LOCUS       NZ_CP047020            10405 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces broussonetiae strain T44 chromosome.
ACCESSION   NZ_CP047020
VERSION     NZ_CP047020.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces broussonetiae
  ORGANISM  Streptomyces broussonetiae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9735779)
  AUTHORS   Mo,P.
  TITLE     Streptomyces sp. strain T44 isolated from rhizosphere soil of
            Broussonetia papyrifera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9735779)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) College of Life Science and Technology,
            Central South University of Forestry and Technology, No. 498,
            Shaoshan South Road, Changsha, Hunan 410004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2019
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009796285.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:56:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,022
            CDSs (total)                      :: 8,931
            Genes (coding)                    :: 8,515
            CDSs (with protein)               :: 8,515
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 416
            CDSs (without protein)            :: 416
            Pseudo Genes (ambiguous residues) :: 0 of 416
            Pseudo Genes (frameshifted)       :: 171 of 416
            Pseudo Genes (incomplete)         :: 334 of 416
            Pseudo Genes (internal stop)      :: 29 of 416
            Pseudo Genes (multiple problems)  :: 110 of 416
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:31:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2634172
            Orig. end                         :: 2644577
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047020.1.
            Bacteria and source DNA available from Zhao YL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="12"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    182..191
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(1101..1114)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    1498..1512
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     misc_feature    1843..1857
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            2443..2898
                     /locus_tag="GQF42_RS12170"
                     /old_locus_tag="GQF42_12170"
     CDS             2443..2898
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140784.1"
                     /locus_tag="GQF42_RS12170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_12170"
                     /product="DinB family protein"
                     /protein_id="WP_158919652.1"
                     /transl_table=11
                     /translation="MLESRLDFHRATLELKCRGLDDAQVRLAPAEPSSPALLGLVQHLA
                     EVERNWFQRVPAGLEVPPPFADETGYGLDPERGLQEALDVWRAQIARGRELLAGREPDD
                     TGRMADKPPAGVEVGVRRVLIHLVEEYARHNGHADILRERIGETTGA"
     misc_feature    2900..2915
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.6"
                     /tool="antismash"
     misc_feature    complement(2905..2918)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.62"
                     /tool="antismash"
     misc_feature    3031..3046
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3165..3683
                     /gene="ectA"
                     /locus_tag="GQF42_RS12175"
                     /old_locus_tag="GQF42_12175"
     CDS             3165..3683
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671932.1"
                     /locus_tag="GQF42_RS12175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_12175"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_158919653.1"
                     /transl_table=11
                     /translation="MTAAPADLLIDHPAVNDGATLWRLAKDSKTLDLNSSYSYLLWCRD
                     FAGTSAVARGADGEPVGFVTGYVRPDRPHTLLVWQVAVDSAHRGLGIAAALLDGLTARL
                     VAERGVTEVETTITPGNTASERLFTSFAARHGAHLEREVLFPAELFPDGSHDPEVLYRI
                     GPLTEFTAH"
     gene            3697..4968
                     /gene="ectB"
                     /locus_tag="GQF42_RS12180"
                     /old_locus_tag="GQF42_12180"
     CDS             3697..4968
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 3.7e-73.
                     Score: 238.4. Matches aSDomain:
                     nrpspksdomains_GQF42_RS12180_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="GQF42_RS12180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_12180"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_158919654.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.3e-77, bitscore:
                     258.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMYDEDGHAY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLDRDGVTHGLDMSTTAKRSFLQAFQDLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALKELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGIAPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTATAALETYWTDGSAMEKQTRARGEQVEQAL
                     ISITEENLADVKEYRGRGLVWGLEFHAKDRASRIARRAFELGLLIETSGPQGEVVKLLP
                     ALTITPDELDEGLRTLARAVRETV"
     aSDomain        3781..4794
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GQF42_RS12180_Aminotran_3.1"
                     /evalue="3.70E-73"
                     /label="GQF42_RS12180_Aminotran_3.1"
                     /locus_tag="GQF42_RS12180"
                     /protein_end="366"
                     /protein_start="28"
                     /score="238.4"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHAYLDFFAGAGSLNYGHNNPVLKRALIDYLD
                     RDGVTHGLDMSTTAKRSFLQAFQDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALKELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIAPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TATAALETYWTDGSAMEKQTRARGEQVEQALISITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5405
                     /locus_tag="GQF42_RS12185"
                     /old_locus_tag="GQF42_12185"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="GQF42_RS12185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_12185"
                     /product="ectoine synthase"
                     /protein_id="WP_158919655.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.3e-52, bitscore:
                     175.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAESGTWESKRIVLARERVGFSLHETILY
                     AGTETSMWYANHVEAVVCTKGAAELTDHETGRAYTITPGTMYLLDGHERHTLRVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5412..6314
                     /gene="thpD"
                     /locus_tag="GQF42_RS12190"
                     /old_locus_tag="GQF42_12190"
     CDS             5412..6314
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="GQF42_RS12190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_12190"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_158919656.1"
                     /sec_met_domain="PhyH (E-value: 9.2e-41, bitscore: 139.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPTGFVTDLYPSRGTTEVGIPRQDPVVWGSPDTPGPIPTGDL
                     QSYERDGFLAIDQLITPDEVEVYRQELERLIADPAIRADERSIVEPASKEIRSVFEVHR
                     ISKVFANLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGL
                     PNMRTVSVSIALTENHDTNGGLMIMPGSHRTFLACAGATPKDNYKKSLQMQDAGTPSDE
                     ALTKMAGAYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENAAVEPF
                     AAPVRRPEFIGARDFTPVK"
     gene            complement(6380..7441)
                     /locus_tag="GQF42_RS12195"
                     /old_locus_tag="GQF42_12195"
     CDS             complement(6380..7441)
                     /NRPS_PKS="Domain: Aminotran_5 (20-347). E-value: 2.1e-24.
                     Score: 78.0. Matches aSDomain:
                     nrpspksdomains_GQF42_RS12195_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671936.1"
                     /locus_tag="GQF42_RS12195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_12195"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_158919657.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.8e-18, bitscore:
                     64.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METMETFESLVRAEFAPRSIFLNTASSGLLPARTVTALHEAVALR
                     AEGRPLLPLYEDVETCRAAYARLVGVPVTRVAAGASVAAHTGVIAASLPPGAEVLTAED
                     DFTSVLNPFHVRGDLKVRAVPLERIAESVRHGTALVAVSAAQSADGRIADLAALREAAR
                     AHGARTYVDFSQAVGWLPVDADDHDFTATVSFKWLLGPHGAAFLTVPEDFGGLTPLLSG
                     WVAGEDPWDSCYGPVAELAHSARRFDLTHALFTYAGLRRSLELLEELGPDAVRAHDLAL
                     ADRFRAGLADLGHAPVPAPGSAIVSVAGLGGRQPELSRAGIEVSDRAGLLRAAFHLYNT
                     PAEVDRLLDVLAD"
     aSDomain        complement(6401..7381)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GQF42_RS12195_Aminotran_5.1"
                     /evalue="2.10E-24"
                     /label="GQF42_RS12195_Aminotran_5.1"
                     /locus_tag="GQF42_RS12195"
                     /protein_end="347"
                     /protein_start="20"
                     /score="78.0"
                     /tool="antismash"
                     /translation="FLNTASSGLLPARTVTALHEAVALRAEGRPLLPLYEDVETCRAAY
                     ARLVGVPVTRVAAGASVAAHTGVIAASLPPGAEVLTAEDDFTSVLNPFHVRGDLKVRAV
                     PLERIAESVRHGTALVAVSAAQSADGRIADLAALREAARAHGARTYVDFSQAVGWLPVD
                     ADDHDFTATVSFKWLLGPHGAAFLTVPEDFGGLTPLLSGWVAGEDPWDSCYGPVAELAH
                     SARRFDLTHALFTYAGLRRSLELLEELGPDAVRAHDLALADRFRAGLADLGHAPVPAPG
                     SAIVSVAGLGGRQPELSRAGIEVSDRAGLLRAAFHLYNTPAEVDRL"
     gene            complement(7538..8266)
                     /locus_tag="GQF42_RS12200"
                     /old_locus_tag="GQF42_12200"
     CDS             complement(7538..8266)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381151.1"
                     /locus_tag="GQF42_RS12200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_12200"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_158919658.1"
                     /transl_table=11
                     /translation="MRVEIWTDIACPWCYVGKARFDKALAAFPHRDEVEVVHRSFELDP
                     GRAKGDAQPVITMLTRKYGMSEAQAEAGEDNLGAQAAAEGLDYRTRGRDHGNTFDMHRL
                     LHFAKEQGRQDELIQILYRANFAEERSVFTEGDERLVELAVQAGLDAEAARAVLADPSR
                     YAADVRADEREAAQLGANGVPFFVLDRTYGVSGAQPAEVFTQALTQAWGGRSPLKLVQQ
                     GDQGDGAACGPEGCAVPQRG"
     gene            complement(8344..8847)
                     /locus_tag="GQF42_RS12205"
                     /old_locus_tag="GQF42_12205"
     CDS             complement(8344..8847)
                     /EC_number="2.3.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329308.1"
                     /locus_tag="GQF42_RS12205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_12205"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_199272654.1"
                     /transl_table=11
                     /translation="MIHERAGGALVIRTALPAEAEVIADLHFRARSTYYPDGVPRADVD
                     WAEAWRGSVERPDGHVLCAVEQGRIAGIASFRTPDGAPADTVKLFQFHVDPGRWRSGIG
                     TSLHAACVEQWRADRRRTAVLDVHVDNARAQGFYARQGWIPDPENPPADGDHHLFLRFR
                     VPGE"
     gene            complement(8844..9842)
                     /locus_tag="GQF42_RS12210"
                     /old_locus_tag="GQF42_12210"
     CDS             complement(8844..9842)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002239.1"
                     /locus_tag="GQF42_RS12210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_12210"
                     /product="GlxA family transcriptional regulator"
                     /protein_id="WP_233273330.1"
                     /transl_table=11
                     /translation="MHPEVLAMLRVAVVASPPVSMFNLAIPELLFNKVEIDGRPGYEVV
                     ICAPDPGSVPTTGTLGLHIPHGLEAMRQADTVIVAGTGEPYAPDPRVLQVLREVADDGV
                     RIASLCTGAFSLAEAGLLSGRRVTTYWAHADELRRRHPDIEVQGDVLYAQDGQFLTSSG
                     YAAGIDLCLHLIRTDYGAAVANTVARLALVAPVRPGGQTQFTQTPLPPERGTVCADTRG
                     WAMRNLDKPLTLADLARHAGVSVRTLTRRFHAENGVSPLQWLLHQRIERAKELLETTTL
                     PMDQLATACGLGTADSLRAHLVRRTGLTPSAYRAQFSRLGTGRQAVTSSVA"
     misc_feature    complement(10003..10016)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.13"
                     /tool="antismash"
ORIGIN
        1 cggtgtcccc ggccgtggcc gccgcctgcc accacggctc cgcctcggcc tcgtcgccgc
       61 gcacgtgcag gagcgtcgcc agcgtggcca tggcccgggg gtagccgccc tccgcggcgt
      121 tgcgcaggaa ggtctcggcc cgttcgtcgt cgcctgtgtt ccggtagtgc gtgaagaggt
      181 tgaaggcggc cagggccagc ccggcctctg ccgcgtccga ccaccaccgc acggcctcct
      241 cgtccgcgcc ccgtatcttc aggacggcgg cgagattgac ctgggcgacc gggtgtccgg
      301 cctcggccac gggacgcagc agcgcttcga tctcggccgg atcccggtgc tggaacagtt
      361 gtgaggacgt gtagttgatc accgcttcct cgtcgccgga ctcggcgagg gggcgccacg
      421 ccaactcggc gatcgcccac cgctcgccga acgaggcggc ctgggcgatg agcggacgcg
      481 tcgcttcgtc cgcgtacttc agggcggtca gccacaccgg ttccggcacg ttcggcgccg
      541 cggggtcgcg ggccaccgag tcgacgaggt agtcgaaggg tcttttggtg ccttcctcct
      601 caccttccat cagcagactg gcgacgccgt agcggacctg ggaagcccag gcgacggcgt
      661 cggcgaagga ctccgggcgc agcgccgcgc cgcccagggc cttgagatgg tggtggtgca
      721 cctcgtgcag cagggcgacc ggcagatcac cgatcatgcc cacgcgggcc aggtccacgg
      781 cggccgcgac gagtgcggcg cctcgcggat ggccgcgccc ggcggtggat cgccccgccc
      841 tgcgccactc ggccaggagt tccggtccgg ctgccaggta ttcggcgatg ccatgggtgc
      901 catgacgggc atgggcctcg tgcaggcggc tgtcgtcggc ctgcgccacc cgggccagtt
      961 cggcatcgct ccacagccgt cgtacgacga ccggttccac catgttcagc acgcgcagcc
     1021 cggtctcggc ctccattcgg ccgtggccac cggcgcccga gacgtcgtcg gcagcctccc
     1081 gcaggctgtc gtagcgctcg tcgcgcagcg tcgccaggac caccaggcgg gccctgatca
     1141 gcgacgtcag cagggacggc tcgaggcccc cggctcggag gtagtgctcc agatcgtcga
     1201 gccacaggac gtgccggtcg tacaccggct ctccgggcgc ccggcccagc ggtcgcagat
     1261 cggcgcccca gggcggggcg aggaggaggt gtcccgggag caccgcccgc accgcttcga
     1321 gggccgtgcg ggtcttgccg gcggtcgagt cgccggtgag cagcacgaac ccgcccgact
     1381 cggcagccgc ccgcagctcg gcccgcaccc ggccgtccac atcgcgcagg acgtaggggg
     1441 gtagccccga accgtcaccg tccgcggtgc gggcacggtg cacgcccagg ccgaggaggt
     1501 cgacgtcggc cacgggctcc cagtcctgcg cgactgcact cagcgcggca ccggcggtgt
     1561 tgcttgcgcc ggtgccgtag tgcgccacga ccccgccggt ggcgacgagg acatgtcctt
     1621 gatcgatgcc gggcagggaa ggcgaggtgg ggcggttccc gggcacgctc ggctctgccg
     1681 tggggcgggg cgggctcggg gccgccggcc cgttcggcgc cgggtcgcca cccgcacgca
     1741 ggggcgtccc gtgtgcgtgc ctgcgcggcc ggtcctcggc gcggacgtcc acggtgccgc
     1801 ccgcagccag gccgtcctgt ccggccgctg ccgggaccgc gcggtcgtgc tcggccagta
     1861 cggcgcgcaa ctcctcggcg gcgcggaccc gctcgaccgg ttccaggccc tccagcagtg
     1921 cctcgacacg ggcctgccag gccgcctgca gacggctgcg gacggactgc acctgtgccg
     1981 ccgccacgga tcgcagttcg ccggcggtcc ggtccagccg ttcgcgctcc gcccgctcgc
     2041 gctgttcgtc accccgggcg gaccagcgtg ccaccgccgc ccgcagcccg ggccacgtat
     2101 ccgtccccac cgcccgcacc acggcggctg cgccggtctc ggccagcgcc gtccacgcct
     2161 gctccggcat cgccgtcccc cctgcccggg atccctgatc ccaccggcgg accgctgtcc
     2221 cgccagcaca gtagggcgcg ttccccgtcc tgcgcagcgt tacggagaaa cacccgcggc
     2281 ggccggagga cggtgacgta cccggcggaa ccggacgcgg cccgggcacc acccgcgggc
     2341 cgttgtcgta cccgcgtgcc atgctgcggc catgactgct ccgcggaccc cttcggccct
     2401 gcccgacggc ccgtccccct cctgaccgga cccgaactcc ccatgctgga gagccggctg
     2461 gacttccacc gggccacgct ggaactcaag tgccgggggc tggacgacgc gcaggtgcgg
     2521 ctcgccccgg cggagccgtc gtcgccggcg ctgctcgggc tcgtccagca tctcgccgag
     2581 gtcgaacgga actggttcca gcgggtgccg gccggcctgg aggtgccgcc gccgttcgcg
     2641 gacgagacgg ggtacggcct cgatcccgag cgcgggcttc aggaagcgct ggacgtgtgg
     2701 cgggcgcaga tcgcgcgcgg ccgggagctg ctcgccgggc gggagccgga cgacaccggg
     2761 cgcatggccg acaagccgcc ggcaggcgtc gaggtcggtg tgcgccgggt gctcatccac
     2821 ctcgtcgagg agtacgcccg gcacaacgga cacgccgaca ttctgcgtga acggatcggc
     2881 gaaaccaccg gcgcctgaat tcaatttact ttcacaattc cctcctattt cccggacgac
     2941 agcttcccgt cttcttctgc ccgctttttg cagagctaaa cggggcagtt ttcgagcgtg
     3001 tttcttacga gtatctgacg gtttcctggc tgtgacgcac tccacaggtg ggttgatttg
     3061 cccatatttg ccctggtgca atctcgacat ttagccaacc tgcatgcgcg agccagtaac
     3121 ggcggccgtg catttcctaa ttcgcctcgc taaattcgtg ccgcatgact gccgcacccg
     3181 cagaccttct gatcgaccat ccggcggtga acgacggagc cacgctctgg cgtctcgcca
     3241 aggactcgaa aaccctcgac ctgaactcct cgtacagcta tctgctgtgg tgccgcgact
     3301 tcgccgggac ctcggcggtg gcgcgtggtg cggacgggga gccggtcgga ttcgtcaccg
     3361 gctacgtgcg gcccgaccgc ccgcacaccc tgctggtctg gcaggtggcc gtcgactccg
     3421 cccaccgggg tctcggcatc gccgccgcac tgctggacgg gctgaccgca cgactggtcg
     3481 ccgagcgcgg cgtcaccgag gtggagacga ccatcacgcc cggcaacacc gcttccgagc
     3541 ggctgttcac ctcgttcgcc gcccggcacg gggcgcacct cgaacgcgag gtgctgttcc
     3601 ccgccgagct gttcccggac ggcagccacg accccgaagt cctctaccgc atcggcccgc
     3661 tgaccgagtt caccgcccac tgaggagcga ttcaccgtga ccatcaccca gcccgacctc
     3721 agcgtcttcg agaccctcga gtccgaggtg cgcagctact gccgcggctg gcccacggtc
     3781 ttcgaccgcg cgcagggcag ccggatgtac gacgaggacg gtcatgccta cctcgacttc
     3841 ttcgccgggg ccggctcact caactacggc cacaacaacc cggtactcaa acgtgcgttg
     3901 atcgactatc tggatcgcga cggggtcacc cacggcctgg acatgtcgac cacggccaag
     3961 aggtcgttcc tgcaggcctt ccaggatctg gtgctgcgcc cgcgcgatct gccgtacaag
     4021 gtcatgttcc cgggcccgac cggcaccaac gccgtggagt cggcgctcaa gctggcccgg
     4081 aaggtgaagg gccgggaagc gatcgtgtcg ttcacgaacg ccttccacgg gatgtccctg
     4141 ggctccctcg ccgtcaccgg caacgccttc aagcgggccg gagccggcat cccgctggtg
     4201 cacggcaccc cgatgccgtt cgacaactac ttcgacggca cggtcccgga cttcctgtgg
     4261 ttcgagcggc tgctggagga ccagggctcc gggctcaaca agcccgccgc ggtgatcgtg
     4321 gagaccgtgc agggcgaggg cggtatcaac gtggcccggc ccgaatggct gcgcgcgctc
     4381 aaggagttgt gcgagcggca ggacatgctc ctgatcgtcg acgacatcca gatgggctgc
     4441 ggccgtaccg gcgccttctt ctccttcgag gaggcgggca tcgcgcccga catcgtcacc
     4501 gtctccaagt cgatcagcgg ttacgggctg cccatgtcgc tgtgcctgtt caagcccgag
     4561 ctggacgtgt gggagccggg cgagcacaac ggcaccttcc gcggcaacaa ccccgcgttc
     4621 gtcaccgcca ccgcggcgct ggagacgtac tggaccgacg gctcggcgat ggagaagcag
     4681 acccgcgccc ggggcgagca ggtcgagcag gcgctgatct cgatcaccga ggagaacctc
     4741 gccgatgtga aggagtaccg cgggcgcgga ctggtgtggg gcctggagtt ccacgccaag
     4801 gaccgggcgt cgcggatcgc ccggcgggcc ttcgaactcg gactgctcat cgagacgtcc
     4861 ggccctcagg gcgaggtggt caagctgctg ccggccctca ccatcacgcc ggacgagctg
     4921 gacgaggggc tgcgcaccct cgcccgcgcc gtccgcgaaa ccgtctgatc agcaagtcag
     4981 caaggaggaa agacaacacc gtgatcgtcc gatcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcacgtcaa ggcggagtcc ggcacctggg agagcaagcg gatcgtcctc gccagggagc
     5101 gggtcggctt ctccctgcac gagaccatcc tgtacgccgg gacggagacg tcgatgtggt
     5161 acgcgaacca cgtcgaggcc gtcgtctgca ccaagggtgc ggccgaactc accgaccacg
     5221 agaccggccg ggcgtacacc atcacgcccg gcaccatgta cctcctcgac ggccacgagc
     5281 ggcacacgct gcgggtcaag gaggacttcc actgcatctg cgtgttcaac ccgcccgtga
     5341 ccggacggga ggaccacgac gagaacggcg tctacccgct gctcaccgag cccgaggagg
     5401 tgtgacacac gatgaccact gcgcccactg gcttcgtcac cgacctgtac cccagccgcg
     5461 gcaccaccga ggtcggcatc ccccggcagg acccggtcgt ctggggctcc ccggacacgc
     5521 ccggcccgat cccgaccggc gacctgcagt cgtacgagcg tgacggcttc ctcgcgatcg
     5581 accagctgat cacgcccgac gaggtcgagg tctaccggca ggaactggag cggctgatcg
     5641 ccgacccggc gatccgggcc gacgagcgct cgatcgtcga gccggcctca aaggagatcc
     5701 gctcggtctt cgaggtgcac cggatcagca aggtgttcgc gaacctggtg cgcgacgagc
     5761 gggtggtcgg ccgggcgcgg cagatcctcg gctcggacgt ctacgtccac cagtcccgga
     5821 tcaacgtcaa gcccggtttc ggcgcgagcg gcttctactg gcactcggac ttcgagacct
     5881 ggcacgccga ggacggcctg ccgaacatgc gcacggtgtc cgtctcgatc gcactgaccg
     5941 agaaccacga caccaacggc ggcctgatga tcatgccggg ctcgcaccgg acgttcctgg
     6001 cctgcgccgg cgccacgccc aaggacaact acaagaagtc gctgcagatg caggacgcgg
     6061 gcacgccctc ggacgaggcg ctgaccaaga tggcgggcgc gtacggcatc aagctcttca
     6121 cgggcaaggc cggttcggcg acctggttcg actgcaactg catgcacggc tccggcgaca
     6181 acatcacgcc gttcccgcgc agcaacgtct tcatcgtgtt caacagcgtg gagaacgcgg
     6241 ccgtcgagcc gttcgcggca ccggtccgca ggccggagtt catcggggcc cgggacttca
     6301 ctccggtgaa gtagcccctt gagaacaccg ggccggggcc tccgcgtgtg ggacgggggg
     6361 ctccggcccg gccttgtcgt cagtcggcga gcacgtccag cagacggtcg acctccgcgg
     6421 gcgtgttgta gaggtggaag gccgcgcgga gaaggcccgc gcggtcggag acctcgatgc
     6481 cggcgcggct cagctccggc tgccggccgc cgagtccggc aacggagacg atcgccgagc
     6541 cgggggcggg caccggcgca tggccgaggt ccgcgagtcc ggcccggaac cggtcggcga
     6601 gcgccaggtc gtgggcgcgt acggcgtccg ggcccagctc ctcgagcagt tcgagggagc
     6661 ggcgcagccc ggcgtaggtg aacagggcgt gggtgaggtc gaaccgccgg gcggagtggg
     6721 cgagttcggc gacggggccg taacagctgt cccaggggtc ctcgccggcg acccagcccg
     6781 acagcagggg ggtgaggccg ccgaagtcct cgggcacggt gaggaaggcg gcgccgtgcg
     6841 ggccgagcag ccacttgaag gagacggtgg cggtgaagtc gtggtcgtcc gcgtccaccg
     6901 gcaaccagcc cacggcctgg gagaagtcga cgtaggtacg ggcgccgtgc gcgcgggcgg
     6961 cctcgcgcag ggcggcgagg tcggcgatcc gcccgtcggc ggactgggcc gcgctgaccg
     7021 cgaccagcgc cgtgccgtgg cgcacggact cggcgatccg ctccagcggg accgcccgca
     7081 ccttgaggtc gccgcgcaca tggaacgggt tcagtacgga ggtgaagtcg tcctcggcgg
     7141 tgaggacttc ggcacccggg ggcagcgagg cggcgatcac accggtgtgg gcggcgaccg
     7201 aggcgcccgc cgcgaccctg gtgaccggaa caccgacgag ccgggcgtag gcagcccggc
     7261 aggtctcgac gtcctcgtag agcggaagca gcggcctgcc ctcggcccgc agggccaccg
     7321 cctcgtgcag cgcggtgacc gtgcgggccg ggagcaggcc gctgctcgcg gtgttcagaa
     7381 agatgctcct gggggcgaac tcggcgcgga cgaggctctc gaacgtctcc atggtctcca
     7441 tgggtccact gtgcggcccg gtgaagtcac ccgtccattg cgcaaaaaga catggatcct
     7501 ctcagcaggg cttatacgtc cgcgccgacc tgcggtttca gccccgctgc ggcaccgcgc
     7561 acccctccgg tccgcaggcc gccccgtccc cctggtcccc ctgctggacg agcttgagcg
     7621 gggagcgccc gccccaggcc tgggtcagcg cctgggtgaa gacctcggcg ggctgggcgc
     7681 cggagacgcc gtacgtgcgg tcgaggacga agaacggcac accgttcgca ccgagctggg
     7741 cggcctcgcg ctcgtcggcg cggacgtcgg cggcgtagcg ggacgggtcg gcgagcaccg
     7801 cgcgggcggc ctcggcgtcg aggccggcct gcacggccag ctcgaccagc cgctcgtcgc
     7861 cctcggtgaa gacggaccgc tcctcggcga agttcgcccg gtacaggatc tggatcagct
     7921 cgtcctgcct gccctgctcc ttggcgaagt gcagcaggcg gtgcatgtcg aaggtgttgc
     7981 cgtggtcacg gccccgggtg cggtagtcca ggccctcggc ggcggcctgg gcgcccaggt
     8041 tgtcctcgcc ggcctcggcc tgggcctcgc tcatgccgta cttcctggtg agcatggtga
     8101 tcaccggctg ggcatcgccc ttggcgcggc ccgggtccag ttcgaaggag cggtgcacca
     8161 cctcgacctc gtcgcggtgc gggaaggcgg cgagcgcctt gtcgaaccgc gcctttccca
     8221 cgtagcacca gggacaggcg atgtccgtcc agatctcgac gcgcatgctc tcggctcttc
     8281 tccgggtcgt accggcgcga aggctctccc cgcgccggta cgtgaacgtt caagcagctc
     8341 tgttcattcc ccgggcaccc gaaagcggag gaacaggtga tggtcaccgt cggcgggcgg
     8401 attctccggg tccgggatcc agccctggcg ggcgtagaaa ccctgggcgc gcgcattgtc
     8461 cacgtgcacg tccagcacgg ccgtgcgtcg cctgtcggcg cgccactgct ccacgcaggc
     8521 cgcgtgcagg gacgtaccga tgccggagcg ccagcggccg gggtcgacgt ggaactggaa
     8581 cagcttgacc gtgtcggccg gtgcgccgtc cggggtacgg aaggaggcga tgccggcgat
     8641 ccggccctgc tcgaccgcgc acagcacatg gccgtcgggg cgctcgacgg agccgcgcca
     8701 ggcctcggcc cagtcgacgt cggcccgcgg gacaccgtcc gggtaatacg tcgagcgggc
     8761 ccggaagtgc agatcggcga tgacctcggc ctcggcgggc agggcggtgc ggatcaccag
     8821 ggcgccgccg gcacgttcat ggatcatgca acggaggacg tgaccgcctg ccgcccggtt
     8881 ccaaggcggc tgaactgggc ccggtaggcg ctcggggtga ggccggtgcg gcgcaccagg
     8941 tgggcgcgga gcgagtcggc ggtgccgagg ccgcaggcgg tggccagctg gtccatgggc
     9001 agggtggtgg tctccagcag ttccttggcg cgctcgatgc gctggtgcag cagccactgc
     9061 agcgggctga cgccgttctc ggcgtggaag cgccgggtga gggtgcgcac cgagacgccc
     9121 gcgtggcggg cgaggtcggc gagggtgagc ggcttgtcga ggttgcgcat ggcccagccg
     9181 cgggtgtcgg cacagacggt gccgcgctcg ggcggcagcg gggtctgggt gaactgggtc
     9241 tggccgccgg ggcggaccgg ggcgaccagg gcgaggcggg cgacggtgtt ggcgacggcg
     9301 gcgccgtagt cggtgcggat gaggtgcagg cacaggtcga tgccggccgc gtagccggag
     9361 gaggtgagga actggccgtc ctgggcgtag aggacgtcgc cctggacctc gatgtcgggg
     9421 tgccggcgac gcagctcgtc ggcgtgcgcc cagtaggtgg tgaccctgcg cccgctgagc
     9481 agtccggcct cggcgagcga gaacgcaccg gtgcacaggg aggcgatgcg cacgccgtcg
     9541 tcggcgacct cgcgcagcac ctgaagcacc cgcggatcgg gcgcgtaggg ctcgccggtg
     9601 cccgcgacga tcacggtgtc cgcctgccgc atcgcctcga ggccgtgcgg aatgtgcagg
     9661 ccgagggtgc cggtggtggg cacggacccg gggtcgggcg cgcagatcac cacctcgtag
     9721 ccgggccgtc cgtcgatctc gaccttgttg aacagcagct cggggatggc gaggttgaac
     9781 atcgagacgg gcggggacgc gacgaccgcg acacgcagca tggccagaac ctccggatgc
     9841 atggcattca ggccactact gtacgcggac gaagatccgc accatgaagg catgccaacc
     9901 aagcagaccc tcgacacatc ccaacccggc tccgccacaa gccggttgcc gaacaacccc
     9961 gcccccgccc tcaccctgac cgcagccgtg ctgggctttg cgttgatcac cctcgacgcg
    10021 tccgtggtca ccgtggccct cccggcgatc ggctccgcgc tcggcggcgg catgtccggc
    10081 ctgcagtggg tggtcgacgc ctacacgctg gccttcgccg ccctgatgct ctccacgggc
    10141 gccttcgcgg accgggccgg ggcgagccgg gcgtacgccc ttggcatcgc cgtgttcacg
    10201 ctcgcctcgg cggcctgcgg tctggcgccg acgctcccgg tgctgatcgg cgcgcgcgtg
    10261 gtgcagggtg tcgcggcggc cgtcgtgctg cccgcgtcgc tgtccctggt acggcaggcc
    10321 taccccgatc cggcgcaccg cgcgaaggcg gtggcgctgt gggccgccgg cggctcgatc
    10381 gcggtggcgc tgggcccggt ggcgg
//
