LOCUS       NZ_CP047147            10399 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. GF20 chromosome, complete genome.
ACCESSION   NZ_CP047147
VERSION     NZ_CP047147.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GF20
  ORGANISM  Streptomyces sp. GF20
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7311137)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7311137)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: SPAdes v. Nov-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:38:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,372
            CDSs (total)                      :: 6,281
            Genes (coding)                    :: 6,197
            CDSs (with protein)               :: 6,197
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 50 of 84
            Pseudo Genes (internal stop)      :: 9 of 84
            Pseudo Genes (multiple problems)  :: 17 of 84
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:01:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1332835
            Orig. end                         :: 1343234
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047147.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="7"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(107..122)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.95"
                     /tool="antismash"
     misc_feature    complement(265..282)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.25"
                     /tool="antismash"
     gene            complement(294..1385)
                     /locus_tag="GR131_RS05280"
                     /old_locus_tag="GR131_05280"
     CDS             complement(294..1385)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947418.1"
                     /locus_tag="GR131_RS05280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_05280"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_129829183.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3.4e-16, bitscore:
                     57.9, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEDMVLHVKGRILVGPEEARDELWVVGGRITFTRPPGAREVRTV
                     EGWALPGLVDAHCHVGLDRHGPVAAEVAEKQALTDRDAGALLLRDAGSPSDTRWMDDRD
                     DLPRVIRAGRHIARTRRYIRNYAHEIESDQLVAYVGQEARRGDGWVKLVGDWIDRGAGD
                     LTPCWPREAVEAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCIEHATGLTEETIPLF
                     AERGVAIVPTLVNIATFPDLAAGGQAKFPDWSAHMRRLYERRYDTVRAAWDAGIPVFVG
                     TDAGGSLPHGLVAAEVAELVTAGIPPLDALSATAWGARDWLGRPGLTEGAPGDLVVYAS
                     DPRADVRVLAAPRRVVVGGKVVA"
     gene            1460..2575
                     /locus_tag="GR131_RS05285"
                     /old_locus_tag="GR131_05285"
     CDS             1460..2575
                     /NRPS_PKS="Domain: Aminotran_5 (74-284). E-value: 2.7e-30.
                     Score: 97.5. Matches aSDomain:
                     nrpspksdomains_GR131_RS05285_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947419.1"
                     /locus_tag="GR131_RS05285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_05285"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_129829182.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.4e-23, bitscore:
                     80.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLTPLTAAHFANIERRVAALLSTAQDVVITQGEALLPLE
                     GCIRGVAGPDTTALNVITGPYGQTFGDWLRDGGARVVDLAVPYDAAVGADQVAEALAEH
                     PEISFVSLVHAEAATGNTNPVAEIGRVVREHGALFMVDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWQRMAANPRAPRRSYLSLLDWKERWIDAGRTALLHAP
                     AQLEMVALQACVERIEDEGLETVIGRHTRAAAATRAGVKALGGGFAPYVREARHAAPVA
                     TTLRAPEGVDASELVARALAADPVLPLVAGGGALAKEMIRVNHYGTAAAGSTVHSCLSA
                     LGAALTDLTGRSVDPDAARRAATAAWERPAG"
     aSDomain        1682..2311
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR131_RS05285_Aminotran_5.1"
                     /evalue="2.70E-30"
                     /label="GR131_RS05285_Aminotran_5.1"
                     /locus_tag="GR131_RS05285"
                     /protein_end="284"
                     /protein_start="74"
                     /score="97.5"
                     /tool="antismash"
                     /translation="RDGGARVVDLAVPYDAAVGADQVAEALAEHPEISFVSLVHAEAAT
                     GNTNPVAEIGRVVREHGALFMVDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWQRMAANPRAPRRSYLSLLDWKERWIDAGRTALLHAPAQLEMVALQACVERI
                     EDEGLETVIGRHTRAAAATRAGVKALGGGFAPYVREARHAAPVATTL"
     misc_feature    2697..2712
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.17"
                     /tool="antismash"
     misc_feature    complement(2712..2728)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    2715..2728
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.78"
                     /tool="antismash"
     misc_feature    2835..2850
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     misc_feature    complement(2846..2861)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2856..2871
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2895..2913
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 21.64"
                     /tool="antismash"
     misc_feature    2968..2978
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.79"
                     /tool="antismash"
     misc_feature    2990..3002
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    3016..3028
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3069..3575
                     /gene="ectA"
                     /locus_tag="GR131_RS05290"
                     /old_locus_tag="GR131_05290"
     CDS             3069..3575
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412174.1"
                     /locus_tag="GR131_RS05290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_05290"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_008412174.1"
                     /transl_table=11
                     /translation="MKEMPEDLRLDHPGVEDGAAIWRIARDSRTLDLNSSYSYLLWCRD
                     FAGTSVVARDAAGEPVGFVTGYFRPERPHTLVVWQVAVDASQRGRGLAAALLDGLTDLL
                     REKGRLDGIETTITPGNTPSERLFTSYAKRHGAELEREVLFGAGLFPDGGHDPEVLYRI
                     GPFTD"
     gene            3695..4966
                     /gene="ectB"
                     /locus_tag="GR131_RS05295"
                     /old_locus_tag="GR131_05295"
     CDS             3695..4966
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 1.8e-75.
                     Score: 246.0. Matches aSDomain:
                     nrpspksdomains_GR131_RS05295_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="GR131_RS05295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_05295"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_018470508.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.4e-80, bitscore:
                     266.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRSWPAVFDRAQGSYLYDEDGHTY
                     LDFFAGAGALNYGHNNPVLKRALIDYIERDGITHGLDMGTTAKRAFLETFQNVLLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVQDFLWFERLLEDQGSGLNTPAAVIVETIQGEGGINVARP
                     EWLRALADLCKRRDMLLIVDDIQMGCGRTGPFFSFEEAGITPDIVTLSKSIGGYGMPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWSDGQMEKQTLARGEQVEQALT
                     AILDEHGGEAIVSVRGRGLVWGMEFADKSRAGAVSKRAFELGLLVETSGPESEVVKLLP
                     PLTVSPDELDEGLRILARAVRETA"
     aSDomain        3779..4792
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR131_RS05295_Aminotran_3.1"
                     /evalue="1.80E-75"
                     /label="GR131_RS05295_Aminotran_3.1"
                     /locus_tag="GR131_RS05295"
                     /protein_end="366"
                     /protein_start="28"
                     /score="246.0"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGHTYLDFFAGAGALNYGHNNPVLKRALIDYIE
                     RDGITHGLDMGTTAKRAFLETFQNVLLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVQDFLWFER
                     LLEDQGSGLNTPAAVIVETIQGEGGINVARPEWLRALADLCKRRDMLLIVDDIQMGCGR
                     TGPFFSFEEAGITPDIVTLSKSIGGYGMPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWSDGQMEKQTLARGEQVEQALTAILDEHGGEAIVSVRGRGLVWGMEFA"
     gene            5001..5399
                     /locus_tag="GR131_RS05300"
                     /old_locus_tag="GR131_05300"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947422.1"
                     /locus_tag="GR131_RS05300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_05300"
                     /product="ectoine synthase"
                     /protein_id="WP_003947422.1"
                     /sec_met_domain="ectoine_synt (E-value: 1e-54, bitscore:
                     182.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHVKSASGTWESKRIVLAKEKVGFSLHETILY
                     AGTETDMWYANHIEAVLCVEGEAELTNRETGETHWIEPGTMYLLNGHEKHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEED"
     gene            5403..6299
                     /gene="thpD"
                     /locus_tag="GR131_RS05305"
                     /old_locus_tag="GR131_05305"
     CDS             5403..6299
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010637026.1"
                     /locus_tag="GR131_RS05305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_05305"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_010637026.1"
                     /sec_met_domain="PhyH (E-value: 3.8e-37, bitscore: 127.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVASERTDLYPSRTATEVTIPRKDPVVWSPQDAPGPITRSDLQS
                     YEDNGFLAVEELITPEEVGVYRSELDRLVADPLIRADERAIVEPKSEKVRSVFEIHKIS
                     EIFAGLVRDERLLGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPR
                     MRAVSVSIALTENYDTNGGLMIMPGSHRTYVGCNGVTPKDNYKRSLQMQDAGIPSDEVL
                     TEFADRHGIKLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVDNAAEEPFAA
                     PVRRPEFIGARDFTPVR"
     gene            complement(6473..7534)
                     /locus_tag="GR131_RS05310"
                     /old_locus_tag="GR131_05310"
     CDS             complement(6473..7534)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947424.1"
                     /locus_tag="GR131_RS05310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_05310"
                     /product="alkene reductase"
                     /protein_id="WP_010637028.1"
                     /transl_table=11
                     /translation="MSTAFEPLDLSGTKLPHRIAMAPMTRSRSGAAGVPTALMAEYYAQ
                     RASAALIVTEGIQPSAVGQGYPDTPGLHSAEQVTGWRAVTSAVHAAGGRIFAQLMHAGR
                     IGHPSLLPEGEVNVAPSAVAATGQVYTAEGPQDFVTPRELTDEEIRATLADYVTAARNA
                     VDAGFDGVELHGANGYLIHQFLAPGSNRRTDGWGGSVEGRIRFAAEVTEAVVAEIGAAR
                     TALRLSPGNAFNSIEEPEPEPEYTALVARIAPLGLAYLHLAEDGPARRALTLRLRELFD
                     GPLMLNPADSPTGPEALALVEEGTADLVSYGQLFLANPDLPARLRAGGPFNEARRATMY
                     GGGAEGYTDYPAL"
     gene            7705..8217
                     /locus_tag="GR131_RS05315"
                     /old_locus_tag="GR131_05315"
     CDS             7705..8217
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470511.1"
                     /locus_tag="GR131_RS05315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_05315"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_018470511.1"
                     /transl_table=11
                     /translation="MNEATGPEQAPCTWPAERGAAPPGGPVGHLVTRVARLHRTAAGRA
                     LRESGLYPGQEIVLGHLWEADGPVRQSDLIKAVELDPSTVTKTLQRLEQSGHVTRAPDP
                     ADRRAVLVEATELSCALSRQVESAWAGLEEHALAGLTPADRAELLRLLTAVEANLCDRA
                     GGCPEAE"
     gene            complement(8228..9277)
                     /locus_tag="GR131_RS05320"
                     /old_locus_tag="GR131_05320"
     CDS             complement(8228..9277)
                     /NRPS_PKS="Domain: Aminotran_5 (55-343). E-value: 1.9e-22.
                     Score: 71.6. Matches aSDomain:
                     nrpspksdomains_GR131_RS05320_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018895195.1"
                     /locus_tag="GR131_RS05320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_05320"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_159312020.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.7e-17, bitscore:
                     60.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLARSLAEDEFKPSRTHLATAGTGLLPLSAARAVHEAVDAAAAG
                     TPPDMFGARDAARASFARLVGVPATRVATGSSVATFTGLIAAALPDGAEVLTAEGDFSS
                     LVNPFHVRPGVSVRTVPLERIAEHVTGTTALVAVSAVQSADGRRADLAAVREAARDHGA
                     RTLVDASQAAGWDTFEAGAYDYTVAVGFKWLLAPKGTAFLTVPPEPGALPPLLAGWVAG
                     DSPFDSCYGPVTGLAPDARRYDESPAPLVWTGAQHALALLERIGTEAVGAHDVALADRF
                     RERLTRAGHAPVPAPGSAIVSVPGLGERAAALVERGITVSDRAGMLRAAFHLYNTESDV
                     DRLVAALDG"
     aSDomain        complement(8249..9112)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GR131_RS05320_Aminotran_5.1"
                     /evalue="1.90E-22"
                     /label="GR131_RS05320_Aminotran_5.1"
                     /locus_tag="GR131_RS05320"
                     /protein_end="343"
                     /protein_start="55"
                     /score="71.6"
                     /tool="antismash"
                     /translation="AARASFARLVGVPATRVATGSSVATFTGLIAAALPDGAEVLTAEG
                     DFSSLVNPFHVRPGVSVRTVPLERIAEHVTGTTALVAVSAVQSADGRRADLAAVREAAR
                     DHGARTLVDASQAAGWDTFEAGAYDYTVAVGFKWLLAPKGTAFLTVPPEPGALPPLLAG
                     WVAGDSPFDSCYGPVTGLAPDARRYDESPAPLVWTGAQHALALLERIGTEAVGAHDVAL
                     ADRFRERLTRAGHAPVPAPGSAIVSVPGLGERAAALVERGITVSDRAGMLRAAFHLYNT
                     ESDVDRL"
     gene            complement(9403..10128)
                     /locus_tag="GR131_RS05325"
                     /old_locus_tag="GR131_05325"
     CDS             complement(9403..10128)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947427.1"
                     /locus_tag="GR131_RS05325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_05325"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_093846403.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFDKALAAFPHRDRIEVVHRSFELDP
                     ARPKGDPELVLPMLAKKYGMSEAQAAEAERNLGQNAAAEGLPYLTEGRDHGSTFDMHRL
                     LHLAKARGRQDELLAVLYRANFAETATVFGDDERLVALAVEAGLEEAEAREVLADAQRY
                     AAEVRADERAATELGASGVPFFVLDRRYGVSGAQPADVFTQALTQAWESHVPASGLATP
                     AGAEGGEACGPDGCEVPKN"
ORIGIN
        1 ccgcacctgg ggacaggcgg ccgccgacga cgtcgttgcc ggaatcttta cgcactccgg
       61 gtgaaggggc gacaacctgg ggtgacttca ctccaaaggg tggttttggt gcagacgttc
      121 gacccggcct gcacggccgt tcccccacgt cacccctgac cgccgtttcc gggcccccga
      181 agggcgcaga agggcgtacg gacggcgtac gcgggcgcgg acgtgcggcg ggggccggag
      241 cacaacgacg ctgctccggc ccccgtaacg gcccgtcaac acgcttctcg cggtcaggcg
      301 acgaccttgc cgccgaccac gacccggcgg ggcgccgcca gcacccggac gtcggcgcgc
      361 gggtcgctcg cgtagaccac caggtcgccc ggggcaccct cggtcagtcc ggggcggccg
      421 agccagtcgc gggcgcccca cgccgtggcg gagagggcgt cgagcggcgg gatgccggcg
      481 gtgaccagtt cggcgacctc ggcggcgacc aggccgtgcg ggaggctgcc gcccgcgtcg
      541 gtgccgacga agaccgggat gcccgcgtcc caggcggcgc ggaccgtgtc gtaccggcgc
      601 tcgtacagcc gccgcatgtg ggccgaccag tcggggaact tcgcctggcc gccggccgcg
      661 aggtcgggga aggtggcgat gttgaccagg gtggggacga tggcgacgcc gcgttcggcg
      721 aagagcggga tggtctcctc ggtgagcccg gtggcgtgct cgatgcagtc gatgcccgcc
      781 tcgaccaggt cgcgcagcga gtcctcggcg aagcagtggg cggtgacccg ggcgccgagc
      841 cggtgcgcct cggcgatggc ggcctcgacc gcctcgcgcg gccagcacgg cgtcaggtcg
      901 cccgcgcccc ggtcgatcca gtcgccgacc agcttgaccc agccgtcccc gcgccgcgcc
      961 tcctggccga cgtaggcgac gagttggtcc gactcgatct cgtgggcgta gttgcggatg
     1021 tagcggcggg tgcgcgcgat gtggcggccg gcccggatga cgcggggcag gtcgtcgcgg
     1081 tcgtccatcc agcgggtgtc gctgggcgaa ccggcgtcgc gcagcagcag ggccccggcg
     1141 tcgcggtcgg tgagcgcctg cttctcggcg acctcggcgg cgaccgggcc gtgccggtcc
     1201 aggccgacat ggcagtgggc gtcgaccagg cccggcagcg cccatccctc gacggtccgc
     1261 acctcgcggg cgcccggcgg ccgggtgaag gtgatccgcc cgcccaccac ccagagttcg
     1321 tccctggcct cctcgggacc gaccagtatc cggcccttca cgtgcagcac catgtcttcg
     1381 ctcatgcacg gcagcgtagg acccggtggg tacgctcgga ccggcagccc gacgatcgac
     1441 cggcgaagag agcaccaccg tgacgcaccc cctcctggac ctgaccccgc tgaccgccgc
     1501 gcatttcgcg aacatcgagc gccgcgtcgc ggcgctgctc tccaccgcgc aggacgtcgt
     1561 catcacccag ggtgaggcgc tgctgccgct ggagggctgc atccgcggcg tggcgggccc
     1621 ggacaccacc gccctcaacg tgatcaccgg cccgtacggg cagaccttcg gcgactggct
     1681 gcgcgacggc ggggcgcgcg tggtcgacct ggcggtgccc tacgacgcgg cggtcggcgc
     1741 cgaccaggtc gccgaggcgc tggcggagca cccggagatc agcttcgtct cgctggtcca
     1801 cgccgaggcc gcgaccggca acaccaaccc ggtggccgag atcggccggg tggtgcgcga
     1861 gcacggcgcg ctcttcatgg tggacgcggt ggcctcggtc ggcgccgagc cgctgctgcc
     1921 cgacgcctgg ggcgtggacc tctgcgtgat cggcgcccag aaggcgatgg gcggcccggc
     1981 cggggtctcg gcggtctcgg tgagcgagcg ggcctggcag cggatggcgg ccaacccgcg
     2041 ggcgccgcgc cgctcctacc tctcgctgct ggactggaag gagcggtgga tcgacgccgg
     2101 gcgcaccgcc ctgctgcacg cccccgccca gctggagatg gtggcgctcc aggcgtgtgt
     2161 ggagcggatc gaggacgagg gcctggagac ggtgatcggc cggcacaccc gggcggcggc
     2221 ggccacccgg gccggcgtga aggcgctcgg cggcggattc gcgccgtacg tccgcgaggc
     2281 gcgccacgcg gcgccggtgg cgaccacgct gcgggcaccg gagggcgtgg acgcctcgga
     2341 gctggtggcg cgggcgctgg cggcggaccc ggtgctgccg ctggtggccg gcggcggggc
     2401 gctggccaag gagatgatcc gggtcaacca ctacggcacg gccgcggccg gctccacggt
     2461 ccactcctgc ctctccgcgc tgggggcggc gctgaccgac ctcacgggcc ggtccgtcga
     2521 cccggacgcg gcgcggcgcg cggcgaccgc ggcctgggag cgccccgcgg gctgatccgg
     2581 ccccgccggc cccggcgggc cgtccggtgc gcggcgcacg ccgtgcgcgg ggcggcccgt
     2641 cgtgctttcc gttcccgggc acagcgaagt ccgcaccgac ttcggcgcgg acagcgaacg
     2701 atattcttaa tcggtagacc tattacaggg aattccgaat tctttcgcac tcaccttccc
     2761 ctattcgtct gcccgctctc cccgaagcta aacgcatcga ttttccggac accgaaaccg
     2821 cgtaattcgg acgcgtcacg cgtgcgttac gcacttgtga ctgactccac acggtgcccg
     2881 tttcgcggct tatatcctgg acaaatcacc atcttcaggc ggtcgttcgc gcaggcgcac
     2941 ccccgcgccc gataacatag aaagcgctgc catgtaaccc cgacccgcga tgcatttttc
     3001 agagtggatc ggtaaattca atttgcatga ccgccgcaca agccgacctt gaacgtggcc
     3061 gatccaccat gaaggaaatg ccagaggatt tgcgcctgga ccacccgggc gtcgaggacg
     3121 gggcagccat ctggcgtatc gcccgcgact cgcgcaccct cgacctcaac tcctcctaca
     3181 gctacctcct gtggtgtcgc gacttcgccg gcacctccgt ggtggcacgc gacgccgccg
     3241 gcgagccggt cgggttcgtg accggatact tccggcccga gcgcccgcac accctcgtcg
     3301 tctggcaggt cgccgtggac gcctcgcagc gcgggcgggg cctcgccgcc gccctgctgg
     3361 acgggctcac cgacctgctc cgtgagaagg ggcggctgga cgggatcgag accaccatca
     3421 ccccgggcaa cacgccctcc gagcgcctct tcacctccta cgccaagcgc cacggcgccg
     3481 agctggagcg cgaggtcctc ttcggtgccg ggctcttccc cgacgggggg cacgaccccg
     3541 aggtgctgta ccgcatcgga ccgttcaccg actgaccagc gcgaacgccc gcacagcaac
     3601 tccaggtccg gccggaaggc aagcccccgg ccgcccttcc gcacgtatcc ctgacttcgc
     3661 cccgcttcac cccccatctc ccaggagagc agtcgtgacc atcaccccgc ccgccctgag
     3721 cgtcttcgag acccttgagt cggaggtacg gagctactgc cgcagctggc ccgccgtctt
     3781 cgaccgggcc cagggcagct acctgtacga cgaggacggc cacacctacc tcgacttctt
     3841 cgccggcgcc ggcgccctca actacggcca caacaacccg gtgctgaaac gcgcgctgat
     3901 cgactacatc gagcgcgacg gcatcaccca cggcctggac atgggcacca ctgccaagcg
     3961 cgcgttcctg gagaccttcc agaacgtgct gctgcgcccg cgtgacctgc cgtacaaggt
     4021 catgttcccc ggcccgacgg gcaccaacgc cgtcgagtcg gcgctgaagc tggcccgcaa
     4081 ggtcaagggc cgcgagtcca tcgtctcctt caccaacgcc ttccacggca tgtcgctggg
     4141 ctcgctcgcc gtgaccggca acgccttcaa gcgcgccggg gccggcatcc cgctggtgca
     4201 cggcacgccg atgccgttcg acaactactt cgacggcacc gtgcaggact tcctctggtt
     4261 cgagcggctg ctggaggacc agggctcggg cctcaacacc cccgccgcgg tcatcgtcga
     4321 gaccatccag ggcgagggcg gcatcaacgt ggcccgcccc gagtggctgc gcgccctcgc
     4381 cgacctgtgc aagcgccgcg acatgctgct gatcgtcgac gacatccaga tgggctgcgg
     4441 ccgcaccggc ccgttcttct ccttcgagga ggcgggcatc accccggaca tcgtcaccct
     4501 gtcgaagtcc atcggcggct acggcatgcc catgtcgctc tgcctgttca agccggaact
     4561 ggacatctgg gagccgggcg agcacaacgg caccttccgg ggcaacaacc cggccttcgt
     4621 caccgccgcc gccgcgctgg acgcgtactg gagcgacggc cagatggaga agcagaccct
     4681 cgcccggggt gagcaggtcg agcaggcgct caccgccatc ctcgacgagc acggcggcga
     4741 ggccatcgtc tccgtccgcg gccggggcct ggtctggggc atggagttcg ccgacaagtc
     4801 ccgcgcgggc gccgtctcca agcgcgcctt cgagctgggt ctgctggtcg agacttccgg
     4861 gccggagagc gaggtggtca agctgctgcc gccgctcacc gtgagcccgg acgagctgga
     4921 cgagggtctg cgcatcctcg cccgcgccgt ccgagagacg gcctgacaca caacgacgga
     4981 agaaagggca cgaccacact gtgatcgtcc gatcgttcaa ggacatcgag aacaccgacc
     5041 ggcacgtcaa gtcggcgtcc ggcacctggg agagcaagcg catcgtgctc gccaaggaga
     5101 aggtcggttt ctcgctgcac gagaccatcc tctacgcggg caccgagacc gacatgtggt
     5161 acgccaacca catcgaggcc gtgctctgcg tcgagggtga ggccgagctg accaaccgcg
     5221 agacgggcga gacccactgg atcgagcccg gcaccatgta cttgctgaac ggccacgaaa
     5281 agcacaccct gaggccgaag acagattttc gctgcgtctg cgtcttcaac cctcctgtga
     5341 ccggacggga ggaccacgac gagaacggcg tctacccgct gctgaccgag gaggactgag
     5401 tcatgactgt cgcgtcggaa cgcaccgatc tctacccgag ccggactgca accgaggtga
     5461 cgatcccgcg caaggacccc gtggtgtggt ccccgcagga cgcccctgga cccattacgc
     5521 ggtccgacct gcagtcgtac gaggacaacg gcttcctcgc cgtcgaggag ctgatcaccc
     5581 ccgaggaggt cggcgtctac cggtcggaac tggaccggct ggtcgccgac ccgctcatcc
     5641 gggccgacga gcgggccatc gtggagccca agtcggagaa ggtccgctcc gtcttcgaga
     5701 tccacaagat cagcgagatc ttcgccggcc tggtccgtga cgagcggctg ctgggccgcg
     5761 cccggcagat cctcggctcg gacgtctacg tccaccagtc ccggatcaac gtgaagcccg
     5821 gcttcggcgc ctccggcttc tactggcact cggacttcga gacctggcac gccgaggacg
     5881 gtctgccgag gatgcgcgcg gtgtccgtct cgatcgcgct gaccgagaac tacgacacca
     5941 acggcggcct catgatcatg ccggggtcgc accgcacgta cgtggggtgc aacggcgtga
     6001 cgccgaagga caactacaag cggtccctcc agatgcagga cgcgggcatc ccctccgacg
     6061 aggtgctgac cgagttcgcc gaccgccacg gcatcaagct cttcacgggc aaggccgggt
     6121 cggccacctg gttcgactgc aacgccatgc acggctccgg ggacaacatc accccgtacc
     6181 cgcgcagcaa cgtgttcatc gtcttcaaca gcgtcgacaa cgctgctgag gagcccttcg
     6241 cggcgccggt gcggcgtccg gagttcatcg gggcgcgtga cttcactccg gtgaggtgaa
     6301 ccggtgacgc cccgcttcac ccaggtggag caggcgcacc ggacgagccg ctgacagcgg
     6361 gctcatcacg caccggcgca cacggcgccc gggccacggg accgggggcg gccacaggcg
     6421 ggccgctccc ggtccggttg cgtttcacgg gccttctccg cccgtgcgcg ggtcagagcg
     6481 ccgggtagtc ggtgtacccc tcggccccgc cgccgtacat cgtggcgcgc cgggcctcgt
     6541 tgaacggccc cccggcacgg agccgcgcgg gcaggtcggg gttggccagg aagagctggc
     6601 cgtagctgac caggtcggcg gtgccctcct cgaccagggc cagcgcctcg ggaccggtcg
     6661 ggctgtcggc cgggttgagc atcagcgggc cgtcgaagag ctcgcgcagg cgcagggtga
     6721 gcgcccggcg ggcggggccg tcctcggcga ggtgcaggta ggcgaggccg agcggggcga
     6781 tccgggcgac cagcgcggtg tactccggct ccggctcggg ctcctcgatg ctgttgaagg
     6841 cgttgccggg cgagaggcgc agggcggtcc tggcggcgcc gatctcggcg acgaccgcct
     6901 cggtgacctc ggcggcgaac cggatgcggc cctcgaccga gccgccccag ccgtcggtgc
     6961 ggcggttgga gccgggggcg aggaactggt ggatcagata cccgttggcg ccgtgcagct
     7021 cgaccccgtc gaacccggcg tcgaccgcgt tgcgggcggc cgtgacgtag tcggcgaggg
     7081 tggcgcggat ctcctcgtcg gtcagctcac gcggggtgac gaagtcctgc ggtccctcgg
     7141 cggtgtacac ctggcccgtg gcggcgaccg ccgaaggggc gacgttgacc tcgccctccg
     7201 gcagcagcga ggggtggccg atccggccgg cgtgcatgag ctgggcgaag atccggccgc
     7261 ccgcggcgtg cacggcggag gtgaccgcgc gccaccccgt cacctgttcg gccgaatgga
     7321 ggccgggggt gtccgggtac ccctggccga cggcggaggg ctggatgccc tcggtgacga
     7381 tcagggcggc cgaggcgcgc tgtgcgtagt actcggccat gagcgccgtc ggcacgcccg
     7441 ccgcgccgga gcggctgcgg gtcatggggg ccatggcgat gcggtgcggg agcttggtgc
     7501 cggacaggtc gaggggctcg aaggcagtgc tcacgggcgg tctccagtgg gggaacgggc
     7561 ggttccgggc agagcggggc ccggccgacg cattgcatgg tcggccaatc aatggggccg
     7621 ggagccactg taacccattg cttggccgac caaaggtata gtcgggcgga gaccgggacg
     7681 aggacgaccc agcgggagac gtggatgaac gaggcgaccg gccccgagca ggccccctgt
     7741 acctggcccg ccgagcgggg cgcggccccg cccggcgggc ccgtcggcca tctcgtcacc
     7801 cgggtggcgc ggctgcaccg gaccgccgcc ggccgggcgc tgcgcgagtc cggtctctac
     7861 ccgggccagg agatcgtcct cggccacctc tgggaggcgg acggcccggt ccgccagtcc
     7921 gacctgatca aggcggtcga actcgacccc tccacggtga ccaagaccct gcagcggctg
     7981 gagcagtccg ggcacgtcac ccgcgccccc gaccccgccg accggcgggc cgtcctggtc
     8041 gaggcgaccg agctgagctg cgccctgagc cgccaggtcg agtccgcctg ggccggcctg
     8101 gaggagcacg ccctggccgg cctcaccccc gccgaccgcg ccgaactgct ccgcctgctg
     8161 acggcggtgg aggccaacct ctgcgaccgg gcgggcggct gccccgaggc ggagtagccc
     8221 gcgcggctca gccgtcgagc gccgccacca gccggtcgac gtcgctctcg gtgttgtaca
     8281 ggtggaaggc ggcccgcagc atcccggccc ggtcggagac ggtgatcccc cgctccacga
     8341 gggccgccgc ccgctccccc agccccggta cggagacgat ggccgagccg ggggccggta
     8401 cgggggcgtg tccggcgcgg gtcagccgct cccggaagcg gtcggcgagc gccacgtcgt
     8461 gggcgccgac ggcctcggtc ccgatccgct ccagcagggc gagggcgtgc tgcgcgccgg
     8521 tccagacgag cggggcgggg ctctcgtcgt agcggcgcgc gtcgggggcc aggccggtga
     8581 cgggtccgta gcagctgtcg aacggggagt cgcccgcgac ccagcccgcg aggagcggag
     8641 gcagggcgcc gggctccggc gggacggtga ggaaggcggt gcccttcggg gcgagcagcc
     8701 acttgaaccc gacggccacc gtgtagtcgt acgcgccggc ctcgaaggtg tcccagccgg
     8761 cggcctggga ggcgtcgacc agggtgcggg cgccgtggtc gcgggccgcc tcgcggacgg
     8821 cggcgaggtc ggcgcggcgg ccgtcggcgg actgcacggc gctgaccgcg accagggcgg
     8881 tcgtcccggt gacgtgctcg gcgatccgct ccagcggcac ggtccgaacg ctcactcccg
     8941 gccggacgtg gaaggggttg accagcgagc tgaagtcgcc ttcggcggtg agcacttcgg
     9001 cgccgtcggg cagcgcggcg gcgatcaggc cggtgaaggt ggccaccgac gatccggtgg
     9061 cgacgcgggt ggcggggacg cccaccagcc gggcgaagga ggcccgggcg gcgtctcggg
     9121 cgccgaacat gtcgggcggg gtgccggccg cggcggcgtc gaccgcctcg tgcacggccc
     9181 gggccgcgct cagcggcagg aggccggtgc cggcggtcgc caggtgcgtc cgggagggct
     9241 tgaactcgtc ttcggccagg ctgcgggcga ggtccatgcg gccactgtgc accctcgcgc
     9301 ggcagccgtc catggtgcgg cgaccgccga accgtccctg gtgggtacgg gtccggcccg
     9361 ccgccccgcc ggagcggatg gcgacgggcc gggcgggcgc ggtcagttct tcgggacctc
     9421 gcagccgtcg gggccgcacg cctcgccgcc ctcggcgccg gccggggtgg ccagcccgga
     9481 ggcggggacg tggctctccc acgcctgggt gagggcctgg gtgaagacgt cggcgggctg
     9541 ggcgccggag acgccgtacc gccggtcgag gacgaagaac gggacgccgc tcgcgcccag
     9601 ttcggtggcg gcgcgctcgt cggcgcggac ctcggcggcg taccgctgtg cgtcggcgag
     9661 gacctcgcgg gcctcggcct cctccagtcc ggcctcgacg gcgagggcga cgagccgctc
     9721 gtcgtcgccg aagacggtcg cggtctcggc gaagttggcg cggtacagca cggcgagcag
     9781 ttcgtcctgg cggccgcgtg ccttggcgag gtggagcagc cggtgcatgt cgaaggtgct
     9841 gccgtggtcg cggccctcgg tgaggtaggg caggccctcg gcggcggcgt tctgcccgag
     9901 gttccgctcg gcctcggcgg cctgggcctc gctcatgccg tacttcttgg cgagcatcgg
     9961 caggaccagc tcggggtcgc ccttggggcg ggccgggtcc agctcgaagg agcggtgcac
    10021 gacctcgatc cggtcgcggt gcgggaaggc ggcgagcgcc ttgtcgaacc gcgcctttcc
    10081 tacgtagcac cagggacagg cgatgtcgga ccagatctcg acgcgcatgg ctgcggttca
    10141 actcctcggc ccggggaccg ggcaggtcgg gggtgccggg agcggtctcc cggggcgtgc
    10201 ggacaccagg gtacggaggg tgcctccgct ctgctgcaac gcgggggcgg accggtgcat
    10261 tccggaccgg gccgggccgg ccctcggccg ccctcaggcc ccgcgttcgg cccgcccgtc
    10321 cacgtgccgg ggcagcccca gcgggttgcc gtcccgcagc tccggcggca gcagcgcctc
    10381 gggggtggtc tggtacgcg
//
