LOCUS       NZ_CP047146            10216 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. GS7 chromosome, complete genome.
ACCESSION   NZ_CP047146
VERSION     NZ_CP047146.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GS7
  ORGANISM  Streptomyces sp. GS7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9067183)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9067183)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-NOV-2019
            Assembly Method                   :: SPAdes v. Nov-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:53:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,019
            CDSs (total)                      :: 7,925
            Genes (coding)                    :: 7,616
            CDSs (with protein)               :: 7,616
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 107 of 309
            Pseudo Genes (incomplete)         :: 258 of 309
            Pseudo Genes (internal stop)      :: 29 of 309
            Pseudo Genes (multiple problems)  :: 79 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:40:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3403542
            Orig. end                         :: 3413758
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047146.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            48..1076
                     /gene="tdh"
                     /locus_tag="GR130_RS14835"
                     /old_locus_tag="GR130_14835"
     CDS             48..1076
                     /EC_number="1.1.1.103"
                     /GO_function="GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tdh"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922312.1"
                     /locus_tag="GR130_RS14835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_14835"
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="WP_159505171.1"
                     /sec_met_domain="ADH_N (E-value: 9.1e-31, bitscore: 104.8,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 3.9e-23, bitscore:
                     80.7, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKALVKQKAEPGLWLTDVPEPAIGAGDVLIKVLRTGICGTDLHIR
                     SWDGWAQQSINAPLTIGHEFVGEVVETGRDVAEIHAGDLVSGEGHLVCGKCRNCLAGRR
                     HLCRATVGLGVGRDGAFAEYVALPAANVWVHRVPVDLDIAAVFDPFGNAVHTALSFPLV
                     GEDVLITGAGPIGIMAAAVARHAGARNVMITDVSEQRLELARKVGVSLALDVGRSSIAD
                     GQRQLGLREGFDVGLEMSGRPEALRDMIGNMTHGGRIAVLGLPSQEFPVDWSRIVTSMI
                     TIKGIYGREMFETWYAMSVLLEGGLDLAPVITGRYPYQEFDAAFAEAAGGQGGKVILDW
                     AA"
     gene            1118..2311
                     /locus_tag="GR130_RS14840"
                     /old_locus_tag="GR130_14840"
     CDS             1118..2311
                     /EC_number="2.3.1.29"
                     /GO_function="GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (45-388). E-value: 3e-71.
                     Score: 232.4. Matches aSDomain:
                     nrpspksdomains_GR130_RS14840_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109: 8-amino-7-oxononanoate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386190.1"
                     /locus_tag="GR130_RS14840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_14840"
                     /product="glycine C-acetyltransferase"
                     /protein_id="WP_159505172.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.4e-69, bitscore:
                     232.2, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFDSVREDLRTTLDEIRAAGLHKPERVIGTPQSATVAVTAGGRPG
                     EVLNFCANNYLGLADHPEVVAAAHEALDRWGYGLASVRFICGTQEVHKELERRISGFLG
                     QEDTILYSSCFDANGGVFETLLGPDDAVISDALNHASIIDGIRLSKARRLRYANRDMAD
                     LERQLKEAEGARRTLVVTDGVFSMDGYLAPLREICDLAERYGAMVMVDDSHAVGFVGPG
                     GRGTPELHGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQRSRPYLFSNSLAPV
                     IAAASLKVLDLLESAGDLRERLHANTALFRSRMTEEGFEILPGEHAIAPVMIGDAAEAG
                     RMAELLLERGVYVIGFSYPVVPQGQARIRVQLSAAHSTDDVHRAVDAFVAARAALAR"
     aSDomain        1253..2281
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR130_RS14840_Aminotran_1_2.1"
                     /evalue="3.00E-71"
                     /label="GR130_RS14840_Aminotran_1_2.1"
                     /locus_tag="GR130_RS14840"
                     /protein_end="388"
                     /protein_start="45"
                     /score="232.4"
                     /tool="antismash"
                     /translation="EVLNFCANNYLGLADHPEVVAAAHEALDRWGYGLASVRFICGTQE
                     VHKELERRISGFLGQEDTILYSSCFDANGGVFETLLGPDDAVISDALNHASIIDGIRLS
                     KARRLRYANRDMADLERQLKEAEGARRTLVVTDGVFSMDGYLAPLREICDLAERYGAMV
                     MVDDSHAVGFVGPGGRGTPELHGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQ
                     RSRPYLFSNSLAPVIAAASLKVLDLLESAGDLRERLHANTALFRSRMTEEGFEILPGEH
                     AIAPVMIGDAAEAGRMAELLLERGVYVIGFSYPVVPQGQARIRVQLSAAHSTDDVHRAV
                     DAF"
     gene            2363..3271
                     /locus_tag="GR130_RS14845"
                     /old_locus_tag="GR130_14845"
     CDS             2363..3271
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091736.1"
                     /locus_tag="GR130_RS14845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_14845"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_159505173.1"
                     /transl_table=11
                     /translation="MIEARRLHILRALADHRTVTAAAAALYLTPSAVSQQLAALEQETG
                     HRLVERGARGVRLTAAGDILLGHANAVLAQLERAESELAAYEAGEAGTVTVAAFATGIG
                     LVLAPAITELARSAPGIRVRVQDAEGDASLLMVLDRQVDVAVAVEYRGAPGEDDPRLTR
                     VPLYAEPFDAVLSPGHPLAGAERVPLAGLAKDPWIGPYSGNPCHDVVVLACEYAGFEPR
                     LEHSSDDFRAVVALASAGAGVALVPRSALRGMDLTDAVVRPVDGVAPTRRVFAAVRRGA
                     EDHPLLRPVLTALRTAAAAQG"
     gene            complement(3285..4325)
                     /locus_tag="GR130_RS14850"
                     /old_locus_tag="GR130_14850"
     CDS             complement(3285..4325)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540970.1"
                     /locus_tag="GR130_RS14850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_14850"
                     /product="PHP domain-containing protein"
                     /protein_id="WP_159505174.1"
                     /transl_table=11
                     /translation="MDPVAALERIAFLLERQGAVTYRVQAFRTAASVVGELPAEELRER
                     AANGTLGRLKGLGPKTTRVVEEALAGRQPAYLARLAEEAGGPLVEGEQGRALLRALRGD
                     CHLHSDWSDGGSPVEAMARAARDLGHDWCVLTDHSPRLTVARGLTAERLRLQLALVAVV
                     NAQLAPFRLLTGIECDILDDGSLDQEPELLDALDVVVASVHSKLRMPKGPMTRRMLAAV
                     HNPLVDVLGHCTGRQITGKPRPESEFDAGEVFAACAEHRTAVEINCRPDRFDPPRRLLR
                     QALDAGTLFSIDSDAHAPGQLDWQIYGCARAEECGVPTERVITTWTARQLATWTRTGRT
                     PHRAAG"
     misc_feature    4460..4474
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
     misc_feature    complement(4510..4527)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.44"
                     /tool="antismash"
     misc_feature    4611..4629
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     misc_feature    4777..4792
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.99"
                     /tool="antismash"
     misc_feature    4968..4982
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.4"
                     /tool="antismash"
     misc_feature    complement(4969..4982)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.98"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="GR130_RS14855"
                     /old_locus_tag="GR130_14855"
     CDS             5001..5216
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576071.1"
                     /locus_tag="GR130_RS14855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_14855"
                     /product="type A2 lanthipeptide"
                     /protein_id="WP_159505175.1"
                     /sec_met_domain="strepbact (E-value: 2e-18, bitscore: 65.5,
                     seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNAQVQTQEISDADLDNVSGGLVGGVLSTVTTTTDSVPQVAGAL
                     TTVTSLVQGASGVDLNGTVGGLTAGL"
     gene            5432..6238
                     /locus_tag="GR130_RS14860"
                     /old_locus_tag="GR130_14860"
     CDS             5432..6238
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="GR130_RS14860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_14860"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_159505176.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEELDLPVRFARPQGWLVLIVTVLVMAGAAVW
                     AFAGTVSSTLGAPAILTHGQGSYVLQSPVAGQVTSVLAEEGKQVAANAPVVKVRTAQGT
                     TTVVRAIAAGRLTTLSATIGSVVTTGADLASVEKTAGAGDRLTATLYVPADSAATLPLG
                     AAVDLSVQSAPTQQYGVLRGKIAAIGRNTQTRQRISAFLGSDQLGQQFSQHGQPVAVLV
                     TLDTAHTASGYAWSTSDGPPFALDSMTQATGAVHLAAQHPIDWLLP"
     gene            6235..8523
                     /locus_tag="GR130_RS14865"
                     /old_locus_tag="GR130_14865"
     CDS             6235..8523
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540973.1"
                     /locus_tag="GR130_RS14865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_14865"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_159505177.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1e-37, bitscore:
                     127.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPNDSAAPPQHLPPAGRGRHRPEPAPAARRRKGPPPTAKTGKA
                     GKSVRTPTVLQMEAVECGAASLAMVLAHYGRHVPLEELRIACGVSRDGSRASNLLKAAR
                     SYGLQAKGMQMEPAALAEVSAPAILFWEFNHYVVYDGMGRRLGRRGVHINDPDKGRRFV
                     AMEDFDTSFTGVALVFEPTDAFRKGGRKPSVLSALPARLRGTTGTLLAALLASLLLVGV
                     GAAVPALSRTYIDMFLIGNQTSLLGPLFAAMTAMVALTAVLTGLQQANLLRGRIISSTL
                     SSARFLRHLLRLPVTFFAQRSPADLVQRLQSNDAVAETLARDLAAAAVDGVVVVLYALL
                     LWTYDPQLTVIGVLIALLNVVAMRIVVRLRATHVQKLRADTARLTNTSYTGLQLIETMK
                     ATGGESGYFRRWAGQHASTLEEQQRLGVPSAWLAVVAPTLATLNSALILWIGGLRAVEG
                     HISIGLLVAFQALVTRFTAPVTRLNGVAGRIQDFAADVARLKDVESFPADTLYSRPEAD
                     AGTRRLKGHVTLEDITFGYSPLDKPLLSGFSLAVGPGRQVALVGGSGSGKSTVSRLISG
                     LYAPWEGTIRIDGQRLEDIPRSVLAASVSFVDQDIFLFEGTVRDNVALWDPSIPDDAVI
                     AALQDAALYDDVIARRPDGIHSRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTS
                     ALDAETEAVIMDNLRRRGCACVVIAHRLSTVRDSDEIVVLDHGVVVERGRHQDLVAAGG
                     AYAELVKEH"
     misc_feature    9449..9463
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     misc_feature    complement(9755..9768)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.58"
                     /tool="antismash"
ORIGIN
        1 gcccccgcga cggcgcggca acctcttcac gtacgggagc tctaacggtg aaggcactgg
       61 tcaagcagaa ggcggagccg ggactctggc tcacggacgt gccggagccc gcgatcggcg
      121 cgggggacgt gctgatcaag gtgctgcgca ccggcatctg cggcacggac ctgcacatcc
      181 ggtcgtggga cggctgggcc cagcagtcca tcaacgcccc actgacaatc ggccacgaat
      241 tcgtcggcga ggtcgtcgag accggccggg acgtggccga gatacacgcc ggggacctgg
      301 tcagcggcga gggccatctg gtctgcggca agtgccgcaa ctgcctggcc gggcgccgcc
      361 acctgtgccg ggccacggtc gggctcggcg tcggccggga cggcgcgttc gccgagtacg
      421 tggcgctgcc ggcggccaac gtgtgggtgc accgggtccc ggtcgacctc gacatcgccg
      481 cggtcttcga ccccttcggc aacgccgtgc acaccgcgct ctccttcccc ctggtcggcg
      541 aggacgtact gatcaccggg gccgggccga tcgggatcat ggcggcggcg gtcgcccggc
      601 acgccggcgc ccgcaacgtg atgatcaccg acgtcagcga gcagcgcctc gaactggccc
      661 ggaaggtcgg cgtcagcctc gccctcgacg tcgggcggtc ctcgatcgcg gacggccagc
      721 gccaactggg cctgcgcgag ggcttcgacg tcggcctgga gatgtccggc cgccccgagg
      781 cgctgcgcga catgatcggc aacatgacgc acggcggccg gatcgccgta ctgggcctgc
      841 cgtcccagga gttcccggtc gactggtccc ggatcgtcac ctccatgatc accatcaagg
      901 gcatctacgg ccgggagatg ttcgagacct ggtacgcgat gtcggtcctg ctcgaaggcg
      961 gcctcgacct cgcaccggtg atcaccggcc gctatccgta ccaggagttc gacgccgcct
     1021 tcgccgaagc ggccggcggc cagggcggca aggtcatcct cgactgggcc gcctgatccc
     1081 cggcccggtc cccccaacgc acccctaagg aaggcccatg ttcgactccg tacgcgagga
     1141 cctgcgcacc accctcgacg agatccgcgc cgccgggctg cacaagccgg agcgggtcat
     1201 cggcaccccg cagtccgcca ccgtcgcggt caccgccggc ggccgtcccg gcgaggtcct
     1261 caacttctgc gccaacaact acctgggcct ggccgaccac cccgaggtcg tcgccgccgc
     1321 ccacgaagcg ctcgaccgct ggggctacgg cctggcctcg gtccgcttca tctgcggtac
     1381 gcaggaggtc cacaaggagc tggagcggcg gatctccgga ttcctcggcc aggaggacac
     1441 catcctctac tcctcctgct tcgacgccaa cggcggtgtc ttcgagaccc tgctcggccc
     1501 ggacgacgcg gtcatctccg acgctctcaa ccacgcctcc atcatcgacg gcatccggct
     1561 ctccaaggcc cgccgcctgc gctacgccaa ccgcgacatg gccgacctgg agcgccagtt
     1621 gaaggaggcc gagggcgccc ggcgcaccct cgtcgtcacc gacggcgtct tctccatgga
     1681 cggctacctg gcgccgctgc gcgagatctg cgacctggcc gagcgctacg gcgcgatggt
     1741 gatggtcgac gactcgcacg cggtcggctt cgtcggcccc ggcgggcgcg gcacacccga
     1801 gctgcacggc gtgatggacc gcgtcgacat catcaccggc accctcggca aggcgctcgg
     1861 cggcgcctcc ggcggctatg tcgccgcccg cgccgagatc gtcgccctgc tgcgccagcg
     1921 ctcccgcccg tacctcttct ccaactcgct ggcgccggtg atcgcggcgg cctccctcaa
     1981 ggtgctcgac ctgctggagt ccgccggcga cctgcgcgag cggctgcacg ccaacaccgc
     2041 gctgttccgc tcccggatga ccgaggaggg cttcgagatc ctgcccggcg agcacgccat
     2101 cgccccggtc atgatcggcg acgcggccga ggcgggccgg atggcggagc tgctgctgga
     2161 gcgcggggtg tacgtcatcg ggttctcgta cccggtcgtc ccccaggggc aggcgcggat
     2221 ccgcgtccag ctgtccgcgg cgcactccac cgacgacgtg caccgcgcgg tggacgcctt
     2281 cgtggccgcc cgcgccgcgc tggcccgctg aagcgaccgg tccgcaaggg aacaaccggc
     2341 cgcaccctgc gagactggaa gcatgatcga agcacgccgg ctgcacatcc tccgcgcgct
     2401 ggccgaccac cgcacggtca ccgccgcggc agccgcgctc tatctcacgc cctcggcggt
     2461 ctcccagcag ttggcggccc tggagcagga gaccggccac cgccttgtgg agcgcggcgc
     2521 ccgcggtgtc cggctgaccg ccgcgggcga catcctgctg ggccacgcca acgccgtcct
     2581 ggcccagctg gagcgcgcag agtcggagct cgccgcctat gaggcagggg aggcgggcac
     2641 ggtgacggtg gcggcgttcg cgaccggcat cggcctggtg ctcgccccgg cgatcaccga
     2701 gctggcccgg tccgcgcccg gcatccgcgt ccgcgtccag gacgccgagg gcgacgccag
     2761 cctgctgatg gtgctggacc ggcaggtcga tgtcgcggtc gcggtggagt accgcggcgc
     2821 gccgggcgag gacgatccgc ggctgacccg tgtcccgctg tacgccgagc cgttcgacgc
     2881 ggtgctgtcg cccggccacc cgctggccgg cgcggagcgg gtgccgctcg ccggtctggc
     2941 caaggacccg tggatcggcc cgtactccgg caacccctgc catgacgtgg tcgtcctggc
     3001 ctgcgagtac gccggtttcg agccgcgcct ggagcattcc tcggacgact tccgggcggt
     3061 ggtggcgctc gcctccgcgg gggcaggcgt cgcgctggtg ccgcgctcgg cgctgcgcgg
     3121 gatggatctg acggatgcgg tggtccgccc ggtcgacggg gtcgccccca cccgccgggt
     3181 cttcgccgcc gtacgccgcg gcgccgagga ccacccgctg ctgcgcccgg tgctcacggc
     3241 gctgcggacg gcggcggccg cccagggctg accgggcggg gcggtcagcc ggcggcccgg
     3301 tgcggggtcc ggccggtgcg ggtccaggtt gccagttggc gggccgtcca ggtggtgatg
     3361 acccgctccg tcggcacgcc gcactcctcg gcgcgggcgc atccgtagat ctgccagtcc
     3421 agctggccgg gcgcgtgcgc gtcgctgtcg atggagaaga gggtaccggc gtcgagggcc
     3481 tgccgcagca gccggcgcgg cggatcgaac cggtccgggc ggcagttgat ctccacggcc
     3541 gtgcggtgct cggcgcacgc cgcgaagacc tccccggcgt cgaactccga ctccgggcgc
     3601 ggcttgccgg tgatctgccg cccggtgcag tggcccaaca cgtccaccag agggttgtgt
     3661 acggccgcca gcatccggcg ggtcatcggg cccttcggca tccgcagctt ggagtggacc
     3721 gaggcgacca ccacgtcgag tgcgtccagc agctccggct cctggtcgag cgagccgtcg
     3781 tcgaggatgt cgcactcgat gccggtcagc agccggaacg gcgccagttg cgcgttcacc
     3841 accgccacca gcgccagttg cagccgcagc cgctcggcgg tcagcccgcg ggccacggtg
     3901 aggcgggggg agtggtcggt cagcacgcac cagtcgtggc cgaggtcgcg ggcggccctg
     3961 gccatcgcct ccacggggct gccgccgtcc gaccagtccg agtgcagatg gcagtccccg
     4021 cgcagcgccc gcaacagcgc ccgcccctgc tccccttcga ccagcggtcc gccggcctcc
     4081 tccgccagcc gcgccaggta ggcgggctgc cgccccgcga gggcctcttc gacgacccgg
     4141 gtggttttcg ggccgagccc tttgaggcgg cccagcgtcc cgttcgccgc ccgctcgcgc
     4201 agttcctccg cgggcagttc gccgacgacc gacgccgccg tacggaacgc ctgcacccgg
     4261 taggtgaccg cgccctgccg ctccagcagg aacgcgatcc gctccagcgc cgccacggga
     4321 tccacctccc caccgtgccc cagcggggga ccggctgcca ccgcgggcgg tgccgcgaac
     4381 ttcccctatc gcagttatgc gaatatgtcc gttctctgga tagtcgatcc gtttccacgg
     4441 caatgacgta tggatcgggg gatcatttcg gtcattacca caggggaagt cgcagagaat
     4501 catgtgcggt taaagcctct gcaacatccc gggcgagtgc ccgaaacact cgccggactt
     4561 cccgtcgggg tgccgcgctg tggagtcgta aagatcggtg gccttcggaa aatctgcaca
     4621 ccgaatgcgc agtcggcaca cgcccgatgg ttttgtgcca cagccctgac ctgcggttca
     4681 gcggtttggt gcgggaatgt ccagttcgct acggagcgca acgacgggtc tacggaaaag
     4741 aaaccgcagg agatgtgaag aagctgccac caacccatgg acgcatgtac acgtccggta
     4801 attcggtggg gcgttggcgt ccagatgggc ggttgtccga ttagcagggc aacgggccta
     4861 catgctttga tccttcgcac cgcgggagtc acggggtttc ccgaattcgg gactccgact
     4921 accgttgccg cggccgggtc aaccccaccg gacggcccgt tcgcgtgctt tttcttgata
     4981 ttgatccgaa gggaaacatc atgaacaacg cccaggttca gacccaggaa atctccgacg
     5041 ccgacctgga caacgtgtcc ggtggcctcg tcggcggcgt gctgagcacc gtcaccacca
     5101 ccaccgactc cgtcccgcag gtcgcgggtg cgctcaccac cgtcaccagc ctggtccagg
     5161 gcgccagcgg cgtggacctg aacggcaccg tcggtggcct gaccgctggt ctctgagccc
     5221 ctgtgccgct gagcggcaca tccggcatcg agcggaggta acggcacgtc ggcgccccct
     5281 ccttgccgac atgccgggcc gagtgccccg gacagcccgg ttccgaccgg gccccggggc
     5341 gcctggccgt tcgtcgcccg gacataccgt cggccgtctg cgccgtgcca tgatgtcccg
     5401 taccacatcg tgcagttgag ggaagagtga cgtgcagttc cgccagcagg ccctttccaa
     5461 gctgcagtcg cccgaggagc tcgacctgcc ggtgcgcttc gcccggccgc agggctggct
     5521 cgtcctgatc gtcacggtcc tcgtcatggc cggcgccgcc gtctgggcgt tcgccggcac
     5581 cgtctcctcg accctcggcg ccccggcgat cctcacccac ggccagggca gctacgtact
     5641 ccagagcccc gtcgccggcc aggtgacctc ggtgctcgcc gaggaaggca agcaggtcgc
     5701 cgccaacgcg cccgtcgtga aggtccgtac ggcgcagggc accaccaccg tcgtccgcgc
     5761 catcgccgcc ggacggctga cgaccctgtc cgccaccatc ggctcggtcg tcacgaccgg
     5821 cgcggacctc gcctccgtcg agaagaccgc cggcgccggg gaccggctga ccgcgaccct
     5881 ctacgtcccc gccgacagcg ccgccaccct ccccctcggc gccgccgtcg acctcagcgt
     5941 ccagtccgcg ccgacccagc agtacggcgt actgcgcggc aagatcgcgg ccatcggccg
     6001 caacacccag acccgccagc ggatttccgc cttcctcggc agcgaccagc tcggccagca
     6061 gttctcccag cacggccagc cggtcgcggt cctggtgacg ctcgacaccg cgcacaccgc
     6121 ctccggctac gcctggtcca cctcggacgg cccgcccttc gcgctggact cgatgaccca
     6181 ggccaccggc gccgtccacc tggccgcgca gcacccgatc gattggctgc tcccgtgacc
     6241 gccccgaacg actccgcggc acccccgcag cacctgccgc cggccggccg cggccggcac
     6301 cgccccgagc ccgcgccggc cgcccgccgc cgcaaggggc cgccgcccac cgccaagacc
     6361 gggaaggccg ggaagtccgt ccgcacgccc accgtgctcc agatggaggc cgtggagtgc
     6421 ggcgccgcct ccctggccat ggtgctcgcc cactacgggc gccacgtccc cctggaggag
     6481 ctgcgaatag cctgcggcgt ctcccgcgac ggctcgcgcg ccagcaacct gctcaaggcc
     6541 gcccgcagtt acggcctcca ggccaagggc atgcagatgg aaccggccgc gctcgccgag
     6601 gtctcggcgc cggccatcct cttctgggag ttcaaccact acgtcgtcta cgacggcatg
     6661 ggccgccgcc tcggccgccg cggtgtgcac atcaacgacc ccgacaaggg ccgccgcttc
     6721 gtggccatgg aggacttcga caccagcttc accggtgtcg ccctggtctt cgagcccacc
     6781 gacgccttcc gcaagggggg ccgcaagccc agcgtgctca gcgccctgcc cgcccggctg
     6841 cgcggcacca ccggcaccct gctggccgcg ctgctcgcca gcctgttgct cgtcggcgtc
     6901 ggcgccgcgg tacccgcgct cagccgtacg tacatcgaca tgttcctgat cggcaaccag
     6961 acctcgctgc tggggccgct gttcgccgcg atgaccgcga tggtggcgct caccgccgtc
     7021 ctgaccggcc tgcaacaggc caacctgctg cgcggccgga tcatctcctc caccctcagc
     7081 agcgcccgct tcctgcgcca cctcctgcgg ctgccggtca ccttcttcgc ccagcgctcg
     7141 cccgccgatc tcgtccaacg gctccagtcc aacgacgcgg tggccgagac cctcgcccgg
     7201 gacctcgccg cggcagccgt ggacggcgtc gtcgtggtcc tctacgcgct gctgctgtgg
     7261 acgtacgacc cccaactcac cgtcatcggc gtactgatcg cgctgctcaa cgtcgtcgcg
     7321 atgcggatcg tcgtccggct gcgcgccact cacgtccaga agctgcgcgc cgacaccgcc
     7381 cggctgacca acacctcgta caccggcctc cagctcatcg agaccatgaa ggccaccggc
     7441 ggcgagagcg gctacttccg gcgctgggcc ggccagcacg ccagcaccct ggaggaacag
     7501 cagcggctcg gcgttcccag cgcctggctg gcggtcgtcg cccccaccct cgccaccctc
     7561 aacagcgccc tgatcctgtg gatcggcggg ctgcgcgcgg tcgagggaca tatctccatc
     7621 ggcctgctgg tcgccttcca ggccctggtg acgcgcttca ccgcgccggt cacccggctc
     7681 aacggcgtcg ccggccgcat acaggacttc gccgccgacg tcgcccgcct caaggacgtc
     7741 gagagcttcc ccgccgacac cctgtactcc cgcccggagg cggacgccgg cacccgccgc
     7801 ctcaaaggcc atgtgacgct ggaggacatc accttcggct acagcccgct cgacaaaccg
     7861 ctgctcagcg gcttctccct ggccgtcggc cccggccgcc aggtcgccct ggtcggcggc
     7921 tccggcagcg gcaagtccac cgtctcccgg ctgatctccg gcctctacgc cccctgggaa
     7981 ggcaccatcc gcatcgacgg acagcgcctg gaggacatcc cccgcagcgt gctggccgcc
     8041 tcggtctcct tcgtcgacca ggacatcttc ctcttcgagg gcaccgtccg ggacaacgtc
     8101 gccctctggg acccgtcgat cccggacgac gcggtcatcg cagccctcca ggacgccgcc
     8161 ctctacgacg acgtcatcgc ccgccgcccc gacggcatcc acagccgcgt cgaacaggac
     8221 ggccgcaact tctccggcgg ccagcggcag cgcctggaaa tcgcccgcgc actggtacgg
     8281 cgccccagca tcctggtcct cgacgaggtc accagcgccc tggacgccga gaccgaggcg
     8341 gtcatcatgg acaacctgcg gcggcgcggc tgcgcctgcg tcgtcatcgc ccaccggctg
     8401 agcaccgtcc gcgacagcga cgagatcgtg gtcctcgacc acggcgtggt cgtcgaacgc
     8461 ggccggcacc aggacctggt cgccgccgga ggcgcgtacg ccgagctggt caaggagcac
     8521 tgacgtggca tccgtacacc cgtccccggg cacgggaccc ggcgcgggat ccaccggcgg
     8581 accgggcgag agcgacgcgg tgacgtacgc cctgggcggc ctcggcaccc ccgtcgactg
     8641 caccgggctg cgcaacgtcc ccctggaagg accgcacgtc ctgtggctgg tcaccggcgg
     8701 cttcctggac ctcttcgccg tcgacgccgc ccaggagggc cactggcact tcctcggccg
     8761 cctcgaagcc ggcgccctgc tgctcggccc cgtcgaaggc ccccagcaca ccctcttcgg
     8821 ccgcccctcc caggactgcg ggctgcgccg gatcgcgctg cgcgaactgc cccgccccga
     8881 ctacgccggc tacggcgagc agggcgagta cgcgtacggc tacgacgaca cctacggcgg
     8941 gcaggcaccc tccgcgctgg agcacgcctt cgcgctcggc accgcccgca gcctgggcgt
     9001 gctgttcgag gcaccgctgg acggcaggcc caccgacgag gcggtggccg acgacgacat
     9061 cctctggatg ccggtaccgc ccggcagcgt ccagtacggc gcctcctaca gcgccgaggc
     9121 cgccgccgac ctgctcgtcg accccgcgct gtggcagcgc atggtcaacc agcagtaccg
     9181 gttgctttcc gccgtcgacc gctggatcga gcagctggag cgcgcccacg aggaccgcac
     9241 cgccgccggc atcaaggcgg gcgaggccgt ccgcgaacgc gccgaccagg cgctgctcgc
     9301 ctccatcggc aaggaccgct cgggacgctc cgccgcgggc cgcgaccggg ccggcgacga
     9361 cgccaccttc gcggtctgcc gccaggtcgc cgcggcggcc ggcatcaccc tgaccgagcc
     9421 ccccaagggc gccgcggcca acgaccggat cagccccgtc gaacgcatcg cggtcagctc
     9481 ccggatccgc acccgcgccg tacgcctcga cgggcgctgg tggcgcaccg acgccggccc
     9541 cctggtcggc atgcgcgcca agtccggcgc gcccgtggcg ctgctgtggc gccgcggggg
     9601 ctacgaggcg gtcaaccccg cctccggcct gcggatccgc atcggcaagg acaacgccga
     9661 cgagttcgag ccgcgcgcgg tgatgttcta ccggccgctg ccggaccgcc cgatgagcat
     9721 gtggcggctg atgcgcttca gcctgcgcgg cacccgcatg gacgtgcgta atctcgctct
     9781 cggcggcctg gtgacggtcg gcctcggcgc gctcgtcccg atcgccaccg gcagggtcct
     9841 cggcgtctac gtaccggccg ccgagcgcag cctgatcgtc caggtcgccc tcgccgtcat
     9901 cgtctccggc gtggtgtccg ccgccttcat gctcctccag aacctcaccg tgctgcgcat
     9961 ggagggccgc atcgagagca ccctgcagcc cgccgtctgg gaccggctgc tgcgccttcc
    10021 gacgaagttc ttcgccgagc gctccaccgg cgaactggcc agcgccgcga tgggcatcag
    10081 cgccatccgc cgggtgcttg ccggcgtcgg gccggtcgcg gtgcaggcca ccacggtcgg
    10141 cgccatgaac ctcgtcctgc tgctcctcta cagcgtcccg ctcgccctcg ccgcgatcgc
    10201 catgctgctg gtgatc
//
