LOCUS       NZ_NKAX01000002        10267 bp    DNA     linear   CON 03-APR-2024
DEFINITION  Bacillus cereus strain 32-6 32-6_R1_(paired)_contig_2, whole genome
            shotgun sequence.
ACCESSION   NZ_NKAX01000002
VERSION     NZ_NKAX01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 532835)
  AUTHORS   Cui,Y.
  TITLE     Improper usage of probiotics constitutes a potential risk of public
            health
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 532835)
  AUTHORS   Cui,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2017) Beijing Advanced Innovation Center for Food
            Nutrition and Human Health, College of Veterinary Medicine, China
            Agricultural University, Yuanmingyuan West Road, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 9.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 20x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009935145.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:19:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,979
            CDSs (total)                      :: 5,895
            Genes (coding)                    :: 5,727
            CDSs (with protein)               :: 5,727
            Genes (RNA)                       :: 84
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 3 (5S)
            tRNAs                             :: 76
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 62 of 168
            Pseudo Genes (incomplete)         :: 100 of 168
            Pseudo Genes (internal stop)      :: 56 of 168
            Pseudo Genes (multiple problems)  :: 40 of 168
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:31:34
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 493377
            Orig. end                         :: 503644
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NKAX01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10267
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10267
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10267
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5267](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5267)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(941..1384)
                     /locus_tag="CE086_RS04715"
     CDS             complement(941..1384)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="CE086_RS04715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX hydrolase"
                     /protein_id="WP_065483848.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKILMVKNKGENGSYYTLPGGAVKLGETL
                     EEAVIREVKEETGLHINVKGICSISEAFFEERDHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDLLQRKETVPYFFNGTIVHQSS"
     misc_feature    1465..1483
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.4"
                     /tool="antismash"
     gene            complement(1664..2089)
                     /locus_tag="CE086_RS04720"
     CDS             complement(1664..2089)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="CE086_RS04720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_076876064.1"
                     /transl_table=11
                     /translation="MKIVQQWVQEDSDYIREKVIEYNQKYLADEEKTPSEKVSFIVRNE
                     KEEIVGGVTAITFWHHIHVDFLWVSEEYRHEGYGSKLIKLIEEFAIEKECRLINLDTFS
                     FQAPDFYKKHGYKVIGVSEDHPKGHNHYYLEKRLKSI"
     gene            complement(2220..3428)
                     /locus_tag="CE086_RS04725"
     CDS             complement(2220..3428)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="CE086_RS04725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="OFA family MFS transporter"
                     /protein_id="WP_161519867.1"
                     /transl_table=11
                     /translation="MKQSSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDNLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSVISFIISILIRPPNVEKKKELKHLHR
                     KVA"
     gene            complement(3708..3851)
                     /locus_tag="CE086_RS29275"
     CDS             complement(3708..3851)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="CE086_RS29275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000653680.1"
                     /transl_table=11
                     /translation="MKDKVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            complement(3883..4899)
                     /locus_tag="CE086_RS04730"
     CDS             complement(3883..4899)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="CE086_RS04730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_161519868.1"
                     /transl_table=11
                     /translation="MYTYDKLISWVENIKEKNHSSATALCIIKDNKIVLEHYSGYHSNT
                     STTKRVTASSQFNVASARKSYLGLMIAYALYEGKINSIDDEAIKYFKDFDPVLLGKTTI
                     RHLVTHSHGLEETNDGTIFREFEPGQSWAYRDINVRMMTRLIYQLYNKSFPELLKERVF
                     KPANFQETGWRVQQDENLVDVVNNPNEDAISEIGTVDDGTEKNLFVSAREFAQWGNLHL
                     NQGMIDDEQIVPKEVIKIATSLQSPTYINKELPQNGLFWFIQNEPAQLSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVVDTFSNGNRA"
     gene            complement(5001..5267)
                     /locus_tag="CE086_RS04735"
     CDS             complement(5001..5267)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071381.1"
                     /locus_tag="CE086_RS04735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_147801767.1"
                     /sec_met_domain="TIGR03601 (E-value: 1.1e-47, bitscore:
                     155.5, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQHELQTLNLNDYQTGNVVYWDQQQSQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGRCGGCAGRCGGCIGCAGCFSCFNCWNWWII"
     gene            complement(5467..5913)
                     /locus_tag="CE086_RS04740"
                     /pseudo=""
     CDS             complement(5467..5913)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="CE086_RS04740"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="NUDIX domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKSKFHHIVRAVMIKDEKLLVAEYIGQHYFLPGGHIEI"
     gene            complement(5978..6436)
                     /locus_tag="CE086_RS04745"
     CDS             complement(5978..6436)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="CE086_RS04745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_161519869.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALKLQVKGNQLKFVPSVAVSLAKVYIKPDG
                     DNVEYIPFAIYDGDLMVGFVMHAVVIETSDMYWINGFIIDQLQQGNGYGKAALQESIDI
                     IKNTFKSCKEIRLTVHKDNLSAKKLYERYGFQSLGHEYDGEQVYRLFV"
     gene            complement(6513..7158)
                     /locus_tag="CE086_RS04750"
                     /pseudo=""
     CDS             complement(6513..7158)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="CE086_RS04750"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="nucleoside hydrolase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAVYILQLANRQDIPVISGATKPLTEEITTYYPEIHGAEGLGPIHVPENVLSIPIYNF
                     GSIAAILIKHGKDLTISYDNETNV"
     gene            complement(7321..8682)
                     /locus_tag="CE086_RS04755"
     CDS             complement(7321..8682)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763222.1"
                     /locus_tag="CE086_RS04755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_161519870.1"
                     /transl_table=11
                     /translation="MNGKRKIFTCISIVGIGLASFSNSSFAASVTDNSIQNSIPIVNQQ
                     VAAAKEMKPFPQQVNYAGVIKPNHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNNRLNLGDWDSKSSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESEYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYPTAVFVSPFIAASITDSNNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKKTQNLINDGIYEGYDN"
     gene            complement(8821..9735)
                     /locus_tag="CE086_RS04760"
     CDS             complement(8821..9735)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673335.1"
                     /locus_tag="CE086_RS04760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DMT family transporter"
                     /protein_id="WP_161519871.1"
                     /transl_table=11
                     /translation="MHNKKWDLRIICAHAFTILIWGTAFPAIRMGLESYTPEHLTLLRL
                     LIASFILLLFSFIYKLRLPDLKDIPAIFIFGALGFTIYQIALNYGEKTVNAGSASLIIS
                     VTPIVTAILASVFLNEKMKLNGWIGGFMSFAGIALISFSQGDAFQLNSGGLFILLAAIS
                     ESLFFVFQTSYLKKYGFLPFTIYTILSSTVCMLMFLPGVYQKILAAPPEVNLSVIYLGV
                     FPTVLPYIALAYIISRAGASEATSSLYLTPITACFIAWIWLGEVPTLVSIIGGGITILG
                     IVIAHIPVLRKEKHRNLANNQSV"
     misc_feature    9858..9871
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
ORIGIN
        1 aagcgtttga agaaatttta attgtagatt cgtttatgat ttctaagtta acattgttat
       61 tggcccattc acttgctaat aaacttgatc ggtctacacc ttgtaatatc ttttttctat
      121 ctatgttaat aatcgtttta aagtcatttg gaataaggtt agaaatatta ggatatttcc
      181 cttcaattag tcttgaatat aatgtagttg ttccgagcgt aaatataata tgactctctg
      241 aaaggtaaat atatacgaat tccgaattgc tgttcattaa ttttagaagt tcactaatgg
      301 ttgcgcttgg tacaatacaa tttactttta tatctgagga aagtcgcgtt tcacgtatcg
      361 ctagtctatg tgagtcagtt gcagcacaaa ttaacttatt attagataac tctatatgca
      421 ccccagtaag aacaggtcta gattcatttt tcgctaccgc aaagactgtt tgtttaaata
      481 cttcaatcaa ttgctctgtc tctactttga tttctgcatg attgtctata agcggtacat
      541 tcggaaactc atctgcagga aatccattta aacttaatgt gatttcttct gattgtatcg
      601 taattaattg ctcattctta ctttttattg ctatatcact tggcattttc tttattattt
      661 caatgaaata ctttgcaggt acaacaatag ttcctgcctt taaaatagtt gcaatctttt
      721 cgtcttcaat tgaaacagga atgaattttt cgataaaaat attcgagtta cttgcaatta
      781 aagtaattcc agattgatct gcagttattt ttataccaga taatatagga attaaagatt
      841 ttgttgatat cgctttgtta acttctgaaa gtgcttgagt aaattgtttg tgattaacga
      901 tgaactccat gttttcccca ccttacatga gataatattt ttatgaagat tgatgaacga
      961 ttgttccgtt aaaaaagtac ggtactgttt ctttcctttg taataaatct agtaaatgtt
     1021 ctggtatgcg taaatatgga gctgctatat gtaattccat ccaagtaatc tcttcgattt
     1081 cttttggtct tgatatatat gtctccccgc caattatttc gcctagaaaa ttaaagaaaa
     1141 ttgcatgatg atctctttcc tcgaaaaagg cttcgctgat agaacaaatc ccttttacgt
     1201 ttatatgtaa ccctgtttct tcttttactt ctcgaattac tgcttcttcc aatgtttctc
     1261 ctaatttaac tgcgccacct ggtaatgtat aataagaacc attttctcct ttgtttttta
     1321 ccattaagat cttttcgttt gttttatcgt ataaaagtgc atatgtaaca tttacttttt
     1381 tcatatgtac ctcctctatt tttaataaaa ggaattttct gtcttctatt cattttacca
     1441 ttaactcctt ttaatatata taagttagtt aagtcttgcc tttaccagta ttggtggaat
     1501 taataagaat aggctactga tataaaaaaa gaacgtacct taggaagatt atccaaagta
     1561 cgttcttgca gttttagaat aaaaattgat tcaattaata ctgaaattaa taataacctc
     1621 gcccttattg catgtgtatt ccaaatacta atgttaacaa attctatata ctttttagcc
     1681 gtttttctaa ataataatga ttatgtcctt tcggatgatc ttcacttact ccgattactt
     1741 tatatccatg cttcttataa aaatcaggtg cctgaaaact aaaagtatct aaattgatta
     1801 acctgcactc tttttcaatt gcaaactctt caataagctt tattaacttg cttccgtaac
     1861 cttcatgcct atactcttca gaaacccata gaaagtcaac gtgtatatgg tgccaaaaag
     1921 ttattgctgt taccccacct actatttctt ctttttcatt tcttactatg aaactaacct
     1981 tttctgaagg tgttttttct tcatctgcga gatatttttg attatattca attactttct
     2041 ctcttatgta atcactatct tcttgtaccc actgctgtac tattttcatt ttaagtgtca
     2101 cgctcctttt tacttatcta ttcaatttta aaatgatatt gtcctgtctt taatgagttg
     2161 gtaaagaagc aaagttaatg gaataaaaaa gagccctagt atgaggactc tttttaaaat
     2221 taagctactt tccgatgtaa atgttttagt tcctttttct tttctacatt tggtggacgg
     2281 attaatattg aaataatgaa ggaaataaca cttaaaacac caattataat gaaagttggt
     2341 tgaaatcctc caagtatcgc tccaatgaat gatcctgcaa gcgcaccaaa tccgaaacct
     2401 tggtagacaa tcccgtaatt tgtactatgg ttttttaatc caaagaaatc tccgacaata
     2461 gctgggaata tagttatatt accgccgaag caaaaggcga cacttgctac acaagcaaaa
     2521 tagatcccgt aatttagcgg aataaaactt aaagtaaaga ctgacaaacc tataataata
     2581 aacgttgcag agacaatttt cattcgcccg attttatctg ataacgtccc aagaatgatt
     2641 ctacctactg tattaaagat tgcaatcata gcaacggcgt ttgcagcggt tgctgcgcta
     2701 agtccaacaa gttgtacgcc gatatctttt accatactga ttaaatacaa accacccata
     2761 cacgaggtga ataacataaa aaatagcaga tatacttgtt tcgttcgcat catttcacgc
     2821 ggagtatagt cattgtgtaa tgtttcagtt acagcattac ttacaattgc ctcacgtaag
     2881 aagaacgatc cgattaacac taaaagtaac acgataatac cccaatataa gaatgcttgt
     2941 gatacaccaa ggttatcgat aagatttccg tttatatatc taaagattaa gctgcccatt
     3001 ccatacgctg atacagatat acctgaaata agccctttcc gatttggaaa ccatttaatt
     3061 aaattagata gtgatgtaat ataagctgtt ccatccgcat aaccaacaac gacaccagct
     3121 aataaatata atagtggtaa ggaagaaact tgtgaactaa gtattaagcc gagtcctaaa
     3181 acaattcctg cagtagcaat aagtttccga aggcctaatt ttttctgcaa cttggctgca
     3241 aataaagttg agaatgataa agaaaaacta gttatggaga aagttatcgc tactgaatta
     3301 agattccatc caaacttact tacaaggggt tgattaaata aactccatgt ataaattgtt
     3361 ccaaggccaa tttgaacaat gattgtacct agaacaatta gtaacggatt tatagaactt
     3421 tgtttcatac taactgtccc cttcttttat cttgacatga attttcacag ttagtatatc
     3481 catcataaat tacaagatac tgtacggaaa atggtaatta agttcatata cacaaaaaga
     3541 cacttcataa agaagtgtct tttgcaggtt atatcagata tttctaaaga aagctttgtt
     3601 aagataagac attttcaaat gcttatgtat acatgttatc aaataatttc taaaatgtta
     3661 tgagacttaa gacgtatttt tcattcatca tatagtcgga tgaaaaatta atattcgatt
     3721 tcatcaaaat ctttcggctg cggtacagtt tgcatattag ctgcatcttt aggatcgcta
     3781 taaattgcat tttgctctgt atttacatct tttatatttt gctttttatt tttgttcact
     3841 ttatctttca ttttcaatca ctcctttttg ttataggagt aattatgccc tattaccgtt
     3901 actaaatgta tcaacgacta aattactaaa ttcacgtaaa taatataagt aattatcacc
     3961 accgtagtta tatcttttat tatacatttt tgcaacaact acattatatt caggtattac
     4021 taaaatagta ggtccagtaa tccctaatat ttgatacgac ccttttggaa cacgttcacc
     4081 aagttcgctt aattgtgcag gctcattttg gatgaaccaa aacaatccat tttgtggtag
     4141 ctctttgttt atatatgttg gactctgcaa actcgtagcg atttttataa cttcttttgg
     4201 aacaatttgt tcatcatcta tcataccttg attcaaatga agattgcccc actgcgcaaa
     4261 ttctctagct gagacaaata aatttttttc agtaccgtca tctaccgtac caatttcact
     4321 aattgcgtct tcatttggat tattaacaac gtcaactaaa ttttcatctt gctgaactct
     4381 ccaacctgtt tcttgaaaat tagcaggttt aaacacacgc tcctttaaca attcaggaaa
     4441 acttttgtta tatagctgat aaataagacg tgtcatcatt cttacattaa tatctctata
     4501 cgcccatgat tgtcccggtt caaattcacg aaaaattgtc ccgtcattcg tttcttctaa
     4561 tccgtgcgaa tgtgttacta aatgtcttat cgtcgtttta ccaagcaata caggatcaaa
     4621 gtctttaaaa tattttatcg cttcatcatc gatagagttt attttcccct cgtaaagcgc
     4681 atacgctatc attaatccta aataactttt tctagcagaa gcaacgttaa attgtgaaga
     4741 tgctgttact ctcttggttg tagatgtatt tgagtgataa ccactataat gctctagtac
     4801 gattttatta tcttttataa tacaaagtgc tgtagcagaa ctatgatttt tttcttttat
     4861 attctctacc caagaaatca acttatcata tgtatacaaa taaatccccc ttaatacgtt
     4921 cgaaaattac aacatgaaag aaaagtgcaa aatagttttt acacctttct ttcatataaa
     4981 ataacttgga ttttctacac ttaaataatc caccagttcc agcaattaaa gcaactgaaa
     5041 catcctgcgc atcctatgca tccgccgcaa cgacctgcac aacctccaca tctaccgcca
     5101 catccaccgc aacgtccacc acaacctccg catccgccgc aacgacgtgc atcgtcttga
     5161 atgtagtagt atggatattg actttgttgt tgatcccaat acacaacatt accagtttga
     5221 taatcattaa ggtttaatgt ttgtagttca tgttgaaact gattcattac gaacccctcc
     5281 atttataaat gtaaattccg tccaaatatt aaagttaata aattcttgta aacaaagtag
     5341 aaacaagact atagactaaa tgcgtacatc aacaaaatat gaacttacac taggtgatgt
     5401 accttgtttt ttagcctatt ttctttatat tcattcgatg gacgtattta ctataaataa
     5461 agtgatttaa aaattactaa tccaacaatt tagtagttca tcacttaact ttctttctag
     5521 taattccttc actaaaggca ttggcatgat ttcataacga tttaaagctt ctttattata
     5581 atctatccag tgaaatgcta aatgagactc actagatttc ggtgttaagt ccgtatgtaa
     5641 atcttgtgac tctacttcaa aaatgtggtt aatttcatga tgaagcactt ctttatcttg
     5701 ccattgattt tctatgacgc ctaaaaattg ttgtatacta cagtttactc caagttcttc
     5761 tcttaactct cttattaatg cattctctgc tgattctcag atttcaatat ggccaccagg
     5821 taaaaaataa tgttggccaa tgtattcagc aactaataat ttttcatctt ttatcatgac
     5881 tgctcgtaca atatgatgga atttactttt catttgatat tcctcctagt cattcaatct
     5941 atacttgttc aaatttcaca cctttacttc ataataatta aacgaataaa cggtatactt
     6001 gctcaccatc atactcatgt cctaatgatt ggaaaccata tcgttcatat aatttctttg
     6061 cagagagatt atctttatgt acggttaatc ttatttcttt acacgattta aaagtatttt
     6121 ttattatatc aatactttct tgtaatgctg cttttccata accattacct tgctgcaatt
     6181 gatcaataat aaatccgtta atccaataca tatccgatgt ctctattaca acagcatgca
     6241 taacaaaacc aaccataagg tcaccatcgt atatagcgaa tggtatgtac tctacattgt
     6301 caccatctgg ttttatatat actttagcaa gcgaaactgc tacggatgga acaaatttca
     6361 actgatttcc tttcacttgt agctttaatg cttcttccca attttctctc gtaacaacct
     6421 ttaactggac attcaatatt gtaagctccc ttatatagag gatgtatgtt tatattcaac
     6481 gtagtgaaat aattcccttt ttcgactaca acttataaca tgatttttat aaaatcttga
     6541 atgaattttt gttcatctat ttctaatgct atatgggctc catcagatat agagctaggg
     6601 cgaaaatcag caaatgtttg tcctttcgta tctccgtatg tatctacatt tactgtacga
     6661 tgcacaaatt caaaaaaact tggatttact aatccactta ttgcaaccac atcatgtagc
     6721 aaaggtcctt ccatagatgg attaagcttt tggtatgcgg atagatagta atcatacatt
     6781 ggtttcatta tcatatgaaa tcgttaaatc ttttccgtgc tttataagaa tcgcagcaat
     6841 actaccaaaa ttgtatattg gaatagataa tacattttcc ggaacatgga tgggccctaa
     6901 tccttctgct ccgtgaattt caggatagta cgtagtgatt tcttctgtca gtggttttgt
     6961 tgcaccacta atgacaggaa tgtcttgtct atttgctaac tgtaaaatat acactgcatt
     7021 atgtgcagca tgtatatgtt caacatttcc gtatccagtt acaataccta caatttcaat
     7081 ttcaggatgc agtaaaccat acataatggc aaatgagtca tcaatacctg ggtctccgaa
     7141 aaataatact ttttccaaga aatactctcc cctcataaaa acatagagtc atcaataact
     7201 atgtatatga aaaaagcaag tatatctatg cgaattttag aatggataat gttttacaaa
     7261 atattaaaaa ggaactagcg tatgctaatt cctttcttca gtaaccattc tcaaatttaa
     7321 ttaattatcg tatccttcat aaattccatc atttattaga ttttgtgttt ttttatcatc
     7381 aggtacaggc ttccaccagt ttcctgtaat aaataacata gttaataaat tataactatc
     7441 gctaaaataa ctttctctct tattcttcat ccaatcccaa ccgctattta cccacttttg
     7501 attattgcta tccgttatac ttgcagcaat aaatggtgaa acgaatacag cagttggata
     7561 actcccaata ttggatccgt ttaattgata accatccaca attttagaag gatttccatt
     7621 cgttttattt tgaatccatg aagagacttt atcagaaatg actttacttc ttttctcgcc
     7681 gtacatcgca tagtccatta caattctcaa tggtacccga ctagcattgt aataataggc
     7741 atttgtatat tctgactcat ctaagaagtc tttaggtgcg ggttgtggtg ggttttttac
     7801 aacgaaatct gaaataagtc ctgtatttgg agagtactta ttactaaatt gcgtataaac
     7861 atcgtacaaa ttattaataa cagtaagcca agttttatca cctgtaaatt cataaaatgc
     7921 tctaaggtgt gacatcatcc aatcagatgg tctcgtatca agtgaacttt tagaatccca
     7981 atctcctaaa tttagtcggt tattatttgt aacattacta gctttaatac cttttgtaat
     8041 catgtcttgt gcttctttca aataattaac tgttccatta gatccccact gcttatgagc
     8101 aagaagaaga gaatacgcaa tatctaaatc cccatcagta gcagaatcaa aatgaccttg
     8161 tgctttttta ctatctgcga caacccatcc cattaaatta ggattttgag agcttttaaa
     8221 agttcttgct gttttaaata aaccgtcata gattttttga gcattcgaat cataaccagc
     8281 cattaatact gtaattatca tcccataccc ttgaccttct gaagttccaa gtggcttaaa
     8341 cccatccgca tcacctgtaa tctctccttt tacgtaataa ccaccaggta aagaagataa
     8401 atcatttttc aaatatttct ttttccaatt atcgtagtaa cttcttacag aagcatttaa
     8461 actttcctgt gtaacatgat tcggttttat aacacctgca taattaactt gctggggaaa
     8521 tggtttcatt tcctttgcag cagctacttg ttgattaact atgggaatag aattttgtat
     8581 tgaattgtcc gttacacttg ctgcgaaact agaattagaa aaactagcta gtccgattcc
     8641 tacaatagaa atacatgtga aaatttttct ttttccattc attatgttgt cagctccttt
     8701 taaaatgctt actacataaa atgtaacata caatatttaa ctgaaataca aaatattata
     8761 tttatcaaca taagaaagaa actatataaa aaagtatcat aaaatcatta ttgaattaca
     8821 ttatacggat tgattatttg ctaaattcct atgtttttct tttcttaata ctggtatatg
     8881 agcaatcaca attccaagta tagtaattcc tccgccaatt atcgaaacta aggttggcac
     8941 ttctcctaac catatccaag caatgaaaca cgcagttatt ggcgttaaat atagagaact
     9001 tgttgcttca gaagcaccag cacgagatat aatataggcc aatgctatat acggaagtac
     9061 tgttggaaag acacccaaat atataacgct caagttaact tcaggaggag ctgctagtat
     9121 tttttgataa actccgggta agaacataag catgcataca gtactagaca aaattgtata
     9181 tatggtaaat ggtaagaagc cgtatttttt taagtaagat gtttggaaaa cgaaaaatag
     9241 actttctgaa atcgccgcta ataatataaa taatcccccg ctattcaatt gaaaagcgtc
     9301 gccttggcta aatgaaatta aggcaattcc tgcaaaactc atgaaaccac cgatccagcc
     9361 atttaatttc attttttcat tcaaaaaaac agaagcaaga attgccgtca ctattggagt
     9421 gactgaaatt attaaacttg cagatccagc atttactgtt ttttcgccgt agtttaatgc
     9481 gatttgataa atagtaaatc ctagagcacc aaatataaaa attgctggga tatcctttaa
     9541 atccggtagt cgtagtttgt atataaatga aaataacaaa agtataaatg aggcgattaa
     9601 taagcgtagt aaagtaaggt gttcaggcgt ataagattca agccccatac gaattgctgg
     9661 aaaagcagtt ccccatataa gaattgtaaa agcgtgagca catataatgc gtaaatccca
     9721 ttttttatta tgcatatttt ctctccttcc ctaaaaaacc agccattttt aaattaccaa
     9781 ttatttcttt tcatcatatt taatagttga tacaatgtct tcaaccagtt atttatcgga
     9841 aaaaccaacc actttgaatt ttttaaataa acgaaggagt tttttatatg acaatcgata
     9901 aaaaagtgcc caaatatcgg cagataatag actatatgaa agaaaaaatt gaaaacggag
     9961 aatggccaat tggaagtaaa atccctagtc agcggcagtt agcaaaatta tttcatgtaa
    10021 atcgaagtac tgtaattact gcgctagatg aacttatggc agatggttta attcaaggac
    10081 aaatcggagc aggaacaatt ataatgaata atacatggtc attattagca actaatcctc
    10141 cgcccgattg gagtgaatat gtaaaagccg gtattcataa accaagtaag ttaatggttc
    10201 gagaaataaa tgaagcagaa gcaaataaaa agcttatcca tcttagtaaa ggtgaacctg
    10261 cacctca
//
