LOCUS       NZ_CP047020            10384 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces broussonetiae strain T44 chromosome.
ACCESSION   NZ_CP047020
VERSION     NZ_CP047020.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces broussonetiae
  ORGANISM  Streptomyces broussonetiae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9735779)
  AUTHORS   Mo,P.
  TITLE     Streptomyces sp. strain T44 isolated from rhizosphere soil of
            Broussonetia papyrifera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9735779)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) College of Life Science and Technology,
            Central South University of Forestry and Technology, No. 498,
            Shaoshan South Road, Changsha, Hunan 410004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2019
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009796285.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:56:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,022
            CDSs (total)                      :: 8,931
            Genes (coding)                    :: 8,515
            CDSs (with protein)               :: 8,515
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 416
            CDSs (without protein)            :: 416
            Pseudo Genes (ambiguous residues) :: 0 of 416
            Pseudo Genes (frameshifted)       :: 171 of 416
            Pseudo Genes (incomplete)         :: 334 of 416
            Pseudo Genes (internal stop)      :: 29 of 416
            Pseudo Genes (multiple problems)  :: 110 of 416
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:31:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8918296
            Orig. end                         :: 8928680
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047020.1.
            Bacteria and source DNA available from Zhao YL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10384
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="32"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10384
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10384
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5384](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5384
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(361..378)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.59"
                     /tool="antismash"
     gene            1961..2797
                     /gene="fdhD"
                     /locus_tag="GQF42_RS40580"
                     /old_locus_tag="GQF42_40575"
     CDS             1961..2797
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="fdhD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037709.1"
                     /locus_tag="GQF42_RS40580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_40575"
                     /product="formate dehydrogenase accessory sulfurtransferase
                     FdhD"
                     /protein_id="WP_158928423.1"
                     /transl_table=11
                     /translation="MGRVTVRRRVLRIRDAVPSHRPDTLAAEEPMEIRVGGRPLTVTMR
                     TPGDDFDLATGFLVGEGVVHAAGDVTGIRYCAGATADGGNTYNVVDVGLAPGVSLPTAS
                     LERNFYTTSSCGLCGKASLDAVRMARRWSVAEDPLSVAPQLLAALPERLRAAQRVFDST
                     GALHAAGLFTADGELLCLREDVGRHNAVDKVVGHALRSGLLPLRNTVLLVSGRASFELV
                     QKAVLAGIPLLAAVSAPSSLAVDLAAESGLTLVGFLRGTSMNVYSGAERLAPQPVG"
     gene            complement(2814..3749)
                     /locus_tag="GQF42_RS40585"
                     /old_locus_tag="GQF42_40580"
     CDS             complement(2814..3749)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122694.1"
                     /locus_tag="GQF42_RS40585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_40580"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_158928425.1"
                     /transl_table=11
                     /translation="MLFRQLEYLVALSRERHFARAAQACYVSQPALSEALRKLEEELDV
                     PLVRRGRKYEGLTPEGERIVVWAQRILADRDALKDEVDALRGGLAGRIRIGSVPTASGA
                     VAHLTGPFCAAHPLVTVEVSADMQSVDILRQVRNFELDAGVTYLREDLADRPERFHTVP
                     LYQERYVLLLSAADALARRTTATWAQASQLPLCLLSRAMQGRRVLDELFAEAGTEASPR
                     LETDSVAALFAHVRTGQWASIVPHAWLHVFGVPHGMRAVPLVEPAASVPVGLVIAAREP
                     GSVMARALTDIARTTDVATALERIPGDSGR"
     gene            3887..4879
                     /locus_tag="GQF42_RS40590"
                     /old_locus_tag="GQF42_40585"
     CDS             3887..4879
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037710.1"
                     /locus_tag="GQF42_RS40590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_40585"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_199272969.1"
                     /transl_table=11
                     /translation="MPVVDPQPVIVPPARAAVFLVVTIAPGGEDTARGLLEDVSALRRS
                     VAFRAPAEELSCVVGIGSQAWDRLFDGPRPKQLHPFVDLAGPRHRAPGTPGDLLFHVRS
                     HRMDLCFELARLFGGRLEGAATVVDEVHGFKFFDERDLLGFVDGSENPEGRLARDSVFI
                     GDEDAEFAGGSYVIVQKYLHDMAAWNALSDEEQDRVIGRTKLANVEFPDDMKPADSHVA
                     LNTITDEDGTERKIVRENMPFGTVGKGEFGTYFIGYSRTPDVTEEMLRNMFLGREPGQH
                     DRILDFSVPVTGCLFHVPTIGFLDDLPAAPGGGSETTGGSSDSAGLKGT"
     gene            5001..5384
                     /locus_tag="GQF42_RS40595"
                     /old_locus_tag="GQF42_40590"
     CDS             5001..5384
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016349.5"
                     /locus_tag="GQF42_RS40595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_40590"
                     /product="encapsulin"
                     /protein_id="WP_233273844.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.1e-14, bitscore:
                     53.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEAPAPAVAARLRRAQHLVELRAPVTVSRDAVDDVERGARDCDRE
                     PVGHTAGAMAFAADGAVFDGCAAAGTDGLRRHTSHRVSALPGGPRTHEAAVALSAWANA
                     QGGEGGGVPAPSSVCGAVCGGVQ"
     misc_feature    5468..5482
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     gene            5475..8021
                     /locus_tag="GQF42_RS40600"
                     /old_locus_tag="GQF42_40595"
     CDS             5475..8021
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1223: ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010062665.1"
                     /locus_tag="GQF42_RS40600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_40595"
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="WP_158928427.1"
                     /transl_table=11
                     /translation="MSMSFGAPFGSSDPFSELFNRFFGMSPASSPPAVQRVPIGRLLTE
                     SSQELLNLAAQRAVEDGTTDLDTEHLLWAATKVDSSRRLLSQVGIDPDELGAEIAKILP
                     RESGEPSAQPGLTPAAKRTLGAAYAQSQAAGVSYIGPEHILSALLGDSDTGAAKLLRAE
                     GMDTDKLAGLAEQTSRTDSTAAERKQPATTLDEYGRDLTEEAKAGKLDPVVGRADEIEQ
                     TVEILSRRSKNNPVLIGEPGVGKTAIVEGLAQRIVAGEVPDTLKDKRVVSLDLSGMVAG
                     AQYRGQFEERLKKVIEDVQKAKGQIILFIDELHTVVGAGATGEGSMDAGNMLKPALARG
                     ELHVVGATTIDEYRKYVEKDAALERRFQPVMVPEPTVEETVQILEGLRDSYEAHHQVRF
                     DDAALAAAAEMSDRYITDRFLPDKAIDVMDQAGARVRLRSAGRSTEVVSREDRIAKLRR
                     ELEQAVSAEDFEKASEVKREISEVEGELAGIEERREGVVSVTAADIADVVSRRTGIPVA
                     QLTASEKEKLLRLEEEMHARIVGQEEAVTAVSQAVRRNRAGMGDPNRPVGSFLFLGPTG
                     VGKTELAKTLAALLFGEEDRMIRFDMSEFQEKHTVARLVGAPPGYVGYEEAGQLTEKVR
                     RQPYSVVLFDEVEKAHPDVFNTLLQILDDGRLTDGQGRTVDFRHCVVIMTSNIGAHRIL
                     DHKGDVSELKDELMDDLRTRFLPEFLNRIDDIIIFHGLTEDDLSRIVDHLLDQSKRRVH
                     AQGMRLEVTESAKKLLIAHGHQPEFGARPLRRTIQAELDNRIATLLLSGEAEPGDTIVA
                     DVVDDSLHCTVKKNGDSGAAEDSGDSAGAGTAAPEQ"
     gene            8035..8319
                     /locus_tag="GQF42_RS40605"
                     /old_locus_tag="GQF42_40600"
     CDS             8035..8319
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952305.1"
                     /locus_tag="GQF42_RS40605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_40600"
                     /product="hypothetical protein"
                     /protein_id="WP_158928429.1"
                     /transl_table=11
                     /translation="MARHHHKSNEQVEGNPDTGHGRGMPRRPDEPELAERTREDRREAG
                     LPTGRREDPDASYEEAQAEVDQEVREGRMKSGDTRRKDRDPYPPSDYEG"
     gene            complement(8422..8898)
                     /locus_tag="GQF42_RS40610"
                     /old_locus_tag="GQF42_40605"
     CDS             complement(8422..8898)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548779.1"
                     /locus_tag="GQF42_RS40610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_40605"
                     /product="GreA/GreB family elongation factor"
                     /protein_id="WP_158928431.1"
                     /transl_table=11
                     /translation="MTGDPEPIGAAARRALEQELSDLHDERRLVAGTMQDTTEVGDRAD
                     EADELQRADQLDRLDRRIEEITVRLRQAAPAGPAPTDAVGVGSTVTVRFPDGSVERLQI
                     GEIAEAYDQNLVTSDSPLGRALLGHHPGETVRFTAPGGPASALVVSIGEAGETG"
     misc_feature    complement(8968..8984)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.95"
                     /tool="antismash"
     gene            9010..9087
                     /locus_tag="GQF42_RS47850"
     CDS             9010..9087
                     /GO_component="GO:0005840 - ribosome [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115061593.1"
                     /locus_tag="GQF42_RS47850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="50S ribosomal protein bL37"
                     /protein_id="WP_373287922.1"
                     /transl_table=11
                     /translation="MSSKRRRKKKSRRKHAANHGKRPQS"
     misc_feature    complement(9663..9672)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 gccggctgac cgagccgatg atcaagcggc ccggcgccga gcactacgaa cccatcggct
       61 gggacgaggc gttcggggtg ctcgccgggg aactgggccg gctggactcc ccggacgagg
      121 cccttttcta cacctccggc cgggtgggca acgaggccgc gttcctcctc cagctcttcg
      181 cccgcgcctt cggcaccaac aacctgccgg actgttccaa catgtgccac gagtcgagcg
      241 gctccgcgct gacgcagacg ctcggcatcg gcaagggcag cgtctcgctg gacgacatcc
      301 acaccgccga cctggtcttc gtcgtcggac agaacccggg caccaaccac ccgcgcatgc
      361 tgtccgcgct ggaggagacc aagcggcgcg gtggcacggt cgtcgccgtc aacccgctgc
      421 ccgaggccgg actgctgcgc ttcaagcacc cgcagaaggc acgcggggtg atcggccggg
      481 gcaccgagat cgccgaccag ttcctgcaga tccgccccgg cggcgacctc gccctgttcc
      541 aggcgctcaa ccgactgctg ctggaggccg aggacgagcg ccccggcacc gtcctcgacc
      601 acgatttcat cagagcccac accaccggct tcgccgagtt cgccgaccac gcccggaaga
      661 tcgcgtggga ggacgtcctt gccgcgaccg ggctgcgccg cgaggagatc gaggccgtcc
      721 acggccgggt gctgcgcagc gggaagatca tcgtctgctg ggcgatggga ctcacccagc
      781 acaagcacgg cgtgcccacc atccgcgagg tggtcaactt cctgctgctg cgcggcaacg
      841 tcggccggcc gggcgcgggg gtgtgtccgg tgcgcggcca cagcaacgtc cagggcgacc
      901 gcaccatggg tatctgggag cggatgcccc agtcctttct cgacgccctg gaacgcgagt
      961 tcggcttcac cccgcccgcg cggcacggac tggactcggt cgacggcatc cgggccatgc
     1021 gcgacggcac cgccaaggtg ttcctcggcg tcgccggcaa cttcgtacgg gccaccccgg
     1081 acagcctggt caccgaggag gcgatgcgac ggtgccggct caccgcgcac atctccacga
     1141 agctcaaccg ctcccacacg gtctgcggcg agaccgccct catcctgccc accctcggcc
     1201 gcagcgaccg ggacatccag gcggccggtg agcagttcgt caccgtcgag gactccatga
     1261 gcgaggtgca cgcctcccgc ggcaagctgg atccggcctc cccgcatctg ctcagcgagg
     1321 tcgcgatcat cagccggctg gcccgcagga ccctcggcga caccccggac atcccctggg
     1381 aggaattcga ggccgactac ggcaccgtcc gcgaccgcat ctcccgcgtc gtccccggct
     1441 tcgaggactt caacgcccgc gtggccaggc ccggcgggtt ccggctgccc aacccggtca
     1501 acgcgcgagt cttccgcacc tcgagcggca aggccgtctt cacccgcaac gacttcacga
     1561 tgcccgacat ccccgagggg catctgctgc tccagacgct gcgctcccac gaccagtgga
     1621 acaccgttcc ctacgcgatg aacgaccgct accgcggcat ccacaacgcc cgccgtgtcg
     1681 tcctcgtcaa ccccgacgac ctcgaagcac tcgggtacgc cgaccgcgac ctggtggacc
     1741 tggtcgccgt ctggcacgac ggacgtgcgc gccgggcgga gggcttccgc gtcgtcgcct
     1801 accccaccag ccgtggctcg gccgcctcct actaccccga gacgaacgtc ctggtaccgc
     1861 tcgacagcgt cgccgacatc agcaacacgc ccacctccaa aggggtgttg gtccggctgg
     1921 agcccgcacg cgccgaggag acggggggag gtacaggacc atgggacgtg tgaccgtacg
     1981 gcggcgggtg ctgcgcatcc gcgacgccgt gccctcgcac cggccggaca ccctggccgc
     2041 cgaggaaccc atggagatcc gggtgggtgg ccgtccgctc accgtgacca tgcgcacccc
     2101 gggcgacgac ttcgacctcg cgacggggtt cctggtcggc gagggcgtcg tgcacgccgc
     2161 cggtgacgtc accgggatcc gctactgcgc gggcgccacg gcggacggcg gaaacaccta
     2221 caacgtggtc gacgtgggtc tcgcgccagg ggtgagcctg cccaccgcgt cgctggagcg
     2281 gaatttctac accacctcct cgtgcggcct gtgcggcaag gcgagcctcg acgcggtgcg
     2341 catggcgagg cgatggtccg tggccgagga tccgctgagc gtggcaccgc agctgctcgc
     2401 cgcgctgccc gagcggctgc gggcggccca gcgggtgttc gacagcaccg gcgctctgca
     2461 cgcggcaggc ctgttcacgg cggacggcga gctgctgtgc ctgcgcgagg acgtgggccg
     2521 gcacaacgct gtcgacaagg tcgtcggaca tgccctgcgc tcgggcctgc tgccgctgcg
     2581 caacaccgtg ctcctggtca gcgggcgagc ctcgttcgag ctggtgcaaa aggccgtcct
     2641 ggccggaatc cccctgctcg cggccgtctc ggcaccctcc tcgctggccg tggacctggc
     2701 ggccgagagc ggactgaccc tggtcggctt cctgcgcgga acgtcgatga acgtctacag
     2761 cggcgccgaa cgcctggcgc cccagccggt cggctgaccg ccctgcgcgc aggtcagcgg
     2821 cccgagtctc cggggatacg ctccaaagcc gtggccacat cggtcgtgcg ggcgatgtcc
     2881 gtcagggccc tggccatgac cgaccccggc tcccgcgcgg cgatcaccag gcccaccggc
     2941 accgaggccg cgggctcgac caggggcacc gcccgcatgc cgtgcggcac accgaacaca
     3001 tgcagccagg catgcggcac gatgctcgcc cactgtcccg tgcgcacatg cgcgaacaga
     3061 gcggccacgg aatcggtctc cagccggggc gaggcctcgg tccccgcctc ggcgaacagc
     3121 tcgtccagca cccggcgccc ctgcattgcc cggctcagca gacacagcgg caactgcgac
     3181 gcctgcgccc acgtggccgt cgtacgccgg gccagagcgt cggcggccga gagcagcagg
     3241 acgtaccgct cctggtacag cggcacggtg tggaaacgct ccggccgatc cgccaggtcc
     3301 tcgcgcaggt acgtcacccc cgcgtccagc tcgaagttcc gcacctgccg caggatgtcc
     3361 accgactgca tgtccgcgct gacctccacc gtgaccaggg ggtgcgccgc gcagaacggg
     3421 ccggtcagat gggcgacggc accggacgcg gtcggcaccg agccgatccg tatgcgcccg
     3481 gccagaccgc cgcgcagcgc gtcgacctcg tccttgagcg catcgcggtc ggccaggatc
     3541 cgctgcgccc acacgacgat gcgctctccc tccggggtga ggccctcgta cttgcggccg
     3601 cgtcgcacca gcggtacgtc gagttcctcc tccagcttgc gcagtgcctc cgacaaggcg
     3661 ggctgggaga cgtagcaggc ctgggcggca cgggcgaaat gccgctcgcg ggagagcgcg
     3721 acaaggtatt cgagctgacg aaagagcacg ggacgggtgt acccgacagg tccccggaat
     3781 gccaatctcc gcacgggcgc cgaactgccg agtcggccgg gcggcgccat gctggtgcga
     3841 aggagccgac ccgcatgcgg tcgtaccgga tctcaagggg aagcgtatgc ccgtggtcga
     3901 tccacagccc gtcatcgtcc cgccggcccg ggccgccgtg ttcctcgtcg tcaccatcgc
     3961 gcccggcggc gaggacacgg ctcgcggcct gctcgaagac gtctccgctc tgcgccgctc
     4021 ggtcgccttc cgcgcgcccg ccgaggaact cagctgtgtc gtaggcatcg gctcgcaggc
     4081 atgggaccgg ctcttcgacg ggccgcgtcc caagcaactg catcccttcg tggacctggc
     4141 gggcccgcgc caccgggctc ccggcacgcc cggcgacctc ctcttccacg tccggtccca
     4201 ccgcatggac ctctgcttcg agctggcccg gcttttcggg ggacgtctcg aaggagcggc
     4261 caccgtggtg gacgaggtgc acggcttcaa gttcttcgac gaacgcgatc tgctcggctt
     4321 cgtcgacggc agcgagaacc ccgagggcag gctcgcgcgg gactcggtgt tcattggcga
     4381 cgaggacgct gagttcgccg gcggcagcta tgtgatcgtg cagaagtacc tgcacgacat
     4441 ggcggcctgg aacgccctgt ccgacgagga acaggaccgg gtcatcgggc gcaccaaact
     4501 ggcgaacgtg gagttccccg acgacatgaa gccggccgac tcccatgtcg ccctcaacac
     4561 catcaccgac gaggacggca ccgaacgcaa gatcgtccgc gagaacatgc ccttcggaac
     4621 cgtcggaaag ggcgagttcg gcacctactt catcgggtac tcacgcacgc cggacgtgac
     4681 cgaggaaatg ctgaggaaca tgttcctcgg ccgggagccc ggtcagcacg accggattct
     4741 cgacttctcg gttcccgtca caggctgcct cttccatgtg ccgaccatcg gtttcctcga
     4801 cgaccttccc gccgctccgg gcggcgggag cgagacgacc ggcggatcgt cggacagcgc
     4861 aggcctcaag ggaacctgac cacgacggcg ctcaccgccg tgaccgcctt cccgcgtgat
     4921 ctcgcgggcc ggcggatcgt cgacgtcacc ggcccgacgg cccacagccg gccgcggtcg
     4981 gcacgggaca tctctccggc atcgaggcac cggcaccggc ggtcgcggcg cgcttgcgcc
     5041 gagcgcagca cctggtggaa ctgcgggcgc cggtcacggt gagccgcgac gccgtggacg
     5101 acgtcgagcg cggcgcgcgg gactgcgacc gggagccggt cgggcacacg gcaggcgcca
     5161 tggcgttcgc cgcggacggg gcggtcttcg acgggtgcgc ggcggccggg accgacgggc
     5221 tgcgccgcca tacgtcccac cgcgtgagcg ccctgcccgg cgggccccgc acccacgagg
     5281 ccgcggtggc actgagtgcg tgggcgaacg cgcagggcgg tgagggcgga ggtgtcccgg
     5341 caccgtcctc cgtttgcggt gccgtgtgcg gaggggttca gtgagtactg accgccggat
     5401 cggtcactgg cccccggatc agccccgcgt tcacaccttc gcggatcacc gtttctccgc
     5461 gcccaggagc tgtcatgtcg atgtcgttcg gagccccctt cggttcgtcg gatccgttca
     5521 gcgagttgtt caaccgcttc ttcggtatgt ctcctgcgtc atcgccgccc gcggtgcaac
     5581 gcgtgcccat cggacggctg ctgaccgagt cgtcgcagga actgctcaac ctggccgcgc
     5641 aacgggccgt cgaggacggc accacggacc tcgacaccga acacctgctc tgggcggcca
     5701 ccaaggtcga ctcgtcccgg cggctgctgt cccaggtggg catcgatccc gacgagctgg
     5761 gggccgagat cgccaagatc ctcccgcgcg agtccggcga gccgtccgcg caaccgggcc
     5821 tcacccctgc ggccaagcgc acgctcggcg ccgcctacgc acagtcgcag gcggccggtg
     5881 tgtcgtacat cggtccggaa cacatcctca gtgccctgct gggcgacagc gacaccggcg
     5941 ccgccaagct gctgcgcgcc gagggcatgg acacggacaa gctggcgggc cttgccgaac
     6001 agacctcccg caccgactcg acggccgccg agcgcaagca gccggccacg acgttggacg
     6061 agtacggccg ggacctgacg gaagaggcca aggcgggcaa gctcgacccg gtggtggggc
     6121 gcgccgatga gatcgagcag accgtcgaga tcctctcccg gcgctccaag aacaaccccg
     6181 tgctcatcgg cgaacccggc gtcggcaaga ccgccatcgt ggaggggctg gcacagcgca
     6241 tcgtggcggg cgaggtcccg gacaccctca aggacaagcg ggtggtctcc ctcgacctgt
     6301 cgggcatggt ggcgggagcg cagtaccggg ggcagttcga ggagcggctg aagaaggtca
     6361 tcgaggacgt acagaaggcc aagggccaga tcatcctgtt catcgacgaa ctgcacacgg
     6421 tcgtcggcgc cggtgcgacg ggcgaggggt ccatggacgc gggcaacatg ctcaagcccg
     6481 cactcgcacg cggtgaactg cacgtggtcg gtgccacgac catcgacgag taccgcaagt
     6541 acgtggagaa ggacgccgcg ctggagcgcc gcttccagcc ggtgatggtc cccgagccca
     6601 ccgtcgagga gaccgtgcag atcctcgagg gactccgtga ctcctacgag gcccaccacc
     6661 aggtccgctt cgacgacgcg gcgctggctg ccgccgccga gatgtccgac cgctacatca
     6721 ccgaccgttt cctgcccgac aaggccatcg acgtgatgga ccaggcgggc gcccgggtac
     6781 ggctgcgcag cgcgggccgc tccaccgagg tcgtcagccg cgaggaccgt atcgccaagc
     6841 tgcgccggga actcgaacag gccgtctccg ccgaggactt cgagaaggcc tccgaggtga
     6901 agcgggagat ctccgaggtg gagggcgaac tcgccgggat cgaggagcgt cgcgagggcg
     6961 tcgtgtcggt cacggccgcc gacatcgcgg acgtcgtctc ccgccgaacc ggcatcccgg
     7021 tcgcccagct caccgccagc gagaaggaga aactcctcag gctggaggag gagatgcacg
     7081 cccggatcgt cggccaggag gaggcggtca ccgcggtctc gcaggccgtg cgccgcaacc
     7141 gcgcgggcat gggcgacccc aaccggcccg tgggctcgtt cctcttcctc ggccccaccg
     7201 gcgtcggcaa gaccgaactc gccaagacac tcgcggcgtt gctgttcggc gaagaggacc
     7261 gcatgatccg cttcgacatg agcgagttcc aggagaagca cacggtcgca cggctcgtcg
     7321 gggccccgcc cggatacgtc ggctacgagg aggcgggcca gctgaccgag aaggtgcgcc
     7381 gccagccgta cagcgtggtg ctgttcgacg aggtggagaa ggcccacccc gacgtgttca
     7441 acaccctgct gcagatcctg gacgacggcc ggctgaccga cgggcagggc cgcaccgtcg
     7501 acttccggca ctgcgtcgtc atcatgacgt ccaacatcgg tgcgcaccgc atcctcgacc
     7561 acaagggcga tgtgtccgag ctgaaggacg agttgatgga cgacctgcgg acgaggttcc
     7621 tgccggagtt cctcaaccgc atcgacgaca tcatcatctt ccacggtctg accgaggacg
     7681 acctgagccg gatcgtggac cacctgctgg accagagcaa gcgccgggtg cacgcgcagg
     7741 gcatgcggct cgaggtcacc gagtccgcga agaagctgct catcgcacac ggccaccagc
     7801 cggagttcgg cgcccgtccg ctgcgccgca ccatccaggc ggaactcgac aaccgcatcg
     7861 ccaccctcct gctgagcggc gaggcagagc ccggggacac gatcgtcgcc gacgtggtgg
     7921 acgactcgct gcactgcacg gtgaagaaga acggcgactc cggcgcagcc gaggactccg
     7981 gcgactccgc cggggccggc accgcggcgc cggagcagtg acggaggagg acccatggcg
     8041 cgtcaccacc acaagtccaa cgagcaggtc gagggcaatc cggacaccgg tcacggtcgc
     8101 ggtatgccgc gccggccgga cgagccggaa ctcgccgagc gtacgcgcga ggaccggcgg
     8161 gaagcgggtc tgccgaccgg gcgccgggag gaccccgacg cctcgtacga ggaggcacag
     8221 gccgaggtcg accaggaggt gcgcgagggc cggatgaagt ccggcgacac ccggcgcaag
     8281 gaccgcgacc cgtacccgcc gagtgactac gagggctgac acacccccgc cacgcccgca
     8341 tcgtgagaac cggttccggc ggccaccggt caaccatggc cgccgggagg gccgttcggc
     8401 ctgtcgcccg gcctcagcct gtcacccggt ttcgcccgcc tcgccgatcg acaccacgag
     8461 cgcactcgcc ggaccgccgg gtgcggtgaa gcggacggtt tcaccgggat ggtggccgag
     8521 cagggcacgg ccgagtggac tgtccgaggt gaccaggttc tggtcgtacg cctcggcgat
     8581 ctcgccgatc tggagcctct ccaccgagcc gtccgggaag cggacggtga cggtgctgcc
     8641 cacgcccacc gcgtcggtgg gggcgggccc ggccggcgcg gcctggcgca gccgtaccgt
     8701 gatctcctcg atacggcggt ccaggcggtc gagttggtcg gcccgctgca actcgtcggc
     8761 ctcgtcggcg cggtcgccca cctcggtggt gtcctgcatg gttccggcga cgagccgccg
     8821 ctcgtcgtgc aggtcggaca gctcctgctc cagcgcacgg cgggccgcgg cgccgatcgg
     8881 ctcggggtca ccggtcattt cggctcctca ggtcgtatag ggcgaaaatg atccaagatg
     8941 cgagcaaaga taatgaggcg gatatttttc gtatcgtata tcgaatcatt cccttgtggg
     9001 gaggtcgcca tgtcctcgaa gcgacgcagg aagaagaagt cgcgacgcaa gcacgccgcc
     9061 aaccacggga agcggcctca gtcctgagcc gggccggcac cccgggcccg cgcacccgga
     9121 cggcggaagg ggagagccgt gagcacctcg cgtgacgagc agcagcgcag gaccgccgag
     9181 ggggagcaag ggccggagcc ccgcgtgggc cccgcccccg ggggtgagcc gtccttccgc
     9241 ccctaccacc accccgccgc cggctggggc gccgcgaaga gcgtgagcag gttcctggcg
     9301 cgcgagggtg ccctggtcga cggaccgcgc gcgatcgtga ggatgaacca cgagaacgcg
     9361 ggcttcgact gccccgggtg cgcatggccg gacgacacca agggcctgcg cctggacatc
     9421 tgcgagaacg ggatcaagca cgtcacctgg gagatgaccc gaaagcgggt cggccgcgcg
     9481 ttcttcgcgg agcactcggt gagcgaactg tccgaatgga gcgacttcgc cctggaggac
     9541 cagggccggc tcaccgagcc catggtctac gaccccggcc gcgaccacta cgtgccgatc
     9601 agctggcgcg acgccttcga gctggtcggc cggaccctgc gcgagctgga cgcccccggc
     9661 caggccgcct tctacacctc cggacggctc ggcaacgagg ccaccttcct gtaccagctg
     9721 atggcccggg aactgggcac caacaacctc cccgactgct ccaacatgtg ccacgaggcc
     9781 agcggacgcg ccctcgaggc ttcgctcggc accggcaagg gcacggtcga cctcaaggac
     9841 tggcagagca cagacgccct gttcatcatg ggcgtgaacg ccgcctccaa cgcgccccgg
     9901 atgctcaccg ccctcgccga ggcctaccgg cgcggcgccc agatcgtgca cgtcaacccg
     9961 ctgatcgagg cggccgcccg gcgcacgatc atcccgcacg acttcgtgga catggccacc
    10021 ggccactcca cccgcaccag caccctgaat ctccaggtgc gcatcggcgg ggacatggcg
    10081 ctgctgcgcg gcatggcgaa ggccgtgctg gaacaggcca ggagcgaccc caaggccctg
    10141 gaccaggagt tcatcgaacg tcacaccatc ggcttcgacg actaccggaa ggtgtgcgag
    10201 gccacgccct ggcaggagat cgagcgccag tcgggcctcg accgcgccga catcctcaag
    10261 gccgcccgcg tctaccgcga cgccgaccgc agcatcgtca gctggtgcct gggcctcagc
    10321 cagcacgagc acggcgtcga caccgtccgc gagatcgtca acctgcttct gctgcgcggc
    10381 aatc
//
