LOCUS       NZ_JAABOK010000042      1199 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Chitinophaga solisilvae strain O9 NODE_42, whole genome shotgun
            sequence.
ACCESSION   NZ_JAABOK010000042
VERSION     NZ_JAABOK010000042.1
KEYWORDS    WGS; RefSeq.
SOURCE      Chitinophaga solisilvae
  ORGANISM  Chitinophaga solisilvae
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Chitinophaga.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Jianli,Z. and Weiwei,P.
  TITLE     Chitinophaga flavus sp. nov., isolated from forest soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Jianli,Z. and Weiwei,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2020) School of Life Science, Beijing Institute of
            Technology, 5 South Zhongguancun Street, Beijing 100081, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 246.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009928525.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 05:41:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,090
            CDSs (total)                      :: 6,009
            Genes (coding)                    :: 6,004
            CDSs (with protein)               :: 6,004
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:41:33
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1199
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAABOK010000042.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1199
                     /collection_date="2013-07-23"
                     /country="China: Yunnan"
                     /db_xref="taxon:1233460"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Chitinophaga solisilvae"
                     /strain="O9"
                     /submitter_seqid="NODE_42"
                     /type_material="type strain of Chitinophaga solisilvae"
     gene            complement(<1..>1199)
                     /locus_tag="GWG06_RS30340"
     region          1..1199
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1199
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1199
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1199](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1199)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..1199)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (10-121). E-value:
                     7.8e-37. Score: 118.8. Matches aSDomain:
                     nrpspksdomains_GWG06_RS30340_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="GWG06_RS30340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_160718177.1"
                     /sec_met_domain="Condensation (E-value: 1.6e-30, bitscore:
                     92.3, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1e-26, bitscore:
                     79.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCWQPELVNPEHRSYWLDQFSDELPVLELPGDYPRPAGRTYTGGR
                     VRRELSPALLSGLQQLSDQQDASLFMSVLSLVNTLLYRYTGQEDIITGSPVSGREHIDL
                     EDQIGFYVNTLALRSRFHGSGSFIDLLSGVRRHTLQAYAHQSYPFDALVEELPLQRDRS
                     RHPLFDVMVVLQQEADYAAVWNNHSDKLKISEYLSDLHLTSKFDLQFTFLPQGDTLVTE
                     LIYNSDIYSSASASQLLAHLEQLLSVILLTPEQPLYQLDYLTAAEKQQQLSFPESGYPA
                     TLTLVDLFEQQVAATPERIAVSFRDQQISYATLNARSNQLARYLQGQGISGNELVAVQL
                     GRKPEQIIGMLGILKAGGAYVPVDPEYPADRISYMLSDSGSRLVVDDAVLEQLAGYSAE
                     "
     CDS_motif       complement(108..149)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GWG06_RS30340_0003"
                     /evalue="2.40E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="GWG06_RS30340"
                     /protein_end="364"
                     /protein_start="350"
                     /score="20.5"
                     /tool="antismash"
                     /translation="LGILKAGGAYVPVD"
     CDS_motif       complement(678..797)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GWG06_RS30340_0002"
                     /evalue="2.50E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="GWG06_RS30340"
                     /protein_end="174"
                     /protein_start="134"
                     /score="58.4"
                     /tool="antismash"
                     /translation="RRHTLQAYAHQSYPFDALVEELPLQRDRSRHPLFDVMVVL"
     aSDomain        complement(837..1169)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GWG06_RS30340_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="7.80E-37"
                     /label="GWG06_RS30340_Condensation_LCL.1"
                     /locus_tag="GWG06_RS30340"
                     /protein_end="121"
                     /protein_start="10"
                     /score="118.8"
                     /specificity="C activity: inactive"
                     /specificity="Active site motif: -------"
                     /tool="antismash"
                     /translation="EHRSYWLDQFSDELPVLELPGDYPRPAGRTYTGGRVRRELSPALL
                     SGLQQLSDQQDASLFMSVLSLVNTLLYRYTGQEDIITGSPVSGREHIDLEDQIGFYVNT
                     LALRSRF"
     CDS_motif       complement(846..974)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GWG06_RS30340_0001"
                     /evalue="2.00E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="GWG06_RS30340"
                     /protein_end="118"
                     /protein_start="75"
                     /score="42.7"
                     /tool="antismash"
                     /translation="LVNTLLYRYTGQEDIITGSPVSGREHIDLEDQIGFYVNTLALR"
ORIGIN
        1 ttttctgcgc tgtagccggc gagttgttcc agcacggcat catctactac caggcggctg
       61 ccgctgtcgg acagcatgta gctgatgcgg tctgccgggt attcagggtc taccggcaca
      121 tatgccccgc ctgctttcag gatgcccagc atcccgatga tctgttcagg cttacgcccc
      181 agctgcacgg cgaccagctc attgccactg atgccctgcc cctgcaggta gcgcgccagc
      241 tggttagagc gggcattcag cgtagcgtag ctgatttgct gatctctaaa ggaaacggct
      301 atacgttccg gcgtagctgc cacctgctgc tcaaaaagat ctaccagcgt caatgtagcg
      361 gggtagccac tttcagggaa agatagctgc tgttgttttt ctgctgccgt taaataatcc
      421 agctggtaca acggttgttc cggagttagc aatatgacgg acaacagttg ttccaggtgt
      481 gccagcagct gcgaggcgct ggccgaggaa taaatatcac tgttatagat cagctctgtc
      541 accagcgtat cgccctgcgg taaaaaggta aactgcagat cgaatttact ggtcaggtgc
      601 agatccgaaa ggtattcgct aatcttcagc ttatcggaat gattgttcca taccgctgca
      661 taatctgcct cctgctgtaa taccaccatt acgtcaaaca gcgggtggcg gctcctgtca
      721 cgctgcagtg gcagttcttc caccagcgcg tcaaaaggat acgactggtg cgcatatgcc
      781 tgcagggtgt gtctgcgcac accggataac aggtcaatga aactcccgct gccatggaag
      841 cggctgcgca gcgccaacgt gttcacataa aaaccgatct ggtcttccag gtctatatgc
      901 tcacgccctg acacagggct gcccgtaata atatcttcct ggccggtata gcgatacagc
      961 aaggtgttta ccagtgacaa tacactcatg aacagggaag catcctgctg atctgacagc
     1021 tgctgtaaac cagataacaa cgcaggagag agttcccgtc ttacgcggcc gccggtatag
     1081 gtgcggcctg cagggcgcgg ataatcgccg ggaagttcca ggacaggcag ttcatcactg
     1141 aactgatcca gccagtagct gcgatgttcc gggtttacca gctccggttg ccagcacgc
//
