LOCUS       NZ_CP047144            11281 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. HF10 chromosome, complete genome.
ACCESSION   NZ_CP047144
VERSION     NZ_CP047144.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. HF10
  ORGANISM  Streptomyces sp. HF10
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7814787)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7814787)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-NOV-2018
            Assembly Method                   :: SPAdes v. NOV-2018
            Assembly Name                     :: SDU_Sthf_5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:29:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,091
            CDSs (total)                      :: 6,988
            Genes (coding)                    :: 6,640
            CDSs (with protein)               :: 6,640
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 356
            CDSs (without protein)            :: 348
            Pseudo Genes (ambiguous residues) :: 0 of 356
            Pseudo Genes (frameshifted)       :: 235 of 356
            Pseudo Genes (incomplete)         :: 154 of 356
            Pseudo Genes (internal stop)      :: 18 of 356
            Pseudo Genes (multiple problems)  :: 53 of 356
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:39:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6201060
            Orig. end                         :: 6212341
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11281
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11281
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11281
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6281](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6281)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(108..121)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.16"
                     /tool="antismash"
     gene            207..1661
                     /gene="hemG"
                     /locus_tag="GR129_RS27500"
                     /old_locus_tag="GR129_27500"
     CDS             207..1661
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981981.1"
                     /locus_tag="GR129_RS27500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_27500"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_159705993.1"
                     /transl_table=11
                     /translation="MSATGMNAGHAVVIGAGVAGLAAAHRLLERGARVTVLEASERVGG
                     KLLPGEIAGVRVDLGAESMLARRPEAVALAREAGLAERLQPPAIASAAIWTRGALRPMP
                     KGHVMGVPGTAAALAGVLSEDGLARIGRDAVLPRTSVGDDVAVGEYVAARLGREVVDRL
                     VEPLLGGVYAGDAYRISMRSAVPQLFQAARAHASLTEGVREIQAKAAAASQTGPVFMGI
                     EGGVGTLPLAVAESVRARGGEIRTEAPVTELRRDPAGGWRVTAAGQLLHADAVVVAVPA
                     PAAARLLRPESPAAAAELAAVEYASMALITLAYRRAEVSLPEGSGFLVPPVDGRTIKAA
                     TFASQKWGWIADEDPDTLVVRTSVGRYGETAVLGRDDADLVEVSRHDLREAVGIEAAPV
                     ETRVTRWTDGLPQYPVGHHGRVARIREHIARLPGLAVCGAQYDGVGIPACVASANAAVD
                     QLSGDLRGAEELTAHPVQSLHGGAGE"
     gene            1667..2398
                     /gene="hemQ"
                     /locus_tag="GR129_RS27505"
                     /old_locus_tag="GR129_27505"
     CDS             1667..2398
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="GR129_RS27505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_27505"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_159705995.1"
                     /transl_table=11
                     /translation="MSDDAPTTESGRIPNKGKLAKDLNDVIRYTLWSVFKLKDVLPADR
                     AGYADEVQELFDQLAAKDVTIRGTYDLSGLRADADVMIWWHAETSDQLQEAYSLFRRTR
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGFPDVRANTVASFSLGDYEWILAFEANDLSRIVDLMRHLRGSEARMHVR
                     EEVPFYTGRRKDVADLVAGLA"
     gene            complement(2402..4018)
                     /locus_tag="GR129_RS27510"
                     /old_locus_tag="GR129_27510"
     CDS             complement(2402..4018)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (118-491). E-value:
                     2.9e-16. Score: 51.7. Matches aSDomain:
                     nrpspksdomains_GR129_RS27510_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547189.1"
                     /locus_tag="GR129_RS27510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_27510"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_159705997.1"
                     /sec_met_domain="PF00561 (E-value: 1.4e-19, bitscore: 69.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAVAVCSAAVSLVLTALATAPVDATPSRTAAPFPSGAAELRGTV
                     VAALRARATGIRYGACDPADVPAVPHSPAGLRCGTVSVPLDYAHPDGKRIALTVSRAPA
                     TGEDGGRKVPRQGALVYDPGGPGASGMYFALAGTLPVWKRIAAAYDLVGYAPRGVGRSA
                     PLSCEDPKEFGKAPTLAPTRPSEAYKRRRVAEAKAYARGCARRSGPGLRFFNSLNNARD
                     LDVLRAALGEDRLTFMGASYGTYYGSLYAAMFPSQVRRMVLDAPVDPDPQQVWYRSNLD
                     QSAAFEKRWADVRDWIARHDDVYRLGDTPRKVQGSYDTAAARLARKPAGGKVGPGQLQN
                     AFLTAGYYDAYWPGRAAALSAYLRGDPKPLVRLAAPDPGSAAAAENGSAVYTAVECNDA
                     PWPTRWAVWDRDNTRLARVAPFETWDNAWMNLPCAYWPAPRQRPLDVRTAPGALPPVLL
                     LAAERDAAAPYAGALEMNRRLAGSLLVTERGAGNHGIGGGPDKCVDDYMDDYLLTGRLP
                     ARNASCAPRAEPVPGQGRKAG"
     aSDomain        complement(2546..3664)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR129_RS27510_Abhydrolase_1.1"
                     /evalue="2.90E-16"
                     /label="GR129_RS27510_Abhydrolase_1.1"
                     /locus_tag="GR129_RS27510"
                     /protein_end="491"
                     /protein_start="118"
                     /score="51.7"
                     /tool="antismash"
                     /translation="LVYDPGGPGASGMYFALAGTLPVWKRIAAAYDLVGYAPRGVGRSA
                     PLSCEDPKEFGKAPTLAPTRPSEAYKRRRVAEAKAYARGCARRSGPGLRFFNSLNNARD
                     LDVLRAALGEDRLTFMGASYGTYYGSLYAAMFPSQVRRMVLDAPVDPDPQQVWYRSNLD
                     QSAAFEKRWADVRDWIARHDDVYRLGDTPRKVQGSYDTAAARLARKPAGGKVGPGQLQN
                     AFLTAGYYDAYWPGRAAALSAYLRGDPKPLVRLAAPDPGSAAAAENGSAVYTAVECNDA
                     PWPTRWAVWDRDNTRLARVAPFETWDNAWMNLPCAYWPAPRQRPLDVRTAPGALPPVLL
                     LAAERDAAAPYAGALEMNRRLAGSLLVTERGAG"
     gene            complement(4127..4903)
                     /locus_tag="GR129_RS27515"
                     /old_locus_tag="GR129_27515"
     CDS             complement(4127..4903)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675656.1"
                     /locus_tag="GR129_RS27515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_27515"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_159705999.1"
                     /transl_table=11
                     /translation="MFWLLLLLPAWAFTATACVRLCLAAMRAAALDPGPGAAAGDLDLT
                     LYEAAFLSGGPQRVADVTLLSMARQRRLHLAHTGWATVVDPLGRDEIERSVIGAIGPDG
                     QSRIAPVRIRAARTDAVRRLADRLVRSGLAVPDAAGPTIGAAVRQVRAAALAVPVLAVA
                     ALLLPLQADTSRLMVGLWFLLPFTLSLSCLAIARFEVHPYSPWASPAGQRVLGALRRRP
                     GAEPSFLTSVALRGIRAIGEPELRAAFAHRDAPHRE"
     gene            complement(5001..6281)
                     /locus_tag="GR129_RS27520"
                     /old_locus_tag="GR129_27520"
     CDS             complement(5001..6281)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327585.1"
                     /locus_tag="GR129_RS27520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_27520"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_159706001.1"
                     /sec_met_domain="DUF692 (E-value: 2.7e-97, bitscore: 323.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRLGTGIGWRPEIAEAVERMPGIDWVEVVAENVCPGHLPESLGR
                     LRERGVTVVPHGVSLGLGGAQRPDAGRLAALAERAQTLGSPLVSEHIAFVRAGGALTAS
                     PLLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALFSWPDEELTEGQFLY
                     ELAERTGVRLLIDVANLHTNHVNRGEDPAKALDGLPLEAIAYVHVAGGVERDGVWHDSH
                     AHPVPDAVLGILADLAARVAPPGVLLERDENFPEPAELERELGAIRKVVEGGRRGDVEA
                     RAVSPVGEHPGGTEEARQRLGVAQAAVLSSLVARTPVPEGFDRVRLGVQARALAAKRAD
                     VVAKVAPELPVLLGASYREAFLEYARRRPMRGGYRRDALDFAGFLLDGERPGLPRRELR
                     RWWREREGPVRRGRVARAARRVLPGRS"
     misc_feature    complement(6355..6364)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(6418..7689)
                     /locus_tag="GR129_RS27525"
                     /old_locus_tag="GR129_27525"
     CDS             complement(6418..7689)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019330849.1"
                     /locus_tag="GR129_RS27525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_27525"
                     /product="MFS transporter"
                     /protein_id="WP_159706003.1"
                     /transl_table=11
                     /translation="METATGSPKASKPPEAPKTLESPLRLPAFRRFVAANVISASGSAM
                     APLALAYSVIEEGGGAGALGLVLAVNTVPTILFLLMGGLFADRWSRSRILFLGNLAAAG
                     AQGVLAVTVATGHATTVSIAVCGFASGTAASFIVPATQGAVAQIVPGEHLQQANALLRL
                     PGNAVKVLGPVVGGFVVALSGAAWALAWDACTFAVAAVLLLGLRLAVPPAAARGVLADL
                     RAGWAGFRSRTWLWTYTAAGTVLVAAWLAGFQLLGPLVAARRYAGARDWGLVQAAFSCG
                     LLAGTVVCLRWKPYRLLAVAVAAGGSLALPLAAMAWGMALPFVLGAAFLAGIGLDVAVV
                     AWTTAFQQHVPSAEQGRMSAFNGVGERLAIPLGYLLTALAVRAWSSQGVLLACAALIVA
                     TTVLNLCVPDLHRVDRVPREAGPA"
     gene            complement(7761..8210)
                     /locus_tag="GR129_RS27530"
                     /old_locus_tag="GR129_27530"
     CDS             complement(7761..8210)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GR129_RS27530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GR129_27530"
                     /product="hypothetical protein"
                     /protein_id="WP_159706005.1"
                     /transl_table=11
                     /translation="MRLRDARDQIEREHPELTGTAKIEAIKALRTKAAASAVTEPEPVV
                     CRRHCGRCGQDMKPVEKHGWSRFLSWLALLEFGSVIAATAAAVTTVNPDSGGGISILVL
                     WPAAVHPAWLGIVAAIAAFIVTAGLAGSAGRRAAAGTACPECARR"
     gene            8545..8958
                     /locus_tag="GR129_RS27535"
                     /old_locus_tag="GR129_27535"
     CDS             8545..8958
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GR129_RS27535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GR129_27535"
                     /product="hypothetical protein"
                     /protein_id="WP_159706007.1"
                     /transl_table=11
                     /translation="MVDTSPGETYAEIAAATAALTTVRNQWARHPQWPEPLPEKRGRCL
                     VFDPAAVNRFVTEHLDRQAADLEPVRLYTAREIEQLTGITAATIRADRSRGREHRSRLE
                     SACRRVVDRSLSAGRRARQRVTDARLMRPTADS"
     misc_feature    complement(8977..8991)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.95"
                     /tool="antismash"
     misc_feature    9396..9414
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.65"
                     /tool="antismash"
     misc_feature    complement(9400..9418)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.19"
                     /tool="antismash"
     misc_feature    complement(9530..9544)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.0"
                     /tool="antismash"
     gene            9802..9954
                     /locus_tag="GR129_RS27540"
                     /old_locus_tag="GR129_27540"
     CDS             9802..9954
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GR129_RS27540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GR129_27540"
                     /product="hypothetical protein"
                     /protein_id="WP_159706009.1"
                     /transl_table=11
                     /translation="MPKKQKAAVAMIAALAAQAYLSKVAKQQGAALGLPLLAVGAIGYV
                     ISAAL"
     misc_feature    9816..9825
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            10072..10311
                     /locus_tag="GR129_RS27545"
                     /old_locus_tag="GR129_27545"
     CDS             10072..10311
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GR129_RS27545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GR129_27545"
                     /product="hypothetical protein"
                     /protein_id="WP_159706011.1"
                     /transl_table=11
                     /translation="MPLNQETSNAKAGAQAALFNAITEAVAALQTNGGSAARTEGLKNL
                     AEAYAWLIYPNQSHWPTAVQASTSSHFRAARRSP"
     misc_feature    10423..10438
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.59"
                     /tool="antismash"
     misc_feature    10949..10959
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
ORIGIN
        1 acgccacgat ctccagctcc ccgggccccc gctgccgccg cgcctgcgac gccgccgaca
       61 tgcccgtggc gtccccgccg acgacgacca gccgctcccg cccctgatgc atgctcatgg
      121 ggacacgcta gagggtcccg gcccgacgcg gcgcgggacc tacggccctg gcggcgctgt
      181 cagtggggtc tgagagagtg gggaccatga gcgcaacggg tatgaacgcc gggcacgccg
      241 tcgtcatcgg agccggtgtc gccggtctgg ccgccgccca ccggctgctg gagcgcggcg
      301 cgcgggtgac ggtgctggag gcgtccgagc gggtcggcgg caagctgctg cccggcgaga
      361 tcgcgggcgt ccgggtcgac ctcggcgccg agtcgatgct cgcccgccgc cccgaggcag
      421 tggccctcgc ccgcgaggcc ggcctcgccg agcggctcca gcccccggcg atcgcgagcg
      481 ccgccatctg gacccggggc gcgctgcgcc ccatgcccaa gggccatgtg atgggcgtgc
      541 ccggcaccgc cgccgccctc gccggcgtcc tgtccgagga cggcctggcc cgcatcggcc
      601 gggacgccgt cctgccccgc acctccgtcg gcgacgacgt ggcggtgggg gagtacgtgg
      661 cggcgcgcct cggccgcgag gtcgtggacc gcctcgtgga acccctgctc ggcggggtgt
      721 acgcgggcga cgcgtaccgc atctcgatgc gctccgccgt cccccagctc ttccaggccg
      781 cccgcgcgca cgcctccctg accgagggcg tccgcgagat ccaggccaag gccgccgcgg
      841 cttcgcagac cgggcccgtg ttcatgggca tcgagggcgg cgtgggcacc ctgccgctcg
      901 cggtcgccga gtccgtccgg gcgcgcggcg gcgagatccg caccgaggca cccgtcaccg
      961 agctgcgccg ggaccccgcg ggcggctggc gggtcaccgc ggccggacag ctgctgcacg
     1021 ccgacgccgt ggtggtcgcc gtacccgccc cggccgccgc ccggctgctg cgccccgagt
     1081 ccccggcggc cgccgccgag ctggccgccg tcgagtacgc ctcgatggcc ctgatcaccc
     1141 tcgcctaccg gcgcgccgag gtgtccctgc ccgagggcag cggcttcctg gtgccgccgg
     1201 tcgacggacg caccatcaag gccgccacgt tcgcctccca gaagtggggc tggatcgccg
     1261 acgaggaccc ggacacgctc gtcgtgcgca cctcggtggg gcggtacggc gagacggcgg
     1321 tcctcggacg cgacgacgcc gacctggtgg aggtctcccg gcacgacctc cgcgaggcgg
     1381 tcggcatcga ggccgcgccc gtcgagaccc gggtcacccg ctggaccgac gggctgcccc
     1441 agtaccccgt cgggcaccac ggcagggtgg cccgcatccg cgagcacatc gccaggcttc
     1501 cgggcctcgc ggtctgcggc gcgcagtacg acggcgtggg catccccgcg tgcgtcgcga
     1561 gcgcgaacgc ggccgtcgac cagctgtccg gtgatctgcg cggggccgag gagctgacgg
     1621 cccacccggt gcagagcctc cacggcggag caggagaata gggaccatga gtgacgacgc
     1681 ccccaccacc gagtccggca ggatccccaa caagggcaag ctggccaagg acctgaacga
     1741 tgtcatccgc tacaccctgt ggtccgtctt caagctgaag gacgtgctgc cggccgaccg
     1801 cgcgggctac gccgacgagg tccaggagct gttcgaccag ctcgccgcca aggacgtgac
     1861 gatccgcggc acgtacgacc tgtccggtct gcgggcggac gccgatgtga tgatctggtg
     1921 gcacgcggag accagcgacc agctccagga ggcgtacagc ctcttccgcc gcaccaggct
     1981 gggccgcgcg ctggagccgg tgtggtccaa catggcgctg caccgcccgg ccgagttcaa
     2041 ccgctcgcac atcccggcgt tcctcgccga cgagacgccc cgcgactatg tgagcgtcta
     2101 ccccttcgtg cgctcgtacg actggtacct gctgcccgac gaggaccgcc gccgcatgct
     2161 cgccgaccac ggcaagatgg cccgcggctt cccggacgtg cgcgccaaca cggtcgcctc
     2221 gttctcgctg ggcgactacg agtggatcct cgccttcgag gcgaacgatc tgtcccgcat
     2281 cgtcgacctc atgcgccatc tgcgcggctc cgaggcccgg atgcacgtcc gcgaggaggt
     2341 cccgttctac acgggccgcc gcaaggacgt ggcggatctg gtggcgggcc tggcgtaggg
     2401 ctcagcccgc cttccgtccc tggccgggga cgggttcggc gcggggcgcg caggacgcgt
     2461 tccgcgccgg gaggcggccc gtcagcaggt agtcgtccat gtagtcgtcg acgcacttgt
     2521 cggggccgcc gccgatgccg tgattgcccg cgccgcgctc ggtcaccagc agggagccgg
     2581 ccagccgccg gttcatctcc agggcccccg cgtacggcgc ggccgcgtcc cgctcggccg
     2641 ccaggagcag caccgggggc agcgcgccgg gcgcggtgcg cacgtccagg ggccgctgcc
     2701 ggggcgccgg ccagtacgcg cagggcaggt tcatccacgc gttgtcccag gtctcgaagg
     2761 gcgcgacgcg ggccagccgg gtgttgtccc ggtcccacac cgcccaacgc gtgggccagg
     2821 gcgcgtcgtt gcactcgacg gccgtgtaca cggcgctgcc gttctccgcc gccgcggccg
     2881 acccggggtc cggcgcggcc agtcggacca gcggcttcgg gtcgccgcgc aggtacgcgg
     2941 agagggcggc ggcccggccc ggccagtagg cgtcgtagta cccggccgtc aggaacgcgt
     3001 tctgcaactg gccgggaccg accttcccgc ccgccggctt ccgggcgagc cgcgcggcgg
     3061 cggtgtcgta actgccctgc accttccggg gcgtgtcgcc gagccggtag acgtcgtcgt
     3121 gccgggcgat ccagtcccgc acgtccgccc agcgcttctc gaacgcggcc gactggtcga
     3181 ggttgctgcg gtaccagacc tgctgcggat ccgggtccac cggcgcgtcc agcaccatcc
     3241 ggcgcacctg ggaggggaac atcgccgcgt acagcgagcc gtagtacgtg ccgtacgacg
     3301 cgcccatgaa cgtcagccgg tcctcgccca gcgcggcccg caggacgtcc aggtcccgcg
     3361 cgttgttcag ggagttgaag aagcgcagcc cggggcccga acgccgggcg cagccgcggg
     3421 cgtacgcctt cgcctccgcc acgcgccggc gtttgtacgc ctccgagggc cgtgtcggcg
     3481 cgagcgtggg cgccttgccg aactccttcg ggtcctcgca ggacagcggg gcggagcggc
     3541 ccaccccgcg cggggcgtag ccgaccaggt cgtacgcggc ggcgatccgt ttccagacgg
     3601 gcagggtgcc ggccagcgcg aagtacatgc cggaggcgcc gggaccgccg gggtcgtaga
     3661 cgagggcgcc ctgccggggg accttccggc cgccgtcctc gccggtggcg ggggcgcggc
     3721 tgacggtgag cgcgatccgc ttcccgtcgg ggtgggcgta gtcgaggggg acggagacgg
     3781 tgccgcagcg cagtccggcg ggggagtggg gcacggccgg cacgtcggcg gggtcgcagg
     3841 cgccgtagcg gatgccggtg gcgcgggcgc ggagggcggc gacgacggtg ccccgcagtt
     3901 cggcggcgcc ggaggggaac ggcgcggcgg tacggctcgg cgtggcgtcc accggggcgg
     3961 tggccagggc ggtcagcacc agggagacgg cggccgagca gacggcgacg gctttcattc
     4021 ggcatccctt cggtcaccgc tgcggacacc agggatgttt cgtgccgccg ccggggaagg
     4081 caagcaccgc ccgggcggtg tcggcgccag tgccccgatg cgccccctac tcccggtgcg
     4141 gcgcgtcgcg gtgggcgaag gccgcgcgca gctccggttc gccgatcgcg cggatgccgc
     4201 gcagtgcgac ggaggtgagg aacgacggct cggcaccggg gcgccgccgc agcgccccca
     4261 gcacgcgctg cccggcgggg gaggcccacg gcgagtacgg gtgcacctcg aagcgggcta
     4321 tggccagaca gctcagcgac agggtgaacg gcagcaggaa ccacaggccg accatcaggc
     4381 gcgaggtgtc ggcctggagc ggcagcagca gcgcggcgac ggcgaggacc ggcacggcga
     4441 gcgcggcggc gcggacctgg cgcacggccg cgccgatggt ggggcccgcc gcgtcgggca
     4501 cggcgaggcc gctgcgcacg agacggtcgg cgaggcgtcg gacggcgtcg gtgcgggcgg
     4561 cgcggatgcg gaccggcgcg atacgggact ggccgtcggg gccgatcgcc ccgatgacgg
     4621 accgctcgat ctcgtcccgg ccgagcgggt cgacgacggt ggcccagccg gtgtgggcga
     4681 ggtgcaggcg ccgctggcgg gccatcgaca ggagcgtgac gtcggcgacg cgctggggtc
     4741 cgccggacag gaacgcggcc tcgtacaggg tcaggtcgag gtctccggcc gctgctccgg
     4801 ggcccgggtc cagggccgcg gcgcgcatcg ccgcgagaca gagccggacg caggccgtag
     4861 cggtgaacgc ccaggcgggg agcaggagga ggagccagaa catgacgtgt tttgtatgcc
     4921 agggcggcgg gtgggcgcca ggatttttca catgcggacg gggtgcggcc ggttcggcgc
     4981 gcgccccctt gaggggcggt tcaggaccgt cccggcaaca ccctgcgcgc cgcccgtgcc
     5041 acccggcccc ggcgcaccgg tccctcccgt tcccgccacc accgacgcag ctcgcgcctc
     5101 gggagccccg gccgctcccc gtccagcagg aacccggcga agtccagcgc gtcccgccga
     5161 taccccccgc gcatcggccg ccggcgcgcg tactccagga acgcctcccg gtacgacgcc
     5221 cccagcagca ccggcagctc cggcgccacc ttcgccacca cgtccgcccg cttcgccgcg
     5281 agcgcccgcg cctgcacccc cagccgtacc cggtcgaacc cctcgggcac gggcgtccgg
     5341 gccaccagcg aggacagcac ggcggcctgc gcgaccccga gccgctgacg ggcctcctcg
     5401 gtgccgccgg ggtgctcgcc caccggcgag acggcccgcg cctccacgtc cccccgccgc
     5461 ccgccctcca ccaccttccg gatcgccccc aactcccgct ccagctcggc cggttccggg
     5521 aagttctcgt cgcgctccag caggacgccg ggcggggcga cccgggcggc gaggtcggcc
     5581 aggatgccga gcaccgcgtc cggcaccggg tgcgcgtggc tgtcgtgcca cacgccgtcg
     5641 cgctcgacgc cgcccgcgac atggacgtag gcgatcgcct ccagcggcag cccgtccagc
     5701 gccttcgccg ggtcctcgcc gcggttgacg tggttggtgt gcaggttggc cacgtcgatc
     5761 agcagccgta cgccggtccg ctcggccagc tcgtacagga actgcccctc ggtcagctcc
     5821 tcgtccggcc aggagaacag cgcggcgatg ttctccacgg cgagcggcac cggcagcgcg
     5881 tcctgggcga tgcgcacgtt ctcgcagagc acgtcgagcg cgtcccgggt acgcggcacg
     5941 ggcagcaggt ggcccgcctc cagcagcggc gaggcggtga gcgcgccgcc cgcccggacg
     6001 aacgcgatgt gctcgctcac cagcggcgac ccgagcgtct gagcgcgctc ggcgagcgcc
     6061 gcgagccggc cggcgtcggg ccgctgcgcg ccgccgagcc cgagggagac accgtgcggc
     6121 accacggtca ccccgcgctc ccgcaaccgc cccagcgact cggggagatg gccggggcag
     6181 acgttctccg ccacgacctc cacccagtcg atgccgggca tccgctccac ggcctcggcg
     6241 atctccggcc gccagccgat cccggtcccc agtctcccca tggcctgtcc ccctcctcgc
     6301 cccccggcgc acgcgtcgta catgaccccc ggagcccgcc ccgaaccccc gtccggccgc
     6361 cttcagagca acatttgagg ttcggaccgg gtcgcgggcc ggggttccgg ccgcgcgtca
     6421 ggccggaccg gcttcccggg ggacccggtc gacgcggtgc agatccggga cgcagaggtt
     6481 gagcacggtc gtcgcgacga tcagcgcggc gcaggccagg agcaccccct ggctgctcca
     6541 cgcgcgcacc gcgagggcgg tgaggagata gccgaggggg atggcgagcc gttcgccgac
     6601 accgttgaac gcgctcatgc ggccctgttc cgccgagggc acatgctgct ggaacgcggt
     6661 ggtccaggcg acgaccgcga catcgagccc gatccccgcg aggaaggcgg ccccgagcac
     6721 gaacggcagc gccatgcccc aggccatggc ggccagcggc aacgcgagcg aaccgcccgc
     6781 ggcgacggcg accgcgagca gccggtacgg cttccaccgc aggcacacca cggtgcccgc
     6841 cagcagcccg caggagaacg ccgcctggac caggccccag tcgcgcgccc cggcgtaccg
     6901 gcgggcggcg acgagcgggc cgagcagctg gaagcccgcc agccacgcgg cgacgaggac
     6961 ggtcccggcg gccgtgtagg tccacagcca ggtacgggac cggaaccccg cccacccggc
     7021 ccgcagatcg gccaggacgc cccgggccgc ggcgggcggc acggccaggc gcaggcccag
     7081 cagcaggacg gcggcgacgg cgaacgtgca ggcgtcccag gccagcgccc aggccgcgcc
     7141 gctcagcgcg acgacgaagc cgccgacgac cggacccagc accttcaccg cgttgcccgg
     7201 cagtcgcagc agcgcgttgg cctgctggag gtgctccccg ggaacgatct gcgcgacggc
     7261 accctgggtg gccggcacga tgaacgacgc cgccgtcccc gacgcgaagc cgcacaccgc
     7321 gatcgacacc gtcgtcgcgt gcccggtggc cacggtgacg gccagcaccc cctgcgcgcc
     7381 cgccgccgcg aggttcccca ggaacaggat gcggctgcgc gaccaccggt ccgcgaacag
     7441 gccgcccatg agcaggaaca ggatcgtggg cacggtgttc accgcgagca cgagccccag
     7501 cgccccggcc ccgcccccct cctcgatcac cgagtaggcg agcgcgagcg gcgccatcgc
     7561 ggacccggac gccgagatca cgttcgccgc cacgaaccgc cggaacgcgg ggaggcgcag
     7621 gggcgattcg agggtcttgg gcgcctccgg gggcttcgat gccttcggtg agccggtggc
     7681 cgtctccatg atccccctcg gacttggtgg agcgtgcacc ttaatgcagc cgctgcccca
     7741 cgcgagaccg ttcttcgaag ctaacgacga gcgcactcgg ggcaggccgt gcccgctgcg
     7801 gctcgccgac cggccgaccc ggccaagccc gcggtgacga tgaacgcggc gatcgcggcg
     7861 acgatgccca gccaggccgg atgcacggcg gcgggccaga ggacgagaat gctgatgccg
     7921 ccgccgctgt cggggttcac agtggtgacc gcggcggcgg tcgcggcgat cacagagccg
     7981 aattcgagga gcgcgagcca gctgagaaac ctggaccacc cgtgcttctc gaccggcttc
     8041 atgtcctggc cgcagcgccc acagtggcgg cggcaaacca ccggttccgg ttcggtgacg
     8101 gcggacgcgg ctgccttcgt cctcaacgct ttgatcgcct cgattttggc ggtgccggtc
     8161 agttccgggt gctcccgctc gatctggtcc cgggcatccc tcaaccgcat ctgtcatcac
     8221 cttccggctt cagagatggt cgggcggacc atcccaccgg cccgacatcc ggtcaaggcc
     8281 aagttcggca ggaggtgcgc tcagcgcaac gccggatgat cagccatcac cgtgcacgaa
     8341 cctggagcga tctcggtcaa tcccggtgtc gtatcagtca gcgagggaca cggcgcgcct
     8401 gtcaccgtca tctggagggc cgacacgtga accaccgccc ggctcccaac gtcgactgcg
     8461 ctctgcacca gctgaagcga gggcgcgtcc ccgaaccgcc gcgttgcccc gtcactaacc
     8521 atccacgaag gacggacacc cccattggta gacacgtcgc ccggtgagac gtacgccgag
     8581 atcgctgccg ccacagccgc gctgaccacc gtccgcaacc agtgggcccg ccacccgcag
     8641 tggcccgagc cgctgcccga gaagcgcggc agatgcctcg tcttcgatcc ggccgccgta
     8701 aaccggttcg tcaccgagca cctcgaccgc caggccgccg acctggagcc cgtacggcta
     8761 tacaccgccc gcgagatcga gcagctgacc ggtatcaccg ccgccaccat ccgcgccgac
     8821 cgctcccgag gacgcgagca ccgatcacgg ctggagtcag cttgccgcag agtggtggac
     8881 cgctccctgt cagccggtcg ccgggcacgt cagcgggtga cggacgcacg gctgatgagg
     8941 ccgacggcag attcgtgacg aatctggggg ggaccaaagt ttcggaaagt tgagcgccgc
     9001 gcgaagtcgc aaatctagcc cggttgggtc ggccgccaca tttcccagac accagatttg
     9061 ttggagcgct agggtagaga catcacgcat ctatggatgc gtttgggtgg ggtcgtgcgg
     9121 ttcgtggcac cacctgatgc gggtgacccc tcgtgccttt ggcgtagacg acgaggggcc
     9181 acctttacac cagccagtga ccgtgtcgca gtgcccagac caggagggcg gcaaggacgg
     9241 ctgcaaccgt actggccgtt acctggtcaa gccagcgctg cgcgcggtca ctggtccatc
     9301 ggacgcggaa ccgcacgcgc aaggctcggt aggcatcggc gcgctgatcg cgcgagtccg
     9361 cccacctggg taacagcgcc tcaatgaggc gccgcaggtt gcctacagga cacatgttca
     9421 cttcctccct cacgctccgc gagcagtccg gcgcccacgg cgccgccacg gggaccgtcg
     9481 ccttgctaac tcgtggcgca caagggggag atgaacctgg ttggaagtat gtccgacggt
     9541 gtccagtggt gtctcaggat ccacagaaga cgggcaatca tgtggtcggt gcatgaggcg
     9601 tacgctggcg tcgagccccc ggcagagggc aggcgtaccg ggcggtcgta caccgtcggc
     9661 gtcacgagcg ggccgcgcga acccgtgcgt ctgggagggg cagagccccg gcccgctcgc
     9721 aacttcccgt aatcgcaaag ccgttgtcgg accctcgtgt catccttcct tccaactctg
     9781 tgtggagggg aggtgaagaa catgccgaag aagcagaagg cggccgtggc catgatcgcg
     9841 gcgctggctg cgcaggcgta cctgagcaag gttgccaagc agcagggcgc cgcgctcggc
     9901 ctgccgctcc tggccgtcgg cgcgatcggc tacgtcatca gcgctgctct gtagccgcag
     9961 tcgggccctc gctctgccgg ccgaatcggc gaagccaggg cccaccgcgc gtgctagggc
    10021 cagcttgcca tgcagcacga acgacaccgt gagaaccagc caggagaacg aatgccgctc
    10081 aatcaggaaa ccagcaacgc caaggctggg gcgcaggcgg ccctcttcaa cgccatcacc
    10141 gaggcagtgg ccgcccttca gacgaacggt ggaagcgccg cacgaaccga ggggctgaag
    10201 aacctcgccg aggcgtacgc gtggctcatc tacccgaacc agtcccattg gccgaccgca
    10261 gtccaggcga gcacctcgtc gcacttccgg gcggcgaggc gctcaccgtg atcagtcctc
    10321 gtcgtcgtac agagcagaga acgaccgccg cgccgcgcca cccgtgtagg tcgtcagcta
    10381 ggccagtcct cggcggttag aggtcgcgga tcccgaagcc tttgtacgaa ctggtcgatg
    10441 aagccgtggc caccggcgag cgcggccttg gacgggcacg ggcccatgcc gagcatgcgg
    10501 cggatcaggc gatgcatgcg gccagcgtgc cactgcacat ggccaagccc tcgtcggtga
    10561 cgggagcctg gccgtgcgcg ggcaccaccc cgcgctgctg cgccggaccc gccaagacac
    10621 agcgccagca cggcgaacct accgatgccc ggccccaacg actctccagg actacggact
    10681 ccgggccatc ctgcggaccg tggccgcaca ctggacgcgg gaccagcgcg aacaccaccg
    10741 gacgtgactg ccggcccctc gtcaccaccc ttcaagccgc atgctcgctc ggcatgcagc
    10801 ccaagacgtt ccgtgactgg gcgcgccgcg gcgccctgca cccggccgga tcacggccta
    10861 accccaaccg cggccaggcc cttgccctgt gggacttggc cgacatcgct gacactcctt
    10921 gctccgcctg acccaatgtg tggccagctg caaggtcacg agtggggcaa tgcccgttga
    10981 agctgcatcg aacccgtgtt aactgccatg acagtcaacc attttgggaa ccgagaggaa
    11041 tcgctgcatg gacgagagac cggagatcgc tcccgagttg ccgccgatgc cgaccgctcc
    11101 gccagcgcca cccaggtcca cgcgcagacg cgccgtgctc tggatggtga tcgcggctgt
    11161 gctcatcgcg ggcatctcat ctggagtcac cgtaatgatc atctccggct ctgacagctc
    11221 ggctccggtc tccaagccca agacggtcgc ctctgcatct gcggcacctg ctgaggacga
    11281 c
//
