LOCUS       NZ_RZIR01000001        11362 bp    DNA     linear   CON 17-MAR-2024
DEFINITION  Bacillus sp. AIIW2 Scaffold_1, whole genome shotgun sequence.
ACCESSION   NZ_RZIR01000001
VERSION     NZ_RZIR01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. AIIW2
  ORGANISM  Bacillus sp. AIIW2
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 4396332)
  AUTHORS   Kumari,A., Chaudhary,D.R. and Jha,B.
  TITLE     Plastic degrading bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4396332)
  AUTHORS   Kumari,A., Chaudhary,D.R. and Jha,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2018) Biotechnology and Phycology, CSIR Central
            Salt and Marine Chemical Research Institute, G B Marg, Waghawadi
            Road, Bhavnagar, Gujarat 364002, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 08-JUN-2018
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 99.95x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009932115.1-RS_2024_03_17
            Annotation Date                   :: 03/17/2024 01:57:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,508
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,354
            CDSs (with protein)               :: 4,354
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 36 of 69
            Pseudo Genes (incomplete)         :: 48 of 69
            Pseudo Genes (internal stop)      :: 11 of 69
            Pseudo Genes (multiple problems)  :: 21 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:42:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 884239
            Orig. end                         :: 895601
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RZIR01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11362
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11362
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11362
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6362](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6362
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(261..1277)
                     /locus_tag="ELY93_RS04580"
     CDS             complement(261..1277)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /locus_tag="ELY93_RS04580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="iron ABC transporter permease"
                     /protein_id="WP_051303761.1"
                     /transl_table=11
                     /translation="MNSRNRFVMLLFLNVFFLIVFFSLSMILENNASPLSVISDMINGT
                     APAYRYEVIQDLKIPRGLTAILAGGMLGLAGAMIQSLSRNPLAEPGLIGVTAGGVCAAV
                     LWITFGPAHLQLSFFLPFFACFGGILAAFFVYVLSSYTKKMSSVFLLNGILISAVLQAV
                     TSFILLRHQEALGSILLWTIGSLNGRVWTHLNLLWPIAVPALIAGLLTAGIANAWRLGT
                     ESAVLLGLSLKKTNALLFFISALLTAGAVSIVGAIGFIGLIGPHIAKRIVGEDARKSFP
                     ISMLLSSLLLLTADLIAQYIFSFLPFSFISQNIQLPAGAVTVIMGVPFFLYLLRKQT"
     gene            complement(1274..2278)
                     /locus_tag="ELY93_RS04585"
     CDS             complement(1274..2278)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /locus_tag="ELY93_RS04585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="iron ABC transporter permease"
                     /protein_id="WP_023855316.1"
                     /transl_table=11
                     /translation="MGVKHKRLLILYLVLALSCIYLFYLSLSAGSPSASFSSVFRILTK
                     GGESDPLLYVSIMEIRMPRAMIAFFIGMMFALSGTILQDTFHNGLAGPELLGVSAGASL
                     TMAVLTVLHLPVAFYLHPFISLAGGLAGGIVVILCTMGKANKQNMILIGAAVSAFFQSL
                     IIITISIGSQNDIGLLFFYLIGSLANRNWDHVIEIAPWFLVCVPAALAMAKPLNVLRLG
                     DEAAQGRGVSVVKMRTFLLFLSVSLSAAGVAVCGPVGYIAFIAPHLARRIMGTSNAFVI
                     LPLSGLIGSCTLMGTDLSARMLFYPQELPVGLFTVMFGGLLLLFFLNKRRKST"
     gene            complement(2284..3258)
                     /locus_tag="ELY93_RS04590"
     CDS             complement(2284..3258)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1033: iron
                     compound ABC transporter, periplasmic"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /locus_tag="ELY93_RS04590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_023855315.1"
                     /transl_table=11
                     /translation="MKKAIFLIAMFIVILSSCSSQPENSNTSHNKGSQKKEMSYTDDTG
                     HKISFSKTPKKIICLTEICSDTLHELGLKPEAVLEDGLVSQDEFYGKDHQIPLIGGTFY
                     EPNLQNIYQYEPDLVIGLGGVHDNLRDGLGDKAPLFLVNPNHYQDSLSFVETFGSMLGE
                     EKKAKQLTNDFKKKLEQVKKMSPKNKKALVMYGSDINFGIDTQSSIVGSILNEITDYPW
                     KVKGKQEGHNAGGTKYSLEEILKENPDVIFVETFNFGNNKGPSLSEQLANNRIWKRLKA
                     VKNDNVHEVRTSIWANGRGVGSLTIILREAMEQLYPTIDVEKE"
     misc_feature    3439..3450
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.25"
                     /tool="antismash"
     gene            3443..5035
                     /locus_tag="ELY93_RS04595"
     CDS             3443..5035
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /locus_tag="ELY93_RS04595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_023855314.1"
                     /transl_table=11
                     /translation="MISFRWEVAAEDGAMLCADAYVPAETGAFPVILTRTPYGKSAFSE
                     TAKAWCELGVAFVAQDVRGRYQSGGRWIPYSAEAKDGAATLKALKRASWCNGHIALMGE
                     SYGAFTAFLAAQSEIGRQTPSALITLVPAMGLKKTAFTSDRVFYLGDRLWWDASFGSSR
                     ENIEHLFSAVLNKHSSAVFTLPVIDIPTVFPMQLANWRRILENEHNEESLNLESVHQPC
                     LHIGGWYDPFVSHTIHNFKTLRKKEKRQALVIGPWSHEVNRIPAFEILHDQKHAVIPLG
                     SLEHAWLESIWSDKLYPFPEILLYVTGADQWIAPEEWPPAGTMDFTLYLRECRLSTDMP
                     AGGENTSSSYTYDPLQPVPSQEYPSKRNEIEKRADVLVFTSAPFVRDIILSGAPAVKLW
                     VSSTAPSTDFIVVITEVYPDGTSVYLTHGHIRTGDEKGGIRPQDVTISMEPRCHKIKNG
                     RRIRLSISSSSFPKYARHLNRNGDQHLQSSPQIAEQTIWHSEHMLSHLKIPIENSLLER
                     CSYEFTPGWPTAH"
     gene            5001..6362
                     /locus_tag="ELY93_RS04600"
     CDS             5001..6362
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /locus_tag="ELY93_RS04600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_023855313.1"
                     /sec_met_domain="TIGR03604 (E-value: 3.1e-77, bitscore:
                     257.9, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 2.4e-63, bitscore: 212.9,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLPLDGQLLIDKRTGIINGLQKVDIEPVLPRKAVSWRAQVTNGR
                     ILANIPSDRIAGGTHFSNDQARNAAIGEAIERYSGNYKGGPFIRGSYQELKRKGYKLLD
                     PDRIPLYSTWQYAEKGFPFIPFTKDLKVNWVQGFSLTCKEEVLLPASLVYINYYEGIFA
                     NEPPVHFVNYAGIASGPTLEFALKSGLLELIERDATAIWWGCRGQIDAIDINDHPDLLN
                     SLEAREESEIRYTFLPLTTDINIPVIGAMLYDPVRQIACMGFSSRPDPKKALLKAAAET
                     VQSYHLSLDLLDPDSPLWDAVHTGILHQAALHSYRKDRKYVQSFKSDYHDMIDLLHNSQ
                     YYLDPDSHQDIRHIFDAEEKGMLPEKQSLTSSDIAEALAKHQIECFYADVTTEDVRTAG
                     FTVTRVIAPGLVPNAPAAFPYLAPKRLYEVPYRIGWSEHVLDEHHINLKPIPHS"
     gene            6410..7003
                     /locus_tag="ELY93_RS04605"
     CDS             6410..7003
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /locus_tag="ELY93_RS04605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_023855312.1"
                     /transl_table=11
                     /translation="MPFLCETAPVRTYARNQIVPLENSRIYIVQSGLVFIWETHLDGRQ
                     VLVNILDGNSAIQIHNFVHASYEMQIKKTSFLQITFLEAIDNQMNILHALRKQTFMSEE
                     LAAIHREKYVEHRLYFFLQFLKRHFGKEIKKEHYLIAVPITHEELASAVNSTRPTITKL
                     LTKMIDKNQVVYQQNGKNRWIILKPLFFSAAEPF"
     gene            complement(7060..7203)
                     /locus_tag="ELY93_RS04610"
     CDS             complement(7060..7203)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ELY93_RS04610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_161513139.1"
                     /transl_table=11
                     /translation="MSKQVSEASKTLLEVLDEGRTFAVDPHHHALVHNPFVWEEEKDNE
                     EN"
     gene            7375..8151
                     /locus_tag="ELY93_RS04615"
     CDS             7375..8151
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ELY93_RS04615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_023855310.1"
                     /transl_table=11
                     /translation="MTHYIFPHHMIPFLGSDQQAYLMNEEGEIMKVGFLNERSNMYWGR
                     LKPGASIQLPDEETELIHVMNEISQREEFISPSIHMVTDDSFSKKLEAKFEQSGFFISS
                     TNQKGIHIVFSGFFNCDSFQRFSDDAQENQLHWLPLYFDGRIARVGPLLGPYLQFEDLR
                     KRLLAAAYFPDVTDSLWKAAQTAPFSEHHLPLVHLEWLLTQISWIVHDYSSANQLRLHM
                     HQISLDPANMETKRHPILKVPSPIPSHERKDECHVR"
     gene            8141..8716
                     /locus_tag="ELY93_RS04620"
     CDS             8141..8716
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /locus_tag="ELY93_RS04620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_049936996.1"
                     /transl_table=11
                     /translation="MSANVLGLLGSLRYPSKTEEALRICLKAAERRGCTTSIFDGRITP
                     LEFCDGRDEEDTYVEGVALLREKVREADAIVIASPEYSGSYSAFAKNMIDFLGPDYLQG
                     KWAGVIGVAAGNSAANTVAHLSAILARNGCWLVPTPVELPASHTIFSQHASSPTAKHLV
                     SLLNALGENIAFSVNQINRRQYENHTSQ"
     gene            8694..9236
                     /locus_tag="ELY93_RS04625"
     CDS             8694..9236
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /locus_tag="ELY93_RS04625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family protein"
                     /protein_id="WP_023855308.1"
                     /transl_table=11
                     /translation="MKTIQVSETVMLREFKTEDAESLFELIDRSRNHLIEWLPWVDGVK
                     SVKDSQGFITFAQEEAVSGSSLHLGIFEKGRLAGKVCLYQINDRIKTASIGYYIGADFE
                     GRGLVTQAVKQLAAFAFQTRGLNRLEIKMISQNKRSLAVPQRLRFSYEGCLRESGEFQG
                     KPADQLIFSLLKREFQS"
     gene            9479..10102
                     /locus_tag="ELY93_RS04630"
     CDS             9479..10102
                     /EC_number="2.-.-.-"
                     /GO_function="GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /GO_function="GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328063.1"
                     /locus_tag="ELY93_RS04630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="L,D-transpeptidase"
                     /protein_id="WP_023855307.1"
                     /transl_table=11
                     /translation="MKHKRRIGLLIVASVSLVGTMAACSKSKAEMEQVNAEVNQPADTS
                     STETSKNVKTKTEKKEIDWTKPSDGKYPDIKNNDSIWIDVSTKKQEAYIKDGSKTIYTM
                     NISSGLDQTEDDSTPKGTYYVEPERGEWFFSPKYQEGAEYWVSWKNHGEFLFHSVPFNK
                     DKKVIEKEAKKLGKKASHGCIRLAIPDAKWIYENIPEKTKVVIS"
     gene            10487..11194
                     /locus_tag="ELY93_RS04635"
     CDS             10487..11194
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183759.1"
                     /locus_tag="ELY93_RS04635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA alkylation repair protein"
                     /protein_id="WP_023855306.1"
                     /transl_table=11
                     /translation="MDLETVMKELEALGTENMKKRYITNGAHEPLFGVATGKMKPLAKK
                     IKKNQPLAEQLYATGNYDAMYFAGIIADPITMTEADFERWIDAAYFYMLSDYVVAVTLA
                     ETDFAQAVADKWISSGEELRMSAGWSCYCWLLGNRSDDEFNVTKIAGMLDQVEHAIHDS
                     PDRTKSAMNNFIYTVGISYLPLHEKAVETAKAVGPVELRRDKKKSSILNASKNIQMDVE
                     KGRLGFKRKYVRC"
     misc_feature    complement(11209..11223)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.94"
                     /tool="antismash"
ORIGIN
        1 gtctgtgcca gctgctttgc cgaatagctg gtaagcggct ttccgtgaat agaaattcgt
       61 ccagaatcgg gtttcagcaa tttggatatc gtttggagaa gggtggactt ccccgaacca
      121 tttgtgccaa gtatcccggt tatttcattg ttggggatgt tcacagacag attgcgaata
      181 atgttgttat ttgagattga tacagacagg ttttcgatcg taatggcatc catggtatga
      241 ttgatagcac ctcgctttta tcatgtttgt tttcttaata aataaagaaa aaaaggcacg
      301 cccataatga cggttacggc tcctgcggga agctggatgt tttggctgat gaaagaaaaa
      361 ggcaagaatg aaaatatata ctgtgcaatt aaatcggctg ttaacagcaa aagagaagat
      421 agcagcatac tgataggaaa gctttttctc gcatcctcgc caacgatccg tttggcaatg
      481 tgagggccga ttaaaccgat aaagccaatt gcgccaacaa tcgaaaccgc tcctgctgtt
      541 aaaagggcag agatgaaaaa taaaagagcg tttgttttct ttaaagaaag gcccaggagc
      601 acagcagatt ctgttcccag cctccaggca tttgctattc ccgctgtcaa caatcccgca
      661 atgagcgcag gaacagcaat tggccataaa agattcaagt gagtccaaac ccttccattc
      721 aatgatccga ttgtccacaa taaaatgctt ccaagcgctt cttggtgtct tagcaaaata
      781 aatgaagtca cagcttgcaa taccgctgaa attaaaatgc cgtttaacaa aaagacagac
      841 gacatcttct tggtataaga gcttaataca taaacgaaaa aggccgccaa aattccgccg
      901 aagcatgcaa agaatggcag aaaaaatgaa agctgcaaat gagcaggacc aaatgtaatc
      961 cataaaacag cagcgcaaac tccgccagct gtaacaccta tcagccccgg ttcagccaat
     1021 ggatttctcg ataaggattg aatcatagcg cctgcaagcc caagcattcc gcctgccaat
     1081 atcgcagtta atcccctcgg aattttcaga tcttggatca cttcataccg gtaagcagga
     1141 gcagttccat tgatcatgtc agaaatgaca gacagggggg atgcgttgtt ctccaggatc
     1201 atggaaagcg aaaaaaacac gattaaaaag aatacgttca aaaaaagcag catgacaaat
     1261 cggtttctag agttcatgtg ctcttcctcc ttttatttaa aaagaacaag agcaataaac
     1321 cgccaaacat tacggtaaac aatccgaccg gcagttcttg cggataaaac aacattcttg
     1381 cactcaaatc ggttcccatt aacgtgcagc ttccgatcaa ccctgacagc ggtaaaatga
     1441 caaaagcatt gctggttccc attatgcgtc tcgctaagtg aggcgcaata aatgctatgt
     1501 atccaacagg gccgcatacg gcaacgccgg cggctgaaag cgaaacactg agaaataaaa
     1561 gaaaagtacg cattttcacg actgaaacgc ctctgccttg ggccgcttca tctccgagtc
     1621 taagaacatt caacggcttt gccatggcca aagccgccgg tacacaaaca agaaaccagg
     1681 gggcgatttc tatcacgtga tcccaatttc tgttggcaag actgcctatc aaataaaaaa
     1741 atagcaaacc gatatcattt tgactgccga tactgatggt gataatgatt aaactttgga
     1801 aaaaggcact gactgcagcg ccgattaaaa tcatattttg tttattggct tttcccatcg
     1861 tgcataaaat aacaacgatc cctccagcca gtcctccagc caggcttata aacggatgga
     1921 gataaaacgc tacaggcaaa tgcagaacag tcaacactgc cattgtgagt gaagcgcccg
     1981 cactaacacc taatagttca ggaccagcca gaccattatg gaaagtatcc tgcaggatag
     2041 ttccgctcag cgcgaacatc attcctatga aaaaggcgat cattgcccgc ggcatccgaa
     2101 tctccatgat ggaaacgtaa agcagcgggt cactctctcc gccctttgtc aatatgcgaa
     2161 acacactgct gaaagaagcg gaaggagaac ctgctgaaag ggaaagatag aataggtata
     2221 tacatgataa agcaaggacg agatataaaa tcaataatcg tttatgcttc acgcccattt
     2281 attttattcc ttttcaacat caatcgttgg atataattgt tccattgctt cacggagaat
     2341 aattgttaac gaaccgacac cccgaccatt tgcccaaatc gaagtccgga cttcatggac
     2401 gttgtcattt ttcacggctt tcaatcgttt ccaaatgcgg ttattagcaa gctgctctga
     2461 taatgaaggg cctttattat ttccgaaatt gaaggtttcg acaaagatga catcgggatt
     2521 ttcctttaag atttcttcca gcgagtattt ggttccaccg gcattgtgtc cttcttgctt
     2581 tcctttgact ttccacgggt aatcagttat ttcatttaaa atagaaccga caatggaact
     2641 ttgtgtatca atgccgaagt taatatcaga cccgtacata actaaagcct ttttattttt
     2701 cggcgacatc tttttaactt gctccaattt ctttttaaag tcatttgtca gctgttttgc
     2761 tttcttttct tcaccaagca tcgaaccaaa tgtttcaacg aaagaaagcg agtcttgata
     2821 atgattggga ttgactaaga agagaggagc tttatcgccc aggccgtctc ttaaattatc
     2881 atgaacaccg ccaagcccaa tcaccaaatc cggttcatat tgataaatgt tctgaaggtt
     2941 aggttcataa aacgtgccgc cgattaatgg aatttgatgg tcttttccat aaaattcgtc
     3001 ttggctgaca agtccgtctt cgagcacagc ttcaggcttc aaacctaatt catgcaatgt
     3061 atcactgcaa atttctgtta aacaaataat ctttttaggt gttttcgaga aagaaatttt
     3121 atggcccgtg tcatctgtat acgacatttc tttcttttga ctgcctttgt tatggctagt
     3181 attactgttc tccggctggc ttgagcagga agaaaggata acgatgaaca tggcaatcaa
     3241 gaaaatggct ttcttcatat ggatgcccct ttcaaatcgt aattgttact atttataagt
     3301 ttaataacat tttacaacgt tggcaagtat ggaaaaaata aaaattcttg acatataaag
     3361 gaataaaaat tatgatgagt ttcaaatcgt aaccatttcg atttgattgt tggatgaatg
     3421 acaaatggaa aggagggcga aaatgatttc ttttcgatgg gaggtggctg cagaggatgg
     3481 agccatgctt tgcgcagatg cttatgtacc ggctgaaacc ggtgcatttc cagttatact
     3541 cacgagaact ccctatggca aatcagcatt ttccgagaca gctaaagcgt ggtgtgaatt
     3601 aggtgtcgcg tttgtagcgc aagatgtaag agggcgctat cagtcaggcg gaagatggat
     3661 accgtattct gcagaagcaa aggatggtgc agcaacgctt aaagcgttga agagggcttc
     3721 gtggtgcaac ggacatattg cattaatggg tgaatcttat ggagcgttta cagctttttt
     3781 agctgctcaa tcggaaattg gcaggcaaac gccgtcagct ttaattacac tcgtgcctgc
     3841 aatgggactg aaaaaaactg cattcacttc tgatcgcgtt ttttatctcg gcgaccggct
     3901 ttggtgggat gcctcattcg gttcttcacg agaaaatata gagcatctgt tttcagccgt
     3961 attgaacaag cattcttccg ctgttttcac attgccggtg attgacattc caacagtctt
     4021 tcccatgcag cttgccaact ggcgccggat tttagagaat gagcacaatg aagaatcgtt
     4081 aaacttggag tctgtccatc agccttgttt acatattgga ggatggtacg atccatttgt
     4141 atcccacacc attcataatt ttaaaacctt aagaaaaaaa gagaagcgtc aggcgctggt
     4201 gattggcccg tggagccatg aagtgaaccg gattccagcc tttgaaatct tacatgatca
     4261 aaaacatgcc gtgattcctc ttggctcgct tgaacatgca tggcttgaga gtatatggtc
     4321 tgacaagctg tatccatttc ctgaaatcct gctgtatgtg acaggagctg atcaatggat
     4381 agcaccagag gaatggccgc cagccggtac aatggatttc actttatatt tacgcgaatg
     4441 ccggttgtcg actgatatgc cggcaggggg agaaaacaca agttcctctt acacttacga
     4501 tcctttacag cctgttcctt cccaagaata tccttctaaa agaaatgaaa ttgaaaaaag
     4561 ggcagatgta ttagtattta cctcagcccc ctttgttcgt gacatcattt tgtccggtgc
     4621 tccggccgta aaattatggg tcagctctac cgctccttca accgatttta ttgttgtgat
     4681 aactgaagtt tatccggatg gaacgtcagt atatttaaca catggccata taagaacagg
     4741 cgatgagaaa ggaggtatca gaccgcaaga cgtgacgata tcgatggaac cgagatgcca
     4801 caaaataaaa aatggccgcc ggattcgtct ttcgatatcg agcagttcat ttccgaaata
     4861 cgccaggcac cttaatcgaa atggagatca acaccttcag tcttcacccc aaattgctga
     4921 acagacgatt tggcattctg aacacatgct gtcacatctc aaaataccaa tagaaaattc
     4981 attgttagag aggtgttcat atgagtttac ccctggatgg ccaactgctc attgataaaa
     5041 gaaccggtat tattaatggt ttgcaaaaag tggatattga acctgtttta ccgaggaaag
     5101 ccgtctcatg gagagcgcaa gtcaccaatg gaaggatcct tgctaatatt ccgtcagatc
     5161 gcattgcggg gggcactcat ttttccaatg atcaagcaag gaatgcagca atcggggaag
     5221 cgattgagcg atattccgga aattataaag gagggccctt tattcgaggc tcctatcagg
     5281 agttgaaaag aaagggctac aagctgctgg accctgaccg gattcctctt tattctactt
     5341 ggcagtatgc tgaaaaaggt tttcctttta tcccgtttac aaaggattta aaagtgaatt
     5401 gggtacaagg tttttctctt acatgtaaag aagaggtgct gctgcccgcc tcgcttgttt
     5461 atattaacta ttatgaaggg atatttgcca atgagccgcc tgttcatttt gtgaattatg
     5521 ccggcattgc cagcgggccg accttggaat ttgcacttaa atcaggcctt ttggaactga
     5581 tagagaggga cgcaaccgca atctggtggg gatgcagggg ccagattgat gccattgaca
     5641 taaatgatca tcccgatctt cttaacagtt tggaagcaag agaagaaagt gaaatacgct
     5701 atacttttct tcctttaaca acagatataa atattccagt cattggggcg atgctgtatg
     5761 accctgttcg tcaaattgca tgtatgggtt tttcgagcag gcccgatccg aaaaaagcct
     5821 tgctgaaggc tgctgcagaa acggttcaat cctatcattt atcattggat ttactggatc
     5881 cggatagccc gttatgggac gcggttcata caggaatact gcatcaggct gcactgcatt
     5941 cctatcgaaa agacaggaaa tatgttcagt catttaaatc ggattaccat gatatgattg
     6001 atcttttgca taatagccag tattatttag atcctgactc acatcaggat attcgccata
     6061 tctttgatgc agaagaaaag ggaatgcttc cggaaaagca gtctcttact tcatcagata
     6121 ttgcagaggc tctggcaaaa catcaaatcg agtgttttta tgcagatgtg acgactgaag
     6181 atgtcagaac tgccggtttc acagtaacac gagtgatcgc tcccgggctt gtgcctaatg
     6241 caccggcagc ctttccatac cttgcgccta aacgtctgta tgaagtgccg taccgcatag
     6301 ggtggtcaga acatgtgctg gacgagcatc atatcaattt gaagcccatt cctcacagct
     6361 aatcatgaag agggatgata ttgaccttta taaaaagagg tgtttttcat tgcctttttt
     6421 atgtgagacc gcccctgtca gaacctatgc gcgaaatcaa atcgttcctt tggaaaacag
     6481 ccggatttac attgttcaat caggccttgt ttttatatgg gaaacacatc ttgacggacg
     6541 tcaagttttg gtgaatatcc tggacggaaa ttcggccatt caaattcata actttgtcca
     6601 tgcctcatat gaaatgcaaa tcaaaaaaac gtcctttctg caaattacat ttcttgaagc
     6661 gattgataat caaatgaata tactccatgc attgagaaaa caaacattta tgtcagaaga
     6721 gcttgcagcg atccacaggg aaaaatatgt agagcatagg ctgtattttt ttcttcaatt
     6781 tctaaaaaga cattttggaa aggaaatcaa aaaagagcat tatttaatag cagtgcctat
     6841 cacacatgaa gagcttgcat ccgctgtaaa ttcaacaagg ccgaccatta ccaaattgtt
     6901 aaccaaaatg attgataaaa atcaggttgt ctatcagcaa aacgggaaaa atcgctggat
     6961 catattaaag ccgctgttct tttctgctgc agagccgttc taaagataaa aaaaaagctg
     7021 cgtcaacttc aaattgacgg cagctttttt tatcttttat taattttctt cgttgtcttt
     7081 ttcttcttcc caaacgaaag gattgtgaac cagtgcatga tggtgtggat ctacagcaaa
     7141 tgttctgcct tcatctaaaa cttccaataa cgttttgctg gcttctgaaa cttgtttgct
     7201 catttttctc cacctctttg aaaaattttt gagattgcat gcatgcttga tctcatcttt
     7261 attttctttc aattggcaac gtatttctgt cagatcaatg acgacagaat ggtatggaga
     7321 aacacagctt tgacagcgaa ttttatttaa gatgatagaa aaaagggtga tctgatgaca
     7381 cactatatat ttcctcatca tatgattccg tttctaggtt ccgatcaaca agcctatttg
     7441 atgaatgaag aaggagaaat catgaaagta ggatttctga atgaacgttc taacatgtac
     7501 tggggacgtt taaaacctgg cgcttcgatt caattgccgg atgaagagac agaactgatc
     7561 catgtcatga atgaaatttc tcaaagagag gaattcatat ctccctctat tcatatggtt
     7621 actgacgatt ctttttcaaa aaagcttgag gcgaaatttg aacaatccgg attttttata
     7681 tcgtcgacaa accaaaaggg gattcacatt gtattcagcg gatttttcaa ttgcgattct
     7741 tttcaaagat tcagtgatga tgcccaagaa aaccagctgc attggctgcc gctttatttc
     7801 gacggacgta ttgccagagt aggtccattg cttggtcctt atcttcaatt tgaagattta
     7861 aggaagcggc tattagcggc agcatatttt cccgatgtca ctgattcttt atggaaggca
     7921 gcgcaaacgg ctcccttttc agaacatcac ctccctcttg tccatctgga atggctgctg
     7981 acgcaaatca gctggatcgt tcatgattac agctctgcaa atcaactccg gctgcatatg
     8041 catcagatca gccttgaccc cgcaaacatg gaaacaaaac gccatccgat attaaaagtg
     8101 ccgtctccta ttccaagtca tgaaagaaag gatgaatgtc atgtccgcta atgtgctggg
     8161 acttctcgga agtctccgct atccgtcaaa aacagaagaa gctttaagga tatgcctgaa
     8221 ggccgcagag cgcagaggtt gtaccacaag tatatttgat ggcaggatca cgccgcttga
     8281 attttgtgac ggacgcgatg aagaggatac atatgtggaa ggggtggctt tgttgaggga
     8341 aaaagtgcgg gaagcggatg caattgtcat cgcgtctccg gaatacagcg gaagctattc
     8401 tgcttttgcc aaaaatatga ttgattttct cgggccggat tatctgcagg gaaaatgggc
     8461 gggtgttata ggggtggcag ccggaaattc agcggccaat acggtagcgc atctttcagc
     8521 aattcttgca cgaaacggat gctggctcgt ccccacccct gtagaactgc cggcaagcca
     8581 taccatcttt tcacagcacg cgtcttcgcc gaccgcaaag catttggtaa gtttgctgaa
     8641 tgcactgggc gaaaatatcg ctttcagcgt taaccaaata aatcggaggc aatatgaaaa
     8701 ccatacaagt cagtgagacc gttatgttaa gagaattcaa gacagaagat gctgagagcc
     8761 tgtttgagtt gattgatcga tccagaaatc atttgataga atggcttcct tgggttgacg
     8821 gggtaaaaag tgtgaaagac agtcaagggt tcattacgtt tgcacaagaa gaagcggtat
     8881 ccggatcttc gctacatctc ggcatttttg aaaaaggccg tttggcaggg aaagtgtgct
     8941 tatatcaaat caatgacaga atcaaaacag cctctattgg ctactatata ggtgcggatt
     9001 ttgagggaag aggactcgtg acacaagctg taaaacagct ggctgctttt gcctttcaga
     9061 cgcgagggct aaatcgctta gaaataaaaa tgatcagcca aaataaacga agcctcgctg
     9121 tacctcagag acttcgtttc tcttatgagg gctgtttgcg agaaagcgga gaatttcagg
     9181 gaaagcctgc tgaccagctt atattttcgt tgctaaaaag ggaatttcaa tcataaggat
     9241 gcggaaaaag aaagatttta gctaacgttt aaaaatgacc acgacaacaa ttgactgaag
     9301 tgccggcagt gctgggatta aaacgagttt tagagaagcg tggaaatgaa gatgctccga
     9361 gtttgaatga agcctgagtt ttttgaatgg ccttgtgctt tgacaaaaaa gggtgaacta
     9421 agctaagcta caatccaaat cgtaatgttt aagatttata tagggggaaa gacttcttat
     9481 gaagcataaa cggcgaattg gattattgat agtagcttct gtatctctgg ttggcacaat
     9541 ggcagcttgt tcaaagtcaa aagctgaaat ggagcaggtg aacgcggaag taaaccagcc
     9601 tgctgatacg tcatcaacag aaacatcgaa gaatgtaaaa acaaaaacgg agaaaaaaga
     9661 aatcgactgg acaaaacctt cagacggaaa ataccctgac attaaaaata atgattccat
     9721 ctggattgat gtgtctacaa aaaaacagga ggcctacata aaagatggaa gtaaaacaat
     9781 ctatacgatg aatatttcgt caggtctcga tcaaacagag gatgattcaa caccaaaggg
     9841 tacatattat gtagagcctg aacgaggtga gtggtttttc tccccaaaat atcaggaagg
     9901 tgcagagtat tgggtttcat ggaaaaatca tggtgaattc ttatttcata gcgtaccatt
     9961 caacaaagat aaaaaagtca ttgaaaaaga agcaaaaaaa ttagggaaaa aagcttccca
    10021 tggatgcatc aggcttgcga ttccagatgc aaaatggatt tatgaaaaca tacctgaaaa
    10081 aacaaaagta gtgatcagct gatttggctc gttttattta tcaagttagg agactctttt
    10141 tttaagagtc ttctttttgc aaatatcggt ggcatccgaa tttgacgcat gaagaggaaa
    10201 ggcggtcaaa aatagacccc tttctgcttt ttagaatgtt tcatataata aacataaatg
    10261 atatgaatga agtgcagagg aaatgtagtt tttaatcaga cttaggaaac aaaccagccg
    10321 ctaaaaaaga tgaggatcat acatggcaac tgatcttttc atgctgatgt actggttatg
    10381 gagggcatta aaacaaataa agttgatttg taatgattag atgaagtgta agagcatgat
    10441 aatggaataa aagaaacgaa tttaatttaa agaaggaagt gaaagcatgg atcttgaaac
    10501 ggttatgaag gaacttgaag cgctgggtac ggaaaatatg aaaaaaagat acataacgaa
    10561 tggtgcacac gaaccgcttt ttggtgtggc gacaggcaaa atgaagccgc tcgcaaagaa
    10621 aatcaagaaa aatcagcctt tggcggagca gctttacgct accgggaact acgatgccat
    10681 gtattttgcc ggcattattg ctgacccaat cacaatgact gaagctgatt tcgagcgctg
    10741 gatcgatgcg gcgtattttt atatgctttc tgattatgtt gttgccgtaa ctttggcaga
    10801 aaccgatttt gcgcaagctg tcgcagataa atggatttca agcggtgaag aactcagaat
    10861 gtcggcaggc tggagctgtt actgctggct tttaggtaat cgatcggacg atgaatttaa
    10921 tgtgaccaaa attgccggta tgcttgatca ggtggaacat gcgattcacg attctccgga
    10981 tcgaacaaaa tcggctatga ataactttat ctacacagtc gggatttcat atttgccgct
    11041 tcatgaaaag gcggttgaaa ccgcaaaagc agtaggcccg gtcgaacttc ggcgggacaa
    11101 gaaaaaaagc agtatcctga acgcttccaa aaatattcaa atggatgttg agaaaggccg
    11161 ccttggtttc aaacggaaat atgtaagatg ttagcacagt ctgcagtgaa atttctaaaa
    11221 ataaataggg agcggaacat ggaagataac gtttttgaac agatggcaaa acgatatgat
    11281 acagaagaaa gaattgaatt agcaaaagtg atcgtgaatg aagtaagaca ggaattgaga
    11341 aatagtcaat caaaatcttt aa
//
