LOCUS       NZ_WHOM01000072        10456 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces apocyni strain TRM 66233 516, whole genome shotgun
            sequence.
ACCESSION   NZ_WHOM01000072
VERSION     NZ_WHOM01000072.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces apocyni
  ORGANISM  Streptomyces apocyni
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 402041)
  AUTHORS   Liu,W.
  TITLE     Streptomyces poacynumensis sp. nov., a endogenous actinomycete
            isolated from Poacynum hendersonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 402041)
  AUTHORS   Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Key Laboratory of Protection and Utilization
            of Biological Resources in Tarim Basin of Xinjiang Production &
            Construction Corps, Tarim University, Xingfu Road, Alar, Xinjiang
            843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009739465.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,397
            CDSs (total)                      :: 6,319
            Genes (coding)                    :: 6,205
            CDSs (with protein)               :: 6,205
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 34 of 114
            Pseudo Genes (incomplete)         :: 100 of 114
            Pseudo Genes (internal stop)      :: 6 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:56:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 177891
            Orig. end                         :: 188347
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHOM01000072.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10456
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="1"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10456
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10456
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5456](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5456)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            1325..2218
                     /locus_tag="GCN81_RS26125"
     CDS             1325..2218
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155857.1"
                     /locus_tag="GCN81_RS26125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_156726949.1"
                     /transl_table=11
                     /translation="MSAMPLALLLATAATTAVGAAALHAVYGLRKQLAEITALRAELAA
                     SVAQRQQHAASVPSARDASDAPDTTAIRTAVTEALAEERERELAEARAFWAAQEARDAA
                     DAPSLLTGLPPSIAEELFLPRQADFAGVERELFSEIVHPAEESAERAETEFAEFAETAE
                     HTEFADDSPELAAARRRHPSHPDFVPVQPVRDSAAGDHELTVSRLDELAQARTALSDVR
                     PGPLGTLDVYVFSDGTTLCMTPGHRETAERLSAALRDGETPVLLGGSGISGAYTLTFAC
                     GGENVYILADRVIASA"
     gene            complement(2254..2565)
                     /locus_tag="GCN81_RS26130"
     CDS             complement(2254..2565)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987138.1"
                     /locus_tag="GCN81_RS26130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_197287784.1"
                     /transl_table=11
                     /translation="MLDTTPLTSAVDRFADRLRAAPQSRLQRGAAAEALALARELALRA
                     QRIEAPETPAHEMPDAGMFAAADQVVVAGRDLALVLMDEGELADALDLVAQAQKRAGV"
     misc_feature    complement(2646..2660)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.66"
                     /tool="antismash"
     gene            2704..2934
                     /locus_tag="GCN81_RS26135"
     CDS             2704..2934
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338122.1"
                     /locus_tag="GCN81_RS26135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4287 domain-containing protein"
                     /protein_id="WP_156726950.1"
                     /transl_table=11
                     /translation="MSLVFSEETHRNLLARIPHCTGREVSDWLRAVDDGPAFLRFEEKV
                     SWLRGEYDLAHGHAKAIVHEHDMRRAARRLS"
     gene            complement(3115..3981)
                     /locus_tag="GCN81_RS26140"
     CDS             complement(3115..3981)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605170.1"
                     /locus_tag="GCN81_RS26140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="WP_156726951.1"
                     /transl_table=11
                     /translation="MRSSNPVFSRRGFSRDNGHPGFNAGPQAGGPAFGNQSNPYANQGN
                     PYTQAPQAPTNPYAPQGTPQAPPAAGRMTMDDVVTRTGLTLGVLIAGATVAWVTDMGLG
                     LAIGAALIAMVLGFVQAFKQKPSPPLIIGYAIFEGLFLGAISNYVNTFIASGAAMQAVL
                     GTMAVFGTMLFLYKTRIVRVTARFTRVLMIAAMSFMVLTAVNLLFMVFAGGDGLGFRSG
                     PLGIVFGVVGVLLGAFFLALDFKQVEDGIAYGAPREESWLAAFGLTMTLAWIYLEMLRL
                     VAILQGD"
     gene            complement(4122..4943)
                     /gene="melC2"
                     /locus_tag="GCN81_RS26145"
     CDS             complement(4122..4943)
                     /EC_number="1.14.18.1"
                     /GO_function="GO:0004503 - tyrosinase activity [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="melC2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987919.1"
                     /locus_tag="GCN81_RS26145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase MelC2"
                     /protein_id="WP_156726952.1"
                     /transl_table=11
                     /translation="MTVRKNQSALTRTEKRRFVDALLELKRSGRYDEFVTTHNGFIMSD
                     TDQGERVGHRSPSFLPWHRRFLIDFERQLQEVDPEVALPYWDWTVDRTPSASLWADDFL
                     GGSGRPGDGKVMSGRFAEASGAWEINIRVDQRPYLRRALGTGARQLPTRAEVDAVLAMP
                     VYDASPWNSASTSFRNHLEGWRGANLHNRVHVWVGGQMTTGVSPNDPVFWLHHAFVDKL
                     WADWQARHPDSGYLPTTRTRNVVSLHDTMRPWNDVTPADMLDHTPYYTFDA"
     gene            complement(5001..5456)
                     /gene="melC1"
                     /locus_tag="GCN81_RS26150"
     CDS             complement(5001..5456)
                     /codon_start=1
                     /gene="melC1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267951.1"
                     /locus_tag="GCN81_RS26150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="apotyrosinase chaperone MelC1"
                     /protein_id="WP_156726953.1"
                     /sec_met_domain="melC (E-value: 1.5e-41, bitscore: 136.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEISRRRVLGMSAGTFAGLAVAATAAVNATAAKAAPSERQGSQG
                     SGLEAFDEVYQGRRIQGAPTRGAGSHGGHGGGHGSGHGGGHGGQAHDFGYAVHLDGDEL
                     HIMRNADGTWISVVNHYETFATPRQLARAAVTELHGADLVPLVLTSA"
     misc_feature    5659..5672
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.73"
                     /tool="antismash"
     gene            complement(5725..5883)
                     /locus_tag="GCN81_RS26155"
     CDS             complement(5725..5883)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GCN81_RS26155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_156726954.1"
                     /transl_table=11
                     /translation="MPRDGDSVTAVNAHHHHAINCRIDGFGVMPLTSTEGIGRPGRFRT
                     AFGRLMP"
     tRNA            complement(5962..6044)
                     /anticodon=(pos:complement(183901..183903),aa:Leu,seq:taa)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="GCN81_RS26160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Leu"
     gene            complement(5962..6044)
                     /locus_tag="GCN81_RS26160"
     misc_feature    complement(6118..6134)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 19.04"
                     /tool="antismash"
     misc_feature    6268..6286
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 24.91"
                     /tool="antismash"
     misc_feature    6366..6380
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.51"
                     /tool="antismash"
     gene            6423..7193
                     /locus_tag="GCN81_RS26165"
     CDS             6423..7193
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987131.1"
                     /locus_tag="GCN81_RS26165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_156726955.1"
                     /transl_table=11
                     /translation="MTTTPLAHQATAVAARATDLSKVYGQGETQVVALDQVTVDFEQAR
                     LTAIMGPSGSGKSTLMHCVAGLDSFSSGSVRIGDTELGSLKDKQLTKLRRDKIGFIFQA
                     FNLLPTLTAAENITLPMDIAGRKPDKQWMAQVIEMVGLSGRLSHRPSELSGGQQQRVAV
                     ARALASKPDIIFGDEPTGNLDSRSGAEVLGFLRNSVRELGQTVVMVTHDPVAAAYADRV
                     VFLADGRIVDELREPTADGVLDRMKRFDAKGRTS"
     gene            7337..9874
                     /locus_tag="GCN81_RS26170"
     CDS             7337..9874
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1243: ABC
                     transporter, permease component"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382556.1"
                     /locus_tag="GCN81_RS26170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_156726956.1"
                     /transl_table=11
                     /translation="MFRTALRNVLAHKARLLMTVIAVMLGVAFVSGTLVFTNTISQAYQ
                     NSSAKGFDHVDVVIQPENDDDATGRPGDAPRLTQELLEQAGQVPGAASATGTVSGFAAI
                     ADKDGKLVGEGWDSRGANYYPGKDGEDVRYPMKDGKAPQGENEIALDAKTADRAGYKVG
                     DPVRMSIDGPVIEPKLSGVFTTDDGNVAAGGSLALFDTPTAQQLYAKPGEFSEIEVQAK
                     SATSQAALKAEIDKILPKNEAEATTGKKLADDQAQEISDGMSGIRSTLLGFAGVSLFVG
                     IFIIANTFTMLVAQRTKELALLRAVGASRRQVTRSVLFEALVVGTVAAITGLAAGVGIG
                     AGLRGLMGSIGATVPDGPLVFTTGTVITALLVGVVVTMLAAWLPGRRAAKIPPVAAMSS
                     VHATASTKSLVVRNSIGAVFAAAGVAGVLGGTTMGGDGKELMGLGAVLLLIGVFVLTPL
                     LSRPLIAAVAPVMRLFGVSGKLARQNSVRNPRRTAATASALMIGLTLITGMTVIAGSVQ
                     KSIDKMATEALSADYFVSMANYTPLSTDVEKQLAQADGVTATTPLRTSPAHIKGENETL
                     TGVNAQTIGKLTKLDFAQGSFDAMGGDKIVVDSDTAEDHGWKNGSEFTVTYEDGEKGTL
                     RVAGVYEGNEMINGIMLDKDTLAPHQREVTDMQLMLKTADGASPAAKDALKKELGNNPA
                     ILVQDKKDVSEAIAQVFTLLLNMLYGLLAMAVIVAVLGVINTLAMSVFERSQEIGMLRA
                     IGLDRRGIKRMVRMESLVISLFGGVLGIGLGVFFGWAVGELIASALSTYELVVPWGRMS
                     VFLAMAAVVGVLAALWPARRAAKLNMLAAIKAE"
     misc_feature    10424..10438
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 cggatcgtcg ccgtgcagat ggggatggac cacctccccg gctgcaccat cacccgctac
       61 tgctcctcct cgctgcagac cagccggatg gcgctgcacg cgatcaaggc cggtgagggc
      121 gacgtcttca tctcggccgg tgtggagatg gtgtcccgct ccgtcaaggg gtcgagtgac
      181 gggatgcccg acacccacaa cccgctgttc gccgaggccg aggcgcgtac cgccgccgtc
      241 gcggagagcg agggcgccag ctggcacgac ccacgcgtgg acgggctcgt ccccgacgcg
      301 tacatcgcga tggggcagac ggccgagaac ctggcccgcc tcaagggcgt cacccgcgag
      361 gagatggacg agttcggcgt acggtcccag aacctcgccg agcaggccat cgccaagggc
      421 ttctgggagc gcgagatcac cccggtgacg cttccggacg gcacggtcgt cagccaggac
      481 gacgggccgc gcgccggggt caccctggag ggtgtgcagg gcctgaagcc cgtcttccgc
      541 cccgacggcc tggtcaccgc cgccaactgc tgtccgctca acgacggcgc cgccgcgctc
      601 gtgatcatga gcgacaccaa ggcgcgcgag ctgggcctga cgccgctcgc ccggatcgtc
      661 tccaccggcg tctccggcct ctcccccgag atcatggggt acgggccggt cgaggcctcg
      721 aagcaggcgc tgcgccgcgc cgggctcggc atcggcgaca tcgacctggt cgagatcaac
      781 gaggctttcg ccgcgcaggt catcccctcg taccgcgacc tgggtatcga cctcgacaag
      841 ctgaacgtga acggcggggc gatcgcggtc gggcacccct tcggtatgac cggcgcgcgg
      901 attaccggca ccctgatcaa cagcctgcag ttccacgaca agcagttcgg tctggagacc
      961 atgtgcgtgg gcggcggcca gggcatggcc atggtcatcg agcgcctgag ctgaagcagc
     1021 tgacgtcctg agctgaactc gtggcgggga cggcctccgg ggggcgcgcg gcagctctcc
     1081 ccgggggcgc tttcccgcca caggcggcgc ccagaggctc ccgagcccca gccgtgactc
     1141 aatctcctcc aggatgtgac cttcgtcccc cggtaaggcc atttccgcag gtcagggcag
     1201 tctggggcta aacgtcgggt cgaaagacct gtccctttcg tgacctaatg cactgacact
     1261 attgagacgc ctccgacaag ctgaggtaga agtcgggggt cgacttgaaa ccgggagtac
     1321 gtcagtgagc gccatgcctc ttgccctgct gctcgccacc gccgccacca cggccgtggg
     1381 cgccgctgct ctgcacgccg tgtacggact gcggaagcag ctcgctgaga tcacagccct
     1441 gcgtgccgag ctggccgcga gcgtcgctca gcggcagcag cacgccgcgt cggtgcccag
     1501 cgcccgggac gcgtccgacg cccccgacac caccgccata cgtaccgccg tcaccgaggc
     1561 actggccgag gagcgggagc gggagctggc cgaggcgcgg gcgttctggg ccgcgcagga
     1621 ggcccgcgac gccgcggacg ccccgtcgct gctcaccggg ctgcccccga gcatcgccga
     1681 ggagctgttc ctgccccggc aggcggactt cgccggtgtg gagcgggagc tgttcagcga
     1741 gatcgtccac ccggccgagg agtcggcgga gcgcgccgag acggagttcg cggagttcgc
     1801 cgagaccgcc gagcacaccg agttcgccga tgattcgccc gagctggccg cggcccgccg
     1861 tcgacacccg tcgcaccccg acttcgtacc ggtgcagccc gtacgggact cggccgccgg
     1921 tgaccacgag ctgacggtga gccgcctcga cgagctggcg caggcccgta ccgcgctctc
     1981 cgacgtccgc ccggggccgc tcggcaccct ggacgtctac gtcttcagcg acggcaccac
     2041 gctctgcatg accccgggcc accgggagac ggcggagcgc ctctccgcgg ccctgcggga
     2101 cggtgagacc cccgtactgc tcggcggctc cggcatctcg ggcgcgtaca cgctgacctt
     2161 cgcctgcggt ggcgagaacg tgtacatcct ggcggaccga gtcatcgcca gcgcctgacc
     2221 ctcagcgccc cctacgctcc ccacgcgccc cggctacacg cccgcgcgct tctgtgcctg
     2281 cgccaccagg tccagcgcat ccgcgagttc accctcgtcc atcagtacga gggccagatc
     2341 ccgccccgcg accacgactt ggtcggccgc cgcgaacatc ccggcgtccg gcatctcgtg
     2401 cgccggtgtt tccggcgcct cgatgcgctg ggctctgagc gccaactccc tggccagcgc
     2461 cagcgcttcg gccgccgcgc cccgctgcag gcggctctgc ggggcggccc gcagccggtc
     2521 ggcgaagcgg tccacggcac tggtcagagg cgttgtatca agcaccccgc gaccctatgc
     2581 gccggaacgg gactgttgcc aacaccggga cactcaggca cggtgacgtg aaggacccgt
     2641 gagccaggcc cggtcggccc ggcccggtaa cccctcctgc atcgactgcg tccggaggcg
     2701 ccgatgtccc ttgtcttctc cgaggagacc caccgcaacc tgctcgcccg catcccccat
     2761 tgcaccggcc gtgaagtctc cgactggctt cgcgcggtag acgacggtcc cgccttcctc
     2821 cgcttcgagg agaaggtcag ctggctccgt ggcgagtacg acctcgccca cggccacgcc
     2881 aaggcaatcg tccacgagca cgacatgagg agggccgcgc gcaggctgag ctgaggcctg
     2941 tgccgccagc gccagcaacg acccacccgt acgcagacat ccgcacccag acacccgcac
     3001 ccaggtagca cccgtacgca ggtacccgta cgcagacagc gcgaagggcc cgcggatcgc
     3061 tccgcgggcc cttctcacgt agtacgggtc ctgccggtac tgctaccgcc gctgctagtc
     3121 gccctgcagg atggcgacca ggcgcagcat ctccaggtag atccacgcca gcgtcatggt
     3181 gaggccgaag gccgccagcc aggactcctc gcgcggagcg ccgtacgcga tgccgtcctc
     3241 gacctgcttg aagtccaggg cgaggaagaa cgcgccgagc agcacaccga cgacaccgaa
     3301 gacgatgccg agcgggccgc tgcggaagcc gaggccgtca ccaccggcga agaccatgaa
     3361 cagcaggttc acggccgtca ggaccatgaa gctcatggcc gcgatcatca ggacgcgcgt
     3421 gaagcgcgcg gtgacccgga cgatcctcgt cttgtagagg aacagcatgg ttccgaagac
     3481 ggccatggtg ccgagcacgg cctgcatggc ggcgccggac gcgatgaacg tgttgacgta
     3541 gttactgatc gcgccgagga agaggccctc gaagatcgcg taaccgatga tcagaggcgg
     3601 cgacggcttc tgcttgaagg cctggacgaa gcccaggacc atcgcgatca gtgcggcacc
     3661 gatagccagg ccgaggccca tgtccgtgac ccaggccacg gtcgcaccgg cgatcagaac
     3721 gccgagcgtc aggcccgtac gggtgacgac gtcgtccatc gtcatccggc cggcggcggg
     3781 cggcgcctgc ggcgtgccct gcggcgcgta ggggttggtg ggcgcctggg gcgcctgggt
     3841 gtacgggttg ccctggttcg cgtaagggtt gctctggttt ccgaaagcgg gtcccccggc
     3901 ctgcggcccg gcattgaagc ccgggtggcc gttgtcgcgg ctgaaccccc gtcgcgagaa
     3961 gaccgggttg ctgctcctca tctcactcct ccgtggccac cctgtgcggc cttgggttaa
     4021 gggtaatggc ttcgcaaaag ttgcacccta ctgctgtagg aggatctttc ccacaccaac
     4081 cctgacgtca gcacgttcct cgcgctcctc gcgctcctcg gctacgcgtc gaacgtgtag
     4141 tagggagtgt ggtcgagcat gtccgccggg gtgacgtcgt tccacggacg catggtgtcg
     4201 tgcaggctca cgacgttgcg cgtccgcgtg gtcggcaggt agccggagtc ggggtgccgg
     4261 gcctgccagt cggcccacag cttgtccacg aaggcgtggt gcagccagaa gaccgggtcg
     4321 ttgggcgaga caccggtggt catctgtccg cccacccaca catgcacacg gttatgcagg
     4381 ttggcgccgc gccagccctc caggtggttg cggaagctgg tggacgcgct gttccacggc
     4441 gaggcgtcgt agaccggcat cgcgaggacg gcgtcgacct ccgcgcgggt gggcagttgg
     4501 cgagccccgg taccgagagc tcggcgcagg tacggccgct ggtcgacacg tatgttgatc
     4561 tcccacgcgc cggacgcctc ggcgaaccgg ccgctcatca ccttgccgtc gccgggccgc
     4621 ccgctcccgc cgaggaagtc gtccgcccag agggaggcgc tgggcgtacg gtccacggtc
     4681 cagtcccagt agggcagggc gacctccggg tccacctcct ggagctgacg ctcgaagtcg
     4741 atgaggaatc tgcggtgcca ggggaggaag gacggcgagc ggtggccgac ccgctccccc
     4801 tggtcggtgt cgctcatgat gaagccgttg tgcgtggtga cgaactcgtc gtagcggccc
     4861 gacctcttga gttccaggag cgcgtcgacg aagcggcgct tctcggttcg ggtgagggcg
     4921 gactggttct tgcgtacggt catgtcggtg ctccgggtcg gatcaggtca cgtcaggtca
     4981 ggtcaggtca ggcggacggg tcaggcggag gtgaggacga gcgggacgag gtccgcgccg
     5041 tgcagttccg tgaccgcggc gcgggcgagc tgacgtggcg tggcgaacgt ctcgtagtgg
     5101 ttcacgacgc tgatccaggt gccgtcggcg ttccgcatga tgtgcagctc gtcgccgtcc
     5161 aggtggaccg cgtagccgaa gtcgtgcgcc tggccgccgt gaccacctcc gtggccgctt
     5221 ccatggccac ctccgtgacc accgtggcta ccggcacccc gcgtgggcgc cccctgtatg
     5281 cggcggccct gatacacctc gtcgaacgct tcaaggccgg agccttggga gccctgtcgc
     5341 tcggacggcg cggccttcgc ggccgtggcg ttcacggctg ccgtggcggc gacggcgaga
     5401 cccgcgaacg tacccgcgga catgccaagg actcggcggc gactgatttc gggcatgggt
     5461 gttccccccg ggggatcgtc tgactgcgat gaagcgccgg ggatggtcgg ccccccggcg
     5521 gcagcccatg cctacgcgac ggagtgagct cccttgaaat cgccgggagc cgctgggcct
     5581 atatacggac aagccccaac acgatgtacg gaattgaacg aagatgatct tgctgtcagc
     5641 gcaccatccg gcacgcggca aacaaaaatt cctcctaccc caggggctcc aggaaggaag
     5701 atttacggtt catgatcgat cacctcatgg catcaacctg ccaaaagctg tcctgaagcg
     5761 tccgggacgg ccgatgcctt ccgtggacgt gagcggcatc acgccgaagc cgtcgatccg
     5821 gcagttgatg gcgtggtggt ggtgcgcgtt gacggccgtc acactgtcgc cgtcccgcgg
     5881 cacagcggtt atcatcgcga cccaaaagca tgacggcgcc agccagccgc cttcgctggt
     5941 caggcgccgt cgcggcggaa gtgcccggaa ccggacttga accggtacgc ccccgagggg
     6001 cagcgaggtt taagctcgcc gtgtctgcat tccaccatcc gggcaggccg ttggctccgt
     6061 ggttacgcga tcaaagccta tccgggcaca tcccccgaac agcggcggga tggcacgatg
     6121 ttgtcttatt ttattggcgc ctgagggtgc atcagccacg ggaaacgagc ataggcactt
     6181 gccaggggcg gttccccgac cttgcgaaca tccagcagga ttgacgggat tccaccgtct
     6241 tgggcggccc tccggccgag cagcgtctca gggtcggctc tcctacccag gtatgaggcg
     6301 aaggggcaaa catcagccct gggaggaagg gcggaacggg agctgcggtt gactccacgg
     6361 cgggaatcag gcccgacgat ggaagggtcc gctcagccgc ctgtcgacag gagacccgtc
     6421 ccgtgaccac cactcccctt gcccaccagg caactgccgt agccgcgcgt gcgacggacc
     6481 tgtccaaggt ctacggacag ggtgagaccc aggtggtcgc cctggaccag gtcaccgtcg
     6541 acttcgagca ggcccggctg acggcgatca tgggcccctc cgggtccggc aagtcgacgc
     6601 tgatgcactg cgtcgccggg ctcgactcgt tcagcagcgg gtccgtacgg atcggcgaca
     6661 ccgagctggg ttcgctgaag gacaagcagc tcaccaagct gcgccgggac aagatcggct
     6721 tcatcttcca ggcgttcaac ctgctgccga cgctgaccgc ggcggagaac atcaccctgc
     6781 cgatggacat cgccgggcgt aagcccgaca agcagtggat ggcgcaggtc atcgagatgg
     6841 tggggctctc cgggcgcctc agccaccggc caagcgagct ctccggcggc cagcagcagc
     6901 gcgtcgcggt ggcgcgtgcg ctggcctcca agcccgacat catcttcggc gacgagccga
     6961 ccggaaacct ggactcccgc tcgggcgccg aggtactcgg cttcctgcgg aactcggtac
     7021 gggagctggg ccagaccgtg gtgatggtca cccacgaccc ggtggccgcc gcgtacgcgg
     7081 accgcgtggt cttcctcgcc gacggccgca tcgtcgacga actgcgcgag ccgaccgcgg
     7141 acggcgtact cgaccggatg aagcgcttcg acgcgaaggg ccgcaccagc tgagcagccg
     7201 accaactgag cagtcgacca actggcagtc gaccaactga gcagttgacc agctgagcaa
     7261 ttcgaccggc tgagcagtgt gaccagctgc ccagctgacc gccgccccgc gcccgcacct
     7321 ctccccagga cttcccatgt tccgtaccgc cttgcgcaac gtactcgcgc acaaggccag
     7381 gctgctgatg accgtgatcg ccgtgatgct cggcgtggcc ttcgtctccg gcaccctggt
     7441 gttcaccaac acgatctccc aggcctacca gaacagttcc gccaagggct tcgaccacgt
     7501 cgacgtggtc atccagcccg agaacgacga cgacgccacc ggcaggcccg gggacgcccc
     7561 caggctcacc caggagctgc tcgagcaggc cgggcaggtg ccgggagcgg cctccgcgac
     7621 cgggaccgtg tccggcttcg cggcgatcgc cgacaaggac ggcaagctgg tcggcgaggg
     7681 ctgggacagc cgtggcgcca actactaccc gggcaaggac ggcgaagacg tccgctaccc
     7741 gatgaaggac ggcaaggccc cgcagggcga gaacgagatc gccctggacg ccaagaccgc
     7801 cgaccgggct ggctacaagg tcggcgaccc ggtacggatg tccatcgacg gaccggtcat
     7861 tgagccgaag ctgtccggcg tcttcaccac cgacgacggc aacgtcgccg ccggcggcag
     7921 cctcgccctc ttcgacaccc cgaccgccca gcagctctac gcgaagccgg gcgagttcag
     7981 cgagatcgag gtccaggcga agtccgccac ctcgcaggcc gcactcaagg ccgagatcga
     8041 caagatcctg ccgaagaacg aggccgaggc caccaccgga aagaaactgg ccgacgacca
     8101 ggcccaggag atctccgacg gcatgagcgg catccgcagc acgctgctcg gcttcgccgg
     8161 tgtctcgctc ttcgtcggca tcttcatcat cgccaacacc ttcaccatgc tggtcgccca
     8221 gcgcaccaag gagctggcgc tgctgcgcgc cgtcggcgcc agccgccgcc aggtgacccg
     8281 ttccgtgctg ttcgaggccc tggtggtcgg tacggtcgcg gccatcaccg gtctggccgc
     8341 cggtgtcggc atcggcgccg ggctgcgcgg cctgatgggc agcatcggcg ccacggtccc
     8401 ggacgggccg ctggtcttca ccaccggcac ggtcatcacc gcgctcctcg tcggtgtcgt
     8461 ggtcaccatg ctggccgcct ggctgccggg ccgccgcgcc gcgaagatcc cgccggtggc
     8521 ggcgatgagc agcgtgcacg ccacggcgag caccaagtcc ctggtcgtac ggaactccat
     8581 cggcgccgtg ttcgccgccg ccggtgtcgc gggcgtcctg ggcggcacca cgatgggcgg
     8641 tgacggcaag gagctgatgg gcctgggcgc cgtactgctg ctgatcggcg tcttcgtcct
     8701 gaccccgctg ctgtcccgtc cgctcatcgc ggccgtggcc cccgtcatgc gactgttcgg
     8761 cgtctccggc aagctggccc ggcagaactc cgtacgcaac ccgcggcgta ccgcggcgac
     8821 cgcgtccgcg ctgatgatcg gcctgaccct gatcaccggc atgacggtga tcgcgggcag
     8881 cgtgcagaag tccatcgaca agatggccac ggaggccctc tcagccgact acttcgtctc
     8941 gatggcgaac tacaccccac tctccacgga cgtggagaag cagctggcgc aggccgacgg
     9001 ggtcacggcc accaccccgc tgcgcacctc acccgcgcac atcaagggcg agaacgagac
     9061 gctgacgggc gtcaacgccc agaccatcgg caagctcacc aagctggact tcgcgcaggg
     9121 ctcgttcgac gccatgggcg gcgacaagat cgtcgtggac agcgacacgg ccgaggacca
     9181 cggctggaag aacggctcgg agttcaccgt cacgtacgag gacggcgaga agggcaccct
     9241 gcgcgtcgcg ggcgtctacg agggcaacga gatgatcaac gggatcatgc tcgacaagga
     9301 caccctcgcc ccgcaccagc gcgaggtcac cgacatgcag ctgatgctga agacggcgga
     9361 cggcgcgagc ccggcggcca aggacgccct gaagaaggag ctggggaaca accccgccat
     9421 cctcgtccag gacaagaagg acgtctccga ggccatcgcc caggtcttca cgctgctcct
     9481 gaacatgctg tacgggctgc tggcgatggc ggtgatcgtc gccgtgctcg gcgtgatcaa
     9541 caccctcgcg atgtcggtct tcgagcggtc ccaggagatc gggatgctgc gggcgatcgg
     9601 cctggaccgc cgtgggatca agcgcatggt gcgcatggag tccctggtga tctcgctctt
     9661 cggcggggtg ctgggcatcg ggctcggggt gttcttcggc tgggcggtcg gtgagctgat
     9721 cgcgagcgcg ctgtcgacgt acgagctggt ggtgccgtgg ggccggatga gcgtcttcct
     9781 ggcgatggcc gcggtggtcg gtgtgctggc ggcgctgtgg cccgcgcggc gggcggcgaa
     9841 gctgaacatg ctggcggcga tcaaggcgga gtagcggact ggtcgtacgt gtgagggggc
     9901 cccggactgt gtccggggcc ccctcctgtg tcacggggtc aggtccctcc ggtttcccct
     9961 tctgggttcc agttccgggt gcgcagtggc aggcccgagt cgcccgatgc cgtgggcttg
    10021 gcggccagga tctggttgac gccgatgcgg ttgctctcga aggccagcgc ggacgcggcc
    10081 atgtacaggc gccagacgcg agcgcggccc ggggacgaga ggcgtaccgc ctcgggccag
    10141 cgggcctcca ggttggcgac ccaccgacgg agcgtcagag cgtagtgctc gcggagcgct
    10201 tcgaggtcgc ggacctcgaa cccggcccgc tccaactgcg cggtggtgcg gccgacgggg
    10261 gcgagttcgc cgtcagggaa gacgtacgcg tcgatgaacg cgttgatgcg gtagtcggat
    10321 tcgtcgtcct gggggcgacg ggcgatctgg tggttcagca gtcggccgcc cggcttgagg
    10381 agggccaaca ggtcgcgggc gtactcggcg tagcgctcgg agccgacatg ctcggccatc
    10441 ccgatcgagg agatcg
//
