LOCUS       NZ_BLIS01000009        20873 bp    DNA     linear   CON 26-AUG-2024
DEFINITION  Streptococcus canis strain FU1 ScFU1_contig09, whole genome shotgun
            sequence.
ACCESSION   NZ_BLIS01000009
VERSION     NZ_BLIS01000009.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptococcus canis
  ORGANISM  Streptococcus canis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Yoshida,H., Katayama,Y., Fukushima,Y., Taniyama,D., Mizutani,T. and
            Takahashi,T.
  TITLE     Draft Genome Sequence of Streptococcus canis Clinical Strain FU1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 267874)
  AUTHORS   Yoshida,H., Katayama,Y., Fukushima,Y., Mizutani,T. and Takahashi,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2019) Contact:Haruno Yoshida Kitasato University,
            Kitasato Institute for Life Sciences, Laboratory of Infectious
            Diseases; 5-9-1, Shirokane, Tokyo 108-8641, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 6.5.1
            Genome Coverage                   :: 196x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009811695.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 03:59:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,037
            CDSs (total)                      :: 1,982
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 42 of 98
            Pseudo Genes (incomplete)         :: 70 of 98
            Pseudo Genes (internal stop)      :: 33 of 98
            Pseudo Genes (multiple problems)  :: 38 of 98
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:23:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 43027
            Orig. end                         :: 63900
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BLIS01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20873
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="1"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20873
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20873
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10873](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10873
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            325..807
                     /locus_tag="GVO03_RS04345"
                     /pseudo=""
     CDS             325..807
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044319.1"
                     /locus_tag="GVO03_RS04345"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ferrous iron transport protein A"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIKLSHAKVGIAYQVEGIDLPQGHQKHLAHLGITKGSQVKVMSQT
                     KQSAIVMVKTSRLAFDDSILETIMVSEVIGEESKACPCLN"
     gene            804..>905
                     /locus_tag="GVO03_RS04350"
                     /pseudo=""
     CDS             804..>905
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804687.1"
                     /locus_tag="GVO03_RS04350"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="FeoB small GTPase domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTKIALIGNPNSGKSSLFNLLTRTNQRVGNWPEV"
     gene            1460..2248
                     /locus_tag="GVO03_RS04360"
                     /old_locus_tag="ScFU1_08440"
     CDS             1460..2248
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044313.1"
                     /locus_tag="GVO03_RS04360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08440"
                     /product="TrmB family transcriptional regulator"
                     /protein_id="WP_003044313.1"
                     /transl_table=11
                     /translation="MESLLQAMKYFGFTEMETKVYVSLLEFGPSTGYEISKKSGVPRSK
                     VYNILEKLVKEGIIFDNHADIKLYTALSVEEMLLRIDNKTEKQFQQIEHYLSRITEKQK
                     SYELWKVDHLQSVIEKVHFLINHANESLLIQIWEPDLTEEIVQLLQKAEERLNVFILIL
                     FSDNHHYDLPLKHYYKHGFEQAKRHDMGGRWINIIADDDSVLFGTLEDNINIIWTHHPA
                     MLSLAKEYIKHDAYTLKIIEDHREMLEKTYGPDLGQIREI"
     gene            2277..3134
                     /locus_tag="GVO03_RS04365"
                     /old_locus_tag="ScFU1_08450"
     CDS             2277..3134
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044310.1"
                     /locus_tag="GVO03_RS04365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08450"
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="WP_003044310.1"
                     /transl_table=11
                     /translation="MSIKNILLILVIAVNAYFAYILVKDLLSHKKETMAEAAPTAVMPF
                     SSAIIFFLSTIGISDFAISTSLYPKLNWTSVKKLPGTLNAQCTIPVAVMALAYIQSIKV
                     GVLTLAVCIICQVIGSYFGAKFAIKLPAEKIKYYIGVGMIIAAIIIVGGLLGMLPKGGN
                     ATELTGLKLVVAGILLFIFGALNNIGIGSYALTMVTVYLLGMSPAVSFPIMMGAATFSV
                     PVGSVQFVKSGEYSRKITLFTSTFGVLGVLAAVHFVTSLNIDALKWVVAAILIYSSYGM
                     LKKV"
     gene            3152..4147
                     /locus_tag="GVO03_RS04370"
                     /old_locus_tag="ScFU1_08460"
     CDS             3152..4147
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044307.1"
                     /locus_tag="GVO03_RS04370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08460"
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="WP_159304851.1"
                     /transl_table=11
                     /translation="MLVLTKEDIKACFSMSDAIEASKASVKAYSAGQAKVPLRVNLPVN
                     QFNGQSLYMPAAVEGDINAVGVKVVSVYPDNIDRGLPSVPASMVVVDAETGVVSAIMDG
                     TFLTQLRTGAVQGAATDLLARKDAKIAAIIGTGGQAIQQSLAMLTVRSIEELRVYDINL
                     ERAQAFAKELDDEFGKLFNTKFIAVSSPKEAVSGADIITSVTTSKRPTFDAQDIKPGTH
                     VNGVGAYTSEMIELPAEIFAKAETIVFDTVDGVVNEAGDVMNALNTKAIQETDITGELG
                     QLVLGSITGRQSKQGITLFKTVGSAVLDVVTAQLIVKKALEKGIGTIIDL"
     misc_feature    4673..4688
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.0"
                     /tool="antismash"
     gene            4706..6400
                     /locus_tag="GVO03_RS04375"
                     /old_locus_tag="ScFU1_08470"
     CDS             4706..6400
                     /EC_number="5.4.2.-"
                     /GO_function="GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /locus_tag="GVO03_RS04375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08470"
                     /product="phospho-sugar mutase"
                     /protein_id="WP_159304852.1"
                     /transl_table=11
                     /translation="MTYKEVYQEWLQNDALSEDIKADLVAIKDNETEIQDRFYKTLEFG
                     TAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDYGPEAVARGIAVSYDVRYQSQTFAEL
                     TCSIMAANGIKAYIYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQEGS
                     QILDDIADQISGHMAAITCYEDIKQIPFEEGLASGLISYIDDSIEESYKQEVLGLTIND
                     TAIDKSVRVVYTPLNGVGNLPVREVLMRRGFDNVYVVPEQELPDPDFTTVGYPNPEVPK
                     AFAYSEKLGREVDADILIATDPDCDRVALEVKNPAGKYVFLNGNKIGALLSYYIFSQRA
                     ALGNLPERPVLVKSIVTGDLSRAIASYYGIETVETLTGFKNICGKANEYEVTKAKTYLF
                     GYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKERGQTLLDVLETIYTTFGYYNERQI
                     ALELEGVEGQERIARIMDDFRQNSLEAVAEMALKKTIDFIDGYQEFPKQNCLKYYFDEG
                     SWYALRPSGTEPKIKLYLYTIGQTQADSLAKLDAIEAACRLKMEQVD"
     gene            6619..7473
                     /locus_tag="GVO03_RS04380"
                     /old_locus_tag="ScFU1_08480"
     CDS             6619..7473
                     /GO_function="GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /locus_tag="GVO03_RS04380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08480"
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="WP_003044302.1"
                     /transl_table=11
                     /translation="MTEIIDGKALAQKMQSELTAKVKQLKQEKTIVPGLVVILVGDNPA
                     SQVYVRNKERAALAAGFKSETVRLSESICQEELIAVIERYNEDETIHGILVQLPLPNHI
                     NDKKIILAIDPKKDVDGFHPMNTGHLWSGRSLMVPCTPAGIMEILREYEVDLEGKHAVI
                     IGRSNIVGKPMAQLLLDKNATVTLTHSKTRHLEDVCRHADVLIVAIGQGHFVTKNFVKE
                     GAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTITMLLEQTYQAALRS
                     VSR"
     gene            7470..8318
                     /locus_tag="GVO03_RS04385"
                     /old_locus_tag="ScFU1_08490"
     CDS             7470..8318
                     /GO_function="GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044300.1"
                     /locus_tag="GVO03_RS04385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08490"
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="WP_159304853.1"
                     /transl_table=11
                     /translation="MISDYKLARKVILPRQKETHKGSYGRLLLIGGLAPYGGAIIMAAL
                     AALKSGAGLITVATDQTNISALHSHLPEAMAFSVDDKQLLLAQLEAADLVLIGPGLSEN
                     STAHSLFELVVSHIKGHQLLLVDGSALTIAAQKEYSFQHLAAPVILPPHQKEWERLSGL
                     TIERQTVTNNQKALQQFPQGTILVAKSSATKLYQDELIYELIVGGPYQATGGMGDTLAG
                     MIAAMAVQFKQVDLFDRVACATYLHSAIADQLAQEAYVVLPTAISEEIPKWMKAISEAD
                     E"
     gene            8468..9808
                     /gene="xseA"
                     /locus_tag="GVO03_RS04390"
                     /old_locus_tag="ScFU1_08500"
     CDS             8468..9808
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /locus_tag="GVO03_RS04390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08500"
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_159304854.1"
                     /transl_table=11
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQYF
                     SLKDEGAVIQATMWAGIYKQLGFELEEGMKVNVIGRVQLYEPGGSYSIVIEKAEPDGIG
                     ALALQFEQLKKKLTAEGYFEQKHKQSLPQFVSKIGVITSPSGAVIRDIITTVSRRFPGV
                     EILLFPTKVQGDGAAQEVVANIGRANEREDLDLLIVGRGGGSIEDLWAFNEEVVVQAIF
                     ESKLPVISSVGHETDTTLADFVADRRAATPTAAAELATPVTKTDLVSWIAERQNRSYQA
                     CLWRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSLTLASTLKDRLRSAKESKVQ
                     LDHALAGLQLPAKIERYQDRVDTASRLLIANMTSQYDSQLARFEKAQDTLLSLDTSRII
                     ARGYAMIEKNHELVASVEQIRQGDQLTINMRDGQLDVEVKDVKNENI"
     gene            9786..10001
                     /locus_tag="GVO03_RS04395"
                     /old_locus_tag="ScFU1_08510"
     CDS             9786..10001
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /locus_tag="GVO03_RS04395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08510"
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_003044293.1"
                     /transl_table=11
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKEL
                     QKTLQEAEKTLVKVMQTDGTEVDMDD"
     gene            10001..10873
                     /locus_tag="GVO03_RS04400"
                     /old_locus_tag="ScFU1_08520"
     CDS             10001..10873
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /locus_tag="GVO03_RS04400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08520"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_159304855.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 2e-51, bitscore:
                     168.7, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKLARIDEAIRRYYKSGVQSVSEELIEAILYSVDSGGKRIRPLL
                     LLEVIEGFGLSLVDAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTSHKQFGETT
                     AILAGDSLFLDPFGLIAKADLKNDIKLALIQELSLASGTFGMVGGQMLDMKGENQVLTV
                     AQLSAIHTNKTGKLLAFPFIAAGLVAEQAESICQQLGQAGRLVGLAFQVRDDILDVTAS
                     FEDLGKTPQKDLVADKATYPNLLGLEQSYRILNDSLDQALLIFQELETVVGFKAQTIMK
                     LIEGLRLNA"
     gene            10866..11693
                     /locus_tag="GVO03_RS04405"
                     /old_locus_tag="ScFU1_08530"
     CDS             10866..11693
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /locus_tag="GVO03_RS04405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08530"
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="WP_003044288.1"
                     /transl_table=11
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPGE
                     KIDKSTELKLKGEKLKYVSRGGLKLEKGLRVFDLSVADQIGIDIGASTGGFTDVMLQDG
                     AKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFSEGQPVFASIDVSFISLRL
                     ILPALHNVLSDQGHVVALIKPQFEAGREQIGKKGIVKDKAIHEKVIQKVVDFALDYGFT
                     VKGLDFSPIQGGHGNIEFLAYLVKSQRPEGLAPQTIQEVITKAHKEFEKHEKE"
     gene            11680..12150
                     /locus_tag="GVO03_RS04410"
                     /old_locus_tag="ScFU1_08540"
     CDS             11680..12150
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /locus_tag="GVO03_RS04410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08540"
                     /product="arginine repressor"
                     /protein_id="WP_159304856.1"
                     /transl_table=11
                     /translation="MKKNERLELIKKMVLTHSIETQHDLLALLAEHGLELTQATISRDM
                     NEIGIVKIPSGSGRYVYGLSQDSGKKVIQGPESIKSTILEMSDKIEGLEQHLYLKVVPG
                     NSKLIKRYLLADFAEAIFSLIADDDSLLLIAKSAADADKIRQEVSRWMQGLT"
     gene            12169..13830
                     /gene="recN"
                     /locus_tag="GVO03_RS04415"
                     /old_locus_tag="ScFU1_08550"
     CDS             12169..13830
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /locus_tag="GVO03_RS04415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08550"
                     /product="DNA repair protein RecN"
                     /protein_id="WP_159304857.1"
                     /transl_table=11
                     /translation="MLLEISIKNFAIIEEISLNFENGMTVLTGETGAGKSIIIDAMNMM
                     LGARASTEVIRHGANKAEIEGFFSVDANPALAACLEASGIAMEEDLIIRRDIFANGRSV
                     SRINGQMVNLATLKQIGQFLVDIHGQHDQEELMRPQLHQHILDTFGDKAFGQLKEAYQL
                     IFDRYKNLRKQVLDKQRNEKEHKERLDMLAFQMAEIEAAALSRGEDERLYQERDRLLNH
                     KKIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFAPDYKAMSTTISEAYYILEEV
                     SKQLSDTIDQLDFDGDRLQEIEFRLDTLNSLTRKYGGDVTDVLDYYDNIVKEYQLLTGD
                     DRSSGDLESELKVLEKQLVAAATDLSEYRHQLANRLEADIKAELRELYMEKADFKVQFT
                     KSKFNREGNENLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISKKEDKTSIVFD
                     EVDTGVSGRVAQAIAQKIYKIGCHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKV
                     RLLTSEERVEEIAKMIAGADLTEAALTQARELLVKH"
     gene            14028..14960
                     /locus_tag="GVO03_RS04420"
                     /old_locus_tag="ScFU1_08560"
     CDS             14028..14960
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012948.5"
                     /locus_tag="GVO03_RS04420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08560"
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_159304858.1"
                     /transl_table=11
                     /translation="MSNKKVYLSSIIVLATLVCGPNTVTAEQFSTGKVSSYQASLVEGE
                     LTAQEALKQRQPQAGESSKASFEDFPEDSQTQEAPLDSKVEFEPIPEIAEVEPISLDSG
                     QVRSSESEKSELKALPIVPRNEEINWAAAANARLPISEEDSLEIDNPKPHVRSLPSLTF
                     QYMVAKHNLNQKMDALNQATKKLALLMTQKPDLTTQVYTIGHTFGEVLATHYEKKTVYK
                     SKPSNQTVGTPSAIIDELRKLISHIGDSKQEIKVPYNRKISMITVKKKVSLPATGGKVS
                     LSLTLLGLASLITAPAMALRHTNKLGNFD"
     gene            15202..16041
                     /locus_tag="GVO03_RS04425"
                     /old_locus_tag="ScFU1_08570"
     CDS             15202..16041
                     /GO_function="GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264715.1"
                     /locus_tag="GVO03_RS04425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08570"
                     /product="DegV family protein"
                     /protein_id="WP_093998796.1"
                     /transl_table=11
                     /translation="MGTIKIVTDSSITIEPELIEALDITVVPLSVMVDSKLYSDNDLKE
                     EGYFLSLMKASKSLPKTSQPPVGLFAETYENLVNKGATNIVAIHLSPALSGTIEASRQG
                     AEIAEAPVAVLDSGFTDQAMKFQVVEAAKLAQAGASLEEILAAVQAIKSKTELYIGVST
                     LENLVKGGRIGRVTGVLSSLLNVRVVMELKNDELKTLVKGRGNKTFIKWLDSYLAANND
                     RSIAEVAISYAGEADLAMALKEKIATQYDGSIPVLETGSIIQTHTGEGAFAVMVRYE"
     gene            16034..16876
                     /locus_tag="GVO03_RS04430"
                     /old_locus_tag="ScFU1_08580"
     CDS             16034..16876
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /locus_tag="GVO03_RS04430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08580"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_201281564.1"
                     /transl_table=11
                     /translation="MNNRRFLSGLLFFLVSLGFAFLLLNILIPKSDSRLKKSDFLKSEQ
                     VAINYVAIGDSLTEGVGDLTNQGGFVPLLSSDMGEYFRAKVSHQNYGVSGNTSQQILDR
                     MTKEKEIKTALKEADVMTLTVGGNDVMAVIRKHLADLQVSSFKKPARQYQERLRKILDL
                     ARKDNKDLPIFVLGIYNPFYLNFPELTDMQKVIDDWNDKTKEVLEEYDHVYFVPINDLL
                     YKGVNGQEGIVQSSGDQTTVVNDALFTGDHFHPNNTGYQIMSDAVMEKIRKHEKDFNP"
     gene            16854..17441
                     /locus_tag="GVO03_RS04435"
                     /old_locus_tag="ScFU1_08590"
     CDS             16854..17441
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /locus_tag="GVO03_RS04435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08590"
                     /product="YpmS family protein"
                     /protein_id="WP_159304860.1"
                     /transl_table=11
                     /translation="MKKTLTLNWWKWGFLLLLAFNTAFLLVIGSRLIQVREPESELIAQ
                     KATKDVKIGTFTTTREQLNETVASYLKDYQSEKMHYKFYATSSSILFEGTYQLLGYEVP
                     LYIYFQPHRLDSGAVQLQVISFSVGTLPLPEKDVLQYLKSSYHLPKFVKVMPQQSAIVV
                     NLQEIRNDAKVYLKAKKIDLFKDEISFNIYKK"
     gene            17540..17815
                     /locus_tag="GVO03_RS04440"
                     /old_locus_tag="ScFU1_08600"
     CDS             17540..17815
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1132: DNA-binding
                     protein HU 1"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /locus_tag="GVO03_RS04440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08600"
                     /product="HU family DNA-binding protein"
                     /protein_id="WP_003044261.1"
                     /transl_table=11
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSAIEAFLAEGEKVQL
                     IGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(<17882..18130)
                     /locus_tag="GVO03_RS10560"
                     /old_locus_tag="ScFU1_08610"
                     /pseudo=""
     CDS             complement(<17882..18130)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767272.1"
                     /locus_tag="GVO03_RS10560"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="ScFU1_08610"
                     /product="helix-turn-helix domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAKPLGETFRELR"
     misc_feature    complement(18249..18262)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.67"
                     /tool="antismash"
     misc_feature    18283..18296
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 26.23"
                     /tool="antismash"
     misc_feature    complement(18342..18356)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.54"
                     /tool="antismash"
     gene            18347..20209
                     /locus_tag="GVO03_RS04450"
                     /old_locus_tag="ScFU1_08620"
     CDS             18347..20209
                     /EC_number="7.2.2.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015662 - P-type ion transporter activity
                     [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /locus_tag="GVO03_RS04450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ScFU1_08620"
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_159304861.1"
                     /transl_table=11
                     /translation="MTIRAWMTDHLHLMETLACLVFIIIGLTFLHPFPQVASAIFIIAF
                     LIGGYESAKAGLLDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLEEM
                     AMEKSKDAISALMSLTPDTARRYQEDGRILEVETKTLSVGDRLQVRKGETVPIDGQLLS
                     PFGQFDESMVTGEPITVDKTKGQELIGGTINQGQTIDMLVTVENDNTLFAKIVSLVESA
                     QGQKSKTATFIERLEDSYVKFILVLVPTFIFFSHLVLAWTWLDAFYRGMILLTVASPCA
                     LIASSTPASLSAISRAARKGLIIKGGDIIDNMGDIKAVVMDKTGTLTQGKPSVVNAHYL
                     EDELLVNRLVKGAEAASTHPISKALLDYTEKLEPLTFDQLEEISGKGFQGFYQGQEWRI
                     GKKSFILEKVQDLSAFEKTIQEEENQGKTLIFVSCDHQLVAYYVLLDDIKIESQRAIET
                     LHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTKYGFVAMVGD
                     GINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKMKTIVKQNIVL
                     ALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
ORIGIN
        1 gaccgagtca tcttcatgga tggtggggtt attgtagaag aaggcaaccc tagccaactc
       61 tttgaccaaa ccaaagaaga ccggaccaaa gattttttga gcaaggtttt ataagcggct
      121 tgatctagct tgagcatcca tttgctcagg ttttttgatt gcaacaagac tgaacgactg
      181 ttagcaagaa agcttctgtg tcagattgcc atttcttttt agagttattc ttatatgaga
      241 ttaaaaaggc aaaaaactta aagcgagata cgttagagac aagtgaatgt gttataatta
      301 acactgtcgt taaaaggagt acgtatgatc aaattatctc atgcaaaagt tggaatagct
      361 tatcaggttg agggcattga tttgcctcag ggccaccaga agcatctggc tcaccttggc
      421 attactaaag gaagtcaggt gaaagtgatg tcacaaacca aacagagtgc gattgtgatg
      481 gtcaaaacca gtcgtctagc ttttgatgat agtattctag aaactattat ggttagtgag
      541 gtgatagggg aggagagcaa agcctgccct tgtctgaact agctgtttta gatgtggcta
      601 ttattgatgg catttatgct agtaaagaaa ccaagcgtcg tttaatggat atggggttaa
      661 tcagaggaac tcgttttcag ttgcgtaagc gggcacccct aggccgatcc cttagaaatt
      721 agtctgaggg gttatggatt aagcttgcgc cagtctgaag cccaactaat ctctggacga
      781 aagctagaag atgaggatac actatgacaa aaatagcctt gattggcaat cccaatagtg
      841 gaaaatccag cctgtttaat ttattgacga gaaccaacca acgggtggga aattggcccg
      901 aagtttagta ttgttgggac agccagttac ctgatggatt gttttggctg gagggtgtgt
      961 attagcactg gtttatgtta ttgtggaaag gtcaacccta gaccctagtc aagtgattac
     1021 cagatctcat cttgaacgta ttcgtcagga ggaaaaagtt aatgtcaacg cttattcttg
     1081 caacctttat aggagtttta atcattctaa gccttacccg ttacagtaac aaaagggatc
     1141 tcgtggggat tgcaactgtt cttgtcctgt gagggaagat atgaaaaaga cctatatgaa
     1201 taaaacatag ttccttagaa aggttatgga gattgggatc attattttgc ttagaggctg
     1261 ttcttgtgaa cagcctcttt ttgtatgtca aagagtagtt acttgtcaga taaaggttta
     1321 ggggatagag catctttgat aaaaaaagaa tctcacttac tctgaaaatt tgtgatcttt
     1381 ttaacttttt attgcaatcg ttttcataat gtattataat agttaccaag gtagctacta
     1441 aatctaataa ggagattcta tggagtcatt gttgcaagcg atgaagtatt ttggatttac
     1501 tgagatggaa accaaggttt atgtttcgct tttagaattt ggaccctcga ccggatatga
     1561 aattagtaag aaatcgggtg ttcccagatc aaaagtctat aatattttgg aaaaattagt
     1621 gaaagaggga attatttttg acaaccacgc tgatataaag ctctacactg ctctgtcagt
     1681 ggaagaaatg ttattacgca ttgacaataa aacggagaaa caatttcaac agatagaaca
     1741 ttatttaagc cgtattacag aaaagcaaaa gagttatgaa ctctggaaag tagatcattt
     1801 gcaaagtgtc attgaaaaag ttcatttttt aattaatcac gctaatgaaa gtttacttat
     1861 ccaaatttgg gagcctgatt tgacagaaga gatagttcaa ctgttgcaaa aggctgagga
     1921 gaggttaaac gtttttattt taattttatt cagcgataat caccattacg atttacccct
     1981 gaaacattac tataagcatg gttttgaaca agctaaaaga catgatatgg gtggtcgttg
     2041 gattaatatt attgcagatg atgattcggt tctttttgga accctggagg ataacatcaa
     2101 tattatttgg acccatcatc cagccatgct atcactggct aaagaataca ttaagcatga
     2161 tgcttatacc ttgaaaatta ttgaagatca tcgagagatg cttgaaaaaa cttatggccc
     2221 tgatttaggt caaatacgag aaatctaatt attgaaaatg aaggagtact actattatgt
     2281 ccattaaaaa tatcttattg attcttgtta ttgcagtgaa tgcctacttt gcctatatcc
     2341 tagtcaagga tttattatcg cacaagaaag aaacaatggc tgaagctgca ccaacagcag
     2401 ttatgccttt ttcaagtgct atcatctttt tcctatcaac gattggtatc tcagactttg
     2461 ccatcagtac aagtttatac ccaaaattaa actggactag tgttaaaaaa ctgccaggaa
     2521 ccttgaatgc tcagtgtacc ataccggtcg ctgtgatggc cttggcttat attcagagta
     2581 tcaaagtagg cgtcttaacc ttagcggtct gcattatctg tcaagtgatc ggatcttatt
     2641 ttggtgcaaa atttgccatt aaattgccag cagaaaaaat taaatattac atcggagttg
     2701 ggatgattat tgctgcaata atcattgttg gtggtctatt aggaatgtta ccaaaaggtg
     2761 gtaatgcaac agaattaaca ggtttgaaat tagtcgttgc tggtattctc ttgtttattt
     2821 ttggagcatt aaataatatc ggtattggtt catacgcttt aacgatggta actgtttatc
     2881 ttcttggaat gagtccagca gtttccttcc caattatgat gggagccgca accttctctg
     2941 ttccagttgg tagcgtacaa tttgttaaat ctggagaata cagtcgaaaa attactcttt
     3001 tcacctctac ctttggggtg ttaggtgttt tagccgccgt tcactttgta acgtcattaa
     3061 atattgatgc cttgaagtgg gtagtagcag ctattcttat ttatagttcg tatggtatgt
     3121 taaaaaaagt ttaagttaaa ggagcatgac catgttagta ttaacaaaag aagatattaa
     3181 agcctgtttt tcaatgtcag acgctattga ggcaagcaag gcctcagtca aagcatattc
     3241 tgctgggcaa gcaaaagttc ccttacgtgt gaatttacca gttaatcagt ttaatggcca
     3301 aagtctttat atgcctgcag ctgttgaagg tgatattaat gctgttgggg ttaaggttgt
     3361 ttcagtttat ccagacaata ttgatagagg tttgccatcg gtacctgctt cgatggttgt
     3421 ggtagatgct gagactggtg tggtatcagc aatcatggat ggaacttttt taactcaact
     3481 aagaactgga gcagtacagg gggcagctac tgatttgtta gcgcgaaaag atgctaagat
     3541 tgctgcaatt attggtacag gaggtcaagc cattcaacaa tcattagcga tgttaaccgt
     3601 ccgatctatt gaagaattgc gtgtttatga cattaattta gagcgtgctc aagctttcgc
     3661 taaagaactt gatgacgaat ttggtaaact attcaacact aaatttatag cagtgtcgtc
     3721 tccaaaagaa gcggtttcag gtgctgatat tatcacgtca gtgacaacgt caaaaagacc
     3781 gacattcgac gcacaagata ttaaaccagg tactcatgtt aatggtgtgg gagcatatac
     3841 ttcagaaatg attgaacttc ctgcagaaat ctttgccaag gcggaaacca ttgtttttga
     3901 tactgttgac ggtgtggtta atgaggcagg cgatgtcatg aatgctctga acacaaaggc
     3961 tattcaagaa acagatatta caggtgaact tggacagcta gtgctcggaa gtattactgg
     4021 tcggcaaagc aaacaaggta taacactctt taaaacggta ggatctgctg tcttagatgt
     4081 tgttacagct caactgattg ttaaaaaggc tcttgaaaaa ggaattggaa caatcattga
     4141 tttgtaatac cttatgttga attcaggata ggaataaatg gatgtgatct tttgacgata
     4201 aggctgaatt ttacctctat gagaggtcaa tcagattgtt caaaatcact tgccctttgc
     4261 tttataggga cttattgatt cgcctaactg ttagttgatt aaattgagct tgttaatggt
     4321 gtgacactag cattattagc aagctctttt tatttagtca cttttatcag taagcgatga
     4381 gattagattg agacgcttta gggtataaaa gcaagaaaat ctaatttacc ttattcataa
     4441 agtctcccct tacctatggc ctgttattaa ccatgctgcg caaggcttta ggtttatcgg
     4501 aaagtaggaa taagcaatcg aaaaagtgcg aacggaagag ggaacgtcct aggttgcagg
     4561 aaatcaatat taatataggt taatttataa aataagttga ctttataaat gttatttgtt
     4621 actattcagt tagctccttc aggggaaaaa atctcttgtc aggagagtga tttttaccat
     4681 ttggtcttag aagaggatga gtaatatgac ttacaaagag gtttatcaag aatggctgca
     4741 aaatgatgcg cttagtgaag acattaaggc agatttagtg gcaatcaagg ataatgagac
     4801 tgagattcaa gatcgttttt acaagacact tgaatttgga acagcagggc taagaggcaa
     4861 actcggtgca ggaaccaatc gaatgaatac ttacatggtt ggtaaagctg cgcaagcttt
     4921 agccaatact atcattgatt atggtcctga ggcagttgca agaggcattg cagttagcta
     4981 tgatgtgcgc taccaatccc aaacatttgc agagttaacg tgctctatca tggcggcgaa
     5041 tggtatcaag gcttatatct ataagggcat tagaccgaca ccgatgtgtt cttatgctat
     5101 tcgtgcctta ggttgtatct caggtgtcat gattaccgct agtcataatc ctcaagctta
     5161 taacggttac aaggcttatt ggcaagaagg ctctcaaatt ctagatgata ttgcagacca
     5221 gatttcaggc cacatggctg ccatcacttg ctacgaagac attaagcaga taccttttga
     5281 ggaaggctta gctagcggtc ttatcagcta tattgatgac agtatcgaag agtcctacaa
     5341 gcaagaagtg cttggtttaa ccattaacga cacagctatt gataagtctg ttcgtgtggt
     5401 ctatacgcct cttaatggcg ttgggaattt accagtgcgt gaggtcttga tgcgtcgtgg
     5461 ttttgacaat gtttatgtgg tccctgagca agaattacca gaccctgatt ttacaacagt
     5521 tggctaccct aatcctgaag ttcctaaagc ttttgcctac tcggaaaaat taggtaggga
     5581 agttgatgcg gacattttga ttgcaactga ccctgactgt gaccgtgtag ccttagaagt
     5641 taaaaatcca gctgggaaat atgtcttttt aaatggtaat aaaattggag cactcttgtc
     5701 ttattacatt ttttcacagc gtgcagcctt aggcaatttg ccagagcgtc ctgttttggt
     5761 caagtctatt gtgacaggtg atttgtcacg ggctattgct agctattatg gtattgaaac
     5821 tgttgaaacc ttgactggtt ttaaaaatat ttgtggcaag gcaaatgaat atgaggtgac
     5881 caaagccaaa acctacctct ttggttatga agaaagtatt ggtttttgtt atgggacttt
     5941 tgttcgggat aaagatgctg tcagtgcgtc aatgatgatt gttgaaatgg ctgcctatta
     6001 taaagaaaga ggtcaaacct tactggatgt tttagaaacc atctacacta cttttggtta
     6061 ttataatgaa cgtcaaattg ccttagaatt agaaggtgtc gaaggacaag aacgtattgc
     6121 cagaatcatg gacgattttc gtcaaaactc tctagaagca gtggcagaga tggctttgaa
     6181 aaaaaccatt gatttcatag atggttatca agagtttcca aaacaaaact gcctcaaata
     6241 ttattttgac gagggttctt ggtatgcact tagaccctct ggcacagaac ccaaaatcaa
     6301 actttacctt tataccattg gccaaacaca agctgatagc cttgctaaat tagatgctat
     6361 tgaggcagct tgccgattaa aaatggaaca ggttgactag caactaaact aagaaagagg
     6421 caaaaaagtt actaagagca ctgaggttct tagctaaaat gattcaccta gggctgcaga
     6481 aacctttctg acagcctctt ttttggtttc ctgaaaaagg catactaaga gtaggcaacc
     6541 agtaacagtt tgcaaataga agagcagata atcaaatttt tgatataata gagagatgaa
     6601 aatgaagggg gaatgttgat gaccgagata atcgatggga aggccctagc ccaaaaaatg
     6661 caaagcgaac taacggccaa ggtaaaacaa ttaaagcaag aaaagactat tgtaccgggt
     6721 ctggttgtga ttcttgtggg ggataatcct gccagtcaag tgtatgtgcg caataaagaa
     6781 cgcgcagccc ttgctgctgg tttcaaaagt gaaaccgtta ggttgtcaga atctatttgc
     6841 caagaagaat tgattgccgt cattgaacgt tataatgaag atgaaacaat tcatggtatc
     6901 ttggtccaat tgcccttgcc aaatcacatc aatgataaga aaattatcct tgctatcgat
     6961 cctaaaaaag atgtggatgg ttttcaccca atgaatacag gtcacctttg gtcgggtcgt
     7021 tccttgatgg ttccttgtac gccagcaggt atcatggaaa tattgcgcga atatgaggtg
     7081 gaccttgaag ggaagcatgc ggtgattatc ggaagatcta atattgtagg taaaccaatg
     7141 gctcagctct tgttggataa aaatgcaaca gtaaccttga cgcattctaa gacacggcat
     7201 ttagaagatg tttgccgtca tgcagatgtc ttgattgtgg ccattggaca aggacatttc
     7261 gtcaccaaga actttgttaa agaaggagct attgtcattg atgtcggcat gaaccgtgat
     7321 gacaatggca agttaattgg tgatgtggcc tttgatgagg tggcagaagt tgcggcgaaa
     7381 atcactcctg taccaggagg tgttggtccc atgacgatta ccatgctatt ggaacaaacc
     7441 tatcaagccg cgctacggag tgttagtcga tgattagtga ttataaattg gctagaaaag
     7501 tgattttacc aaggcagaaa gagacccata aagggtccta tggacggcta ttgttgatag
     7561 gtggcttagc accttacggt ggtgctatca ttatggcagc tctagctgct ttaaaaagtg
     7621 gagcaggttt aatcactgtt gcgactgacc agaccaatat ttcagccttg cacagccatt
     7681 taccagaagc gatggctttt tcagtagatg ataagcaact cttgctggct caacttgaag
     7741 cagctgatct tgttttaatc ggtccaggac tttctgaaaa tagcacagca cacagcttat
     7801 ttgaattggt cgtgtctcac atcaaaggtc atcaactttt gttagttgat ggctctgctt
     7861 taacaattgc tgcccaaaaa gagtattcct ttcaacattt ggctgcgcca gtgatattgc
     7921 ccccccacca gaaggagtgg gaacgtcttt cgggcttgac aattgagcga cagaccgtga
     7981 caaacaatca aaaagcactg cagcaatttc ctcaaggtac gattttggtg gctaaaagtt
     8041 cggcaacaaa actttatcaa gatgagctga tttatgagtt aattgttgga gggccttatc
     8101 aagcaactgg tggtatggga gatactttgg caggtatgat tgcggcaatg gctgttcagt
     8161 ttaaacaggt tgatttgttt gatagggtag cttgtgcaac ctatcttcac tcagccattg
     8221 ctgaccaatt agctcaagaa gcttatgtgg ttttaccaac agccattagt gaagaaattc
     8281 cgaaatggat gaaggctatc agtgaggctg atgaataggg ctgcaacaag gctaataggt
     8341 gtttaaaagg agagctcaga cggctccttt ttctggtttt ctactcacta tttattctta
     8401 aaaatggtat aattgcatta gtatgcaatg ggactgaaac agttctaaat aagtgaaagg
     8461 tggcttgatg gcagattatt taaccgtcac tcatttgacg aagtatttaa aattaaaatt
     8521 tgaccgtgat ccctatttgg aacgggttta tctgactggt caagtatcca attttcgaaa
     8581 acgaccaact catcagtatt tttctttaaa agatgaaggt gctgtgattc aggcaaccat
     8641 gtgggcaggt atttataaac agttaggctt tgagcttgaa gaaggcatga aggtcaatgt
     8701 tattggccgt gttcaacttt atgagccagg tggttcctat tctatcgtga ttgaaaaagc
     8761 agagccagat ggtattggtg ctttggcctt gcagtttgaa caattgaaga aaaaattaac
     8821 agctgaaggt tattttgagc aaaaacataa gcagtcctta ccgcagtttg tgtccaaaat
     8881 tggggtcatt acaagtccta gtggtgctgt gattagagat attatcacga cggtttctag
     8941 gcgtttccca ggggttgaga ttttattatt cccaactaaa gtgcaaggtg atggagcagc
     9001 ccaagaagta gtggctaata ttggcagagc taatgagcga gaagatctgg atttactcat
     9061 tgtcggtcgt ggaggtgggt cgatagaaga cctttgggct ttcaacgagg aagtggtggt
     9121 tcaggcaatt tttgaatcga aacttccagt gatttcaagt gtggggcacg aaacagatac
     9181 aaccttggcg gattttgtgg cagatcgcag agcagctact ccaacagcag cagcagaact
     9241 agcaacacct gttacgaaaa cagaccttgt ttcttggata gcagagcggc aaaatcgatc
     9301 ttatcaagct tgcttatggc gaattaagca aaggcaagaa tgggtggata aactgtcaca
     9361 atctgttatt tttaggcagc cagaacgttt gtatgatgcc tacctgcaaa aaattgatcg
     9421 ccttagcctg accttggcaa gtaccctcaa agaccgctta cgaagcgcca aagaaagcaa
     9481 ggttcagtta gatcatgctt tagcaggtct tcaattacca gcaaaaatcg agcggtatca
     9541 agaccgtgtt gataccgcca gtcgtttgct gattgccaat atgactagtc aatatgatag
     9601 tcagctggcc cgctttgaaa aggctcagga tacccttttg tcactagata ccagtcgtat
     9661 tattgcgcgt ggctatgcga tgattgaaaa aaatcacgag ctggttgcct ccgtagaaca
     9721 gattaggcaa ggagatcagt tgacaattaa catgcgtgat ggacaattag atgtagaggt
     9781 aaaagatgtc aaaaacgaaa acatttgaag aaaacttaca agatttagaa accattgtga
     9841 acaaacttga aaatggggat gtcccattgg aagaagccat ttcagaattt caaaaaggaa
     9901 tgcttttatc aaaagaactc caaaaaactt tacaggaagc tgaaaaaaca ttggttaagg
     9961 taatgcaaac tgatggcaca gaagtggaca tggatgactg atggaaaaat tagctagaat
    10021 tgatgaggcg attcgtcgtt actacaaatc aggcgttcag tctgtatctg aagagcttat
    10081 tgaagctatt ttatattctg ttgatagtgg tggcaagcgc attcgaccac ttttattact
    10141 agaagttata gaaggatttg gcttatcgtt agtcgatgct cattttgact tagcagctgc
    10201 ccttgaaatg attcacacgg gaagtcttat tcatgatgac ttaccagcta tggacaatga
    10261 tgattaccgt cgtggcaggc tgaccagcca caaacaattt ggcgaaacca ctgctatttt
    10321 ggcaggggat agtttgttct tggacccctt tggtctgatt gctaaagctg atttgaaaaa
    10381 tgacatcaag cttgccttga ttcaggagct ttcccttgct tcaggcactt ttggcatggt
    10441 tggtgggcaa atgctagaca tgaaagggga aaatcaggtg ttgaccgtgg ctcagttatc
    10501 tgctatccac actaataaaa caggaaaatt attagctttt ccctttatcg cagctggtct
    10561 tgtggcagaa caggcagaaa gtatctgtca acaattaggc caagcaggac gtctggtcgg
    10621 acttgctttt caggttagag atgatatttt agatgtaacg gctagttttg aagaccttgg
    10681 aaagacccct caaaaagatt tagtagctga caaggccact tatcctaact tgcttggttt
    10741 agagcaatct tatcgtattt taaacgatag tttagatcaa gcgctgctga tttttcaaga
    10801 actagaaaca gtagtaggct ttaaagctca aaccattatg aaactgatag aagggttgcg
    10861 actcaatgcc taaagaaaga gtagatgtat tagcctacaa gcaaggcttg tttgagacaa
    10921 gagaacaagc caaacgtggt gttatggcag gtttggtggt ctctgtgatt aatggtcaac
    10981 gctatgacaa accaggagaa aaaattgaca agagcactga attaaaactc aaaggcgaaa
    11041 aactcaaata cgtaagccga ggtggcttga aattggagaa aggcttgcgg gtttttgact
    11101 tgtcggttgc tgatcaaatt ggtattgata ttggagcttc aacaggtggt ttcacagatg
    11161 tcatgttgca agatggggct aaactggtct atgccgtaga tgttgggacc aatcagttgg
    11221 tttggaaact cagacaagat ccgcgagtga gaagcatgga acaatataat ttccgttatg
    11281 ctcagccaga ggactttagc gaaggtcagc ccgtatttgc cagtatcgat gtgtcattta
    11341 tttccctccg cttgattttg ccagctctgc ataacgtttt gtcagatcag gggcatgttg
    11401 ttgccttaat taaaccgcaa tttgaggccg ggcgcgagca gattggcaaa aaagggattg
    11461 tcaaagacaa agctattcat gaaaaagtga tccaaaaagt agttgatttt gctctagatt
    11521 acggatttac agttaaggga cttgattttt ctcctattca aggtggtcat ggcaatatcg
    11581 agtttttagc ttatcttgtt aaatcgcaga ggccggaagg attagccccc caaactattc
    11641 aagaagtgat taccaaagcg cataaggaat ttgagaaaca tgaaaaagaa tgaacgactt
    11701 gaactgataa aaaaaatggt cttgacacat tctattgaaa ctcagcacga tttattggca
    11761 ttattggctg agcatggact agaattgaca caggccacca tttcaagaga tatgaatgaa
    11821 attgggattg tcaaaatccc ctctggaagt ggccgttatg tttatgggct ttctcaggat
    11881 agtggaaaaa aggttatcca aggtccagaa tccataaaaa gtactatttt agagatgtct
    11941 gacaaaatag aaggtcttga acaacatctt tatttgaagg tagttccggg caacagtaaa
    12001 ttgattaaac gttacttgct agctgatttt gccgaggcta tctttagttt gattgcagat
    12061 gatgacagct tactcttaat tgccaaatca gctgcagatg ccgacaagat tcgacaagaa
    12121 gtctcgcgtt ggatgcaagg attaacttag aaatacgggg agaaagtcat gttattagaa
    12181 atttccatta aaaattttgc tattattgag gaaatctccc tgaattttga aaatggtatg
    12241 acagttttaa cgggggaaac aggggctggg aaatccatca ttattgatgc catgaatatg
    12301 atgttgggag cacgtgccag cacagaggtt attcgtcatg gcgctaataa agcagaaatt
    12361 gaaggctttt tttccgtaga tgccaatcca gctcttgctg cttgtttgga agcatctgga
    12421 attgctatgg aagaggattt gattattcgc cgcgatattt ttgccaatgg tagaagcgtc
    12481 agccgtatta atggtcaaat ggttaatctg gcaactttga agcagattgg tcaatttttg
    12541 gtggatattc acggtcaaca tgaccaagaa gaattaatgc gaccacaatt acaccagcat
    12601 atcttggata cctttggtga caaagctttt ggacaattga aagaggctta ccagcttatt
    12661 tttgaccgct acaagaactt gcgcaagcag gttcttgaca agcaaagaaa tgaaaaggaa
    12721 cacaaagagc gtcttgatat gttggctttt cagatggcag aaattgaggc tgctgccttg
    12781 agtcgaggtg aagacgagcg gttatatcaa gagcgtgacc gcttattgaa ccataaaaaa
    12841 attgccgata ccttgaccaa cgcttatgtc atgttagata atgacgattt ttcaagccta
    12901 tccaatattc gttctagcat gaatgacttg ctgtctattg agcagtttgc ccctgactat
    12961 aaagccatgt caaccacaat ttcggaagct tattacattt tggaagaagt cagtaagcag
    13021 ttgtctgata ccattgatca actggacttt gatggtgatc gcttgcaaga aatcgaattc
    13081 cgcttggata cccttaatag tttgacccgt aaatatggcg gggatgttac tgatgttctg
    13141 gactattatg acaatattgt caaagaatat caactattaa caggagatga ccgttcctca
    13201 ggggatctag aatccgaatt aaaggttttg gaaaaacaat tggtagctgc agctactgat
    13261 ttgagtgagt atcgccacca actggcaaat cgattagaag ctgatattaa agccgaactt
    13321 agggaacttt acatggaaaa agctgatttt aaagtgcagt ttactaaatc aaagtttaac
    13381 cgtgaaggta atgaaaacct agagttctat atttcaacta acccaggaga aggtttcaag
    13441 ccactggtta aagtcgcttc tgggggtgaa ctatctcgtt tgatgttggc catcaaggct
    13501 gctatctcta aaaaagaaga taaaaccagt attgtttttg acgaggtgga tacgggtgtt
    13561 tctggccgtg tcgctcaagc gattgcccag aaaatttata aaattggctg tcatgggcaa
    13621 gtccttgcta tttctcattt gccacaggtc attgccatag cggattatca gtattttatt
    13681 tctaaagaaa gtaaagagga atcaaccgtt tctaaggtca gattattgac gtcagaagaa
    13741 cgtgttgagg aaattgctaa aatgattgct ggtgcagatc tgacagaagc agcacttact
    13801 caggcgcgtg aactgcttgt caaacattaa aaagaggata agttccattt agttgatgac
    13861 accttttaaa agaaaagttg ctgagcttac ttgtttgtag ttctaggaat aacacttcct
    13921 aaagattatc taacattcac tttggcggct tctttttagt gaacactctt gacaaacccc
    13981 tgataaatag tagaataaat aagataattt caatctagga gccatcaatg tctaataaaa
    14041 aagtatattt atcaagtatt attgtattag caaccttggt gtgtggtcct aacactgtta
    14101 cggctgaaca gttttcaaca ggtaaggtta gtagttatca agcaagtctt gtcgaaggtg
    14161 agttaacagc tcaagaagca ctgaaacaac ggcagcccca agcaggtgaa tcttctaaag
    14221 ccagttttga ggattttcca gaagactctc aaacccaaga agctcctctt gattccaaag
    14281 ttgaattcga gcctattcct gagatagcag aggtcgagcc gatttctctt gattctggtc
    14341 aggtcaggtc ttctgagtca gagaagtcag aactgaaggc gctgccaata gtcccccgga
    14401 atgaggagat taactgggct gctgctgcga acgcacgctt acctatctct gaagaggatt
    14461 ccttggagat tgacaatcca aaaccacatg tcagaagctt gccaagtctg acctttcagt
    14521 atatggtagc caaacacaat ttgaatcaaa aaatggatgc tctcaaccaa gcaacgaaaa
    14581 agttagccct cttaatgaca caaaagcctg acttgactac tcaggtttat acgattggtc
    14641 atacttttgg agaagtgttg gcaactcact acgaaaagaa aacagtctat aaatcaaagc
    14701 catctaatca gaccgttggt acgccaagtg caattataga tgaattgaga aaactgattt
    14761 ctcacattgg tgattctaaa caagagatta aggtgcctta taatcgaaaa attagcatga
    14821 ttaccgttaa gaaaaaggtc agcctaccag caacgggtgg caaagtttct ctttcactta
    14881 ccttacttgg tttagcctcc ttaataacag cacctgctat ggcattaaga catactaaca
    14941 agttggggaa ctttgattaa tcaagccact tatgttcatt agtaaagatg atcagacaga
    15001 aagtcaactt attaaaggtt ggtcttctgt cttttttatc tttcttcata tggataaatt
    15061 tcccattcat cggtaagtga ttgctgtact tgaaaaaaac gtttttccaa gctttatatc
    15121 ataagtattc ctaattttga tttatttgct aaggagtgat aaaataagat gatgagaaac
    15181 tagtctagaa tggaaaaata catgggaact attaaaattg ttacagattc atcaataacc
    15241 attgaaccag aactaattga ggcactcgat attacagtag tgcccctatc tgttatggtt
    15301 gatagtaaat tatattctga taatgactta aaagaagaag gctatttttt aagccttatg
    15361 aaggctagca aatcccttcc taaaaccagt cagccaccag ttggtctttt tgctgaaact
    15421 tacgaaaatt tggtcaacaa aggcgcaact aacattgttg ctattcatct ctcgccagcc
    15481 ctctcaggta ctattgaagc ttcacgacaa ggagccgaaa ttgctgaagc accagtcgcg
    15541 gttttggatt ctggctttac agaccaagcc atgaaatttc aggtggttga ggcagcaaaa
    15601 ttagcacaag caggggccag tcttgaagag atcttggcgg ctgtccaagc cattaagtca
    15661 aagacagagc tctatatcgg ggtttcaact ttagaaaatc tagtaaaggg tggacgaatt
    15721 ggtcgtgtta ctggtgtcct tagttcctta ctcaatgtta gggtggttat ggaactgaaa
    15781 aatgatgagt tgaaaacact tgtcaaaggt cgggggaaca aaacctttat caaatggcta
    15841 gacagttatt tggcagctaa caatgatcgt tcaattgcag aagtggctat ttcttatgcg
    15901 ggagaagctg acttggctat ggctctaaaa gaaaaaattg caacccaata tgatgggtct
    15961 atccctgttt tagaaacagg ctctattatc caaacccaca cgggtgaggg agcttttgcg
    16021 gtcatggttc gctatgaata atcgtcgatt tcttagcggt cttctattct tccttgttag
    16081 tctaggtttt gcctttttgc ttctaaatat cctgattcct aagtcagatt cacgcttgaa
    16141 gaaaagtgat tttttaaaaa gcgagcaagt ggcgattaac tatgttgcta tcggtgattc
    16201 actcactgag ggggtgggag atttgaccaa ccaaggtggt tttgtacctt tattgtcaag
    16261 tgacatggga gagtatttta gagccaaggt cagccaccaa aattatgggg tgtctggcaa
    16321 caccagccaa caaattctgg ataggatgac caaggaaaag gagatcaaga cagctttaaa
    16381 agaggctgac gtaatgaccc taaccgttgg aggcaatgat gtcatggcgg tgattagaaa
    16441 acatctagct gatctgcaag tttccagttt taaaaagcca gctcgtcaat atcaagagcg
    16501 cttacgaaag atactggatt tggctagaaa agataataaa gaccttccta tttttgtttt
    16561 agggatttac aatcccttct acctgaattt tccagaatta accgacatgc aaaaggttat
    16621 tgatgattgg aacgacaaaa ccaaagaagt cctggaagag tatgatcatg tttattttgt
    16681 tcctattaat gaccttttgt ataaaggtgt caatggacaa gaaggtattg ttcaatcctc
    16741 aggggatcaa accacagttg ttaacgatgc cttgtttact ggggaccatt ttcaccccaa
    16801 taataccggc tatcaaatca tgtcagatgc agtaatggag aaaatcagaa agcatgaaaa
    16861 agactttaac ccttaattgg tggaaatggg gctttttgct cttactagct tttaataccg
    16921 ccttcttact ggtgattgga agtcgcctta tccaagtaag agaacctgaa tcagaattaa
    16981 tcgctcaaaa ggcgactaag gatgttaaaa ttggcacatt taccaccaca agagaacaac
    17041 ttaatgaaac cgtcgctagt tatcttaaag attatcaatc tgaaaagatg cactataagt
    17101 tttacgccac ctcttcttct attttatttg aaggcaccta tcagctttta ggctatgagg
    17161 tacctttata catttatttt caaccccatc ggctagatag tggagctgtc caattacagg
    17221 ttatctcctt ttcggtagga accttacctt tacccgaaaa agacgtgttg cagtatctga
    17281 aatcaagcta tcacttgcca aaatttgtga aggtgatgcc acagcaatca gcaatagtag
    17341 taaatttaca agagataaga aacgatgcta aggtctattt aaaagccaag aaaatcgatt
    17401 tatttaaaga tgaaatcagt ttcaatattt acaagaaata agctcaaata acgttaaata
    17461 cttgacctaa gcgctttttt tagatatgat aatatctgtt aaagcattac gcttactaat
    17521 tatttggagg atttgttaaa tggctaacaa acaagattta atcgcgaaag ttgcagaagc
    17581 aactgaactt acaaaaaaag attcagcagc agcagttgat gccgtatttt ctgcaatcga
    17641 agcttttctt gctgaaggtg aaaaagtaca attaatcggc tttggtaact tcgaagtgcg
    17701 cgaacgtgca gctcgtaaag gtcgtaaccc acaaactggt gcagaaattg aaatcgcagc
    17761 ttcaaaagtt ccagcattca aagctggtaa agctcttaaa gacgctgtta aataataaat
    17821 ttttaaaaag cccgtcatat caagctttct agcttgtgtg atgggctttt ttctgtgttt
    17881 tggggcattt ttgggacata aagcaaattt gttctgtttt tttgaagtca gaagccatat
    17941 ccataaattc tttcacttcg acatgcatcc tatctaaggc tactaaaaac ttattgaggg
    18001 aaatagctga ctgccctcgt tcaaagcgtg ataattggga caaggaaata gagtctcctg
    18061 caatttgttg taaggaaagg ttttgatttc atcttaactc acgaaaggtt tctcctaaag
    18121 gttttgccat ggttgataat cctcctttta tcagtatatc atttccttag ttagggataa
    18181 gtttttctca tatttgcaaa tagtagatgt agagattttt gaaaaaacaa gtctgcaaaa
    18241 aagtttagtt agaatcgttc taaattactt gacttattta tttagaatta ttataattaa
    18301 tatatcttaa agattgagat aacttttaat tgtgagggaa ctaaaaatga caatcagagc
    18361 atggatgaca gaccatcttc atttaatgga gactttagcc tgtctggtat tcataataat
    18421 cggactaacc tttttacacc catttccaca agttgcttcc gctattttca ttatagcctt
    18481 cttgattgga ggatatgaat cagccaaagc aggcttgctc gatttggtga agaacaaaca
    18541 cttgtcagtg gatattttga tgattttggc agctattggt gctggaatta ttggctattg
    18601 gctggagggt gctctgctta tttttatctt ttccttgtcc aatacgcttg aagaaatggc
    18661 catggaaaaa agtaaggatg ctatttctgc cttgatgtcc ttaacgccag atacagcacg
    18721 tcgttatcaa gaagatggtc gtattttaga agttgagacc aagaccttga gtgttggtga
    18781 tcgtttacaa gttcgtaagg gggaaactgt tccaattgac ggacagttac ttagtccttt
    18841 tggtcaattt gacgagtcta tggttactgg tgagccgatt actgttgata agacaaaagg
    18901 tcaagaactt attggaggaa ccattaacca agggcaaacg atcgatatgt tggttactgt
    18961 tgaaaatgac aatacccttt ttgctaagat tgtaagtttg gtagaatctg cccaaggtca
    19021 aaagagtaaa acagctacct ttattgaaag gttggaagat agttacgtca agtttatcct
    19081 cgttctcgtt ccaaccttta ttttctttag ccacttagtc cttgcttgga cttggttgga
    19141 tgctttttac cgaggcatga ttctcttaac ggtggcttcc ccatgtgccc taattgccag
    19201 ttctacacct gcaagtcttt cagctatttc tcgtgcagcc cgtaagggat taattatcaa
    19261 aggtggagat attattgata atatgggaga tattaaggct gttgtgatgg ataaaacggg
    19321 gacattgacc caaggaaagc cttctgtggt gaatgctcac tatttggaag atgagttgct
    19381 cgtgaataga ctggtaaaag gagcagaggc tgctagtacc caccctattt ctaaagccct
    19441 tcttgactac actgaaaaat tggagccact tacctttgac caattagaag aaatttctgg
    19501 gaaaggtttt cagggcttct atcaagggca agaatggcga attggtaaaa aatcctttat
    19561 tttggaaaag gttcaagacc tatcagcttt tgaaaaaact attcaagagg aagaaaatca
    19621 agggaagacg ttgatctttg tgtcctgtga ccatcaattg gtagcttact atgtcctatt
    19681 agatgacatc aaaatagaat cacaacgtgc tattgagacc ctccatgcca tgggaatcaa
    19741 aacagtcatg ttaacaggag atcaagaacg aaccgctaat tatgtggctc aaaaacttgg
    19801 tattgatgaa gtggtagcca actgtatgcc tcaagataag gtggctaagt tagcggaatt
    19861 aaagactaaa tatggttttg ttgccatggt aggagatggt attaatgatg cgcctgcttt
    19921 agcgcaagca gatgtgtcct atgctattgg gtcaggaaca gatattgcta tggaaagtgc
    19981 agatagtgtg atcatggacg acttgacccg tattccattt tcgattcaac tttcccgcaa
    20041 aatgaaaacc attgtcaaac aaaatatcgt tttggcctta tctgtaatta ccttattgat
    20101 tttagccaat gtttttcagg tagttaactt gccacttggt gttgttggac acgaaggctc
    20161 aacgatttta gttattttga atggcttgcg cttactttct tttaaataac ttagaaactt
    20221 gcttgatttc ttgaatacga ttataataat agtgagactt atgaaggcag aattactttt
    20281 atggttacta ttattatcgt tggtttagct atattttcaa tgatcaaaat aatgctattt
    20341 ttctattact atcgaaaatt tgagcaaaag caaaagaaga aattggattt ccaaaaccag
    20401 caaagacatt cagatgagac tactgacgtg tctccaaagg actttccatt acaaagtaaa
    20461 gaagtctagg atgatgtatc gccctctaaa gagttagaat ggttatctaa cgttaggaga
    20521 atatacctta tcattctaga cttctttttt ggtcttagtt catatagttc aactgcccac
    20581 gaaaatcttc tagggattgg tagccttttt cagtcataat cgtttttagt tccttagtga
    20641 cccgttcaaa gatagctggt ccttcctgat ggagggctgt gccaatttgg accatgcttg
    20701 ctccacaaag aatgtgctca aaagcatcac gtccagtttt gaccccacct gtaccaatga
    20761 ttttgataga aggttttaaa cgttggtaaa aggcgtggac gttggcaaga gcagtaggtt
    20821 taatgtaatc gccaccaata ccaccaaatc catttttagg cttgatgacc act
//
