LOCUS       NZ_CP047020            10486 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces broussonetiae strain T44 chromosome.
ACCESSION   NZ_CP047020
VERSION     NZ_CP047020.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces broussonetiae
  ORGANISM  Streptomyces broussonetiae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9735779)
  AUTHORS   Mo,P.
  TITLE     Streptomyces sp. strain T44 isolated from rhizosphere soil of
            Broussonetia papyrifera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9735779)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) College of Life Science and Technology,
            Central South University of Forestry and Technology, No. 498,
            Shaoshan South Road, Changsha, Hunan 410004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2019
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009796285.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:56:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,022
            CDSs (total)                      :: 8,931
            Genes (coding)                    :: 8,515
            CDSs (with protein)               :: 8,515
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 416
            CDSs (without protein)            :: 416
            Pseudo Genes (ambiguous residues) :: 0 of 416
            Pseudo Genes (frameshifted)       :: 171 of 416
            Pseudo Genes (incomplete)         :: 334 of 416
            Pseudo Genes (internal stop)      :: 29 of 416
            Pseudo Genes (multiple problems)  :: 110 of 416
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:31:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3690130
            Orig. end                         :: 3700616
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047020.1.
            Bacteria and source DNA available from Zhao YL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10486
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="13"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10486
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10486
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5486](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5486)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(364..1974)
                     /locus_tag="GQF42_RS17115"
                     /old_locus_tag="GQF42_17115"
     CDS             complement(364..1974)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435092.1"
                     /locus_tag="GQF42_RS17115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17115"
                     /product="dynamin family protein"
                     /protein_id="WP_158920875.1"
                     /transl_table=11
                     /translation="MVTLDVRPQLLDTLSALRDRVAAARFPLPLAGAPRARANRDELLA
                     QLDDYLVPRLRDPEAPLLAVVGGSTGAGKSTLVNSLVGRRVSEASVLRPTTRTPVLVCH
                     PEDHHWFSDMRVLPDLTRVWSPQHETTDDLLMSGAEHPARVLRVESAETLPPGLALLDA
                     PDVDSLIADNRALAAELICAADIWIMVTTAARYADAVPWHLLRTAKEYDATLVTVLDRV
                     PHQVVSEVSRQYGALLTKAGLGDVPRFTVPELPESAWGAGLLPTTAVAPLKDWLAHHAQ
                     DPAARRHVMLRTAHGLLDSLRSRMSELAGAAAAQYAAALRLTTAVEAAYDGEHARVRDR
                     LRSGAVLSGDALKRWRAFPLDCTAGELLDALVESLVALLLCAVTAADERVDEAWRLEPA
                     GADPALAAGESSVESAEHRIGLAVRRWRRELEEYAEEESRDLERSGAPDPEAVAALVAT
                     ALLGGRRARTAGEGLAERLGAHGALRLRDRAGRLLGERVDRVMLRERERRLAPLDALDV
                     HPEPQAELIAALSLLQKER"
     misc_feature    1974..1992
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.4"
                     /tool="antismash"
     misc_feature    complement(1985..2001)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.58"
                     /tool="antismash"
     misc_feature    complement(1990..2004)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.96"
                     /tool="antismash"
     tRNA            2248..2320
                     /anticodon=(pos:3692411..3692413,aa:Arg,seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="GQF42_RS17120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="GQF42_17120"
                     /product="tRNA-Arg"
     gene            2248..2320
                     /locus_tag="GQF42_RS17120"
                     /old_locus_tag="GQF42_17120"
     gene            2628..3587
                     /locus_tag="GQF42_RS17125"
                     /old_locus_tag="GQF42_17125"
     CDS             2628..3587
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672797.1"
                     /locus_tag="GQF42_RS17125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17125"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_158920877.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1.1e-53, bitscore:
                     180.3, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQGVATTLADWSVIEDEVEAVALREHLDTEDDLAA
                     ALADFDIVVTLRERVPVPGSLIARLPRLKLIVASGMRNTVIDYAAAEKHGVTVCGTQSS
                     GIPPAELTWALLLGLARGIVEENTALRTGGPWQSTVGADLHGARLGVLGLGRIGGHVAR
                     VGLAFGMRVSAWSPRLTEERAAEHGVELAASKEELLTASDFVSVQVPGGDGTRGLIGAA
                     ELALMKPTAYLVNTSRASVVDQDALLAALHEGRIAGAGIDVFDIEPLPADHPMRTAPRL
                     LATPHLGYVSRANYTTYYGQAVENIQAYLAGSPIRRLP"
     gene            3730..4023
                     /locus_tag="GQF42_RS17130"
                     /old_locus_tag="GQF42_17130"
     CDS             3730..4023
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672799.1"
                     /locus_tag="GQF42_RS17130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17130"
                     /product="chaplin"
                     /protein_id="WP_158920879.1"
                     /transl_table=11
                     /translation="MSRIAKAAVVALGTGAVVVGGAGLALADAGAEGAAVDSPGVLSGN
                     LLQVPVNVPVDVCGNTVDAIALLNPAFGNTCVNKGDLKPGNPGGYGNGGYGH"
     gene            complement(4151..5017)
                     /locus_tag="GQF42_RS17135"
                     /old_locus_tag="GQF42_17135"
     CDS             complement(4151..5017)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="GQF42_RS17135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17135"
                     /product="tyrosinase family protein"
                     /protein_id="WP_158920881.1"
                     /transl_table=11
                     /translation="MAYVRRDVGTLTASERRRFVNALLELKRRGEYDEFVRTHIAHYTP
                     DGETGLRTAHMAPSFLPWHRRFVLELERALRRVDSSVTVPYWDWTRDRTPASVPWTKDL
                     LGGNGRRFDQQVTTGPFAYAAGHWTIKEGVTDGRFLTRDLGRAAAPLQLPTKSDLRWAL
                     DDPVYDTPPWDSTVTSGFRNKLEGWGQGSGSASWHNHNRVHRWVGGVMLGGASVNDPVF
                     WLHHAFVDLQWQRWQRAHREHRYLPADPPGPADDQNGRVVARYGKLPPWDETPDQLEDL
                     SRIYRYA"
     gene            complement(5001..5486)
                     /locus_tag="GQF42_RS17140"
                     /old_locus_tag="GQF42_17140"
     CDS             complement(5001..5486)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="GQF42_RS17140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17140"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_158920883.1"
                     /sec_met_domain="melC (E-value: 1e-43, bitscore: 147.9,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVFGIDGATVGAGRQGKEGAARAARRDVARGLLASVAALALAPVV
                     GASRPVPPMRSSDGTSFDEVYRGRLIQGVLVPVVEGGTTEEDWRITVDGRPLHLMRRAD
                     GTWLSMVDHYTSYRTPLEATRAAVDEIGPGQQLRDDLATDPMGGEQMHMGEDRGVRA"
     misc_feature    complement(5551..5564)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.92"
                     /tool="antismash"
     gene            complement(5838..6359)
                     /locus_tag="GQF42_RS17145"
                     /old_locus_tag="GQF42_17145"
     CDS             complement(5838..6359)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672802.1"
                     /locus_tag="GQF42_RS17145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17145"
                     /product="hypothetical protein"
                     /protein_id="WP_158920885.1"
                     /transl_table=11
                     /translation="MKSTTRGTLAAVVTGVAAAVGAAATPAAAVGTVPISVPLEGASES
                     LGMEMPKAGLELPAPLMMPGAPQGPRYVTGHLLPDRTLPPVPMSGGLPGVNLDAPIPRV
                     LGDRFDHVAVDTPVNDLHTLTPGVVADAPLTRPNPDNFGLPDLKEPQLGIIAPALQTIA
                     DADVAGGAGR"
     gene            complement(6476..7060)
                     /locus_tag="GQF42_RS17150"
                     /old_locus_tag="GQF42_17150"
     CDS             complement(6476..7060)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479492.1"
                     /locus_tag="GQF42_RS17150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17150"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_158920887.1"
                     /transl_table=11
                     /translation="MPKPLAGGRGFGLLLVLTGAAGLLASWAITLDKFKLLQNPNFVPG
                     CSLNPVVSCGSVMKSEQAAAFGFPNPMLGLVAYGIVVCVGMSLIAGARFPGWYWLLFEA
                     GCLFGVGFVSWLQYESLYRINALCLWCALAWVATILMFWYATSLVVAHGFLPAPTWLKG
                     FLAEFTWVLPVLHIGVVGMLILTRWWDFWTS"
     gene            complement(7060..7551)
                     /locus_tag="GQF42_RS17155"
                     /old_locus_tag="GQF42_17155"
     CDS             complement(7060..7551)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065676.1"
                     /locus_tag="GQF42_RS17155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17155"
                     /product="DUF5949 family protein"
                     /protein_id="WP_158920889.1"
                     /transl_table=11
                     /translation="MTSTPSDIRPAGLGTLVLLAWSGEAPDGADMPYLLAYSLGDAEGG
                     PEATAAAVEHLLTSNGLPVGGDLVDGNARPSLPVSLLVEAGQAVVRMPQLVAQAGAPPE
                     WLEAVAERGYAYLVFTTRAWPEGEPGKTVEPADLAAFAGAEETLNAAAHIVLPAARVRH
                     "
     gene            complement(7712..8002)
                     /locus_tag="GQF42_RS17160"
                     /old_locus_tag="GQF42_17160"
     CDS             complement(7712..8002)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136064.1"
                     /locus_tag="GQF42_RS17160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17160"
                     /product="hypothetical protein"
                     /protein_id="WP_158920891.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSVALAGVAAPAFAYNAADVTPTSLNGAVNQLTK
                     GPLTLNDAMPIHHQSDALNTENKNSLLHTVKGATGALNQHNPLLGGLPLQS"
     gene            complement(8190..8840)
                     /locus_tag="GQF42_RS17165"
                     /old_locus_tag="GQF42_17165"
     CDS             complement(8190..8840)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GQF42_RS17165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GQF42_17165"
                     /product="hypothetical protein"
                     /protein_id="WP_158920893.1"
                     /transl_table=11
                     /translation="MRVLSARRLASTTLCAAVLAGVTCPAAVAADAAREHASSASSPAT
                     QAEEQLLVQVRALDATRVVLQPVVDLLNESLKKGRLTSEEADRLGGAARRAINEVARTA
                     PVLPAPAAPRAPVTPGLSSTAKLPMVPDRPAVVKHADDGKAPAARDAVGDLLASLQTAV
                     NNLLKAATSGLSTVVSAAGGVVSNLVGLLTSTLGLATSNAASPASVPNLPATG"
     misc_feature    complement(8829..8844)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.63"
                     /tool="antismash"
     misc_feature    8917..8930
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.74"
                     /tool="antismash"
     gene            complement(9012..9251)
                     /locus_tag="GQF42_RS17170"
                     /old_locus_tag="GQF42_17170"
     CDS             complement(9012..9251)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="GQF42_RS17170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17170"
                     /product="chaplin"
                     /protein_id="WP_158920895.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSAAVVAVAGGAGVACADSGAAGVAKNSPGVLSGN
                     TVEIPIHVPIALCGNTVDVIGLLNPGFGNTCVNH"
     gene            9469..9840
                     /locus_tag="GQF42_RS17175"
                     /old_locus_tag="GQF42_17175"
     CDS             9469..9840
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672808.1"
                     /locus_tag="GQF42_RS17175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_17175"
                     /product="hypothetical protein"
                     /protein_id="WP_158920897.1"
                     /transl_table=11
                     /translation="MRKLLGPHTVRHTVSEDFTGAGDRRTLSSGPRRTPVTAIRRTSVA
                     FGGSVNPPDDVPAPTTSAPGPTPARRISGYANTLGHDSDLSDLGPDRRRGGDQPGFRLV
                     PLPETAWADVLFAAVDAQV"
ORIGIN
        1 gtcccctgcg tcccctgcgt cccctggacc tgtttcacct gctccaccca ctccaccggc
       61 tctgtcttgc cggtacgcac ccatcgtgcc gacgccgccg tgcccggcag cgccccggcg
      121 ggcgccgccc tcgtcctgca cgcgcgcgta cggctggctc accgcgccgt cgtcgccggc
      181 cccggccgcg ccgtccgtgc cgtcggcccg gatcccggtg ccggtgccgg acgtaccggt
      241 acggccgtcc atcgcgccct cgcccagctc cacccggtcg ttgctgtcgt gctccatgcg
      301 ccccacgcgt cccaagtggt caatgtgatc cgtgtgctcc tggtcagtga cagcggtcac
      361 cggtcacctc tccttctgca gcagcgacag tgcggcgatg agttcggcct ggggctcggg
      421 atggacgtcc agggcgtcga gcggggcgag gcgccgctcg cgctcgcgga gcatgacgcg
      481 gtccacgcgc tcgcccagca ggcgtccggc ccggtcgcgc agccgtagcg cgccatgggc
      541 gccgagccgc tcggcgagcc cctcgcccgc ggtgcgcgcc cggcgtccgc cgagcagcgc
      601 ggtggcgacc agggcggcga cggcctccgg atcgggggcg ccggagcgct ccagatcgcg
      661 cgattcctcc tcggcgtact cctccagttc gcgccgccag cgccgtacgg cgaggccgat
      721 gcggtgctcg gcgctctcca cggaggactc gccggcggcg agggcggggt cggctccggc
      781 gggctcgagc cgccaggcct cgtcgacgcg ctcgtcggcg gcggtgacgg cgcacagcag
      841 cagggcgacc aggctctcca cgagcgcgtc caggagttcc ccggcggtgc agtccagcgg
      901 gaaggcccgc cagcgcttga gggcgtcccc ggagagtacg gcgccggacc gcagccggtc
      961 gcgcacgcgc gcgtgctcgc cgtcgtaggc ggcctccacg gcggtggtga gccggagcgc
     1021 ggcggcgtac tgggcggcgg ccgcgccggc cagctcggac atccgggacc tgagcgagtc
     1081 gagcaggccg tgggcggtac ggagcatgac gtgccgacgc gcggcagggt cctgggcgtg
     1141 atgggcgagc cagtccttca gcggggcgac ggcggtcgtg ggcagcagcc cggcgcccca
     1201 ggcggactcg ggcagctcgg gcacggtgaa gcggggtacg tcgccgagcc cggccttggt
     1261 gagcagggca ccgtactgcc gggagacctc ggagaccacc tggtggggca cccggtccag
     1321 gacggtcacg agcgtggcgt cgtactcctt ggcggtgcgc agcaggtgcc agggtacggc
     1381 gtcggcgtag cgggcggccg tggtgaccat gatccagatg tctgcggcgc agatcaactc
     1441 ggcggccagg gcgcggttgt cggcgatcag ggagtcgacg tccggtgcgt cgaggagggc
     1501 gagtccgggc ggaagggtct cggcgctctc gacgcgcagc acgcgcgcgg ggtgctcggc
     1561 gccggacatc aggaggtcgt cggtggtctc gtgctggggc gaccacacgc gcgtgaggtc
     1621 gggcaggacc cgcatgtcgc tgaaccagtg atgatcctcc ggatggcaga caagcaccgg
     1681 cgtccgggtc gtcggccgca gcacgctcgc ctcgctcacc cgtcggccga cgagcgagtt
     1741 gacgagcgtc gacttgccgg cgccggtgga accgccgacg acggccagca agggcgcctc
     1801 ggggtcccgc agacggggta ccagatagtc gtcgagctga gcgagcagtt cgtcgcggtt
     1861 ggcacgcgcg cgtggggctc cggccagggg cagcggaaag cgtgcggcgg cgacacggtc
     1921 gcgcagcgcg gagagtgtgt cgagcagctg aggccgtacg tccaaggtca ccacatgcga
     1981 agaatgccca attttagggg aattctgaag catatagaca tgtctgcgcg ccgacggcgc
     2041 acaaagggca gaagggatga ctgggacgcg cgcgcagtcc aggcataacg agtgcacaac
     2101 acccggtgcg tcggacgcca aaagcgatgc acgattcgta cctgcctgcg attatcagga
     2161 ccgcttcacc gaacctccac atcgagccac ggaggcgaag cgacagggtc gaggaacagg
     2221 gagctttatc cttggtcccg ccaccaggcc cccgtagctc agtggataga gcaggcgcct
     2281 tctaagcgct tggccgcagg ttcgagtcct gccgggggcg cccgtgcaca gccctccctc
     2341 agggagggct ttttttgctg gtcagaggtg gtaccacggg gcgcgggtca gcccgggacg
     2401 cctccttggt ggccaagggt gcgcttcggg accgtccggc tgtgaccggg gtgtcgcggg
     2461 tggccctgcc gcagatcatg tcgaccttct cgaccagagg aagatcagcc aacgcccggc
     2521 acctgggcct ctgggggtcg ggcgcgggtg gtccggggcg ccgttcccct ctcccggccc
     2581 gtcggcgtgg cgcggataca gtcatcctcg gggagaggag agtcgacgtg cgacttcgct
     2641 gtgcggtact ggacgacttc cagggcgtgg ccaccacgct ggccgactgg tcggtgatcg
     2701 aggacgaggt cgaggccgtc gcactgcgcg agcacctgga caccgaggac gacctcgccg
     2761 cggccctcgc cgacttcgac atcgtcgtca ccctgcgcga acgcgtcccg gtccccggct
     2821 cgttgatcgc ccggctgccc cggctgaagc tgatcgtcgc ctccgggatg cgcaacaccg
     2881 tcatcgacta cgcggccgcc gagaagcacg gcgtcaccgt gtgcggcacg cagagttcgg
     2941 gcatcccgcc cgccgagctg acctgggcgc tgctgctcgg gctggcccgc gggatcgtcg
     3001 aggagaacac cgccctgcgc accggcggtc cctggcagtc caccgtcggc gccgacctgc
     3061 acggcgcccg gctcggcgtg ctcggtctcg gccggatcgg cggccatgtg gcccgggtcg
     3121 ggctcgcctt cggcatgcgg gtcagcgcct ggagcccgcg cctgacggag gagcgcgccg
     3181 cggaacacgg tgtcgaactg gccgcgtcca aggaggaatt gctcaccgcc agtgacttcg
     3241 tctccgtcca ggtgcccggc ggtgacggca cccgcggcct catcggcgcc gccgaactgg
     3301 ctctcatgaa gccgaccgcc tacctggtca acacctcccg ggcctcggtc gtcgaccagg
     3361 acgcgctgct ggccgcgctg cacgagggcc ggatcgcggg agcgggcatc gacgtcttcg
     3421 acatcgagcc gctgcccgcc gaccatccga tgcgcaccgc gccgcgcctg ctcgccacgc
     3481 cccacctcgg ctatgtctca cgggccaact acacgacgta ctacggccag gcggtggaga
     3541 acatccaggc ctatctggcg ggctccccga tacgacggct gccgtgagtg ccgtcgacga
     3601 aaaggcatgc tgccgttgaa agggtgatcc acctgcgatc accgtgacca ccctcgcgcg
     3661 gcgccgttga acggaaagtg cggcggctgg cctgccgctg cgctttccga gcaacaaaag
     3721 gagaacgtga tgtctcgcat cgcgaaggca gccgttgtcg ccctcggcac cggtgccgtg
     3781 gtggtcggcg gggccgggct ggccctggcc gacgcgggcg ccgagggcgc ggccgtcgac
     3841 tcgccgggtg tgctgtccgg caacctgctc caggttccgg tcaacgtccc ggtcgacgtg
     3901 tgcgggaaca ccgtcgatgc gatcgccctg ctgaaccccg ccttcggcaa cacctgcgtc
     3961 aacaagggcg acctgaagcc gggcaacccc ggcggctacg gcaacggcgg ctacggccac
     4021 tgaccggacc ggccgacgcg acgatccggc cgtcagcggc agaccccaag agccccggca
     4081 ctgcgagcgc cggggctctt cccctgcccc tgcccggcag cgcccgtctc gtgcccggga
     4141 gcccgcggcc tcaggcgtac cggtagatcc ggctcaggtc ctccagctgg tccggtgtct
     4201 cgtcccacgg cggcagtttc ccgtagcggg cgacgacccg cccgttctgg tcgtcggcgg
     4261 ggccgggcgg gtcggccggc agatagcggt gctcgcggtg cgcccgctgc cagcgctgcc
     4321 actgcaggtc gacgaaggcg tggtgcagcc agaacaccgg gtcgttgacc gaggcaccgc
     4381 cgagcatcac cccgccgacc cagcggtgca cccggttgtg gttgtgccag gaggcgctgc
     4441 cgctgccctg cccccagccc tccagcttgt tgcggaagcc gctggtcacg gtcgagtccc
     4501 acggcggagt gtcgtagacg gggtcgtcga gcgcccaccg caggtcgctc ttcgtgggca
     4561 gctgcaaggg ggcggcggca cggcccaggt cccgggtgag gaacctgccg tcggtgacgc
     4621 cctccttgat ggtccagtga ccggccgcat aggcgaacgg cccggtcgtc acctgctggt
     4681 caaagcgccg tccgttgccg ccgagcaggt ccttcgtcca cggcaccgag gccggcgtac
     4741 ggtcccgggt ccagtcccag tacggcaccg tcaccgagga gtcgacgcgg cgcagcgccc
     4801 gctccagctc cagcacgaac cggcggtgcc agggcaggaa ggacggcgcc atgtgcgcgg
     4861 tgcgcagacc ggtctcgccg tcgggggtgt agtgggcgat gtgcgtgcgg acgaactcgt
     4921 cgtactcacc gcgccgcttg agttccagca gggcgttcac gaaccggcgc cgctcgctcg
     4981 cggtgagcgt gccgacgtcc ctacgcacgt acgccacggt cttcccccat gtgcatctgt
     5041 tcgccgccca tcgggtccgt ggccaggtcg tcgcgcagct gctggccggg gccgatctcg
     5101 tccacggccg cccgggtcgc ctccagcggt gtccggtacg aggtgtagtg gtcgaccatg
     5161 ctcagccagg tgccgtcggc ccgccgcatc aggtgcagcg ggcgcccgtc gacggtgatc
     5221 cgccagtcct cctcggtcgt cccgccctcg acgaccggca ccagcacgcc ctggatgagg
     5281 cggccccggt agacctcgtc gaaggaggtg ccgtccgagg accgcatcgg gggcacgggc
     5341 cgggacgcgc cgacgaccgg cgccagtgcc agcgcggcga ccgaggccag cagcccccgc
     5401 gccacgtccc tccgggccgc tctcgccgcc ccctctttcc cctgccgccc cgcgcccacc
     5461 gtcgctccgt cgataccgaa gaccatcacg cgctcctcgc ctggaaccgg acgcccacga
     5521 ccggacgccg taccggctaa ccggcccggg gcacggaggg tcaccgcacc agagccatcc
     5581 ggtggggagg agcggctacg acgcccggct cgaacggggg cgcggtggag gcggtgcggc
     5641 ggcttcggcg gcttcggcgg tcgagtacaa cggaccggag aacgggaccg gagaacggga
     5701 ccgggcaaca ggaccgggcg gcggagacgg ccgggccgac aaggggcgca gcccttcgta
     5761 ccccagcgcg cgccccgcac ggctcacggc tcacgggcca cgggccacgg gcccacggcc
     5821 cacagccccc tgagggccta ccggcccgcg ccgcccgcca cgtccgcgtc ggcgatcgtc
     5881 tgcagggccg gggcgatgat gccgagctgc ggctccttga ggtccgggag gccgaagttg
     5941 tccgggttgg gccgggtcag gggggcgtca gcgaccacgc cgggggtcag cgtgtgcagg
     6001 tcgttgacgg gggtgtcgac ggccacgtgg tcgaagcggt cgccgaggac ccgcgggatc
     6061 ggtgcgtcga ggttgactcc gggcaggccg ccggacatcg gcaccggggg cagggtgcgg
     6121 tcgggcagca ggtggccggt gacgtagcgc gggccttggg gtgcgcccgg catcatcagc
     6181 ggcgccggca gctccaggcc cgccttcggc atctccatgc cgagggactc ggatgcgcct
     6241 tccaggggca cggaaatggg gaccgtgccg accgcggcgg cgggggtggc tgcggcaccc
     6301 acggcggccg cgacgcccgt gacgacggcg gcaagggttc ctcgggtggt cgacttcatg
     6361 tcaggtacgg tcccttccag gatgcgggag ttcgacgtcc gtaccgcgta acgagccgga
     6421 aaaccacttc tgttcacaat tcgcccggct gccgtaattg tcggctggtc actcttcagc
     6481 tggtccagaa gtcccaccag cgggtcagga tcagcatgcc gaccacgccg atgtgcagca
     6541 ccgggagcac ccaggtgaac tcggcgagga accccttcag ccaggtcggc gcgggcagga
     6601 agccgtgcgc gacgaccagc gaggtggcgt accagaacat gaggatcgtg gcgacccagg
     6661 ccagagcgca ccacaggcac agcgcgttga tccggtacag ggactcgtac tggagccagg
     6721 agacgaatcc gacgccgaac aggcagccgg cctcgaacag cagccagtac cagccgggaa
     6781 agcgggcccc ggcgatcagg ctcataccga cgcacacgac gatgccgtag gccaccaggc
     6841 ccagcatcgg gttggggaac ccgaaggccg cggcctgctc gctcttcatc acactgccgc
     6901 aggacaccac agggttcagg ctgcatccgg gcacgaagtt cgggttctgc agcagcttga
     6961 acttgtcgag cgtgatggcc caggaggcga gcagcccggc cgccccggtg agtaccagca
     7021 gcagcccgaa tcccctgccg ccggccaggg gcttgggcat cagtggcgca ctctggcggc
     7081 cggcaggacg atgtgcgcgg cggcgttcag ggtctcctcg gcaccggcga aggcggcgag
     7141 gtcggccggc tcgaccgtct tgccgggctc gccctcgggc caggcgcggg tcgtgaacac
     7201 gaggtaggcg tagccgcgct cggcgacggc ctcgagccac tccggcggcg caccggcctg
     7261 cgcgaccagc tgtggcatcc gtacgacggc ctggcctgcc tcgaccagca ggctgacggg
     7321 gaggctgggc cgggcgttgc cgtcgaccag gtcgccgccg accggcagcc cgttgctggt
     7381 cagcaggtgc tcgaccgccg ccgcggtggc ctcggggccg ccctcggcgt cacccaggga
     7441 gtaggccagc agatagggca tgtcggcgcc gtcgggggcc tcgccgctcc atgcgagcag
     7501 gacgagcgtg ccgaggccgg ccgggcggat gtcgcttggg gttgaggtca ccgcgcgacc
     7561 ctatcgacgc ttccgcgggc cctctgacgg cttctcaccc ttccggacga gcgcccggac
     7621 ccgctccaca cgaacgaggg aagcggggcc gcaaaagccg ggggccggtg ccgcgggtgc
     7681 ggtaccggcc tccgtgcgta cgggcggggt gtcagctctg caggggcagg ccgccgagca
     7741 gcgggttgtg ctggttcagc gcgccggttg cgcccttgac cgtgtggagc agggagttct
     7801 tgttctcggt gttgagcgcg tccgactggt gatggatcgg catcgcgtcg ttgagggtga
     7861 gcgggccctt ggtgagctgg ttgacggcgc cgttgaggct ggtgggcgtc acgtcggcgg
     7921 cgttgtaggc gaacgcgggc gcggcaacgc ctgcgagggc cacggaaccg gcaacgacag
     7981 cggcagcctt cagggacttc atcgtgttcc ttccttcggc aactcaagtc accccagtga
     8041 attctgacgc cgtgcctaac gagatccggc cggggcggaa accccgtacg cacaagacat
     8101 atgagatccc cgaggttcgg ctcctcggga acagcactca gccgccgtac cgggcggtac
     8161 ggcggctgag tggtgcacgc gccgaagggt cagccggtcg cgggcaggtt cggtaccgag
     8221 gccggggagg ccgcgttcga ggtggccagg ccgagcgtgg acgtcagcag gccgaccagg
     8281 ttggacacca cgccgccggc cgccgagacc accgtggaca ggccggaggt ggccgccttg
     8341 agcaggttgt tgaccgcggt ctggagagag gcgagcaggt cgccgacggc gtcgcgggcc
     8401 gcgggggcct tgccgtcgtc ggcgtgcttc acgacggccg ggcggtccgg gaccatcggg
     8461 agcttggcgg tcgaggagag gccgggcgtc acgggagccc tgggcgcggc cggtgccggc
     8521 aggaccgggg cagtcctggc cacctcgttg atggcccgtc gggccgcccc gccgagcctg
     8581 tcggcctcct cggaggtcag tctgcccttc ttcagcgact cgttgagcag gtcgacgacc
     8641 ggctggagta cgacacgggt cgcgtccagg gctctgacct ggacgagcag ttgctcctct
     8701 gcctgcgtgg cgggagagga ggcgctggac gcgtgttctc gggccgcgtc ggcggcaacg
     8761 gctgccgggc aggtgacacc ggccaggacg gcggcgcaga gggtggtgga cgcaaggcgc
     8821 cgcgcggaca gaacacgcat gggagttcct ttcggtgaac agcgggacct gtgcccaccg
     8881 tccgttcacc gacagcgcct cgcaaccggt ggatcatgga tctgatccat ccgggggagc
     8941 ggaggaatcg gccgtccgcc ccgtggcgga tacgcgggac ggacggccgg ccgaagggtt
     9001 gtgtcccggc gtcagtgatt gacgcaggtg tttccgaagc ccgggttcag cagaccgatg
     9061 acgtcgacgg tgttgccgca gagggcgatc ggcacgtgga tcgggatctc gacggtgttg
     9121 cccgacagaa cgcccggcga gttcttggcg acgccggccg caccggagtc ggcgcaggcc
     9181 acaccggcac cgccggcgac ggccacgacg gcggcggagg tcaggaccag acccttcgcg
     9241 atacgcgaca tggagaggtg ctccttgggg atcgaggcaa acatgcgggc ggacgcccgg
     9301 cgttgtgtca acgcgggccg gcgccggtag ttgtgccgcc aacggggtga tacacgcaaa
     9361 gggttaccct tcacacttcc gacacacttg tcctctttca acggattaat acgcatagcg
     9421 gctagagtta cggccctctc cagcacatgc gaaagggcac cgccggtcat gcgcaagctc
     9481 ctgggtccgc acacggtgcg gcacacggtc agcgaggact tcaccggcgc gggcgaccgg
     9541 cgcacgctct cctccggccc ccggcgtacg cccgtcacag cgatccggcg tacgtctgtc
     9601 gcattcggcg gctccgtcaa tccacctgac gacgtacccg cccccacgac cagcgcaccg
     9661 gggcccacac ccgcccggcg gatctctggc tacgccaaca cgctcggcca cgactccgac
     9721 ctgtccgatc tcggcccgga tcggcggcgc ggcggtgacc agccgggctt ccggctcgtt
     9781 cccctgccgg aaacggcatg ggccgacgtc ctcttcgccg ccgtggacgc acaggtgtga
     9841 ggcgcgttcc cgaccgccgt cccgagcgcc ccccctgtga acgaggtatt ccgcccatgc
     9901 accagccagc gcccgccccc cggccacggg tcctgcacct cgcgcaaccc gtcgacggcg
     9961 gtgtcgcccg ggtcgtcctg gatctggcgc gcgcccagct cgcggcgggc ctgcacgtca
    10021 cgacggcctg tcccgacggt gaactcgcct tacagttgcg ggagttgggc gtcgacgtcc
    10081 accactggcg ggccggccgc gccccgggcc cgggcctgcc gggcgaggta cggcggctcg
    10141 tacgactgct cgacgaggta cggcccgaac tggtgcacgc gcacagtgcc aaggcgggcc
    10201 tggccggccg gctcgccgtc cgcggccgga tccccaccgt gttccagccg cacgcctggt
    10261 ccttcgaggc ggtcggcggc gccacggcgg cgctcgcgct gcgctgggag cggtgggggg
    10321 cgcgctgggc cgaccgggtc gtgtgcgtca gcgaggcaga gcgcaccacc gggctgcgcg
    10381 ccggggtccg ggccgcgtac gccgtcgtcc cgaacggcat cgacaccgca cgcttctcac
    10441 cgggcccgca cgcccccctc ggatccgctc ctctcgtggt ctgcgt
//
