LOCUS       NZ_CP047144            10342 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. HF10 chromosome, complete genome.
ACCESSION   NZ_CP047144
VERSION     NZ_CP047144.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. HF10
  ORGANISM  Streptomyces sp. HF10
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7814787)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7814787)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-NOV-2018
            Assembly Method                   :: SPAdes v. NOV-2018
            Assembly Name                     :: SDU_Sthf_5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:29:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,091
            CDSs (total)                      :: 6,988
            Genes (coding)                    :: 6,640
            CDSs (with protein)               :: 6,640
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 356
            CDSs (without protein)            :: 348
            Pseudo Genes (ambiguous residues) :: 0 of 356
            Pseudo Genes (frameshifted)       :: 235 of 356
            Pseudo Genes (incomplete)         :: 154 of 356
            Pseudo Genes (internal stop)      :: 18 of 356
            Pseudo Genes (multiple problems)  :: 53 of 356
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:39:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2895815
            Orig. end                         :: 2906157
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10342
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="10"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10342
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10342
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[<5000:5342](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<5001..5342)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            272..1573
                     /gene="hisS"
                     /locus_tag="GR129_RS12655"
                     /old_locus_tag="GR129_12655"
     CDS             272..1573
                     /EC_number="6.1.1.21"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hisS"
                     /gene_functions="other (smcogs) SMCOG1162: threonyl-tRNA
                     synthetase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740639.1"
                     /locus_tag="GR129_RS12655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_12655"
                     /product="histidine--tRNA ligase"
                     /protein_id="WP_145485429.1"
                     /transl_table=11
                     /translation="MSRLLPTAPYRGTRDFLPAEMSVRQQVFSHLYETVERYGYGRYDG
                     PILESAEIYEAKSGQEIADQQLYSLTDRGGRRIALRPEMTPSVARMIAANANSLQFPVR
                     WYSHVNCHRYERPQRGRTREHWQLNVDIFGSDSPNCEIEIFELIHDLMGSLGATRDMWL
                     LRVGDRAILNSILRDVVKVPESAHQQTMALIDRWEKVPAEKLREEAAEFGLDARMFDLL
                     ESTLRSGAEVLTALPDEVRETSTLARVMKSEARDLFVFDPFIVRGLQYYTSTVFEVFDR
                     SPDNNRSLFGGGRYGDLAGLFTNQRIPGIGFGMGDVTLHDFLGTHGLLPEARSEVDVSV
                     IPVDDDLVPASREVARRLRQAGIRTSTTLEPRKLGKELSRADRDGARFAVIVGRDEWQR
                     EAVVLKDLKDGAQHEIGLADVVTHLTERLAAPGA"
     gene            1932..2255
                     /locus_tag="GR129_RS12660"
                     /old_locus_tag="GR129_12660"
     CDS             1932..2255
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GR129_RS12660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GR129_12660"
                     /product="hypothetical protein"
                     /protein_id="WP_159701502.1"
                     /transl_table=11
                     /translation="MNDEKRPTGDELMEGLRKALAADSGWIPALAGPRGPAGVGTGSTL
                     DVLVAQLWKFATAPTTPAHVARPLAHAAEAADAALTTDGATRYDALDAAYACVLQAWQA
                     AAR"
     gene            2485..3444
                     /locus_tag="GR129_RS12665"
                     /old_locus_tag="GR129_12665"
     CDS             2485..3444
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672797.1"
                     /locus_tag="GR129_RS12665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_12665"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_159701504.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1.5e-53, bitscore:
                     179.4, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQGVAVESADWSVLGDAVEVVSLREHLADEDALAA
                     ALAEYDIVVTLRERVAFPGSLLARLPRLRLIVATGMRNTVIDYAAAEKHGVTVCGTQSS
                     GTPPVELTWALLLGLARGIVQENTALRTGGPWQSTVGADLHGARLGVLGLGRIGTSVAR
                     VGLAFGMRVGAWSPRLTEERASAAGVELAPSKEELLTSSDFVSVHVPGGDRTRGLIGAA
                     ELALLKPTAYLINTSRASVVDQDALLAALHEGRIAGAGVDVFDIEPLPAGHPMRTAPRL
                     LATPHLGYVSAANYRTYFTQAVEDIQSYLAKSPVRQLP"
     gene            3584..3862
                     /locus_tag="GR129_RS12670"
                     /old_locus_tag="GR129_12670"
     CDS             3584..3862
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136070.1"
                     /locus_tag="GR129_RS12670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_12670"
                     /product="chaplin"
                     /protein_id="WP_145485426.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVALGTGAVVIGSAGLALADAGAEGAAVGSPGVLSGN
                     VIQVPVNIPVNVCGDSIDVIGLLNPAFGNTCVNQGGALARPSADYGD"
     gene            complement(3992..4858)
                     /locus_tag="GR129_RS12675"
                     /old_locus_tag="GR129_12675"
     CDS             complement(3992..4858)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="GR129_RS12675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_12675"
                     /product="tyrosinase family protein"
                     /protein_id="WP_145485425.1"
                     /transl_table=11
                     /translation="MAYVRKNVGALTATERKRFVDALLEVKRRGQYDEFVRTHIAYFAA
                     DGETHLRAAHMAPSFLPWHRRFLLDLENALRRVDSSVTLPYWDWTRDRTATSVPWTKDL
                     LGGNGRSSDQQVTTGPFAYSTGNWTIREGVTDGRYLTRDLGRAASPLQLPTKDDVNWAL
                     NDPVYDVRPWDSTVTQGFRNKLEGWGTGSGSASWHNHNRVHRWVGGAMLGAASVNDPVF
                     WLHHAYLDMQWERWQRAHSSHRYLPADPPGAGDAQHGRVVARHEKLPPWSDTPDQLEDM
                     SGVYRYA"
     gene            complement(<5001..5342)
                     /locus_tag="GR129_RS35885"
                     /pseudo=""
     CDS             complement(<5001..5342)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545036.1"
                     /locus_tag="GR129_RS35885"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="tyrosinase cofactor"
                     /pseudo=""
                     /sec_met_domain="melC (E-value: 1.3e-44, bitscore: 150.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRGLLSCVAALALAPVVAASRPVPPAAGGDSFDETYRGRRIQGV
                     RLPAAGRSTADEAWRITVDGRPLHLMRRADGTWLSMVDHYTSYRTPQEAARAAVDELGP
                     GQRLSDPAPG"
     gene            complement(5531..6034)
                     /locus_tag="GR129_RS12685"
                     /old_locus_tag="GR129_12685"
     CDS             complement(5531..6034)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="GR129_RS12685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_12685"
                     /product="hypothetical protein"
                     /protein_id="WP_145485423.1"
                     /transl_table=11
                     /translation="MKPTTRGTLAAVLTGVAAAASATAPAAAAGAIPVPVPLQGVSHSL
                     GTRMPEATLEVPLLTPGAPEGPRYVTGRLLPEPILPRLPIGGTLPGADLRAPLPHPLGD
                     RLGVDAPAAGLHTAGPGLNLVAPLTAPDPHLFGLPAPRLPEAGLLAPALRTVAGGDLGV
                     GPAR"
     misc_feature    6134..6150
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.24"
                     /tool="antismash"
     misc_feature    6277..6292
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.79"
                     /tool="antismash"
     gene            complement(6353..6844)
                     /locus_tag="GR129_RS12690"
                     /old_locus_tag="GR129_12690"
     CDS             complement(6353..6844)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065676.1"
                     /locus_tag="GR129_RS12690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_12690"
                     /product="DUF5949 family protein"
                     /protein_id="WP_145485422.1"
                     /transl_table=11
                     /translation="MTSTTTELRPADLGTLVVLPWSGAAPDGTDMPYLLAYSLGDAAGG
                     PQVTAAAVEQLLVSNGLPVGGDLVDGTYRPSLPITLLVEAGQAVVRMPRLIAQAPAPPE
                     WLAAVRARGFAYLVFTTRAWPEGAPGRIVQPDALAAFAGAPETLHAAAHVVLPATSLRG
                     "
     gene            complement(7001..7291)
                     /locus_tag="GR129_RS12695"
                     /old_locus_tag="GR129_12695"
     CDS             complement(7001..7291)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116485.1"
                     /locus_tag="GR129_RS12695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_12695"
                     /product="hypothetical protein"
                     /protein_id="WP_145485421.1"
                     /transl_table=11
                     /translation="MKSLKAAAVLAGSVALAGVAAPAFAYNASDLTPSSLNGAVDQLAK
                     GDYSLRDLMPLQHQSNALDTEDKDSVLHSVKGATTALNQHNPLLGGLPLKG"
     gene            complement(7419..8174)
                     /locus_tag="GR129_RS12700"
                     /old_locus_tag="GR129_12700"
     CDS             complement(7419..8174)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GR129_RS12700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GR129_12700"
                     /product="hypothetical protein"
                     /protein_id="WP_159701506.1"
                     /transl_table=11
                     /translation="MRALPVRRLASTALSAAVIAAITGPVAVAVDTARGHGEAPASQTA
                     LPGAAKLLARVRTLDRAGALPKPVVELLDRSLTKGRLPAAEAERLGEAAKQAVVRALAA
                     GTAGAQAPGGPTAAAVPAAPVAPAPAAPTAAAPAQAATPASPAAPAKPAPVPSGSESQS
                     ALPEPAASPAVPLSPAPRGAARSVPVARDLVSDVLAAVDALVQAAVSDVDQVVAAADTL
                     VDQLLSALGIGVPTPTPTPTTLPSVPAAG"
     misc_feature    8355..8370
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            complement(8379..8618)
                     /locus_tag="GR129_RS12705"
                     /old_locus_tag="GR129_12705"
     CDS             complement(8379..8618)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568410.1"
                     /locus_tag="GR129_RS12705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_12705"
                     /product="chaplin"
                     /protein_id="WP_111584533.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSAAVAAVAGAAGAAAASSGADGVAANSPGVISGN
                     VIQVPVHIPVNVCGDSIDVIGLLNPAFGNTCVNG"
     gene            complement(8835..9593)
                     /locus_tag="GR129_RS36785"
                     /old_locus_tag="GR129_12710"
     CDS             complement(8835..9593)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672819.1"
                     /locus_tag="GR129_RS36785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_12710"
                     /product="chaplin"
                     /protein_id="WP_145485420.1"
                     /transl_table=11
                     /translation="MRQTLSKGMVVAAAAATGVLSLYGTAALADSNAQSLAQGSPGVLS
                     GNSIQVPVHVPVNVCGNSVDVAALLNPAFGNSCANGGHSATHHPTGYGNDSYGDDEPTP
                     ASYGKPDPTPTPTSYGHDDPTPPAYGSPEPTPTPTSYGSDEPTPTSYGSDEPTPTSYGS
                     DEPTPTPTSYGSDEPTPTPTSYGSDEPTPLPSYGSDEPTPPGSYGNDEPPTLPHTGGNA
                     KAMIATSAASAALIMAGAVLYRRGRTAARR"
     misc_feature    complement(9576..9594)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.04"
                     /tool="antismash"
     gene            complement(9725..9952)
                     /locus_tag="GR129_RS12715"
                     /old_locus_tag="GR129_12715"
     CDS             complement(9725..9952)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140819.1"
                     /locus_tag="GR129_RS12715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR129_12715"
                     /product="chaplin"
                     /protein_id="WP_145485419.1"
                     /transl_table=11
                     /translation="MKKSAAVVAGALLALGGAAPAFADAGAGAVAANSPGVLSGNVIQI
                     PVHVPINLCGDSVDIIGLLNPAFGNTCANG"
ORIGIN
        1 ccctggaacc ggccgaccgg gcgttcgccc ggctgcacga cgtacgggaa cggctgctcg
       61 ccgaccaccc ggacgccgtg gtgatgtcgg ccggcatgag cggggacctg gaggccgcga
      121 tcgccgcggg cgcgacgcac gtcagagtgg gatcggccgt ggtgggaccc cgcacgcccg
      181 cggggactcc cgcaccacaa ctctgacatg acgtcaaccc ccccttgtta caatgccctt
      241 cactacgcgt gacccccata ggagcccctc cgtgagtcgt ctgctgccca ccgctcccta
      301 ccgcggcacc agggatttcc tccctgccga gatgtccgtc cgacagcagg tgttcagcca
      361 tctgtacgag acggtggagc gctacggcta cggccgctac gacgggccga tcctggagtc
      421 cgcggagatc tacgaggcga agtccggcca ggagatcgcc gaccagcagc tgtactccct
      481 caccgaccgc ggcgggcgcc ggatcgcgct gcgcccggag atgaccccct cggtggccag
      541 gatgatcgcg gccaacgcca acagcctcca gttcccggtg cgttggtaca gccacgtcaa
      601 ctgccaccgc tacgagcggc cgcagcgcgg ccgtacccgt gagcactggc agctcaacgt
      661 ggacatcttc gggtccgaca gcccgaactg cgagatcgag atcttcgaac tcatccacga
      721 cctgatgggc tcgctcggcg cgacccgcga catgtggctg ctgcgggtgg gcgaccgcgc
      781 catcctcaac agcatcctgc gcgacgtggt gaaggtgccc gagagcgcgc accagcagac
      841 gatggccctc atcgaccgct gggagaaggt gccggccgag aagctgcgcg aggaggccgc
      901 ggagttcggc ctggacgccc ggatgttcga cctcctggaa tccacgctgc gctccggggc
      961 cgaggtgctg accgccctcc cggacgaggt ccgcgagacc tccacactgg cgcgggtgat
     1021 gaagagcgag gccagggacc tcttcgtctt cgaccccttc atcgtgcgcg gcctgcagta
     1081 ctacacctcc accgtcttcg aggtgttcga ccgctcgccg gacaacaacc gctcgctctt
     1141 cggcggcggc cgctacggcg acctggccgg cctcttcacc aaccagcgca tccccggcat
     1201 cggcttcggg atgggcgacg tcaccctgca cgacttcctc ggcacccacg gcctgctccc
     1261 cgaggcccgc agcgaggtgg acgtcagcgt gatccccgtc gacgacgacc tggtccccgc
     1321 ctcccgcgag gtcgcccgcc ggctcaggca ggccgggatc cgcacctcga ccacgctgga
     1381 gccgcgcaag ctgggcaagg aactctcgcg cgccgaccgc gacggcgccc gcttcgcggt
     1441 catcgtcggg cgcgacgagt ggcagcgcga ggccgtcgtc ctcaaggacc tcaaggacgg
     1501 cgcgcagcac gagatcggac tggccgacgt ggtcacccac ctcacggagc gcctggcggc
     1561 tccgggggcc tgacctcccc gaagccgcaa aagacatagg ggcacccccc ggggaaccgg
     1621 gaaccgggcc cccacccctc cgtgccgaca ccgccgtgaa gccgctcccc ggggccgccg
     1681 gacctctccg acgccccggg gcactccccc ctccccccac gccctccggc gaacacggcc
     1741 acgccgacgg gccgcacgcc gcggtctgcc gaaggagtcc ggccgcgccg gcgctcccgc
     1801 acccctccgc ggccgacaga ggcatgcccg aaatgacccg gcacgtgccc ggcaagcgcg
     1861 ggatgccgcg ttcccccgtc ccgctcgtcc ggccccttct tccctctgca ctcatggaga
     1921 ccgagcctca catgaacgac gagaaacgcc cgaccggcga cgagctgatg gaagggctcc
     1981 gcaaggcgct cgccgccgac agcggctgga tccccgccct cgcgggcccg aggggacctg
     2041 ccggtgtcgg caccggttcg acactggacg tgctcgtggc acagctgtgg aagttcgcga
     2101 cggccccgac cacccccgcg cacgtcgccc gcccgctcgc gcacgcggcc gaggcggccg
     2161 acgccgccct caccacggac ggcgcgacgc ggtacgacgc cctggacgcg gcgtacgcat
     2221 gcgtactcca ggcctggcag gcagcagccc gctgacccgg ccctgagcgg caggcgcaac
     2281 cgccggcccg tcgcaccggt ggccccggcc cacaggccgt ggcggagatc tggtacctcg
     2341 gtcggcgcga cggccacctg gggaacgttc ctgacgccct ggtcgcggtg cggacgcggg
     2401 agtcgttcgg ggagcgcgcc gctcgcccgc cgtccgacat acttcgaggc gctcgaagtg
     2461 ctcggaacgt gaggggagaa cggcgtgcga cttcgctgtg cggtgctgga cgatttccag
     2521 ggggtggccg tcgagtcggc cgactggtcg gtgctcgggg acgcggtgga ggtcgtgtcg
     2581 ctgcgggagc acctggccga cgaggacgcg ctcgccgcgg cgctcgccga gtacgacatc
     2641 gtcgtcaccc tgcgcgaacg cgtcgccttc ccgggctcgt tgctcgcccg gctgccccgg
     2701 ctgcggctga tcgtggcgac cgggatgcgc aacaccgtga tcgactacgc cgcggccgag
     2761 aagcacggtg tcaccgtgtg cgggacgcag agttccggga ctcccccggt ggagctgacc
     2821 tgggcgctgc tgctggggct cgcccggggg atcgtccagg agaacaccgc gctgcgcacc
     2881 ggcggcccct ggcagtccac ggtcggcgcc gatctgcacg gcgcccgtct cggcgtgctc
     2941 gggctcggcc ggatcggcac ctcggtcgcg cgggtcgggc tcgccttcgg gatgcgggtc
     3001 ggcgcgtgga gccctcggct gaccgaggag cgcgcctcgg cggccggggt ggaactggct
     3061 ccttccaagg aggagttgct gacgagcagc gacttcgtct ccgtgcacgt gcccggcggg
     3121 gaccgcaccc gggggctgat cggcgccgcc gaactcgccc tcctgaagcc gaccgcctac
     3181 ctgatcaaca cctcccgcgc ctcggtggtg gaccaggacg cactgctggc cgcgctgcac
     3241 gagggccgga tcgcgggcgc cggcgtcgac gtcttcgaca tcgagcccct gcccgccggc
     3301 caccccatgc gcaccgcgcc ccgcctgctc gccaccccgc atctcggcta tgtctccgcg
     3361 gccaactacc ggacgtactt cacccaggcc gtggaggaca ttcagtccta tctggcgaaa
     3421 tctccggtac gacaattgcc gtgaatccgc cgagaacggg cgtctgtctg ttgaaagggt
     3481 gatcccccgc cgatcaccgt gacccctgtc ggacggccgc gttgaacgtg gagtgcgacg
     3541 gcgggagcag ccgtcgcgaa tcccgagcga caggaggaac gtgatgtctc gcatcgcgaa
     3601 ggcagccgcc gtggccctcg gcaccggtgc cgtggtgatc ggttcggccg ggctggctct
     3661 ggccgacgcg ggcgccgagg gcgcggccgt cggctcgccc ggcgtgctgt ccggcaacgt
     3721 gatccaggtt ccggtcaaca tcccggtcaa cgtctgcggt gacagcatcg acgtcatcgg
     3781 tctgctgaac ccggccttcg gcaacacctg cgtcaaccag ggcggcgccc tcgcgcggcc
     3841 cagcgccgac tacggcgact gacacctcgc ccgcgcaccg cgaagagccc cggcacctcc
     3901 gagcgccggg gctcttcgcg cgcgtcaccg gaccgaaccc cctggcgtac accgacggca
     3961 attcacccgt accggtgacc ccggtgtgag ttcacgcgta ccggtagacc ccgctcatgt
     4021 cctccagctg atccggggtg tcgctccacg gcggcagctt ttcgtgccgg gcgacgaccc
     4081 ggccgtgctg cgcgtcaccg gcgccgggcg gatcggccgg cagatagcgg tggctgctgt
     4141 gcgcccgctg ccagcgctcc cactgcatgt cgaggtaggc gtgatggagc cagaacaccg
     4201 ggtcgttgac cgacgcggcg ccgagcatcg ccccgccgac ccagcggtgc acccggttgt
     4261 ggttgtgcca ggaggcgctg ccgctgccgg tgccccagcc ctccagcttg ttccggaagc
     4321 cctgggtgac ggtcgagtcc cagggccgta cgtcgtagac cgggtcgttc agcgcccagt
     4381 tcacgtcgtc cttcgtgggc agttggaggg gtgaggcggc gcggccgagg tcgcgggtga
     4441 ggtagcggcc gtcggtgacg ccctccctga tggtccagtt gcccgtcgag taggcgaacg
     4501 ggccggtcgt cacctgctgg tcggagctgc ggccgttgcc gccgagcagg tccttggtcc
     4561 agggcaccga ggtcgccgtg cggtcccggg tccagtccca gtacggcagg gtcaccgagg
     4621 agtcgacgcg gcgcagcgcg ttctccaggt ccagcaggaa ccggcggtgc cagggcagga
     4681 aggacggcgc catgtgggcg gcccgcagat gggtctcgcc gtcggcggcg aagtacgcga
     4741 tgtgggtgcg cacgaactcg tcgtactggc cgcgccgctt gacctccagc agggcgtcca
     4801 cgaaccgttt ccgctcggtc gcggtgaggg cgccgacgtt cttacgcacg tacgccatac
     4861 cggccccccg tccccgtccc cgcactcatg tcggtaccgc ccatgccggt accgcccatg
     4921 tccatgccgc ccgtgtccat ggtcatgccc atgcggtcca tcatgtcctt gacgccgtcg
     4981 ggcagcgcgc cctccagcgg gcccggggcc gggtcgctga gccgctggcc gggcccgagt
     5041 tcgtccaccg cggcccgcgc cgcctcctgg ggcgtgcggt acgacgtgta gtggtcgacc
     5101 atgctcagcc aggtgccgtc ggcccgccgc atcagatgca gcgggcggcc gtcgacggtg
     5161 atccgccagg cctcgtcggc cgtgctgcgt ccggcggccg gcagccggac gccctggatg
     5221 cgccggcccc ggtacgtctc gtcgaaggag tcaccgccgg cggccggggg caccggccgg
     5281 gaggcggcga cgaccggcgc gagggcgagg gcggcgacgc aggacagcag cccccgcgtc
     5341 acccgcctgc gcccgaggcc ggccgtcgta tccgctctga cctgctctgt tgcggccgcc
     5401 gcagctgcgt cgtcgccgtc gaccatcgca ctctcctcgt ccgggaacag atgtcgtacc
     5461 cgctaacgga cgaggagggg ccgggtcacc ggcgtacggc cgaacggcgc tggtgaggga
     5521 gccctgcggg ttaccgggcg ggccccacgc ccaggtcgcc gcccgcgacg gtgcgcaggg
     5581 ccggggcgag caggcccgcc tccgggagcc ggggggcggg caggccgaac aggtgcgggt
     5641 cgggcgcggt caggggggcg accaggttca gaccgggtcc ggcggtgtgc agcccggcgg
     5701 cgggggcgtc gacgccgagc cggtcgccga gcggatgcgg cagcggcgcc cgcaggtcgg
     5761 cgccgggcag cgtcccgccg atcggcagcc tcggcaggat cggctcgggc agcagccggc
     5821 cggtgacata gcgcggcccc tcgggcgcgc ccggcgtcag cagcggcacc tccagcgtgg
     5881 cctcgggcat ccgcgtgccg agggagtggg agacgccctg gagcggtacg ggcacgggga
     5941 tcgcgccggc cgcggcggcg ggggcggtcg cgctcgcggc ggccgcgacg ccggtcagca
     6001 cggcggcgag ggttcctcgg gtggtcggct tcatctcggg tacggccctt tccggatgcg
     6061 ggagttcggc gtccgtatcg cgtaacgggc ggtgccggga cggagaacgg ggcggcgcga
     6121 caattccccc ggttgccgta atagtcggca tggacgacat ggttacctgg tcgtcctggt
     6181 gagccggtcg gccgtacccg ccgtcgcccg accggccacg gtcacactca ccggccgatc
     6241 gcgctcgtac gaccggtcgc gctcgtcagc cggtcggtca ccggcggctc acgctcgtac
     6301 ggccccttgt ccgcgccgcc gtatgccggg gccccggccc tcgtccacgc cgtcagccac
     6361 gcagactggt ggccggcagc acgacatgcg cggcggcgtg cagggtctcc ggggcgccgg
     6421 cgaacgcggc cagcgcgtcc ggctggacga tcctgccggg cgcgccctcg ggccacgcgc
     6481 gggtggtgaa caccaggtag gcgaagccgc gggcccggac ggcggcgagc cactcgggcg
     6541 gcgcgggggc ctgcgcgatc agccgcggca tccgtacgac ggcctgcccg gcctccacca
     6601 gcagggtgat ggggaggctg ggccggtagg tgccgtcgac gaggtcgccg ccgaccggca
     6661 gtccgttgct caccagcagc tgctcgaccg cggccgcggt cacctggggg ccgcccgcgg
     6721 cgtcccccag ggagtaggcc agcaggtagg gcatgtcggt gccgtcgggg gccgcgccgc
     6781 tccagggcag cacgaccagc gtgccgaggt cggcggggcg cagttccgtg gtggttgagg
     6841 tcaccgcgcg accataccga cgcgcccggg ggcggcccgg cggcttctca cccgtccgga
     6901 ccagtggacc ggggggctcc acacgaacga gggaaacggc ctggaaaggg gcggggccgg
     6961 taccgcggat gcggtaccgg ccccgggcgc gcggaggatg tcagcccttc aggggcaggc
     7021 cgccgagcag cgggttgtgc tggttcagcg cggtggtggc gcccttgacc gagtgcagca
     7081 cggagtcctt gtcctcggtg tcgagggcgt tcgactggtg ctggagcggc atcaggtccc
     7141 gcagggagta gtcacccttg gcgagctggt cgacggcgcc gttgaggctg gagggcgtca
     7201 ggtcggaggc gttgtaggcg aacgcgggcg cggcgacgcc ggcgagggca acggaaccgg
     7261 ccaggacggc ggcagccttc agggacttca tcgtgttcct ttcttcggca acgacgtgtc
     7321 caacgagcgt cgcggcggag cggaaacccc atggccgaga agacatatga acatgcctcg
     7381 gccggaaggg ggcggggccc ggggccggac tccgcgggtc agcccgccgc gggcacgctc
     7441 ggaagcgtcg tcggggtggg ggtcggggtg ggcacgccga tgccgagggc ggagaggagc
     7501 tggtcgacca gggtgtcggc ggcggcgacc acctggtcga cgtcggagac ggccgcctgc
     7561 accagggcgt ccaccgcggc cagcacatcg ctgacgaggt cacgggcgac cggcacgctg
     7621 cgggccgccc cccgcggggc cgggctgagc ggtacggcgg gcgacgcggc cggctcgggc
     7681 agcgccgact gggactcgga tcccgagggg acgggggcgg gcttggcggg ggcggccggg
     7741 ctcgcggggg tcgcggcctg ggccggagcg gcagccgtcg gagcagcggg cgccggagcg
     7801 acgggcgccg ccgggaccgc ggccgccgtc gggccgcccg gagcctgcgc cccggccgtc
     7861 cccgccgcca acgcccgtac gacggcctgc ttcgccgcct cgccgagccg ctcggcctcc
     7921 gccgcgggca gtcggccctt cgtcagtgag cggtcgagca gttcgacgac cggcttgggc
     7981 agggcgcccg cgcggtccag ggtcctgacc cgggcgagga gtttcgcggc gccgggcagg
     8041 gcggtctggg aggcgggggc ctcgccatgg ccgcgcgccg tgtcgacggc gacggcgacc
     8101 gggccggtga tcgcggcgat gacggcggcg ctcagagcgg tggacgcgag gcgccggacg
     8161 ggcagagcac gcatgcgggt tcctttcggc tgggcttcgg ggtctgagcc caccgtcagt
     8221 tcgccccacc cgccccgcaa ccgctcgcga cgccatgtgc cccgtccggg cccgccactg
     8281 ccccgtccgg gggatgggcg gacatgagaa ccggccgccc acctccgcga cggatgcgcg
     8341 gagtggacgg ccggtcgaag gggtgtgcgc cccggacgtc agccgttgac gcaggtgttg
     8401 ccgaacgccg ggttcagcag accgatgacg tcgatgctgt caccgcagac gttgaccggg
     8461 atgtggaccg ggacctgaat gacattgccc gagatgacac cgggcgagtt ggcggcaacg
     8521 ccgtccgcgc cggaggaggc ggcggcggca ccggcggcgc cggcgacggc cgcgacggcg
     8581 gcggaggtca ggaccagacc cttcgcgata cgcgacatgg agaggtgctc cttggaatag
     8641 acacaaacat ccgggcggat gcccggcgtt gtgtcaacgc gtcgcgctcc cccgggttgt
     8701 gccgccaaag gagtgaagtc cgacggaggc cgctcggaag ggccttgggc gcccatgccg
     8761 aaaacccccg cacgggcgtg cgcgggggtc ctgggcggcg ggtgccggac gctcggggtc
     8821 cggcacccgg caggtcaccg acgggcggcc gtccggccgc ggcggtagag caccgcaccc
     8881 gccatgatca gcgcggcgct cgcggccgag gtcgcgatca tggccttggc gttgccaccg
     8941 gtgtgcggca gggtgggcgg ctcgtcgttg ccgtacgaac cgggcggggt cggctcgtca
     9001 ctgccgtagg agggcagcgg ggtcggctcg tcgctcccgt acgacgtcgg cgtcggcgtc
     9061 ggctcgtcgc ttccgtacga cgtgggggtc ggcgtaggct cgtcgctgcc gtaggaggtc
     9121 ggcgtcggct cgtcgctccc gtaggaggtc ggcgtcggct cgtcgctgcc gtacgacgtc
     9181 ggggtcggcg tcggctccgg gctgccgtac gccggcggtg tggggtcgtc gtggccgtac
     9241 gacgtgggcg tgggagtcgg gtccggcttg ccgtaggagg cgggggtcgg ctcgtcgtcg
     9301 ccgtacgagt cgttgccgta acccgtgggg tggtgggtgg ccgaatggcc gccattggca
     9361 caggagttgc cgaacgcggg gttcagcagc gcggccacgt ccacggagtt gccgcagaca
     9421 ttgaccggca cgtgcaccgg gacctggatg ctgttgccgg acagcacgcc cggtgaaccc
     9481 tgggccagtg actgcgcgtt ggagtcggcg agcgcggccg tgccgtacaa cgaaagcacg
     9541 ccggtcgcgg cagctgcggc cacgaccatg cccttgctga gggtctgtcg caatctcgta
     9601 gtcttcctac tcgaactgct cgaagatgtg ggaaggccgg ccccgggcca atggatctat
     9661 ggatcgacgg tccgaggccg gcctgaacgc agccggccgg ctggcgtgcg gtgggtaccg
     9721 aacgtcagcc gttggcgcag gtgttgccga acgcggggtt cagcagaccg atgatgtcga
     9781 cggagtcgcc gcacaggttg atcggaacgt gcacagggat ctggatcacg ttgccggaca
     9841 gcacgccagg ggagttggcg gcgacggccc cggcaccggc gtcggcgaag gcgggggcgg
     9901 ccccgcccag tgccaggagg gcaccggcga cgactgcagc gctcttcttc atggaatttc
     9961 ccttctctga cggtcatgcc cctatgagcg gtcgaaaccg agcgagcaca gccttgaacg
    10021 gctcgcacca tgaccggcag gttgtaaaac gaggcataga cagccggaga aaccctggaa
    10081 cgtcctgggc cccctaattc actcgaacgc ccccatagaa ttgacacctt ggacgattaa
    10141 cggataggtg cttcgtcggg aagaacgaca aaaccgcccg ggcgccgcac cggaaaatcg
    10201 gtgcggcgcc cggacggcac tcggggtggg acgtgttcag ctgccgacgg cgccgttgcc
    10261 cgccaggacg gagatgtcgt ccgcgatgtg cgagagcggc tcgtcgtcct tcgcctgcgt
    10321 cgagttctcg gcgcactgct gg
//
