LOCUS       NZ_CP047147            11368 bp    DNA     linear   CON 30-JAN-2024
DEFINITION  Streptomyces sp. GF20 chromosome, complete genome.
ACCESSION   NZ_CP047147
VERSION     NZ_CP047147.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GF20
  ORGANISM  Streptomyces sp. GF20
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7311137)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Multiple polyene macrolides produced by antifungal Streptomyces from
            gut of Macrotermes barneyi facilitates the termite-fungus mutualism
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7311137)
  AUTHORS   Li,J., Ni,J. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2019) State Key Laboratory of Microbial
            Technology, Shandong Unversity, 72 Binhai Road, Qingdao, Shandong
            266237, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: SPAdes v. Nov-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:38:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,372
            CDSs (total)                      :: 6,281
            Genes (coding)                    :: 6,197
            CDSs (with protein)               :: 6,197
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 50 of 84
            Pseudo Genes (internal stop)      :: 9 of 84
            Pseudo Genes (multiple problems)  :: 17 of 84
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:01:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 396457
            Orig. end                         :: 407825
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047147.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Jinfeng Ni.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11368
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11368
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11368
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6368](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6368)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(64..77)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.15"
                     /tool="antismash"
     gene            complement(96..1880)
                     /locus_tag="GR131_RS01055"
                     /old_locus_tag="GR131_01055"
     CDS             complement(96..1880)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470983.1"
                     /locus_tag="GR131_RS01055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_01055"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_159311745.1"
                     /transl_table=11
                     /translation="MTPPTPQEPPAGPPTRKALRSLLPVLAAHRAMTARTCAAALVDQA
                     ALVALVTLAAHTVGTAVIDGRPPSTATAALLLALVAVRALATWREMDLSHDLAYRVLAL
                     LRVRVFDGIARSAPARIAGRRSGDLASTALGDVEALEFFYAHALAQLLATGTVFTASAA
                     LLGTREPWLLALVLPAAALLLALPVLDGRGRAARGARTRAALADLSAETVETVDGLREL
                     LAFGALNRRRARLRAYGRRLARAQRAEQSWEAGAAALRDLLVVAAVIGVVAVAGHSAVE
                     GRLHGAWTPAAMALALGALAPVADAAASLGQAGSLRAAAARVRAAAEAPAGARPPSTPR
                     PLPEGPLGLHLRGVRFGYGSTPVLDGVDLTVRPGETVALVGASGAGKSTCAHLLARYWD
                     PQGGTVTLVPEDGRPPVALRDLADAELRSAVAVVGQEAPLFHGTLAENLLLAAPDAPPD
                     ALDRAVRACGVDVLAAGLPDGLGTAVGERGATLSGGQRARVALARALVAAPRVLVLDET
                     TAHLDHTGDAELAAALAATAPGRATLVIAHRPATVRRADRVAVLEDGRIVEEGTWQELA
                     SAGGALTRLLSREATATA"
     gene            complement(1877..3622)
                     /locus_tag="GR131_RS01060"
                     /old_locus_tag="GR131_01060"
     CDS             complement(1877..3622)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894136.1"
                     /locus_tag="GR131_RS01060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_01060"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_159311746.1"
                     /transl_table=11
                     /translation="MIPPPELRRAARPAALPLAGATALLALVGATHIGQAVLLALVLAR
                     VARGETGGLPALLAAVAAVVLARALLSRAQRLLAVRAGALVRVRLRDDLLAHLGRTGPG
                     ALSGARAGAVRATLVDGVEGVDAYVSRYLPHLMVTCALPPLLLVAVALVEPQAVAALAP
                     ALLLALAGPRLWDRLLARRGREHWDSYEALAADHLEALQGMATLRAAGAVGRTRARLER
                     RSAALHRATVAKMRVSLADTGLTDLAVQGGTVAAVLVACAAATTGSTTATGTYLLLMLA
                     SECFRPVRDLSREWHAGHLGLSAVDGIAALRHTEGGVPDEGTAPAEWATPPEVRFENVH
                     FTHPGRTRPALTGVAFTAPAGRVTALVGPSGAGKSTLLSLLLRQRDPDAGRITVGGLDT
                     AAYRLDALRRGIAVVSQDTYLFRATVADNLRLARPAATPSQLHEAARAAGIHEELTRLP
                     DGYDTVIGERGATLSGGQRQRLALARALLADAPVLLLDEATSAVDERAEARITRELLAA
                     GRGRTCLLVAHRLEAVRHADHIVVLDHGRVDAAGTHPQLLAAGGLYARLVAAGRTVTPM
                     QQEAA"
     gene            complement(3681..4988)
                     /locus_tag="GR131_RS01065"
                     /old_locus_tag="GR131_01065"
     CDS             complement(3681..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508299.1"
                     /locus_tag="GR131_RS01065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_01065"
                     /product="nitroreductase"
                     /protein_id="WP_159311747.1"
                     /transl_table=11
                     /translation="MTPDATLGHALALARSPRTPARATPPPRRPYPWTGTPLALPATAP
                     DPDGPVDLDHLLRHSLRARPGTGGRLRPAASAGALHPVRAHLLIGPGCTLPPGRYAYDA
                     EVHRVFRRGPAPRGAAPGVIAVLTVTAAHTAAHYGHRAWPLLLLDTGHAAAALALAPQG
                     PGVRVSLDIDAAALAAAAGLPPADRWQRIRPGTETEHPLAAVQVTPRRTQRTAPALDRW
                     AALPLPHRTRTPTRPPARPCGTGRLLELLGAATGTEPAWHPVRRPATVTGRALLERRSA
                     DPATLGHPPDGETVARLLHTAATAWPGGPAWCAAVATPAPALLAPGHHTATPYRLARGD
                     ARPSLAHWAAGQLWLADTGAALLAHGCPSDAPASQIRHDHLAAGFAAGTAHAHAAAEGI
                     NARPVGSWQQADLGAALGDTPGHDWVLHGLALGAAR"
     gene            complement(5001..6368)
                     /locus_tag="GR131_RS01070"
                     /old_locus_tag="GR131_01070"
     CDS             complement(5001..6368)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059075.1"
                     /locus_tag="GR131_RS01070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_01070"
                     /product="YcaO-like family protein"
                     /protein_id="WP_159311748.1"
                     /sec_met_domain="YcaO (E-value: 2.7e-43, bitscore: 147.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAALPLDALVDPHCGLVRDVAPVPHPEGAPPRYTAMTAHVADAR
                     RFGAWPADLVSLGTTFGDPAGAWTAAVAEAVERYCGNRLPPPGHPDAPRRATAAQLTAE
                     GHQVYGPDDVPAYAPWQYRRPAFPYAPLTPGTPALWARGTENGRDCWAPVALTHLNWRQ
                     GELRALPRTHHLNYAGIATGQGLADAAERALLEVVERDALELWWHLDGPTRGIDPGSVP
                     GLDADLAGSGLEVSIVEMPSEFAPCVAALVHDARRGVHAAGFACRFDPAEAARKAVLEA
                     VHTWVFTLGAEAADGWVHQAVEAGVLARGLYLDHRADRRYLDDCGEEFAAVRDLGAHVQ
                     AWLDPRLAPLARRFTAPALGTVPVAELAPGSRERLDAALAAGGHRVLTFDLTTEDVAET
                     ALRVARVLVGGLVPNAPAAFGYYGCPRFATAALARGWRTEPPRDPADFTLAPAPHM"
     gene            complement(6365..7891)
                     /locus_tag="GR131_RS01075"
                     /old_locus_tag="GR131_01075"
     CDS             complement(6365..7891)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639446.1"
                     /locus_tag="GR131_RS01075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_01075"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_159311749.1"
                     /transl_table=11
                     /translation="MTPATTRLTTPDGTTLATDLHLPATPGPHPAVLIRTPYDRRAHRA
                     ELRGWAERGFAAVAQDVRGRHGSAGEWHPYRDHEAEDGVATLDWIRAQPWSDGRVVAAG
                     ASYAAYCALAAALCPGARGPDAVIAAVPALGLAETAREPTGPERLWARLGWWAAHGDRR
                     DSAPDALDRALARDPSLPAHLPLTRIADRLDRQLPSWPALWADCRRGRLVAHGSAARLP
                     LLAVGGTRDPFAEDAVALWRGWGGPARLLLGPWGHTLTAETRPGTPGRVDLGALYARWA
                     RAALDGELGPGRRGVIGLAESGRWHHLTDTPTRREGHEVAGSVWQFGAANGLRLLVGAE
                     FTADPDRPVRSDDLTVPRGAPADRCLLATPPLPRPLDLAGPAEARLTVVADTPRADWAV
                     RVVALDPAGRADPLAFGIVRRDAPPGTPHPVTVPLGTLGRTLAAGTRLRVEIAGHHFPA
                     HARNPHTGEDPVTATRLAASRRTVRSEGSALHLPVAGRPRPVDPVPETCR"
     gene            complement(7888..8733)
                     /locus_tag="GR131_RS01080"
                     /old_locus_tag="GR131_01080"
     CDS             complement(7888..8733)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470988.1"
                     /locus_tag="GR131_RS01080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_01080"
                     /product="hypothetical protein"
                     /protein_id="WP_146582355.1"
                     /transl_table=11
                     /translation="MTSSRLAPGAGIVTVPGGRPAVRTAEGEFLHLDTGTADLAALTRH
                     LVGGEAAPDAEVARVAAAFEREGHAVRPGPEEGGALTGRTVLVLGDARLTGPLARYAAA
                     EGARTRHATPEHVGRLADGEGADASTVVVWCLDEPAPPALWDAADRLPAHGTAWLRCHR
                     EGAHLWTEPLAAAPGDVTSAHVRLRRLAATSAHRQLAAYWAGHRTPDNGPHPGEAACAL
                     VAALLTADLLAWAAPGPGSGLPARRRLRRVDLRDLTVTEHPVLPVPPVAPLPGRGSGPA
                     "
     gene            complement(8875..9009)
                     /gene="amiA"
                     /locus_tag="GR131_RS32500"
     CDS             complement(8875..9009)
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508296.1"
                     /locus_tag="GR131_RS32500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_008409986.1"
                     /transl_table=11
                     /translation="MEHDKVFAPIADPGQLAHLSATHSNALVENPFDDADEAGAADDK"
     misc_feature    complement(9075..9089)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 13.03"
                     /tool="antismash"
     misc_feature    complement(9076..9089)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 10.54"
                     /tool="antismash"
     misc_feature    9077..9090
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.02"
                     /tool="antismash"
     misc_feature    complement(9077..9092)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.12"
                     /tool="antismash"
     gene            complement(9078..9914)
                     /locus_tag="GR131_RS01085"
                     /old_locus_tag="GR131_01085"
     CDS             complement(9078..9914)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639452.1"
                     /locus_tag="GR131_RS01085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_01085"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_018894131.1"
                     /transl_table=11
                     /translation="MDHREPGAVVAEGLRFGYPGREVLRGVDLRIGAGELVALVGLNGC
                     GKSTFLRLAAGLLRPDAGRVLLDGEEVARLSRRGAARRVALLHQSAPPVPGLTVRQLVR
                     QGRYAARGPLGMLRDGDDAVSARALADAGVADWAGRPVDALSGGERQRVRLAMALAQDT
                     RILLLDEPTTYLDLRHQLEVLQTVVRLRAERGLTVVMVVHDLNHAARFADRIVALRDGR
                     VAADGKPAAIVTPALLAEVLGVEGRVAPDERGGWPVCYPDHPLGAASHLRNENHIH"
     gene            complement(10037..11092)
                     /locus_tag="GR131_RS01090"
                     /old_locus_tag="GR131_01090"
     CDS             complement(10037..11092)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894130.1"
                     /locus_tag="GR131_RS01090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GR131_01090"
                     /product="iron ABC transporter permease"
                     /protein_id="WP_129846482.1"
                     /transl_table=11
                     /translation="MSAARTAAPVLRRGRTGTRVKAAVLVVLALAAGAAGLVAGPAVTT
                     ADVAEVFAGGGDPLARHIVLNLRLPRALVALTAGACLALAGLVLQSALRNPLAGPEVTG
                     VTPGAVLGAVTATGLGLAGWQSPTAVVVAACLGGCAGAGLLWLLAGRERGDPEQTAVHG
                     VLVSAVLTGATAVVLLIAPGELGSVVQWLVGSTEGRVWRHWELLWPWALCWGAAAWLLA
                     GPLTLLRCGDEQAAALGLDTGRARAAALACAVALTAGAVSAVGALGFVGLLVPHVALAV
                     FGADLRATLPGAALLGAVVVGGADAGAQLLSRALAQALDADRLTVPVGALTTCAGAALL
                     LVVARRRADLR"
     misc_feature    11157..11171
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 15.61"
                     /tool="antismash"
ORIGIN
        1 cagcactcct tcaccggacc gaggagccgg ggtccccacg aggggccccg gctctttcac
       61 gtgtgcgtgc gcgtgcacgc acgcttcagt gcagctcagg cggttgccgt cgcctccctg
      121 gagagcaggc gggtcagcgc gccgccggcg gaggccagct cctgccaggt gccctcctcc
      181 acgatccggc cgtcctccag gacggcgacg cggtcggccc ggcgcacggt cgcgggccgg
      241 tgggcgatga ccagcgtggc gcgcccgggg gccgtggcgg cgagggcggc ggcgagttcc
      301 gcgtccccgg tgtggtccag gtgggccgtc gtctcgtcca ggaccagtac gcggggagcg
      361 gcgaccaggg cccgtgccag ggccactcgg gcccgctgtc cgccggagag ggtggcaccc
      421 cgctccccca cggccgtacc gagaccgtcg gggagtccgg cggcgaggac atcgacgccg
      481 caggcccgga cggcgcggtc cagcgcgtcc ggcggggcgt cgggggcggc cagcagcagg
      541 ttctcggcga gggtgccgtg gaagaggggg gcttcctggc cgacgacggc gacggcgctg
      601 cgcagctcgg cgtcggccag gtcccgcagg gcgaccggtg gccgtccgtc ctcggggacg
      661 agggtgacgg tgcccccctg agggtcccag tagcgggcca gcaggtgcgc gcaggtggat
      721 ttgcccgcgc cggaggcgcc gaccagggcg acggtctcgc ccggacggac ggtgaggtcc
      781 accccgtcca gcacgggtgt ggagccgtac ccgaagcgca cgccacgcag gtggaggccg
      841 aggggtccct cgggcagcgg gcgcggtgtg ctcggcggcc gggcgccggc gggggcttcg
      901 gcggcggcac ggacgcgggc ggcggcggca cgcaggctgc cggcctgccc gagcgaggcg
      961 gcggcgtcgg cgacaggggc gagggcgccg agcgccaggg ccatcgcggc cggggtccag
     1021 gcgccgtgca ggcggccctc gacggcgctg tggcctgcga cggcgacgac gccgatcacg
     1081 gcggccacca ccagcaggtc gcggagggcg gcggcaccgg cctcccagga ctgctcggcc
     1141 cgttgcgcgc gggccagccg ccgcccgtac gcccgcaggc gggcgcgccg gcggttcagc
     1201 gcgccgaagg cgaggagttc gcgcagcccg tcgacggtct ccacggtctc ggcggagagg
     1261 tcggcgaggg ccgcgcgggt gcgggctcct cgtgcggcgc ggccccgtcc gtcgaggacg
     1321 ggcagggcga gcaggagcgc ggcggcgggc aggacgaggg cgagcagcca gggttcgcgg
     1381 gtgcccagca gggccgccga ggcggtgaaa accgtgccgg tggcgaggag ttgggcgagg
     1441 gcgtgggcgt agaagaactc cagcgcctcg acgtcgccca gcgcggtgga ggcgaggtcg
     1501 ccgctgcggc gtccggcgat gcgggcgggg gcgctgcggg cgatcccgtc gaagacgcgg
     1561 acgcgcagga gggcgaggac ccggtaggcg aggtcgtggg agaggtccat ctcgcgccag
     1621 gtggccaggg cgcgcacggc gaccagggca aggagcagcg cggcggtggc ggttgagggg
     1681 ggccgcccgt cgatcacggc cgttccgacg gtgtgggcgg cgagggtgac gagggcgacg
     1741 agggccgcct ggtcgacgag ggcggccgca caggtacggg cggtcatggc gcggtgggcg
     1801 gcgagcacgg gcagcagcga gcgaagggct ttgcgcgtgg gcgggccggc cggtggctcc
     1861 tgcggggtgg gcggggtcat gcggcctcct gctgcatcgg ggtgacggtg cggcccgcgg
     1921 cgaccaggcg ggcgtacagg ccgccggcgg cgaggagttg ggggtgggtc ccggcggcgt
     1981 cgacgcggcc gtggtcgagg acgacgatgt ggtcggcgtg gcggacggct tcgaggcggt
     2041 gggcgacgag caggcaggtg cggccccgtc cggcggccag gagttcccgg gtgatgcgcg
     2101 cctcggcgcg ttcgtccacg gcgctggtgg cctcgtcgag gaggaggacc ggggcgtcgg
     2161 cgagcagggc gcgggccagg gcgaggcgct ggcgctggcc gccggagagg gtggcgccgc
     2221 gttcgccgat caccgtgtcg tacccgtcgg ggagccgggt cagctcctcg tggatgccgg
     2281 cggcgcgggc ggcctcgtgg agctgggacg gggtggcggc ggggcgggcg aggcggaggt
     2341 tgtcggcgac ggtggcgcgg aagaggtagg tgtcctggga gaccacggcg atgccccggc
     2401 ggagcgcgtc cagccggtac gcggcggtgt ccaggccgcc gacggtgatc cgcccggcgt
     2461 cggggtcgcg ctggcggagc aacaggctca gcagggtgga cttcccggcg ccggaggggc
     2521 cgaccagggc ggtgacgcgt ccggccgggg cggtgaaggc gacgcccgtg agggcggggc
     2581 gggtccggcc cgggtgggtg aagtggacgt tctcgaaacg gacttcgggg ggcgtggccc
     2641 attcggcggg ggcggtgccc tcgtcgggga cgccgccctc ggtgtggcgc agcgcggcga
     2701 tgccgtcgac ggccgacagc cccaggtgcc cggcgtgcca ctcgcgggag aggtcgcgga
     2761 ccgggcggaa gcactcggag gccagcatca gcagcaggta ggtgccggtc gcggtggtgc
     2821 tcccggtggt ggctgcggcg caggcgacga ggacggcggc gacggtgccg ccctgcacgg
     2881 cgaggtcggt gaggccggtg tcggcgaggg agacccgcat cttggccacg gtggcccggt
     2941 gcagcgcggc cgaacgccgc tccagccggg cccgggtgcg gccgacggcg cctgcggcgc
     3001 gcagggtcgc catcccctgg agggcctcca ggtggtcggc ggcgagcgcc tcgtaggagt
     3061 cccagtgctc gcggccccgg cgggcgagga gccggtccca gaggcggggc ccggcgagcg
     3121 ccagcaggag ggcgggggcg agtgcggcca cggcctgcgg ctcgacgagg gcgacggcga
     3181 ccagcagcag gggcgggagg gcgcaggtga ccatcaggtg gggcaggtag cgggagacgt
     3241 acgcgtccac gccctcgacg ccgtcgacga gggtggcccg gaccgctccc gcccgggctc
     3301 cgctgagcgc ccccgggccg gtccggccga ggtgggcgag gaggtcgtcg cggaggcgga
     3361 cgcggaccag ggctccggcg cggacggcca gcagccgctg ggcgcggctg agcagcgcgc
     3421 gggccagtac gacggcggcc acggcggcga gcagcgcggg cagcccgccc gtctcgcccc
     3481 gggcgacgcg ggcgaggacg agggcgagca gcaccgcctg cccgatgtgg gtggcgccga
     3541 ccagggcgag cagggcggtg gccccggcca gtggcagggc ggcgggccgg gcggcgcgcc
     3601 gcagttcggg gggcgggatc atcgcgggta cgtctctccg ggcggggtga ggtggtcggg
     3661 ggcgggccgt gggcggcggc tcatcgggcg gctcccagtg ccaggccgtg caggacccag
     3721 tcgtgtcccg gagtgtcgcc cagggcggcg ccgaggtccg cctgttgcca ggagccgacg
     3781 gggcgggcgt tgattccctc ggcggcggcg tgggcgtggg cggtgcccgc ggcgaagccg
     3841 gcggcgaggt ggtcgtggcg aatctgggag gcgggggcgt ccgaggggca gccgtgggcg
     3901 aggagtgccg ctccggtgtc ggcgagccag agctggccgg cggcccagtg ggcgaggctg
     3961 gggcgggcgt cgccccgggc gaggcggtac ggcgtggcgg tgtggtggcc gggggcgagc
     4021 agggcggggg cgggggtggc gacggcggcg caccaggcgg ggccgccggg ccaggcggtg
     4081 gcggcggtgt gcagcaggcg ggccaccgtc tccccgtccg gcggatggcc cagggtggcg
     4141 gggtcggcgc tgcggcgctc cagcagggcg cgtccggtga cggtggcggg gcggcggacg
     4201 gggtgccagg ccggttcggt cccggtggcg gcgcccagca gttccagcag gcggccggtg
     4261 ccgcagggcc gggccggggg ccgggtgggg gtgcgggtgc ggtgcgggag cggcagggcc
     4321 gcccagcggt cgagggcggg ggcggtgcgc tgggtgcggc ggggcgtcac ctggacggcg
     4381 gcgagggggt gttcggtctc ggtgcccggc cgtatccgct gccagcggtc ggccgggggc
     4441 agcccggcgg cggccgcgag cgcggcggcg tcgatgtcga ggctgacgcg gacgccgggt
     4501 ccctgcgggg cgagggcgag ggcggcggcc gcgtggccgg tgtcgaggag cagcaggggc
     4561 caggcgcggt ggccgtagtg ggcggcggtg tgtgcggcgg tgacggtgag gacggcgatc
     4621 acgccggggg ccgctccccg gggggccggg ccgcgccgga acacccggtg gacctcggcg
     4681 tcgtaggcgt accgccccgg gggcagggtg cagccggggc cgatcaggag gtgggcgcgg
     4741 acggggtgga gggcaccggc ggaggcggcg ggccggagtc ggccaccggt gccggggcgg
     4801 gcacgcaggg agtggcgcag caggtggtcg aggtcgacgg ggccgtcggg gtcgggggcg
     4861 gtggcgggga gggcgagcgg ggtgccggtc caggggtagg ggcggcgcgg gggcggggtg
     4921 gcgcgggccg gggtgcgggg tgatctggcg agggcgaggg cgtggccgag ggtggcgtca
     4981 ggggtcatga gggagccggg tcacatgtgc ggggcggggg cgagggtgaa gtcggcgggg
     5041 tcgcgcgggg gttcggtgcg ccagccccgg gcgagggcgg cggtggcgaa gcgggggcag
     5101 ccgtagtagc cgaaggcggc gggggcgttg gggacgaggc cgccgaccag gacgcgggcg
     5161 acgcgcaggg cggtctcggc gacgtcctcg gtggtcaggt cgaaggtgag gacgcggtgg
     5221 ccgcccgcgg cgagggcggc gtcgagccgc tcccggcttc cgggcgccag ctcggcgacg
     5281 gggacggtac cgagggcggg tgcggtgaag cggcgggcga ggggggcgag gcgcgggtcg
     5341 agccatgcct ggacgtgggc gccgaggtcc cgtacggcgg cgaactcctc gccgcagtcg
     5401 tcgaggtagc ggcggtcggc ccggtggtcc aggtagaggc cgcgggccag gacgccggcc
     5461 tcgacggcct ggtgcaccca gccgtcggca gcctcggccc ccagggtgaa gacccaggtg
     5521 tggacggctt ccaggacggc cttgcgggcg gcttcggccg ggtcgaagcg gcaggcgaaa
     5581 ccggccgcgt ggacaccccg ccgggcgtcg tggaccaggg cggccacgca gggcgcgaac
     5641 tccgagggca tctcgacgat gctcacctcc aggccggacc cggcgaggtc ggcgtcgagc
     5701 ccggggacgc tgccagggtc gatgccccgg gtgggcccgt ccaggtgcca ccacagttcg
     5761 agggcgtccc gctcgaccac ttcgaggagg gcgcgttcgg cggcgtcggc gagtccctgt
     5821 ccggtggcga tgcccgcgta gttgaggtgg tgggtgcgcg gcagtgcacg cagttctccc
     5881 tggcgccagt tgaggtgggt gagggcgacc ggcgcccagc agtcacggcc gttctccgtg
     5941 ccgcgcgccc agagggcggg ggtgccgggc gtgagcgggg cgtaggggaa ggcgggacgg
     6001 cggtactgcc agggcgcgta ggcgggcacg tcgtccgggc cgtacacctg gtgtccctcg
     6061 gcggtgagct gggcggcggt ggcgcggcgg ggtgcgtcgg ggtggccggg cggcgggagc
     6121 cggttgccgc agtaccgttc gacggcctcg gcgacggcgg cggtccaggc accggccggg
     6181 tcgccgaagg tggtgccgag cgagaccagg tcggcgggcc aggcgccgaa ccggcgggcg
     6241 tcggcgacgt gcgcggtcat ggcggtgtag cggggtggcg cgccctcggg gtgcgggacg
     6301 ggcgcgacgt cgcggacgag tccgcagtgg gggtcgacga gggcgtcgag gggcagggcg
     6361 gcggtcatcg gcaggtctcc ggtacggggt cgacggggcg ggggcggccg gccacgggca
     6421 ggtggagggc gctgccctcg ctgcggacgg tgcggcggga ggcggccagg cgggtggcgg
     6481 tgacggggtc ctcgccggtg tgcgggttgc gggcgtgcgc ggggaagtgg tggccggcga
     6541 tctcgacgcg cagcctggtc ccggcggcca gggtccgccc gagggtcccg agcggcacgg
     6601 tgacggggtg cggggtgccg ggcggggcgt cgcgccggac gatgccgaag gcgaggggat
     6661 cggcgcgtcc ggccgggtcg agggcgacga cgcgtaccgc ccagtcggcg cggggcgtgt
     6721 ccgcgacgac ggtcagccgg gcctcggcgg ggcccgccag gtcgaggggg cggggcagcg
     6781 gcggggtggc gagcaggcag cggtcggcgg gcgccccgcg cgggacggtg aggtcgtcgg
     6841 agcggacggg gcggtccggg tcggcggtga actcggcgcc gacgagcaga cgcaggccgt
     6901 tcgcggcgcc gaactgccac acggagccag ccacttcatg cccctcgcgc cgggtcgggg
     6961 tgtccgtgag gtggtgccag cgtccgctct ccgcgaggcc gatgacgccg cgccgcccgg
     7021 gaccgagttc tccgtcgagc gcggcccggg cccagcgggc gtagagggcc ccgaggtcga
     7081 cgcggcccgg ggtgcccggg cgggtctccg ccgtgagggt gtggccccac gggccgagca
     7141 gcagccgggc ggggccgccc cagccgcgcc agagggcgac ggcgtcctcg gcgaaggggt
     7201 cgcgggtgcc gccgacggcg agcagcggga gccgggcggc ggagccgtgg gcgaccaggc
     7261 gcccacgccg gcagtccgcc cacagcgcgg gccaggacgg gagttggcgg tcgagccggt
     7321 ccgcgatccg ggtcagcggc aggtgtgcgg gcaggctcgg gtcccgggcg agggcgcggt
     7381 cgagggcgtc gggcgcggag tcgcgccggt cgccgtgggc cgcccaccag ccgagacggg
     7441 cccagaggcg ttcgggtccg gtgggttcgc gggccgtctc ggcgaggccg agggcgggga
     7501 cggcggcgat caccgcgtcc gggcctcggg cgccggggca gagggcggcg gcgagggcgc
     7561 agtacgcggc gtacgaggcc ccggcggcga ccacgcggcc gtcgctccac ggctgggcgc
     7621 gtatccagtc cagcgtggcc acgccgtcct cggcctcgtg gtcgcggtac gggtgccact
     7681 cccccgccga gccgtgccgg ccgcgcacgt cctgcgcgac ggcggcgaac ccgcgctccg
     7741 cccagccgcg cagctcggcc cggtgggcgc ggcggtcgta cggggtgcgg atcagcaccg
     7801 cggggtgggg gcccggcgtg gcggggaggt ggaggtcggt ggcgagggtg gtgccgtcgg
     7861 gggtggtgag gcgggtggtc gcgggcgtca tgccgggccg ctcccccgtc cgggcagcgg
     7921 ggccaccggc ggtacgggca gtacggggtg ttcggtgacg gtgaggtcgc gcaggtcgac
     7981 gcggcggagc ctgcggcgcg cggggagccc ggagccgggg cccggcgcgg cccaggcgag
     8041 gaggtcggcg gtgaggaggg cggcgaccag ggcgcaggcg gcctcgccgg ggtgcgggcc
     8101 gttgtcgggg gtgcggtgtc cggcccagta ggcggcgagc tggcggtggg cggaggtggc
     8161 ggcgaggcgg cggaggcgga cgtgggcgga ggtcacgtcg ccgggggcgg cggcgagcgg
     8221 ttcggtccac aggtgggcgc cctcgcggtg gcagcgcagc caggccgtgc cgtgtgcggg
     8281 cagccggtcg gcggcgtccc agagcgcggg cggcgccggc tcgtcgaggc accagacgac
     8341 gacggtggac gcgtccgccc cttcaccgtc ggcgagccgg ccgacgtgct cgggggtggc
     8401 gtgccgggtg cgggcgcctt cggcggccgc gtaccgggcg agcgggccgg tgagccgtgc
     8461 gtcacccagc accaggacgg tacgaccggt gagcgcgccg ccctcttcag ggcccggccg
     8521 gaccgcgtgg ccctcgcgct cgaacgccgc cgcgacgcgg gccacttcgg cgtcgggggc
     8581 ggcttcgccg ccgaccaggt ggcgggtgag cgcggcgagg tcggcggtgc cggtgtcgag
     8641 gtgcaggaac tcgccctcgg cggtgcgtac ggcgggccgg ccgccgggga cggtgacgat
     8701 cccggcgccc ggggcgagtc ggctgctggt cacggaaacc tccgtcggat gaagtgcgag
     8761 gggatgagcg gcgccgcgct gcggggcgcc gaacgcaacg ggcccgcccg ggggttcgcc
     8821 gggcgggccc gtcacgggtg tcagccggcc gccgtcaccg gcggccgggg ccgactactt
     8881 gtcgtccgcg gcaccggcct cgtcggcgtc gtcgaagggg ttctcgacga gggcgttgga
     8941 gtgggtggcg gagaggtggg ccagctggcc cgggtcggcg atcggggcga acaccttgtc
     9001 gtgctccatg agttgagctc cattcggttc gatgcgtggt ggacgtgaac ggcgtggcgc
     9061 ctgccgcact acggacatta atgaatatga ttttcattcc gcaagtggct tgcggccccc
     9121 agagggtgat ctgggtaaca gaccggccag ccgccccgct cgtccggcgc cacccgcccc
     9181 tcgacaccca gcacttcggc gaggagcgcg ggggtgacga tggcggcggg cttgccgtcg
     9241 gcggccaccc gcccgtcccg gagggcgacg atccggtcgg cgaaccgggc ggcgtggttg
     9301 aggtcgtgca ccaccatcac caccgtcaga ccgcgttccg cgcgcagccg gaccacggtc
     9361 tggagcactt ccagctgatg cctgaggtcc aggtaggtgg tcggttcgtc gaggaggagg
     9421 atccgggtgt cctgggccag cgccatggcc agccgtacgc gctgccgctc cccgccggag
     9481 agcgcgtcga ccggacgccc ggcccagtcg gcgaccccgg cgtcggccag cgcccgcgcg
     9541 ctcaccgcgt cgtccccgtc gcggagcatc ccgagcggcc cccgggccgc gtaccgaccc
     9601 tgccggacca gctggcgcac ggtcagcccc ggtacgggcg gcgccgactg gtggagcagg
     9661 gccacccggc gggcggcgcc ccggcgggag agccgcgcca cctcctcacc gtccagcagg
     9721 acgcgcccgg cgtccggacg gagcagcccg gcggcgagcc gcaggaaggt gctcttcccg
     9781 cagccgttga ggccgaccag ggcgaccagt tcccccgcgc cgatccgcag gtcgaccccg
     9841 cgcagcacct cgcgtcccgg atagccgaac cgcagccctt ccgcgaccac ggcaccgggt
     9901 tcccgatggt ccatcacttc tccttccggc cccgcccgcg ctcgccgggc ggggccgtca
     9961 ctcgtcggta ccggcggcgc ggacgcccgc acggccgtgg ttcggtacgc ctcgggggcg
    10021 tgcggtgtcg gccggctcat cgcaggtcgg cgcggcgccg ggccacgacc agcagcaggg
    10081 cggcgcccgc gcaggtggtc agggcgccca cggggacggt gagccggtcc gcgtccaggg
    10141 cctgagccag ggcgcgggag agcagttggg cccccgcgtc ggcgccgccc accacgaccg
    10201 cgccgagcag ggcggcgccg ggcagagtgg cccgcaggtc ggcgccgaac acggcgaggg
    10261 cgacgtgcgg gaccagcagc ccgacgaagc cgagcgcgcc caccgccgag accgctccgg
    10321 cggtgagtgc caccgcgcag gccagggcgg cggcgcgggc ccgcccggtg tcgaggccca
    10381 gcgcggcggc ctgttcgtcg ccgcatcgca gcagcgtcag cggcccggcc agcagccagg
    10441 cggcggcccc ccagcacagg gcccacggcc acaggagttc ccagtgccgc cagacgcgtc
    10501 cctcggtgga gccgaccagc cactgcacga cgctgccgag ttcgccgggg gcgatcagca
    10561 gcaccacggc ggtggcaccg gtcagcacgg cggagaccag gacgccgtgg acggcggtct
    10621 gttcggggtc gccccgctcc cgcccggcga gcagccagag cagcccggcc ccggcgcagc
    10681 caccgaggca ggcggccacc accacggcgg tcggcgactg ccagccggcc aggccgagcc
    10741 cggtcgcggt gaccgccccg agcaccgccc ccggggtgac cccggtgacc tcgggaccgg
    10801 ccagcgggtt gcgcagggcc gactggagga ccagcccggc cagggcgaga caggccccgg
    10861 cggtgagggc gaccagggcg cgcggcagac ggaggttgag cacgatgtgc cgggcgagcg
    10921 ggtccccgcc gcccgcgaag acctcggcga cgtcggccgt ggtcacggcg ggcccggcga
    10981 cgagccccgc cgccccggcc gcgagggcga ggaccaccag gacggccgcc ttgacccggg
    11041 taccggtccg gcctctccgg agcaccggcg cggcggtccg cgcggcgctc accgctcggc
    11101 cttccgcagg gcggcccggt ggccgggcgc gggggccggt gaaccgcgtt cgggcgtgcg
    11161 ccatttgagc agggccacac cgaccggcac cccgaacagg gcggtccagg cgccgaccgg
    11221 ggtctcgacg ggggcgagca ggagccgtgc cggcaggtcg gcgcagacca cgacaacggc
    11281 gccgcaggcg gccgcgtacc cgagcagccc cgccgcgtcg gcgccgggcc gcagccgccg
    11341 ggccaggtgc ggcgcgagga agccgacc
//
