LOCUS       NZ_CP047147            10216 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. GF20 chromosome, complete genome.
ACCESSION   NZ_CP047147
VERSION     NZ_CP047147.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GF20
  ORGANISM  Streptomyces sp. GF20
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7311137)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7311137)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: SPAdes v. Nov-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:38:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,372
            CDSs (total)                      :: 6,281
            Genes (coding)                    :: 6,197
            CDSs (with protein)               :: 6,197
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 50 of 84
            Pseudo Genes (internal stop)      :: 9 of 84
            Pseudo Genes (multiple problems)  :: 17 of 84
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:01:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6675808
            Orig. end                         :: 6686024
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047147.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="21"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1703..4000)
                     /locus_tag="GR131_RS28965"
                     /old_locus_tag="GR131_28965"
     CDS             complement(1703..4000)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640763.1"
                     /locus_tag="GR131_RS28965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_28965"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_015506778.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1e-35, bitscore:
                     121.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPADAPAAGTGSTAPPTPGGRRGRRAAPSSGRRRAAPPRRTPR
                     RRPVRTPTVLQMEAVECGAACLAMVLAHHGRHVPLEELRIACGVSRDGSRASNVLKAAR
                     SYGLTAKGMQMEPAALREVRAPAVLFWEFNHYVVHEGFLRRPGRRGAVRLNDPDKGRRV
                     VSAEEFDASFTGVALVLEPGPSFRKGGRKPGLLGALPARLRGTGATLLTALLASLLLVV
                     VGAALPALSRTYIDLFLIGERTSVLGPLFAAMGALVALTASLTALQQANLLRGRIISST
                     LSSARFLRHLLRLPVTFFAQRSPADLVQRLQSNDTVAETLARDLAAAAVDGVVVVLYAL
                     LLWTYDPQLTVVGVGIALLNIVAMRVVIHLRATRTQKLRADNARLTTTAYTGLQLIETM
                     KATGGEDGFFRRWAGQHATTLEEQQRLGVPTAVLSVVAPALATFNSALILWIGGLRAVE
                     GHLSVGLLVAFQALMVRFTAPLTRLNGVAGRIQDFAADVTRLRDVEAFPADGLYRREGA
                     GGGEADTRRLAGRVELEGLTFGYSPLDAPLLTEFSLTVGPGRQVALVGGSGSGKSTVSR
                     LLAGLYQPWEGTIRIDGQPLTEIPRSALAASVSFVDQDIFLFEGTVRENVTLWDPSLPD
                     EAVRTALRDAGLEEVIARRPDGIHARVEQDGRNFSGGQRQRLEIARALVRRPSVLVLDE
                     VTSALDAETEQRVMAALRRRGCACVIIAHRLSTVRDSDEIVVLDGGRVVERGRHAELVA
                     RGGAYAALVEEK"
     gene            complement(3997..4803)
                     /locus_tag="GR131_RS28970"
                     /old_locus_tag="GR131_28970"
     CDS             complement(3997..4803)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640761.1"
                     /locus_tag="GR131_RS28970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_28970"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_159313626.1"
                     /transl_table=11
                     /translation="MRFRQQALTKLQSPEQLDLPVRFARPQGLLVLAVTLVVMAAGGVW
                     AVTGSVATTLTAPGVLTHAQGSYVVQSTVSGQVTAVRTAEGRRVARGTPLLTVRTPRGD
                     VTIRALAAGRVTALAAATGAVVTTGSDLAAVERQENPDDPLLAVLYAPADSGASLRPGA
                     EVDLTVQSVPAQSYGVLRGTVKSVGRAARTRQQISADLGSTQLGEEFSRNGPPIAVEVR
                     LHPTKTTRSGYTWSTSDGPPQPLTTLTLVDGAVHLDEQRPADWLLP"
     gene            complement(5001..5216)
                     /locus_tag="GR131_RS28975"
                     /old_locus_tag="GR131_28975"
     CDS             complement(5001..5216)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="GR131_RS28975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_28975"
                     /product="hypothetical protein"
                     /protein_id="WP_008412458.1"
                     /sec_met_domain="strepbact (E-value: 4.4e-37, bitscore:
                     125.0, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYTPQVETAEIADSELDNISGGLVNGLVGSVTSSLDSVAPVSDT
                     LGTVTGVVEQTTGLNTAPVTGLVAGL"
     misc_feature    complement(5187..5202)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.07"
                     /tool="antismash"
     misc_feature    complement(5246..5266)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.44"
                     /tool="antismash"
     misc_feature    complement(5303..5318)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 16.77"
                     /tool="antismash"
     misc_feature    5684..5701
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.61"
                     /tool="antismash"
     gene            complement(5901..6407)
                     /locus_tag="GR131_RS28980"
                     /old_locus_tag="GR131_28980"
     CDS             complement(5901..6407)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640757.1"
                     /locus_tag="GR131_RS28980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_28980"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_031176719.1"
                     /transl_table=11
                     /translation="MRIRAVHQAELTALQDIERAAGVWFREIGMAEIAEDEPPPVAELA
                     RYARSGLAWVAADGEDRPVAYLVAERVDGNLHVEQVTVRPESARRGVGRALLDHAAGRA
                     RSEGAAALTLTTFTEVPWNAPYYVRCGFRPLAERELTPGLREIRRAEAAHGLDRWPRCC
                     MRREV"
     gene            complement(6632..8449)
                     /locus_tag="GR131_RS28985"
                     /old_locus_tag="GR131_28985"
     CDS             complement(6632..8449)
                     /NRPS_PKS="Domain: AMP-binding (23-474). E-value: 1.7e-88.
                     Score: 288.9. Matches aSDomain:
                     nrpspksdomains_GR131_RS28985_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412454.1"
                     /locus_tag="GR131_RS28985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_28985"
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="WP_030766608.1"
                     /sec_met_domain="AMP-binding (E-value: 5.2e-83, bitscore:
                     277.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREVSVERLVAPPVSGGLADSVFHTAATYPTRPQLALEPTKPGGP
                     WRTVTAARLRDEVLALARGLLAEGVRPGDRVVLMSRTRPEWTLLAYALWTLGAEVVPVY
                     PTSSIEQVRWVLADASVRAAVVEHSGQAMTIAAACADPGALLGLWQLDLDCTAELAERG
                     AEVPEDEVLRLRAAVRPEATAVICYTSGTTGAAKGCVISHANLAAECDTLIAGWGHLLA
                     PRGEQPSVLAFLPLAHCYGLMVVVTCVRGGYLLAHQPEMAPAVLLPALASFRPTFLFAV
                     PYIFERIFDEACREAEAAGHGVIFEKARAAAVAHAEAREKHTAGTGHGPGPVTRLRHSV
                     YERAVYAKVRAVFGGRVRSAVSGGSPLRRELGLFFQGAGVTVYDGYGLTETTAAVTAQP
                     PGRVRFGTVGRPLPGSTVRIAGDGEVWVRGDTVFRGYLHDPPGLADGWLATGDTGRFDP
                     DGYLVITGRKKDIIITSGGKSVSPQLLEEQLRRHPLIAQCLVVGDNRPYVAALLTVDQE
                     ALAHWQRLRGASGRAASAPADDTELRAQLQRAVNQANTLVSRAESIRAFEILPVDFTRQ
                     EGLMTPSFKLRRSAVTDTYAEVIEALYAR"
     aSDomain        complement(7028..8380)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR131_RS28985_AMP-binding.1"
                     /evalue="1.70E-88"
                     /label="GR131_RS28985_AMP-binding.1"
                     /locus_tag="GR131_RS28985"
                     /protein_end="474"
                     /protein_start="23"
                     /score="288.9"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="HTAATYPTRPQLALEPTKPGGPWRTVTAARLRDEVLALARGLLAE
                     GVRPGDRVVLMSRTRPEWTLLAYALWTLGAEVVPVYPTSSIEQVRWVLADASVRAAVVE
                     HSGQAMTIAAACADPGALLGLWQLDLDCTAELAERGAEVPEDEVLRLRAAVRPEATAVI
                     CYTSGTTGAAKGCVISHANLAAECDTLIAGWGHLLAPRGEQPSVLAFLPLAHCYGLMVV
                     VTCVRGGYLLAHQPEMAPAVLLPALASFRPTFLFAVPYIFERIFDEACREAEAAGHGVI
                     FEKARAAAVAHAEAREKHTAGTGHGPGPVTRLRHSVYERAVYAKVRAVFGGRVRSAVSG
                     GSPLRRELGLFFQGAGVTVYDGYGLTETTAAVTAQPPGRVRFGTVGRPLPGSTVRIAGD
                     GEVWVRGDTVFRGYLHDPPGLADGWLATGDTGRFDPDGYLVITGRKKDIIIT"
     CDS_motif       complement(7106..7183)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GR131_RS28985_0002"
                     /evalue="8.10E-06"
                     /label="NRPS-A_a6"
                     /locus_tag="GR131_RS28985"
                     /protein_end="448"
                     /protein_start="422"
                     /score="18.3"
                     /tool="antismash"
                     /translation="GEVWVRGDTVFRGYLHDPPGLADGWL"
     CDS_motif       complement(7841..7900)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GR131_RS28985_0001"
                     /evalue="1.10E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="GR131_RS28985"
                     /protein_end="203"
                     /protein_start="183"
                     /score="21.2"
                     /tool="antismash"
                     /translation="AVICYTSGTTGAAKGCVISH"
     gene            complement(8640..9539)
                     /locus_tag="GR131_RS28990"
                     /old_locus_tag="GR131_28990"
     CDS             complement(8640..9539)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506775.1"
                     /locus_tag="GR131_RS28990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_28990"
                     /product="hypothetical protein"
                     /protein_id="WP_201305800.1"
                     /transl_table=11
                     /translation="MRTVLRRSRSVPSRRPRGRVAVGAALAAAALFTAAPPALADTSPS
                     PSVSQDEEAEAGPTEAGTAFRTAARLAQGQRGTASASTGDYLYWVFPADTGQRPTVRAT
                     VSLPAEQARQSASTWRIDVYDGLRRRQPCTYGLQERRAAEDAAEVRLTCTLRTVRAYSE
                     PWANDPLPGAYYVRLTVSRLAADDLGRPFQAEVAVDSQETGGSTAVGGRLSEPLTPGIS
                     AVSAEEQGEEETSEEDGAEQAPRPAALAEPEDGWSSGWWTDRWLWTAGGAVLAALAGVF
                     GYTLTRGNGRPGRVPPGV"
     misc_feature    9653..9662
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 ctccaccagc ttgcgctgcc acttcacctg ccagagcccc agcagcagga agaccaccga
       61 gaccaccagc agcacgccga tcgccgccag cgccatcggc acgctgtgca ccagcagcag
      121 caccaggttg accccggcca ccgtcaccga ctgcacggcg gtcggcccga tccccgacag
      181 cacccggcgg atcgcgctga tccccatcgc ggcactggcc agctcaccgg tggagcggtc
      241 cgcgaagaag gccgtcggca gccgcagcag ccggtcccag acggccggct gcaacgccgc
      301 ctcgatccgc ccctccagac gcagcagcgt caacgactgg agcagcatga acgccgccga
      361 gaccaccccg gccaccatca ccgccacaca catctgcgcg atcagcccgg tctgcgcgct
      421 cggcacgtac gcgccgagca cctgcccggt cgccaccggc accagcgcgc ccagcgccac
      481 ggtgaccagg ccgctcagga cgaggttgcg caggtcgccc cgggagccgc gcaggctgaa
      541 gaacatcagc cggcgcagcg tcagcgggcc ctcgggaagc ggccggtaga gcatggtggc
      601 gcgttcctcg aactcggccg cctccttggc gccgacccgg atccgggcgc cggtcgccgg
      661 gttgagcgcc tcgtaccggc cgcgccgcca gagcagggcc accggcgcgc cggacctggc
      721 gcgccgcccc accagcggcc cggcgtcggt gcgccaccac tcgccggcca gccggaccgg
      781 gcgggtccgc agccgggcgg cggcggccat ccgctccacc gggtcgagcc gcccgccgcc
      841 cgcgaccgaa cgcgccgccg cctccggcag cgcgatgccg ctcgcccgcg ccacctggcg
      901 gaagaccgcg tacaccgcgt cgtcgcccgc ctcgccgcgc gccctcgggg agcggtcgcg
      961 gcggccgatc gagtcgagca gcgcctggtc ggcccgctcc cgtaccgcct caccggcgcg
     1021 gataccggcc gccgcccggt cctcgtgggc ccgctccagc cgctccaccc agccgtcgac
     1081 ggcggtcagc aggcggtact gctggtccac cagggactgc cacagcgccc cgtcgaccag
     1141 caggtcggcc gcggcctcgg cggcgaactc ggcccccagc cggacactgc ccggcgggac
     1201 gtccacccac agcacgtcct cgtcggcggg cccggacccc gccgcgggcc cgtccgcctg
     1261 cggtgccgcg aacagcacgg cgagcccccg gccgaggccg agccccaccg cgtgctccag
     1321 cggcccggcg accggctcgt acccggggta cccctcgtac ccctcggccc cctgcggcgc
     1381 gtacagctcg cgcagcggca ggcggcgtac ggcgcagcgg gaggagaggc ggcccaccag
     1441 ggtgtggcgg gggccctcga cggcgccgag caggagcgtg ccggcctcca ggcggccgag
     1501 gtagtgccag tggccgtcct cggcggccgc caccgcgaag aggtcgaggc tgccctcggt
     1561 gaccagccac agcgccgagg ggccgccgag cggcagggcc gaccctgcgg cgccctccac
     1621 cggtgcgccg agtccgccga gcgccgccgt caccgggtcg gcggccgggg tggggtggga
     1681 agcctgctgg gtgcccgtca tctcacttct cctcgaccag ggccgcgtac gcgccgcccc
     1741 gggcgaccag ttcggcgtgg cggccgcgtt ccacgacccg gccgccgtcc agcaccacga
     1801 tctcgtcgct gtcgcggacg gtggagaggc ggtgcgcgat gatcacgcag gcgcagccgc
     1861 gccgcctgag cgccgccatc acccgctgct cggtctccgc gtccagcgcg ctggtcacct
     1921 cgtccagcac cagcacgctc gggcggcgca ccagggctcg ggcgatctcc agccgctgcc
     1981 gctggccgcc ggagaagttc cgcccgtcct gctcgacccg tgcgtggatg ccgtcggggc
     2041 ggcgggcgat cacctcctcc agcccggcgt cgcgcagcgc ggtacggacc gcctcgtcgg
     2101 gcagcgaggg gtcccagagc gtgacgttct cccgcaccgt gccctcgaag aggaagatgt
     2161 cctggtcgac gaaggagacc gaggcggcca gcgcggagcg cgggatctcg gtgagcggct
     2221 gcccgtcgat gcggatggtg ccctcccacg gctggtacag gcccgcgagc agccgggaga
     2281 ccgtcgactt gccgctgccc gacccgccga ccagcgccac ctgccgcccc ggtccgaccg
     2341 tcagcgagaa ctccgtcagc agcggcgcgt ccagcgggct gtaaccgaag gtcagccctt
     2401 ccagctccac ccggcccgcc agccgccggg tgtccgcctc gccgccgccg gccccctccc
     2461 gccggtacag cccgtccgcc gggaacgcct cgacgtcccg cagccgggtc acgtcggccg
     2521 cgaagtcctg gatgcggccc gcgacgccgt tgagccgggt cagcggcgcg gtgaaccgca
     2581 ccatcagcgc ctggaaggcc accagcagcc cgaccgacag atgcccctcg accgcccgca
     2641 gcccgccgat ccacaggatc agcgcgctgt tgaaggtggc gagcgccggg gccaccaccg
     2701 acagcaccgc cgtcggcacc ccgagccgct gctgctcctc tagcgtcgtc gcgtgctgcc
     2761 ccgcccacct gcggaagaac ccgtcctcgc cgccggtcgc cttcatcgtc tcgatgagct
     2821 ggagcccggt gtacgcggtg gtggtgagcc gcgcgttgtc ggcccgcagc ttctgcgtcc
     2881 gggtggcccg caggtggatc accacccgca tcgccacgat gttgagcagc gcgatcccca
     2941 ccccgaccac ggtcagttgc gggtcgtacg tccacagcag cagcgcgtac agcaccacca
     3001 ccaccccgtc gacggcggcg gccgccaggt cgcgggcgag ggtctcggcc accgtgtcgt
     3061 tcgactggag ccgctgcacc aggtcggcgg gggagcgctg ggcgaagaag gtcaccggca
     3121 gccgcagcag atggcgcagg aaccgggcgc tgctcagcgt cgaggagatg atccgcccgc
     3181 gcagcagatt ggcctgctgg agggcggtca gcgaggcggt cagcgccacc agcgcgccca
     3241 tcgccgcgaa gagcggcccg agcaccgagg tccgctcccc gatcaggaac aggtcgatgt
     3301 acgtccggct cagcgcgggc agcgccgcgc ccacgacgac cagcagcagg ctggccagga
     3361 gcgcggtcag cagggtggca ccggtgccgc gcagccgggc cgggagcgcg ccgagcagcc
     3421 cgggcttgcg gccgcccttg cggaacgagg ggcccggctc caggaccagg gcgacgccgg
     3481 tgaacgaggc gtcgaactcc tcggcgctca ccacccggcg tcccttgtcg gggtcgttga
     3541 ggcggaccgc gccgcgccgc ccgggacgcc gcaggaaccc ctcgtggacg acgtagtggt
     3601 tgaactccca gaacagcacg gcgggggccc gcacctcgcg cagggcggcc ggttccatct
     3661 gcatgccctt ggcggtcagg ccgtaactgc gggccgcctt caggacgttg ctggcgcgtg
     3721 agccgtcgcg ggagacgccg caggcgatcc gcagctcctc cagcgggacg tgccgcccgt
     3781 ggtgggcgag gaccatggcg agacaggcgg cgccgcactc caccgcctcc atctgcagga
     3841 cggtgggggt gcggacgggc ctccgccggg gcgtacggcg cgggggcgcg gcgcggcggc
     3901 ggccggagga gggggcggcg cggcggcccc ggcggccgcc cggggtgggc ggggcggtgg
     3961 agccggtccc ggcggcgggg gcgtcggcgg gtgcggtcac gggaggagcc aatcggccgg
     4021 gcgctgttcg tccaggtgga cggcgccgtc gacgagggtg agggtggtca gcggctgcgg
     4081 cgggccgtcg gaggtcgacc aggtgtagcc gctgcgggtg gtcttcgtgg ggtgcagccg
     4141 gacctcgacg gcgatcggcg gcccgttccg ggagaactcc tcgccgagct gggtggagcc
     4201 gaggtcggcg gagatctgct gccgggtccg ggcggcgcgg ccgaccgact tcacggtgcc
     4261 acgcagcacg ccgtagctct gggcgggcac cgactgcacg gtgaggtcga cctcggcgcc
     4321 gggccggagg gaggcgccgc tgtcggcggg ggcgtagagc accgcgagga gggggtcgtc
     4381 cgggttctcc tggcgctcga cggcggccag gtcggagccg gtggtgacga cggcgccggt
     4441 ggcggcggcc agtgcggtga cccgcccggc ggccagcgcg cggatggtca cgtcgccccg
     4501 cggggtgcgg acggtcagca gcggggtgcc gcgggcgacg cggcggccct cggcggtgcg
     4561 gacggcggtg acctgtccgg agacggtgga ctggacgacg tagctgccct gggcgtgggt
     4621 gaggacgccg ggcgcggtga gggtggtggc gaccgagccg gtgacggccc acacgccgcc
     4681 ggcggccatc acgacgaggg tgacggcgag gaccaggagt ccctgcgggc gggcgaaccg
     4741 caccggcagg tccagctgtt cgggcgactg gagcttggtc agggcctgtt ggcgaaagcg
     4801 cacggaagac ttcctggact gccgggcacc ggcctctcgg ccgtgcggcc gggtgtcgcc
     4861 gtggtgcggg gggacgccgc gcgggcgtcc ggggaggaca aaggaggcgg ccggtgcccc
     4921 ggaacccgag cgggtccggg gcaccggccg agcggtccgc ctcagcgggc cgcacgcgtc
     4981 gtcaggcgac gcgtggggtc tcagagaccg gcgacgaggc cggtgaccgg agcggtgttc
     5041 agaccggtgg tctgctccac gacgccggtg acggtgccga gggtgtcgga gaccggggcg
     5101 accgagtcga ggctggaggt gaccgagccg acgaggccgt tcacgaggcc gccggagatg
     5161 ttgtccagct cgctgtcggc gatctcggcg gtctcgacct ggggggtgta gctcatgggg
     5221 ggagttccct tcgcatcact gacggaaata gtcaccgcga cgcactcgtg cagaagaagg
     5281 ggaaaatctt ctggcggccc ccgggcgaaa ggcgtgaacc ttcagatccg ggggcggtgc
     5341 cgcagtgcga tggatcaaag cacgacggtg cgcggggcgg ccaatcggtg agagcggaat
     5401 tcggccggcg gtgccgttct cctcaccacc gtgtgcggaa tcgggcacag gagaacggca
     5461 ccaccttcac accgtcccgc cgccgtacca ggtggggtca cgctcgcgtg tacaccgcgc
     5521 ccctcgctgt gaagccctgg tgcacgccgt cgacctcggc cgttcacgcc cggtgaaggt
     5581 atgggtggcg gtgacggagc tgcggcacgg ctgtgcagat tcaccgcagg agaacccgct
     5641 ccggggtgcg gcggattcgt gtgcggacgg ctctgagaag gccatgtttc caacatgtgg
     5701 aggaacagcg tccgccacga ggagaagaag gagcggaagc ggactgattg ccttcgcccg
     5761 gcgaattctt gcgcactgag aaaagagatc gaaggtgcag gggcgggaat ttcccacagg
     5821 gcgcgaaagg gcggggacgg ggaagcgtgc attccccgtc cccgcccttt cgcgccccgc
     5881 gctctcctcg gcgcccgcgc tcacacctcc cggcgcatgc agcagcgcgg ccaccggtcc
     5941 agtccgtgcg ccgcctcggc ccgccggatc tcccgcagcc ccggcgtcag ctcgcgctcc
     6001 gccagcgggc ggaagccgca ccgcacgtag tacggcgcgt tccacggcac ctcggtgaag
     6061 gtggtgagcg tcagcgccgc ggccccctcg ctccgggccc gcccggccgc gtgatccagc
     6121 agggcccgcc cgacgccccg gcgcgcactc tccggacgca ccgtcacctg ctcgacgtga
     6181 aggttgccgt cgacccgctc cgcgaccagg tacgccacgg gccggtcctc cccgtcggcc
     6241 gccacccagg cgagccccga ccgcgcgtac cgcgccagct ccgccaccgg cggcggctcg
     6301 tcctcggcga tctcggccat gccgatctcc cggaaccaga ctccggcggc ccgttcgatg
     6361 tcctggaggg ccgtcagttc ggcctggtgc acggctcgga tacgcatggg ggcatggtcc
     6421 ggtgcgcggg ggcgggaggc aacggaaaag ggggcggccc caccggacga agggggggag
     6481 agtccggtgg ggccgaggca acctgccagg ccagggggaa gggccgtcag gtcgttctga
     6541 ggggcgtgtg cccggggcgg ggggagtcat gccggccggg ggcgggcaat tcttcgcgtg
     6601 gggttctcgc ggcccgcccc gcccccgccg ttcagcgggc gtacagggcc tcgatgacct
     6661 ccgcgtaggt gtccgtgacg gcgctgcggc ggagtttgaa cgagggggtc atcaggccct
     6721 cctggcgggt gaagtcgacc ggcaggatct cgaaggcccg gatcgactcg gcgcgtgaga
     6781 ccagggtgtt ggcctggttg accgcccgct ggagctgggc ccgcagctcc gtgtcgtccg
     6841 ccggggccga ggcggcccgg ccggacgccc cgcgcaggcg ctgccagtgg gccagggcct
     6901 cctggtcgac ggtgagcagg gccgccacgt agggccggtt gtcgccgacc accaggcact
     6961 gggcgatcag cggatggcgg cgcaactgct cctccagcag ttgcggggag acgctcttgc
     7021 cgccgctggt gatgatgatg tccttcttgc ggccggtgat caccagatag ccgtccgggt
     7081 cgaagcggcc ggtgtccccg gtggccagcc agccgtcggc gagccccggc gggtcgtgca
     7141 gataaccccg gaagaccgtg tccccgcgta cccacacctc gccgtcgccg gcgatccgga
     7201 cggtcgagcc cggcagcggg cgccccacgg tgccgaagcg gacccggccc gggggctggg
     7261 cggtcaccgc cgccgtggtc tcggtcagcc cgtagccgtc gtagacggtc acccccgcgc
     7321 cctggaagaa gagccccagt tcgcggcgga ggggcgagcc gcccgagacg gccgagcgga
     7381 cccgtccgcc gaagaccgcc cggaccttgg cgtagacggc ccgctcgtag acggagtgcc
     7441 gcagccgggt gaccggtccc gggccgtgtc cggtgcccgc cgtgtgcttc tcccgggcct
     7501 cggcgtgggc cacggcggcc gcccgcgcct tctcgaagat caccccgtgc ccggcggcct
     7561 cggcctcccg gcacgcctcg tcgaagatcc gctcgaagat gtaggggacg gcgaagagga
     7621 aggtgggccg gaacgaggcg agggccggga ggagcaccgc cggggccatc tccggctggt
     7681 gggcgaggag atagccgccg cgtacgcagg tgaccaccac catcaggccg tagcagtggg
     7741 ccagcggcag gaaggcgagg accgagggct gttcgccgcg gggggccagc aggtggcccc
     7801 agcccgcgat gagggtgtcg cactcggcgg ccaggttggc gtggctgatc acgcagccct
     7861 tggcggcgcc ggtggtgccc gaggtgtagc agatgacggc ggtcgcctcc gggcggaccg
     7921 ccgcccgcag ccgcagcacc tcgtcctccg gcacctcggc gccgcgctcg gcgagttccg
     7981 ccgtgcagtc caggtccagc tgccagagcc cgagcagggc gccggggtcg gcgcaagccg
     8041 ccgcgatcgt catcgcctgc ccgctgtgct ccaccaccgc cgcccggacg gacgcgtcgg
     8101 cgaggaccca gcggacctgc tcgatggagg aggtggggta gaccggcacc acctcggcgc
     8161 ccagcgtcca cagggcgtac gccagcagcg tccactcggg ccgggtacgg gacatcagca
     8221 ccacccggtc gccgggccgc actccctcgg ccagcaggcc ccgggccagt gccagtacct
     8281 cgtcgcggag ccgggccgcc gtgacggtgc gccaggggcc gccgggcttt gtgggttcca
     8341 gggccagctg ggggcgcgtc gggtacgtcg cggcggtgtg gaagaccgag tcggccaggc
     8401 cgccggagac cggcggggcg accaggcgct ccacactcac ttcccgcacc gggtacgcct
     8461 ccgttcgtca tcccggccgc gtgccgcacc agcgcggggc ccgtacctga cggatggccg
     8521 gctcggcaac ctacagcgcc ccgcacgcac tggccagggg tgacggggga gttcaccacc
     8581 gtgcgccggg ggcggctccg cgcgccgccc ggccggggac gcgcggagcc gtcggcccgt
     8641 cagacgccgg gcggcacccg gccgggacgc ccgttgcccc gggtcagggt gtagccgaag
     8701 acgcccgcca gggcggccag tacggcaccc ccggccgtcc acagccaccg gtcggtccac
     8761 cagccggagg accagccgtc ctcgggctcg gcgagcgccg ccggacgcgg cgcctgctcg
     8821 gcaccgtcct cctcggacgt ctcctcctcg ccctgctcct cggcggagac cgccgagatg
     8881 ccgggcgtca gcggctcgga gagcctcccg ccgaccgccg tggagccgcc ggtctcctgg
     8941 gagtcgaccg ccacctcggc ctggaacggc cggcccaggt cgtcggcggc gagccgcgag
     9001 accgtcagcc ggacgtagta cgccccgggc agcgggtcgt tggcccacgg ctcggagtag
     9061 gcccgcacgg tacggagcgt gcaggtcagc cgcacctcgg cggcgtcctc ggcggcccgc
     9121 cgctcctgga ggccgtaggt gcacggctgg cggcggcgga gcccgtcgta gacgtcgatc
     9181 cgccaggtgg aggcggactg ccgggcctgt tcggcgggga gcgacacggt cgcccgcacg
     9241 gtcgggcgct gcccggtgtc ggccgggaag acccagtaga ggtagtcacc ggtcgacgcg
     9301 gaggcggtgc cgcgctgccc ctgcgcgagc cgcgccgccg tgcggaacgc ggtgcccgcc
     9361 tcggtcggac cggcctcggc ctcctcgtcc tgcgagacgg agggcgaggg cgaggtgtcg
     9421 gcgagggccg gcggcgcggc cgtgaacagc gccgccgcgg cgagggcggc ccccacggcg
     9481 acgcgcccgc gcgggcgccg ggacggcacg gaccgggacc tgcgaagaac ggtacgcatc
     9541 agtgggtcct ccagacggcg aagcgccagc gcgacagcca gccgaagatc aggccgccga
     9601 ggaaaccggc gagcaccagc acgcccagca gccaccagcc acggcccagc ccgaaggcgg
     9661 ccgtgtccga ggcgtcggac ggcccgtcga ccacgtcgac cgtcagctcc agcggcatac
     9721 ccggcgccga ggcgtcggcg gccttggcgg aggccggggc gaaggagttg ctgacctcca
     9781 ggcagaccgg ggtcgcggcc ggcttgtcgg tgtcggcgtc ctcccgctcc cgctccgggt
     9841 ggcgcagacc ggacgagatc acgtcggtcc ggccgctgcc cgcctccgag ccgcgcacca
     9901 gctcgcggcc gtgcgcggtc accgcgcgca gcagcacgcc gtagtcgtcg gcgagcgggc
     9961 ggtcggccgc cacactcacc gaggcgcgca gctcctggcc gggcagcacg tccacccggt
    10021 accagcggtg ggtgccgagc ggctggcggt cggtgtacag gcccggctcg atcttcggcg
    10081 cgtcggcgca ggccgtggcg ccctcgaccg gcaccggcgt gaccaccggg tcggccgcgc
    10141 ggtccaccag ctgcttcacc ttgtcggaga ggtcctcctt gtgctgcacg gcggtgtagg
    10201 tgccgccggt cgctcc
//
