LOCUS       NZ_CP047146            10417 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. GS7 chromosome, complete genome.
ACCESSION   NZ_CP047146
VERSION     NZ_CP047146.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GS7
  ORGANISM  Streptomyces sp. GS7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9067183)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9067183)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-NOV-2019
            Assembly Method                   :: SPAdes v. Nov-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:53:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,019
            CDSs (total)                      :: 7,925
            Genes (coding)                    :: 7,616
            CDSs (with protein)               :: 7,616
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 107 of 309
            Pseudo Genes (incomplete)         :: 258 of 309
            Pseudo Genes (internal stop)      :: 29 of 309
            Pseudo Genes (multiple problems)  :: 79 of 309
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:40:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2449337
            Orig. end                         :: 2459754
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047146.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="5"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5417)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            535..1083
                     /locus_tag="GR130_RS10535"
                     /old_locus_tag="GR130_10535"
     CDS             535..1083
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536869.1"
                     /locus_tag="GR130_RS10535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_10535"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_159504469.1"
                     /transl_table=11
                     /translation="MHPETRDPALQVRSATPADLEPIAALHTAARAAYLRGRVPDAPFD
                     TPAEHARWRDAWARVLHQEGTPSLCAIRGGTVVGAASYRRRTDPRGTVALHQLHVDPAH
                     WGTGVGRALHAACLDAWRTAGFSRAVLDVLWHNHRARAFYARLGWGPDPDRRPAPDASH
                     LTLTLALPGRDGRPVPAGP"
     gene            complement(1025..1720)
                     /locus_tag="GR130_RS10540"
                     /old_locus_tag="GR130_10540"
     CDS             complement(1025..1720)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985685.1"
                     /locus_tag="GR130_RS10540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_10540"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_159504470.1"
                     /transl_table=11
                     /translation="MTTPSPQGPDRFWETTGAAKTFTHPLDPALLAEFVPPAARVLDYG
                     CGYGRLTAELAGLGYGAVCGVDPSAALIARGRREHPGLELLHRPGLPLPFGDASFDAAL
                     LFAVLTCVPEDAEQRAIAGELGRLVRPGGVLYLSDVPLQADAHNQERYGRFAERYGTFG
                     VFATDEGGVFRHHPPERLRELVRSAGFSVVRERRETVRTLSGRTAERLQIMARREPAGR
                     PGRAGRASG"
     gene            1825..2529
                     /locus_tag="GR130_RS10545"
                     /old_locus_tag="GR130_10545"
     CDS             1825..2529
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091831.1"
                     /locus_tag="GR130_RS10545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_10545"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_159504471.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFASGLAAFAHRDDVEVVHRSFELDP
                     NAPAPTDIPVLDMLARKYRVTRTEAEAMEARVAEAASGEGLDYRSDRIHGNTFDLHRLL
                     HLAKDHGVQDALLSALYDANFAQARPLGDPAELTRISVAAGLPADEVARVLADPDAYAE
                     AVRADERAAAELGATGVPFFVIDRRYGVSGAQPAEVFRQALERAHADGAVRILPAGDAE
                     VCGDEGCAVPER"
     gene            2602..3663
                     /locus_tag="GR130_RS10550"
                     /old_locus_tag="GR130_10550"
     CDS             2602..3663
                     /NRPS_PKS="Domain: Aminotran_5 (16-346). E-value: 1.9e-26.
                     Score: 84.8. Matches aSDomain:
                     nrpspksdomains_GR130_RS10550_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536872.1"
                     /locus_tag="GR130_RS10550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_10550"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_159504472.1"
                     /sec_met_domain="Aminotran_5 (E-value: 6.8e-17, bitscore:
                     60.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRYEALGGDEFAPETVYLNSASSGLLPARSAAALREALTESASY
                     GTMGRDYFAPATAARRAFGRLTGVPEERVALGSSVAVRSAFVAASLPAGAEVLVAEGDF
                     SSLVNPLAAHPGCTVRAVPLEALAEEVRPRTALVAVSAVQALDGRIADLAAVREAARAH
                     GARTYVDITQSAGWLPLDIAGFDFVVCGAFKWLLCPRGTTFLYLGGDAADWPPAVHAGW
                     VAGEDVGESNYGVIGRPAATARRFDEPYAYYSYVAAEHSLGLMAEVGVAAVHAHNTALA
                     DRYRAGLAEAGFPSRPAPGSAIVSTPGLAGAEPRLAAAGVRIAVRGGLLRASFHLYNSA
                     RDVEKVLALLREA"
     misc_feature    2603..2619
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.0"
                     /tool="antismash"
     aSDomain        2650..3639
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR130_RS10550_Aminotran_5.1"
                     /evalue="1.90E-26"
                     /label="GR130_RS10550_Aminotran_5.1"
                     /locus_tag="GR130_RS10550"
                     /protein_end="346"
                     /protein_start="16"
                     /score="84.8"
                     /tool="antismash"
                     /translation="VYLNSASSGLLPARSAAALREALTESASYGTMGRDYFAPATAARR
                     AFGRLTGVPEERVALGSSVAVRSAFVAASLPAGAEVLVAEGDFSSLVNPLAAHPGCTVR
                     AVPLEALAEEVRPRTALVAVSAVQALDGRIADLAAVREAARAHGARTYVDITQSAGWLP
                     LDIAGFDFVVCGAFKWLLCPRGTTFLYLGGDAADWPPAVHAGWVAGEDVGESNYGVIGR
                     PAATARRFDEPYAYYSYVAAEHSLGLMAEVGVAAVHAHNTALADRYRAGLAEAGFPSRP
                     APGSAIVSTPGLAGAEPRLAAAGVRIAVRGGLLRASFHLYNSARDVEKV"
     gene            complement(3991..4893)
                     /gene="thpD"
                     /locus_tag="GR130_RS10555"
                     /old_locus_tag="GR130_10555"
     CDS             complement(3991..4893)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="GR130_RS10555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_10555"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_159504473.1"
                     /sec_met_domain="PhyH (E-value: 2.2e-37, bitscore: 128.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTTEVITPRKDPVVWSQPGTAGPFAPSELS
                     DFERDGFFAIGELLTAEEVAVYRAELDRLVLDPAMRADPRSIVEPKSQSVRSVFEVHRI
                     SEVFARLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLA
                     NMRTVSVSIALTENYDTNGGLMIMPGSHRHFVGCEGETPKDNYKRSLQMQDAGIPSDEV
                     LTKMADRHGIRLFTGRAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVDNAAVEPFA
                     APVRRPEYIGARAPFVPVK"
     gene            complement(5001..5417)
                     /locus_tag="GR130_RS10560"
                     /old_locus_tag="GR130_10560"
     CDS             complement(5001..5417)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606340.1"
                     /locus_tag="GR130_RS10560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_10560"
                     /product="ectoine synthase"
                     /protein_id="WP_159504474.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.1e-53, bitscore:
                     176.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFQDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVVCVEGEAELTNDETGEKHTITPGVMYLLDGHEKHTMRIKKDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPDPEPEAG"
     gene            complement(5464..6732)
                     /gene="ectB"
                     /locus_tag="GR130_RS10565"
                     /old_locus_tag="GR130_10565"
     CDS             complement(5464..6732)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 4.3e-73.
                     Score: 238.2. Matches aSDomain:
                     nrpspksdomains_GR130_RS10565_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="GR130_RS10565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_10565"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_159504475.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.2e-76, bitscore:
                     254.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAQGSRMFDEDGHAY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNIVLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDHYLDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALADLCERQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTLGRGEIVESHLK
                     AIVEAHPDTFVEYRGRGLVWGMECADKSLAGKIAKRAFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRILARAVRDCA"
     aSDomain        complement(5638..6648)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR130_RS10565_Aminotran_3.1"
                     /evalue="4.30E-73"
                     /label="GR130_RS10565_Aminotran_3.1"
                     /locus_tag="GR130_RS10565"
                     /protein_end="365"
                     /protein_start="28"
                     /score="238.2"
                     /tool="antismash"
                     /translation="FDRAQGSRMFDEDGHAYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNIVLRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDHYLDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALADLCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDTYWADGQMEKQTLGRGEIVESHLKAIVEAHPDTFVEYRGRGLVWGMECA"
     gene            complement(6883..7449)
                     /gene="ectA"
                     /locus_tag="GR130_RS10570"
                     /old_locus_tag="GR130_10570"
     CDS             complement(6883..7449)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156969.1"
                     /locus_tag="GR130_RS10570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_10570"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_159504476.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSEIREIPEGFKLDTPRVEDGAAIWRIARDSKAL
                     DLNSSYSYLLWCRDFAATSVVARDAEGEPAAFVTGYVRPERPDTLVVWQVAVDGAHRGR
                     GLAAALLDGLTTRVMDEQGISLVETTITPDNAASNRLFASFAERHSVPVKREVLFDAGL
                     FPEQGHEPEVLNLIGPFRPPSRPGR"
     misc_feature    complement(7449..7463)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.04"
                     /tool="antismash"
     misc_feature    7605..7620
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    7615..7630
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7615..7632)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.3"
                     /tool="antismash"
     gene            7945..9036
                     /locus_tag="GR130_RS10575"
                     /old_locus_tag="GR130_10575"
     CDS             7945..9036
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576771.1"
                     /locus_tag="GR130_RS10575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_10575"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_201305160.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 5e-19, bitscore:
                     67.6, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDGAVLHLKGRVLVGPDDVRDELWVVDGRVTFERPAMARDAMTV
                     AGWVLPGLVDAHCHVGLDAHGPVDAATSEKQALTDRDAGTLLIRDAGSPSDTRWIDDRA
                     DLPRIIRAGRHIARTRRYIRNYAHEIEPEDLVAYVAQEARRGDGWVKLVGDWIDRSTGD
                     LAACWPAGAVEAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCVEHATGLTEDTIPLF
                     AERGVAIVPTLVNIATFPQLAAGGEAKFPRWADHMRRLHARRYDTVRAAHDAGVPVFAG
                     TDAGGSLPHGLVADEVAELMKAGLSAVAALSATTWGAREWLGRPGLTEGAPADLVVYEA
                     DPRADVRVLAAPRLVVLRGRAIG"
     gene            9322..10272
                     /locus_tag="GR130_RS10580"
                     /old_locus_tag="GR130_10580"
     CDS             9322..10272
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536882.1"
                     /locus_tag="GR130_RS10580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR130_10580"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_159504477.1"
                     /transl_table=11
                     /translation="MLTSSAGSSGVSRRLGAALAAAALTAGPVVALGAAPAAYAAGNDG
                     RGDGTSGAAVLRTGLDVGLLDKSLRVPLRATLNDVHAPASAGRTALTVTLDGIDQGRPF
                     SVLRADVATARATADAEGAKGYANLVHAAVHLPGLPALPLIEVQQVTSTAECRAGQHPR
                     ATSQVLGSVQVLGKRVTLGASGTTAVTVPGVGDVRLDLSHKAVTSTSAAATALQLRVSV
                     NPLKLNVAEVHGQVTLAQATCTTPHGTAPGTPEKPGEPQGGAGGTRPATQNLAETGGSS
                     ATPYVAGGAALLVAAGAGSLIHARRRRAAAPGGRG"
     misc_feature    9325..9327
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 tggcccccgg cgacggggac ggcgaacaca ccgtcggcgc cggcttcctc accgtcgcgg
       61 ccgcccggct cgccgacggg ggcccgcacg acctgctgct ggaggagtgc ttcggcccgg
      121 tcaccgtcgt cgcccgctac gccgacgacg ccgagatcgg cgccgtcctc gcccggctcc
      181 ccggcaacct caccgccacc ctccagctcg gcgacgcgga gagcgccggc accgacccgg
      241 cgggccgcgg cgcccgcctg ctggccgagg tcaccccact cgccggccgc gtcgtcgtca
      301 acggctggcc caccagcgtc gcggtcgccc ccgcccagca ccacggcggc ccgtacccgg
      361 ccgccacctc cacttccacc tccgtcggcg cgaccgccgt cgaacgctgg ctccgcccgg
      421 tcgcctacca ggacaccccg ccggcgctgc tcccgccgga gctgcgggac ggcaacccgc
      481 tggggctgcc gcgccggatc gacggccggg cagagggtgc gtagggcccc gcaggtgcac
      541 cccgagaccc gcgaccccgc cctccaggtc cgctccgcca cccccgccga cctggagccc
      601 atcgccgccc tgcacaccgc cgcccgcgcc gcgtacctgc gcggccgggt gccggacgcg
      661 cccttcgaca cccccgccga gcacgcccgg tggcgggacg cctgggcccg ggtgctgcac
      721 caggaaggga ccccgtcgct gtgcgcgata cgcggcggca ccgtcgtcgg cgccgcctcg
      781 taccgccgcc gcaccgaccc ccgcggcacc gtcgcactgc accagctgca cgtcgacccc
      841 gcccactggg gcaccggcgt gggccgcgca ctgcacgccg cctgcctgga cgcctggcgc
      901 accgccggat tctcccgggc cgtcctcgac gtgctctggc acaaccaccg cgcccgcgcc
      961 ttctacgccc gcctcggctg gggccccgac ccggaccgcc gcccggcccc cgacgccagc
     1021 cacctcaccc tgacgctcgc cctgcccggc cgggacggcc ggccggttcc cgccgggcca
     1081 tgatctgcag ccgctcggcc gtccgtccgc tcagcgtgcg gaccgtctcg cgccgctccc
     1141 gcaccaccga gaaccccgcc gaccgcacca gctcccgcag ccgctccggc ggatggtgcc
     1201 ggaagacccc gccctcgtcg gtcgcgaaca ccccgaacgt cccgtaccgc tccgcgaagc
     1261 gcccgtagcg ctcctggttg tgcgcgtcgg cctggagcgg cacatcgctc aggtacagca
     1321 ccccgccggg gcgcaccagc cgccccagct ccccggcgat cgcccgctgc tccgcgtcct
     1381 cggggacaca ggtcagcacc gcgaacagca gcgccgcgtc gaagctcgcg tccccgaacg
     1441 gcagcggcag accgggccgg tgcagcagct ccagccccgg gtgctcgcgc cgcccgcgcg
     1501 cgatcagcgc cgcggacggg tcgaccccgc acaccgcccc gtaccccagc ccggccagct
     1561 cggcggtgag ccgcccgtag ccgcagccgt agtccagcac ccgcgcggcg ggcggcacga
     1621 actccgccag cagcgccggg tcgagcgggt gggtgaaggt cttggcggcc ccggtcgtct
     1681 cccagaaccg gtccggcccc tgcggcgatg gcgtggtcat accgggacgg tacccgcgga
     1741 atactccgca atcctgcgct gttggaccag gtcacacccg cacgcccgct cccaccgagc
     1801 acgacgagga aagccagcca ctccatgcgc gtcgaaatct ggtccgacat cgcctgcccc
     1861 tggtgctaca tcggcaaggc ccgcttcgcg tcggggctcg ccgccttcgc gcaccgcgac
     1921 gacgtcgagg tcgtccaccg ctccttcgag ctggacccga acgccccggc gcccaccgac
     1981 atccccgtcc tcgacatgct cgcgcggaag taccgcgtca cccgcaccga ggccgaggcg
     2041 atggaggccc gggtcgccga ggcggcgtcc ggcgagggcc tggactaccg ctccgaccgc
     2101 atccacggca acaccttcga cctgcaccgt ctcctccacc tagccaagga ccacggcgtc
     2161 caggacgccc tgctgtccgc cctctacgac gccaacttcg cccaggcccg gccgctcggc
     2221 gaccctgccg agctgacccg gatatccgtg gccgccggcc ttcccgccga cgaggtcgcc
     2281 cgcgtgctgg ccgacccgga cgcctacgcc gaggcggtgc gggccgacga gcgcgccgcc
     2341 gccgagctcg gtgccaccgg cgtgcccttc ttcgtcatcg accgccgcta cggcgtctcc
     2401 ggggcccagc ccgccgaggt cttccgccag gccctggagc gcgcccacgc cgacggcgcc
     2461 gtgcggatcc tgccggccgg cgacgcggag gtttgcggcg acgagggctg cgccgtcccg
     2521 gaacggtagg gccgcaacgg gacggtccgg cgcggctccc gtgagatgga cgccgcgctg
     2581 ttcgccgagc acagtggggc catgctgcga tacgaggcac tgggcgggga cgaattcgcc
     2641 cccgagacgg tctacctgaa ctcggcgtcc agcgggctgc tgcccgcgcg gagcgccgcc
     2701 gcgctgcggg aggcgctgac ggagtcggcg tcgtacggca cgatggggcg cgactacttc
     2761 gctccggcca cggccgcgcg gcgggcgttc gggcggctga cgggggtgcc cgaggagcgg
     2821 gtggccctgg gcagttcggt cgcggtgcgg tcggcgttcg tggcggcctc gctgcccgcg
     2881 ggcgccgaag tgctggtcgc cgagggggac ttcagctcgc tggtgaaccc gctggcggcg
     2941 caccccggct gcacggtccg cgcggtaccg ctggaggcgc tcgccgagga ggtacggccc
     3001 cggacggcac tggtcgcggt gagcgcggta caggcgctgg acggccggat cgccgacctg
     3061 gcggccgtac gggaggcggc ccgggcgcat ggggcgcgca cgtacgtcga catcacacag
     3121 tccgccggat ggctgcccct ggacatcgcc ggcttcgact tcgtggtgtg cggggccttc
     3181 aagtggctgc tgtgcccgcg cggtacgacg ttcctgtacc tcggcggcga cgcggccgac
     3241 tggcccccgg cggtgcacgc cgggtgggtg gccggggagg acgtcggcga gtcgaactac
     3301 ggggtgatcg gccggccggc ggcgaccgcc cggcggttcg acgagccgta cgcgtactac
     3361 tcgtacgtgg ccgccgagca ctccctcggc ctgatggcgg aggtgggggt ggccgcggtg
     3421 cacgcgcaca acaccgcgct ggccgaccgg taccgcgccg ggctggccga ggccggcttc
     3481 ccctcgcggc cggcgcccgg ctccgcgatc gtctccaccc cggggctcgc cggcgcggag
     3541 ccgcggctgg ccgcggccgg ggtgcggatc gcggtgcgcg gcgggctgct gcgcgcctcg
     3601 ttccacctct acaactcggc gcgggacgtg gagaaggtgc tggcgctgct gcgtgaggcg
     3661 tgaggcgtga ggcgggcggg gagccgggga gcgggggagc cggggaacac cgggaagacg
     3721 ccgcggtccc gggacgcaga ggtgagggcc aacatctcgt ctctgtgggc gagttaccgt
     3781 ccgcgcgccg ggacggcggc cccgtcgacc gcagttgaga ggccgtcagt gaccaccgcg
     3841 tgcgcggtgg accgaccgtc cggcgggtcg gcaccgccga cgcccggtcg gaacgcgccg
     3901 tggccgccgc acggtcgatg ccgtgcggcg gccaggagcg ctccgcgccg tccccggtac
     3961 gcggtcgtgc gccagcgcac cggcgtgccc ctacttgacg ggaacgaacg gggcccgcgc
     4021 cccgatgtac tccgggcgcc ggaccggcgc cgcgaacggc tccacggcgg cgttgtccac
     4081 gctgttgaag acgatgaaga cgttgctgcg cgggtacggc gtgatgttgt cgccggagcc
     4141 gtgcatggcg ttgcagtcga accacgtcgc cgagccggcc cggccggtga acagccggat
     4201 gccgtgccgg tccgccatct tcgtgagcac ctcgtccgac gggatgcccg cgtcctgcat
     4261 ctggagcgac cgcttgtagt tgtccttcgg cgtctcgccc tcgcagccca cgaagtgccg
     4321 gtgcgaaccg ggcatgatca tcaggccgcc gttggtgtcg tagttctcgg tcagcgcgat
     4381 cgagaccgac acggtgcgca tgttcgccag accgtcctcg gcgtgccagg tctcgaagtc
     4441 ggagtgccag tagaagccgg aggcaccgaa gccgggcttc acgttgatcc gcgactggtg
     4501 gacgtagacg tccgaaccga ggatctggcg ggcccggccg accacccgcg ggtcgcggac
     4561 cagccgggcg aacacctcgc tgatcctgtg cacttcgaag acggaccgga cgctctgcga
     4621 cttcggctcg acgatggagc gcgggtcggc gcgcatcgcc gggtcgagca ccagccggtc
     4681 cagctcggcg cggtacaccg cgacttcctc tgccgtgagc agctccccga tggcgaaaaa
     4741 gccgtcgcgt tcaaagtcgc tcagctcgga cggcgcgaac gggccggccg ttccgggctg
     4801 cgaccacacc accgggtcct tccgcggggt gatgacctct gtggtcccac gggtcgggta
     4861 caggtcggcg gtgcgctcgg gtgcggtggt catggtggtg ccttcctctc ctctcggacg
     4921 gaacgtggtt ctcacgcgcg cggtgcgttg ccctcctcac cgctgccgga cggggcgggg
     4981 aggggggccg gtggccggtc tcagcccgcc tcgggctccg ggtcgggttc ggtgagaagg
     5041 gggtagaccc cgttctcgtc gtggtcctcg cggccggtga ccggcgggtt gaagacgcac
     5101 aggcagcgga agtccttctt gatccgcatg gtgtgcttct cgtgcccgtc gagcaggtac
     5161 atcacaccgg gcgtgatggt gtgcttctcg ccggtctcgt cgttggtcag ctcggcctcg
     5221 ccctcgacgc acacgacggc ctcgatgtgg ttcgcgtacc acatcgaggt ctcggtgccg
     5281 gcgtagagga cggtctcgtg cagcgagaag ccgacccgct ccttggcgag cacgatgcgc
     5341 ttgctctccc aggtgccgga cttggccttg acgtggcggt cggtgccttc gatgtcctgg
     5401 aacgatcgga cgatcacggt gagttggtgc ctttctgtgg aacggtcggg agctctcggt
     5461 ggatcaggcg cagtcgcgga ccgcgcgggc gaggatccgc agaccctcgt ccagctcctc
     5521 gggggtggtc gtcagcgcgg gcagcagctt gacgacctcg ctctccgggc cggaggtctc
     5581 gatgagcaga cccagctcga aggcgcgctt ggcgatcttg ccggcgaggc tcttgtcggc
     5641 gcactccatg ccccacacca gaccgcggcc gcggtactcg acgaaggtgt ccgggtgcgc
     5701 ctcgacgatg gccttgaggt gcgactcgac gatctcaccg cggccgaggg tctgcttctc
     5761 catctgaccg tcggcccagt acgtgtcgag cgctgcggcg gcggtgacga aggccgggtt
     5821 gttgccgcgg aaggtgccgt tgtgctcgcc cggttcccag atgtccagct cgggcttgaa
     5881 cagcgtcagc gcgagcggga gcccgtagcc gctgatggac ttcgagacgg tgacgatgtc
     5941 cggcacgatg cccgcctcct cgaaggagaa gaaggcaccc gtacggccgc agcccatctg
     6001 gatgtcgtcg acgatcagca gcatgtcctg gcgctcgcac aggtcggcga gggcgcgcag
     6061 ccactcggcg cgggccacgt tgatgccgcc ctcgccctgc acggtctcca cgatcacggc
     6121 cgcgggcttg ttgaggccgg agccctggtc ctccagcagc cgctcgaacc agaggaagtc
     6181 ggggacctgg ccgtcgaggt agtggtcgaa cggcatcggg gtgccgtgca ccagcgggat
     6241 gccggcgccg gcccgcttga aggcgttgcc ggtgaccgcg agggagccca gcgacatgcc
     6301 gtggaaggcg ttggtgaagg acacgatcga ctcgcggccc ttgaccttgc gggccagttt
     6361 cagcgcggcc tcgacggcgt tggtgcccgt cgggcccggg aacatgacct tgtagggcag
     6421 gtcgcgcggg cgcaggacga tgttctggaa cgactccagg aaggcccgtt tggccgtggt
     6481 ggacatgtcc aggccgtggg tgacgccgtc ccgctcgatg tagtcgatca gggcgcgttt
     6541 gaggaccggg ttgttgtgcc cgtagttgag cgacccggca ccggcgaaga agtcgaggta
     6601 cgcatggccg tcctcgtcga acatgcggct gccctgggcg cggtcgaaga cggtgggcca
     6661 gccacggcag tagctgcgca cctccgactc cacggtctcg aagacgctca ggtcgggctg
     6721 ggtgatggtc acggcaggct cctgggagaa gaaagcggag ggggagaagt ccgggggtgg
     6781 atcgggtggg gcggtgcggc tcccgtgtgg ttcacgggtt cgggcggtgg tcccgcacgg
     6841 ccgctgcggc atggtcggtg ccgcgcggcc ctggggccgt cgtcagcgtc cggggcggga
     6901 cggtggacgg aacgggccta tgaggttcag gacctccggc tcgtggccct gctccgggaa
     6961 cagccccgcg tcgaagagca cctcgcgctt gaccggcacc gagtgccgct cggcgaagga
     7021 cgcgaacagc cggttggacg cggcgttgtc aggggtgatg gtcgtctcga cgagggatat
     7081 gccctgttcg tccatgacgc gggtggtcag cccgtccagg agcgcggcgg ccagcccccg
     7141 tccgcggtgc gcgccgtcga cggcgacctg ccagacgacg agcgtgtccg ggcgctcggg
     7201 gcggacgtag ccggtgacga aggcggccgg ctcgccctcc gcgtcacgtg cgacgacgga
     7261 ggtggcggcg aagtcgcgac accacaggag gtagctgtag gaggagttga ggtccagtgc
     7321 cttggagtcg cgggcgatgc gccagatcgc ggctccgtcc tccacgcgcg gggtgtcgag
     7381 cttgaagccc tccggaattt ccctgatttc gcttcgggca cctgcatggt ctgcttgtgc
     7441 ggcggtcatg gaaattaaat ttaccgaggg aaaaaggaaa atgcatcgcg ggcaggggtt
     7501 acatgggact cctctttgtg ttatcacgcg ggggcgcgcg agcgcgcggt gcgtcggcaa
     7561 gatgtctcga tttgacccga aagttcgggg cgaaacggac acgctgtgta gtcggtcaca
     7621 gctccgtaac ggcgatgaga tgcgtccgaa ttacgggact tgacgctagc gaaatcttgg
     7681 cgtttgaggt gcggaaaagc gggcagaaga atacgggaag ctacgcttct gcgaaatggt
     7741 aattcgccgg tggaagatgc ccggagcagt tcaccgcaag gtacgccgga agtcttgccg
     7801 gcgacatatg agaagccccg tgggaaggcg cgtgagaagc cgcgggtgat tatggggcgg
     7861 ccggtcgaat acgacccccg ggtatgctcc ggtccgccat ggcccacggc tcggccgcac
     7921 gcataaagtg cctgcatgac gtccatgagc gatggtgcgg tgctgcacct caaggggagg
     7981 gtcctcgtcg ggccggacga cgtacgcgac gagctgtggg tggtcgacgg ccgggtgacc
     8041 ttcgagcggc cggcgatggc acgcgatgcc atgaccgtcg cgggctgggt gctgccgggc
     8101 ctggtcgacg cccactgcca cgtcggactg gacgcgcacg gcccggtgga cgccgccacc
     8161 agcgagaagc aggcgctcac cgaccgtgac gccggcaccc tcctgatccg ggacgccgga
     8221 tccccctccg acacccgctg gatcgacgac cgcgccgacc tgccgcggat catccgcgcc
     8281 ggccgccaca tcgcgcgcac caggcgctac atccgcaact acgcgcacga gatcgagccc
     8341 gaggacctgg tcgcctacgt cgcccaggag gcccggcgcg gcgacggctg ggtcaagctc
     8401 gtcggcgact ggatcgaccg ctccaccggc gacctggcgg cctgctggcc cgccggcgcc
     8461 gtcgaggccg ccatcgccga ggcgcaccgg ctcggcgccc gggtcaccgc ccactgcttc
     8521 gccgaggagt ccctcgcccc gctcgtcgag gccggcatcg actgcgtcga gcacgccacc
     8581 ggcctcaccg aggacaccat cccgctcttc gccgaacgcg gtgtggcgat cgtcccgacc
     8641 ctggtcaaca tcgcgacctt cccgcagctc gcggccggcg gcgaggcgaa gttcccgcgc
     8701 tgggcggacc atatgcgccg gctgcacgcc cgccgctacg acaccgtgcg cgcggcccac
     8761 gacgccgggg tgccggtctt cgccggtacc gacgcgggcg gctcgctgcc gcacggcctg
     8821 gtcgccgacg aggtcgccga gctcatgaag gccgggctgt ccgccgtcgc cgccctctcc
     8881 gcgacgacct ggggcgcccg cgagtggctg ggccgcccgg ggctcaccga gggcgccccg
     8941 gccgacctcg tcgtctacga ggccgacccc cgggccgacg tccgggtgct ggcggcgccg
     9001 cgcctggtgg tcctgcgggg gcgggccatc ggctgacggg cggtgcccgg tgccgcccgc
     9061 ggcaccgggg cggcaccggg cggccgtgcg ccggccgcgt cgcggagccg gccgccgcca
     9121 tgccggcctc tggcctccgt ccgatgcgtc gaggcgctcg aataagggcg cgaaaaccac
     9181 cccttggggt gaactgcctc tccatttcct cctgttcacc ctccgtacgt acacgatgtg
     9241 cgggtcgagg tcgccggccc gcgcgatgtc ccccattggc gcggtcggcg gctccatctc
     9301 tctcgtgggg gttccacaca cgtgttaacc agttccgccg gctcctccgg cgtgtcgcgg
     9361 cggctcggcg cggccctggc cgccgccgcg ctgacggccg gtcccgtcgt cgccctcggc
     9421 gccgcacccg ccgcgtacgc cgccggcaac gacggccgtg gcgacggcac ttcgggtgcg
     9481 gccgtgctgc gcaccggcct ggacgtcggg ctgctcgaca agtcgctccg cgtcccgctg
     9541 cgggccaccc tgaacgacgt gcacgcaccg gcctcggccg gcaggaccgc gctcaccgtc
     9601 accctggacg ggatcgacca gggccggccg ttcagcgtgc tgcgggccga tgtcgccacc
     9661 gcgcgggcca ccgccgacgc cgagggcgcg aaaggctacg ccaacctcgt gcacgccgcg
     9721 gtgcacctgc cgggcctccc cgcgctgccg ctgatcgagg tccagcaggt gacctccacg
     9781 gcggagtgcc gggccgggca gcacccgcgg gccacgtcgc aagtgctggg cagcgtccag
     9841 gtgctgggca agcgggtcac gctcggcgcg agcgggacga cggcggtcac cgtgccgggg
     9901 gtgggcgacg tacggctcga tctgtcgcac aaggccgtga cgtcgacgag cgccgcggcg
     9961 accgcgctcc aactccgggt ctccgtcaac ccgttgaagc tcaatgtggc cgaggtgcac
    10021 ggccaggtga cgctggcgca ggccacctgc accaccccgc acggtacggc gcccggcaca
    10081 ccggagaagc ccggggaacc ccagggcggc gccggcggca cccgcccggc cacgcagaac
    10141 ctcgccgaga ccggcggcag ttcggccacc ccgtatgtcg ccgggggcgc ggccctgctg
    10201 gtcgccgccg gcgccggatc gctgatccac gcccgtcgcc gccgcgccgc cgcaccgggc
    10261 gggcgcggct gacaggaccc gtacgccgtg ccggtgctgt gggggctccg gtgcggggta
    10321 cggaccggag gcgcgccggg tgggcccgcg cggcgcgcct cccctcgccg gggtcctggt
    10381 cggagcgtgg tccggccggg gccccgggga gaccctc
//
