LOCUS       NZ_CP047147            11329 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. GF20 chromosome, complete genome.
ACCESSION   NZ_CP047147
VERSION     NZ_CP047147.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GF20
  ORGANISM  Streptomyces sp. GF20
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7311137)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7311137)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: SPAdes v. Nov-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:38:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,372
            CDSs (total)                      :: 6,281
            Genes (coding)                    :: 6,197
            CDSs (with protein)               :: 6,197
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 50 of 84
            Pseudo Genes (internal stop)      :: 9 of 84
            Pseudo Genes (multiple problems)  :: 17 of 84
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:01:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6059517
            Orig. end                         :: 6070846
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047147.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11329
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="20"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11329
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11329
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6329](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6329)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            860..2332
                     /gene="hemG"
                     /locus_tag="GR131_RS26165"
                     /old_locus_tag="GR131_26165"
     CDS             860..2332
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404755.1"
                     /locus_tag="GR131_RS26165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_26165"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_159313342.1"
                     /sec_met_domain="DAO (E-value: 1.4e-08, bitscore: 33.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAETEGNPAGAGGASPRVVVVGGGVAGLTAALRLLDAGAGVTLL
                     EASERLGGKLRTGEIGGVRVDLGAESMLARRPEAVGLAREAGLADALQPPATASASLWT
                     RDALRPMPKGHVMGVPADPASLAGVLSEEGVARIAEDRALPRTEVGEDIAVGEYVAARV
                     GREVVDRLVEPLLGGVYAGNAYRISLRAAVPQLFAAAREHTSLTEGVRAIQARTAADPR
                     TDPVFMGIDGGVGRLPLAVADTVRARGGRIRTGTPVTGLARVPGGWRLTTGTPDAPESL
                     EADAVVLAVPAPQAARLLAAELPAASADLAEIEYASMALMTFAFRADEAELPQGSGFLV
                     PPVDGRTVKAATFAGRKWRWIAEQEPGLHVVRTSVGRHREEKILGRDDAGLLALARRDL
                     KAATGVDAEPVDAHVTRWDQGLPQYPVGHLARVARVRDQVAKAPGLALCGAAYDGVGIP
                     ACVASATEAARQVMAARPQGDPASGPSGHAGE"
     gene            2337..3053
                     /gene="hemQ"
                     /locus_tag="GR131_RS26170"
                     /old_locus_tag="GR131_26170"
     CDS             2337..3053
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951588.1"
                     /locus_tag="GR131_RS26170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_26170"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_159313343.1"
                     /transl_table=11
                     /translation="MSAAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRSGYAD
                     EVQDLFDKLAAKDVTVRGTYDVSGLRADADVMIWWHAETADALQEAYNLFRRTRLGRAL
                     EPVWSNTALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYEWYLLPDEERRAMLADH
                     GKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARRHVREEVPF
                     YTGRRKSVADLVAQLA"
     gene            complement(3067..4713)
                     /locus_tag="GR131_RS26175"
                     /old_locus_tag="GR131_26175"
     CDS             complement(3067..4713)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-479). E-value:
                     3.4e-20. Score: 64.6. Matches aSDomain:
                     nrpspksdomains_GR131_RS26175_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404759.1"
                     /locus_tag="GR131_RS26175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_26175"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_159313344.1"
                     /sec_met_domain="PF00561 (E-value: 2.4e-23, bitscore: 81.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAPLILTAAGALLLTPLAASPQAPAAGAPAPPGGPEARGVALAA
                     ERAARTPIAFGPCPDEEGLPRSLECGTVRVPLDYARPDGEQIDLTVSRAKAAGPKNERQ
                     GALVFNPGGPGSSGMLFPLVGIFPEWKRLASVYDLVGYAPRGVGRSAPLSCRDPREKPD
                     GNWQAPEHPTEKQKAGRKAEAEAYARGCAERGGKRLRHIHSLNNARDLDVVRAGLGERK
                     LTFMGASYGSYFGALYAKLFPSHVRRMVFDSAVDPSPSRVWYRNNMAQSAAFEKRWADF
                     RRWIASHDAAYGMGTDEAQVLAGYRRAREKLAAEPAGGTVGPAQLQAVMLRAQYYEDYW
                     PSHAEALAAYLKGDPQPLVNLATPLEEGRVEAENARAVYTAVECNDAPWPTSWQTWDRD
                     HTRLARTAPFETWENAWMNLPCAYWKEPRQTPMDVRVAPGELPPTLILAGERDAATPYA
                     GALELRRRQPDAALVTERGAGTHGLAGGPNACVNRHVEAYLIDGKLPARSTDCAARPAP
                     EPEAQEAGTRAAGSGARPALPRVAGWSPAAR"
     aSDomain        complement(3277..4398)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR131_RS26175_Abhydrolase_1.1"
                     /evalue="3.40E-20"
                     /label="GR131_RS26175_Abhydrolase_1.1"
                     /locus_tag="GR131_RS26175"
                     /protein_end="479"
                     /protein_start="105"
                     /score="64.6"
                     /tool="antismash"
                     /translation="ALVFNPGGPGSSGMLFPLVGIFPEWKRLASVYDLVGYAPRGVGRS
                     APLSCRDPREKPDGNWQAPEHPTEKQKAGRKAEAEAYARGCAERGGKRLRHIHSLNNAR
                     DLDVVRAGLGERKLTFMGASYGSYFGALYAKLFPSHVRRMVFDSAVDPSPSRVWYRNNM
                     AQSAAFEKRWADFRRWIASHDAAYGMGTDEAQVLAGYRRAREKLAAEPAGGTVGPAQLQ
                     AVMLRAQYYEDYWPSHAEALAAYLKGDPQPLVNLATPLEEGRVEAENARAVYTAVECND
                     APWPTSWQTWDRDHTRLARTAPFETWENAWMNLPCAYWKEPRQTPMDVRVAPGELPPTL
                     ILAGERDAATPYAGALELRRRQPDAALVTERGAG"
     gene            complement(5001..6329)
                     /locus_tag="GR131_RS26180"
                     /old_locus_tag="GR131_26180"
     CDS             complement(5001..6329)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641256.1"
                     /locus_tag="GR131_RS26180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_26180"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_159313345.1"
                     /sec_met_domain="DUF692 (E-value: 4.5e-94, bitscore: 313.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPAIAEAVEAMAGIEWVEVVAENVCPGHLPDSLRR
                     LRERGVAVVPHGVGLGLGGAERPDPVRLAALAERAEALGSPLVTEHIAFVRAGGERTAS
                     RGLEAGHLLPVPRTREGLAVLCENVRIAQDQLPVPLAVENIAALFSWPGEELTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNQGEDPAATLDALPLEALAYVHVAGGFERDGVWHDSH
                     AHPVPEPVLAVLAELASRVRPPGVLLERDERFPSAAELEAEVAKIRRTVAVAGRDRVPV
                     GAGARPGSVPASGAGAATGPVADEAARTRLALGQTALLSALVAGTPAPEGFDRGRLRVQ
                     SRMLTAKRGSVVASLAPELEQILGDTYRPGFLRYAAGRPLTGGYRADALAFALHTLREE
                     PPAERAVRRALRDWWIERSGRASLVGRPWARWARVARFALLGR"
     gene            complement(6440..7153)
                     /locus_tag="GR131_RS26185"
                     /old_locus_tag="GR131_26185"
     CDS             complement(6440..7153)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894649.1"
                     /locus_tag="GR131_RS26185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_26185"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_008404765.1"
                     /transl_table=11
                     /translation="MTSEPLSDSPFRDERTARDEAPQFVLPLVVRLERAEPPALTDALQ
                     TAARAVLTLLSDPRSEGDGVWAEAVRDWQDARIRKVVRRARGAEWRKAAALPGITVEDG
                     GAEVRVYPPVPLDGWPRELAKLQVSGTELEDPEEPGEPAPGVPVLWLNPDVPMSAGKAM
                     AQAGHGAQLAWWQLSGAERDAWREQGFPLAVRTAGAERWKELVESGLPVVRDAGFTEIA
                     PGSCTVVAEHPALVG"
     gene            complement(7184..8053)
                     /locus_tag="GR131_RS26190"
                     /old_locus_tag="GR131_26190"
     CDS             complement(7184..8053)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951592.1"
                     /locus_tag="GR131_RS26190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_26190"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_003951592.1"
                     /transl_table=11
                     /translation="MVRKAKLRTGTALGALMALGAVAGCGQSVPDPHDTAPGPRASHSA
                     SPAAPTAPPTLAPGPGGLAPVFANGPRNDGKTVALTFDADMTADQGERAAGGERFDHPE
                     LIASLRRLKVPATVFMTGRWAEEYPDQARAIGADPLFEIANHSYSHHAFTGDCYGLPRV
                     AESEMRADVERAYTAFRTAGVKKAMPYFRFPGGCYDDAALREVSAAGVTAVQWDVVSGD
                     AFATDADAVAQQVLDGVRPGSVVVMHCTRSAAPTTERALRRIVPALREQGYRFVKVSEL
                     IGAASRKG"
     gene            complement(8180..8785)
                     /locus_tag="GR131_RS26195"
                     /old_locus_tag="GR131_26195"
     CDS             complement(8180..8785)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951593.1"
                     /locus_tag="GR131_RS26195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_26195"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_159313346.1"
                     /transl_table=11
                     /translation="MSDNGAAQPDLRDRRRDSVRREIALHAVDLFERQGFAGTTVEQIA
                     EATGVSLRTFYRHCPVKEEALTPLLAEGVDALVAELARRPAGEPLTTAARAALLHSCRR
                     SGSQTRRTARVMLGVPALKARWLAAGRRAQGLLAPLVAERTGTPADALAPAVTAGLLIN
                     VATTAVEHWACGDESLTLESVTADAFAVVTGFGATGQD"
     misc_feature    complement(8861..8877)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.38"
                     /tool="antismash"
     misc_feature    complement(8939..8959)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.03"
                     /tool="antismash"
     gene            8950..9864
                     /locus_tag="GR131_RS26200"
                     /old_locus_tag="GR131_26200"
     CDS             8950..9864
                     /NRPS_PKS="Domain: PKS_KR (9-189). E-value: 4.2e-18. Score:
                     57.8. Matches aSDomain:
                     nrpspksdomains_GR131_RS26200_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818912.1"
                     /locus_tag="GR131_RS26200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_26200"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_159313347.1"
                     /sec_met_domain="adh_short (E-value: 4.3e-31, bitscore:
                     106.8, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGISFEGRVAIVTGAGGGLGRAHALDLAARGARVVVNDLGGDIS
                     GSGGGTSMADQVVREIVAAGGEAVANYDSVATPEGGRAIVQRAVDAFGTVDILVNNAGN
                     IRNAPFTELTPEAIEALLAVHVKGGFYVTQPAFAVMREKGYGRIVFTASAAGVFGSPEQ
                     ANYAAAKSSVVGLANVVALEGAPHGIKVNTILPVSESRMIADMNPETLAGVPRTPAHRE
                     PEMITAMVSYLASEANPYTREMFSIARGRYARVFVGVAEGHQVPYEAPVATADDVAAHI
                     DRIRDQEGYHVPGSMVEEFSLVP"
     aSDomain        8977..9516
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR131_RS26200_PKS_KR.1"
                     /evalue="4.20E-18"
                     /label="GR131_RS26200_PKS_KR.1"
                     /locus_tag="GR131_RS26200"
                     /protein_end="189"
                     /protein_start="9"
                     /score="57.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAIVTGAGGGLGRAHALDLAARGARVVVNDLGGDISGSGGGTSMA
                     DQVVREIVAAGGEAVANYDSVATPEGGRAIVQRAVDAFGTVDILVNNAGNIRNAPFTEL
                     TPEAIEALLAVHVKGGFYVTQPAFAVMREKGYGRIVFTASAAGVFGSPEQANYAAAKSS
                     VVGLANVVALEGAPHGI"
     CDS_motif       8983..9060
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GR131_RS26200_0001"
                     /evalue="3.10E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="GR131_RS26200"
                     /protein_end="37"
                     /protein_start="11"
                     /score="16.6"
                     /tool="antismash"
                     /translation="IVTGAGGGLGRAHALDLAARGARVVV"
     CDS_motif       9394..9456
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GR131_RS26200_0002"
                     /evalue="4.80E-08"
                     /label="PKSI-KR_m4"
                     /locus_tag="GR131_RS26200"
                     /protein_end="169"
                     /protein_start="148"
                     /score="25.5"
                     /tool="antismash"
                     /translation="VFTASAAGVFGSPEQANYAAA"
     gene            complement(9937..11301)
                     /locus_tag="GR131_RS26205"
                     /old_locus_tag="GR131_26205"
     CDS             complement(9937..11301)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951595.1"
                     /locus_tag="GR131_RS26205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_26205"
                     /product="PhoX family protein"
                     /protein_id="WP_159313348.1"
                     /transl_table=11
                     /translation="MSATRRQVLARTGAVAGIAFTGSLSELFAGTAAAKGMGKAGYGPL
                     VPDPKGLLDLPKGFRYRVLSREGDRMLSGEGQVPSNHDGMSAFGGRRGQVHLVRNHENR
                     PSAPIRVPPVRGLTYDPEGMGGCTALTLDPGHRVLGERVAIAGTSTNCAGGPTPWGTWL
                     TCEETEDKAGTNGYTKDHGFIFEVDPVDPHRTGAVPLKAMGRFQHEAIAIDPRHGIVYE
                     TEDAFERPFGLFYRFLPEKPRGGLGSLRAGGALQAMRVPGVPDLSAIQETGASFDRVEW
                     VDVPDPLAEETPIRFQDFGPKGITHAQKLEGCYWGGRSVYFVSSFARSSEGSAADHYGQ
                     IWRYDPDRRRLTLVIVFGPDTDVQLPGEEPDNICLAPSGGLMVAEDGEGAQHVYGVTTR
                     GEVYPMARGRQNIGTPEEPAWGEFAGVCFSPDYDTMFVNCYTPGTTFAVTGPWHR"
ORIGIN
        1 gtgaccgtcg ccatcgaggt gcggtccttc agcgaggact gctgggccag cagcgcctcc
       61 agctccatct ggcggctgct cagctcgccc tccagcgaga cgatgtcgct cagcttctcc
      121 gcccggtcca tcagctcccg gacccgcgcg acgctggcgc gctgcgactt cagccggctc
      181 tcgacgtcca ccacctcctc ggtgacgtcc tccgcgctgg tccgccggga caccacctcg
      241 ccgccctcgc cgagcgagtc cagcaccgcg tcgtacttct cctgcgggac gcggaccacc
      301 acggtcgacc gctcgtggtc ctgggagtcg cgccgggtcg actcgttgcc gacgtagccg
      361 cccgcgccct ccaccaccga gcgggccgag gccagcgcgg cgggcacatc ctccacccgc
      421 aggtgcagct cggccgtccg gaccaggtgg acgcccttca gctgggggcg cgccttcccg
      481 tccgacgcct cgtccgccga ctcccgcttc gcgctgtcgg cctgaccgcc gcccgcgccg
      541 tcggcggccg acggcgcgac cgcgcggtcc cccgccgacg acgcgccgga gtccggcccg
      601 gccgagcacc ccgccagccc cagtaccgcc accgccagca ccacggccgc ccgccgcccc
      661 gcgatccggc cgccactcga tccccgcatc cggctcatcc gccgcccccc ttgtccttcc
      721 gcgcccaccc ctcgggggcg cctgtgccgc tacgacggag cagcgcaccg gtgggtttcg
      781 cctcccgggt cgcgaaccgg taacggaagc gcgccgcccc ggcggccgcc tgccgcgtcc
      841 ggtttgagac agtggctcca tgcgcgcgga aacagaggga aacccggcgg gagcgggtgg
      901 cgcgagcccc cgtgtggtgg tcgtcggagg cggcgtcgcg gggctcacgg ccgcgctgcg
      961 cctgctcgac gccggggccg gcgtcaccct cctggaagcc tccgagcggc tcggcggcaa
     1021 gctccgcacc ggcgagatcg gcggcgtccg cgtcgacctg ggcgccgagt cgatgctggc
     1081 ccgccgcccc gaggcggtcg gcctagcccg tgaggcggga ctggccgacg ccctccagcc
     1141 ccccgccacc gccagcgcct ccctgtggac ccgcgacgcc ctgcgcccca tgcccaaggg
     1201 ccacgtcatg ggcgtccccg ccgacccggc cagcctcgcc ggggtcctct cggaggaggg
     1261 cgtcgcgcgg atcgccgagg accgcgccct gccccgtacc gaggtcggcg aggacatcgc
     1321 cgtcggcgag tacgtcgccg cccgggtcgg ccgcgaggtc gtcgaccggc tcgtcgaacc
     1381 cctcctcggc ggcgtctacg ccggcaacgc ctaccgcatc tcgctgcgcg ccgccgtccc
     1441 ccagctcttc gccgccgccc gcgagcacac ctccctgacc gagggggtcc gcgcgatcca
     1501 ggcccgcacc gccgccgacc cgcgcaccga ccccgtcttc atgggcatcg acggcggggt
     1561 cggccgcctc ccgctcgccg tcgccgacac cgtccgcgcc cgcggcggcc gcatccgcac
     1621 cggtacgccc gtcaccggcc tcgcccgcgt ccccggtggc tggcggctga ccaccggcac
     1681 ccccgacgcg cccgagtccc tggaggccga cgccgtcgtc ctcgccgtcc ccgcgcccca
     1741 ggccgcccgg ctgctcgccg ccgaactccc ggccgcctcc gccgacctcg ccgagatcga
     1801 gtacgcctcg atggccctga tgaccttcgc cttccgcgcg gacgaggccg agctgccgca
     1861 gggcagcggc ttcctcgtgc cgccggtcga cggacgcacc gtcaaggccg ccaccttcgc
     1921 gggacgcaag tggcggtgga tcgccgagca ggaaccgggg ctgcacgtcg tccgcacctc
     1981 ggttggccgc catcgcgagg agaagatcct cggccgcgac gacgccggcc tcctcgccct
     2041 cgcccgacgc gacctcaagg ccgccaccgg cgtcgacgcc gagcccgtcg acgcccacgt
     2101 gacccgctgg gaccaggggc ttccccagta cccggtcggc cacctcgccc gcgtcgcccg
     2161 cgtccgcgac caggtcgcca aggcccccgg cctcgccctg tgcggcgccg cctacgacgg
     2221 tgtcggcatc cccgcctgcg tcgcctccgc caccgaggcg gcccgccagg tcatggcggc
     2281 ccgcccacag ggcgacccgg cgtcgggccc gtccgggcac gcgggagaat gaccccatga
     2341 gtgctgctcc cgagaagatc cccaacgcgg gcaagaaggc caaggacctc aacgaggtca
     2401 tccgctacac cctgtggtcc gtcttcaagc tccgcgacgt gctccccgag gaccggtccg
     2461 gctacgccga cgaggtccag gacctgttcg acaagctcgc cgccaaggac gtcaccgtcc
     2521 gcggcaccta cgacgtctcc ggtctgcgcg ccgacgccga cgtgatgatc tggtggcacg
     2581 ccgagaccgc cgacgccctc caggaggcgt acaacctctt ccgccgcacc cgcctcgggc
     2641 gcgcgctgga gccggtctgg tcgaacacgg cgctgcaccg ccccgccgag ttcaacaagt
     2701 cgcacatccc ggcgttcctc gccgacgaga ccccccgcga ctacatcagc gtctacccgt
     2761 tcgtccgctc ctacgagtgg tacctcctcc ccgacgagga gcgccgggcg atgctcgccg
     2821 accacggcaa gatggcgcgc ggctacccgg acgtccgcgc caacacggtc gcctcgttct
     2881 ccctcggcga ctacgagtgg atcctcgcct tcgaggccga cgagctgtac cgcatcgtcg
     2941 acctcatgcg tcacctgcgc gcctccgagg cccgccgcca cgtccgcgag gaggtgccct
     3001 tctacaccgg gcgccgcaag tccgtcgccg acctggtcgc ccagctcgcc tgagccccgc
     3061 tccgcctcac cgggccgccg gggaccaccc ggcgacccgg gggagcgccg gccgcgcccc
     3121 gctcccggcg gcccgcgtcc cggcctcctg cgcctccggc tcgggggccg gccgggccgc
     3181 gcagtcggtg gagcgcgcgg ggagcttccc gtcgatcagg taagcctcca cgtgccggtt
     3241 gacgcaggcg ttcggcccgc ccgccagccc gtgcgtgccc gcgccccgct cggtcaccag
     3301 cgcggcgtcc ggctgccgcc gccgcagctc cagcgcgccc gcgtacgggg tcgccgcgtc
     3361 ccgttccccg gcgaggatca gcgtcggcgg cagctcaccg ggggccaccc gcacgtccat
     3421 cggcgtctgc cggggctcct tccagtacgc gcacggcagg ttcatccagg cgttctccca
     3481 ggtctcgaac ggcgccgtgc gggcgagccg ggtgtggtcg cggtcccagg tctgccagct
     3541 ggtgggccac ggcgcgtcgt tgcactccac cgccgtgtag acggcccgcg cgttctccgc
     3601 ctccacccgg ccctcctcca gcggcgtcgc cagattgacc agcggctgcg gatcaccctt
     3661 caggtacgcc gcgagcgcct ccgcgtgcga gggccagtag tcctcgtagt actgggcccg
     3721 caacatcacc gcctggagct gcgccgggcc gacggtgccc ccggcgggct cggccgccag
     3781 cttctcgcgg gcccgccggt accccgccag cacctgggcc tcgtcggtgc ccatcccgta
     3841 cgccgcgtcg tgcgaggcga tccaccgccg gaagtccgcc caccgcttct cgaacgccgc
     3901 cgactgcgcc atgttgttcc ggtaccagac acgcgagggc gaggggtcga ccgccgagtc
     3961 gaagaccatc ctgcgcacgt gcgaggggaa gagcttcgcg tacagcgccc cgaagtacga
     4021 gccgtacgac gcgcccatga acgtcagctt ccgctcaccg aggccggccc gcaccacgtc
     4081 caggtcgcgg gcgttgttca gcgagtggat gtgccgcagc cgcttcccgc cgcgctcggc
     4141 gcacccccgc gcgtacgcct cggcctcggc cttccgcccg gccttctgct tctcggtcgg
     4201 gtgctcgggc gcctgccagt tcccgtccgg cttctcgcgc gggtcccggc aggagagcgg
     4261 cgccgaccgg ccgaccccgc gcggcgcgta cccgaccagg tcgtagaccg aggccagccg
     4321 cttccactcg gggaagatcc ccaccagcgg gaagagcatc ccggaggagc cgggaccgcc
     4381 cgggttgaag accagcgcgc cctgccgctc gttcttcggg cccgccgctt tggcccggct
     4441 caccgtcagg tcgatctgct cgccgtccgg ccgcgcgtag tccagcggga cccgtaccgt
     4501 gccgcactcc agggagcggg gcagcccctc ctcgtccggg cacggaccga aggcgatcgg
     4561 ggtccgcgcc gcccgctcgg ccgccagcgc caccccgcgc gcctcggggc cgccgggcgg
     4621 agccggagcc ccggccgcgg gcgcctgcgg ggaagccgcg agcggcgtga ggagcagcgc
     4681 tccggcggcg gtgaggatga gcggcgcgcg catcgcgtcc ctttcgtccg gccgatgaca
     4741 gccctagggc gtgtccgcaa agtcccgcac ggcgtccacg gcccccggca cgcactctcg
     4801 ccccaccggg cgaaagcccg agtacgtcca gtacgagggc ttccgcccgg cgtgccgaga
     4861 gcacgcaccg gacaccgcgg acaccgcaca ggactttgcg gacacgccct agggatgatt
     4921 cgcaggccgg tcggcagggg caagcaccgg ggcgcgtgtt ggacggaccc gctcggatcg
     4981 gcccaacacg gccgccgggt tcagcgcccc agcagggcga agcgcgccac cctggcccac
     5041 cgggcccagg gcctgcccac cagggaggcg cgccccgagc gctcgatcca ccagtcgcgc
     5101 agggcacgcc ggaccgcccg ctcggccggc ggctcctccc gcaaggtgtg cagcgcgaag
     5161 gcgagcgcgt ccgcgcggta cccgccggtc agcggccggc ccgccgcgta ccgcaggaag
     5221 cccggccggt acgtgtcgcc gaggatctgc tccagctccg gggcgaggga ggccaccacg
     5281 gagccccgct tggcggtcag catccggctc tgcacccgca gccgcccccg gtcgaacccc
     5341 tccggcgccg gcgtccccgc caccagcgcc gacagcagcg ccgtctgtcc gagggccagc
     5401 cgcgtccgcg ccgcctcgtc cgccaccggg ccggtcgccg ccccggcccc cgacgccggt
     5461 acggacccgg gccgggcccc cgcccccacc gggacccggt cccggccggc caccgccacc
     5521 gtccgccgga tcttcgcgac ctccgcctcc agctcggccg ccgacgggaa ccgctcgtcc
     5581 cgctccagca gcaccccggg cggccgcacc cgcgaggcca gctcggccag gaccgccagc
     5641 accggctccg gcaccgggtg ggcgtggctg tcgtgccaca ccccgtcccg ctcgaacccg
     5701 cccgccacat ggacgtacgc cagcgcctcc agcggcagcg cgtcgagggt ggcggcgggg
     5761 tcctcgccct ggttgacgtg gttggtgtgc aggttggcga cgtcgatcag caggcgcacc
     5821 ccggtccggt cggccagctc gtagaggaac tgcccctccg tcagctcctc gcccggccag
     5881 gagaagagcg ccgcgatgtt ctccacggcg agcggcacgg gcagctggtc ctgggcgatc
     5941 cgcacgttct cgcagagcac cgcgagccct tcccgggtcc gggggaccgg caggaggtgc
     6001 ccggcctcca gcccgcgcga cgcggtccgc tccccgcccg cccggacgaa cgcgatgtgc
     6061 tcggtgacca gcggcgaccc cagcgcctcc gcccgctcgg ccagcgccgc cagccgcacc
     6121 ggatcgggcc gctccgcgcc gcccagcccc agccccaccc cgtgcggcac cacggcgacc
     6181 ccacgttccc gcaaccgccg caaggagtcg gggaggtgac cgggacagac gttctccgcc
     6241 accacctcca cccactcgat ccccgccatc gcctcgacgg cctccgcgat cgcgggccgc
     6301 cagccgatcc ccgttcccag ccgttccata ccgccacccc ctccaggaac cccccggtcc
     6361 ctgcggccac cctgcccgcc gccggccgcc ccggaaccgt cacccgccct cttcagagca
     6421 ggatttgagc ttcgccccct caccccacca acgcgggatg ctccgccacc accgtgcacg
     6481 acccgggcgc gatctccgtg aacccggcgt cccgcaccac cggcagcccg ctctccacca
     6541 gctccttcca gcgctccgcg ccggcggtcc gcaccgccag cgggaaaccc tgctcgcgcc
     6601 aggcgtcccg ttccgccccc gacagctgcc accaggccag ctgcgccccg tggcccgcct
     6661 gcgccatcgc cttccccgcc gacatgggca cgtccgggtt gagccagagc accgggacgc
     6721 cgggcgcggg ctcgcccggc tcctcggggt cctccagctc ggtgccggag acctggagct
     6781 tggccagctc cctgggccag ccgtccagcg gcaccggcgg gtagacgcgc acctcggcgc
     6841 cgccgtcctc gaccgtgatc cccggcaggg ccgcggcctt gcgccactcc gcgccccggg
     6901 cccgccgtac caccttgcgg atccgcgcgt cctgccagtc ccgcaccgcc tccgcccaca
     6961 cgccgtcccc ctcggagcgg ggatcgctga gcagcgtcag gacggcccgg gcggccgtct
     7021 ggagcgcgtc ggtgagggcg ggcggctcgg cgcgctccag ccggacgacg agcggcagca
     7081 cgaactgcgg tgcctcgtcg cgggcggtgc gctcgtcacg gaagggactg tcggagaggg
     7141 gttcgctggt cacggagcca gtctgccaaa ccgcgggcga ccctcacccc ttccgcgagg
     7201 ccgccccgat cagctcggac accttcacga agcggtaccc ctgctcgcgc agggcgggca
     7261 cgatccggcg cagcgcgcgc tcggtggtgg gggcggcact gcgggtgcag tgcatgacca
     7321 cgaccgagcc ggggcggacc ccgtccagca cctgctgcgc caccgcgtcg gcgtcggtgg
     7381 cgaaggcgtc cccgctcacc acgtcccact gcacggccgt caccccggcc gcggagacct
     7441 cccgcagcgc cgcgtcgtcg taacacccgc cggggaagcg gaagtacggc atggccttct
     7501 tcaccccggc cgtgcggaac gcggtgtacg cccgttccac gtcggcccgc atctcgctct
     7561 cggcgacgcg cggcaggccg tagcagtcac cggtgaaggc gtggtggctg taggagtggt
     7621 tggcgatctc gaagagcggg tcggcgccga tggcccgtgc ctggtccggg tactcctcgg
     7681 cccaccgccc ggtcatgaag acggtggcgg gcaccttcag gcggcgcagc gaggcgatca
     7741 gctccgggtg gtcgaagcgc tcgccgccgg ccgcgcgctc gccctggtcg gccgtcatgt
     7801 cggcgtcgaa ggtgagcgcg acggtcttgc cgtcgttccg gggcccgttg gcgaagaccg
     7861 gggcgagccc gccggggccg ggcgccagcg tgggcggcgc cgtcggggcg gcgggggagg
     7921 cggagtgcga ggcccgtggc cccggcgccg tgtcatgggg gtccggaacg gactgaccgc
     7981 acccggccac cgcgcccaac gccatcaggg cgcccagcgc cgtaccggtc cggagcttcg
     8041 ccttgcgcac catgctgatc acgtcgtgac cctaggatgc gaaaagctga atatcgggca
     8101 aaacgccgcc ggggcgcgcg gcctcacccg tctcgccccg ccaccctccg ccgtccgggc
     8161 gcccggtggc cgaccggggt cagtcctggc cggtcgctcc gaagccggtg acgacggcga
     8221 aggcgtcggc ggtcaccgat tccagggtca ggctctcgtc gccgcacgcc cagtgctcca
     8281 ccgccgtcgt cgcgacgttg atgagcagcc ccgccgtgac ggccggcgcc agcgcgtccg
     8341 cgggggtgcc cgtccgctcg gccaccagcg gggcgagcag cccctgcgcc cgccgtcccg
     8401 cggcgagcca gcgcgccttg agggcgggga cgccgagcat cacccgggcg gtgcgccggg
     8461 tctggctgcc ggaccggcgg caggagtgca ggagtgcggc gcgggcggcg gtcgtcagcg
     8521 gctcgccggc cggccgcctg gccagctcgg ccacgagcgc gtccacccct tccgccagca
     8581 gcggggtcag ggcctcctcc ttgaccgggc agtgccggta gaaggtgcgc agcgataccc
     8641 ccgtcgcctc ggcgatctgc tccaccgtgg tgccggcgaa gccctgccgc tcgaagaggt
     8701 ccacggcgtg caacgcgatc tcccgcctca ccgagtccct gcgccggtcc cgcaggtccg
     8761 gctgggccgc accgttgtcg ctcaccaaag tgccacgtcc tctcctcgcg ccggaaagtc
     8821 tctcatcctc ccgcccgccg gaacggcaca gctggccgtc atggcacatt gtgccaagta
     8881 ggcataccct ggcatggagg cgccggactc ggcggctcac gcacaccagc aagcgaggaa
     8941 gtggtcacgg tgaccggaat ttcattcgag gggcgcgtcg cgatcgtcac cggcgcgggc
     9001 ggcggactgg ggcgggcgca cgccctcgac ctcgccgccc ggggcgcccg ggtggtcgtc
     9061 aacgacctcg gcggggacat ctcgggcagc ggcggcggca cctccatggc cgaccaggtc
     9121 gtccgggaga tcgtcgccgc cggcggcgag gccgtcgcca actacgactc cgtggccacc
     9181 ccggagggcg gccgggccat cgtccagcgc gccgtcgacg ccttcggcac ggtggacatc
     9241 ctggtcaaca acgcgggcaa catccgcaac gcgcccttca ccgaactgac cccggaggcg
     9301 atcgaggcac tgctggccgt ccacgtgaag ggcggcttct acgtcacgca gcccgccttc
     9361 gccgtcatgc gcgagaaggg gtacgggcgg atcgtcttca ccgcctcggc cgccggtgtc
     9421 ttcggcagcc ccgagcaggc caactacgcc gccgccaaga gctcggtggt cggcctcgcc
     9481 aatgtggtcg cgctggaggg cgccccgcac ggcatcaagg tcaacacgat cctgcccgtc
     9541 tccgagagcc gcatgatcgc ggacatgaac ccggagacgc tcgccggagt accgcggacc
     9601 cccgcccacc gcgagccgga gatgatcacc gccatggtca gctacctcgc gagcgaggcc
     9661 aacccctaca cccgcgagat gttctcgatc gcccgcggcc gctacgcccg ggtcttcgtc
     9721 ggcgtcgccg agggccacca agtgccgtac gaggccccgg tcgccacggc cgacgacgtc
     9781 gccgcgcaca tcgaccgcat ccgtgaccag gagggatacc acgtccccgg ctcgatggtg
     9841 gaggagttct cgctcgtccc ctgagggccg gacgcccccc ggaggggggg agggggaggg
     9901 ggaggggcag gtccggccct gcccctcccc gtcacctcac cggtgccagg gcccggtgac
     9961 cgcgaaggtc gtcccgggcg tgtagcagtt gacgaacatc gtgtcgtagt ccggggagaa
    10021 gcagacgccc gcgaactcac cccacgccgg ctcctcgggc gtgccgatgt tctggcggcc
    10081 ccgtgccatc gggtagacct cgccgcgcgt ggtcacgccg tagacgtgct gggcgccctc
    10141 gccgtcctcg gcgaccatga ggccgccgct cggggcgagg cagatgttgt ccggctcctc
    10201 gcccgggagc tgcacgtcgg tgtcggggcc gaagacgatg accagggtga ggcgccgccg
    10261 gtccgggtcg tagcgccaga tctgcccgta gtggtcggcg gccgagccct cgctgctccg
    10321 ggcgaagctg gagacgaagt agaccgagcg gccgccccag tagcagccct ccagcttctg
    10381 cgcgtgggtg atgcccttcg ggccgaagtc ctggaagcgg atcggcgtct cctcggcgag
    10441 cgggtcgggc acgtccaccc actccacccg gtcgaacgag gcgcccgtct cctggatcgc
    10501 cgagaggtcg ggcaccccgg gcacccgcat cgcctggagc gcgcccccgg cccgcagcga
    10561 gccgaggccg ccgcgcggct tctcgggcag gaagcggtag aagagcccga acggccgctc
    10621 gaaggcgtcc tcggtctcgt agacgatccc gtggcgcggg tcgatcgcga tggcctcgtg
    10681 ctggaagcgg cccatcgcct tcagtggcac cgcgccggtc cggtgcgggt cgaccgggtc
    10741 gacctcgaag atgaagccgt ggtccttggt gtaaccgttg gtcccggcct tgtcctcggt
    10801 ctcctcgcag gtcagccagg tcccccaggg ggtcggcccg ccggcgcagt tggtcgaggt
    10861 cccggcgatg gcgacccgct cgccgagaac ccggtgcccg gggtcgaggg tgagcgccgt
    10921 acagccgccc atcccctccg ggtcgtacgt cagtccgcgt acgggcggga cgcggatcgg
    10981 ggcggagggc cggttctcgt ggttgcggac gaggtggacc tggccccggc gcccgccgaa
    11041 ggcggacatc ccgtcgtggt tgctcggcac ctggccctcg ccggagagca tccggtcgcc
    11101 ctcccgggac agcacccggt agcggaaacc tttcggcagg tccagcagac ccttcgggtc
    11161 gggcaccagc ggaccgtacc cggccttccc catccccttc gcggcggccg tgcccgcgaa
    11221 gagttcggag agactgccgg tgaaggcgat accggcgacg gcaccggtgc gggcgaggac
    11281 ttgacggcgt gtcgcggaca tgggcaactc cctgctgcgg ctctgcgga
//
