LOCUS       NZ_CP047146            11032 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. GS7 chromosome, complete genome.
ACCESSION   NZ_CP047146
VERSION     NZ_CP047146.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GS7
  ORGANISM  Streptomyces sp. GS7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9067183)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9067183)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-NOV-2019
            Assembly Method                   :: SPAdes v. Nov-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:53:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,019
            CDSs (total)                      :: 7,925
            Genes (coding)                    :: 7,616
            CDSs (with protein)               :: 7,616
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 107 of 309
            Pseudo Genes (incomplete)         :: 258 of 309
            Pseudo Genes (internal stop)      :: 29 of 309
            Pseudo Genes (multiple problems)  :: 79 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:40:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5674077
            Orig. end                         :: 5685109
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047146.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11032
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="24"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11032
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11032
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6032](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6032
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    541..556
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.75"
                     /tool="antismash"
     gene            complement(1375..3741)
                     /locus_tag="GR130_RS24665"
                     /old_locus_tag="GR130_24665"
     CDS             complement(1375..3741)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537753.1"
                     /locus_tag="GR130_RS24665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_24665"
                     /product="MFS transporter"
                     /protein_id="WP_159506735.1"
                     /transl_table=11
                     /translation="MTQTTTDQLPARAAGASAAPPPSDPGRDAHPRAGGGIVPVLAFAG
                     IVVAVMQTLLVPVIKDLPTLLNTAASNATWVMTATLLAGAVATPIMGRLGDLYGKRRML
                     LTSLAVMVVGSLVCGFTDDLVVMIVGRALQGFAMGAIPLGIGIMRDELPRERLGSAMAL
                     MSSSIGVGGGLALPAAALVAQHADWHALFFGAAGLGVLSIVLIILLVPETAVRAPGRFD
                     VAGALGLSAGLVALLLPITKGSDWGWGSPTTLGLFGAAALILALWGVMELRIADPLVDL
                     RTSARREVLLTNLASITVGVAFYAISLVLPQLLQLPKSTGYGLGQSMVVAGLCVAPLGL
                     TMMFVAPVYARIAARRGPKISLLLGMLVIGIGYGGGIGLMSAPWQTVIIAVVVGAGIGL
                     AYSSLPALIVGAVDPSETGAANGLNTLMRSIGTSVSSAVIGMVLAHMSTRIGPVSVPTM
                     SGFRVSFLIATAAVALGVLVAAFLPSQRTSSRPTPVARHAEGADAADEPAAADAVAATP
                     EPAAERAPAQAPAAGFRGRVVDGAGGPVPRAGVTLIDRQGRQAGVTTADAEGRYALAAP
                     GAGTYVLTGAAPGHTPHAVAATCRGVGAPVETDLILTAAGSRLGGTVLGGPDGTPLAGA
                     SIVVTDARGDVVTRTSSGADGSWAVAQLANGDHTLVLSAPGHQPQARAVQLSGGGPERQ
                     DARLRPNATVRGTVRGPDGQPLRDAAVTLLDDGTVAGHTVTGPDGAFAFADLSSRHYTL
                     AAAGYPPHAAPISLTGGGDAVLDLDLVQPSGAEAG"
     misc_feature    complement(3833..3846)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.73"
                     /tool="antismash"
     gene            complement(4132..4827)
                     /locus_tag="GR130_RS24670"
                     /old_locus_tag="GR130_24670"
     CDS             complement(4132..4827)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657053.1"
                     /locus_tag="GR130_RS24670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_24670"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_159506736.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRNILEAAASVFDDRGYDRATIAEVLERAGVTKGA
                     LYFHFASKEELALAVLEDHVVDIAVAPQKIKLQEFVDSGQVLAYRLRRDPMQRGAARLA
                     VEQGSNRLDRKRSMLAWTRFVEELLTEAKERGEVLDSVVVRETAELFVGAFAGLQTMSH
                     ALTNQADLCHRLAVFFEHTLPSIAVPAVLAKLKLDPERGEKLDLELRGGRTEEGAFLDA
                     GNSLADAAS"
     misc_feature    4926..4939
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     misc_feature    complement(4945..4961)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.12"
                     /tool="antismash"
     gene            5001..6032
                     /locus_tag="GR130_RS24675"
                     /old_locus_tag="GR130_24675"
     CDS             5001..6032
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="GR130_RS24675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_24675"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_159506737.1"
                     /sec_met_domain="AfsA (E-value: 5.4e-21, bitscore: 73.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTLQVSGTIRPRAKRLAQPVRSPRADARPGRGFATRVPREFVH
                     RPFAEDVLITSWRRLDESHFSVTAQWPHNHRYFAPTNGRHHLLLAGETIRQAGLLLSHA
                     ELGVPVGHHFILGDLVYTTDPEQLAVENEPTRLTIDVTCSKTRMRAGSLVSSRFDMTLR
                     KEGRIVATGNSSVSVTSPAVYRRIRGERLAARRTVGPLPTPVPAGLTGSATDGDVLLAP
                     TDRPDHWLLRIAPGHATVVNPANDHVPGLSLLDAAQQAARAVTAPDAFVPYAFGTEFCR
                     YAEHGVPCVIEARRVPSAIPRTTMVQVTGSQNGEPVFTSTLTALDAPGRADAPPAPDVP
                     QAS"
     gene            complement(6020..6748)
                     /locus_tag="GR130_RS24680"
                     /old_locus_tag="GR130_24680"
     CDS             complement(6020..6748)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608010.1"
                     /locus_tag="GR130_RS24680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_24680"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_159506738.1"
                     /transl_table=11
                     /translation="MPATTTRPTAFADRVAAADWTALAGELDSLGCALTPQLLTPADCR
                     ALSGLYDEPGRFRSTVDMARHRFGAGQYRYFADPLPEPVRALREAFYPYLLRTARDWAA
                     RLGRPAPWPDTLADWLAMCHEAGQSKSAQILLRYGPGDWNALHRDLFGELVFPLQVVIG
                     LDAQGTDYTGGEFLLVEQRPRAQSRGTSTVLPQGRGLVFTTRDRPVRSTRGWAAGPVRH
                     GVSAVRSGLRHSLGLVFHDA"
     gene            complement(6786..7295)
                     /locus_tag="GR130_RS24685"
                     /old_locus_tag="GR130_24685"
     CDS             complement(6786..7295)
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608011.1"
                     /locus_tag="GR130_RS24685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_24685"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_159510186.1"
                     /transl_table=11
                     /translation="MTLYATLASPLGELLLVGEESATAPGGVALASLSVPGQKGGATVQ
                     DGWTYDPAAFAGIERQLRAYFDGAPTRFEIPSVTRGTAFQQRVWKALEDIPYGTTTTYG
                     RIAADIGAARGAVRAIGTAIGANPLLVVRPCHRVIGADGSLTGYAGGLERKAQLLALEG
                     ALPAES"
     gene            complement(7310..8860)
                     /locus_tag="GR130_RS24690"
                     /old_locus_tag="GR130_24690"
     CDS             complement(7310..8860)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434162.1"
                     /locus_tag="GR130_RS24690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_24690"
                     /product="DNA-3-methyladenine glycosylase 2 family protein"
                     /protein_id="WP_159506739.1"
                     /transl_table=11
                     /translation="MTEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRRN
                     VRFYPTAAAAQGSGFRACRRCRPDAVPGSAEWNARADVVGRAMRLIGDGVVDREGVAGL
                     ADRLGYSARQVQRQLTAELGAGPVALARAQRAHTARILLQTTGLPVSEIAFAAGFASVR
                     QFNDTIKEIYALTPSALRAARPAGAARPGTGARSAPVAPSATPGVLPLRLPFRGPYAAR
                     QIFDHLQRHALAGIEDVGGAPGRRTYRRTLRLPNGTGVVEVDEATGDGWLECRLRLTEL
                     RDLTTATQRIRRLFDLDADPYAVAGRLGADPVLAPLVARYPGLRSPGAADPQEVAVRAV
                     LGRRMSAAGARTLGGALIAAYGEPLPQPAGALTRLFPRVEDLARAPLTGLGLPEARRAT
                     LRTLSAALADRTVILDAGTDRDEAERALLGLPGIGPWTAGYLRMRALGDPDVLPAGEAR
                     AIEGADPSAWRPWRSYATHYLWTAEAAGDAPDPGAADHRPPHRIDPDRRPDRLTKRTE"
     gene            complement(9252..9479)
                     /locus_tag="GR130_RS24695"
                     /old_locus_tag="GR130_24695"
     CDS             complement(9252..9479)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="GR130_RS24695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_24695"
                     /product="hypothetical protein"
                     /protein_id="WP_159506740.1"
                     /transl_table=11
                     /translation="MTEHPPVIVHPPTPSGGRRVTVHGQIVGLAHGRGDVAEFLRRAGV
                     ADPAEDIALDDPRLVEWRGGSLDDWPVPRA"
     gene            9674..10279
                     /locus_tag="GR130_RS24700"
                     /old_locus_tag="GR130_24700"
     CDS             9674..10279
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971478.1"
                     /locus_tag="GR130_RS24700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_24700"
                     /product="HD domain-containing protein"
                     /protein_id="WP_159506741.1"
                     /transl_table=11
                     /translation="MTMDDLVIPETTACRAAREVASAYCSPALLNHSVRAYLWAAGYAS
                     AHGIAFDAELLYVAAMFHDIGLVKEFDSHTVPFENAGGHIAWVFAAGAGWPVERRVRAS
                     EVIVRHMWDEVDVATDPESHLLAFSTSLDISGRRPDALPASLRNEVLDRYPRLGLGEEF
                     LACFRDQAERKPDSAAAECVRAGIADRIAANPLDAHGA"
     misc_feature    complement(10461..10476)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.39"
                     /tool="antismash"
     misc_feature    complement(10712..10727)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.75"
                     /tool="antismash"
ORIGIN
        1 tgcgatccgc cggcctcggg cacccggtgg gtgacgtcgc cttcggggcg gtagccgctg
       61 ggtccggtgt tccacacccg cacgatgtcg ccggggccgt cgccgaagtt ctccagccgg
      121 ccctcggtgc cgatgacggt gtagttgcgc cagtagtcgg gagtgaagtg gcactgctgg
      181 taggcggcga gcaccccgtt gtcgagctgc atgttcatca cggacgcgtc ctcgacgtcc
      241 accacgtggt gcagatcccg gcgggcggtc ggcggccagt cgtactcccg cagccagccg
      301 tccgggcgcg gggtgccggg ggcgcggcgc ggcaggtcgc cgtagacgag gaggtcgccg
      361 agtgcgttga cgcggtgggt gtagccgccg gcgagcagat ggatcacgtc gatgtcgtgg
      421 gctgccttct ggagcagcag gccggtggtg cggcggcggt cggcgtgcca gtccttgaag
      481 tagtagtcgc cgccgtacgc gacgaagtgc cgggtccaca ccatcttggg ctcgccgatg
      541 tcgccgcggg cgatcagacg gtgcatcagc cggaccacgc ccatgtgccg catgttgtgg
      601 ccgacgtaga ggcgggtgcc ggtacgccgg gcggtgcgca ggatggtgtc gcagctctcg
      661 gcggtgatac cgagcggctt ttcgacgtag accgggcggc ccgccaccag agcgtcgcag
      721 gcgatgcgct catgggtgtc gtcgggggtg gcgacgacaa ccgcgtcgac ccagtcgtcg
      781 gcgaggagtt ggcggtggtc gccggtggcg aggcgggcgc cgaagcgggc ggccgcgcgg
      841 cggcggacgc cggggtcgag atcggcgacg gcggtgaccg cggagccccg gccgggccgg
      901 tgcgcggcgt ccgccaggtt gccgcgcatg ccgagtccga tgacgccgat gccgaggtcc
      961 gctgccacgg tgtctcctgt ccgctcgccc gtccgtgggt gcaccggcgc tccggtgcac
     1021 ccgactgccg gtctgccccg gtgccggccg gcttacgccc cgggggccgg ggcggacagg
     1081 atgcggcggt cgacggccgc cgggacggcc cgcaggacgg cccgcaggga cacgtcggcg
     1141 cggaggtgtc ctggcccgcg cccacggtcg cggcgacgcc cggcacacgg ccctggtgga
     1201 ggccgacgcg agcggtggcg ccgaggcggc cggccgctcg ctccgggacg aaccggccga
     1261 aaggcgggcg cacttgacgg tgcggtgagc cgtaccgccc ggcggcgggc cggggcgtgt
     1321 tggcacggtg cgggcccggc gcacacgggc ggcgcgcggg cccgccggcg gtcgtcagcc
     1381 cgcctcggca cccgacggct ggacgaggtc cagatccagc accgcgtcgc cgccgccggt
     1441 gagggatatc ggcgcggcgt gcggcggata gccggccgcc gcgagggtgt agtgacggct
     1501 gctcagatcg gcgaacgcga aggcgccgtc cgggccggtg accgtgtgtc cggcgaccgt
     1561 gccgtcgtcg aggagggtca cggcggcgtc ccgcaggggc tgcccgtccg ggccgcggac
     1621 cgtgccgcgc acggtggcgt tcggccggag ccgggcgtcc tggcgctccg ggccgccgcc
     1681 ggacagctgg acggcacggg cctgcggctg gtggccgggg gcgctgagca cgagggtgtg
     1741 gtcaccgttc gccagctggg cgacggccca actgccgtcg gcgccggatg acgtacgggt
     1801 taccacatcg ccccgggcgt cggtgacgac gatgctcgca ccggcgagcg gggtcccgtc
     1861 cggaccgccg agcacggtcc cgccgaggcg gctgccggct gcggtcagta tcaggtccgt
     1921 ctcgacgggc gcgcccacgc cgcggcaggt ggccgcgacc gcgtgcgggg tgtggcccgg
     1981 cgcggcgccg gtgagcacgt acgtgcccgc acccggggcg gcgagcgcat agcgcccctc
     2041 ggcgtcggcc gtggtgacac cggcctgccg cccctgccgg tcgatcagcg tgacgccggc
     2101 gcgcggcacc ggcccgccgg ccccgtcgac gacccgcccg cggaacccgg ccgcgggtgc
     2161 ctgcgccggg gcgcgctccg ccgccggctc cggcgtggcc gccaccgcgt cggccgcggc
     2221 gggctcgtcg gccgcgtccg ccccttccgc gtgccgggcg accggcgtcg gccgggacga
     2281 ggtccgctgg gacggcagga aggccgccac cagtacgccg agggcgaccg ccgcggtggc
     2341 gatcaggaag gagacccgga agccggacat ggtgggcacc gagaccgggc cgatgcgcgt
     2401 cgacatatgg gcgagcacca tgccgatcac ggcgctggac acggatgtgc cgatcgagcg
     2461 catcagggtg ttgaggccgt tcgccgcgcc ggtctcggag gggtcgacgg cgccgacgat
     2521 cagcgccggc agcgaggagt aggccagccc gatgcccgct ccgacgacca ccgcgatgat
     2581 gaccgtctgc cagggggcgc tcatcaggcc gatgccgccg ccgtagccga tcccgatgac
     2641 cagcatgccc agcagcagcg agatcttggg gccgcgccgg gcggcgatcc gcgcgtacac
     2701 cggggcgacg aacatcatgg tcaggcccag gggcgccacg cacagaccgg ccaccaccat
     2761 ggactgaccc aagccgtagc cggtcgactt cggcagctgg agcagctgcg gcagcaccag
     2821 cgagatggcg tagaacgcca caccgaccgt gatcgaggcg aggttggtga gcagcacctc
     2881 gcgccgcgcg ctggtccgca ggtcgaccag cggatcggcg atccgcagct ccatcacgcc
     2941 ccacagcgcc aatatcagcg ccgcggcccc gaacaggccg agggtggtcg gcgaacccca
     3001 gccccagtcg ctgcccttgg tgatcggcag cagcagggcg acgagcccgg cggacagccc
     3061 gagggcaccg gcgacgtcga agcggcccgg ggcccgcacc gcggtctcgg ggacgaggag
     3121 gatgatgagc actatcgaca gcacaccgag cccggcggcg ccgaagaaca gggcgtgcca
     3181 gtcggcgtgt tgggccacca gggcggcggc cggcagcgcc agcccgccgc cgacgccgat
     3241 cgacgaactc atcaacgcca tggcggagcc gagccgttcg cgcggcagct cgtcgcgcat
     3301 gatgccgatg ccgaggggga tcgcgcccat ggcgaagccc tggagcgccc gcccgacgat
     3361 catgacgacc aggtcgtcgg tgaacccgca gaccagcgag ccgacgacca tgacggcgag
     3421 gctggtgagc agcatccgcc gcttgccgta caggtcgccg aggcggccca tgatgggcgt
     3481 tgcgacggca ccggcgagga gggtggccgt catgacccat gtggcgttgc tggccgcggt
     3541 gttgagcagc gtcggcaggt ccttgatgac cggcacgagc agcgtctgca tcaccgcgac
     3601 gacgatgccg gcgaaggcga ggacggggac gatgccgccg cccgccctgg ggtgggcgtc
     3661 cctgcccggg tcggacgggg gcggcgcggc ggaggcgccg gccgcccttg ctggtagctg
     3721 gtccgtcgtc gtctgggtca tgcgggcggc ctccagtgcg gcaggggtga gcggagaggg
     3781 gtgcgcggtg agcgtcgtga aggggagtgc cggccccctg gtacgtgcat gccgaacgtt
     3841 ttatctcggg gcgctattcc gccacacgtc ccaccgtctc gcccgcccgc cggtgatctc
     3901 cctcactccc gccgacctgc atcgcggccc gctcgccggc ccgttcatgg cccctccccc
     3961 ggtctcgttc cggacaccga ctccagtgaa ccgcatgcca ctgacaatcg ccccgggtcg
     4021 ccccgggacc gcggtcagga ccgcacgccc ggtatggttc ggccggctga ttcgcccgtg
     4081 tcgcccctct ggatcccgcc ctttccgact cgcgggcaac agcccgaatt cctacgacgc
     4141 cgcgtccgca agggagttgc ccgcgtccag gaacgcgccc tcttccgtgc gcccgccccg
     4201 gagttccagg tcgagcttct cgccgcgctc cgggtccagc ttcagcttgg cgaggaccgc
     4261 gggcaccgcg atgctgggca gggtgtgttc gaagaaaacg gccagccggt ggcagaggtc
     4321 ggcctgattg gtcagcgcgt gcgacatcgt ctggaggccg gcgaacgccc cgacgaacag
     4381 ctcggccgtc tcccgtacga ccacgctgtc cagcacttcg ccgcgctcct tcgcctcggt
     4441 cagcagctcc tcgacgaacc gggtccacgc cagcatcgac cgcttgcggt caaggcggtt
     4501 ggagccctgc tcaacggcga ggcgggcggc gccgcgctgc atggggtcgc gccgcagccg
     4561 gtatgccagg acctggcccg agtcgacgaa ctcctgcaac ttgatcttct gcggcgccac
     4621 cgcgatgtcc accacgtggt cttcgagcac ggcgagggcg agttcctcct tcgacgcgaa
     4681 gtggaaatag agggcgccct tggtgacacc ggctcgctcg agaacttcgg cgatggtcgc
     4741 gcggtcgtag ccacggtcgt cgaaaaccga cgccgcggct tccaagatgt tccgccgggt
     4801 tctgatcgcg cggtcctgtt gcgccacagg acgcctcctg tctctcgaag cacacgggtg
     4861 tttccccagt gggttgtcgc ccaagtctcc cgcagatcgg ccgttggctc gaatttcctc
     4921 gtcggcattg aaaagaaaac cggatggtac gtatcttacc agcgtcgtac ctcttcccgt
     4981 tgagacgcct gggggcagtc atgagcgaca cgttgcaggt cagcggcacg attcggcccc
     5041 gggccaagcg cctggcgcag ccggtccgga gccctcgcgc cgacgcccgg cccggtcggg
     5101 gattcgccac ccgcgtaccg cgcgagttcg tgcaccgtcc gttcgccgag gatgtcctga
     5161 tcacgtcgtg gcgccggctg gacgagagcc acttctcggt caccgcccag tggccgcaca
     5221 accaccggta cttcgccccg accaacggcc ggcaccacct cctcctcgcc ggcgagacca
     5281 tccgccaggc ggggctgctg ctctcgcacg ccgaactcgg cgtgccggtc ggccaccact
     5341 tcatcctcgg ggacctcgtc tacacaaccg accccgagca gctcgccgtt gagaacgagc
     5401 cgacccggct gaccatcgac gtcacgtgca gcaagacgcg gatgcgggcc ggttcgctgg
     5461 taagcagccg gttcgacatg accctccgca aggagggccg gatcgtcgcg acgggcaatt
     5521 ccagcgtgag cgtcacctcg cccgcggtct accgccggat ccgcggcgag cgcctggcgg
     5581 cacgccgtac cgtcggcccg ctgcccacgc ccgtcccggc cggcctgacc ggcagcgcca
     5641 ccgacggcga tgtgctgctc gcccccaccg accggcccga ccactggctg ctgcgcatcg
     5701 cccccggcca tgccacggtc gtcaacccgg cgaacgacca cgtgcccggc ctgtcgctgc
     5761 tggacgccgc ccagcaggcc gcccgcgcgg tgaccgcccc cgacgccttc gtcccctacg
     5821 ccttcggcac ggagttctgc cggtacgcgg aacacggcgt cccgtgcgtg atcgaggccc
     5881 gccgggtccc gtcggcgatc ccccgcacca cgatggtcca ggtcaccggc tcgcagaacg
     5941 gtgagccggt cttcacctcg acactcacgg cactggacgc gcccggccgg gcggacgcgc
     6001 ccccggcgcc ggacgtgcct caggcgtcgt gaaagaccag ccccagggag tgccgcaggc
     6061 ccgagcggac ggcgctcacg ccgtggcgca ccggacccgc cgcccagccg cgggtggagc
     6121 gcaccgggcg gtcccgggtg gtgaagacca gcccgcggcc ctgcgggagc acggtcgagg
     6181 tgccgcggga ctgggcgcgc gggcgctgct cgacgagcag gaactcgcca ccggtgtagt
     6241 cggtgccctg cgcgtcgagc ccgatgacca cctggagcgg gaagaccagc tcgccgaaga
     6301 ggtcgcggtg cagggcgttc cagtcgcccg gcccgtaacg cagcagaatc tgggcggact
     6361 tggactggcc ggcctcatgg cacatcgcca gccagtcggc gagcgtgtcc ggccaggggg
     6421 ccggtcggcc gagccgggcc gcccagtcgc gggcggtccg cagcaggtac gggtagaacg
     6481 cctcgcgcag ggcccggacg ggctcgggga gcgggtcggc gaagtagcgg tactggccgg
     6541 cgccgaaacg gtgccgtgcc atgtcgacgg tggagcggaa gcggccgggc tcgtcgtaca
     6601 ggccgctcag cgcgcggcag tcggccgggg tgagcagctg cggcgtcaac gcgcagccca
     6661 gggagtcgag ttcgccggcg agggccgtcc agtcggcggc ggccacgcgg tcggcgaagg
     6721 cggtggggcg ggtcgtggtg gcgggcatgg tggtgtgctc cagaggggcg gaacggtcgg
     6781 gcgggtcagc tctcggccgg cagggcgcct tcgagcgcga gcagttgcgc cttgcgttcc
     6841 aggccgcccg cgtagccggt cagcgacccg tcggcgccga tgacgcggtg gcagggccgg
     6901 acgaccagca gggggttggc gccgatggcc gtgccgatgg cgcgtaccgc gccgcgcgcc
     6961 gcgccgatgt cggcggcgat ccgcccgtag gtggtcgtgg tgccgtacgg aatgtcctcc
     7021 agcgccttcc agacgcgctg ctggaaggcg gtaccgcggg tgacggacgg gatctcgaag
     7081 cgggtcggcg cgccgtcgaa gtaggcccgc agctgccgct cgatcccggc gaacgcggcc
     7141 gggtcgtacg tccagccgtc ctggacggtc gccccgccct tctggcccgg tacggacagt
     7201 gaggcgagtg cgacaccgcc cggagcggtg gccgattcct cgcccaccag gaggagttcg
     7261 ccgaggggac tggcgagagt ggcgtaaagg gtcagggtca tgggtccggt cattccgttc
     7321 gcttcgtcag tcggtcgggg cgtcggtcgg ggtcgatgcg gtgcggtggc cggtggtctg
     7381 ccgctccggg atccggtgcg tcgcccgcgg cttcggccgt ccagaggtag tgcgtcgcgt
     7441 acgagcgcca cgggcgccag gccgacgggt cggcgccctc gatcgcccgc gcctcgccgg
     7501 ccggcaggac gtccgggtcg cccagcgccc gcatgcggag gtagccggcg gtccaggggc
     7561 cgatgcccgg gaggccgagg agggcgcgtt ccgcctcgtc gcggtcggtt ccggcgtcca
     7621 ggatcaccgt gcggtcggcg agcgcggcgc tgagggtccg cagcgtcgcg cggcgggcct
     7681 cgggcaggcc gagtccggtg agcggcgccc gcgccaggtc ctcgacccgg gggaagagac
     7741 gggtgagcgc gccggccggc tgcggcagcg gctcgccgta cgcggcgatc agtgcgccgc
     7801 cgagcgtacg ggcgccggcg gccgacatcc ggcggccgag cacggcgcgg acggcgacct
     7861 cctgcgggtc cgcggcgccc ggggagcgca gacccgggta gcgggccacc agcggggcga
     7921 gcacgggatc cgcgcccagc cgcccggcga ccgcatacgg gtcggcgtcc aggtcgaaca
     7981 ggcggcggat ccgctgcgtg gcggtggtca gatcgcgcag ttcggtgagg cgcagccggc
     8041 actccagcca accgtctccg gtggcctcgt cgacctccac gacgccggtg ccgttcggga
     8101 ggcgcagcgt acgccggtag gtgcggcggc cgggcgcgcc gccgacgtcc tcgatcccgg
     8161 cgagcgcgtg ccgctggagg tggtcgaaga tctgccgcgc cgcgtacgga ccgcggaacg
     8221 gcagccgcag cgggaggaca ccgggcgtgg cggacggtgc gacgggggcg gaccgggcgc
     8281 ccgtgccggg gcgggcggca ccggcgggac gggccgcgcg cagggcgctc ggggtcagcg
     8341 cgtagatctc cttgatcgtg tcgttgaact gccggacgct ggcgaagccc gccgcgaagg
     8401 cgatctcgga gaccggcagt ccggtggtct ggagcaggat ccgcgcggtg tgggcgcgct
     8461 gcgcgcgggc cagcgcgacc ggcccggcgc ccagctcggc ggtcagctgc cgctgcacct
     8521 gccgggcgct gtagccgagc cggtcggcca gaccggcgac gccctcgcgg tcgaccaccc
     8581 cgtcgccgat cagccgcatc gcccggccta cgacgtcggc ccgcgcgttc cactcggccg
     8641 agccgggcac cgcgtccggg cggcagcgcc ggcaggccct gaatccggag ccctgggccg
     8701 ccgccgcggt gggatagaag cggacgttcc gccgccgcgg ggtggtcgcg gggcagctcg
     8761 gccggcagta gatcccggtc gtggacacgg cgaagaagaa ggcgccgtcg aacctggcgt
     8821 cccggctccg caccgcctcg tacctgctgt cctcggtcat cacacgaccc actgtgcggc
     8881 actcggagcc ccggtgctgg cggaaatcgg acaccgcggt gggcgcgcga cggccatggg
     8941 gcggagccag ggatccggtc gcggcctcgg tgggcgagcc gcctgccgct ccggtggctc
     9001 gggtcatcga cgtccggtgg ctccggcctt cgacccactt gctggtggct ccggtcatcc
     9061 acccgcactc ggcgcgcggc gaccgcccgc ctgtcccatc gccacggacg aggtcccccc
     9121 atcccgggcc ccttcgcacg aggtcccgtc gcccccccgg cggcaacatc gagccgccac
     9181 cgtcccgtcc gtacaactcc gctgcgctcg cgctcagttc ggaacagtgg agccccagac
     9241 cgcccgacgg ttcacgcgcg cggaaccggc cagtcgtcca gcgagccgcc gcgccattcg
     9301 accaggcgcg gatcgtccag cgcgatgtcc tcggcgggat cggcgacgcc cgcgcggcgc
     9361 aggaattcgg ccacatcccc gcggccgtgg gccaggccga cgatctgccc gtgcacggtc
     9421 acccgacggc ccccggaggg cgtcggcgga tgcacgatga caggcggatg ctcggtcatg
     9481 cctccagcgt gcgccgggct ccgcgcgctc gcacctcggc ggcgggtggg cgggcgggtc
     9541 gccggtcacc gggtcggtca cggagacggt cgcagcaggg gtggcgacag gggcggtggc
     9601 agcgcatacg gcggacggcc gagggtgcca ccgcccatgc ccgaggggcc gacggtaggc
     9661 cacaatcacg gccatgacca tggacgatct tgtgataccc gagaccaccg cctgccgtgc
     9721 cgcgcgggag gtggcgagcg cctactgctc gcccgcactg ctgaaccact ccgtacgggc
     9781 ctacctctgg gcggccggat atgcgtcggc ccacggcatc gccttcgacg cggaactcct
     9841 ctacgtggcc gcgatgttcc acgacatcgg gctggtcaag gagttcgaca gccacaccgt
     9901 gcccttcgag aacgccggcg ggcatatagc gtgggtgttc gccgcgggcg ccgggtggcc
     9961 cgtggagcgc cgggtgcggg cgtccgaggt gatcgtccgg cacatgtggg acgaggtgga
    10021 cgtggcgacg gacccggagt cgcatctgct ggcgttcagc acgtcgttgg acatctccgg
    10081 gcggcgtccg gacgccctcc cggcgagcct gcgcaacgag gtgctggacc gctacccgcg
    10141 gctgggcctc ggcgaggagt tcctcgcctg cttccgggac caggcggagc ggaaaccgga
    10201 cagtgccgcc gcggagtgcg tacgcgccgg catcgccgac cggatcgccg ccaatcccct
    10261 ggacgcgcac ggggcgtagg cgcggactgc ggccatccct cccagcgtcc agcagacgga
    10321 aacggagaag gagacggaga cggcagaaaa atgccccggc cagacggggg agactggccg
    10381 gggcggcttg gcggaggcgt ttctcaactg cccacactcc attgaacggt gaacagagga
    10441 tgagcgttcc atgaaggata cgtgacggtc atcacagagg ccatcaacgg ggacgttcaa
    10501 gacagtcaac cgggacagtg agtgggcggg cggcggacgg tgcggagccc gggacgccgc
    10561 cgtgggagcc acatgtacgc gtatacgcgg tctacgcgta ccggcgtacg cgcgaaccgg
    10621 cgccgcgact gcagtgcggc agtcgcggcg ccggttgtcg gtggcggtct gcggcgcgtg
    10681 ccgcggaccg gttcagccct gggtggggtg tccggagagg cgttcgacgc cgcggaggag
    10741 ggcggagtgg tcgaggccgc cgtcgccttg tgcgcgcagg gaggcgacga gggtggcgac
    10801 gacggtgccg acggggaggg cggcgccgac gttgcgggcg gcgtcggtga cgatgcccat
    10861 gtccttgtgg tgcaggtcga tgcggaagcc gggcttgaag tcgcggcgca ggaagttgtc
    10921 cttcttgcgg gcaaggacgg tggagccggc gagtccgccg cccaggacgt cgagggcggc
    10981 gttgaggtcg acgccggact tctccaggaa gacgacggct tcggcgcagg ct
//
