LOCUS       NZ_NKAX01000023        10348 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus cereus strain 32-6 32-6_R1_(paired)_contig_23, whole genome
            shotgun sequence.
ACCESSION   NZ_NKAX01000023
VERSION     NZ_NKAX01000023.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 235225)
  AUTHORS   Cui,Y.
  TITLE     Improper usage of probiotics constitutes a potential risk of public
            health
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 235225)
  AUTHORS   Cui,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2017) Beijing Advanced Innovation Center for Food
            Nutrition and Human Health, College of Veterinary Medicine, China
            Agricultural University, Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 9.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009935145.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:19:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,979
            CDSs (total)                      :: 5,895
            Genes (coding)                    :: 5,727
            CDSs (with protein)               :: 5,727
            Genes (RNA)                       :: 84
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 76
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 62 of 168
            Pseudo Genes (incomplete)         :: 100 of 168
            Pseudo Genes (internal stop)      :: 56 of 168
            Pseudo Genes (multiple problems)  :: 40 of 168
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:31:34
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3683
            Orig. end                         :: 14031
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NKAX01000023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10348
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10348
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10348
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5348](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5348)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(58..1266)
                     /locus_tag="CE086_RS25060"
     CDS             complement(58..1266)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197707.1"
                     /locus_tag="CE086_RS25060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AimR family lysis-lysogeny pheromone receptor"
                     /protein_id="WP_089607485.1"
                     /transl_table=11
                     /translation="MQVLLDFNDMQESLKLSGYTNRKLAMRFKVTHTTVNSYFNKKGKF
                     DFMHFVDALRLYKPKDIKFRRNCIKECIPTLSHKNLKLALEVLDMFGEYELQDMIMQQI
                     NKFKSNQTETEKEMKKGISKTVRINLNLVPLYQTLRERSENTITPKVFFEKVDKMRKNQ
                     KYIDNELVIMLILNTIYSFFDLGNYKMVNEYIQQLLPDIIKIKCRSLRDSFLLRIKEME
                     IFVNLHENKLRESRQQCFDIINDETNCYVSTKAVAYCKIGESYVFSDYQKAKEYMEKSL
                     KVIGDPHNKKLELRREKVLNTLLFLRIYHKRDLHTINFDDLDEAERAFLYVRLGENEKA
                     IRILQTLKSNNGYLSSFQLYYLGLAVGGKEGKRYLELSLESFSKSGDFFYMFLPKEALK
                     CYN"
     misc_feature    1584..1598
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.24"
                     /tool="antismash"
     gene            1871..2980
                     /locus_tag="CE086_RS25065"
     CDS             1871..2980
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675870.1"
                     /locus_tag="CE086_RS25065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_119683431.1"
                     /transl_table=11
                     /translation="MKGHIRKRGNKYCIVIDIGPDPETGKRRQKWFSGYKTKKEAQADV
                     AKKITELNEGTFIEPSKVTLKDYLNHWLEIKSMSIEKSTFAGYKAFINQHVIPSIGMVA
                     LHKLNVIHIQKCYKTAIDKGIANNSILLMHRILKSALNLAVKQNIISRNPADFAEIPKK
                     EKTPIQTWTEEEVKKFLAHSQESRYHIGYLLAITTGMRLGEVLGLRWQDIDFEKHTVTI
                     NQTSGHDNKIKKTAKTNSSKRTIPVPNETIAALKKHKILINKEKLKFGSAYLDQDLINC
                     NEFGRIIKRAHFRKSFIRMTHKVGIKEIKFHDLRHTHATLLLKQGVNPKIISERLGHTD
                     ISMTLSVYSHVLPNMQEEAVKNFGKSIFG"
     gene            complement(3019..3720)
                     /locus_tag="CE086_RS25070"
     CDS             complement(3019..3720)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736205.1"
                     /locus_tag="CE086_RS25070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF3962 domain-containing protein"
                     /protein_id="WP_161520418.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIRQEQLDYIFQSWYEIIHDWK
                     PNRLVEPPKYEWQSDLISNLPILHDNETYSKWVPALISHIFCERPIYLENTNAEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYKMI
                     GQTNLDMTTFV"
     misc_feature    complement(3712..3725)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            complement(4032..4517)
                     /locus_tag="CE086_RS25075"
     CDS             complement(4032..4517)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="CE086_RS25075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_003270601.1"
                     /transl_table=11
                     /translation="MNMIELKSIIHSYKLKRKIAKDLYGKRDELTLLLNEFNKMKCTVT
                     SEKKKNNILSRLQLIYQNMKLDKQYPLSVAFDSKLLERLEKEPLHTIEDGVTCLHLMLD
                     ENYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGVVVGLLGAISFGGLLLSICSIT"
     misc_feature    4549..4567
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     gene            complement(4727..>4861)
                     /locus_tag="CE086_RS29525"
                     /pseudo=""
     CDS             complement(4727..>4861)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="CE086_RS29525"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="YMYLPTKNIAIINGYIQSFK"
     gene            complement(5001..5348)
                     /locus_tag="CE086_RS25080"
     CDS             complement(5001..5348)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016512777.1"
                     /locus_tag="CE086_RS25080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_161520419.1"
                     /sec_met_domain="TIGR03601 (E-value: 1.5e-43, bitscore:
                     140.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQALSLNDYRSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGCGGGRCGGFRCGGFRCIGCFGCFGCGGCGGCGGCGGCSNCFDGFSGTTDTTDTT
                     GTTGTIITYEY"
     gene            complement(5897..6160)
                     /locus_tag="CE086_RS25085"
     CDS             complement(5897..6160)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="CE086_RS25085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF3937 domain-containing protein"
                     /protein_id="WP_002082716.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            complement(6558..7247)
                     /gene="tenA"
                     /locus_tag="CE086_RS25090"
     CDS             complement(6558..7247)
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="CE086_RS25090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thiaminase II"
                     /protein_id="WP_023522051.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGEHLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAEKVTEADRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDK
                     DVKDVIG"
     regulatory      complement(7375..7480)
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    7519..7531
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            complement(7554..8468)
                     /locus_tag="CE086_RS25095"
     CDS             complement(7554..8468)
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="CE086_RS25095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_003270605.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 3.2e-23,
                     bitscore: 76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 4.8e-15, bitscore:
                     49.7, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 4.8e-10, bitscore: 33.0,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 1.7e-54,
                     bitscore: 178.1, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 4.8e-07, bitscore:
                     23.2, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLNLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRNEEGF"
     gene            complement(8473..9921)
                     /locus_tag="CE086_RS25100"
     CDS             complement(8473..9921)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     5.7e-30. Score: 96.6. Matches aSDomain:
                     nrpspksdomains_CE086_RS25100_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="CE086_RS25100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471611.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.1e-29, bitscore:
                     96.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMHEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYNVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNENWS"
     aSDomain        complement(8515..9486)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CE086_RS25100_Aminotran_1_2.1"
                     /evalue="5.70E-30"
                     /label="CE086_RS25100_Aminotran_1_2.1"
                     /locus_tag="CE086_RS25100"
                     /protein_end="469"
                     /protein_start="145"
                     /score="96.6"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMHEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYNVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     misc_feature    complement(9997..10011)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
ORIGIN
        1 ataacactac ttttacaaac ttatttttca agtgcttcac caccttttat gtatagttta
       61 attataacat ttcaatgctt cttttggtag aaacatatag aagaaatcac ctgatttaga
      121 aaaactttca agggatagtt ctaagtatct tttcccttct ttgcctccaa ctgcaagccc
      181 caaataatac aattgaaaac tactaagata accgttgtta ctttttaagg tctgtaaaat
      241 tctaattgct ttttcattct cacctaacct tacatataaa aaggctctct cggcttcatc
      301 taaatcatca aagttaatag tatgtaaatc tctcttatgg tatatcctta aaaacaaaag
      361 tgtattaagt actttttctc tccttaattc tagtttctta ttatgtggat caccaataac
      421 ttttagtgac ttttccatgt actctttagc tttctgataa tcggagaaaa cataactttc
      481 tccgatttta caatacgcta ccgccttagt actgacataa caatttgtct cgtcgtttat
      541 tatatcaaaa cattgttgac gagattctct taacttattt tcatgaagat tcacaaaaat
      601 ctccatttct tttattctta acaaaaatga atctcttaaa gagcgacatt taatttttat
      661 aatatcaggt aataattgtt gaatatattc attcaccatc ttgtaattac ctaaatcaaa
      721 aaaagaatat attgtgttta gaatcaacat tattacaagt tcattatcaa tatatttttg
      781 attttttctc attttatcaa ctttctcaaa gaacacctta ggtgtaatcg tattttcact
      841 tctttctcgc aatgtttgat ataaaggaac caagtttaag tttattcgta ctgttttcga
      901 aatacctttt ttcatttctt tttcggtctc tgtttggttg ctcttaaatt tatttatctg
      961 ttgcataatc atatcttgaa gttcatattc tccaaacata tctaacactt ctaatgccaa
     1021 ttttaaattt ttgtgtgaaa gtgttggtat gcactctttg atgcagtttc ttctaaactt
     1081 aatatcttta ggcttatata acctaagtgc atccacaaag tgcataaaat caaatttacc
     1141 ctttttatta aaataactat ttacagttgt gtgtgttact ttaaaacgca tcgctaactt
     1201 cctatttgta taaccggata attttagact ttcttgcata tcgttaaaat ccaataaaac
     1261 ttgcacaatc tttgtcctcc ttatggacaa aagacacgtc atccctagtt tttacattta
     1321 cagagaaacg tgtcattata tctaagttgt gtgttatact aatgtacgaa gacttatgac
     1381 agttgttttc cctactgtgg ttagggagac ggtgtagggg tgttgcagca ccacttacac
     1441 agtcatgggt cttttttatg tccatttttt agttattttc ataatatcac aaaaattagg
     1501 aattttattt atttgctaat ctgaaaatta ttgagaaagt ttaataatta atataacaaa
     1561 gtattctctt atattggata acaagaatat ctcttccact cattaaactt aatatatata
     1621 atggttagct agtattttaa ctaccccatc acatgtacta ttgcagctat ttatcaaacg
     1681 cttttaattt tatttccatg aatacagcaa aaaaagaggc cctatggctc ttttttttat
     1741 tccttaataa taatctaaat atggtaaaat atacccatcg ctgatatgtc caaacatgta
     1801 attttcatag cagcaaaatt acaactagac ttatacaaca tgattcaaaa caaatgaagg
     1861 agtgttttaa gtgaaaggac atattcgaaa aagaggaaat aaatactgta tcgttattga
     1921 tatcggtcct gatccagaga caggaaaaag aagacaaaag tggttttctg ggtataagac
     1981 aaaaaaagaa gcacaggctg atgtggcaaa gaaaattaca gagttgaatg aaggaacttt
     2041 tatagagcca tctaaagtta cactaaaaga ttacctaaat cattggctag aaattaaaag
     2101 tatgagcata gaaaagagta cctttgctgg ctataaggca tttatcaacc aacatgttat
     2161 acctagtata ggaatggttg cgctccataa attaaatgtt atacacattc aaaaatgtta
     2221 taagactgcg atagataaag ggattgcaaa caattctatt ctgcttatgc atagaatttt
     2281 aaagagcgct ttaaacctag ccgtaaaaca aaatattatt tctcgaaatc cagcagattt
     2341 tgctgagata cctaaaaaag aaaaaacccc tatccagact tggacagagg aagaagtaaa
     2401 aaagttttta gctcattcac aagaatcacg atatcacatt gggtatctac ttgcaataac
     2461 tacaggtatg cgtctgggag aagttctagg gttacgatgg caggacattg attttgaaaa
     2521 acatactgtt acaataaatc aaacatctgg tcatgacaat aaaatcaaaa aaactgcaaa
     2581 aacaaattca tcaaaacgta caattcctgt acctaatgaa acaatagcag ccttaaaaaa
     2641 acataaaatt ttaatcaata aagagaaatt aaagtttggt tctgcttatc tagatcaaga
     2701 tttaataaat tgtaatgagt ttggaagaat cataaaaaga gcacatttca gaaaaagttt
     2761 cattaggatg acacacaaag taggtataaa agaaattaaa ttccatgatt taagacatac
     2821 acacgccact ctactattga aacaaggagt taaccctaaa atcatcagtg agcgattagg
     2881 tcatacagat atttcaatga cattaagtgt ctattctcat gttttaccga atatgcagga
     2941 agaagcagtt aaaaacttcg gtaaaagtat ctttggataa cctatgtttg caaaatgttt
     3001 gcattttatc aaaaaaagtc aaacaaacgt tgtcatatca aggtttgttt gacctatcat
     3061 cttatattct tgataaaatc tccgaattcc tattgaaata tttaataatg gagcgttctc
     3121 accaccgcgg gttatgcatt cgaatcgata tacataggag aaaaaatctt gtgttttttc
     3181 atcttttatc ggccctgaca tcgcctcaca aatattttgt gatctaagag gggaaaaata
     3241 gatttcttct gcatttgtat tttctaaata tataggacgc tcacaaaaaa tatgtgagat
     3301 taaagcgggc acccacttag aatacgtttc attatcatgt agtattggca aattagaaat
     3361 caaatcggat tgccattcat attttggtgg ttctactaat ctatttggtt tccaatcatg
     3421 aataatttca taccaacttt gaaaaatata atcgagctgc tcttgtctaa taggttcttt
     3481 cgatacaatc catggtgtat tttcatttaa tacgtacggg ttatgctgaa taaacaatat
     3541 atccgaaaac atatcataca atctttcatt caaacgtttc aacttactcg ttaataaaaa
     3601 tgtcttataa tgtatctcta cgatgtccag ccattcaata ggaaagtata taaatgatac
     3661 cttttcattt aaaaggggtt ctactatatt ttcaaatgtt agcagcctta attttttcat
     3721 aaaatgattc cttcctttct tcagttgtct tgattatcaa aaagctagaa caatagttac
     3781 ttaatctaat ccatttaaac caagtattta aaagaaacgt aaaaactgtt tatctacata
     3841 agttattatc aaaccgactc ttttaaaaat tcaaagtaat tatcatgtat aacacctcta
     3901 ttatatttgt atttcaatac attcgaatta tacattacat ctaatgcatt gtacttatca
     3961 tttacaataa ctttacaaaa aaacaaaaaa agaacacctt aattcggtgc cctttttaca
     4021 aatatcaacc tttatgtaat tgaacatata gataacagta atcccccaaa tgaaattgct
     4081 cctagtaaac ctactacaac tcctgttaaa caaatacaat cagcaagaca aatagaagac
     4141 tgcgataatg gaacaaatga cctacttgta ctcgaaccat attgttttat tttctcataa
     4201 ttctcatcta acattagatg taaacatgtt acaccatcct caatagtatg tagaggttct
     4261 ttttctaatc gctccaataa tttactatca aaagcaactg atagaggata ctgcttatct
     4321 aatttcatat tttgataaat tagttgcaaa cgagataata tattgttttt cttcttttca
     4381 gatgttactg tacatttcat tttattaaat tcatttaata acagtgtcaa ttcatctctt
     4441 tttccgtata aatctttagc tatttttctc tttagtttat acgaatggat aatagatttt
     4501 agttcaatca tattcataca cttcctaatt acatattatt tacccgatta ttttatagat
     4561 aaacagtact ttttaatctt atttaaatta cactcaagca tcttcattta ctatcgatta
     4621 acattacatt aaccttacaa agttgtaata aattcattag ctagaagaaa tcaatcccta
     4681 agctagacag atatttgatg ataaaaccat aagaaaagag caatgattag attttaaatc
     4741 tagtcattgc tttatccatc acctcttgat tacatctata tatcttattg ttactctttc
     4801 acttgaatga ttgaatatat ccattaataa tggctatatt ctttgtcggc aagtacatat
     4861 ataaaactaa tgttttttaa ttgataaagt gaagctttaa ttagccttca ccaatcgggc
     4921 ttttatgggt agtccgccac tttgctctcg ctgaattttt ttagggtctt actgcccggc
     4981 aaatagcagg ataaatgcaa tcaatattca tacgttataa tagtaccagt agtaccagta
     5041 gtatcagtag tatcagtagt accactaaaa ccatcaaaac agttagagca accaccacag
     5101 cctccacaac ctccacaacc tccgcaacca aaacaaccga agcaaccaat acaacggaat
     5161 ccaccacaac gaaatccgcc acaacgacct ccaccacaac caccacaacg accacaaccc
     5221 ccacagcgac gagcagcatc ttcaatatag taatatggat attgattttg ctggtcccaa
     5281 tagacaatat ttccagatcg gtaatcatta agacttaacg cttgtagttc ttgttgaaac
     5341 tgattcattt tcataacctc cgtttataaa atacaacctc attgatgctt ccctatattt
     5401 ctgttcgtta catctaagta aaaatagcgc tataaactaa gttcaatacg tacaccaaca
     5461 aagtatgact tatcactaaa tgatgcacct tgttcatata cctatttttg ctatgggctc
     5521 atttttataa agtgaaactt taatcagtgg gggggagttc atcccccgct gattattagc
     5581 cctcaccaat caggctttta cgaacagccc gttaatgcgg gataaataaa aattaaaaaa
     5641 tatgaattct agcgtcatat cttcatacct actaaattca attaatttct caataattat
     5701 gttgccatat cctatatatt tccgtctgcc gcttaaattt ttctgtaatg taaatgcgtt
     5761 tcgctataac atctcctagt gaccggttta tatgacggat aagaaattcc tacaaagaaa
     5821 aaagccctta ttagggcttt tcattctatt tctccagcaa aactctctat gaaattccta
     5881 atagaaaaag acacacttag attgatgcgt ctttttgtga tgcctctttt gtgaaatcat
     5941 ataaagcaat tgcgcctaaa agaacaagta taccttgagg aacatctagt aaaatagttt
     6001 tccaaatttc cggaattacc catttaatat ctgctgctgt ttcaagatat gtttggaaat
     6061 agctgattgt aaaattaatt atccctaaaa atacaaacaa cgataaacca aatcgaatta
     6121 atttcttatt tgtaaacata taacacctct caaaattcat tggatattcc tgcttttgct
     6181 tctttatatt tacatatctc tatatctaat tttaacacat aaagagctca aaaaaatatg
     6241 atatgcctat ttgcatatcg tattaacaac actccatgat gatttagaaa ttcctgcaaa
     6301 gaaaaaacaa ctccgttaca gctaacagct cgggtttctt ttcttattta acttcatacc
     6361 accaacattt acgttctttg attgtaaaat aaatgtgaca gaagtttatc cagttaatgc
     6421 gtagacaagt aaatgagcta aattatcgat attctatttc gccatatccg tagcactaat
     6481 gactttacgc aaatttgaca agaaaaaata tatagatagg tatgatccat acctatctat
     6541 aacattgctt ctattcctta tccaattacg tcttttacgt ctttatccac tggccacttt
     6601 tctaatgtat aagccatttc ccaaaatgaa tattcatatt gactgctaat aataaaatgc
     6661 tgtttcatac gtttgcggtc tgcctctgtt actttttcag cgatagtatc taaacgtgta
     6721 atttgttctt caactagcgt acggaaccaa tcagatccat aagcagaaat ccattcgtta
     6781 taaattggct cttccggctg gcactctttt aagtgttcgc caatttcata atacaaccaa
     6841 tagcaaggta aaattgctgc aatgatatct cctaaatgcc cttcatacgc agcacgatac
     6901 atatgtgaag tatacgcata tgcagttggc gcaggaataa aattatcttt ttcttctttt
     6961 gttatcccta gctttttagc aaaattctca tgtaaagata actcggcctc atatgtattt
     7021 tgagcatgat gtgccatacg agctgtcgtc tctaattcaa gagcttttgc agctcctaaa
     7081 gtttgtactc tagcaaaatg actcaaataa tatgaatctt gaagcacata atagcgaaaa
     7141 ctagctaaat ctaatgttcc ttcaccaagt ttttttacaa aaggatggtt aaaactcgct
     7201 tcccaaattg aatctacttc tttacgtaat gattgtgaaa aagtcattta aatccctcca
     7261 ataatgtata aaatataagt atttatccac aattattaaa gctgtaaaat aaaaaaactt
     7321 tctttacagg cgtaaagaaa gtgaaattga cagtatttat ttactcatta aatagcaacg
     7381 tctttcccac ttccctacgc tggcattaac cagatcaggt actaagagtc tcaaaatcac
     7441 atttgatctc agcctataaa aggcccccct agtggataaa tattttgttt ttataatgta
     7501 ttcatcatag catacttcgt gaaactgtca aaagaattag ttatataatc tccttaaaac
     7561 ccttcttcat ttctaacttt taatgatgtt tcaataatca tatctaatag tttgctataa
     7621 ctaattcccg ctgcttctgc acttttgggc aataggcttg attgtgtcat ccctggtaat
     7681 gtattaattt ccattacata tggaattccg tccttcacca tcatatcaac tcttgcataa
     7741 acactacatt ttaatgcttt ataacaagcc agtgaagctt tatttacacg ttcattaatt
     7801 tctgctggaa gttcgataac ttcctcaact gtactagtat catcatattt tgcattgtaa
     7861 tcaaagaact cggctgcatg tcgaattgaa actataggta actgttttcc atccagaata
     7921 gaacatgtaa tttcatcacc ttttatatac ttctcaatta ctacttcaga atcccattcg
     7981 aatacagttt ctagcattga gatcaattca tttttattgt aaacgatttt caccccgaca
     8041 ctcgatccgc cggagtttgg ttttaccact aatggaaatc ctaacttatc taactcatca
     8101 agatttagat cctccatttt tgtaagttca atccaatctg gtgtttctac accttcataa
     8161 cgtaaaatct ttttcgaaat atttttatcc atacatatac tactagataa catattactt
     8221 ccgctataag gaatccctaa actctccagt gttccttgaa ctgtcccatc ttctccgtat
     8281 tttccgtgta atgcgagcaa tgcaaagtca atgtctttcg ctttttcaat taaatccatt
     8341 ttttcattta atgtaattgg gactatttca tacttatttt tatctaaatg agcaatcatt
     8401 tcattcccgg tcataattga aacttgtttt tcagacgata ccccgcccat aataacaccg
     8461 attttcattt tctcaactcc aattttcatt ttttaatgta tcaccaatta ttttaattcc
     8521 ccgaactatt tcttcttctt tcaagcgcga aaaccctaat cggaaagtgt tcgctccttc
     8581 tccatcagag taaaaaacat ctccaggtga aaacgtaacg cctttctcat aacacttttg
     8641 taaaagtgtg cgggcgttca tatgctcttc tagctctata aataaatgca gtccaccatc
     8701 tccagtcatt cttttgaaag gaatgtactg gttacatgcc ccgacagcta actcatattt
     8761 tttcttataa acagatcttg ccttttttaa atatttttca aaatatccct catgtaaata
     8821 ttgaaacaga acagcttgat ctagcgtaga tgtgtgaatc gttcttgctc tttttacgct
     8881 ttctaaataa tgaatcagct ctttatctgc aataatccac cctacacgta aaccagggaa
     8941 aagtaccttt gaaaagctac taatataaat cacattattg ccagcaccag caaaagttaa
     9001 taatggagct aaatgtgaac ctgaataacg taattcttca ttaaatccgt cctctataat
     9061 aggaacgttg tattttgaga ataacctcat caattccgtt cttttctctg aactcgtaac
     9121 aataccggtt ggattatgat aagaaggaat taaataagca aaatcaaaat ctttttcacg
     9181 caaacttttt tcaacttcat tcgtatcaat accatcctca tgcatatcaa tcccatgaac
     9241 ttcaagtcca tgtaaacgga aaagcttaag tgcagcatgg tgagttggat tctcacaaat
     9301 gacacgaccg gatttttttg ataaagaaga gagtacaata tccaatcctt ctgtaaatcc
     9361 atttgtaatt agaatatctt tattcgaaat gtctacacct ttcatttcca tgtaatgaag
     9421 taaataattc attaacggtc tatatccctt tgcatatccg tagttcaata cgatgtcccc
     9481 ttcaatggac atacgagtaa gaaaagctct tttgaaattt tcaacatcaa atagcttctc
     9541 atccggggct atactattaa aaacaatcat tcccttttcc caacgaacac catgtttcat
     9601 taaatctaat tcgtccgcta atgcggtcac tgtattaagt ttatttttcc aatctatttc
     9661 aacagacgaa gtgttagata tatccacctt cgcaacaaag ttcccttttc ctttaactgc
     9721 ataaatgagt ccttcttgct ctaaatctgc ataagcagcg agtacagtgt ttctactcac
     9781 acttagtaat tcacttagtt cccttgttga tgggattttt tgatccccga gcaaatgccc
     9841 tttcataatc atctttttca aatagtcttt taattgaata taaacgggac gatctttaac
     9901 aactttaaaa tccttaaaca attccttacc ccctgcaatc atttttgcat atttatttca
     9961 ttctcaaaag gtccacgaca tatgtatttt ttataaaggg cggtacggtc ttacaaatct
    10021 tttcgatata agttaattac atcttttaaa gctacagctg cagcgcctcg tggattagaa
    10081 gccccaccaa ttgtagtaac ttgtcccatc gtatctgtaa agaacgtaat tcctttttcg
    10141 gtcccattta catttgctaa cggatgatct ttttctattt cgcacggttc aacatttaaa
    10201 ttcacattgc caccctcatc cttatatatt gatgctatta atttaataat tttatgttgt
    10261 tccttagcgt tttgtatttg ctgttttgta acgtgctcga ttccttttat atgtatatca
    10321 gtaagtgttt tctctgtttc cattaagc
//
