LOCUS       NZ_CP047020            11323 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces broussonetiae strain T44 chromosome.
ACCESSION   NZ_CP047020
VERSION     NZ_CP047020.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces broussonetiae
  ORGANISM  Streptomyces broussonetiae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9735779)
  AUTHORS   Mo,P.
  TITLE     Streptomyces sp. strain T44 isolated from rhizosphere soil of
            Broussonetia papyrifera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9735779)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) College of Life Science and Technology,
            Central South University of Forestry and Technology, No. 498,
            Shaoshan South Road, Changsha, Hunan 410004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2019
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009796285.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:56:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,022
            CDSs (total)                      :: 8,931
            Genes (coding)                    :: 8,515
            CDSs (with protein)               :: 8,515
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 416
            CDSs (without protein)            :: 416
            Pseudo Genes (ambiguous residues) :: 0 of 416
            Pseudo Genes (frameshifted)       :: 171 of 416
            Pseudo Genes (incomplete)         :: 334 of 416
            Pseudo Genes (internal stop)      :: 29 of 416
            Pseudo Genes (multiple problems)  :: 110 of 416
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:31:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7099274
            Orig. end                         :: 7110597
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047020.1.
            Bacteria and source DNA available from Zhao YL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11323
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="21"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11323
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11323
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6323](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6323)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            332..1063
                     /gene="hemQ"
                     /locus_tag="GQF42_RS32660"
                     /old_locus_tag="GQF42_32660"
     CDS             332..1063
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="GQF42_RS32660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_32660"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_158925916.1"
                     /transl_table=11
                     /translation="MSDDAPTTEPGRIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDVSGLRADADLMIWWHAETSDQLQEAYNLFRRTK
                     LGRALTPVWSNMALHRPAEFNRSHIPAFLADETPRNYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGFPDVRANTVASFSLGDYEWVLAFEADELYRIVDLMRHLRGSEARKHVR
                     EEIPFYTGRRKDIADLVAGLA"
     gene            complement(1156..2814)
                     /locus_tag="GQF42_RS32665"
                     /old_locus_tag="GQF42_32665"
     CDS             complement(1156..2814)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (117-487). E-value:
                     4.3e-17. Score: 54.5. Matches aSDomain:
                     nrpspksdomains_GQF42_RS32665_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432060.1"
                     /locus_tag="GQF42_RS32665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_32665"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_158925918.1"
                     /sec_met_domain="PF00561 (E-value: 3.7e-20, bitscore: 71.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYSAAGSLFLTTLAAVPAGSADAAPGAPGVPGFAELRGTA
                     VAAARARAAGIRFGACSPADVPKAPRSLRCGTVTVPLDYAHPDGARIKLTVSRTRATQK
                     DPQNSKQRVPRQGALVYNPGGPGGSSMIFPLIGNTPKWKRIAAAYDLVGYAPRGVGRSA
                     PLSCADPRHLSTGPTAAPEHPSQAYKLRRIAQAKAYARGCAQRSGSKLRFYTSLNNARD
                     LDVLRAALGEDRLTFVGASYGTYFGALYAAMFPSHLRRMVLDSSVNPAPEKIWYRSNLD
                     QSAAFEERWSDLKDWIAHHDDVYRLGNTAAKVQRSYDTARRRLARKAAGGKVGPGQLQN
                     ALLTAGYYDGFWPSRAAALSAYLKGDPKPLIQLAAPATDRAAAAEAENSDAVYTAVECN
                     DAPWPTDWKTWDRDNTRIARAAPFETWNNAWMNLPCAYWPAPRQQPLDVRTGPGEVPPV
                     LILAAERDAAAPYSGALEMNRRLAGSVLVTERDAGTHGIGGGANACVNDHFEAYLVQGR
                     VPGRRASCAPHRLPAAVRPTKESQRDSVKGRGGRH"
     aSDomain        complement(1354..2463)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GQF42_RS32665_Abhydrolase_1.1"
                     /evalue="4.30E-17"
                     /label="GQF42_RS32665_Abhydrolase_1.1"
                     /locus_tag="GQF42_RS32665"
                     /protein_end="487"
                     /protein_start="117"
                     /score="54.5"
                     /tool="antismash"
                     /translation="ALVYNPGGPGGSSMIFPLIGNTPKWKRIAAAYDLVGYAPRGVGRS
                     APLSCADPRHLSTGPTAAPEHPSQAYKLRRIAQAKAYARGCAQRSGSKLRFYTSLNNAR
                     DLDVLRAALGEDRLTFVGASYGTYFGALYAAMFPSHLRRMVLDSSVNPAPEKIWYRSNL
                     DQSAAFEERWSDLKDWIAHHDDVYRLGNTAAKVQRSYDTARRRLARKAAGGKVGPGQLQ
                     NALLTAGYYDGFWPSRAAALSAYLKGDPKPLIQLAAPATDRAAAAEAENSDAVYTAVEC
                     NDAPWPTDWKTWDRDNTRIARAAPFETWNNAWMNLPCAYWPAPRQQPLDVRTGPGEVPP
                     VLILAAERDAAAPYSGALEMNRRLAGSVLV"
     gene            complement(2964..3752)
                     /locus_tag="GQF42_RS32670"
                     /old_locus_tag="GQF42_32670"
     CDS             complement(2964..3752)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675656.1"
                     /locus_tag="GQF42_RS32670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_32670"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_158925920.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWAFAGTACTRLCLSAVHAATPDTHDTDTTTVDRGH
                     DLTLYEAAFLSGGPARVADVAMISMARQRRLLLAHTGWATVVDPRGRDEMEQCVIGAIG
                     PQGQSRIQPVRARAAGGEAVRRLADRLERAGLAVPDGAGTTVAAAVRQVRAAALAAAVL
                     GVTALLLPIQTDTPRSLVGLWFALPLTLSLSCLAIARFEVHPYSRWASPAGLRLLGALP
                     RKPAGDERSFLASVAVRGIRAIGEPELRAAFTHRDQPWRE"
     misc_feature    3755..3764
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.4"
                     /tool="antismash"
     gene            complement(3867..4631)
                     /locus_tag="GQF42_RS32675"
                     /old_locus_tag="GQF42_32675"
     CDS             complement(3867..4631)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125239.1"
                     /locus_tag="GQF42_RS32675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_32675"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_158925922.1"
                     /transl_table=11
                     /translation="MRPRPPIKGRGIFSGTGLVIAALAVTAAALLYPLWSYADRQDTAD
                     AGVLSAQAVATPYGPLSAQDQDFITRVRLAGLWELPAGQLAERKGTTAAVRTAGQHLVD
                     GHTSLDAHVRTVATQLGVPLPNEPNAQQKQWLATLNTAQGEDFDRTFAAILRLAHGRVF
                     TLVAQVRSDTQNSLVRDLADDANATVLDHIKVLEATGYVDFATLARDLAASPTPVPTPP
                     AVAPSAAVPVTPSPAESYSLPPAAASPAPSTP"
     gene            complement(5001..6323)
                     /locus_tag="GQF42_RS32680"
                     /old_locus_tag="GQF42_32680"
     CDS             complement(5001..6323)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643869.1"
                     /locus_tag="GQF42_RS32680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_32680"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_158925924.1"
                     /sec_met_domain="DUF692 (E-value: 1.8e-94, bitscore: 314.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIADAVERMPGIDWVEVVAENVCPGHLPESLLR
                     LRECGVTVIPHGVSLGLGGADRPDEGRLTALAERAQALGSPLVTEHIAFVRAGGALTAS
                     PPLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALFSWPGEELTEGQFLS
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAEALAALPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDILTDLAARTAPPGVLLERDDNFPEPAQLERELALIQEAVATGGAAVGGG
                     TGATVGTGPADGGVAVLTRPAVGARERVGVAQAAVLSSLVAGTPVPEGFDRARMRVQAR
                     ALAAKRADVVAKVAPELPVILGAGYRALFLAYARERPMSGGYRRDALDFAGLLLRSGRP
                     ERAEARRELRQWWLDRTGPAPRPTGGLVARTARRVLSGRLD"
     gene            complement(6596..7060)
                     /locus_tag="GQF42_RS47335"
     CDS             complement(6596..7060)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012363.5"
                     /locus_tag="GQF42_RS47335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carboxylesterase family protein"
                     /protein_id="WP_325100367.1"
                     /transl_table=11
                     /translation="MRHCRAARPAAFPEASPGELLVQIATDWFYRLPALRLAEAQAQAQ
                     ARHGARAYVYEFAWPSPAFEGTLGACHVLDVPFVFDNPADPAFTPLLGDFPPQAIADSM
                     HAAWVSFATTGDPGRPAYREPHRTVRRFADAPATDHDPRGSLRTLWDGLR"
     gene            complement(<7095..7478)
                     /locus_tag="GQF42_RS47340"
                     /pseudo=""
     CDS             complement(<7095..7478)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012363.5"
                     /locus_tag="GQF42_RS47340"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="carboxylesterase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDRLLPERIVPGEDFLNLNVWTPDPAGRLPVLVWLHGGAFTNGSG
                     SLSGYDGAAFARDAVVLVTLNHRLGVEGFLHLGPGDTAPVNLGLLDQIAALAWVRDNIA
                     AFGGDPDRVTVFGQSAGAMSVGTL"
     gene            complement(<7568..7684)
                     /locus_tag="GQF42_RS47345"
                     /pseudo=""
     CDS             complement(<7568..7684)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012363.5"
                     /locus_tag="GQF42_RS47345"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRPVLATRHGLARGVTGPAGTACFRNVPYAAAPTGPLRC"
     gene            complement(7728..8375)
                     /locus_tag="GQF42_RS32690"
                     /old_locus_tag="GQF42_32690"
     CDS             complement(7728..8375)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF036525.5"
                     /locus_tag="GQF42_RS32690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_32690"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_199272879.1"
                     /transl_table=11
                     /translation="MELPAWEPRRVVAMEAVLPEGVTPPGTRGKILEAGLELFAESGFA
                     GTSIRQIAGKVGINSATLYAHYPSKGHVLAELVRIGHEEMRTRLAQAAAETRDTGAARQ
                     LAALVRAHVLLHTDHPLLAVVTNGELHALSPELATPSLQLREECRGMLADVLERGIGNG
                     EFELDDPLLAVTAIGGLGVQVAQWFGPALGRTREQIADQYARFALRIVNAGR"
     misc_feature    8414..8427
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.88"
                     /tool="antismash"
     misc_feature    complement(8414..8428)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.06"
                     /tool="antismash"
     misc_feature    8415..8428
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.26"
                     /tool="antismash"
     misc_feature    8415..8429
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.22"
                     /tool="antismash"
     misc_feature    complement(8478..8487)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.27"
                     /tool="antismash"
     gene            8562..9767
                     /locus_tag="GQF42_RS32695"
                     /old_locus_tag="GQF42_32695"
     CDS             8562..9767
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021982.5"
                     /locus_tag="GQF42_RS32695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_32695"
                     /product="PHB depolymerase family esterase"
                     /protein_id="WP_158925926.1"
                     /transl_table=11
                     /translation="MRFRSPRRAAQALAALGAALALAAGGPAASARAAAPPLPDLNITG
                     TYVTGVSSGGFMATQLQVAYSGTFDGAGIVAAGPYDCGQGHVIEFVTCDLGLGVGDLER
                     QAEDWASEGRIDPVANLRGKPVYAYHGTLDPVVNPLVSRAGVDFYEHFGADVTYHDRDP
                     AGHSWPTPDGVVPCALTLPPFLNNCHDDPEQEMLTAWLGGVNPPNTGAPRGTLTYFDQN
                     AYAPGGSARALSMDSTGQLYLPPACANGAPCKLVVALHGCLSAQQLLGTAFAEKSNIDT
                     YADTNNLVVLYPQAVSSVWPVNPQGCWDWFGYTGSDYAVKSAPQMTAVVNMVHALGARP
                     APAVPAVPVPAVPAVRGPGTAGPGPLRRRERRSGGPLTDVRGPAPPPAHAGPSWSGRGP
                     RW"
     gene            complement(10490..11185)
                     /locus_tag="GQF42_RS32700"
                     /old_locus_tag="GQF42_32700"
     CDS             complement(10490..11185)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993753.1"
                     /locus_tag="GQF42_RS32700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_32700"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_158930839.1"
                     /transl_table=11
                     /translation="MPPSDSRDEAPQFVLPLVVRIERAAPPARTDALETAARAVLVLLA
                     DERAAGDGEWAEAVRNWEDARIRKVVRRARGAEWRRAEALPGITVTGKAAQVRVFPPVP
                     LDGWPKDLAKLQVSGTELDDPEPPVAAESAQPVLWLNPALEMSAGKTMAQAGHGAQLAW
                     WALPAAERTAWRDTGFALCVRTADPADWARLTGSGLPLVRDAGFTEIAPGSCTVVADHP
                     ALRQGVSDG"
     misc_feature    11268..11283
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.31"
                     /tool="antismash"
     misc_feature    11295..11305
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
ORIGIN
        1 gacgccgccc tggtcgaggt ctcccggcac gacctgcgcg aggccaccgg cctggacgcc
       61 cgccccctcg agacgcgggt cacccgctgg agcgacggcc tgccgcagta cccggtcggg
      121 caccacgcgc gcgtggcccg catccgcgaa cacgtcgccg ggctgccggg cctggcggtg
      181 tgcggcgccc agtacgacgg cgtcggcatc ccggcgtgca tcgcgagcgc ctatgcggcc
      241 gtcgaccagc tccacggcga cctgagcgga atccgggagc tgacggccaa cccggtgcaa
      301 agcctccacg gcggagcggg agaataggac catgagcgac gacgccccca ccaccgagcc
      361 cggcaggatc ccgaacaagg gcaagctggc caaggacctc aacgaggtca tccgttacac
      421 cctgtggtcg gtcttcaagc tgaaggacgt actgcccgag gaccgcgcgg gttacgccga
      481 cgaggtccag gagctgttcg accagctcgc cgccaaggac gtgacgatcc gcggcaccta
      541 cgacgtgtcc ggcctgcgcg ccgatgccga cctcatgatc tggtggcacg ccgagaccag
      601 cgaccagctc caggaggcgt acaacctctt ccgtcgcacg aagctgggcc gcgccctgac
      661 gcccgtgtgg tccaacatgg cgctgcaccg cccggccgag ttcaaccgct cgcacatccc
      721 ggcgttcctc gccgacgaga cgccccgcaa ctacgtcagc gtctacccct tcgtgcgctc
      781 ctacgactgg tacctgctgc ccgacgagga ccgccgccgc atgctcgccg accacggcaa
      841 gatggcccgc ggcttccccg acgtgcgcgc caacacggtc gcctcgttct ccctcggcga
      901 ctacgagtgg gtcctcgcct tcgaggccga cgagctgtac cgcatcgtcg acctcatgcg
      961 tcacctgcgc ggttccgagg cccgcaagca cgtccgcgag gagatcccgt tctacacggg
     1021 ccgccgcaag gacatcgcgg acctggtggc cgggctggcc tgaagcgccc cgtcaggggg
     1081 cgcggggcac cgcgcgagcg accacgacgg acccgcagcc gcccggcggc acagcgcggc
     1141 acgacaatgg cgcactcagt gccgtccgcc ccggcccttg accgagtccc gctgagactc
     1201 cttcgtcggc cgaaccgccg caggcaaccg gtgcggcgcg cacgacgcgc gccgcccggg
     1261 gacccggccc tgcaccaggt acgcctcgaa gtggtcgttg acgcacgcgt tggccccgcc
     1321 cccgatgccg tgcgtgcccg cgtcccgctc ggtcaccagc accgagccgg ccagccgccg
     1381 gttcatctcc agtgcacccg agtacggtgc cgccgcgtcc cgttcggccg ccaggatcag
     1441 caccggcggc acctcacccg gccccgtccg cacgtccagc ggctgctgcc ggggcgccgg
     1501 ccagtaggcg cacggcaggt tcatccacgc gttgttccag gtctcgaagg gcgcggcccg
     1561 ggcgatccgg gtgttgtccc ggtcccacgt cttccagtcc gtcggccagg gcgcgtcgtt
     1621 gcactccacg gccgtgtaca ccgcgtcgct gttctccgcc tccgctgccg cggcccggtc
     1681 cgtggccggt gcggccaact ggatcagtgg cttgggatcg cccttgagat aggccgagag
     1741 cgcggcggcc cggctcggcc agaagccgtc gtagtacccg gccgtcagga gcgcgttctg
     1801 cagctggccg gggccgacct tcccgccggc cgccttcctg gccagccgcc tgcgcgcggt
     1861 gtcgtagctg cgctgcacct tcgcggccgt gttcccgagc cggtagacgt cgtcgtggtg
     1921 ggcgatccag tccttcagat ccgaccagcg ctcctcgaac gcggccgact ggtccagatt
     1981 ggaccggtac cagatcttct ccggggccgg gttcaccgac gagtccagca ccatgcgccg
     2041 caggtgcgag gggaacatcg ctgcgtacag cgccccgaag taggtgccgt acgacgcgcc
     2101 cacgaaggtc agccggtcct cacccagcgc ggcccgcagg acgtccaggt cccgggcgtt
     2161 gttcagcgag gtgtagaagc gcagcttgct cccggagcgc tgggcgcagc cgcgcgcgta
     2221 cgccttcgcc tgcgcgatgc gccggagctt gtacgcctgc gaagggtgct ccggtgccgc
     2281 ggtgggccct gtggagaggt gcctggggtc cgcacaggac agcggcgccg agcggcccac
     2341 cccgcgcggg gcgtagccga cgaggtcgta ggcggcggcg atccgcttcc acttgggggt
     2401 gttgccgatc agcgggaaga tcatgctgga gccgccggga ccgccggggt tgtagacgag
     2461 ggcaccctgc cggggcacgc gctgcttgct gttctgcggg tccttctggg ttgcccgggt
     2521 ccggctgacg gtcagcttga tccgggcgcc gtccgggtgg gcgtagtcga gcgggacggt
     2581 caccgtgccg caccgcaggc tcctcggcgc cttgggcacg tcggcggggg agcacgcgcc
     2641 gaaacggatg cccgccgcgc gggcgcgggc cgcggccacc gcggtgccgc gcagctcggc
     2701 aaagccgggc accccgggcg ccccgggcgc ggcgtcggcg cttccggccg ggacggcggc
     2761 cagggtcgtc aggaacaagg acccggcggc cgagtacagg gcggcagctc tcatcgcgtc
     2821 tcccttcggt gccgcggcgg cgatggcacg gctgccacgc cccgacgaca acaaaaggga
     2881 tatttcgttc ggcggtgagg gaaggcaagc accacccggc cggtgtcggc ggcattgccc
     2941 ccgatgcgcc cctcgggtgg ccatcactcc cgccagggct ggtcgcggtg ggtgaacgcc
     3001 gcacgcagct ccggctcgcc gatcgcgcgg atgccccgga cggcgacaga ggcgaggaac
     3061 gaccgctcgt ccccggccgg tttgcggggc agcgcgccga gcaggcgcag cccggccgga
     3121 gaggcccagc gggagtacgg gtgcacctcg aaccgggcga tggccaggca gctcagggac
     3181 agtgtgagag gcagggcgaa ccacaggccg accagtgacc ggggggtgtc ggtctggatg
     3241 ggcagcagca gtgcggtcac cccgagcacc gccgcggcca gtgccgcggc gcgcacctgg
     3301 cggacggccg ccgccactgt cgtaccggcg ccgtcgggga cggcgagccc ggcgcgctcc
     3361 agccggtcgg cgagccgccg tacggcctcg ccgccggccg cgcgcgcccg tacgggctgg
     3421 atccgcgact ggccctgcgg ccctatggcc ccgatgacgc actgctccat ctcgtcgcgc
     3481 ccccgcgggt ccaccaccgt ggcccagccg gtgtgcgcga gcagcaggcg gcgctggcgg
     3541 gccatcgaga tcatggccac gtcggcgacc cgggcgggac cgccggagag gaaggccgcc
     3601 tcgtacagcg tcagatcgtg cccccggtcc actgtcgtcg tgtccgtgtc atgcgtgtcc
     3661 ggcgttgccg catggacggc ggacagacac agccgggtgc acgccgtacc ggcgaaagcc
     3721 caggccagga gcagcagtag gacccagaac atgacgtttt tctatgccag aggtccgggt
     3781 gggcgccatg ccttgttcac catccggacg gagtgtggcc ggtatatgac gttccgtgtc
     3841 ttgttgagat cgccaagtcg acgaggtcag ggggtgctcg gcgccgggct cgcggcggcg
     3901 ggcggcaggg agtacgactc ggcgggcgac ggtgtcaccg ggaccgcggc gctcggggcg
     3961 acggccggcg gggtcggcac gggggtgggc gaggcggcca ggtccctcgc cagtgtggcg
     4021 aagtccacgt atcccgtcgc ctccaggacc ttgatgtgat cgaggacggt cgcgttggcg
     4081 tcgtcggcga ggtcgcggac cagggagttc tgggtgtcgg accggacctg ggcgaccagc
     4141 gtgaacaccc ggccgtgcgc gagccgcagg atggcggcga acgtgcggtc gaagtcctct
     4201 ccctgcgccg tgttcagggt ggcgagccac tgcttctgct gtgcgttcgg ctcgttcggc
     4261 agggggacgc cgagctgggt ggcgacggtg cggacatgcg cgtccagcga ggtgtgaccg
     4321 tcgacgaggt gctggcccgc ggtgcgtacg gcggccgtgg tccccttgcg ctccgcgagc
     4381 tggccggcgg gcagctccca gagccccgcg agccggaccc tggtgatgaa gtcctggtcc
     4441 tgcgccgaca gcgggccgta cggagtcgcg acggcctgtg cgctcagcac gccggcgtcg
     4501 gccgtgtcct gccggtcggc gtacgaccac agcgggtaca gcagtgccgc cgcggtcacc
     4561 gcgagcgccg cgatgacgag cccggtgccg ctgaatatgc ctcggccctt gatcgggggg
     4621 cggggtcgca tggcgcctcc tgtagcgcac cgcacgcgag tgtgcctggg ggtgggtgcg
     4681 gtttggcata ttactggcgc cgcgcatccc tgagcgcgtg gcgaggggca cagggccgcc
     4741 gtcccgcccc cgcggaccgg cgacccgggg gtgtgctgtg acgaaggtgg caccggaact
     4801 gccggagggc cttggcggcg gtcctttcgg ccgccactcc cggcggcgcg ccgtcccgct
     4861 ccggcgcgtc ggtgccggcg gccatgttgt ggccggcggt gccgaccgtg ccgggcggcg
     4921 gggcggcgca tcggtacggc tgcgcgcccc ttcagggtgc ctgcgcccgg cgggcgcagg
     4981 tgggtgactt caccgtcgga tcagtccagg cgccctgaca ggactctgcg ggccgtgcgg
     5041 gccaccagcc cgccggtcgg cctcggtgcc ggtcctgtcc ggtccagcca ccactgccgt
     5101 agctcccgcc gcgcctccgc ccgctccggc cgcccggacc gcagcagcag gccggcgaaa
     5161 tccagcgcgt cccgccggta ccctccgctc atcggccgct cccgtgcgta ggcgaggaac
     5221 agcgcccggt agccggcgcc gaggatcacc ggcagttccg gtgccacctt cgccaccaca
     5281 tccgcccgct tggccgcgag ggcgcgggcc tgcacccgca tccgcgcccg gtcgaacccc
     5341 tcgggtaccg gcgtcccggc caccagcgag gacagcacgg cggcctgggc gacgccgacc
     5401 cgctcccgcg cgccgaccgc cggccgcgtg agcacggcga cacccccgtc cgccggcccg
     5461 gtgccgacgg tggcccccgt accgccgccg acagcggcgc cgccggtcgc caccgcctcc
     5521 tggatcaggg ccaactcccg ttccagctgg gccggttcgg ggaagttgtc gtcccgttcc
     5581 agcagtaccc cgggcggggc ggtacgggcg gcgaggtcgg tcaggatgtc gaggaccggg
     5641 cgcggcacgg gatgggcgtg gctgtcgtgc cagacgccgt cccgctcgaa gccgcccgcc
     5701 acatggacgt aggcgatcgc ctccagaggc agcgcggcca gtgcctcggc ggggtcctcg
     5761 ccccggttga cgtggttggt gtgcagattg gccacgtcga tcagcagccg tacgccggtg
     5821 cgctcgacca gctcggacag gaactgcccc tcggtcagct cctcgcccgg ccaggagaac
     5881 agcgcggcga tgttctccag ggccagcggc acgggcaggg cgtcctgcgc gatacggacg
     5941 ttctcgcaga gcacgtccag ggcgtccctg gtgcgcggca ccggcagcag atgcccggcc
     6001 tcaagcggcg gggaggcggt gagcgcaccg cccgcgcgga cgaacgcgat gtgctcggtg
     6061 accagggggg aaccgagggc ctgcgcgcgt tcagccagcg ccgtcaagcg gccctcgtcg
     6121 gggcggtcgg caccgcccag gccgagggag accccgtgcg ggatcacggt caccccgcac
     6181 tcgcgcaacc gcagcagcga ctccgggagg tgccccgggc agacgttctc cgccacgacc
     6241 tccacccagt cgatccccgg catccgctcc acggcgtccg cgatctccgg ccgccagccg
     6301 atccccgtgc ccagtcgccg catgagtcct cccctccgtc gaatgagggg ggtatggccc
     6361 cggcgggcgg agccgaatcc cctgcctgtc gcgttcagag caacatttga ggttcagccc
     6421 agtcccggcc accactgcgg gctacggtcc ggcagacccg gcagacccgg cagacccggc
     6481 agacccggca gacccggcag acccggcagg cccggcggac gcgggccact gcttggcccg
     6541 ggaggccctg ccccacgccg acaaccccag caggccgcac gcaaggagca cgtcgtcagc
     6601 gcaggccgtc ccacagggtc cgcagactgc cgcgtgggtc gtggtcggtg gccggcgcgt
     6661 ccgcgaaccg gcgtacggtc cggtgcggtt cacggtaggc gggccggccc ggatcgccgg
     6721 tggtggcgaa ggagacccag gcggcgtgca tgctgtcggc gatcgcctgg ggcgggaagt
     6781 ctccgagcag gggcgtaaag gccgggtcgg ccgggttgtc gaagacgaag gggacgtcga
     6841 gcacgtggca cgcgcccagc gtgccctcga aggcggggga gggccaggcg aactcgtaga
     6901 cgtaggcgcg agcaccatgg cgggcctggg cctgggcctg ggcctcggcg aggcgcagcg
     6961 cggggaggcg gtagaaccag tcggtggcga tctgtacgag caactcgccc gggctcgctt
     7021 cggggaacgc ggcgggccgc gccgctctgc agtgccgcac ggtggaagag gcccttggcc
     7081 gcgggcatgg ccagcagggt gccgacgctc atcgccccgg ccgactggcc gaacaccgtg
     7141 acgcgatcgg ggtcaccgcc gaaggcggcg atgttgtcgc ggacccatgc gagcgcggcg
     7201 atctggtcca gcagtccgag gttcaccggt gcggtgtcgc cggggccgag gtgcaggaaa
     7261 ccctccacgc ccagacggtg gttgagggtg acgagaacga ccgcgtcgcg ggcgaacgcg
     7321 gcgccgtcgt agccggagag ggaaccggag ccgttggtga acgcgccgcc gtggagccag
     7381 accaggacgg gcaggcgccc ggccggatcg ggggtccaga cgttgaggtt gaggaagtcc
     7441 tcgccgggga cgatgcgctc ggggagcagg cggtccatcg gcggcggata cggtgcctgc
     7501 ggggcggtgg accgtaggca ccggcgtcac ggaccccgtc ccacgcggcg ggcgaccgcg
     7561 gcggtgcgca gcgcagcggg ccggtggggg cggcggcgta cgggacgttc ctgaaacagg
     7621 cggttccggc agggccggtg acgccgcggg cgaggccgtg ccgggtggcg aggaccggtc
     7681 gcatgggcac tccttgcgca cagagcgggg acggggcacc ggtcggctca gcggccggcg
     7741 ttcacgatgc gcagcgcgaa ccgcgcgtac tggtcggcga tctgttcgcg ggtccggccg
     7801 agggcggggc cgaaccattg ggcgacctgc acaccgaggc cgccgatcgc ggtgaccgcc
     7861 agcagcggat cgtcgagctc gaactcgccg ttgccgatgc cgcgttccag gacgtcggcg
     7921 agcatgccgc ggcactcctc gcgcaactgg agcgagggcg tggccagttc gggcgacagg
     7981 gcgtgcagtt cgccgttggt gaccaccgca agcaggggat ggtcggtgtg gagcagtaca
     8041 tgggcgcgga ccagggcggc cagctgccgg gccgcgccgg tgtcgcgggt ttcggcggcg
     8101 gcctgcgcca gccgggtccg catctcctcg tggccgatgc gtaccagctc ggccaggacg
     8161 tgccccttgg acgggtagtg cgcgtacagg gtcgcggagt tgatgccgac cttgcccgcg
     8221 atctgccgga tcgaggtgcc cgcgaagccg gactcggcga acagctccag gcccgcctcg
     8281 aggatcttgc ctcgggttcc gggcggcgtg acgccctcgg ggagtaccgc ctccatggcg
     8341 acgacccggc ggggttccca ggcggggagt tccacggctg ctcctgaagg ctcggcgggg
     8401 tgccaactcg gcgaattttc caaccaatcg attggttgac accgtaacgc agaagccgga
     8461 tggtggctca cgacgcgtca gaaaacaacc aatcgattgg ttggaatcag cagcctggtt
     8521 gcactcccca ccccctccac ccatccctcg gagtgtgctc catgagattc cgctccccgc
     8581 gcagagccgc gcaggctctc gccgccctcg gcgccgcact cgccctcgcg gccggcggcc
     8641 cggccgcatc cgcccgggcc gccgccccgc cgctgccgga cctgaacatc accggcacct
     8701 acgtcaccgg cgtctcctcc ggcggcttca tggccaccca gctccaggtc gcctactccg
     8761 gcaccttcga cggcgccggc atcgtcgccg ccgggcctta cgactgcggc caggggcatg
     8821 tgatcgagtt cgtcacctgt gacctcggcc tgggcgtcgg cgacctggag cggcaggccg
     8881 aggactgggc gtccgagggc cgcatcgacc cggtcgcgaa cctgcggggc aaaccggtct
     8941 acgcgtacca cggcactctc gacccggtgg tcaacccatt ggtctcccgg gccggcgtcg
     9001 acttctacga gcacttcggc gcggacgtca cctaccacga ccgggacccg gccggacact
     9061 cgtggcccac tcctgacggc gtcgtcccct gcgccctcac tctgccgccg ttcctgaaca
     9121 actgtcacga cgacccggag caggagatgc tcactgcctg gctcggcggg gtcaacccgc
     9181 cgaacaccgg cgctcccagg ggcaccctca cgtacttcga ccagaacgcc tacgcgcccg
     9241 gcggctctgc ccgcgccctg tccatggact ccaccgggca gctctatctc ccgccggcct
     9301 gcgcgaacgg cgcgccctgc aaactggtgg tggccctgca cggctgtctg tccgcccagc
     9361 aactcctggg cacggccttc gccgagaaga gcaacatcga cacctacgcc gacaccaaca
     9421 acctggtcgt gctctacccg caggccgtct ccagcgtctg gcccgtcaat ccgcagggct
     9481 gctgggactg gttcggatac accggctccg actacgccgt gaagagcgca ccgcagatga
     9541 ccgccgtcgt caacatggtc cacgccctgg gcgcccgacc cgcaccggcc gtccccgcag
     9601 tgcccgttcc cgcagtgccc gccgtccgcg gcccgggcac tgcggggccg gggcctttgc
     9661 gccgacggga gcggaggtcc ggcggtccgc tcacggacgt gcggggacca gcgccgccgc
     9721 cggcgcacgc agggccgtcg tggtccggtc gcggtccccg gtggtgacca ggtccaggag
     9781 cggtcgggaa cgccgggatc gccggggccg gcggccccga agaggtgctg ggcgtcgggg
     9841 agcatggact gggcgtgacg caggacagtg ggcccctggc cgcggccgtg cggcgtcgac
     9901 cgggagcgtg tctgcccccg ccaccctcgc gcgcgccgcc ttccgcgggc cccggaggtg
     9961 tggcgcaggg ccaccagcgg cggcgcaccc gccggcctcc tcctctacaa cctcctccac
    10021 aacgccgatc cgacgtcgcg caccgatgtc tcgcaccgat gtcgcgcacc gatgtcgcgc
    10081 accgatgtcg cgctccgacg tcccgcaccg atgtcgcgcg ctgatgtcgc gctccgacgt
    10141 cccgcaccga cgtcgcgctc cgatgtcgcg ctccgatccc tcggacggcc tgaagccggt
    10201 cgtcacgcgc gggtggctct acacccgtgc cccacgcgcc gccgtcctcg gcgctccccg
    10261 cgtcgccgtc ctcgacgctc cccgtgcggc cgccctcggc gtccgaccgg cgccgactgc
    10321 gcagaccgct gccggcgctg ctggggcgca tcggtttcgg ggcgcgtcct gaggacgacc
    10381 catttcccct gccggccctc gccccgggcg cccgtcctgt cgaggcctgt gccggacgcc
    10441 tcctggaagc ggctacgtga acagccccca gcgatggata tgtgctgcct cagccatcag
    10501 acacgccttg gcgcaacgcc ggatggtcgg cgacgaccgt gcagctgccc ggtgcgatct
    10561 ccgtgaagcc ggcatcccgc acgagcggca gcccgctgcc ggtcagccgg gcccagtcgg
    10621 cggggtccgc cgtgcggacg cagagcgcga agcccgtgtc ccgccaggcc gtgcgctccg
    10681 ccgcgggcag tgcccaccag gccagctggg cgccgtgccc cgcctgggcc atggtctttc
    10741 cggccgacat ctccagagca ggattcagcc acagcaccgg ctgcgccgac tccgccgcga
    10801 cgggcggctc cgggtcgtcc agctcggtgc cggacacctg gagcttggcc aggtccttgg
    10861 gccagccgtc cagcgggacc ggcgggaaga cccgcacctg cgcggccttc ccggtcaccg
    10921 tgatcccggg cagcgcctcg gcccgccgcc actcggcgcc ccgggcccgc cgtacgacct
    10981 tgcggatccg cgcgtcctcc cagttccgca ccgcctcggc ccactcgccg tcaccggccg
    11041 cccgctcgtc cgcgagcagc acgagcacgg cccgggcggc cgtctccagc gcgtcggtgc
    11101 gggcgggcgg cgcagcgcgc tcgatccgca cgacgagggg aaggacgaac tgcggcgctt
    11161 cgtcccggct gtcactcggg ggcatggcat cagggctcac gtcagccaac cttaaagggc
    11221 gcaaccggcc gagaacgtct tgcggaccgg acccctgatc agctccgacg gcaagagcgg
    11281 acaaaatcgc cccatgcaac gtgacctacg actgatcggc gta
//
