LOCUS       NZ_CP047146            10981 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. GS7 chromosome, complete genome.
ACCESSION   NZ_CP047146
VERSION     NZ_CP047146.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GS7
  ORGANISM  Streptomyces sp. GS7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9067183)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9067183)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-NOV-2019
            Assembly Method                   :: SPAdes v. Nov-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:53:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,019
            CDSs (total)                      :: 7,925
            Genes (coding)                    :: 7,616
            CDSs (with protein)               :: 7,616
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 107 of 309
            Pseudo Genes (incomplete)         :: 258 of 309
            Pseudo Genes (internal stop)      :: 29 of 309
            Pseudo Genes (multiple problems)  :: 79 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:40:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6909415
            Orig. end                         :: 6920396
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047146.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10981
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="31"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10981
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10981
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5981](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5981
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            108..989
                     /locus_tag="GR130_RS30020"
                     /old_locus_tag="GR130_30020"
     CDS             108..989
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536997.1"
                     /locus_tag="GR130_RS30020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_30020"
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_159507610.1"
                     /transl_table=11
                     /translation="MSDQQAQQAQHTPRSLIVTFYGAYGRGGPDRPGGAPGPVPVAALI
                     RLLGALGVDPPSVRSAVSRLKRRGLLVAERTAAGAAAYGLSDAARQLLEDGDRRIFGRP
                     AARLSDGWVLAVFSVPEEERHKRHLLRSRLSRLGFGTAAPGVWIAPAHLYEETRHTLER
                     LELGPYVDLFTGTHAGFAPTAQAVARWWDLDAIAARHRAFLAEHEPVLARWARRRSVPP
                     EAAYRDYLLALDAWRRLPYADPGLPSPLLPEDWPGGRAAEVFGRLDAKLRAAGARYACE
                     ATAGPHRTRGTV"
     misc_feature    154..168
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.44"
                     /tool="antismash"
     gene            complement(1089..1859)
                     /locus_tag="GR130_RS30025"
                     /old_locus_tag="GR130_30025"
     CDS             complement(1089..1859)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536998.1"
                     /locus_tag="GR130_RS30025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_30025"
                     /product="HAD family hydrolase"
                     /protein_id="WP_236573659.1"
                     /sec_met_domain="HAD_2 (E-value: 1.8e-27, bitscore: 95.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVSMEVGERPVRGARSAGTETIRAAVFDLDGTLANTLPAISKLL
                     VKVASEQGCSVTARQAAAAIGKPPGAAFGRLLGRPEEDGRVQAAISRYRELFSYEVLCQ
                     GPQLLFPGVTAGLSALRAHGVELAVATSKTTRGAEALLDVMGIRDLVGPVIGQDMVRRG
                     KPHPEMVLRAAGRLGVPAWQSAYVGDTVGDMRMAVTARMEPVAVTYGNGTFGELAAVAG
                     TRMCSSFKDVVSVIAAARPRPTAAVTVGAQQRRR"
     misc_feature    complement(2024..2038)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.04"
                     /tool="antismash"
     misc_feature    complement(2050..2064)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     misc_feature    complement(2259..2272)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.61"
                     /tool="antismash"
     gene            2313..2966
                     /locus_tag="GR130_RS30030"
                     /old_locus_tag="GR130_30030"
     CDS             2313..2966
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574419.1"
                     /locus_tag="GR130_RS30030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_30030"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_159507611.1"
                     /transl_table=11
                     /translation="MPTQHRAIQSRQALIRSAAETFLEKGVPAAGMVEISRRARLSKGA
                     LYFHFTSKDDLTLAVRDAALATLQEIEDGFLRSPKPLSFAVRDFAAELFGRVQSDAVLR
                     AGLRLRPETAPGLEIHPLEQRWYALFLDKAVTCGTGGPVGTADGPAGPRHSDAEPRRTA
                     QLLTSMVVGLLHLGAADRTWWDQEAVTGLWALLPGAPGRVAAGCVPAPAQAAAG"
     gene            complement(3018..4046)
                     /locus_tag="GR130_RS30035"
                     /old_locus_tag="GR130_30035"
     CDS             complement(3018..4046)
                     /NRPS_PKS="Domain: PKS_ER (38-340). E-value: 2.9e-20.
                     Score: 64.6. Matches aSDomain:
                     nrpspksdomains_GR130_RS30035_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574420.1"
                     /locus_tag="GR130_RS30035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_30035"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_159507612.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.5e-18, bitscore:
                     65.9, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVPVPSDVPATHREVRLAGHPGNELTERHFTLAEAPVPQPVPGQ
                     VLVRTRVMAVTAAMRTQMTGARLPMPSFVPGEALWGSAVGEVVAAPGGGFTPGELVHHP
                     YGWREFAVVEEGRLRRLAPDALPSLAAHLSQGATAWGALRRGAGVRPGDTVFVSGAAGG
                     VGSLAGQLARRLGARRVVGSTGSERKASVLRAELGYDDTIVRGAGPIEEQLRRAAPDGI
                     DVMLDTVGGEQLAAALAVARPDARFVLVGALSSQLGEGGGVAAPVELDAGLIITRRVAL
                     RGFGLYAHPDLPEEWTREFGQGLRDGSLVFPHTLLKGIEQAPRALCELVQGRHIGAVLV
                     EL"
     aSDomain        complement(3027..3932)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR130_RS30035_PKS_ER.1"
                     /evalue="2.90E-20"
                     /label="GR130_RS30035_PKS_ER.1"
                     /locus_tag="GR130_RS30035"
                     /protein_end="340"
                     /protein_start="38"
                     /score="64.6"
                     /tool="antismash"
                     /translation="PQPVPGQVLVRTRVMAVTAAMRTQMTGARLPMPSFVPGEALWGSA
                     VGEVVAAPGGGFTPGELVHHPYGWREFAVVEEGRLRRLAPDALPSLAAHLSQGATAWGA
                     LRRGAGVRPGDTVFVSGAAGGVGSLAGQLARRLGARRVVGSTGSERKASVLRAELGYDD
                     TIVRGAGPIEEQLRRAAPDGIDVMLDTVGGEQLAAALAVARPDARFVLVGALSSQLGEG
                     GGVAAPVELDAGLIITRRVALRGFGLYAHPDLPEEWTREFGQGLRDGSLVFPHTLLKGI
                     EQAPRALCELVQGRHIGAVLV"
     CDS_motif       complement(3531..3593)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GR130_RS30035_0001"
                     /evalue="1.00E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="GR130_RS30035"
                     /protein_end="172"
                     /protein_start="151"
                     /score="11.8"
                     /tool="antismash"
                     /translation="GDTVFVSGAAGGVGSLAGQLA"
     gene            4240..4857
                     /locus_tag="GR130_RS30040"
                     /old_locus_tag="GR130_30040"
     CDS             4240..4857
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602525.1"
                     /locus_tag="GR130_RS30040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_30040"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_159507613.1"
                     /transl_table=11
                     /translation="MVKQARAVRTRRAVLEAAAHVIGTRGYQAATMAEIIQRAGVTKGA
                     VYFHFRSKDALARAVIREQTEPFVPQVSESRLQDAIDFTHQVALALRNDPLLQAGTRIA
                     VETTFSEDPLVPYQAWTDIITTMFSDARDNGELLPAVAPERAAEFFVSAYMGVQLFSRA
                     ATNRADLPERVTTLWKHTLPGLASPGALSHLDPQGRSVKIAV"
     misc_feature    4648..4650
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..5981
                     /locus_tag="GR130_RS30045"
                     /old_locus_tag="GR130_30045"
     CDS             5001..5981
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088827.1"
                     /locus_tag="GR130_RS30045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_30045"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_159507614.1"
                     /sec_met_domain="AfsA (E-value: 2.7e-24, bitscore: 83.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASATLTSFAPDQLRVGTHGRFADGNRPRSLTMTVPREYVHRTAV
                     SEVFLTDWRRGGPDSWLVSAQWPRAHSFYRPVNGLHDPLLLTETVRQAGILLSHVAHGV
                     PLDHPIIWRRVRYELSPTALRATDAPADVELRITDRDVVRHGEQLARVRQEFRIVCDGS
                     DLASAMLDYSCRSPAVYRRLRGDYGDLALANSRKLPVPEPVAPDLVGRDRTGDVVLSPA
                     DGPGRWQLRVDTSHPVLFDHPVDHAPGMLMIEAVRQATLALTPRGALPVTLECSFERYA
                     EMDAPCWVIARTAGRSGGGCREVEVGMEQHGRRVLAAKVGSLPMP"
     misc_feature    5286..5288
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6048..8387)
                     /locus_tag="GR130_RS30050"
                     /old_locus_tag="GR130_30050"
     CDS             complement(6048..8387)
                     /GO_function="GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537000.1"
                     /locus_tag="GR130_RS30050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_30050"
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="WP_236574039.1"
                     /transl_table=11
                     /translation="MTMSAARAGRPLKVAVLGGGPGGLYAAALLKRLDPAREITVWERN
                     APDDTFGFGVVLSDETLGGIEHADPVVYRALQAEFVRWDDIDIVHHGRTLTSGGHGFAA
                     LGRRRLLAVLQERCRSLGVRTVFRTEAPPAAELARDHDLVIAADGVHSTTRAAHADVFR
                     PQLTAHRCRYIWLSADFALDAFRFEIAETGHGIAQLHGYPYARPRPGAGPVEPAGGAST
                     VIVEMREEVWRAAGLDAYEERASAEWCAALFAGALGGRPLRSNNSSWTAFRTVVNERWS
                     HGRIVLLGDAAHTAHFSIGSGTKLAVEDALALAACLQEHATVPDALTAYEAERRPVVAS
                     TQRAARASLAWFEDLATYTGQPPHQFAFNLLTRSRRVTHGNLRLRDREFVDAVTKEAGI
                     PTGTPPMFTPFRLRGLTLRNRVVVSPMDMYSADGEGTPGDFHLVHLGARALGGAGLVMT
                     EMVCVSPEGRITPACTGLYTRAHETAWRRITDFVHTSAPGTAIGVQLGHSGRKGSTRRM
                     WDGIDQPLPDGNWPLVAPSALPYRPGVNQIPHALTAAELTAVRDRFARSAAAAARAGFD
                     LLELHCAHGYLLSGFLSPLTNQRTDAYGGSLAGRLRFPLEVFDAVREQWPDDRPMTVRI
                     SATDWADGGTTAEDAVAIAGAFAEHGADAIDVSTGQVVPDERPDFGRSYQTPFADRIRN
                     SLGIPVIAVGAISSWDDVNSLVLAGRADLCALARPHLYDPHWTLHAAADQGYTGPGAPW
                     PPPYQAGSRTPPTGRTDAPKPRLTLH"
     gene            complement(8384..9211)
                     /locus_tag="GR130_RS30055"
                     /old_locus_tag="GR130_30055"
     CDS             complement(8384..9211)
                     /NRPS_PKS="Domain: ECH (18-267). E-value: 4.6e-51. Score:
                     165.4. Matches aSDomain:
                     nrpspksdomains_GR130_RS30055_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982203.1"
                     /locus_tag="GR130_RS30055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_30055"
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="WP_159507615.1"
                     /transl_table=11
                     /translation="MSPFAGSARSTGAWQHIRVTREDGIATVTLARPEKLNALTFEAYA
                     DLRDLLAELSRERSVRALVLAGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVV
                     RAVRECPFPVIAAVHGVAAGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYL
                     LPRVIGLGHATRVLMLGDAIRAPEAERLGLISELAEEGRADETAAALARRLAEGPALAH
                     AQTKALLTAELDMPLAAAVEMDAATQALLMNSADYAEFHAAFTEKRTPKWQGK"
     aSDomain        complement(8411..9157)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR130_RS30055_ECH.1"
                     /evalue="4.60E-51"
                     /label="GR130_RS30055_ECH.1"
                     /locus_tag="GR130_RS30055"
                     /protein_end="267"
                     /protein_start="18"
                     /score="165.4"
                     /tool="antismash"
                     /translation="VTREDGIATVTLARPEKLNALTFEAYADLRDLLAELSRERSVRAL
                     VLAGEGRGFCSGGDVEEIIGATLGMDTAQLLDFNRMTGQVVRAVRECPFPVIAAVHGVA
                     AGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYLLPRVIGLGHATRVLMLGD
                     AIRAPEAERLGLISELAEEGRADETAAALARRLAEGPALAHAQTKALLTAELDMPLAAA
                     VEMDAATQALLMNSADYAEFHAAFTEK"
     misc_feature    9307..9322
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.28"
                     /tool="antismash"
     gene            9523..10056
                     /locus_tag="GR130_RS30060"
                     /old_locus_tag="GR130_30060"
     CDS             9523..10056
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014048884.1"
                     /locus_tag="GR130_RS30060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_30060"
                     /product="ATP-binding protein"
                     /protein_id="WP_236573660.1"
                     /transl_table=11
                     /translation="MPEPLLPEAGAPPLSEPSDVASWRVTLPHTPAAVPIARALITTAL
                     LDLRVTADHDTAALLTAELVANAVAHTGGSAPIELVVKLRPAGCEVEVHDGDPAPLAGL
                     ERPEDTAYGAPAAGPVPETGSAGAAAGRRPAARRGLRLVRGLSSAAGCRPTAHGKAVWF
                     TLPPLREPRLPRRP"
     misc_feature    complement(10141..10155)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.01"
                     /tool="antismash"
     misc_feature    complement(10366..10383)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 18.77"
                     /tool="antismash"
     misc_feature    10714..10729
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.22"
                     /tool="antismash"
     misc_feature    10866..10881
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.64"
                     /tool="antismash"
ORIGIN
        1 gccctacaaa tgcccgcggg tcttcgtctt ccacacggcg ctgccgcgca cccccaccgg
       61 caagctccag cgcttccggc tgcgcgaagc cgatctagag tgacccggtg agcgaccagc
      121 aagcccagca ggcccagcac accccacggt ccctgatcgt cacgttctac ggcgcctacg
      181 ggcgcggcgg cccggaccgt cccggcggcg cgcccgggcc ggtgccggtg gcggcgctga
      241 tccggctcct cggggcgctc ggggtggacc cgccgtcggt gcgctccgcc gtgtcccggc
      301 tcaagcgccg ggggctgctc gtcgcggagc gcaccgccgc cggcgccgcc gcctacggac
      361 tctccgacgc cgcccgccag ctcctggagg acggcgaccg gcggatattc ggccggccgg
      421 ccgcccgatt gtccgacggc tgggtgctgg ccgtgttctc cgtccccgag gaggagcggc
      481 acaagcggca cctgctgcgc tcccggctgt cccggctggg cttcggcacc gcggcgcccg
      541 gcgtgtggat cgccccggcg catctgtacg aggagacccg gcacaccctg gagcggctgg
      601 aactcggccc gtacgtcgac ctgttcaccg gtacccacgc cggtttcgcg cccaccgcgc
      661 aggccgtcgc gcgctggtgg gacctggacg ccatcgccgc acggcaccgc gcgttcctcg
      721 cggagcacga gccggtgctg gcgcgctggg cgcggcgccg gtcggttccc cccgaagcgg
      781 cctaccgcga ctacctgctg gcgctggacg cctggcgccg cctcccgtac gccgaccccg
      841 gtctgccctc tccgctcctc ccggaggact ggccgggcgg ccgggcggcc gaggtgttcg
      901 gccggctgga cgccaagttg cgggcggcgg gcgcccgtta cgcgtgtgag gcgacggccg
      961 ggccccaccg cacccgggga accgtctgac cggcccgccc tcgtacgccc gtacgccgtc
     1021 gcctctcctg cctgtggccg gtgtccccca ggccgcgcgc ccgccccccg tcccccgtcc
     1081 ccgtcgtgtc agcgtcgccg ctgctgcgcc cctaccgtga cggcggccgt cggccgcggc
     1141 cgggcggcgg cgatcaccga gaccacgtcc ttgaaggaac tgcacatgcg cgtcccggcg
     1201 accgcggcga gttcaccgaa cgtcccgttg ccgtaggtca ccgccaccgg ctccatgcgc
     1261 gcggtgaccg ccatccgcat gtcccccacg gtgtccccga catacgcgct ctgccaggcc
     1321 gggaccccca gccggccggc cgcgcgcagc accatctccg ggtgcggctt gccgcgccgg
     1381 accatgtcct gcccgatgac cgggccgacc aggtcacgga tgcccatgac gtccaacagg
     1441 gcctcggcgc cgcgggtcgt cttggacgtg gcgacggcga gttcgacgcc gtgggcgcgc
     1501 agcgcggaca gcccggccgt cactccgggg aagagcagct gcggcccctg gcacaggacc
     1561 tcgtacgaga acagctcgcg gtagcgggag atggcggcct gcacccggcc gtcctcctcg
     1621 ggccggccca gcagccggcc gaaggcggcg cccggaggct tgccgatggc ggccgccgcc
     1681 tgccgggcgg tgaccgaaca gccctgttcc gaagccacct tgacgagcag cttgctgatc
     1741 gcgggaaggg tgttggcgag ggtgccgtcg aggtcgaaga cggcggccct gatcgtctcg
     1801 gtgccggcgc tccgcgcgcc tcttacagga cgttcgccga cttccattga tacgcgcatg
     1861 gggtggagac ctttccgggt tccgatgggt gcgggggctc ggggtgacgt tgctggcaca
     1921 gctggtcggg cggccagcgg tcgacgaccg gtgcaggcag aggcggtctc gccacggcgc
     1981 accgctgtcc aagggcggta cgcgaccgtc ggggcggcgg catcggaagg gatcgtcccg
     2041 tttaccgcct tcggagattt aaaaaccgcc tgcgctgttt gtcaatgcgc ctttgtcaca
     2101 ccctgacggg tcgacaacac cccccggccg ggaccagact ggttacccgc gggtctcccc
     2161 ccgccgccgg ggtcggatca ccgggcgacc ggcggcttcg cgacagcgtc gggcagttcc
     2221 ggccaattac cccgagcata ccgaaccgca tggttggttt tctatgtaga ctgcaaggag
     2281 gttgcagtcg tacgagagac gggacggaca ccgtgcccac gcagcatcgc gccatccaga
     2341 gtcgccaggc gctcatccgc tcggccgcag agaccttcct ggaaaaggga gtgcccgccg
     2401 cgggcatggt cgagatcagc cggcgggccc ggctgagcaa gggagcgctc tacttccact
     2461 tcacgtccaa agacgacctg accctcgcgg tgcgggacgc cgcgctggcc accctgcagg
     2521 agatcgagga cggcttcctc cgctccccga agccgctctc cttcgccgta cgggacttcg
     2581 ccgccgagct gttcggccgg gtgcagtcgg acgcggtgct gcgcgccgga ctgcggctgc
     2641 ggccggagac cgcgccgggc ctggagatcc accccctgga gcagcgctgg tacgcgctct
     2701 tcctcgacaa ggcggtgacc tgcggcaccg gcgggccggt cggcaccgcg gacggcccgg
     2761 ccggcccgcg ccactcggac gccgaaccgc ggcgcaccgc ccagctgctg acgtcgatgg
     2821 tggtgggact gctgcacctg ggcgccgcgg accggacatg gtgggaccag gaggccgtca
     2881 ccggcctgtg ggcgctgctg ccgggcgcac cgggccgggt cgcggccggc tgcgtcccgg
     2941 cgccggcgca ggccgcggcc ggctgacccg cccggcccgc cgcgaccgga cgggcccgcg
     3001 ccgagcccgc gccgggctca caactcgacg agtacggccc cgatatggcg cccctgcacg
     3061 agttcgcaca gcgcccgcgg cgcctgctcg atccccttca gcagggtgtg cgggaagacg
     3121 agggagccgt cccgcaggcc ctggccgaac tccctggtcc actcctcggg caggtcggga
     3181 tgcgcgtaca gcccgaagcc gcgcagcgcg acccggcggg tgatgatcag ccccgcgtcc
     3241 agttcgaccg gagcggccac gccgccgccc tcgcccagct ggctggagag cgcaccgacg
     3301 agcacgaacc gggcgtccgg gcgggccacc gcgagcgccg cggcgagctg ctcgccgccc
     3361 acggtgtcca gcatgacgtc gatcccgtcc ggggccgccc ggcgcaactg ctcctcgatg
     3421 ggacccgcgc cgcgcacgat cgtgtcgtcg taccccagct cggcccgcag cacggacgcc
     3481 ttgcgctcgg agccggtgct gcccaccacc cgccgcgccc ccagccgccg cgccagctgc
     3541 ccggccaggc tgcccacgcc gccggcggcg ccgctgacga acacggtgtc gcccggccgc
     3601 accccggctc cccggcgcag cgcgccccag gcggtcgccc cctgggagag gtgcgcggcc
     3661 aaagagggca gcgcgtccgg ggcgaggcgc cttagcctgc cttcctccac caccgcaaac
     3721 tcccgccatc cgtaagggtg atggaccagt tcgccggggg tgaagccgcc gccgggggcc
     3781 gccacgacct cgccgaccgc ggagccccac agcgcctcgc ccgggacgaa cgacggcatc
     3841 ggcagccgcg caccggtcat ctgcgtccgc atcgccgcgg tcaccgccat cacccgggtg
     3901 cgtaccagca cctggccagg taccggctgc ggcaccgggg cttcggccag cgtgaaatga
     3961 cgctccgtca gttcattgcc ggggtggccg gcgagccgca cctcccggtg cgtggcgggc
     4021 acatcggacg ggacggggac gggcatcggg cagctccttg aacgcgcgcg gtgccgcgtg
     4081 acaccgctcc gcccgggtgc gacccgctgc cgcggcggcg ggtcggcgtg tgaataccgc
     4141 atggttggct tctttatgat gcggaaatag actgcgccgc cacggcagcg ggtcgcactc
     4201 aggcacaaca actccaagaa agcatacgga ggatgatcgg tggtcaagca ggcacgcgca
     4261 gtgcgcaccc ggcgcgccgt cctcgaagcc gcggcccatg tcatcggcac tcgtggatac
     4321 caggccgcca cgatggccga gatcatccag cgcgccgggg tcaccaaggg cgcggtctac
     4381 ttccacttcc gctcgaagga tgcactcgcc cgcgcggtca tccgggagca gaccgagccg
     4441 ttcgtgccgc aggtgagcga gtcccggctg caggacgcca tcgacttcac gcatcaggtg
     4501 gcgctggcgc tgcgcaacga cccgttgctg caggccggga cccggatcgc ggtcgagacg
     4561 accttcagcg aggatccgct ggtcccctac caggcgtgga ccgacatcat caccacgatg
     4621 ttcagcgacg cccgggacaa cggggagtta ctgcccgccg tggcgccgga gcgagccgcc
     4681 gagttcttcg tctccgccta tatgggcgtg cagttgttct cgcgggccgc caccaatcgc
     4741 gccgaccttc cggagcgggt cacgaccctg tggaagcaca ccctgccggg tctcgcctcg
     4801 ccgggggcgc tgagtcatct ggacccgcag ggccgctccg tgaagatcgc cgtctgaccg
     4861 accgccgctc gtcgccctga cggggtaatg gtccactcgg cactggaaaa caaaccacat
     4921 ggttcgtatc ttggtgcccc gggccggaga ccggactcgg tccgtcaggg cgctgagcgc
     4981 cgcccacaga aaggggcccc atggccagcg ccaccctcac ctcgttcgcg cccgatcagc
     5041 tccgcgtcgg cacccacgga cggttcgccg acggaaaccg accgcggtcc ctgaccatga
     5101 ccgtgccccg cgagtacgtg catcgcaccg ccgtctccga ggtgttcctg acggactggc
     5161 ggcgcggcgg ccccgacagc tggctggtga gcgcccagtg gccgcgggcg cacagcttct
     5221 accggccggt gaacggcctg cacgacccgc tgctgctgac cgagaccgtg cggcaggcgg
     5281 ggatattact gagccacgtc gcgcacggcg tgccgctgga ccacccgatc atctggcgca
     5341 gggtccgcta cgagctctcg ccgacggcgc tgcgcgccac ggacgcgccg gccgacgtgg
     5401 agctgcgcat caccgaccgc gacgtggtgc ggcacggaga gcagctggcc cgcgtccgcc
     5461 aggagttccg catcgtgtgc gacggcagcg acctggcctc cgcgatgctc gactactcct
     5521 gccgcagccc ggccgtctac cgccggctgc gcggcgacta cggcgatctt gcgctggcca
     5581 actcccggaa gctgccggtg ccggagccgg tcgccccgga tctggtgggc cgggaccgca
     5641 ccggtgatgt ggtgctctcc cccgccgacg gcccgggccg ctggcagctg cgggtggaca
     5701 cctctcatcc ggtgctgttc gaccatcccg tggaccacgc gcccggcatg ctgatgatcg
     5761 aggccgtccg gcaggccacg ctggcgctga cgccccgtgg ggcgctgccg gtcacgctgg
     5821 agtgctcctt cgagcggtac gcggagatgg acgcgccctg ctgggtgatc gcccggaccg
     5881 ccgggcgctc cggcggcggc tgccgggagg tcgaggtcgg catggagcag cacggccggc
     5941 gggtgctcgc cgccaaggtc ggcagcctgc cgatgccctg agagcctccc tcccggcgcc
     6001 gcaccgcccc ccgtccgccc cgcctcccgc tgcttgtggc ctccccgtca gtgcagcgtg
     6061 aggcggggct tcggcgcgtc ggtgcggccg gtgggcggag tgcggctgcc ggcctggtag
     6121 ggcggcggcc agggggcgcc cggccccgtg tagccctggt cggcggcggc gtgcagggtc
     6181 cagtgcgggt cgtagaggtg cgggcgggcc agggcgcaca ggtccgcgcg cccggccagc
     6241 accagggagt tgacgtcgtc ccaggaggag atcgcgccga ccgcgatgac ggggatgccg
     6301 aggctgttgc ggatccggtc ggcgaacggc gtctggtacg agcggccgaa gtcggggcgc
     6361 tcgtcgggta cgacctgtcc ggtggagacg tcgatggcgt cggcgccgtg ctcggcgaag
     6421 gcgcccgcga tggccaccgc gtcctcggcg gtggtgccgc cgtcggccca gtcggtggcg
     6481 gagatgcgga ccgtcatggg ccggtcgtcc ggccactgtt cgcggaccgc gtcgaagact
     6541 tcgaggggga agcggagccg gcccgccagg gaacctccgt acgcgtcggt gcgttggttg
     6601 gtcagcggag agaggaaccc ggagaggaga tagccgtggg cgcagtggag ttcgaggagg
     6661 tcgaatccgg cgcgcgcggc agcggcggcg gagcgggcga accggtcgcg tacggcggtg
     6721 agttcggcgg cggtcagggc gtgcgggatc tggttgacgc cggggcggta gggcagtgcg
     6781 gagggggcga cgagcggcca gttgccgtcg gggagcggct ggtcgatgcc gtcccacatc
     6841 cgccgggtcg agcccttgcg gccggagtgg ccgagctgca cgccgatggc ggtgccgggg
     6901 gccgaggtgt ggacgaagtc ggtgatccgg cgccaggccg tctcgtgggc gcgggtgtag
     6961 agcccggtgc aggccggggt gatccgccct tcggggctga cgcacaccat ttccgtcatg
     7021 accaggccgg cgccgccgag cgcgcgggcg ccgaggtgga cgaggtggaa gtcgccgggg
     7081 gtgccctcgc cgtcggcgga gtacatgtcc atcggggaga ccacgacccg gttgcgcagg
     7141 gtcagtccgc gcagccggaa gggggtgaac atgggcggtg tcccggtggg gatgcccgcc
     7201 tccttcgtca ccgcgtcgac gaactcccgg tcgcgcagcc gcagattgcc gtgggtgacc
     7261 cggcggctgc gggtgaggag gttgaaggcg aactggtgcg gcggctgccc cgtgtaggtg
     7321 gccaggtcct cgaaccacgc gaggctggcg cgggcggcgc gctgggtgga ggcgacgacg
     7381 gggcggcgct cggcctcgta ggcggtgagc gcgtccggca cggtggcgtg ctcctggagg
     7441 caggcggcga gggcgagggc gtcctcgacg gcgagtttgg tgccggagcc gatggagaag
     7501 tgcgcggtgt gcgcggcgtc gccgaggagg acgatccggc cgtgcgacca gcgctcgttg
     7561 acgacggtgc ggaaggcggt ccagctggag ttgttggacc gcagcgggcg gccgcccagc
     7621 gcgccggcga agagcgccgc gcaccattcg gccgaggcgc gctcctcata ggcgtccagg
     7681 ccggcggccc gccagacctc ctcgcgcatc tcgacgatga cggtgctggc gccgccggcg
     7741 ggctccacgg ggccggcgcc gggtcgcggc cgggcgtacg gatagccgtg cagctgggcg
     7801 atgccgtggc cggtttcggc gatctcgaag cggaaggcgt ccagcgcgaa gtcggcggac
     7861 agccagatgt agcggcagcg gtgggcggtc aactgcgggc ggaagacgtc ggcgtgggcg
     7921 gcgcgggtgg tgctgtgcac gccgtcggcg gcgatcacca ggtcgtggtc gcgggcgagt
     7981 tcggcggccg gcggtgcctc tgtgcggaag acggtgcgca caccgagcga gcggcagcgt
     8041 tcctggagga cggcgagcag ccggcgccgg ccgagcgcgg cgaaaccgtg gccgcccgag
     8101 gtcagggtgc ggccgtggtg cactatgtcg atgtcgtccc agcggacgaa ctccgcttgc
     8161 agggcgcggt agacgaccgg gtcggcgtgc tcgatgccgc cgagggtctc gtcggagagg
     8221 acgacgccga agccgaaggt gtcgtcgggg gcgttgcgtt cccagacggt gatctcgcgg
     8281 gccgggtcga ggcgtttgag cagcgccgcc gcgtagagcc cgccggggcc gccgccgagg
     8341 acggcgacct tcagcggccg gccggcccgc gccgcggaca tcgtcatttc ccctgccact
     8401 tgggggtccg cttctcggtg aaggcggcgt ggaattcggc gtagtcggcg ctgttcatca
     8461 gcagtgcctg ggtggcggcg tccatttcga cggcggcggc cagcggcatg tcgagctcgg
     8521 cggtgagcag cgccttggtc tgggcgtggg ccagcgcggg cccttcggcg agccgccggg
     8581 cgagcgcggc ggccgtctcg tcggcgcggc cctcctcggc caactcgctg atcaggccga
     8641 ggcgttcggc ctcgggcgcc cggatcgcgt cgccgagcat cagcacccgg gtggcgtgtc
     8701 cgaggccgat gacgcggggc aggaggtagg ccgcgcccat gtcgccgccg gagagaccga
     8761 cccgggtgaa gaggaacgcg aagcgggcgg aggggtcggc gacgcggaag tcggcggcca
     8821 gcgcgagcac cgcgccggcg ccggccgcca cgccgtgcac cgccgcgatg accgggaagg
     8881 ggcactcgcg caccgcccgt acgacctggc cggtcatccg gttgaagtcc agcagctgtg
     8941 cggtgtccat gcccagggtg gcgccgatga tctcctcgac gtcgccgccg gagcagaagc
     9001 cgcggccttc cccggcgagg accagggcgc gcacggagcg ttctctggac agttccgcga
     9061 gcaggtcgcg gaggtcggcg tatgcctcga aggtgagcgc gttgagcttc tcggggcgcg
     9121 cgagggtgac ggtcgcgatg ccgtcttccc tggtcacccg gatgtgctgc caggcgccgg
     9181 tgcttcgggc ggatcctgcg aacgggctca tcggtctgcc tgcccccctc agccggacgg
     9241 ctggtgtgtg cggtggctgc ggatgcggtg gcggtgcgcg tgaacccctc gaagctatca
     9301 ctcatttgtg actgccgtca tgatggcgcg atacatcgtc ccgtgtgcgc cgctcgctgc
     9361 gggcgggtgc gtaggggccg aacgtcccat ccggcgggtg acccaggtcc cggtcggccc
     9421 tgccgttcgg ctctgatgcc cggcccggcc ccggccctac ggtgaaggtg tccgatgggg
     9481 ccatgaggga tccgacccgg ccccgggcga acggaacacc tgatgccgga accactcctg
     9541 ccggaagccg gcgccccgcc gctgtccgaa ccgtccgacg tcgcctcctg gcgcgtcacg
     9601 ctgccgcaca ccccggccgc cgtgccgatc gcccgcgcgc tgatcaccac cgcgctgctg
     9661 gacctgcggg tcaccgccga ccacgacacc gcggcgctgc tgaccgcgga gctggtcgcc
     9721 aacgccgtgg cgcacaccgg cggttcggct ccgatcgagc tggtggtgaa gctgcggccg
     9781 gcgggctgcg aggtcgaggt gcacgacggg gacccggcgc ccctggcggg gctggagcgg
     9841 ccggaggaca cggcgtacgg cgcaccggcg gcggggccgg ttccggagac cgggagtgcc
     9901 ggcgcggcgg ccggccggcg cccggccgcg cgccgcgggc tgcggctggt ccgggggctc
     9961 agctccgccg ccggctgccg gccgaccgcg cacggcaagg ccgtgtggtt caccctgccc
    10021 ccgctgcggg agccgcggct gccgcggcgg ccctgacccg ctgcccgccg gtaccgcgtc
    10081 ggcccggccg gtgccgggcc cgcgctgcgg gggaaccgcc gagcggctgc cccggcttcc
    10141 cggaccggct actcctggac ggccccgacc cggtcccgga tcctgccctc ggtcccgccg
    10201 gcgcccggct ccccgtccgc tcccccgacg gtgtccccgc ccggctcccg ggtcccgctc
    10261 ccgaggcggg agtggcggcg cccgtacagc gcgtagacca gcgcgccgac ggccaggaac
    10321 ccggcgaact gcagccaggt cgccgggccg gtgccgtaga tgagatagcc gcagaaggcg
    10381 atgccgagga ccgggctgac cgggaagagc gggacgcgga agcggcgcgg caggtcgggc
    10441 cgggtgcggc gcaggaccag cacgctgaca ttgaccacgg ccatgatcgc cagggtcccg
    10501 atggtggtca ggttcatcac cacgtcgagc gggacgaccg cggcggggac cgcgaagacg
    10561 gccgcgacga tccaggtgtt ggcgaccggg gtggaggtcc ggggcgagat ccgttcgaag
    10621 atccgcggga tcagcccgtc ccgggacatc gacatcagga tgcgcgtctg cccgtacatc
    10681 acggcgagga cgacggaggc gatggcgacc accgcgccga aggcgatcac gccgccgccg
    10741 agggtggagt gggtgacgtg gttgacgatc agcgacagtg cggccggctg cccggcgacc
    10801 ttcccggacg agagcgcgcc gatggcgccg agcgcgacca ggcagtacag gacggtgacc
    10861 acgccgatgc agatcatgat cgcgacgggg atgttgcgcc gcgggttgcg gacctcttca
    10921 ccggcggtcg tgacggcgtc gaagccgatg tacgagaaga acgcgacgga cgctccggag
    10981 g
//
