LOCUS       NZ_WHOM01000078        10909 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces apocyni strain TRM 66233 522, whole genome shotgun
            sequence.
ACCESSION   NZ_WHOM01000078
VERSION     NZ_WHOM01000078.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces apocyni
  ORGANISM  Streptomyces apocyni
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 662149)
  AUTHORS   Liu,W.
  TITLE     Streptomyces poacynumensis sp. nov., a endogenous actinomycete
            isolated from Poacynum hendersonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 662149)
  AUTHORS   Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Key Laboratory of Protection and Utilization
            of Biological Resources in Tarim Basin of Xinjiang Production &
            Construction Corps, Tarim University, Xingfu Road, Alar, Xinjiang
            843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009739465.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,397
            CDSs (total)                      :: 6,319
            Genes (coding)                    :: 6,205
            CDSs (with protein)               :: 6,205
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 34 of 114
            Pseudo Genes (incomplete)         :: 100 of 114
            Pseudo Genes (internal stop)      :: 6 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:56:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 407990
            Orig. end                         :: 418899
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHOM01000078.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10909
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="4"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10909
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10909
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5909](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5909)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            63..2165
                     /locus_tag="GCN81_RS30475"
     CDS             63..2165
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020126815.1"
                     /locus_tag="GCN81_RS30475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AAA family ATPase"
                     /protein_id="WP_156727765.1"
                     /transl_table=11
                     /translation="MTDEMDLGAAAAEFDRTTAAEAEKAAEDERKQVLSQFPLEQWVTL
                     PLSRYALGQTAQSGSGPTYCRLIEFGTPNLGSIKGGSAAKHIMYHHNSGEWRIAAPLRG
                     MEPQESWEELRGQFVRAFEAVAAGDFEAVNDLEALRYGQTLVTKSLATYFPEHFLPIYA
                     GEHLRKFVTLLDGAAESDAPAWDSNRRLLELVRSRAEFDGWSGQEVMHFLYERFDPRPQ
                     QRTIWKIAPGERGRLWDACRDGGVIRVGWDELGDFGQYQSDTELKQALDAHWPRSSGGS
                     LTLARRLLAFRDLEAGDRIVANRGMDEVLATGTVNGSYRFDADHSEYRHVVPVSWDVSH
                     ARKLSKPQHGWRSTFAKVQPALMAWITAGLAESDTEPAGTHAGSPGTLPEDVAGVLEAL
                     ERKGQVILHGPPGTGKTRLALGTALALAGRADALNSTSARRSTAQAEMLRNGRIRMVTF
                     HPSYGYEDFVEGFKPDLSATGPGLTLALTDGVFHTLCAQAAARPDETFLLIIDEINRGD
                     LPRIFGELVTLLELDKRGLPLALSVSRRTFSVPSNVRIIGTMNTADRSISHLDAAVRRR
                     FAFLPLGPDPDAVSGTVGPLELAEFFESLNTRIARHLDADHQIGHAYLLRNGEPIATED
                     DLAAAFHHEVIPLLEDYCLGRADLLHRILGGLVDADTGRPALMSPQDLAAALATEFTSG
                     TPGPDA"
     gene            2158..3414
                     /locus_tag="GCN81_RS30480"
     CDS             2158..3414
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020126816.1"
                     /locus_tag="GCN81_RS30480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="McrC family protein"
                     /protein_id="WP_156727766.1"
                     /transl_table=11
                     /translation="MPDRTEVRLAEYGSALLGAEQLTPRDVERLRGLHTRGRLSLDQAR
                     SGWRLRADATVGILVLDRIRLVIEPKFAIPGEQLMRWLAYALGAPAPLPATARRWATGP
                     DGYADLVAAALLEQCEQLVREGLRRDYVRRQSLEPVLRGRLDVAAQVSRRYGQLDQLHV
                     RAFDQEADIWDNRVLGTALRVARALATRPDLVRDLHAIADAFPQAPTPDAALRTLDRTR
                     YTRLNARYRTAHTWARLLLSGGGVTDLLTDDGMTADGLLLAMPALWEAVVRRLATEAAA
                     PHGGHTVPSSGATGITVRGDLGSTSTFRPDVLLSLPGRAPSARTLLPVDAKYKRYDRYG
                     VGAGDVHQLLTYSAGYTPNTDLRAVIVHPCPGGHSHRVLQVSGPRGSLGSIHVLGVDTL
                     AAPEQAAVWIRSVAPWGDW"
     misc_feature    3970..3985
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.24"
                     /tool="antismash"
     gene            3987..4571
                     /locus_tag="GCN81_RS30485"
     CDS             3987..4571
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513779.1"
                     /locus_tag="GCN81_RS30485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4352 domain-containing protein"
                     /protein_id="WP_156727767.1"
                     /transl_table=11
                     /translation="MRHTRTSAIAAAGLLALTACGTGEETVNTTPKKTVTKTVEGAADS
                     TASSTPEAEKKAAAVGDTLTLRGFEDGEQLDVNLKRWLPTAKPADEYAVPQDGKRWAAA
                     QFELVNTGSKVYSDSPSNGAKAADAQGQRFDSWFGEITAGPEMASQVTLPRGEKALGWV
                     VFEVPKGSKIVSVQFGMNSGFSDESGQWSVS"
     gene            complement(5001..5909)
                     /locus_tag="GCN81_RS30490"
     CDS             complement(5001..5909)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894803.1"
                     /locus_tag="GCN81_RS30490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_197287859.1"
                     /sec_met_domain="AfsA (E-value: 4.8e-23, bitscore: 76.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPSTGPSPTCIPQQYVHKFNEAEVLLTGWQPTGPDTHALTAAWP
                     HDHPFYRSAHGLHDPLLLAESVRQAIPMLCHSAYDVPFGHRQIWQHFRFRLGQEAVADQ
                     GRPADLQLRIACGNIERRRARLSAMTMGIDLTGDGLLLGTAHVGFTNHPFALYRRMRAG
                     RADPAGTARTALSPGEPVVPELVGRTRQRDVVLTSTDSEGRFLLRVDLGHPTLFDHPVD
                     HVPGMLLLEAARQAANAVAHPRRTVLTAMDATFVRYVEFDSACSVEACVRPDDTTGALG
                     VRVRAEQNGTSVFTASLKLRT"
     gene            6069..6707
                     /locus_tag="GCN81_RS30495"
     CDS             6069..6707
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891475.1"
                     /locus_tag="GCN81_RS30495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_156727769.1"
                     /transl_table=11
                     /translation="MRTVAESKQERAVRTRRQVLVSAAELFDAYGFAGTSISRICERAE
                     TTAGALYHHFGSKEGLAKAIMNAQPDTILPWLESEGLQRLVDITLIWAHQLQTDPLLRA
                     GVRLTGEQATFGVQDASPYEDWTRIMEECLGVAAEKGELQADVTPRNVAEFVVEACTGV
                     QTVAAVLTGRSDLAARAVRMWQFLLPGIAAKDVVVRIDLDPARVQVEPV"
     gene            6767..7489
                     /locus_tag="GCN81_RS30500"
     CDS             6767..7489
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953814.1"
                     /locus_tag="GCN81_RS30500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAD family phosphatase"
                     /protein_id="WP_156727770.1"
                     /transl_table=11
                     /translation="MTYQLRRLSLAAVNIDGVLLNDTFSPVIHSFLTRRGAVYTPDVER
                     AVLSQPRRIAGEQMARLTGSDATSEDMLSAYFEERDSYLREHPVQLTEGAVGLLRRLRG
                     LGLRTVCYGGLSKEHFDRYLGEYAELFDGPGYVCTNDFRPGIREITGEVFGLTPGEALF
                     IDDVARVAEAARELGVPFIGHPSSFEHSFQHRLMAEAGVRHLVSSLDAIDEELVRVLDA
                     EAVSETVWRTHEGEGAPR"
     gene            7486..8259
                     /locus_tag="GCN81_RS30505"
     CDS             7486..8259
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (10-180). E-value: 1.6e-11.
                     Score: 36.4. Matches aSDomain:
                     nrpspksdomains_GCN81_RS30505_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891482.1"
                     /locus_tag="GCN81_RS30505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_156727771.1"
                     /sec_met_domain="adh_short (E-value: 1.6e-34, bitscore:
                     114.7, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.1e-33, bitscore:
                     112.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGQGLLAGKVAMITGASSGIGAAAARLFAAEGAAVVLMARRKDR
                     LEALAGEISAAGGRALAVPGDVTSGGDVRRTVEAAVERFGGLDAAFNNAGYATVEQSLH
                     QTDDEVFDRTIDVNVRGVWNCLRHQVPAMLDSGRGGSVVNTSSVAGRYATGASAPYVAA
                     KHAVLGLTRAAAADYGQKGIRVNALVVGSTRTEMMEQVLEGSAALEEVFVASQIQKRMA
                     APREVAEAAVWLCSERSSFVTGVAMPVDGGCGAQA"
     aSDomain        7516..8025
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS30505_PKS_KR.1"
                     /evalue="1.60E-11"
                     /label="GCN81_RS30505_PKS_KR.1"
                     /locus_tag="GCN81_RS30505"
                     /protein_end="180"
                     /protein_start="10"
                     /score="36.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAMITGASSGIGAAAARLFAAEGAAVVLMARRKDRLEALAGEISA
                     AGGRALAVPGDVTSGGDVRRTVEAAVERFGGLDAAFNNAGYATVEQSLHQTDDEVFDRT
                     IDVNVRGVWNCLRHQVPAMLDSGRGGSVVNTSSVAGRYATGASAPYVAAKHAVLGLTRA
                     AAADYGQ"
     CDS_motif       7525..7611
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS30505_0001"
                     /evalue="3.30E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="GCN81_RS30505"
                     /protein_end="42"
                     /protein_start="13"
                     /score="13.3"
                     /tool="antismash"
                     /translation="ITGASSGIGAAAARLFAAEGAAVVLMARR"
     misc_feature    8464..8478
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 20.32"
                     /tool="antismash"
     misc_feature    8478..8491
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.3"
                     /tool="antismash"
     gene            8554..9567
                     /gene="gap"
                     /locus_tag="GCN81_RS30510"
     CDS             8554..9567
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /GO_process="GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="gap"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010984421.1"
                     /locus_tag="GCN81_RS30510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="WP_156727772.1"
                     /transl_table=11
                     /translation="MTLRIAINGFGRIGRTVARAALARTDVDIVAVNDLAEPELLAHLF
                     QYDSVHGRLPDAVGVDDGHLVVAGRRIRVLQRRDPAELPWAEMRVDVVVESTGVFTDAA
                     DARRHLDAGARRVLISAPAKNEDLTVVMGVNSDSVEARHTVLSNASCTTNCAALLAKVL
                     DDEYGVLRGLMNTVHAYTSDQRLQDVPHKDYRRARAAAANIVPTSTGAARAIGKVLPRL
                     EGKLDGLSMRVPVIDGSVVDLTVELDAVVTAAQVNAAFRQAAEGGLAGFLEYTEAPIVS
                     TDIVGSPASCTFDSGLTMVSDRPGGGSVVKVVGWYDNEVGFSHRTLDLALLVGDTR"
     misc_feature    complement(9591..9600)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(9632..9648)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.52"
                     /tool="antismash"
     gene            9848..10861
                     /locus_tag="GCN81_RS30515"
     CDS             9848..10861
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529950.1"
                     /locus_tag="GCN81_RS30515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="WP_156727773.1"
                     /transl_table=11
                     /translation="MEPIYVFSDAERGELFSVLQGIGKSPYEDYLTFSRAVTDVIDRGE
                     VPAFFAQACEQIRADRHSGAGQAHVLRNVPLDAEIPVLDLDDPVADKHLKKKTYIGEAL
                     LELFNQLLGTPLLSYGTRFNGDFFIDVIAINKYSGMQTGFSDSELVYHNDRTAHPVRAD
                     HISLLGMRTPDTDYIYTGFVDGRALLAELTGEQQEVLRQPYFHTPYDVFSQDNNGALSV
                     SEDHAILENEHSFRYLDTTTTVTQQAPEAAKDALLALKNALVRAQKQRHRILTGDLFTF
                     ANQDGLHSRDKMEINDPEQARQRWLLKTYAFRDRAAMDRHADKWLDGVAGRVGDGT"
ORIGIN
        1 ccagccgggc ccctgtcttg gtgcagcact tgaaatccag gtgatccggc atgatgaccg
       61 ccatgacgga cgagatggac ctgggggcgg cagcggcgga attcgaccgg accaccgcgg
      121 cggaggccga gaaagccgca gaggacgaac gcaagcaggt gctctcgcag tttcccctgg
      181 agcagtgggt gacgctgccg ctgtctcggt atgccctggg acagaccgcg cagtccggga
      241 gcgggccaac gtactgtcgg ctgatcgagt tcggtacacc gaacctcggc agcatcaagg
      301 gcggcagcgc ggcgaagcac atcatgtacc accacaactc cggcgagtgg cggatagccg
      361 caccgctgcg tgggatggag ccgcaggagt cgtgggagga actgcgcgga cagttcgtgc
      421 gggccttcga agccgtggcc gcgggggact tcgaggccgt gaacgatctg gaagcgctgc
      481 gctacgggca gaccctggtg accaagtcgc tggccactta cttccccgag cacttcctgc
      541 ctatctacgc cggcgagcac ctgcgcaagt tcgtcacgct gctggacggc gccgctgaga
      601 gcgacgcacc cgcctgggac agtaaccgca gactgctgga actcgtacga tcccgtgcgg
      661 agttcgacgg ctggagcggg caggaagtca tgcacttcct gtacgagagg ttcgatccgc
      721 gcccgcagca gcgcaccatc tggaaaatcg cccccggtga acgaggacgg ctgtgggacg
      781 cgtgccgtga cggcggggta atccgcgtgg ggtgggacga gctcggcgac ttcggccagt
      841 accagagcga caccgagctg aagcaggccc tcgacgcaca ctggccgcgc agcagcggcg
      901 gcagcctgac gctcgcccga cggctgctgg ccttccggga cctggaggcg ggagaccgga
      961 tcgtcgctaa ccgcgggatg gacgaggtcc tggccacggg caccgtcaac gggagctacc
     1021 gcttcgacgc cgaccactcc gagtaccggc atgtcgtccc ggtgtcctgg gacgtctccc
     1081 acgcgcggaa actgtcgaag ccccagcacg gctggcggtc cacgttcgcc aaggtgcagc
     1141 cagcgctcat ggcttggatc accgcgggcc tcgccgagtc ggatacagag ccggcgggaa
     1201 cgcacgccgg tagccccggc acgctgcccg aggatgtcgc gggcgtgctg gaggcactgg
     1261 agcgcaaggg gcaggtgatc ctgcacgggc cgcccggtac gggcaagacc cggctggcac
     1321 tcggtaccgc cctcgccctc gccggtcgtg cggacgcgct caactccacg tcggcccggc
     1381 ggtctacggc ccaggccgag atgctgcgga acggccgcat ccgcatggtg accttccacc
     1441 cgtcctacgg ctacgaggac ttcgtggaag gcttcaagcc ggacctgagc gccacgggcc
     1501 ccgggctgac cctggcgctc accgacggcg tgttccacac cctgtgcgcc caggccgccg
     1561 ctcgtccgga cgagaccttc ctactgatca tcgacgagat caaccggggc gatctgccgc
     1621 ggatcttcgg cgagttggtc acccttctcg aactcgacaa gcgcggcctg cccctcgccc
     1681 tgtccgtcag caggcgcacc ttctccgtgc cgtcgaacgt gcggatcatc ggcaccatga
     1741 acacggcgga ccgcagcatc agccatctgg acgccgccgt acgccgccgc ttcgccttcc
     1801 tcccgctcgg ccctgacccg gacgccgtgt ccggcaccgt cggccccctg gaactggcag
     1861 agttcttcga gtccctgaac acccgcatcg cccggcatct cgacgccgac caccagatag
     1921 ggcacgccta cctgctacgc aacggcgagc cgatcgccac cgaggacgac ctggccgccg
     1981 ccttccacca cgaagtgatc ccgctcctgg aggactactg cctcggccgc gccgacctgc
     2041 tgcaccgcat cctcggcggg ctggtcgacg cggacaccgg gcgtcccgcg ctgatgtcac
     2101 cgcaggacct cgccgccgca ctcgcgaccg agttcaccag cggcacgccg ggacccgatg
     2161 cctgaccgca ccgaggtcag gctcgcagag tacggttccg ctctgctcgg ggcggagcag
     2221 ctcacgcccc gggacgtcga gcggctgcgc ggcctccata cacgcggccg cctcagcctg
     2281 gaccaggccc ggtccgggtg gcggctcagg gccgacgcga ccgtgggcat cctggtgctc
     2341 gaccgcatcc gtctggtcat cgagcccaag ttcgccatcc cgggcgagca gctcatgcgc
     2401 tggctcgcct acgccctggg cgcgccggca ccgctcccgg cgacggcgcg gcgctgggcc
     2461 accggcccgg atggctacgc cgacctcgtg gccgcggccc tgctggagca gtgcgagcaa
     2521 ctggtccggg aggggctgcg ccgagactac gtacgccgtc agagcctgga acccgtcctg
     2581 cgaggacgcc tcgacgtcgc ggcccaggtc tcccggcgct acgggcaact cgatcaactg
     2641 catgtccgcg ccttcgacca ggaggcggac atctgggaca accgcgtcct ggggaccgcg
     2701 ctgcgggtgg cccgcgccct ggccacacgt cccgacctgg tccgcgacct ccacgccatc
     2761 gccgacgcgt tcccccaggc ccccaccccc gacgcggcgc tccgcaccct ggaccgcacc
     2821 cggtacacac gcctgaacgc ccgctaccgc acagcgcaca cctgggcccg cctgctgcta
     2881 agcggcggag gcgtgaccga cctcctcacc gacgacggca tgacggcgga cggcctcctg
     2941 ctggccatgc ccgccctctg ggaggccgtc gtccggcgtc tggccaccga ggccgccgcc
     3001 ccacacggcg ggcacaccgt ccccagcagc ggcgcgacgg gcatcaccgt ccggggcgat
     3061 ctcggcagca cctcgacctt ccggcccgac gtgctgctca gccttccggg gcgtgccccg
     3121 tccgcgcgca cactgctgcc ggtggacgcc aagtacaagc gctacgaccg ttacggcgtc
     3181 ggtgccggcg atgtccacca gctcctcacc tacagcgccg gctacacccc gaataccgac
     3241 ttgcgggcag tgatcgtcca cccatgtccc ggcgggcact cccaccgagt cctccaggtc
     3301 agtgggccgc ggggctcgct cggaagcatc cacgtcctgg gcgtcgacac cctcgcggcc
     3361 ccggagcagg cagccgtgtg gatcaggtcg gtagcgccct ggggcgactg gtgatcgtgc
     3421 gacagtccgt gaccgggcca cgagcatgcg agcagtgaac tactcaagac aaccacgcgt
     3481 gaacgccctg agcgctcatc gccgatgccg tctttccact tgccctcggc acttgcgcgg
     3541 ccatcgtcag catcgacaag gtccaggaac ttcagccagc tgtggcatga aagcgcatgc
     3601 cgctggactc gttcgacagc tccgtcgcct catcgagatc gcttacgtca cccaagtccg
     3661 caaccagccg ctccatcgtt cgtcgaggcc agggcctccg gagtcggccc ggcagaacag
     3721 ttcggcgcac ggcggcggtt cgcgtttctc gagttccggt gccgtcaggg atgtcgtcga
     3781 gaaccggctc gtcggccccg ggtgcacagg cacgggaagg gcggacacga cctctggccg
     3841 ttcctcacag ccctcagcgc agccggcctc tacgccgtcg ccgcattcgc cacgatcggg
     3901 tgaatcttcc ccacctgagg caaccacaca acctttgcac cagtcgcaaa cacgcaagcc
     3961 accatccacg cacccctggg gggaacatgc gtcacacccg caccagcgcc atcgccgccg
     4021 ccggactgct ggccctgacc gcatgcggca ccggcgaaga aaccgtcaac accacaccga
     4081 agaagaccgt aaccaagacc gtcgagggag ccgccgacag caccgccagc agcaccccgg
     4141 aggccgagaa gaaggcagcc gccgtcggcg acacccttac cctccgcggc ttcgaggacg
     4201 gcgagcaact cgacgtcaac ctgaagcggt ggctccccac tgccaagcct gccgacgagt
     4261 acgccgtccc gcaggacggc aagcgttggg ccgccgcgca gtttgagctg gtcaatacgg
     4321 gcagcaaggt gtactcggac agcccgtcaa acggtgcgaa ggcggccgac gcgcaggggc
     4381 agcgtttcga ctcctggttc ggggagatca cagcgggtcc ggagatggcg tctcaggtga
     4441 cgctaccgag gggggagaag gcattgggct gggtggtgtt cgaggtaccg aaggggtcga
     4501 agatcgtgag tgtccagttc ggtatgaact cggggttctc ggacgagtcg gggcagtggt
     4561 ccgtgagcta gtccgccgac aggcacgtcg gccggttgcc ctctctccgg cgcgcccgaa
     4621 ccggagcccc cagagcccac caggcccttg gggctccaca tccacccaga cgtccgcgca
     4681 ggaccacccg atcgctcaca gtgccggaat ggctggccgc tcgatgcggg ccgcgccgtg
     4741 gctcgcagtc gacgcgcgcc tcacccaccc tgacgcgccg tggcagcagg gtggctgatc
     4801 acgatggatg cctgctgggg atccaaggcc accccacccc accgcccccg gcgggcaccg
     4861 atgggcccac cgatgggcac gagaaagccc cctgaaaccg cgcttccgca gttcaggggg
     4921 cactatgacg tggagcctag gagagtcgaa cctccaatgc ccttgagggt ggtaaccgca
     4981 ggttcggccc tccagctcga tcatgtgcgc agcttgaggg atgcggtgaa caccgacgtg
     5041 ccgttctgtt ccgcacgcac cctgacaccg agggcgcccg tggtgtcgtc gggacggacg
     5101 caggcctcga ccgagcaggc gctgtcgaac tcgacgtagc gaacgaaggt ggcgtccatg
     5161 gctgtgagga cggtgcgccg ggggtgggcc acggcgttgg cagcctggcg ggcggcctcc
     5221 aggaggagca tgccaggtac gtggtcgacc ggatggtcga agagggtcgg gtgcccgagg
     5281 tcgacgcgga gcaggaagcg gccctcggaa tccgtggagg tgagcaccac gtcgcgctga
     5341 cgcgtgcgtc caaccagctc ggggaccacc ggctcgcccg gcgacagcgc cgtgcgggct
     5401 gtccccgcag gatcggcgcg gccggcgcgc atgcggcggt agagcgcgaa cgggtggttg
     5461 gtgaagccca cgtgggccgt accgaggagc agtccgtcgc cggtcaggtc gatgcccatc
     5521 gtcatggcag acagccgggc ccgtctgcgc tcgatgttcc cgcacgctat ccggagctga
     5581 aggtcggccg ggcggccctg gtcggcaact gcctcctggc cgaggcggaa ccggaagtgc
     5641 tgccagatct ggcggtggcc gaaggggacg tcgtacgcgc tgtgacagag catgggaatc
     5701 gcctggcgta cggattcggc aaggagcagc gggtcatgga gaccgtgggc ggaacggtag
     5761 aaggggtggt cgtgcggcca ggccgcggtg agcgcatggg tgtcgggtcc ggtgggctgc
     5821 cagccagtga ggaggacttc ggcctcgttg aacttgtgta cgtactgctg cggtatgcag
     5881 gtcgggctgg gacctgtgga gggcgacatt ctgtgcgtcc ttgtgcaagt ggtgctgaac
     5941 atgggaaagc gccaggtagg gggctacatg atcgtaaagg tctctatcca ggagcaatca
     6001 cgaccccgca gaggcttggc catgggccga ttgatgtaat gtgaaccctt tgttcttcgt
     6061 ctgcgaggat gcgcacggtg gctgagtcga agcaggagcg ggcagtgcgg acccggcggc
     6121 aggtgctggt gtcggcagcc gagttgttcg acgcgtacgg cttcgcgggc accagcattt
     6181 cgcggatctg cgagcgtgcc gagaccaccg cgggggcgct ctaccatcac ttcggttcca
     6241 aggagggtct ggccaaggcg atcatgaacg cccagccgga cacgatcctt ccgtggctgg
     6301 agtccgaggg actgcagcgg ctggtggaca tcacgctgat ctgggcccat caattgcaga
     6361 cagatccgct tctgcgcgcc ggtgtgcggt tgacggggga gcaggccacc ttcggggtgc
     6421 aggacgcgtc cccgtatgag gactggacca ggatcatgga agaatgcctg ggcgtcgccg
     6481 ccgagaaggg cgagttgcag gcggacgtca caccacggaa cgtggccgag ttcgtcgtgg
     6541 aggcgtgcac aggtgtgcag accgtggcag ccgtgctgac cggccgttcc gatctggcgg
     6601 cccgtgccgt gcgtatgtgg cagttcctac tccccggcat cgcggctaag gacgtggtcg
     6661 tacgcatcga cctcgatccg gcgcgagtcc aggtcgagcc tgtctgaccg ggccggcgac
     6721 tcccgcactg ggcgcccccg catcgttccg ctatccatga ggacttatga cgtaccagct
     6781 ccgccggctc tcactggcgg ctgtcaacat tgacggtgtc ctgctgaacg acaccttcag
     6841 cccggtcatc cacagctttc tcacccggcg cggtgccgtc tacacgcccg acgtcgagcg
     6901 ggcggtcctc tcccagccac gccggatcgc gggcgaacag atggcgcggc tgaccggctc
     6961 cgacgccacg agcgaggaca tgctcagcgc ctatttcgag gagcgggact cctatctgcg
     7021 ggaacacccc gtacagctga cagaaggtgc ggtcggcctg ttgcggcggc tgcgcgggct
     7081 ggggctgcgc acggtctgct acggggggct gagcaaggag cacttcgacc gctacctggg
     7141 cgagtacgcg gaactcttcg acggtccggg ctacgtgtgc accaatgact tccgccccgg
     7201 tatccgggag atcacaggcg aggtgttcgg gctgacgccc ggcgaggccc tgttcatcga
     7261 cgacgtggcg cgtgtcgccg aggcggcgcg agagctgggg gtgccgttca tcggccaccc
     7321 cagcagcttc gagcacagct tccagcaccg gttgatggcc gaggccgggg tacgccacct
     7381 ggtgtcgtca ctggacgcca tcgacgagga gctggtgcgt gtgctggacg cggaggccgt
     7441 cagcgaaacg gtttggcgta cgcacgaggg cgaaggagcg ccgcgatgac cgggcaaggt
     7501 ctgctggcag gcaaggtcgc catgatcacg ggggcgtcga gcgggatcgg cgcggcggcc
     7561 gcacggctgt tcgcggcgga gggcgcggcc gtggtgctga tggcgcgcag gaaggaccgg
     7621 ctggaggcgc tggccgggga gatatcggcg gcgggcggcc gggcgctggc cgtaccgggc
     7681 gatgtgacat cgggcggcga cgtgcggcgc acggtggagg ccgccgtcga gcggttcggc
     7741 ggattggacg cggcgttcaa caacgccggg tacgcgacgg tggagcagtc actgcaccag
     7801 acggacgacg aggtcttcga ccgcacgatc gacgtcaatg tgcggggagt gtggaactgc
     7861 ctgcgacacc aggttcccgc catgctggac agcggcaggg gaggctcggt ggtgaacacc
     7921 tccagtgtcg cgggacgtta cgcgacgggc gcgtcggcac cgtatgtggc agccaagcac
     7981 gcggtgctgg gtctgacacg agccgcggcg gccgactacg ggcaaaaggg catccgggtc
     8041 aacgcactgg tggtcgggag cacccgcacc gaaatgatgg agcaggtgct ggagggttcc
     8101 gccgcactgg aagaggtctt cgtggcaagc cagatccaga agcggatggc ggcgccccgg
     8161 gaggtcgccg aggcagctgt gtggctgtgc agcgagcgct cctcgttcgt caccggtgtg
     8221 gcgatgcccg tggacggcgg ctgcggtgcg caggcctgac tccctgcccc gcaggacgtg
     8281 aattgcccgc cgacttgccg aagtcggcgg gcaattcgct tctcagggat accgtggccg
     8341 gtaaatgtag tggccatgcg tagactccgt aatcgagtcc ggcccgcccc ggccctttcc
     8401 ggcactcgtc ggaatgtcaa cggaaccacg atgaagtctt ccggtcgacc ttcaccggac
     8461 tcctgccagt catttccaga acacacgatc cggccttccg ttacggggca cccaggagct
     8521 ggattcgtgt gcggagaagg gaaaccggag tagatgactc tgcgcattgc catcaacggg
     8581 ttcggccgca tcggccgcac cgtggcacgg gccgcgctgg cccggacgga cgtcgacatc
     8641 gtcgcggtca acgacctcgc cgaaccggag ctgctggcgc acctcttcca gtacgactcc
     8701 gtccacggcc gcctgccgga cgcggtgggc gtcgacgacg ggcatctggt ggtcgcgggc
     8761 cgtcggatac gggtgctcca gaggcgcgac ccggcggaac tgccgtgggc cgaaatgcgg
     8821 gtggacgtgg tggtcgagtc gaccggcgtc ttcaccgacg cggccgacgc ccgccgtcat
     8881 cttgacgcgg gtgcacggcg ggtactgatc tcggcgccgg ccaagaacga ggacctcacg
     8941 gtcgtgatgg gcgtcaactc cgattccgtc gaggcccgcc acacggtgct gtcgaacgcg
     9001 tcgtgcacca ccaactgcgc ggcgctgctg gccaaggtcc tcgacgacga gtacggcgtg
     9061 ctgcgcggcc tgatgaacac cgtccacgcg tataccagtg accagcggct ccaggacgtg
     9121 ccgcacaagg actaccggcg ggcccgggcc gccgcggcga acatcgtgcc cacgagcacg
     9181 ggcgcggcca gggccatcgg gaaggtgctg ccccgtctgg agggcaagct ggacgggctc
     9241 tcgatgcgtg tgccagtgat cgacggttcc gtggtcgacc tgacggtgga gctggacgcg
     9301 gtggtgacgg ccgcgcaggt gaacgcagcg ttccgtcagg ccgccgaggg tggcctcgcc
     9361 gggttcctgg agtacaccga ggcaccgatc gtgtccacgg acatcgtggg ctcgcctgcc
     9421 tcgtgcacct tcgacagcgg gctgacgatg gtcagcgacc gcccgggagg cggctcggtc
     9481 gtcaaggtcg tgggctggta cgacaacgag gtgggcttct cgcaccgcac gctggatctg
     9541 gcgctgctgg tgggcgacac acgctgagac gtacctgccg ccaggacgac ggccgccttc
     9601 ccgcacaggg gaggcggccg tcgtcgcttt ctgcccgctt ttccaccgcc gccatttccg
     9661 tgtctgccct tcgccttttc actgtccacc tttggtggag tcctctcggc gcaggctcca
     9721 ccttccgacg atccaccctt ccgaagatcc acccccggac gatgccgatg ggtatttcac
     9781 tgggcgagaa tccataggac cggaacacag acgcattttt gtgtcacgcg cagagaattg
     9841 gttaccgatg gaacccatct acgtattttc cgatgccgag cgcggggagc tcttctcggt
     9901 cctccagggc atcggcaaga gcccgtacga ggactatctg acgttcagcc gcgcggtgac
     9961 cgatgtcatc gaccggggtg aggttcccgc tttcttcgcg caggcgtgcg agcagatccg
    10021 tgcggaccgg cacagcgggg ccggccaggc gcatgtgctg cgcaacgtgc cgctggacgc
    10081 cgagatcccg gtgctcgacc tggacgaccc ggtcgccgac aagcacctga agaagaagac
    10141 gtacatcggt gaggccctgc tggagctgtt caaccagctg ctgggcaccc cgctgctgtc
    10201 gtacggcacc cgtttcaacg gtgacttctt catcgacgtc atcgccatca acaagtacag
    10261 cggcatgcag accgggttca gcgacagcga gctcgtctac cacaacgacc gcaccgcgca
    10321 cccggtgcgt gccgaccaca tctcgctgct gggtatgcgc accccggaca ccgactacat
    10381 ctacacgggc ttcgtggacg gccgcgccct gctggccgag ctgaccgggg aacagcagga
    10441 ggtgctgcgt cagccgtact tccacacccc gtacgacgtg ttctcacagg acaacaacgg
    10501 tgccctgtcg gtctcagagg atcacgccat cctggagaac gagcacagct tccgctacct
    10561 ggacaccacc accacggtga cccagcaggc gcctgaggcg gcgaaggacg cactgctcgc
    10621 gctgaagaac gcgctcgttc gcgcgcagaa gcagaggcac cgcatcctga ccggcgacct
    10681 gttcacgttc gccaaccagg acgggctgca cagccgggac aagatggaga tcaacgaccc
    10741 ggagcaggcg cggcagcggt ggctgctgaa gacctacgcc ttccgggacc gggcggccat
    10801 ggaccggcat gccgacaagt ggctcgacgg ggtggccggc cgcgtcggcg acggcacctg
    10861 agccgcaccc cgccccatga ccggcggctg gtacgtccga ccggcttcc
//
