LOCUS       NZ_CP047146            11429 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. GS7 chromosome, complete genome.
ACCESSION   NZ_CP047146
VERSION     NZ_CP047146.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GS7
  ORGANISM  Streptomyces sp. GS7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9067183)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9067183)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-NOV-2019
            Assembly Method                   :: SPAdes v. Nov-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:53:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,019
            CDSs (total)                      :: 7,925
            Genes (coding)                    :: 7,616
            CDSs (with protein)               :: 7,616
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 107 of 309
            Pseudo Genes (incomplete)         :: 258 of 309
            Pseudo Genes (internal stop)      :: 29 of 309
            Pseudo Genes (multiple problems)  :: 79 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:40:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6847389
            Orig. end                         :: >6858818
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047146.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..>11429
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="30"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..>11429
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..>11429
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:>6429](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..>6429
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(596..1540)
                     /locus_tag="GR130_RS40040"
     CDS             complement(596..1540)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602442.1"
                     /locus_tag="GR130_RS40040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_201305233.1"
                     /transl_table=11
                     /translation="MRMDGVGRRYGMRGPWVLRDVQLDVPVGALLRIEGTNGSGKSTLL
                     RLLAGIDRPTAGRITGRPRTAYVPERFPAALPFTAAGYLTHLGRVHGLRTPQAARRAAE
                     WLERFGAAGHAHTPLAELSKGTSQKVAVAQALLAEPELLVLDEAWTGLDRAARGLLDRA
                     VAERVAGGGTVVFVDHDPRRLAGAADSVHRVADGLLLPQEDGAEERRAAPVGPRVVIEA
                     EGPPESVPGSAGPLPGAPDIVPGPGGTTRLTVPAAHSDALLRALLTAQPPWHIRAVTAA
                     APSQGTAHEPPHDTSRAAQQATLQDTPYEAPLS"
     gene            complement(2046..2216)
                     /locus_tag="GR130_RS29780"
                     /old_locus_tag="GR130_29780"
     CDS             complement(2046..2216)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018542789.1"
                     /locus_tag="GR130_RS29780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_29780"
                     /product="hypothetical protein"
                     /protein_id="WP_236573641.1"
                     /transl_table=11
                     /translation="MGEQLDLLRTALQDRTAWMDGLTEQDLEEMLRGLHVCIDAVQATG
                     SDAPERPAGTS"
     gene            complement(2473..2796)
                     /locus_tag="GR130_RS29785"
                     /old_locus_tag="GR130_29785"
     CDS             complement(2473..2796)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540923.1"
                     /locus_tag="GR130_RS29785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_29785"
                     /product="hypothetical protein"
                     /protein_id="WP_159507576.1"
                     /transl_table=11
                     /translation="MSNATARFLIALLRLLFPARGCQRSASTPPIASCEDDSTVALPRV
                     PARRPELTLLRGEDVAFLRPYVLTPKECQERRLQRGRRRALWLWTHGIDAGPRWIHGVE
                     VTG"
     misc_feature    complement(2770..2772)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(2811..2825)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.73"
                     /tool="antismash"
     misc_feature    complement(2883..2903)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.16"
                     /tool="antismash"
     gene            2997..3743
                     /locus_tag="GR130_RS29790"
                     /old_locus_tag="GR130_29790"
     CDS             2997..3743
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004937945.1"
                     /locus_tag="GR130_RS29790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_29790"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_236573642.1"
                     /transl_table=11
                     /translation="MTDANHPYMQVAERIRRRILDGALKEGEKLPPVRELARQEGVSVA
                     TLGRSLDQLQVEGYITTSRRGTFVANAPEVAPSGYDRITRVLRTGSVLADGETMIVTHA
                     ELIRPPQYISEIFDLSEGDQVVRRQWHTGKGQQRTGLFVTWYPAHFAAAVPELLSTAAK
                     SSAGLLPKIQKAIGRRVVAGRDDIHGRDADVREANYLGLRTGSPILAGAHRLWDNDGVI
                     EFGEWCLPYRLTVGYEYSFEAAPDGS"
     misc_feature    complement(3028..3040)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.34"
                     /tool="antismash"
     misc_feature    complement(3782..3795)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.37"
                     /tool="antismash"
     gene            4121..4822
                     /locus_tag="GR130_RS29795"
                     /old_locus_tag="GR130_29795"
     CDS             4121..4822
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536944.1"
                     /locus_tag="GR130_RS29795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_29795"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_159510303.1"
                     /transl_table=11
                     /translation="MRDEAGAEEAPQFVLPLVVRIERAAPPARTDALETAARAVLVLLS
                     DERAAGEGEWAQAVRDWEDSRIRKVVRRARGAEWRRAEALPGITVTGRDAEVRVFPPVP
                     LDGWPKDLARLQVSGTELDDPEPCGEPAAGEPVLWLNPGLEMSAGKAMAQAGHGAQVAW
                     WTLPEAARVAWREAGFPLAVRVAEPAAWEALTRSGLPVVRDAGYTEIAPGSCTVVADHP
                     ALQGSWPSPVR"
     gene            5001..>6429
                     /locus_tag="GR130_RS29800"
                     /old_locus_tag="GR130_29800"
                     /pseudo=""
     CDS             5001..>6429
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547187.1"
                     /locus_tag="GR130_RS29800"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="GR130_29800"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 2.3e-96, bitscore: 321.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIAAEIAALPGVDWVEVVAENVCPGHLPSALRE
                     LRERGTTVVPHGVSLGLGGADRPDAGRLAALAERATALGSPLVTEHIAFVRAGGPLTAS
                     PVVEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALVNWPGEEMTEGRFLS
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAATLDGLPVEAIAYVHVAGGVERDGVWHDSH
                     AHPVTQPVLDVLAELCARIAPPGVLLERDDDFPEGGELAGELDAIRAVVKGGVRHAQAA
                     "
     gene            6929..8632
                     /locus_tag="GR130_RS29805"
                     /old_locus_tag="GR130_29805"
     CDS             6929..8632
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (91-507). E-value:
                     4.7e-17. Score: 54.3. Matches aSDomain:
                     nrpspksdomains_GR130_RS29805_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570335.1"
                     /locus_tag="GR130_RS29805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_29805"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_236573644.1"
                     /sec_met_domain="PF00561 (E-value: 1.8e-21, bitscore: 75.8,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYSALGSLVLTPLATAPAGGAVRDPAAPPAPVHPISFGRC
                     AAVERLPAAVECGTLAVPLDYARPNGRKIRLTVSRARATAPGERQGALVFNPGGPGASS
                     MQFPLYGGLPTWRQTARSYDFVGYAPRGVGRSAPLSCQDPKEFTKAPTDSPKYPSDAFK
                     RQKVAEAKAYARGCAQRAGADLPYYNSLNNARDLDMLRAALGERKLTFMGASYGTYFGA
                     VYATLFPGHVRRMIFDSIVNPQPQQIWYRNNLDQSVAFESRWSDWLRWVAKHNDTYHLG
                     TSKAAVQRAYDTAVEQLRRKPVNGKIGPAQLQAAFLKTGYNDLFWPMRAHALSKYLHGD
                     AKQLIAQAMPSGDGKDEENSNAVYTAVECNDAAWPRNWSVWDRDNTRIARTAPFETWDN
                     VWMNLPCAFWPDRARSAQAGVDDAVRGTADRAAQGTLGAGELADASLQAADDLDDTLHG
                     RRRPLDIRTEPGALPPVLLLAAERDAATPYPGALELQRRLPGSSLVTERKAGTHGIALG
                     GNACVNAYVEAYLVRGRVPGHRVYCGPRAEPVPGQQLQRARQLVQQAQGK"
     misc_feature    complement(6956..6975)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.45"
                     /tool="antismash"
     aSDomain        7202..8449
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR130_RS29805_Abhydrolase_1.1"
                     /evalue="4.70E-17"
                     /label="GR130_RS29805_Abhydrolase_1.1"
                     /locus_tag="GR130_RS29805"
                     /protein_end="507"
                     /protein_start="91"
                     /score="54.3"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMQFPLYGGLPTWRQTARSYDFVGYAPRGVGRS
                     APLSCQDPKEFTKAPTDSPKYPSDAFKRQKVAEAKAYARGCAQRAGADLPYYNSLNNAR
                     DLDMLRAALGERKLTFMGASYGTYFGAVYATLFPGHVRRMIFDSIVNPQPQQIWYRNNL
                     DQSVAFESRWSDWLRWVAKHNDTYHLGTSKAAVQRAYDTAVEQLRRKPVNGKIGPAQLQ
                     AAFLKTGYNDLFWPMRAHALSKYLHGDAKQLIAQAMPSGDGKDEENSNAVYTAVECNDA
                     AWPRNWSVWDRDNTRIARTAPFETWDNVWMNLPCAFWPDRARSAQAGVDDAVRGTADRA
                     AQGTLGAGELADASLQAADDLDDTLHGRRRPLDIRTEPGALPPVLLLAAERDAATPYPG
                     ALELQRRLPGSSLVTER"
     gene            complement(8731..9465)
                     /gene="hemQ"
                     /locus_tag="GR130_RS29810"
                     /old_locus_tag="GR130_29810"
     CDS             complement(8731..9465)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536947.1"
                     /locus_tag="GR130_RS29810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_29810"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_159507577.1"
                     /transl_table=11
                     /translation="MDHMTDASTAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPED
                     RGGYADEVEELFAQLAAKDVVVRGTYDVSGLRADADLMIWWHSESSDALQEAYNLFRRT
                     RLGRALEPVWSNMALHRPAEFNKSHIPAFLADEEPRAYVSVYPFVRSYEWYLLPDEDRR
                     RMLADHGKMARGFPDVRANTVPSFSLGDYEWVLAFEADELYRIVDLMRHLRGSEARMHV
                     REEIPFYTGRRKPVAELVAGLA"
     gene            complement(9468..10928)
                     /gene="hemG"
                     /locus_tag="GR130_RS29815"
                     /old_locus_tag="GR130_29815"
     CDS             complement(9468..10928)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088886.1"
                     /locus_tag="GR130_RS29815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_29815"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_159507578.1"
                     /sec_met_domain="DAO (E-value: 3.9e-11, bitscore: 42.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAAHTSRGRAPGHVVVIGGGIAGLTAAHHLLEGGARVTLLEASG
                     RLGGKLLAGEIAGVPVDLGAESMLARRPEAVELARAVGLGERLQPPGTATASLWTRGAL
                     RPMPKGHVMGVPGDLAPLAASGVISPEGMARIAEDSTLARTEAGTDVAVGEYVAARLGR
                     EVVDRLVEPLLGGVYAGDAYRISMRAAVPQLFEAARTERSLIEGVRAIQARAAALVPPG
                     GPVAGGSVFMGIDGGIGTLPGAVADAVRRAGGEILLGTEVRELRHTAADGWRIAAGDRR
                     IAADAVIMAAPAGAAARLLRAGCPAAADELETVDYASMALVTMAFRRSDLGTVPSGSGF
                     LVPPVDGHRIKASTFASHKWDWIARADPDLFVLRTSLGRYGDDADLKRDDAELVELSLT
                     DLGAAVGLAARPVATTVTRWDGGLPQYAVGHVDRVDRIRAAVAGAPGPLRVCGALYEGV
                     GIPACIGSARRAADEILGTLAAVPAEGE"
ORIGIN
        1 aacggcatcc agggggcggt gaccgtgccg tgctcggaac cggtgatcat gcccgtcagc
       61 gcggcgttgg cgggcgagcc gctcaggacg aggagcagca gggcggcgac cagcccgacg
      121 gggacgcccc agccggggcg gcggagcacg ggccagttgc acagcgcacc ggtcgcgacg
      181 cccggcagca ggcaggtcag cgcggtgagc agcccggcac cggtcgcggg caggaccgga
      241 acggcgacct ggtgatcgct ggagcgcggg tcggacgaca gggcgacaaa cgccgtaccg
      301 aggagcgcga ggagcagcgc ggcaccgatc gcggtcagta cgcaggcgag gtgcacccgg
      361 ctgggcgtgg tggccgccgc ggcgcaattg cgggcggccg gcggctcgtt ggtgacgcag
      421 acccgggtga gccagacggc gaccggcagc agaccggcgg cggcatagcc gtacgcgtcc
      481 aggatcgggc cgccggtctg gaccccgatg gccaggaacg ccgcgtaggc gatgaccggc
      541 ggcagccagc ggtgcgagcg gacgagcagg gccgcctgat agcggagcag tgcggtcatg
      601 agaggggggc ctcgtaaggg gtgtcctgga gagtggcctg ctgggcggcc cgggaggtgt
      661 cgtgcggggg ttcgtgggcg gtgccctggg atggcgcggc ggcggtgacc gcgcggatgt
      721 gccagggggg ttgggcggtg agcagggcgc gcagcagcgc gtcggagtgg gcggccggga
      781 ccgtcagccg ggtcgtaccg ccggggccgg ggacgatgtc cggcgccccg ggcaggggcc
      841 cggcgctccc cgggacgctt tccggcggcc cctccgcctc gatgacgacc cgcgggccca
      901 ccggggccgc ccgccgctcc tccgcaccgt cttcctgggg cagcaacagc ccgtccgcga
      961 cgcggtgtac ggaatccgcg gcgcccgcca gccggcgcgg atcgtgatcg acgaagacga
     1021 cggtgccgcc gccggcgacc cgctcggcga ccgcgcggtc gagaagcccg cgcgcggccc
     1081 ggtccaggcc ggtccacgcc tcgtccagga cgaggagttc gggctcggcg agcagcgcct
     1141 gggcgaccgc gaccttctgg ctcgtcccct tggacagctc ggccagcggc gtgtgcgcgt
     1201 gcccggccgc accgaaccgt tcgagccact cggcggcacg gcgggcggcc tgcggggtgc
     1261 gcaggccgtg aacgcggccg aggtgggtga ggtagccggc ggccgtgaac ggcagcgccg
     1321 ccgggaaccg ttcgggtacg taggcggtac gcgggcgtcc ggtgatccgt ccggcggtgg
     1381 gccggtcgat accggcgagc agccgcagca gcgtggactt accgctcccg ttggtcccct
     1441 cgatacgcag caacgcacct acgggaacgt cgagttgcac atcgcgcaac acccatggtc
     1501 cccgcatccc gtaacgccgc ccgaccccgt ccatcctcat ccgctgcgac aacgcttccc
     1561 ctcccccgtc cttatggcca cggcgaccca tgaggccgtg gaccgctgcg acgcccgctg
     1621 gcctgtggtg gctcgtgccg gccggtagca cgctcccggc acgacggtct cgccggcggg
     1681 cgctccggcc tccttacgcc gtcgacggta gccggaaccg tcaaccggcg ccttgccggg
     1741 ggcggtcgcg gccgtgcggg gtgggcgggg ctgggttgtg ggtgtcagtc ccgccctggt
     1801 ccaagtcggc ggagtgccgt tcaccgacgg gcagccgctg aagacagccc tgcagtagtc
     1861 gaacgccgtc aactcgaaga ggtcgtcacc agtggcacgt gcgtgcccaa gacaccactc
     1921 ccgagcttcc tccggcgttg cggtatcgac gcagaactcc ccgcagattt ggcacttccc
     1981 cacgtgcctc tgctgccacc ccggatggac caagagcgtc cattcccgcg acctcttacg
     2041 cgccctcatg acgtgcccgc cggacgctcc ggggcatcac tgcctgttgc ctgtacggca
     2101 tcgatgcaca cgtgcagacc acgcagcatc tcttcgagat cctgctccgt gaggccatcc
     2161 atccacgcgg tacggtcctg gagtgccgtc cgcagcagat caagctgctc gcccaactcc
     2221 ccttgccttg caggctggtt ccacgctcgc tctccccggt cgcacgaccg ccaccaggcg
     2281 ctcatttgcg gcccgccttc gggtgggggt gctgcccgat ctcgaccgtg cacatagcgg
     2341 ggcgaccgtg ctcctggcgc ttccgagctt cggctcgctg cttcaccagc tcccggcaga
     2401 cgtcgcaccc cggcgccggt tcagggttcc cgtacgggtc cggcaggtgc acggggccgc
     2461 tcagactggg catcagccgg tcacctccac accgtgaatc caacgcggcc cggcatcgat
     2521 gccgtgcgtc cagagccaca acgcccgcct ccggccacgc tgcaagcgcc gctcctggca
     2581 ctccttgggg gtgaggacgt acggacggag gaacgcgacg tcctcaccac gcagcagcgt
     2641 caactccggt cggcgagcgg ggactcgggg gagtgcgacc gtcgagtcgt cctcacacga
     2701 cgcgatgggc ggcgtgctgg ccgaccgttg gcagccacgg gccgggaaga gcaggcgcag
     2761 cagcgcgatt aagaacctcg ctgtagcgtt ggacatgctg tcaactcctt tgaagttgaa
     2821 ggccatgccc ccggacggat tgcccccgtc gcgggggttc agacatccgt gtcctaggtc
     2881 agaacatagc gtgacctagg acagcacaac aggggatcgg ccgatctcgc ccgcacggat
     2941 tacgcgtgtc ctagtacatt gatcagtgac ctaggatgcc gacgacagga ggcgctatga
     3001 cggacgccaa tcacccgtac atgcaggttg cggaacgcat ccgacgccgc atcctggatg
     3061 gcgccctgaa ggagggtgaa aagctcccgc ccgtgcggga gctggcccga caagaagggg
     3121 tgtcggtcgc caccctcggt cggtccctcg atcaactgca ggtggagggg tacatcacca
     3181 catcacgcag ggggacgttc gttgccaacg ccccggaggt tgcgccttcg ggctacgacc
     3241 gaatcactcg cgtcctgcgt actggttcag tcctggccga tggggagacg atgatcgtca
     3301 cccacgccga gctgatcagg ccaccgcagt acatctccga gattttcgat ctttctgagg
     3361 gtgaccaggt ggtgcgccgt cagtggcaca ccggcaaggg gcagcagcgg accggcctat
     3421 tcgtgacgtg gtaccccgcc cacttcgccg cagccgttcc cgagctgctc tctaccgccg
     3481 cgaaatcctc ggccgggcta ctgccgaaga tccagaaggc cattgggcgc cgcgtcgtcg
     3541 cgggtcgcga cgacatccac ggcagggacg ctgacgtacg ggaagcgaac taccttggtc
     3601 tccgtaccgg ttcacccatc ctggctggtg cgcatcggct ctgggacaac gatggtgtca
     3661 tcgaattcgg tgaatggtgc ctgccctacc ggctgaccgt cggctatgaa tacagcttcg
     3721 aggctgcgcc cgacggctcc tgagccaagt tcgccagacc aagtacggcc acccaccgtc
     3781 atcacggagg gtggccgttt gcacgcccgg acccgagctt caaccatgtc tcagaatctg
     3841 aggtctctcg ctactccccc ctcccctcac tccccgcgtt gtcagtgccc gctggcacac
     3901 tgagcgagtg accagtaccg agaccgccgg gcaggccggc gacagcgcca gcagcccgtc
     3961 tgggcgtgct gaggcccacc acgccgagcc gggccacacc ccgaccgatc gccccgtggc
     4021 cgaccgcccc gcgacccacc gcatcgagac cacccacccc gacgccggtc gccacgaggc
     4081 cgctcgccgt gaggctcggc ccggcgagga cgaccggagc gtgcgggacg aggcgggcgc
     4141 ggaggaggca ccgcagttcg tgctgccgct cgtcgtgcga atagagcggg ccgcaccgcc
     4201 tgcgcgtacg gacgcgctgg agacggcggc gcgggcggtg ctggtgctgc tgtccgacga
     4261 gcgggccgcc ggggagggcg agtgggccca ggccgtgcgg gactgggagg actcccggat
     4321 ccggaaggtc gtacggcggg cccgaggcgc ggagtggcgg cgtgccgagg cgctgccggg
     4381 catcaccgtc accggccgcg atgccgaggt gcgggtcttc ccgccggtgc cgctggacgg
     4441 ctggccgaag gatctggcgc ggctccaggt ttcggggacc gagctcgacg atccggagcc
     4501 gtgcggggag ccggccgccg gggagccggt gctgtggctc aatcccgggc tggagatgtc
     4561 ggcgggcaag gcaatggccc aggcggggca cggcgcccag gtggcgtggt ggacgctgcc
     4621 cgaggcggca cgtgtcgcct ggcgggaggc gggattcccg ctggccgtac gggtcgcgga
     4681 gcccgcggcg tgggaggcgc tcacccggag cgggctgccc gtggtgcggg atgccgggta
     4741 tacggagatc gcgccgggct cgtgcacggt cgtcgcggac catccggcac ttcaggggtc
     4801 ctggccgtcg ccggtgcgct gaccggtcga cgcccgtcga gcggtcaacc gacccacccg
     4861 gcaaccgccc cgccccgaag ctcaaatcaa gctctgaagc acacgggcgc atgattagcc
     4921 cgccttgacg aggtcatgag tactccgacc acggagctag acaagcacgg agcaggacaa
     4981 ggggggcaag agggggagca atggagcggc ttggtacggg catcggctgg cggccggaga
     5041 tcgccgcgga gatcgccgcg ctgcccggcg tggactgggt cgaggtggtg gccgagaacg
     5101 tctgccccgg acatctgccg tccgccttgc gggagttgcg cgagcgcggc accaccgtcg
     5161 taccccacgg cgtctcgctc ggcctcggcg gcgccgaccg tccggacgcc gggcggctgg
     5221 ccgcgctcgc cgaacgcgcc acggccctgg gttccccgct ggtcaccgag cacatcgcgt
     5281 tcgtccgtgc cggcggcccg ctgaccgcgt ccccggtcgt ggaggccggg catctgctgc
     5341 cggtgccgcg cacccgcgac gcgctcgacg tgctctgcga gaacgtccgg atcgcccagg
     5401 acgcgctccc ggtgccgctg gcgctggaga acatcgccgc gctcgtcaac tggcccggcg
     5461 aggagatgac cgagggccgg ttcctgtccg aactcgtcga gcggaccggc gtacggctgc
     5521 tcatcgacgt cgccaacctc catacgaacc acgtcaaccg gggcgaggac ccggccgcga
     5581 ccctcgacgg gcttccggtc gaggccatcg cgtacgtgca tgtcgcgggc ggtgtcgagc
     5641 gcgacggggt gtggcacgac agccacgccc acccggtcac ccagccggtg ctggacgtgc
     5701 tcgccgagct ctgcgcccgg atcgccccgc ccggtgtact gctggagcgc gacgacgact
     5761 tccccgaggg cggcgagctg gccggtgaac tcgacgcgat ccgcgcggtg gtgaaggggg
     5821 gcgtccgtca tgcgcaggcg gcttgagccg gtacgggggg acgaggacgc ggtacgggag
     5881 gagcaagccc cgggacgggc cggcgaaggc accgtcgcgg cggacgcgag ggatgagggg
     5941 gccgagagaa ccgggagaac cgggagggcc gcccgggaaa ccggggacag caagggcgcc
     6001 gcacagggcg gggacgaggc ggtcccggcg gacccggtgg cgccgtcctc gggtgcgacc
     6061 ggcccgcagc cgtcgcccgg tgccggcggg gccgagcggc gcccggcctc ctccacggac
     6121 gccgcgcggg agcggctggc gctggcgcag gccgcgctgc tgtcggcact ggtggcgggg
     6181 acgcccgcgc cggagggctt cgaccacggc cggctgcggg tgcagagccg ggcgctgacc
     6241 gccaagcggg ccgcggtcgt cgccaaggtc gccccggaac tcccggagat cctcggcgac
     6301 gcctaccgtc ccgccttcct cggctacgcc cggtaccgcc ccctgcgcgg cggctaccgc
     6361 caggacgcac tggacttcgc ggcgtacctg ctgacccagg gccgccccga agacccggcc
     6421 gcgcggcggc agttgacgcg ctggtggcgc gagcgcgcgg gtccccggcc gccgtccgcg
     6481 cgaccggtgg cccggctcgc gcgcgccgtg cggcgtgcgc tgcaccgacg ttgagggcca
     6541 ccccgccctt cgtcgtgcag gcccgttcgc cccataggtc cattcgccac gcggctcccc
     6601 gccgccgcat acccgccata cgggcaaccc tggccaccgt cgaggcggtg cgggggccgg
     6661 cggcgtgcgg ggcgggcggc ccggcgccgg gttccggctc gccgcccttg cgggggttcc
     6721 gccgcgggcc cgccgggctc ggcgacgcgg ccttgcgcat ggcgcgccac tcttccgggg
     6781 catagcgtcc gacaccggag cgcggtgctt tacttcgtca ccatccggcc caactatccc
     6841 ttttgtatgc acaccgtgcg gcctagggcc ggcgagcccc tccgggagcc ccgcaccgcg
     6901 cgccacgcac catacgaagg gacggctcgt gagagcactt gcgttgtaca gcgccctcgg
     6961 gtcgctggtc ctgacccccc tcgccaccgc accggcgggt ggtgcggtcc gggaccccgc
     7021 cgcgccgccc gcccccgtcc acccgatctc cttcggccgg tgcgcggcgg tggagcgtct
     7081 gccagccgcc gtcgagtgcg gcaccctcgc cgtgccgctc gactacgcgc gccccaacgg
     7141 caggaagatc cggctcaccg tcagccgggc ccgggccacc gcgcccggcg agcggcaggg
     7201 cgcgctggtc ttcaaccccg gcgggccggg cgcgtccagc atgcagttcc cgctctacgg
     7261 tgggctgccc acctggcggc agaccgcccg ctcctatgac ttcgtcggct acgcaccgcg
     7321 cggggtgggg cgttcggcgc cgctgtcctg ccaggacccc aaggagttca ccaaggcgcc
     7381 gacggacagc ccgaagtacc ccagcgacgc cttcaagcgc cagaaggtgg cggaggcgaa
     7441 ggcgtatgcg cgcggctgcg cgcagcgggc cggtgccgac ctgccgtact acaactccct
     7501 gaacaacgcc cgcgacctgg acatgctgcg ggccgcgctc ggcgagcgga agctgacgtt
     7561 catgggcgcg tcctacggga cgtacttcgg ggcggtctac gcgacgctct tcccgggcca
     7621 tgtccgccgg atgatcttcg acagcatcgt caacccgcag ccgcagcaga tctggtaccg
     7681 caacaacctc gaccagagcg tcgccttcga gagccgctgg agcgactggc tgcgctgggt
     7741 cgccaagcac aacgacacct accacctggg cacgagcaag gcggccgtcc agcgcgcgta
     7801 cgacaccgcc gtcgagcagc tgcgccgcaa gccggtcaac ggcaagatcg gaccggcgca
     7861 gttgcaggcc gcgttcctca agaccggcta caacgacctc ttctggccga tgcgcgccca
     7921 cgcgctgtcg aagtacctgc acggcgacgc caagcagctg atcgcccagg ccatgccctc
     7981 gggcgacggc aaggacgagg agaactccaa cgcggtctac acggcggtcg agtgcaatga
     8041 cgcggcctgg ccgcgcaact ggagcgtctg ggaccgggac aacacccgga tcgcccggac
     8101 cgcgccgttc gagacctggg acaacgtctg gatgaacctg ccctgcgcgt tctggccgga
     8161 ccgggcgcgg tcggcccagg cgggcgtcga cgacgcggtg cgcggcacgg ccgaccgcgc
     8221 ggcgcagggc acgctgggcg ccggggagct ggcggacgcc tcgctgcagg cggcggacga
     8281 cctggacgac acgctgcacg gccggcgccg tccgctcgac atccggaccg agcccggcgc
     8341 gctgcccccg gtgctgctgc tggcggccga gcgggacgcg gccacgccgt acccgggcgc
     8401 gctggagctg cagcggcggc tgccgggctc ctcgctggtc accgagcgga aggcgggcac
     8461 ccacggcatc gcactcggcg gcaacgcctg tgtgaacgcc tacgtcgagg cgtatctggt
     8521 gcgcggcagg gtcccggggc accgggtgta ctgcggcccg cgcgccgagc cggtgccggg
     8581 ccagcagctc cagcgggcgc gtcagctggt gcagcaggcg cagggcaagt agcggccggg
     8641 atccctcggc gcaccacgca cgacgcacgg gccgtccccg agggggcggc ccgtggcgct
     8701 gggtgccggc gggtcggacc ggccggcggg tcaggccagg cccgcgacca gctcggcgac
     8761 cggcttccgg cggcccgtgt agaacgggat ctcctcacgg acgtgcatcc gggcctcgga
     8821 gccgcgcagg tggcgcatca ggtcgacgat gcggtacagc tcgtcggcct cgaaggccag
     8881 cacccactcg tagtcgccga gcgagaacga cggcacggtg ttggcgcgga cgtccgggaa
     8941 gccgcgggcc atcttgccgt ggtcggcgag catccggcgg cggtcctcgt cggggagcag
     9001 gtaccactcg taggagcgca cgaaggggta gacgctcacg taggcgcggg gctcctcgtc
     9061 ggcgaggaag gccgggatgt gcgacttgtt gaactcggcg gggcggtgca gcgccatgtt
     9121 cgaccacacc ggctccaggg cgcggcccag acgggtccgg cggaagaggt tgtacgcctc
     9181 ctggagggcg tccgaactct ccgagtgcca ccagatcatg aggtcggcgt cggcgcgcag
     9241 gccggacacg tcgtaggtgc cgcggaccac cacgtccttg gcggcgagct gggcgaacag
     9301 ctcctcgacc tcgtcggcgt agccgccgcg gtcctcgggg aggacgtcgc gcagcttgaa
     9361 gaccgaccag agggtgtagc ggatgacctc gttgaggtcc ttggccttct taccggcgtt
     9421 ggggatcttt tcgggggcgg tggaagcgtc tgtcatgtgg tccattctca ctcgccttcg
     9481 gcggggaccg ccgccagggt cccgaggatc tcgtcggcgg cgcgtcgcgc cgagccgatg
     9541 caggccggga tgccgacgcc ttcgtagagc gcgccgcaca cccgcagcgg gccgggcgcg
     9601 ccggcgaccg cggcgcggat ccggtccacc cggtcgacgt gcccgacggc gtactgcggc
     9661 agcccgccgt cccagcgggt gaccgtggtg gccaccggcc gggcggccag gccgacggca
     9721 gcgccgaggt cggtgagcga gagctcgacc agctcggcgt cgtcccgctt gaggtcggcg
     9781 tcgtcgccgt acctgccgag ggaggtgcgc agcacgaaca ggtccgggtc ggcccgggcg
     9841 atccagtccc atttgtggct ggcgaaggtc gacgccttga tgcggtggcc gtcgacgggc
     9901 ggtacgagga agccgctgcc gctgggcacg gtgcccaggt cggagcggcg gaaggccatg
     9961 gtgaccagcg ccatcgaggc gtagtcgacg gtttccagct cgtcggccgc ggccgggcag
    10021 ccggcgcgga gcagccgcgc ggcggcgccc gcgggcgccg ccatgatcac cgcgtccgcc
    10081 gcgatccgcc ggtcgccggc cgcgatccgc cagccgtccg cggccgtgtg gcgcagctcc
    10141 cgcacctccg taccgagcag gatctcgccg ccggcacggc gtaccgcgtc ggcgacggcg
    10201 cccggcaggg tcccgatgcc gccgtcgatg cccatgaaga cggagccgcc ggcaaccggg
    10261 ccgccggggg gcacgagggc cgcggcgcgc gcctggatgg cgcgtacgcc ctcgatcagc
    10321 gagcgctcgg tgcgggccgc ctcgaagagc tgcgggacgg cggcgcgcat cgagatgcgg
    10381 tacgcgtcgc ccgcgtacac gccgccgagc agcggctcga cgagccggtc gacgacctcc
    10441 cggccgagcc gtgccgcaac gtactcgccg accgcgacgt ccgtcccggc ctcggtgcgc
    10501 gccagcgtcg aatcctcggc gatccgcgcc atgccctccg gggagatcac accggaggcg
    10561 gcgagcggcg ccaggtcgcc ggggacaccc atgacgtggc ccttgggcat cgggcgcagc
    10621 gctccgcggg tccacagcga cgcggtggcg gtgccgggcg gctggaggcg ctcgccgagc
    10681 ccgacggccc gtgccagttc gaccgcctcc ggccgccggg ccagcatcga ctcggcgccg
    10741 aggtcgaccg gtacgcccgc gatctcgccg gcgagcagct tgccgccgag ccggcccgac
    10801 gcctccagga gggtcacccg ggcgccgccc tccagcaggt ggtgcgcggc ggtcagcccc
    10861 gcgatgcccc cgccgatgac cacgacatga ccgggcgccc ggcccctgct ggtgtgcgct
    10921 gcgctcatgc ctccactctc tcagagtccc ctccgggccc ggaccgtgac ctcgtcgaga
    10981 ggcccggacg gcaacccgct tcgggtaccc ggcgtcgaac cggcgcccag atcaagccag
    11041 ttggcacagc aggccggaat caggagggga cggcgtcgtg cggggatcac ggggcagggg
    11101 gacgggcgcc cggcatcggc gggccggacg gccgggcacc ggagcgcacc cggcggcgat
    11161 gcgcccggcg gaccgggccg gggcggcggg cccggtacct ccggagaagg tggccgagcc
    11221 tgccgttgac agcgggcggt gccgggcaaa gactcggggc ggcgagcggc gatctttcgt
    11281 accgcccgcc ggccgccgag ccgggaccgc gagtgcagcg aggacagaac ctgatgaccg
    11341 acccacgcgt acgggatgtc tacatcgtcg atgcggtccg cacccccgtg ggcaaatacg
    11401 gcggcgcgct ggccccggtc cgccccgac
//
