LOCUS       NZ_CP047020            22784 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces broussonetiae strain T44 chromosome.
ACCESSION   NZ_CP047020
VERSION     NZ_CP047020.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces broussonetiae
  ORGANISM  Streptomyces broussonetiae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9735779)
  AUTHORS   Mo,P.
  TITLE     Streptomyces sp. strain T44 isolated from rhizosphere soil of
            Broussonetia papyrifera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9735779)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) College of Life Science and Technology,
            Central South University of Forestry and Technology, No. 498,
            Shaoshan South Road, Changsha, Hunan 410004, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2019
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009796285.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:56:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,022
            CDSs (total)                      :: 8,931
            Genes (coding)                    :: 8,515
            CDSs (with protein)               :: 8,515
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 416
            CDSs (without protein)            :: 416
            Pseudo Genes (ambiguous residues) :: 0 of 416
            Pseudo Genes (frameshifted)       :: 171 of 416
            Pseudo Genes (incomplete)         :: 334 of 416
            Pseudo Genes (internal stop)      :: 29 of 416
            Pseudo Genes (multiple problems)  :: 110 of 416
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:31:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1676345
            Orig. end                         :: 1699129
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047020.1.
            Bacteria and source DNA available from Zhao YL.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22784
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="7"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22784
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22784
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12784](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12784
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     gene            368..940
                     /locus_tag="GQF42_RS07930"
                     /old_locus_tag="GQF42_07930"
     CDS             368..940
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962957.1"
                     /locus_tag="GQF42_RS07930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_07930"
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="WP_158929913.1"
                     /transl_table=11
                     /translation="MSAAGRRRRETVRNQAAELFEQKIKPLEVARRLRVSPKSAYLWHQ
                     LWRDGGVRALASRGPSGSRCRLSPRCLEKLAAYLEEGPAAHGWDQDQVWTGARVATLIG
                     RKFHVSYSVSGATRLMRRLGFTPQVPARRAAERDEQAIAVWKEATWGEVKAPGRPVTAS
                     SASRTKPGSPAGRRRAAPGAGGASPRS"
     gene            856..1425
                     /locus_tag="GQF42_RS07935"
                     /old_locus_tag="GQF42_07935"
     CDS             856..1425
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1238: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655131.1"
                     /locus_tag="GQF42_RS07935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_07935"
                     /product="transposase"
                     /protein_id="WP_158929911.1"
                     /transl_table=11
                     /translation="MCFEDEAGFTRRPPKGRTWGRRGITPVVRVSGRRSGRVSVAGPIA
                     TRPGSRTRLCNRLRLHTGRKGERCSLSEDDYIRLIDGMHQLVKAPIVLVWDRLNTHVSH
                     TMRELIEAREWLTVFILPSYAPELNAVEYLWAHVKRSLANLASVALDRLEALVRNRLKR
                     LQYRPDLLDGFVAGTGLSLDLPPPSP"
     gene            complement(1838..2452)
                     /locus_tag="GQF42_RS07940"
                     /old_locus_tag="GQF42_07940"
     CDS             complement(1838..2452)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GQF42_RS07940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GQF42_07940"
                     /product="hypothetical protein"
                     /protein_id="WP_158918942.1"
                     /transl_table=11
                     /translation="MAAELLVDCAAVAAGVVQRVLRLLPRAVPAAVAALELERELRRPL
                     MRRVGPVRTDLLLSLAGATVNGLCAGIGMPAVDAAHRLLLLGEVRARRRVWERRERDLC
                     SGPECVPRTRPARHLRARTRPPGPVEQWERRLAPSAPLTAVAVLALRGSPRRAADALLA
                     TVPRAARYGREAFAAMVGRELARHASYRSTRRRAACCLRRG"
     gene            2694..2918
                     /locus_tag="GQF42_RS07945"
                     /old_locus_tag="GQF42_07945"
     CDS             2694..2918
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012780.5"
                     /locus_tag="GQF42_RS07945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_07945"
                     /product="CBS domain-containing protein"
                     /protein_id="WP_055706085.1"
                     /transl_table=11
                     /translation="MADLVTWRSQFGPPVTVRPDTALGEAADQLVDSRATSLLVVDDSD
                     RPVGRILADGLLDYLLPERGRLHFRRFLR"
     misc_feature    complement(2695..2709)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.18"
                     /tool="antismash"
     misc_feature    2706..2721
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            3113..4204
                     /locus_tag="GQF42_RS45750"
                     /old_locus_tag="GQF42_07950"
                     /pseudo=""
     CDS             3113..4204
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013715.5"
                     /locus_tag="GQF42_RS45750"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_07950"
                     /product="NRAMP family divalent metal transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLLAIALVMVQEMAVRLGAHTGKGLGALIREQFSLRLTGLAVFCL
                     LLANTGLVVSEFAGIGAAFELLGVPKWAVIPPAAILLWSLVLFGSYRWAERIFLIMSPA
                     FFAYPIAMILGHPH"
     gene            complement(<4523..6388)
                     /locus_tag="GQF42_RS45755"
                     /pseudo=""
     CDS             complement(<4523..6388)
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF018884.5"
                     /locus_tag="GQF42_RS45755"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="SpoIIE family protein phosphatase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MMRASRETGHHSPFDTPTAAMIVVDARGRVTGWSPGASSLYGADT
                     RDVLDRPVSEVLRDVRARADTLQGFEAPGAPASGGARTETRTVRRPDGSLAETVLRIFP
                     VEQGQGRPAWVVMAVASDRYEERMTDQSMLSRLFTQAPVGLSVYGPDTRLKWVNEAVQE
                     AMGWHAGEWFGRPHIVLYPHGKILSPPGYHDMDEVLNEVLSSGEPVLDVHWRGPTPAAS
                     EYYQVYSCSYFRLQDEAGHPLGICEAAVDVTDRYEAQTRLALLGRASGIGTTLDARQTA
                     EELAGLVVPEFADSVRIEVAESVLGGEEVPSYASGAPAGLRLMTQRTKSNSDITNSDVL
                     LGAIGIRSTTAHQRLLALPLLLGSTTLGTVTLLRQPPRAPFTEEDRALAEELTSRTAVC
                     LDNARRYIREHTTALKLQRDLLPRALPQSSGIEVADRYIPAAGPLGVGGDWYDVIPLSG
                     ARVGLVVGDVVGHGVDAAATMGRLRTSVRALAALDLPPDELLARLDDLVGQTRVGVHRT
                     PGDEGTDRALGATCLYAVYDPTSCTCAMASAGHLPPVAAGRGAGARQAEVLDLPIGPPL
                     GIGGLPFESAEFEFAEGTVLALFTDGIVKARNRDVDEGVADLCDAL"
     misc_feature    6387..6403
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            complement(6703..8262)
                     /gene="mftG"
                     /locus_tag="GQF42_RS07965"
                     /old_locus_tag="GQF42_07965"
     CDS             complement(6703..8262)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene="mftG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379782.1"
                     /locus_tag="GQF42_RS07965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_07965"
                     /product="mycofactocin system GMC family oxidoreductase
                     MftG"
                     /protein_id="WP_233273288.1"
                     /sec_met_domain="DAO (E-value: 2.5e-07, bitscore: 29.6,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAETTDRTSADVIVVGAGASGAALAARLSEDAGRTVLLVEAGPFP
                     RRLRDFALELLDARLIPGAQPDHRAVQRYAAHLTPERPYTVVRGRHLGGSTTVNGGYFV
                     RARRADFDRWAAAGNPAWTYDRMLPWLRALETDLDHGADDLHGGCGPVLVRRASLAHPA
                     AVAFRDAADHLGHPLEPDKNDQAPPGFGPVPCNAVDGVRLNTGLTYALSALGRPNLTAL
                     GDCPVSRIVIRNGRATGVAVRQNGQETVFCGGEIVLCAGSLVTPQLLQLSGIGPRADLE
                     RLGIPVAVDAPAVGTRFSDHPQLVLEWSPRRELGAPHGSWLGGCLHLSSSDGTHPGDLE
                     ILQSLVPLAGLVGGTTTVPGAPLPFLVSVQSPGRTGRLSLRSADPATPPRIEYGYLASP
                     KDRRRLREAVRATAELITTPAFDEISHGLLQPGPDVLNCDHALDGWINRNIGTAHHTCG
                     TAPMGPDTANAVVDQYGRVHGVHGLRVADTSILPTAPLRGPAATAVLIGEVIAHAMRAE
                     LP"
     gene            complement(8255..9691)
                     /gene="mftF"
                     /locus_tag="GQF42_RS07970"
                     /old_locus_tag="GQF42_07970"
     CDS             complement(8255..9691)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076812.1"
                     /locus_tag="GQF42_RS07970"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969).; Members of this protein family, MftF,
                     are glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_07970"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_233273289.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 6.1e-21,
                     bitscore: 74.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDVPPLPAVFRLRPDPGLRLLRDGRVLLGGSPYRLMRLTARAVP
                     VVSAWLDGKPVGEGRATGLLARRLVQAGMMHPVPPSEAPRPSVTVVVPVRDRAGHLARC
                     LAGLSGGPEVIVVDDASADPGAVADVVRAAGARLVRRPVNGGPAAARNTGLAHATTELV
                     AFVDSDCLPTRAWADALLPHFADPAVAAVAPRIVAEGAGRGFLAAYECARSALDMGPRE
                     SLVRPGSHVPYVPSAALVVRRTAVGRGFDETMRVGEDVDLVWRLAADGHHVRYDPSATV
                     AHRHRTRPHQWLAQRIQYGTSAAPLAARHPGTLPATSMAGWSALAWGLALARRPLAGAL
                     VTAGTTGLLARRLAAWSDRPWATAARLSGGGTLLAGEQLGRAVTRTWWPLALPLAAAVP
                     RLRLPLAAAALLPPLLEHRRSRPGLPVLPWTVTRLADDLAYSLGVWKGCIRERTTEPLR
                     PRLWWLSADGVTPPSGRTGG"
     misc_feature    9755..9770
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 21.84"
                     /tool="antismash"
     gene            9869..9988
                     /gene="mftA"
                     /locus_tag="GQF42_RS07975"
                     /old_locus_tag="GQF42_07975"
     CDS             9869..9988
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379790.1"
                     /locus_tag="GQF42_RS07975"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="GQF42_07975"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_158918944.1"
                     /transl_table=11
                     /translation="MTDNSQTSTADRLPAEDDQLIEEDLLVEDVSIDGMCGVY"
     gene            10001..10342
                     /gene="mftB"
                     /locus_tag="GQF42_RS07980"
                     /old_locus_tag="GQF42_07980"
     CDS             10001..10342
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03967.2"
                     /locus_tag="GQF42_RS07980"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="GQF42_07980"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_158918945.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 4.7e-28,
                     bitscore: 96.3, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTHRNGSERFDLEAAWAVHPQVAVRPEPFGALLYHFGTRQLSFL
                     KDRRLLTVVQELAHRPSAAEACRVAGVDPAESGRYRDALAQLAKSRMITERTGPPSRGD
                     AHLQGEDAR"
     aSDomain        10043..10279
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_GQF42_RS07980_Mycofactocin_RRE.1"
                     /evalue="1.50E-30"
                     /identifier="RREFam016.1"
                     /label="GQF42_RS07980"
                     /locus_tag="GQF42_RS07980"
                     /protein_end="93"
                     /protein_start="14"
                     /score="96.3"
                     /tool="antismash"
                     /translation="AAWAVHPQVAVRPEPFGALLYHFGTRQLSFLKDRRLLTVVQELAH
                     RPSAAEACRVAGVDPAESGRYRDALAQLAKSRMI"
     gene            10339..11553
                     /gene="mftC"
                     /locus_tag="GQF42_RS07985"
                     /old_locus_tag="GQF42_07985"
     CDS             10339..11553
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671058.1"
                     /locus_tag="GQF42_RS07985"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="GQF42_07985"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_158918946.1"
                     /sec_met_domain="PTHR43273 (E-value: 1.4e-38, bitscore:
                     131.8, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2.1e-23, bitscore: 82.5,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 3.6e-07, bitscore: 28.9,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.6e-14, bitscore:
                     53.1, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 2.6e-06, bitscore: 26.8,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVPSVAPPSPARTGRLVDHFQHGLEAPICLTWELTYACNLACVH
                     CLSSSGRRDPRELTTDECKMVIDELERMQVFYVNIGGGEPTVRPDFWELVDYATAHQVG
                     VKFSTNGVRITPDAARRLAASDYVDVQLSLDGATAEVNDAVRGEGSYATVLQAMENLAA
                     AGFAGFKISVVATRHNIGQLDAFKALADRYGAQLRLTRLRPSGRGADVWDELHPAAAQQ
                     RQLYDWLVEHGDEVLTGDSFFHLSAYGEALPGLNLCGAGRVVCLIDPVGEVYACPFAIH
                     DAFRAGNVRDEGGFTRVWRSSSLFQELRQPQTGGACTKCSAFDACRGGCMAAKFFTGLP
                     LDGPDPECVQGHAEAALAGDRSSAPRPSEDHSHRGPVRGQPVLLGLPVRRESRPTRMCD
                     ESPLA"
     gene            11591..12784
                     /gene="mftD"
                     /locus_tag="GQF42_RS07990"
                     /old_locus_tag="GQF42_07990"
     CDS             11591..12784
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379794.1"
                     /locus_tag="GQF42_RS07990"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="GQF42_07990"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_158918947.1"
                     /sec_met_domain="TIGR03966 (E-value: 1.7e-217, bitscore:
                     720.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPNAWFETVAEAQRRAKRRLPASVYGALLAGSERGTTYQDNMAAF
                     AEAGFAPHVADLPAKRDQATTVLGQSISLPVIISPTGVQAVHPNGEVAVARAAAARGTA
                     MGLSSFASKPVEEVVAANPQTFFQMYWMGGKDSMIAQVERAREAGAKGLIVTLDWSFSH
                     GRDWGSPHIPERLDLKAMARFAPEGITHPRWLLAFARTRRLPDLTTPNVAAVGEQAPTF
                     FGAYGEWMQTPPPTWDDVRWMREQWGGPFLVKGIIRVDDAKRAVDAGAQAVSVSNHGGN
                     NLDSTPATIRALPAIADAVGGQIEVLLDGGVRRGSDVVKALALGARAVMIGRAYLWGLA
                     AGGQAGVENVLDILRGGIDSALLGLGRSSIHDLTPDDVVLPDGFARVLGAPRPAQAP"
     gene            12784..13482
                     /gene="mftE"
                     /locus_tag="GQF42_RS07995"
                     /old_locus_tag="GQF42_07995"
     CDS             12784..13482
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: protein motif:HMM:TIGR03964.1"
                     /locus_tag="GQF42_RS07995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_07995"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_158918948.1"
                     /transl_table=11
                     /translation="MTTLASLTWTDVADLAGRGTVLAVPVGATEQHGPHLPMSTDTDIA
                     VALTDRLAAQAPDVVVAPAVAFGSSGEHQDFPGTLSVGQEAVELLLVELVRSATVTFPR
                     VVLVSAHGGNAQPVTRAVRRLGTEGRHVLAWGPRWGGDAHAGRTETSVMLALAPHLVRM
                     EAARAGNTAPVHTLLPRLRDAGLSAVTANGVLGDPGGANPDEGRLLIQRAVAELADLVT
                     RHRRHTDAAS"
     gene            complement(13564..14226)
                     /locus_tag="GQF42_RS08000"
                     /old_locus_tag="GQF42_08000"
     CDS             complement(13564..14226)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012301.5"
                     /locus_tag="GQF42_RS08000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_08000"
                     /product="response regulator transcription factor"
                     /protein_id="WP_158918949.1"
                     /transl_table=11
                     /translation="MTLVLAERVAPTGRVRPVLEDRCQMQRVLVAEEHELVLAGLRAVL
                     LSQPWVASCLAADSQEAAWQVARRHHPQLVLVSTSLGGRSGLDLCRDLRDRMPHVKVVL
                     LSGEGRVSAALASAHGAVASLSKQMPTAAIVAAVKRVAEGARVFPKDEAPAAARLSKRE
                     LDVLQHLVLGLSNPEVAAVLNLSRHTVKQHTTTVYRKLGVRNRAQAASRAQELGLVG"
     gene            14551..16857
                     /locus_tag="GQF42_RS08005"
                     /old_locus_tag="GQF42_08005"
     CDS             14551..16857
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024793.5"
                     /locus_tag="GQF42_RS08005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_08005"
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_158918950.1"
                     /transl_table=11
                     /translation="MPTPNAVPAELTSFVGRRKDVAALRQLFSTTRLVTLTGIGGVGKT
                     RLALRVAGEMQRAFPDGVCLVELASLKDPALLPHSVVDALGIKEQSPRSPMTVLRDHLG
                     QRRMLLVLDNCEHLVDAAAGLADQVLRAAPEVRILATSRQALRIAGEHIYPVPPLPTPP
                     PDESVEPGTATQYPSVTLFADRSAAVVPGFSITPDNEAAVVRLCRRLEGIPLAIELASV
                     RLRVLTVHDLASRLDDRFQLLREGTRNLPERHQTLQALIDWSYDLCTPNEQRLWARASV
                     FVGGFSTDALEAVCTDEALPRHAVLDTVAGLVDKSIFIREEQGEYVRFRMLETIRAYGQ
                     ARLSESGLEPALRRRHRDRYRQLMEVAGDEWSGPRQREWASRLQLELPNLRHALEYCLS
                     QPGEARTGLRMAAAPWLWLAMGYPTEARLWLGRALALDAEPSRERAWALATVAYIAACQ
                     GDEAAAAESAEDARKVAVHLNDPAALAYATHVRGLSKFLSTDLSGAIPLFNEALEQYAE
                     TKTPVHYPNNLRTELAAALILLGELDKAAEIIEELFRRCTAAGDQWVLSWVLWGRGFLR
                     LIHGELDAAEADLCEALRIKRFFHDTLGMALALDVLAWTTAAKGNAERAATLFGGANPL
                     WQTLGAPLFGWTLLLAQRAKFETMARDKMGDAQFDAALERGSALTTEETLALALRERTQ
                     PASDAPRPASTLTRREREVAELVAQGLSNKDIAAQLVISLRTAEGHVEKILTKLGFNTR
                     TQIASWVVQRQSERT"
     gene            17031..17462
                     /locus_tag="GQF42_RS08010"
                     /old_locus_tag="GQF42_08010"
     CDS             17031..17462
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432607.1"
                     /locus_tag="GQF42_RS08010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_08010"
                     /product="ATP-binding protein"
                     /protein_id="WP_158918951.1"
                     /transl_table=11
                     /translation="MAPSGIAGARPSLGREARQWDLPHAPQAAGIARHLALDTLRGWGV
                     REDQAHQVVLAVSELVTNAVEHALPPVALQLVPPNEHQTIHVAVTDGGPANDRGARTAD
                     CEPDEHGRGSAIIGFLASARGTRMCGRSATHWADLPLTA"
     gene            17703..19046
                     /locus_tag="GQF42_RS08015"
                     /old_locus_tag="GQF42_08015"
     CDS             17703..19046
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241812.1"
                     /locus_tag="GQF42_RS08015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_08015"
                     /product="MFS transporter"
                     /protein_id="WP_233273290.1"
                     /transl_table=11
                     /translation="MTNAVAFMLPPLLPLMQQQFGLGVAASAWIFTALTLGGGAGFVLL
                     PRLTDVLNDRTTSLLSGGFLVVGALVPAIGDSYPALLVGSALLGFGGAAQLLPLSFLRR
                     HLTGSAVATAVSVLIMATGSGTVLGMVGGGLTVKYLSLASFFYILAAAFAVTTVALLII
                     VPSSRPESSGRIGILGTVWLIAWVTAVLLALTQGLTWSGTAILAVLGAGVVGGVVWGLV
                     QRKSPTAVFDIQLLKKPLVTPACFAAGIFGAIDAAFLLLVTYYTQTPAGVGYGLGVDAL
                     GTGLLMLPFALMMFVGGKAAERAVQKGRPATVLVIGTAVSVVGLGWLAFAHDQRWQYLV
                     GAAFVGLGSRVGYSGAFAVPQLVVPEDKAGMAAGMAGTTMAIGFAFGSAVVTTLLTFDT
                     IPKIGLPKEYLYTVGYEVTVGLSLLVLVVTLISRLRRGGELTASSVEA"
     gene            19247..19711
                     /locus_tag="GQF42_RS08020"
                     /old_locus_tag="GQF42_08020"
     CDS             19247..19711
                     /EC_number="3.1.2.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 4HBT_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024675.5"
                     /locus_tag="GQF42_RS08020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_08020"
                     /product="thioesterase family protein"
                     /protein_id="WP_158918953.1"
                     /sec_met_domain="4HBT_2 (E-value: 4.7e-14, bitscore: 51.9,
                     seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVPGNAAKRHIIAVNGSDVWRSEFRLRFGDFDGLGHLTASVYLA
                     FFEETRSAWMTGTLGVAYPTYVVATQHIEYLHEVTPDDGAVSVELALREVRTSSFRVVE
                     HLTTAGYLCARSTATLAMWDMERRQSRPITPDERAVFEAYLAEPTHPMTP"
     gene            19847..21064
                     /locus_tag="GQF42_RS08025"
                     /old_locus_tag="GQF42_08025"
     CDS             19847..21064
                     /EC_number="2.3.1.9"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1124: acetyl-CoA acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006125100.1"
                     /locus_tag="GQF42_RS08025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_08025"
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="WP_158918954.1"
                     /transl_table=11
                     /translation="MPEAVIVATARSPIGRAGKGSLREMRPDDLTVQMVRAALAKVPQL
                     DPTEIDDLVLGCGQPGGESGFNMARIVAVLAGLDSVPGTTVNRYCSSSLQSTRMAMHAI
                     KAGEGDVFISAGVETVSRFFKGNADGLPDTENPVFAEAQARTEKLASGGGTWVDPRTEG
                     ALPDVYIAMGQTAENVAQSTGVTREDQDAFAVRSQNLAEQAIADGFWARDITPVTLPDG
                     SQIVRDDGPRPGVTLEAVSALEPVFRPDGTVTAGNCCSLNDGAAALVIMSDTKAKQLGI
                     TPLARIVSTGVTGLSPEIMGLGPVEASKQALRRAGLTIDDIDLVEINEAFAAQVIPSAR
                     ELGIDFDRLNVNGGAIAVGHPFGMTGARITSTLINSLQYHDKQFGLETMCVGGGQGMAM
                     VLERLS"
     gene            <21063..21623
                     /locus_tag="GQF42_RS08030"
                     /old_locus_tag="GQF42_08030"
                     /pseudo=""
     CDS             <21063..21623
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003957185.1"
                     /locus_tag="GQF42_RS08030"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_08030"
                     /product="SDR family oxidoreductase"
                     /pseudo=""
                     /sec_met_domain="adh_short_C2 (E-value: 1.2e-30, bitscore:
                     106.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DQALRRQVVTRALDSFGSADLLVNNTGINPVYGPLMTLDLDVARK
                     IVEVNCLAALSWTQQMYRAWMSAHGGAVVNVSSVAAVRPAPGIGLYGASKAMLTHLTEE
                     LAVELGPDIRVNAVAPAVVKTKFATALYDGREDEAAAAYPLKRLGVPEDIGGTVAFLLS
                     DDAAWITGQVLVVDGGVTLTGAG"
     gene            21658..22413
                     /gene="fabG"
                     /locus_tag="GQF42_RS08035"
                     /old_locus_tag="GQF42_08035"
     CDS             21658..22413
                     /EC_number="1.1.1.100"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (5-165). E-value: 1.6e-18. Score:
                     59.2. Matches aSDomain:
                     nrpspksdomains_GQF42_RS08035_PKS_KR.1"
                     /codon_start=1
                     /gene="fabG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658331.1"
                     /locus_tag="GQF42_RS08035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GQF42_08035"
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="WP_158918955.1"
                     /sec_met_domain="adh_short (E-value: 3.9e-36, bitscore:
                     123.7, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTRTAIVTGAARGIGADVAQRLAADGLAVAVVDLDESACKPVV
                     ERIEAAGGRALAVGVDVSDEPGVQTAVQRIAEELGDPTVLVNNAGIIRDNLLFKMTATD
                     WDAVMNVHLRGSFLMSRGVQSYMTKAGWGRIVNLSSTSALGNKGQANYAAAKAGLQGFT
                     KTLALELGKFGVTANAIAPGFIETEMTAATAQRMGMSFDDFRKAVTAQIPVARSGLPKD
                     IAHAVSFFVSEDAGYVSGQVLYVAGGPQA"
     aSDomain        21673..22152
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GQF42_RS08035_PKS_KR.1"
                     /evalue="1.60E-18"
                     /label="GQF42_RS08035_PKS_KR.1"
                     /locus_tag="GQF42_RS08035"
                     /protein_end="165"
                     /protein_start="5"
                     /score="59.2"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TAIVTGAARGIGADVAQRLAADGLAVAVVDLDESACKPVVERIEA
                     AGGRALAVGVDVSDEPGVQTAVQRIAEELGDPTVLVNNAGIIRDNLLFKMTATDWDAVM
                     NVHLRGSFLMSRGVQSYMTKAGWGRIVNLSSTSALGNKGQANYAAAKAGLQGFTKT"
     CDS_motif       21679..21741
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GQF42_RS08035_0001"
                     /evalue="6.00E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="GQF42_RS08035"
                     /protein_end="28"
                     /protein_start="7"
                     /score="12.5"
                     /tool="antismash"
                     /translation="IVTGAARGIGADVAQRLAADG"
ORIGIN
        1 tccacctcat cacgcgcggc ccatttgtag taattgcccg attcgtcacg ctggcgccga
       61 cttcctgagc cgcccttctg gaccagtcac ggaccaaaca gcgggccacc tggaggcggc
      121 aagccaatcg ccagaggcag ggcatcgaac aatgcacggt tcggtgcgag ccacccgtcc
      181 ccggcagcga cggcggtagg gatctgcccg atcccaccgg ggacggccag gccggaaaga
      241 ccggctacag cgccacgatg accatttact gactgcggct cgtcagggtg aactcgctcg
      301 aagtccctga tgggctggca gtgctggctg tatctggtgg tgtgtgagat atggggatgg
      361 gggcgggctg tctgctgcgg gacggcggcg ccgggagacg gtacggaatc aggcggccga
      421 gctgttcgag cagaagatca agccgctgga ggtggcacgg cgcctgcggg tgagcccgaa
      481 gtcggcctat ctatggcatc agctatggcg ggacggcggt gttcgggctc tggcctcccg
      541 tggcccgagc ggatcgcggt gccggctgtc cccgcgctgc ctggagaagc tggccgcgta
      601 cctggaggag gggccggctg cgcacggctg ggaccaagac caggtgtgga ccggtgcgcg
      661 ggtggcgacg ctgatcggca ggaagttcca cgtctcctac agcgtctcgg gggccacccg
      721 gctgatgcgc cggctcgggt tcaccccgca ggttcctgct cgcagagccg cggaacgcga
      781 cgagcaggcc atcgccgtgt ggaaggaggc gacgtggggg gaggtaaaag cgcccgggcg
      841 gcctgtgacg gcttcatctg cttcgaggac gaagccgggt tcacccgcag gccgccgaag
      901 ggccgcacct ggggcaggcg gggcatcacc ccggtcgtga gagtgtccgg ccgccgttcg
      961 gggagggtct ccgtcgccgg gccgatcgcg acgcgtccgg gctcgaggac ccggctgtgc
     1021 aaccggctgc ggctgcacac cggacgcaag ggcgagcgct gcagcctgtc cgaggacgac
     1081 tacatccgtc tgatcgacgg gatgcaccag ctggtcaagg caccgatcgt gctcgtatgg
     1141 gaccggttga acacccacgt ctcccacacc atgcgcgagc tgatcgaggc acgggagtgg
     1201 ctgacggtgt tcatcctgcc ctcctatgca cccgaactga acgcggtcga gtacctgtgg
     1261 gcccacgtca aacgcagtct cgccaacctc gcgagcgtgg ctctcgaccg gctcgaggcc
     1321 ctcgtccgca accgactcaa acgcctccag taccgcccgg atctcctcga cggtttcgtt
     1381 gccgggaccg gactctccct cgacctccca ccaccatcac cctgaagcgc cgaaatcagt
     1441 atccgtcccc cggacacgta gcgcagagac gtcacccatc aggtgaaccg cacagcccct
     1501 acacaagggc gccgcctccc gatgacctcg gacccgcagg gtcacagcag acgacgcaca
     1561 cgctttgcac ccccaacacc tctgtccatg gcggcggcgg tagcccccgg cttccggtac
     1621 gtcgtcgacg tcaacctccg gaacccgcag ccgagcctgc ctgtggccga acccaagtgg
     1681 gtcaccgtca cggacatgga cctggtcaca taccggatgc cgttggtgat ctccactcga
     1741 gcccccgctc acccacagca agtgactgcc tgtcatcccc tgaggtgggt cacaaaattc
     1801 caactcggcg atgacccgct gcgtggcgtg cagcgtgtta cccgcgccgc aggcagcagg
     1861 cagcgcgccg acgggtcgag cggtacgacg cgtgccgggc cagttcgcgt ccgaccatgg
     1921 cggcgaacgc ctcccggccg tagcgggcgg ccctgggtac ggtggcgagc agcgcgtccg
     1981 ccgcccgcct gggactgccc ctgagcgcga ggaccgcgac ggcggtgagc ggggcggatg
     2041 gcgccagccg tctctcccat tgctccacgg gcccgggtgg ccgggtcctg gcgcgcaggt
     2101 gccgcgccgg ccgtgtgcgc ggtacgcact ccgggcccga gcacaggtcc ctctcgcgcc
     2161 gctcccacac ccgccgccgg gcgcggacct cgccgagcag cagcagccgg tgcgccgcgt
     2221 cgacggcggg catgccgatg ccggcgcaga gcccgttgac ggtggcaccg gccagcgaca
     2281 gcagcagatc ggtccgtacg gggccgacac gccgcatcag cggtcggcgc agctcccgct
     2341 cgagctccag cgcggcgacc gccgcgggca ccgcgcgtgg cagcagccgc aagacccgct
     2401 ggacgacccc ggcggcgacc gccgcgcagt cgaccagcaa ctcggccgcc acggccgcaa
     2461 cgggagcgtc gtcgcccgga gtgacggctg ggcccaggcg aactcctcgt cctgcacccc
     2521 ttgggcttct tccacggccc tgaccatgtc caggagccgg tcgaggccga ccctgcgttc
     2581 gtcgaacgag agcaggacct ggccggtgac cgcgttgacc tcggcccatc gcaccccgtt
     2641 cagcccccgc aggcgcgctg ttcgacctgg ccgtcgccga ggacaccacc ccgatggccg
     2701 acctggtcac ctggcggtcc cagttcggcc ctccggtcac cgtccgcccc gacaccgccc
     2761 tcggcgaggc cgccgaccag ctggtcgact cccgcgccac ctcacttctg gtggtggacg
     2821 actccgaccg gcccgtcggc cggatcctcg ccgacggcct gctcgactac ctgctgcccg
     2881 agcgcgggcg cctgcacttc cggaggttcc tgcggtgacc cgcgatctgg acaccacaac
     2941 ggccgcaccc gccgcacccg ccacgcggcg gcggggcctg ggccgtctca ccgtggtcgc
     3001 cgcgatcgcc ggtccgggcc tggtcgccgc gaacgccggc aacgacgccg ccggcatcgc
     3061 cacctacgcc tccgccgggt ctcaatacac ctacggcacg ctgttcttca cggtgctgct
     3121 cgccatcgcc ctggtcatgg tccaggagat ggccgtccgg ctcggtgccc acaccggcaa
     3181 gggcctgggc gcactgatcc gcgagcagtt cagcctgcgc ctgaccggcc tggcggtgtt
     3241 ctgcctgctg ctggccaaca cgggcctggt cgtcagcgag ttcgcgggca tcggcgccgc
     3301 cttcgaactg ctcggtgtgc ccaagtgggc agtgatcccg ccagcggcga tcctgctgtg
     3361 gtcgctagta ctgttcggct cctaccgatg ggccgaacgc atcttcctca tcatgtcgcc
     3421 ggcgttcttc gcctacccga tcgcgatgat cctcggccac ccgcactgag gcgaggtcgg
     3481 caagcacctg gtgatcccgc acatggaacc caacaagggc ttcatcctgc tcgcggtcgc
     3541 cctgatcggc acgaccgtca gcccgtacat gcagttctac gccgccgcag gtgtcgtaga
     3601 ccgcggcgcg aagcccgagg actagcggct gatcaagctg gacgccgtga tcggggcggt
     3661 gttcgcctgc atcatcagcc tgacgatcat catcgccacc gtcgccgcca ttggcgacac
     3721 cggtccgctg gactccgccg cccaggccgc cgaagccctc aaaccggtcg ccggccagaa
     3781 cgccgaaatc ctcttcgccg tgggtctgat cggcgccagc gccctggccg gcgcagtcgt
     3841 accgctgtcg gccagttacg cggtcggcga ggctgccgga gtggaacgct ccgtctcccg
     3901 cagcttccgc gacgccccac tgttcctggg cctgttcacc gcccagatcg tgctcggcgc
     3961 cgcggtggcg atgaccccgg tgaacgtcat ccagctcctg atcggcaccc aggtcctgca
     4021 gggcctgatc agccccaccg tgctggtcta cctgctggtc ctcaccaacc gccgttccgt
     4081 gctcggtgcg gcggccaacg gccctcgcta ccggatcggc gccaccatcg tggtcgtcgg
     4141 cgtcgcaggc atatccacca tcctgctcgt ccagaccgtc ctcggctggt tcggtctcgg
     4201 ctgatcccac cccgaggaac ccgccaagtc cggccgaccg gacccagatt gcggctacgg
     4261 atcggccggg aaaacattcc ggggccgccg cacaggggcc actcgggggc tgaccaggcg
     4321 cagactggag ctcgaggccg agtactggcg ccttctggcg gccggggtgg gcagcgtcga
     4381 ggcgtgcagg caactcggca tcgggcgcaa gactggctac cggtggcggg ctgagaacgg
     4441 tggcctgcaa ccggactatc tcccggaagc ctcgcgctcg ggtcggtact tgtcgctgct
     4501 gcagcgacgc ggtcgaggcg tccagtgcgt cgcacaagtc cgccactccc tcatccacgt
     4561 cccggttgcg cgccttgacg attccgtcgg tgaacagggc gagcacagta ccctcagcga
     4621 actcgaactc cgcgctctcg aagggcagcc caccgatgcc cagcgggggg cctatcggca
     4681 ggtcgagcac ttcagcctgt cgggcccctg ccccccggcc ggcagcgacc ggcgggaggt
     4741 ggcccgcgct ggccatggcg catgtacagg aggtggggtc gtatacggcg tagaggcagg
     4801 tcgctcccag cgccctgtcg gtcccctcgt cgcccggtgt gcggtgtacg ccgactcgtg
     4861 tctgacccac caagtcgtcg agtcgggcga ggagttcgtc cggtggaagg tccagggcgg
     4921 ccagtgcgcg cacgctggtg cgcaaccggc ccatggtggc tgcggcgtcc actccgtgcc
     4981 ccaccacgtc gccgaccacc agtccgaccc gggcgcccga cagcggaatc acgtcgtacc
     5041 agtcgccccc gactccgaga ggaccggcgg cggggatgta gcggtccgcg acctcgattc
     5101 cgctcgactg gggtagcgct cgcgggagga ggtcacgctg tagtttcagc gccgtggtgt
     5161 gctcgcggat gtagcggcga gcgttgtcca ggcacaccgc ggtgcgcgag gtcagttctt
     5221 cggcgagggc gcgatcctcc tcggtgaagg gtgcacgggg cggctgccgc aggagggtga
     5281 ccgtgccgag cgtagtgctg ccgaggagca gaggcagggc cagcaagcgt tggtgcgcag
     5341 tcgtcgagcg gatcccgatg gcaccgagca gtacatcact attcgtgatg tcggagttac
     5401 tcttcgtgcg ctgtgtcatg agccgtagcc ccgccggtgc gccagaagcg taggacggga
     5461 cttcctctcc tcctaggacc gactccgcga cctcgatccg tacggagtcg gcgaattccg
     5521 gaacgacgag ccccgcgagt tcctccgccg tctgcctggc gtcgagcgtg gtaccgatcc
     5581 cgctggcgcg gccgagcagg gccaggcgag tctgcgcttc gtagcggtcg gtgacatcga
     5641 cggccgcctc gcagatgcct agggggtggc ccgcctcgtc ctgcagacgg aagtaagagc
     5701 atgagtagac ctggtagtac tcggaggcag cgggggtggg cccgcgccaa tggacgtcga
     5761 gcaccggttc gccgctgctc aacacctcgt tcaggacctc gtccatgtcg tggtatcccg
     5821 gaggagagag gatcttgccg tgcggataga gcacaatgtg gggccgtccg aaccattcac
     5881 cggcgtgcca gcccatggcc tcctgcacag cctcattgac ccacttgagt cgggtatcgg
     5941 ggccgtagac gctcagcccc acgggcgcct gtgtgaagag ccgggacaac atcgactggt
     6001 ccgtcatccg ctcctcgtag cggtcgcttg ccaccgccat cacgacccag gcgggcctcc
     6061 cctggccctg ctccaccgga aagatgcgca agaccgtctc cgccagcgac ccgtccgggc
     6121 gtcgaacggt acgggtctcg gtccgggcgc ccccggatgc cggtgccccc ggtgcctcga
     6181 acccttgcag cgtgtcggcc cgggcccgaa cgtcccgcag cacctcgctc accggacgat
     6241 cgagcacgtc acgggtgtca gcaccgtaga gactgctggc tccaggactc cagccggtca
     6301 ccctcccccg cgcatccacc acgatcatcg ctgccgtagg cgtgtcgaaa gggctatgat
     6361 gaccggtctc acgtgatgcg cgcatcatgc acacgacctg ccaagtactg gaacgtctgg
     6421 aatcaaggct acgcctggct cccgcggcgc gtcttacgcc ccaggtcacc gccactgcga
     6481 tcccgggaca tcgcgcatcc ccccggctga gggagacagt gctcctgttc gcgaagtagt
     6541 gcgtcaccgc ccctgtcgtg cacccggcgc actgtgccac cttgcgcagc gacgcgttgg
     6601 cacacccctc cttcacgatc acccgaagcc gccgtcttca gtaactcggc tcacttcgcc
     6661 acatggtcac cggtcggagg ccacgccgca cgacagaccc tgtcagggca gttcagcgcg
     6721 catggcatgc gctatgacct ctccgatgag tacggctgtc gccgctgggc cgcgcagtgg
     6781 ggccgtgggc aggatcgagg tgtcggcgac gcggagaccg tgcacgccgt ggactcttcc
     6841 gtactggtcg accaccgcat tcgcggtgtc cgggcccatc ggtgcggtcc cgcatgtgtg
     6901 gtgagcggtg ccgatgttcc ggttgatcca tccgtccagt gcgtgatcgc agttgagcac
     6961 gtccggaccc ggttggagca gtccgtggga gatttcgtcg aaggccggtg tggtaatgag
     7021 ttccgcggtg gcgcgcacgg cttcgcgcag gcggcggcgg tccttggggc tggcgaggta
     7081 gccgtactcg atacgcgggg gcgtcgcggg atccgcggac ctcagggaca ggcggccggt
     7141 ccttccaggt gactggaccg agacaaggaa gggcaaaggc gcgccgggca cggtcgtcgt
     7201 gccgccgacg agtcctgcca gggggaccag cgactgcagg atctccaggt cacccggatg
     7261 ggttccgtcc gaggaggaca ggtgcaggca gccgcccagc caggagccgt gcggcgcgcc
     7321 cagttcccgc cgtggggacc actccaggac gagctgaggg tggtcgctga agcgggtgcc
     7381 gaccgccggg gcatccacgg cgacggggat gccgagccgc tccaggtcgg ctcgcgggcc
     7441 gatgccggac agctggagca gttgcggagt gacgagggag ccggcgcaca ggacgatctc
     7501 gccgccacag aagacggtct cttgcccgtt ttggcgtacg gcgactcccg tggcgcgacc
     7561 gttgcggatg acgatcctgc tgacggggca gtcgccgagc gcggtgagat tcgggcgccc
     7621 cagcgccgac agcgcatagg tgaggccggt gttgagccgc acaccgtcga cggcgttgca
     7681 cgggacgggg ccgaagcccg gcggcgcctg gtcgttcttg tcgggttcga gcgggtggcc
     7741 gaggtggtcc gccgcgtcgc ggaaggcaac tgcggcaggg tgggcgaggg atgcgcggcg
     7801 taccagcacc ggtccacagc cgccgtgcag gtcgtcggcg ccgtggtcga ggtccgtctc
     7861 cagggcgcgc agccaaggga gcatccggtc gtacgtccag gccgggttgc ccgctgccgc
     7921 ccagcggtcg aagtctgcgc gccgggcgcg gacgaaatag ccgccgttga cggtggtcga
     7981 gccaccgagg tggcgcccgc ggaccaccgt gtagggccgc tcgggcgtga ggtgcgcggc
     8041 gtaccgctgg acggcccggt ggtccggctg ggctccggga atcagccgcg cgtcgagcag
     8101 ctcgagcgcg aaatcgcgga gacggcgcgg aaacggccct gcttcgacga ggagtacggt
     8161 gcgtccggcg tcctcgctca agcgggccgc cagcgcggca ccggaagccc ctgctccgac
     8221 cacgatcaca tccgcggacg tccggtccgt ggtctcagcc accagtacgt cccgacggag
     8281 gcgtgacgcc atcggcggag agccaccaca gtctcggacg gagcggctcg gtggtgcgct
     8341 cccggatgca gcccttccag acaccgaggc tgtaggccag gtcgtcggcg aggcgggtga
     8401 ccgtccaggg caggaccgga agtccggggc ggctccgtcg gtgttccagg agcgggggca
     8461 gaagggcggc tgcggccagc gggagcctca gccggggtac ggctgcggcc agcggcaggg
     8521 ccagtggcca ccaggtgcgc gtcacggcac ggccgagttg ttcgcctgcc agcaacgtgc
     8581 cgcctccgct cagccgggcc gcggtggccc acggtcgatc cgaccaggcc gcgagtctgc
     8641 gcgccagcag tccggtcgtt cccgccgtga ccagggcacc tgcgagcggc cgtcgtgcca
     8701 gggcgagtcc ccaggcgagc gcgctccagc cggccatcga cgtggccggg agcgtgcccg
     8761 ggtgccgggc ggccagcggt gcggcggagg tgccgtactg gatgcgctgg gccagccact
     8821 ggtgcggacg cgtccggtgc cggtgggcca cggtggcgga ggggtcgtag cggacgtggt
     8881 gaccgtcggc cgcgagccgc cacaccaggt caacgtcctc cccgacgcgc atcgtctcgt
     8941 cgaagccccg gcccacggcg gtgcgccgca ccaccaacgc ggcgctgggc acatagggca
     9001 cgtgcgagcc gggtcgtacg agtgactccc tgggccccat gtccaaggcc gagcgcgcac
     9061 attcgtaggc cgcgaggaag ccacgtcccg ctccctctgc cacgatgcgc ggcgccaccg
     9121 cggccacggc ggggtccgcg aagtgcggca gcaaggcgtc ggcccatgcg cgggtcggca
     9181 ggcagtcgga atccacgaag gccaccaatt cggtggtggc gtgggcgagt ccggtgttcc
     9241 gcgcggccgc cgggcccccg ttcacagggc gacggaccag cctggcgccg gccgcccgca
     9301 cgacgtccgc aaccgcgccg gggtcggccg aggcgtcgtc cacgacgatc acctccggtc
     9361 cgccgctcag gccggccaga cagcgggcga ggtgcccggc ccggtcccgg accggtacca
     9421 cgaccgtcac ggacgggcgc ggtgcctcgg acggcggtac ggggtgcatc atgcctgcct
     9481 ggaccaatcg acgggccagc agtccggtcg ctcgcccttc cccgaccggc ttcccgtcga
     9541 gccacgcgct cacgactggc acggcgcgcg ccgtcagccg catcagccgg tacggggagc
     9601 ctcccaacag gacccgcccg tcccgcagca gacgcagtcc ggggtcgggg cgcagccgga
     9661 agaccgcggg gagtggggga acgtcgatca ccaggtgtcc tcgaacatcg aaaggccggg
     9721 cgaccagcct tcacccgctc gtctcgacgc gtcgatcccc caggtgggca gcaagtgcgg
     9781 cgcaccgacc cgctcacggc accggggata cctaccgtca ccctgcgccg cgacaccggc
     9841 cgcgccgtgg acaacgaagg gaacctccat gaccgacaac tcacagacct cgaccgctga
     9901 ccgtctcccc gccgaggacg accaactgat cgaggaggac ctgcttgtcg aggacgtgtc
     9961 gatcgacggc atgtgcggcg tgtactgagg cggggacacc atgcccacgc acaggaacgg
    10021 gtccgagcgg ttcgacctgg aggcggcctg ggccgtgcac ccccaggtcg ccgttcgtcc
    10081 ggagcccttc ggggcgctgc tctaccactt cggcacccgg caactgtcct tcctcaagga
    10141 ccgccggctg ctcaccgtcg tacaggaact ggcccaccgc ccgagtgcgg cggaagcctg
    10201 ccgggtggca ggcgtcgacc ccgccgagtc ggggcgctac cgcgacgcgc tggcgcagct
    10261 tgcgaagtcc cggatgatca ccgagcgcac cggtccgccg agccgcggtg acgctcatct
    10321 gcaaggagag gacgcgcgat gaccgtgcct tccgtagcac cgccgagccc ggcccggacg
    10381 ggccgcctcg tggaccattt tcagcacggc ctcgaagcgc cgatctgtct gacctgggag
    10441 ctcacctacg cctgcaacct ggcctgcgtg cactgcctgt ccagctccgg tcggcgcgac
    10501 ccgcgcgagc tgaccaccga cgagtgcaag atggtcatcg acgagctgga gcgcatgcag
    10561 gtcttctatg tcaacatcgg cggcggcgag ccgaccgtgc gcccggactt ctgggaactg
    10621 gtcgactacg ccaccgccca ccaggtgggc gtcaagttct ccaccaacgg cgtcaggatc
    10681 accccggacg ccgcgcggcg gctggctgcc agtgactatg tggacgtcca gttgtccctg
    10741 gacggggcga cggccgaggt caacgacgcc gtacggggcg agggttcgta cgcgacggtg
    10801 ctgcaggcca tggagaacct tgccgcggcg ggcttcgccg ggttcaagat ctcggtcgtg
    10861 gccacccgcc acaacatcgg gcaactggac gcgttcaagg cactcgccga ccggtacggg
    10921 gcacagttgc ggctgacgcg gctgcggccc tccggccggg gcgccgacgt gtgggacgaa
    10981 ctgcaccccg cagcagcgca gcaacggcag ctctacgact ggttggtcga gcacggcgac
    11041 gaggtgctca ccggggactc gttcttccat ctctccgcct acggcgaggc gttgcccggg
    11101 ctgaacctgt gcggcgccgg gcgggtggtg tgcctgatcg acccggtcgg tgaggtgtac
    11161 gcctgcccgt tcgccatcca cgacgccttc cgcgccggaa acgtccgcga cgagggcggg
    11221 ttcacccggg tatggcgctc ctcgtcgctc ttccaggagc tgcggcagcc tcagacgggt
    11281 ggcgcctgca cgaagtgttc cgccttcgac gcctgccgtg gtgggtgcat ggccgccaag
    11341 ttcttcacag gactaccgct cgacggcccc gatccggaat gcgtccaggg acacgcggag
    11401 gcggcgctgg ccggcgaccg gtcgtccgct ccccggccca gcgaggacca ctcccaccgg
    11461 gggccggtgc gcgggcagcc cgtccttctc ggcctgccgg ttcggcggga gagccggccc
    11521 acgcgcatgt gcgacgaaag tcctctggcc tgaccgaaag gccgctcggt ccacgaaaga
    11581 aaggggcgtc atgcccaacg catggttcga aaccgtcgcc gaggcgcagc gtcgagcaaa
    11641 gcgacgactg ccggcatccg tctacggcgc cctgctcgcc ggttccgagc gcggcacgac
    11701 ctaccaggac aacatggccg ccttcgccga ggccggcttc gcgccgcacg tcgcggatct
    11761 gccggccaag cgtgaccagg ccacgaccgt cctgggccag tcgatctccc ttccggtgat
    11821 catctccccg accggagtgc aggccgtgca ccccaatggg gaggtggccg tggcccgcgc
    11881 cgcggcagcc cgaggcacgg cgatgggact gagctccttt gccagcaagc ccgtcgagga
    11941 ggtcgtcgcg gccaatccgc agacgttctt ccagatgtac tggatgggcg gcaaggactc
    12001 catgatcgcc caggtggagc gtgcccggga ggccggcgcc aagggcctga tcgtgaccct
    12061 ggactggtcg ttctcgcacg gccgggactg gggcagcccc cacatccccg agcgcctcga
    12121 cctcaaggca atggcgcgct tcgcgccgga gggaatcacc cacccgcgct ggctgctcgc
    12181 gttcgcccgg acccggcggc tgcccgacct gaccaccccg aacgtcgccg ccgtcggcga
    12241 gcaggccccg acgttcttcg gcgcctacgg cgaatggatg cagacacccc ctcccacctg
    12301 ggacgacgtg cggtggatgc gcgagcagtg gggcggcccg ttcctcgtca agggcatcat
    12361 ccgcgtcgac gacgccaagc gcgccgtgga cgccggcgcc caggccgtct cggtctccaa
    12421 ccacggcggc aacaaccttg acagcacccc tgcgaccatc cgtgccctgc ccgccatcgc
    12481 cgacgccgtg ggcggccaaa tcgaagtact cctcgacggc ggagtgcgtc ggggcagcga
    12541 cgtggtcaag gccctggcac tcggcgctcg cgccgtgatg atcgggcggg cgtacctgtg
    12601 gggcctggcc gcgggtggcc aggccggcgt ggagaacgtg ctcgacatcc tgcgcggcgg
    12661 catcgactcc gcgctcctcg gactcgggcg ctcctccatc cacgacctca cacccgacga
    12721 cgtggtgctc cccgacggct tcgcccgcgt cctcggggca ccgcgcccgg cccaggcccc
    12781 ctgatgacca cgctggcctc cctgacctgg accgacgtag cagatctggc ggggcggggg
    12841 accgtcctcg ccgtgccggt cggcgccacg gagcagcacg ggccgcacct accgatgtcc
    12901 acggacaccg acatcgccgt cgccctgacc gaccggctgg cggcacaggc gcccgatgtc
    12961 gtcgtggcgc ccgccgtggc gttcggatcc agcggcgagc accaggattt ccccggcacg
    13021 ctgtccgtcg gccaggaggc cgtcgagctg ctcctcgtcg agctcgtgag gtcggcaacg
    13081 gtgacattcc ctcgcgtcgt cctggtctcc gctcacggcg gcaacgcaca gcccgtcacc
    13141 cgggccgtcc gacgactggg taccgagggg cggcacgtcc tggcctgggg cccgcgctgg
    13201 ggcggcgacg cccacgcggg ccgcaccgag acctccgtca tgctggcgct ggccccccac
    13261 ctggtacgga tggaggcagc gcgagccgga aacaccgccc ccgtccacac actcctcccg
    13321 cgtctgcggg acgccggcct gtccgccgtc accgccaacg gcgtgctcgg cgatcccgga
    13381 ggcgcgaacc ccgacgaggg ccggctgctc atccagcgag ccgtggccga gctcgcggac
    13441 ctggtgaccc ggcatcgccg gcacaccgac gccgcctctt gagcaggcgt tccgcctgtc
    13501 cggacttgag cgcaggcgct gccacacagc cggtgggtga acgcctgcgc tgcgactcga
    13561 aactcagccg acgagtccga gctcctgagc gcggctggcc gcctgggccc ggttccgcac
    13621 ccccagcttc cggtacaccg tggtcgtgtg ctgtttcacc gtatgccggg acagattcag
    13681 cacagctgcc acttccgggt tactcaaccc cagcaccaga tgctgcagga cgtccagttc
    13741 acgcttcgaa agccgcgccg cggcgggtgc ctcatccttc gggaacaccc gcgcgccctc
    13801 cgcgacacgc ttcaccgccg ccacgatcgc cgcagtcggc atctgcttcg acaaggacgc
    13861 caccgcgccg tgcgccgacg ccagcgccgc cgacacccgc ccctctccgg acagcagaac
    13921 aaccttgacg tgcggcatcc ggtcccgaag gtcccggcac aggtccagcc ccgaccggcc
    13981 tcccaacgac gtactcacca acaccagctg cggatgatga cgccgcgcca cctgccaggc
    14041 ggcctcctgc gaatccgccg ccagacacga cgccacccac ggctggctca gcaacaccgc
    14101 acgcaacccc gccagcacca gctcgtgctc ctccgccacc agcacccgct gcatctgaca
    14161 tcggtcctcc agcaccgggc gaacccgacc cgtgggagca acccgctcgg cgaggacaag
    14221 tgtcatgacg acacctccga ctactcctag aacatgcaag aacggaccgg ccggccaacc
    14281 gcggcaggag ggctcgccag gcgcattgac cggagaggct gaagaaattt cacggatttc
    14341 ccgatcctcc gacacgtccg gctctccccg tcgtgacctg cggtttcacc ggcgcgatct
    14401 gacctcacag cttgaccacc gaccggcgga cagacgcccg gggaactacc cgtacgagta
    14461 cctgtccccg ggctcccggg ggtctcggct cgggctgtgt acggcggtac gatcggatca
    14521 ccgctcccga actgcgcacg tggggactcg atgcctactc cgaacgccgt gccggcggaa
    14581 ctcaccagct tcgtggggcg gcgaaaggat gtcgccgcgc tccggcagct cttctccacg
    14641 acccggctgg tgacgctgac cggcatcggc ggggtcggca agacgcggct cgctctccgc
    14701 gtcgccggag agatgcagcg agccttcccg gacggtgtct gtctggtgga actggcgtcg
    14761 ttgaaagacc cggcgctgct cccccacagc gtggtcgacg ctctcgggat caaggagcag
    14821 tccccccgct cacccatgac cgtgctgcgt gatcatctgg ggcagcggcg catgctgctg
    14881 gtcctggaca actgtgagca cctggtggac gcggccgcgg gcctggcaga ccaggtgctg
    14941 cgtgcggcgc ccgaggtgcg gatcctggcc accagcaggc aggcgctcag aatcgccggg
    15001 gagcacatct accccgtccc accgctgccg actccccctc ctgatgagtc cgtcgagccc
    15061 ggcacggcga cccagtaccc atcggtcact ctgttcgccg accgttcggc ggccgtcgtt
    15121 cccggcttct cgatcacgcc cgacaacgaa gcggcggtcg tccgcctctg ccggcgcctg
    15181 gaagggatcc cgctggccat cgaactggcc tcggtgcggt tgcgggtgct caccgtccat
    15241 gatctcgcga gccgcttgga cgaccggttc cagctcttgc gggagggcac ccgcaacctg
    15301 ccggaacggc atcagaccct tcaggcgctc atcgactgga gctatgacct gtgcaccccg
    15361 aacgagcaga ggctgtgggc gcgggcgtcc gtcttcgtcg gtgggttcag caccgacgcg
    15421 ctcgaggcgg tgtgcaccga tgaggcgctc ccccggcatg cggttctcga caccgtcgcc
    15481 ggcctcgtcg acaagtcgat cttcattcgc gaggagcagg gagagtacgt ccgcttccgc
    15541 atgctggaaa ccatccgtgc gtacggccag gcccggctct cggagtcggg cctggaaccg
    15601 gccctccgcc ggcggcaccg cgaccggtac cggcaactca tggaggtggc gggggacgag
    15661 tggagcggcc cgcggcagcg ggagtgggcg agcaggcttc agttggagct cccgaacctg
    15721 cggcacgcac tggaatactg cctgtcccag cccggtgagg cgcgtaccgg actgcgcatg
    15781 gcggcggcgc cctggctatg gctggcgatg gggtacccca ccgaggccag gctgtggctg
    15841 ggccgagcgt tggcgctcga cgcggagccc agccgcgaac gggcctgggc gcttgccacc
    15901 gtggcgtaca tcgccgcatg ccagggagac gaggctgcgg cagcggagtc ggccgaggac
    15961 gcccgaaagg tcgccgtcca cctgaacgac cccgccgcgc tcgcctatgc cacgcacgtg
    16021 cgcggactga gcaagtttct cagcacggac ctgtccggcg cgatccccct gttcaacgag
    16081 gccctcgagc agtacgccga gaccaaaaca cccgtccact atccgaacaa cctccggacg
    16141 gagttggcag cggccctcat cctcctgggc gaactcgaca aagccgcgga gatcatcgag
    16201 gagttgttcc ggcgctgcac agcggccggc gatcagtggg tgctgtcatg ggtgctgtgg
    16261 gggcgcggat tcctgcgctt gattcacggc gagctcgacg cggcggaagc cgacttgtgc
    16321 gaggcgctca ggatcaagag gttcttccac gacacccttg ggatggcgct cgcactcgac
    16381 gtactcgcct ggaccacggc ggcgaagggc aacgccgagc gagccgccac gttgttcggc
    16441 ggggcgaacc ccctgtggca gaccctcgga gccccgctgt tcggctggac gctcctgctc
    16501 gcacagcgag ccaagttcga gacgatggcg cgggacaaga tgggggacgc ccagttcgac
    16561 gcggctctcg aacgtggcag tgccctgacc accgaggaaa cgctcgccct cgccctgcga
    16621 gagcgcacac agcccgcaag cgatgcgcca cgtcctgctt cgacgctgac ccggcgtgaa
    16681 cgagaggtgg ccgaactggt ggcgcagggc ctgtccaaca aggacatcgc ggcacagctg
    16741 gtcatttcgc tgcggaccgc ggaagggcat gtcgagaaga tcctcaccaa gctcggcttc
    16801 aacacgcgta cccagatcgc gagctgggtc gtgcagagac agtccgagcg gacgtgaacc
    16861 accctcgtga tgagggcggg gcagacatcg gacgaggcag gtaccggaac aggtagttcc
    16921 acgctcaatg gcgcgatgcc cgaccggcag attggaggca gttccaccca agatcaccgg
    16981 agacgatgat catgtacgtc acgccccccc atgacagtga ccctggcgcc atggcgcctt
    17041 ccgggatcgc aggcgcacgc ccgtccctcg gacgcgaagc ccgccagtgg gacctgccgc
    17101 acgccccgca ggccgcgggc atcgcgcgcc acctggccct cgacacgctg aggggctggg
    17161 gagtccggga agaccaagcc caccaggtcg ttctcgctgt ctccgaactg gtcaccaacg
    17221 ccgtcgagca tgccctgccc ccggtcgccc tgcagctggt accgcccaac gagcaccaaa
    17281 cgatccacgt cgcggtcacg gacggtggcc cggcgaacga ccgcggtgcg cgaaccgccg
    17341 actgcgaacc cgacgagcac gggcgcggaa gcgccatcat cggctttctc gcctccgccc
    17401 gcggtacgcg catgtgcggc agaagcgcca cccactgggc ggacctgccc ctcaccgcct
    17461 agcccgtcgg agggacatcc cccacctggg cgggtttcgg ccagccgcac catggggtgg
    17521 cctgaaactc gccgtacgtt cgcccggctt cggctggttc ctctgaccgc gaaggcggcg
    17581 aacgaccatg ggagacgggc atgactgaat tcaccgagtc ggttgacata cccagagcag
    17641 atgcaacgag cgtcacgccg cggcgtggcg cactgatagc cattgcggcg ctcctggcca
    17701 ctgtgaccaa tgcggtcgcc ttcatgctcc cgccgctcct gccgctgatg cagcagcagt
    17761 tcgggctcgg ggtggcggcg tcggcctgga tcttcaccgc gctcacgttg ggcggcggag
    17821 caggcttcgt cctccttcca cgtctcaccg acgtgctcaa cgatcggacg acctcgctgt
    17881 tgtccggtgg cttcctggtc gtcggtgcgc tcgtgcctgc catcggtgat tcctacccgg
    17941 cgctcctggt cgggtccgca ctcctcgggt tcggtggcgc cgcacagctg ctgccactga
    18001 gcttcctgcg ccggcacctg accggtagtg cggtcgcaac ggctgtcagc gtgctgatca
    18061 tggctacggg ctccgggacg gtcctcggga tggtcggtgg cggactgacg gtcaagtact
    18121 tgtcgctcgc gagcttcttc tacattctgg ccgcggcatt cgcggtgacc acggtggcgc
    18181 tgctcatcat cgtcccctcg tcccggccgg agtcgtccgg acgtatcggc atcctcggaa
    18241 ccgtgtggct gatcgcctgg gtcacggctg tgctgctcgc cttgacccag ggcctcacgt
    18301 ggagcggcac ggcgatcctc gcggtcctgg gcgcgggtgt agtcggcggc gtcgtgtggg
    18361 gactggtcca gcgcaagtcc cccacggcgg tgttcgacat ccagctgctg aagaagcccc
    18421 tggtcacacc ggcatgtttc gccgctggaa tcttcggcgc catcgatgcc gcgttcctgc
    18481 tgctggtgac ctactacacc cagactcccg ctggggtcgg atacggactg ggtgtcgacg
    18541 ctctgggcac cggcctgctc atgctgccgt tcgcgctgat gatgttcgtc ggtggcaagg
    18601 ccgccgaaag ggcagtccag aagggccgtc cggccaccgt cctcgtcatc ggcaccgcgg
    18661 tctccgtcgt ggggctcggc tggctcgcct tcgcccacga ccagaggtgg cagtacctcg
    18721 tcggtgccgc cttcgtcggc ctcggcagcc gagtgggcta cagcggcgcc ttcgccgtgc
    18781 cgcagctggt cgtccccgag gacaaggccg ggatggccgc gggcatggcg ggcaccacca
    18841 tggccatcgg cttcgcgttc ggatcggcag tggtcaccac gctgctcacc ttcgacacca
    18901 tccccaagat cggcctgccg aaggagtacc tctacacggt tggctacgag gtcaccgtcg
    18961 ggctctcgct gctggtcctg gtcgtcacgc tcatctcgcg gctgcgccgc ggcggcgagc
    19021 tcacggcaag ctccgtggaa gcgtagcgag ccggcctggc gggagctcgt gcaccacacg
    19081 ccgcgcgttc ggagtcagcc ggccgcagtc tggtccgctc cgaacgcgcg gcgtgtggcc
    19141 gtccggccgc ggtgcacacc ccccactcgg gcaaggtctt cgcccggccg cacccaacag
    19201 ccccatatgc cacctacgtt gcgcgaaatg ccaatggagg tggagcgtgc ctgtacccgg
    19261 caatgcagca aagcgtcaca tcatcgcagt caacggctct gacgtgtggc ggagcgaatt
    19321 ccgcctgcga ttcggcgatt tcgacggcct cggccatctc acggcgtcgg tctacctcgc
    19381 ctttttcgag gagacccgct cggcctggat gaccggaacg ctcggcgtcg cgtatccgac
    19441 ctatgtggtg gcgacccagc acatcgagta cctgcacgag gtcacgcccg acgatggagc
    19501 cgtgagcgtc gaactggccc tgcgtgaggt gcggacctcc agcttccgtg tggtggagca
    19561 cttgacgacg gcaggctacc tctgtgctcg ttcgacagcg acgctcgcga tgtgggacat
    19621 ggagcgccgc cagtctcggc cgatcacccc ggacgagcgc gccgtgttcg aggcatacct
    19681 ggcagagccg acgcacccga tgacgccctg acgtgctgcg caacgcaccg actcaccccg
    19741 ccatccccca gccgggcaag gttccgagcg ccctcgctca tgtcatcagc tcccgtcgta
    19801 cgttcgccgc accacaagca agcgcttagt caatcaggga gtgcccatgc ccgaggccgt
    19861 catcgtcgcc acagcccgct cgccgatcgg ccgggccggc aaggggtcgc tccgggagat
    19921 gcgtcccgac gatctgaccg tgcagatggt gcgggcggca ctggccaagg tgccgcagct
    19981 cgaccccacc gagatcgacg acctggtact ggggtgcggc cagcccggtg gcgagtccgg
    20041 tttcaacatg gcccggatcg tcgcggtgct ggccggtctg gactcggtgc ccggaaccac
    20101 ggtcaaccgc tactgctcgt ccagcctgca gagcactcgg atggcgatgc acgcgatcaa
    20161 ggccggtgag ggcgacgtct tcatatccgc cggggtcgag acggtcagcc ggttcttcaa
    20221 gggcaacgcc gacggtctgc cggacaccga gaacccggtc ttcgccgagg cgcaggcgcg
    20281 cacggagaag ctcgcctccg gtggaggaac ctgggtcgat ccgcgcaccg agggcgctct
    20341 gcccgacgtc tacatcgcga tggggcagac cgcggagaac gtcgcccagt ccacgggggt
    20401 gacgcgcgag gaccaggacg ccttcgcggt gcgttcgcag aatctggcgg agcaggccat
    20461 cgccgacggc ttctgggcgc gtgacatcac gccggtgacc ctgcccgacg gcagtcagat
    20521 cgtccgggac gacggcccgc gtcccggcgt cactctggag gcggtctccg cgctcgagcc
    20581 ggtcttccgc cctgacggca ccgtcaccgc gggcaactgc tgctctctca acgacggcgc
    20641 cgcggcactc gtgatcatgt ccgacaccaa ggcgaagcag ctcggcatca cgccgctggc
    20701 ccgcatcgtc agtacggggg tcaccggtct gtccccggag atcatgggcc tcgggccggt
    20761 ggaggcgtcg aagcaggccc tgcgccgggc cggtctcacc atcgacgaca tcgacctagt
    20821 cgagatcaac gaggcgttcg ccgcccaggt gattccgtcg gcccgggagc tgggcatcga
    20881 cttcgaccgg ctcaacgtca acggcggcgc gatcgccgtg ggccaccctt tcggcatgac
    20941 cggcgcccgc atcaccagca ccctgatcaa ctccctgcag taccacgaca agcagttcgg
    21001 cctggagacc atgtgcgtcg gcggcggtca gggcatggcc atggtgctgg agcggttgtc
    21061 gtgatcaggc gcttcgaagg caggtcgtca cgcgtgcact cgactcgttc ggcagcgccg
    21121 acctgctcgt caacaacacc ggaatcaacc cggtgtacgg cccgctcatg acgctggatc
    21181 tcgacgtcgc ccgcaagatc gtcgaggtca actgcctcgc cgcgctgtcg tggacccagc
    21241 agatgtaccg ggcgtggatg tccgcgcacg gcggcgccgt cgtcaacgtc tcctcggtcg
    21301 ccgccgtccg ccccgcgccc ggcatcggcc tgtacggcgc cagcaaggcg atgctcaccc
    21361 acctcaccga agaactcgct gtcgaactgg gcccggacat ccgggtcaac gcggtggccc
    21421 cggccgtagt caagaccaag ttcgccaccg cgctctacga cggccgtgag gacgaggccg
    21481 ccgcggccta cccgctcaag cggctcggtg tcccggagga catcggcggc acggtggcct
    21541 tcctgctctc cgacgacgcc gcctggatca ccggccaggt cctggtcgtc gacggcggtg
    21601 tgaccctcac cggcgccggc tgatggcgcc cacccttctt cccacaggag cgaactgatg
    21661 accaccaccc gtaccgccat cgtcaccggc gcggcccgag gcatcggggc cgacgtcgcc
    21721 caacggctgg ccgccgacgg cctcgccgtg gcggtcgtcg acctcgacga gagcgcctgc
    21781 aagccggtcg tcgagcggat cgaggccgcc ggaggccggg cgctggcggt cggcgtcgac
    21841 gtgagcgacg agccgggcgt acagaccgcc gtgcagcgga tcgccgagga actgggcgat
    21901 ccgaccgtgc tcgtcaacaa cgccggcatc atccgggaca acctgctgtt caagatgacc
    21961 gccaccgact gggacgcggt gatgaacgtg cacctgcgcg gatcgttcct gatgtcccgc
    22021 ggggtgcaga gttacatgac gaaggccggc tggggccgca tcgtgaacct ctccagcacg
    22081 tcggcgctcg gcaacaaggg ccaggccaac tacgccgcgg ccaaggccgg gctgcagggt
    22141 ttcaccaaga cgctcgcgct ggagctgggg aagttcggcg tgaccgccaa cgcgatcgca
    22201 cccggattca tcgagacgga gatgaccgcc gccaccgcgc agcggatggg catgtccttc
    22261 gacgacttca ggaaggccgt gacggcgcag atcccggtgg cgcgcagcgg tctgccgaag
    22321 gatatcgcgc acgccgtgtc gttcttcgtc agcgaggacg caggatacgt ctccggccag
    22381 gtgctctacg tcgccggagg gccgcaggcg tgaaggtctt ccggggcttc gaagaactgc
    22441 ggaacgccgt cggcacccac ctcgggtact cggactggca caccgtcacc cagcagcaga
    22501 tcgacacctt cgccgacgcc accggcgacc accagtggat ccacgtggac gtggagaagg
    22561 cggcggccgg acccttcgcc ggcaccatcg cgcacggcta tctgacgctg tcgctggtgc
    22621 cgatgctggt ctggcaggtc taccgggtcg agggggtgtc gatgggcgtc aactacggcg
    22681 cgaacaaggt ccgcttcccg tctcccgtgc cggtcgactc caaggtgcgc gccggcgtcg
    22741 agctcctgtc ggtggaacct ggcggcggtg ggtaccgcgt cacg
//
