LOCUS       NZ_RZIR01000001        10345 bp    DNA     linear   CON 17-MAR-2024
DEFINITION  Bacillus sp. AIIW2 Scaffold_1, whole genome shotgun sequence.
ACCESSION   NZ_RZIR01000001
VERSION     NZ_RZIR01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. AIIW2
  ORGANISM  Bacillus sp. AIIW2
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 4396332)
  AUTHORS   Kumari,A., Chaudhary,D.R. and Jha,B.
  TITLE     Plastic degrading bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4396332)
  AUTHORS   Kumari,A., Chaudhary,D.R. and Jha,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2018) Biotechnology and Phycology, CSIR Central
            Salt and Marine Chemical Research Institute, G B Marg, Waghawadi
            Road, Bhavnagar, Gujarat 364002, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 08-JUN-2018
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 99.95x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009932115.1-RS_2024_03_17
            Annotation Date                   :: 03/17/2024 01:57:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,508
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,354
            CDSs (with protein)               :: 4,354
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 36 of 69
            Pseudo Genes (incomplete)         :: 48 of 69
            Pseudo Genes (internal stop)      :: 11 of 69
            Pseudo Genes (multiple problems)  :: 21 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:42:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1031281
            Orig. end                         :: 1041626
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RZIR01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10345
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10345
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10345
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5345](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5345
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            297..1226
                     /gene="pstC"
                     /locus_tag="ELY93_RS05370"
     CDS             297..1226
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstC"
                     /gene_functions="transport (smcogs) SMCOG1214:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612605.1"
                     /locus_tag="ELY93_RS05370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="WP_003183532.1"
                     /transl_table=11
                     /translation="MVQKIEKLSAKERLISSKHNRKMDEIRGRILVASCAFIMMAAAIS
                     ITIFLGNKGLQSFLANGVSPIEFLTSLDWNPTNENPEYGALPFILGSFAVTLLASLIAA
                     PLGIGGAIFMTEIAPSWGRKVLQPVVELLVGIPSVVYGFIGLTVLVPFISQFKSSGSGH
                     SLLAGTIVLAVMILPTVTSISTDALMSLPKNLREGSYALGATRWQTIRKVLIPAALPSL
                     LTAVVLGMARAFGEALAVQMVIGNSRNLFESLLDPAGTLTTIITLNMGHTTYGSVENNT
                     LWSMGLVLLIMSFLFILLIRYLSSRRKL"
     gene            1226..2113
                     /gene="pstA"
                     /locus_tag="ELY93_RS05375"
     CDS             1226..2113
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstA"
                     /gene_functions="transport (smcogs) SMCOG1214:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183530.1"
                     /locus_tag="ELY93_RS05375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="WP_023855211.1"
                     /transl_table=11
                     /translation="MNSKVTDRLATGVFGLIAAVIAAILVGLFTYIIINGVSHISLSFL
                     TSRSSALSSGGGIRDQLFNSFYILALTMLITVPLGVGGGIYMAEYAPANKLTDFIRVCI
                     EVLSSLPSIVMGMFGMLMFVNLTGWGYSIIGGALALTIFNLPVMVRVTEDAIRSVPKEQ
                     KEASLALGVSKWHTIKTVLIPGAVPSIITGAILASGRVFGEAAALLFTAGLTTPRLNFA
                     DWNPFSETSPLNIFRPAETLAVHIWNVNTQGIIPDAEAIANGASAVLVLSVLIFNLAAR
                     WLGSFIHKKLTANK"
     gene            2133..2960
                     /gene="pstB"
                     /locus_tag="ELY93_RS05380"
     CDS             2133..2960
                     /EC_number="7.3.2.1"
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstB"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016885932.1"
                     /locus_tag="ELY93_RS05380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="WP_023855210.1"
                     /transl_table=11
                     /translation="MVQVQMPVLETVRDTIGIAPFPEAKEHILEVKNLNIYYGEKRAVN
                     DISMHVDKHAVTALIGPSGCGKSTFLRSINRMNDLIPSARTSGEIRYEGLNILDSNINV
                     VNLRREIGMVFQKPNPFPKSIYSNITHALKYAGERRKSVLNEIVEESLRKAALWDEVKD
                     RLHESAFSLSGGQQQRLCIARTLAMKPEVLLLDEPASALDPISNAKIEELIVGLKEEYS
                     IIIVTHNMQQASRISDQTAFFLNGDLIEYDRTEKIFMNPSEKKTEDYINGRFG"
     gene            2973..3752
                     /gene="pstB"
                     /locus_tag="ELY93_RS05385"
     CDS             2973..3752
                     /EC_number="7.3.2.1"
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstB"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390374.1"
                     /locus_tag="ELY93_RS05385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="WP_023855209.1"
                     /transl_table=11
                     /translation="MSAVTAVKPKDVYQINDLNLWYGEHHALKNINLTIPEYEITAIIG
                     PSGCGKSTFIKTLNLMINMVPNVKMTGEISYNGTNVLNSKVDLVELRKKVGMVFQKGNP
                     FPQSIFDNVAYGPRIHGLKNKKELNERVEKALVDVALWDEVKDRLHSQALGLSGGQQQR
                     LCIARALATNPDVLLMDEPTSALDPVSTLKIEELMLKLKEKYTIAIVTHNMQQASRISD
                     QTAFFLMGELIECNDTVKMFSSPDDYRTKDYITGRFG"
     gene            complement(<3885..4043)
                     /locus_tag="ELY93_RS05390"
                     /pseudo=""
     CDS             complement(<3885..4043)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611387.1"
                     /locus_tag="ELY93_RS05390"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGTRVNYPVEVKRKALEMTLVGVPKKEIMQKSNIKKKARVQAWGR
                     WYKAADKH"
     gene            4323..4976
                     /locus_tag="ELY93_RS05395"
     CDS             4323..4976
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /locus_tag="ELY93_RS05395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YIP1 family protein"
                     /protein_id="WP_023855206.1"
                     /transl_table=11
                     /translation="MDNKQNFITLVHHFIFSPKDFFSIKYSSDKNYRHSWFVFLAVLFL
                     FLGFYLAIFPTLSTLINNSFQDNGIDYTTDMNTLIMIITLVLGIIQFTSMVLVSFFIIH
                     IIGILIGGTATAKEFFPSFIYSIIPIGIKFTILIIFSFFLGSDFAFSVIYPNSQQPLDI
                     ILNIIIDPFFWWSTILGCIALKVIHQISYKKTIIIISITMSLGIISLFSTSLFL"
     gene            5001..5345
                     /locus_tag="ELY93_RS05400"
     CDS             5001..5345
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03651.1"
                     /locus_tag="ELY93_RS05400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_020452200.1"
                     /sec_met_domain="TIGR03651 (E-value: 1.5e-09, bitscore:
                     36.0, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNSNLFKIASITGVVSLFAIATFLMGSSEGIAGLASIDFSSGHQ
                     FALDLATNLGISKKTAYVAIGVIMTTGDVLTILSLLAVVLGGTGLITAAMVATAKKLAK
                     KYGKKYAAEW"
     gene            5418..7112
                     /locus_tag="ELY93_RS05405"
     CDS             5418..7112
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ELY93_RS05405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_020452199.1"
                     /transl_table=11
                     /translation="MLNKRVLIANFKLTYRRTLNRLIYFFPSSENLLKSNKILFVFAPF
                     FLVYIFILYKASQTLLTGVPEHILTLGTNFIILFFVLNILKSSTDIIHRYVYPEDFLLL
                     HTLKTDPKKILLSRFFTTIFLNILFKLTVVLFPILFFINFSTHDRIYLLLSFPSLLIFI
                     VGLTSLYSSLTYIVYSKFLHNILNSRLKSLLSLIGIACISAISTTFLGWIGLNYFYQND
                     LLEAIGNFIYLFKLDTILTTNWLPTNWFLLSTSDIHLFFLCSIGYIALGCLFAFITFHL
                     ILKVLIIKEAVPNNLLPKLKPIKLPFLSFLQKTLPRQNIAILKKDLTALLRANFTIKKR
                     LVLSINMIAVELGVLIGLSLNQKWFDFSDIFVIYPILFAVTYNTSLLGEGLLGVTSADA
                     ERENLYVYKSSGASIFKLIAGKSLLHLSVILILMNTLFLFILLLFNLDIMTQLILFIMI
                     NSISLIIGAGQIIGTFLYPRLDWENFEDIGSSVKASFFEHTILGVLLAFYLQLYGITGV
                     LVTFNIINRGVFFLIIVTGTILFVIGIGVFYYFWIKRIGTKNWEMKT"
     gene            7109..7816
                     /locus_tag="ELY93_RS05410"
     CDS             7109..7816
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="ELY93_RS05410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_020452198.1"
                     /transl_table=11
                     /translation="MNIENVSFKYKDKLILESINLKIEKGKVYGLLGPNGAGKSTLLKC
                     ILGIVKPYEGKITVKGETLESERSSYLKYIGYVPDAPFIYHYLTPREYLEFVADLQEVP
                     YEIKNDRIDLLLSQFKLKDNENDMISTFSFGMKHKLSVAGAIVHEPEILILDEPLGAFD
                     PPTTKMMKSFLKRYAEQGNTVIMSTHLVNIAQQLCDKVGILINGTLVKEYNHLTDIKDF
                     EDLIIRDMDTILK"
     gene            7813..8367
                     /locus_tag="ELY93_RS05415"
     CDS             7813..8367
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /locus_tag="ELY93_RS05415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage II sporulation protein M"
                     /protein_id="WP_020452197.1"
                     /transl_table=11
                     /translation="MKELIKRNKRILYIASSFYIVGLVLGVIFNNILPSLSQDNVQNIT
                     VSGYFTHNFAANLFLIFSMFTFGILTSILLLLNGFLVGLSSLHSITDHGNDVLFILTAL
                     VPHGIFEIPAMILSGALGFKLLDYVYMKIRGIYFSKKQFLQDFLYFVIILFLLTLLAAV
                     TEALITPYLLNKLTLGGSYVF"
     gene            8357..8653
                     /locus_tag="ELY93_RS05420"
     CDS             8357..8653
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179490.1"
                     /locus_tag="ELY93_RS05420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_020452196.1"
                     /transl_table=11
                     /translation="MFFNKKQIDKLLDDHTEEEQKETKSKVNTSFIIVFYFSILLSICT
                     VFFDYRYFLIALFGPTILVTFISPPFQTKKGIYRIFSAIYLILLITVTFFWLN"
     misc_feature    complement(8791..8802)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.02"
                     /tool="antismash"
     gene            <9167..9418
                     /locus_tag="ELY93_RS05430"
                     /pseudo=""
     CDS             <9167..9418
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611387.1"
                     /locus_tag="ELY93_RS05430"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="CLLCDQG"
     gene            complement(9475..9954)
                     /locus_tag="ELY93_RS05435"
     CDS             complement(9475..9954)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183520.1"
                     /locus_tag="ELY93_RS05435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="endolytic transglycosylase MltG"
                     /protein_id="WP_023855204.1"
                     /transl_table=11
                     /translation="MTRQSVQAFAGGMILATAVLAGAFYLTDNGKAEASKDAQNISETD
                     VKTYLKDNQQVSLKREEYQQLLHYKETALKQDASSQNKKEKEAEKKDSKYKLEIKSGMS
                     TAEIADLLAKGKVISSADDFKDYVKKTGNESKIRAGKYELKRGDSLKNIVKTLSK"
     gene            complement(9951..10301)
                     /locus_tag="ELY93_RS05440"
     CDS             complement(9951..10301)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390372.1"
                     /locus_tag="ELY93_RS05440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_011198121.1"
                     /transl_table=11
                     /translation="MEIAIIALLVVSIALIAFSYFQREPIKEVEQELETLQLSAMQEIY
                     KLKKKMTVLEEELLDSNVVVRRPNAGISQHIAKQILSKYQNGMSVDAIAKAEHVSVEDV
                     KAIIKDNERVLV"
ORIGIN
        1 gcgttaagcc gacagccgaa aatgtacaaa aaggcaaatt cccgatctgg gcataccagc
       61 attcttatac gaaaggcgag cctgaaggct tggcgaaaga attcctcgac tatttgatga
      121 acgatgacat tcaaaaatcc atcgtgacgg agcagggcta cattccagta acagacatgg
      181 aagtcaagcg cgatgcgaat ggaaagcaaa ctgataaata attgacaata gaaggcggga
      241 aagtgtgctc ctgacacttt ccctcttatg acttgttctt ataggagtgt aaaaaaatgg
      301 ttcaaaaaat agagaaactg tccgcaaaag agcggctgat cagctccaag cataatcgca
      361 aaatggatga aatccgcggg aggattttgg ttgcgtcgtg cgcgtttatc atgatggcgg
      421 cggcgatttc gatcacaatt tttctcggaa ataaggggtt gcaatctttt ctggccaatg
      481 gtgtaagtcc aattgagttc ttaaccagtc tagattggaa tccaacgaac gaaaaccctg
      541 aatacggagc ccttccgttt atactcggat cttttgcagt gacccttctt gcatctttga
      601 ttgctgcacc gcttggaata ggaggtgcga ttttcatgac ggaaatcgcg ccatcatggg
      661 gcagaaaagt actgcagccg gttgtcgaat tgcttgttgg tattccttcc gtcgtttatg
      721 gattcatagg ccttacagtg ttagtgccgt tcatttcaca gtttaagtca agcggatcgg
      781 gccacagcct tctggcggga acgatcgtcc tggctgtcat gattttgccg acggtcactt
      841 ctatttcaac ggatgcgctc atgtcgctgc cgaaaaatct gcgcgaagga tcatacgcat
      901 tgggcgcaac ccgctggcag acgatccgaa aagtgctgat accggcagcg ctgccttcgc
      961 ttctgacagc cgtcgtctta ggaatggcga gggcgttcgg cgaagcgctg gccgtgcaga
     1021 tggtcatcgg aaattcccga aacctgtttg agagcctttt agaccctgcg gggacattaa
     1081 caacgattat aacgttgaat atgggccata caacatacgg aagcgtggaa aacaatacgc
     1141 tctggtcaat gggccttgtc cttctgatca tgtcgttctt gttcatcctg ctcatccgct
     1201 atctgtcatc caggaggaaa ctgtaatgaa cagcaaagta acagatcgtt tagcaaccgg
     1261 tgtttttgga ttgattgctg cggtaatcgc cgccattctt gtcggtttgt tcacttatat
     1321 catcatcaat ggtgtttcac atatcagtct cagcttttta acttcgcgtt ccagtgcgct
     1381 ttcaagcggc ggcggaatcc gcgatcagct gttcaactcg ttttatattc tcgcactgac
     1441 gatgctgatc acggttcccc ttggagtcgg cggaggaatt tatatggctg aatatgcgcc
     1501 cgccaacaaa ttgaccgatt ttatccgtgt ctgtattgaa gttctgtcat cccttccttc
     1561 gatcgtcatg gggatgttcg gcatgctgat gtttgttaat ttgaccggat gggggtacag
     1621 cattatcggc ggagcgcttg ctttgacgat tttcaacctt cccgtcatgg tcagggtgac
     1681 agaggatgcg atccgctctg tacctaagga acaaaaagaa gcttcccttg ctttaggtgt
     1741 gtccaagtgg catacgatta aaacggtctt gatccctggt gcggtgccgt ccatcatcac
     1801 aggggcgatt cttgcgtcgg gccgcgtatt tggcgaagct gccgctttgc tgtttacagc
     1861 cggactaacc acgccgagac tgaatttcgc cgattggaat ccgttttctg agacctcgcc
     1921 gctcaatatt ttcagaccgg ctgaaacgct ggccgttcac atttggaatg taaacacaca
     1981 ggggatcatc cctgatgctg aagcgatcgc aaacggggca tccgccgttc ttgtactgtc
     2041 cgtcttgatc tttaatttgg cagcccgatg gctcggctct tttattcata aaaagttaac
     2101 ggcaaacaaa taagaaggaa ggatgaaatc atatggttca ggttcagatg ccggttttgg
     2161 aaaccgtgcg cgacactatc ggaatcgctc cgtttcctga agcgaaagaa catattttag
     2221 aagtgaaaaa tttaaatatt tactatggag aaaagcgggc cgtcaacgac atttccatgc
     2281 atgttgacaa acatgccgtt acggctttga tcggcccgtc aggctgcgga aaatcaactt
     2341 ttttaagaag catcaaccgg atgaacgatc tgattccatc agccagaaca tccggagaaa
     2401 tacgatatga aggacttaac atccttgatt ccaatatcaa tgtcgtcaat ctgagaaggg
     2461 aaatcggaat ggtatttcag aagccgaatc cctttccaaa atcgatttac agcaacatta
     2521 cccatgcttt aaagtatgcg ggtgaacggc gtaaatctgt gcttaatgag atcgtggaag
     2581 aaagcttgag gaaagccgcg ctgtgggatg aagtgaaaga caggctgcat gagtcggctt
     2641 tttcgctgtc aggcgggcag cagcagcgcc tctgcatcgc ccggacgctc gcgatgaagc
     2701 ctgaagtcct gcttttggat gagcctgctt cagcgctcga tccgatttca aatgcgaaaa
     2761 tagaggaatt aatcgtcgga ttaaaagagg aatactcgat catcattgtc acgcataaca
     2821 tgcagcaagc ttcaagaatt tccgatcaaa cggcattttt ccttaacgga gatttgatcg
     2881 aatacgatcg tacggaaaag atctttatga atccttcaga aaaaaagaca gaggattaca
     2941 tcaacggcag gtttggataa ggaggggccg gtatgagcgc tgtaacagcc gtaaagccaa
     3001 aagatgtgta ccagatcaat gatttgaatt tatggtacgg cgaacatcac gcattaaaga
     3061 acattaactt gacaataccc gagtacgaaa ttaccgccat catcggtccg tcaggctgcg
     3121 gaaagtcgac cttcattaaa acgctgaatc tcatgatcaa tatggttccg aatgtcaaaa
     3181 tgaccggaga gatcagctac aacggaacga acgtactcaa cagcaaagtc gatcttgttg
     3241 agctgcggaa gaaagtcggt atggtgtttc aaaaaggaaa tccgtttccc cagtcgattt
     3301 ttgataatgt cgcatacgga ccaaggatcc atggactgaa aaacaaaaag gaattgaatg
     3361 agagggtgga aaaagcgctt gtggatgtgg cgctgtggga tgaagtgaaa gaccgccttc
     3421 attcccaggc gctcggactt tccggggggc agcagcagcg cctctgcatc gcaagggcgc
     3481 tggccaccaa tccggacgtt ctcttgatgg atgaaccgac atcagctctt gatccagttt
     3541 cgactttgaa aattgaagag ctgatgctca agctgaaaga gaaatacacg attgcaatcg
     3601 tcacgcacaa tatgcagcag gcatcgagaa tttccgatca aacggcattt ttcctcatgg
     3661 gcgagctgat tgagtgcaac gatacggtca aaatgttttc aagtcctgac gactatcgga
     3721 caaaagatta tattacaggc cggttcggat gagaaagagg ctgccgcttt aaagcggcag
     3781 ccttttttgc accggagcgg aggcaataaa aaagcaggct ggtacgaagg ggaccggtcc
     3841 cataagatga gacaaataaa aaacaccttc aactgttcga atcagtgttt atccgcagcc
     3901 ttataccacc tgccccatgc ctgaacccgc gcctttttct tgatattcga tttctgcatg
     3961 atctctttct taggtacgcc taccaatgtc atttctaaag ccttccgttt gacttcaacc
     4021 ggataattca ctcttgtccc catagaaaaa acacctccac gtcttctgtt ggaaaacaat
     4081 atacttatcg aaaggatccc gagtattctt ctgaatgcag gcagaaagtc gttagctgaa
     4141 acaacaaaat gaagttttaa aaaagggggt agtcccagag gcattctttt cgattttcac
     4201 gaaacaattc aaccttaaat ttaccttaat aaataaaatc ccatattgac caaacattgt
     4261 aaaaggtgat atgtttttaa tagaattaac aaaaaatact tttgtttata taaggagcat
     4321 ttatggataa taaacaaaac tttattactc tcgtacatca tttcatattt tctccaaagg
     4381 actttttttc tatcaagtat tctagcgata agaattatcg tcattcttgg tttgtcttcc
     4441 tagctgtatt gtttctgttc ttaggctttt atttagctat cttcccaaca ctctctacct
     4501 tgattaataa ttcatttcaa gacaatggaa ttgattatac aactgatatg aatacactca
     4561 ttatgatcat tacattagta ttaggtatta ttcaatttac ctcgatggtt cttgtaagct
     4621 tttttattat acatattatt gggatattaa ttggcgggac cgctacagcc aaagaatttt
     4681 tccctagttt tatttattca atcataccta taggcatcaa atttacaata ctcattattt
     4741 tttctttctt tttaggtagt gatttcgcct tttcagttat ttacccaaat tctcaacagc
     4801 cgctagatat tatcttgaat attattatcg accctttctt ttggtggtct actattctag
     4861 ggtgtattgc cttaaaagta atacatcaaa tttcttataa aaaaaccatc ataatcatta
     4921 gcattacaat gtctttaggt attatttctc ttttttcaac aagtttattt ttataaactg
     4981 aaaggaggtg agatttcaag atgaaaaata gcaatctttt taaaattgca agtattacag
     5041 gtgtagtttc tctctttgcc attgctactt tccttatggg ctcttcagaa ggtattgcag
     5101 gtttagcttc tattgacttt agttcaggtc atcagttcgc tttagacctt gcaacaaacc
     5161 taggaattag taaaaaaaca gcctatgtag caatcggtgt tattatgaca acaggcgatg
     5221 tattaacaat tctcagccta ttggctgttg ttttgggcgg aactggtcta attacagctg
     5281 caatggtagc tacagcgaaa aagctagcta aaaaatacgg caagaaatat gctgctgaat
     5341 ggtaagatgc tcaaaaggaa gagattgagg agtataatta tactcctcct tattttcgtt
     5401 ctaggaggaa tatacacatg ctcaacaaaa gggttctcat tgcaaacttt aaactaacct
     5461 atagacgaac tttaaatcgc ttgatttatt ttttcccatc aagcgaaaat ttacttaaat
     5521 caaataaaat tctatttgtc tttgcgccct ttttcttggt gtatatattt attctatata
     5581 aagcctcaca gactctttta actggagttc ccgagcatat tctaactcta ggtactaact
     5641 ttatcattct cttttttgtt ttgaatatat taaaatcatc gactgatatc atacatcgat
     5701 atgtatatcc tgaagatttt cttcttcttc atactttaaa aacggatccc aaaaaaattt
     5761 tattatcaag gttcttcaca acaatttttc taaacatcct gtttaaatta acagtagtgt
     5821 tattccctat actctttttt ataaattttt ctacacatga ccgtatatat cttttgttat
     5881 catttccatc actgctaata tttattgttg gactgacaag tctttattca tcgcttacat
     5941 acatcgttta ttctaaattt ctacataaca ttttaaattc acgattaaaa agcttgttat
     6001 cccttatagg aattgcctgt atatctgcca tttcaactac ttttcttggt tggatcggac
     6061 ttaattattt ttatcaaaat gatctcctcg aagctattgg caatttcata tatctattca
     6121 aattagatac gatattaacc accaattggc tgcctacaaa ttggttttta ctttcaacaa
     6181 gcgatataca tttatttttt ttatgttcta taggttatat tgctttaggt tgccttttcg
     6241 catttattac ctttcattta attttgaaag tattgattat aaaagaagca gtacctaata
     6301 atcttttgcc aaaattaaaa ccaatcaagt tgcctttttt aagtttctta caaaagacct
     6361 taccacggca aaatattgct attctaaaga aagaccttac tgcattatta agagctaatt
     6421 ttacaataaa aaaacgactg gtactttcca ttaatatgat tgcagtagaa ctaggtgtat
     6481 tgataggtct tagtttaaat caaaagtggt ttgatttctc agacattttt gttatctacc
     6541 caattttatt tgctgtgact tataatacat ctttattagg agagggatta cttggcgtaa
     6601 cttcagcaga tgcagagcgt gagaaccttt acgtttataa gagctcgggt gcttctattt
     6661 ttaaattgat cgcaggaaaa tctttattgc acttatctgt gattcttatt ttaatgaata
     6721 cgttattttt attcatctta ttgttattca acttagatat tatgacgcag cttatattgt
     6781 tcatcatgat taactccatc agtcttatca taggggctgg acaaattatt ggaacatttc
     6841 tctatcctcg tttagattgg gagaactttg aagacattgg ttcatctgtt aaagcatcat
     6901 tttttgaaca tactattctc ggagtgttac tggcatttta ccttcagttg tatggtatca
     6961 caggcgttct cgttactttt aatattatta ataggggagt attcttttta atcattgtga
     7021 caggtaccat tttatttgtt ataggtattg gagtctttta ttatttttgg attaaaagaa
     7081 taggaacgaa aaattgggag atgaaaacat gaacattgaa aatgtgtctt tcaaatataa
     7141 agataaactg atccttgaat ctattaattt aaagatagag aaaggcaaag tttacggctt
     7201 acttggacca aatggagctg gtaaatcaac tcttttaaaa tgtatccttg gtatagtgaa
     7261 accttacgaa ggtaaaatca ctgtcaaagg agaaaccttg gaatctgaaa gatcctctta
     7321 ccttaaatat ataggatacg ttccagacgc accttttatc tatcattatt taacacctag
     7381 agagtacctt gagtttgttg ctgatttaca agaagtccct tatgagatta aaaatgatag
     7441 aatagatctt ttgttaagtc aatttaaatt gaaagataat gaaaacgaca tgatatctac
     7501 attttctttt ggtatgaaac ataagttatc tgttgcagga gccatagttc atgaaccaga
     7561 aatattaata ttagatgaac cattaggggc atttgatccc cccactacaa agatgatgaa
     7621 aagttttctc aaacgttatg ctgaacaggg gaatactgtc atcatgtcta cacaccttgt
     7681 aaacatagcg caacaactat gcgataaggt tggtatactg attaatggta ctttagttaa
     7741 agaatataat cacctgacag acataaagga ttttgaagat ttgattataa gggatatgga
     7801 tacgatacta aaatgaagga attaataaaa agaaataagc gtatactcta tatagcaagc
     7861 tccttttaca tagtgggttt agttcttggt gtgattttta acaatattct acctagcctt
     7921 agtcaagata atgttcaaaa tatcacagta tcaggttact ttacacataa ttttgcagca
     7981 aatttatttc tcattttttc aatgtttaca tttggaattt tgacatctat cttattacta
     8041 ttgaatggct tcttagtcgg tctatcttct ttacatagta tcactgacca tggaaacgat
     8101 gtcttattta tattaactgc tttggttcct cacggtattt ttgagattcc ggcaatgatt
     8161 ttgtcgggag cacttggttt caaattgtta gattacgtgt atatgaagat aaggggtatt
     8221 tatttttcaa aaaaacagtt tttacaagat tttttgtact ttgttattat acttttttta
     8281 ttgacattac ttgctgctgt tacagaagca ttaataacgc cttatttact taacaaatta
     8341 acattaggag gctcttatgt tttttaataa aaagcagata gataaattac tggatgatca
     8401 tacagaagaa gaacaaaaag aaacgaaaag taaagttaat acttcattta taatagtatt
     8461 ttatttttca atactactat caatctgtac tgtctttttt gattataggt actttttgat
     8521 tgctttgttt ggtccgacca ttttagtaac ttttatatcc ccacctttcc aaaccaaaaa
     8581 aggtatttat agaatttttt cggccatata tttaatactt ttaattacag ttactttctt
     8641 ttggttaaat tgattgctta attctcaatc aaaaaacgtc cttaatggat gtttttttat
     8701 atcgtttaaa ttaataattt gaattgcttt ttgacaagtt caacttcgta ctacgaatgg
     8761 gttctccatc tttttttatt gtataggtat gtaaacggtt tctgtagggg gagggcttat
     8821 gttaacgttt actggtttgg ggaaagcaag tccgaccatg gtgccttttg taggggtgct
     8881 tatttctgga tttggcatca atgatattta aagaagggat tatcaaagta ctgccagtgg
     8941 cgaccatgtt tatcttcgtc actttaattt ttgcattcta caagacacag gattctttga
     9001 acctctaatc aaagcattaa acagctgaca aaggggaatg ttgtcactgt ttccatgggt
     9061 acagctgcaa gtgggatgat ctcacactta gatggtgcag gagccacaac ctttttatga
     9121 actgtacccg cactgcttca gcttgtaaaa attacatatg ggtccttgct tactgtgcga
     9181 tcaaggttaa gtgtatacat cttatgagta tcagaaagct attaatataa tgcctccatc
     9241 ggatcgtttc attcctcact aaagtctgaa acgtttgatc ttaacagcat tgatcgaact
     9301 acgactgcca tcgtagaaca cactgttaaa gaaatacatt cattatttta acaatattcg
     9361 tattaaaaca aaactaaaca accaatcacc ggtaaactat cgggaattgg ttgtttaaag
     9421 gtgtttttaa atcctgtctc aaaacggggg tcagtcccgt tccagcttgc ttttttattt
     9481 ggacagtgtt ttgacgatgt ttttcaagct gtcaccgcgc ttcagttcgt acttacctgc
     9541 gcggatttta gattcgtttc cggtcttttt cacataatct ttgaagtcat cggcagatga
     9601 aatgactttt ccttttgcaa ggaggtctgc tatctcagcc gtgctcatgc cgcttttgat
     9661 ttcaagctta tacttggaat cttttttttc tgcttctttc tcttttttgt tttgagacga
     9721 agcatcctgc ttcagagcgg tctctttata atggagcagc tgctggtatt cttcgcgttt
     9781 cagcgaaacc tgctggttgt cctttaaata ggttttgaca tcggtttcgc tgatgttttg
     9841 agcgtcttta gaagcttcag cttttccgtt atccgtcaaa taaaatgcgc ctgcaagaac
     9901 agcggtagcc aaaatcattc caccggcaaa tgcttgtaca ctttgtctgg tcatacaagc
     9961 accctctcat tgtctttaat gatcgctttc acatcttcga cagatacgtg ctcagcttta
    10021 gcgatggcgt ctacagacat gccgttttgg tattttgaaa gaatctgctt cgcaatatgc
    10081 tggctgatgc cggcatttgg tctgcggacg accacatttg aatcgaggag ctcttcctca
    10141 agcacggtca tcttcttttt caatttatag atttcctgca tggcggacag ctgaagggtt
    10201 tccaactcct gttcaacttc cttgatcggt tctctttgaa aatatgagaa tgcaatcagc
    10261 gcaatgctga caacgagcag cgcaatgata gcaatttcca cgttcatcac cttcaattct
    10321 aattttctcc ttctttttta tacca
//
