagccgctgc acgtcattat caaagatcgg cacatgaagt ttgactatct tgatatacga
     8641 ggccggcatg agcaagacgg cgttttggag agttatttgg cagaagatag acaaaagggt
     8701 tttaacttag ccaaagatac gttgatgaga gcttgtctca tcagaacgtc tgacgattcg
     8761 tatcaatttg tctggacgta tcatcacatc ttgcttgacg gatggtgcct tggcatcatc
     8821 ctggatgagc tgttgacgat atatgaaatg aagcggaaag gccaacatca tcaattggaa
     8881 gacccgaggc cgtacagcga ttatatcaaa tggctggagg atcaggataa agaagaagct
     8941 cagagctatt gggagagcta tttgagcggc tatgatcaaa aaaattcgct tccaaagctt
     9001 cgcacacctt ctgagacggg ttttaaaaga agagagaaaa cgattgaatg ttccaaagaa
     9061 ttgacaaatc gcctcatcaa attggccaac cagaatcatg taacgatcaa taccgtgttg
     9121 caaagcattt ggggcgtgat tttggcgaaa tacaacaaca gcgaggatgt ggtgttcgga
     9181 accgttgttt ccggcagaga tgccgaagtg gaaggtatag aaacaatggt cggcgtcttc
     9241 atcaacacca tcccgaccag aatcaggctg ggcaaagaca agctttttaa agacgttctg
     9301 cgccaaaccc aagccgacgc tttggaaagc agccgctaca attacatgaa tttagcggag
     9361 gttcaagcgc taagcgagtt gaaaaatgat ttgatcgacc atgtcatggt ctttgaaaac
     9421 tatgccgttg accaaaaagc gtttgaagaa aaaaatgatg taggttttga aatggtgaat
     9481 gtcagcgggg aagaacagac caattatcat ttcagcattt ccgcggctct tgacgatcaa
     9541 ttaaagctgc tcttcatcta tgatgaaaat gtatatgacg acaccatcat cgaaacatta
     9601 gaaaagcata tcatcaccgt cgcagaacaa gtggcggaag atgaggcaca aacattacaa
     9661 gacatcaacc ttgtgtcaaa agaagaacaa cagaggattt tagacacatt caacgataca
     9721 aaaacggatt atccgaaaga caagccgctt catgagctct ttgaggaaca ggccatgaag
     9781 acgcctgacc agacggctct tgtgttcggt gctcagcgca tgacctatag agagctgaat
     9841 aagaaagcaa accaaaccgc gagactcctc agggaaaaag ggatcggcag aggctcgatt
     9901 gccgcgatca tcgcggaccg ctcctttgag atgatcatcg gcatcatcgg tattttaaaa
     9961 gcgggaggca cgtatttgcc gatcgatcct gaaacgccta gggacagaat cgattatatg
    10021 ttggaaaata gcggtgcggc attgt
//
