LOCUS       NZ_WHOM01000071         5201 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptomyces apocyni strain TRM 66233 515, whole genome shotgun
            sequence.
ACCESSION   NZ_WHOM01000071
VERSION     NZ_WHOM01000071.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces apocyni
  ORGANISM  Streptomyces apocyni
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Liu,W.
  TITLE     Streptomyces poacynumensis sp. nov., a endogenous actinomycete
            isolated from Poacynum hendersonii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2019) Key Laboratory of Protection and Utilization
            of Biological Resources in Tarim Basin of Xinjiang Production &
            Construction Corps, Tarim University, Xingfu Road, Alar, Xinjiang
            843300, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009739465.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 17:09:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,397
            CDSs (total)                      :: 6,319
            Genes (coding)                    :: 6,205
            CDSs (with protein)               :: 6,205
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 34 of 114
            Pseudo Genes (incomplete)         :: 100 of 114
            Pseudo Genes (internal stop)      :: 6 of 114
            Pseudo Genes (multiple problems)  :: 23 of 114
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:56:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5201
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            WHOM01000071.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5201
                     /collection_date="2018-07"
                     /db_xref="taxon:2654677"
                     /geo_loc_name="China: Xinjiang"
                     /host="Apocynum hendersonii"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces apocyni"
                     /strain="TRM 66233"
                     /type_material="type strain of Streptomyces apocyni"
     gene            complement(<1..>5201)
                     /locus_tag="GCN81_RS25320"
     region          1..5201
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..5201
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5201
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:5201](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..5201)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..5201)
                     /NRPS_PKS="Domain: Condensation_DCL (0-315). E-value:
                     8e-59. Score: 191.1. Matches aSDomain:
                     nrpspksdomains_GCN81_RS25320_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (483-881). E-value: 6e-108.
                     Score: 353.0. Matches aSDomain:
                     nrpspksdomains_GCN81_RS25320_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (990-1056). E-value: 1.5e-20. Score:
                     65.2. Matches aSDomain: nrpspksdomains_GCN81_RS25320_PCP.1"
                     /NRPS_PKS="Domain: Condensation_DCL (1090-1392). E-value:
                     3.7e-61. Score: 198.8. Matches aSDomain:
                     nrpspksdomains_GCN81_RS25320_Condensation_DCL.2"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="GCN81_RS25320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_156726809.1"
                     /sec_met_domain="AMP-binding (E-value: 1.1e-116, bitscore:
                     375.8, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.4e-15, bitscore:
                     43.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 4.7e-48, bitscore:
                     149.8, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYPMAELQLGMVYEMERDPDRNPYLNVTSLRLPGRFDESAFRTAV
                     SKVVRRHPALRTSFALSSLSEPMQLVHAEADLPVVVTDVTGQDSEVQRSVLANHVASER
                     ARGFDTASAPLCRMSVHVLSDDAFQWTVTDHHAILDGWSLASTLAEITDTYHALLQGED
                     VTLPPLRSTYRDYIAAERTALTTTESTTFWHTLLAERPQATLPRWPTDRPGHPSLTGER
                     QEGESHHHDGDTGRGALVSVVPADLVTELETVAGRIGVPLKAVLLAAHLRVLGFVTGGA
                     DVVTGLSSNGRLEETDGSEVRGLFLNSLPFRLRLSDGSWADLARAVFAAERAALPHRRY
                     PMPALQRALGGEPLFETGFVYNHFRHVEDLADSGRAAVSISESYDEIAAGVARTSFPLH
                     VAMSREPGETGMRVELEYDARQFAAEQVRLLRDYHLRALTALAGTPDADHRQTDLLSSS
                     EHALLADWNDTATPVPDTPVHELVAARATATPERTAVIVGDRSLTYAELDALANRLAHR
                     LRDLGAGPETYIGVCLERSPELIVTLLAVLKAGGAYVPMDPGFPAARLEHMAHQADVRV
                     LLVAPGTADRVPAGPWQTVVLDDTVLVDDGVPREAPENGAGPDNACYTIFTSGSTGLPK
                     GVVTRHRNVTELLYGGAAMDLREDDTLLQIATVAFDVSTFEIWAPLVAGARLVLAPSGP
                     YETGDLAEWIARYDVSVLHATASLFALLMDQQPQALDGLRKLLTGSETVSPAHVARAVA
                     RHPALEVVNCWGPTETTTFSVCGTFTAENLPAGPLPLGTPLANTEVWVLDETGLPVPVG
                     SPGELYVSGPCLARGYLGNPALTAERFQPHPFPSHGSHARLYRTGDRGRWSVDGQIEFL
                     GRVDHMVKIRGYRVEPGEVAAALGAHPRVRHCVVTAPRDAAGRADLVAYVVTEGEAPPV
                     GELRSALRERLPEYMVPRTFVFLDELPLTPQGKVDRGRLPAPSGERPDLEVAFEPARPG
                     VEERLAGIWGQVLGVDQIGRHDNFFDLGGDSIRSIQVLGKARDAGLVFGLPELFQAPTL
                     AGLCASVTVDAEGGSHSAATATVTEPFGLVSAEDLARLPEGLEDAYPMAELQLGMVYEM
                     ERDPDRNPYHNVSSLKVGERFEEKPFRRALALVVARHAVLRTSFDLSSFNEPLQLVHAR
                     AALPLTVADLRGVCEEDQRSAIAEHVRTEERNLFPLASAPLCRMSVHVLSDDAFQWTVT
                     DHHAILDGWSLASTLAEITDTYHALLRGEDVTLPPIRSTYRDYIAAERTTLTTTESTTY
                     WHTLLAERPQATLPRWPAETRQSEDTQGRGELSAALPRELCDQLEQLAERVGVPVKSVL
                     LAAHLRALSVAMGATDVVTGLSSNGRLETSGGAEVRGLFLNTVPLRLRLSEGTWLDLVR
                     GVFEAEREMLPHRRYPMSALQRAFGGEPLFETGFVYNHFRQIGELAEGTSAEQGVGDPV
                     DGAGRTHFPLLVAVSREPGHAGMRLELQYDSRELTSQQVALVRDYYVRVLEAMVADPDA
                     SYGQVRVLGDDESSQLGLWAGEGARFPVSSLHEAVVRRAVEAPDEVAVVCGGDSLTYAE
                     LVVRAGRVAEYLKGRGVGVETPVGVCVERSLETVVATLGVLLAGGVYVPLDTAFPADRM
                     RFMLQDVDAGLVLTQGSTAALVPDGPWEVVDLDGVSGPQAQDLDLTLERVAAEVAVGVS
                     TDHACYVIFTSGSTGRPKGTTVAHGNVTRLFEAVRAR"
     CDS_motif       complement(39..98)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0014"
                     /evalue="2.60E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="1721"
                     /protein_start="1701"
                     /score="26.1"
                     /tool="antismash"
                     /translation="CYVIFTSGSTGRPKGTTVAH"
     CDS_motif       complement(312..353)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0013"
                     /evalue="3.50E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="1630"
                     /protein_start="1616"
                     /score="14.0"
                     /tool="antismash"
                     /translation="LGVLLAGGVYVPLD"
     CDS_motif       complement(900..977)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0012"
                     /evalue="2.30E-03"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="1434"
                     /protein_start="1408"
                     /score="10.6"
                     /tool="antismash"
                     /translation="REMLPHRRYPMSALQRAFGGEPLFET"
     aSDomain        complement(1026..1931)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS25320_Condensation_DCL.2
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="3.70E-61"
                     /label="GCN81_RS25320_Condensation_DCL.2"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="1392"
                     /protein_start="1090"
                     /score="198.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHAILDG"
                     /tool="antismash"
                     /translation="EDAYPMAELQLGMVYEMERDPDRNPYHNVSSLKVGERFEEKPFRR
                     ALALVVARHAVLRTSFDLSSFNEPLQLVHARAALPLTVADLRGVCEEDQRSAIAEHVRT
                     EERNLFPLASAPLCRMSVHVLSDDAFQWTVTDHHAILDGWSLASTLAEITDTYHALLRG
                     EDVTLPPIRSTYRDYIAAERTTLTTTESTTYWHTLLAERPQATLPRWPAETRQSEDTQG
                     RGELSAALPRELCDQLEQLAERVGVPVKSVLLAAHLRALSVAMGATDVVTGLSSNGRLE
                     TSGGAEVRGLFLNTVPLRLRL"
     CDS_motif       complement(1461..1523)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0011"
                     /evalue="2.70E-05"
                     /label="C3_DCL_135-156"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="1247"
                     /protein_start="1226"
                     /score="16.8"
                     /tool="antismash"
                     /translation="HHAILDGWSLASTLAEITDTY"
     CDS_motif       complement(1887..1922)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0010"
                     /evalue="5.40E-01"
                     /label="C1_dual_004-017"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="1105"
                     /protein_start="1093"
                     /score="3.9"
                     /tool="antismash"
                     /translation="YPMAELQLGMVY"
     aSDomain        complement(2034..2231)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS25320_PCP.1"
                     /evalue="1.50E-20"
                     /label="GCN81_RS25320_PCP.1"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="1056"
                     /protein_start="990"
                     /score="65.2"
                     /tool="antismash"
                     /translation="EERLAGIWGQVLGVDQIGRHDNFFDLGGDSIRSIQVLGKARDAGL
                     VFGLPELFQAPTLAGLCASVT"
     aSModule        complement(2034..5201)
                     /complete
                     /domains="nrpspksdomains_GCN81_RS25320_Condensation_DCL.1"
                     /domains="nrpspksdomains_GCN81_RS25320_AMP-binding.1"
                     /domains="nrpspksdomains_GCN81_RS25320_PCP.1"
                     /locus_tags="GCN81_RS25320"
                     /monomer_pairings="X -> X"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2532..2594)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0009"
                     /evalue="3.40E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="890"
                     /protein_start="869"
                     /score="32.0"
                     /tool="antismash"
                     /translation="FLGRVDHMVKIRGYRVEPGEV"
     aSDomain        complement(2559..3752)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS25320_AMP-binding.1"
                     /evalue="6.00E-108"
                     /label="GCN81_RS25320_AMP-binding.1"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="881"
                     /protein_start="483"
                     /score="353.0"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="ARATATPERTAVIVGDRSLTYAELDALANRLAHRLRDLGAGPETY
                     IGVCLERSPELIVTLLAVLKAGGAYVPMDPGFPAARLEHMAHQADVRVLLVAPGTADRV
                     PAGPWQTVVLDDTVLVDDGVPREAPENGAGPDNACYTIFTSGSTGLPKGVVTRHRNVTE
                     LLYGGAAMDLREDDTLLQIATVAFDVSTFEIWAPLVAGARLVLAPSGPYETGDLAEWIA
                     RYDVSVLHATASLFALLMDQQPQALDGLRKLLTGSETVSPAHVARAVARHPALEVVNCW
                     GPTETTTFSVCGTFTAENLPAGPLPLGTPLANTEVWVLDETGLPVPVGSPGELYVSGPC
                     LARGYLGNPALTAERFQPHPFPSHGSHARLYRTGDRGRWSVDGQIEFLGRVDHMVKIR"
     CDS_motif       complement(2685..2774)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0008"
                     /evalue="3.70E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="839"
                     /protein_start="809"
                     /score="54.4"
                     /tool="antismash"
                     /translation="PVGSPGELYVSGPCLARGYLGNPALTAERF"
     CDS_motif       complement(3279..3335)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0007"
                     /evalue="3.00E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="641"
                     /protein_start="622"
                     /score="22.9"
                     /tool="antismash"
                     /translation="YTIFTSGSTGLPKGVVTRH"
     CDS_motif       complement(3531..3572)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0006"
                     /evalue="1.00E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="557"
                     /protein_start="543"
                     /score="18.6"
                     /tool="antismash"
                     /translation="LAVLKAGGAYVPMD"
     CDS_motif       complement(4131..4208)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0005"
                     /evalue="1.90E-03"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="357"
                     /protein_start="331"
                     /score="10.9"
                     /tool="antismash"
                     /translation="RAALPHRRYPMPALQRALGGEPLFET"
     aSDomain        complement(4257..5201)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GCN81_RS25320_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="8.00E-59"
                     /label="GCN81_RS25320_Condensation_DCL.1"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="315"
                     /protein_start="0"
                     /score="191.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHAILDG"
                     /tool="antismash"
                     /translation="MYPMAELQLGMVYEMERDPDRNPYLNVTSLRLPGRFDESAFRTAV
                     SKVVRRHPALRTSFALSSLSEPMQLVHAEADLPVVVTDVTGQDSEVQRSVLANHVASER
                     ARGFDTASAPLCRMSVHVLSDDAFQWTVTDHHAILDGWSLASTLAEITDTYHALLQGED
                     VTLPPLRSTYRDYIAAERTALTTTESTTFWHTLLAERPQATLPRWPTDRPGHPSLTGER
                     QEGESHHHDGDTGRGALVSVVPADLVTELETVAGRIGVPLKAVLLAAHLRVLGFVTGGA
                     DVVTGLSSNGRLEETDGSEVRGLFLNSLPFRLRL"
     CDS_motif       complement(4266..4355)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0004"
                     /evalue="2.70E-02"
                     /label="C5_dual_260-291"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="312"
                     /protein_start="282"
                     /score="7.8"
                     /tool="antismash"
                     /translation="VVTGLSSNGRLEETDGSEVRGLFLNSLPFR"
     CDS_motif       complement(4737..4799)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0003"
                     /evalue="2.70E-05"
                     /label="C3_DCL_135-156"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="155"
                     /protein_start="134"
                     /score="16.8"
                     /tool="antismash"
                     /translation="HHAILDGWSLASTLAEITDTY"
     CDS_motif       complement(5025..5132)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0002"
                     /evalue="1.60E-12"
                     /label="C2_DCL_024-062"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="59"
                     /protein_start="23"
                     /score="39.7"
                     /tool="antismash"
                     /translation="YLNVTSLRLPGRFDESAFRTAVSKVVRRHPALRTSF"
     CDS_motif       complement(5163..5198)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GCN81_RS25320_0001"
                     /evalue="5.40E-01"
                     /label="C1_dual_004-017"
                     /locus_tag="GCN81_RS25320"
                     /protein_end="13"
                     /protein_start="1"
                     /score="3.9"
                     /tool="antismash"
                     /translation="YPMAELQLGMVY"
ORIGIN
        1 cgcgcacgga ccgcctcaaa cagccgggtg acgttgccat gcgccacggt ggtgcccttg
       61 gggcggccgg tggaccctga cgtgaagatc acgtagcacg cgtgatccgt actgaccccc
      121 accgcgacct cggccgcgac ccgctccagc gtcagatcca ggtcctgcgc ctgcgggcca
      181 ctgacgccgt cgaggtcgac gacttcccac ggcccgtccg gcaccaacgc agccgtcgac
      241 ccctgcgtca gtacgaggcc agcgtcgacg tcctgcaaca tgaaccgcat gcgatcagcc
      301 gggaaggcgg tgtccagcgg tacgtacaca ccccccgcca gcagcacccc gagcgttgcc
      361 accacggtct ccagcgagcg ctccacgcac acgcccaccg gagtctcaac gccaaccccc
      421 cgccccttca ggtactcggc aacccgcccg gcacgcacca ccagctccgc ataggtcagc
      481 gaatctccgc cgcacaccac cgcgacctca tccggcgcct ccacagcacg acgcaccacc
      541 gcctcatgca gcgacgacac cgggaaccga gcaccctcgc ccgcccacag cccgagctgg
      601 gaagactcat cgtcgccgag cacccgtacc tgcccgtaag aggcgtccgg atcagcgacc
      661 atcgcctcca gcactcgcac gtagtaatca cgcaccaacg cgacttgctg tgacgtcaac
      721 tcgcggctgt cgtactgcaa ctccagccgc atcccagcgt gtccaggctc tcggctcacc
      781 gcgacgagca gagggaagtg ggtccgcccg gcgccgtcta cgggatcgcc cacgccctgc
      841 tccgctgacg tgccttcggc caactcaccg atctgacgga agtggttgta gacgaatcca
      901 gtctcgaaca gcggctcccc accgaaggcg cgctgcagcg cggacatcgg ataccggcgg
      961 tggggcagca tctcccgctc cgcctcgaac accccccgca ccaggtccag ccaggtcccc
     1021 tccgacagtc gcagccgcag gggcaccgtg ttgaggaaca ggccgcgtac ctcggcaccg
     1081 ccgctcgtct ccagacggcc gttggaagag aggcccgtca ccacgtccgt ggcgcccata
     1141 gccacgctca gggcccgcag gtgcgccgcc agcagcaccg acttgaccgg taccccaact
     1201 cgctccgcca actgctccag ctggtcgcac agctcacgcg gcagtgctgc ggagagctcg
     1261 ccgcgcccct gcgtgtcctc actctgccgt gtctcggcgg gccagcgggg gagggttgcc
     1321 tgaggtcgtt cggcgagcag ggtgtgccag taggtggtgc tttcggtggt ggtgagggtg
     1381 gtgcgttcgg cggcgatgta gtcgcggtag gtggagcgga ttgggggcag ggtgacgtct
     1441 tcgccgcgga ggagtgcgtg gtaggtgtcg gtgatttcgg cgagggtgga ggcgaggctc
     1501 cagccgtcga ggatggcgtg gtggtcggtg acggtccact ggaaggcgtc atcggacagt
     1561 acgtgcacac tcatccggca caacggagcc gacgccagcg ggaaaaggtt tcgctcctcg
     1621 gtccggacgt gttccgcgat cgctgagcgc tggtcctcct cacacacacc tcgtaggtcg
     1681 gcgacggtca gcggcagcgc cgcccgcgcg tgcaccagct ggagcggctc gttgaagctg
     1741 ctgaggtcga aggaggttcg caggaccgcg tgccgcgcca ccaccagggc gagcgcgcgc
     1801 cggaagggtt tctcctcgaa ccgttcgccg accttcagtg agctcacgtt gtggtacggg
     1861 ttgcggtcgg ggtcccgttc catttcgtag accatgccga gttggagttc ggccatgggg
     1921 taggcgtctt ccaggccttc cggcaaccgt gccaggtcct cggctgacac cagcccgaac
     1981 ggctcagtga cagtggcagt ggcggcggag tgggacccac cctcagcgtc cacggtcacc
     2041 gacgcgcaga gcccggccag ggtcggcgcc tggaacagct ccggcagtcc gaagaccagc
     2101 ccggcgtccc gcgccttgcc caggacttgg atgctgcgga tggagtcgcc gccgaggtcg
     2161 aagaagttgt cgtggcggcc gatctggtcc acgcccagga cctgacccca gatcccggcc
     2221 aaccgctcct ccacacccgg ccgggcaggt tcgaacgcca cctccaggtc cgggcgttca
     2281 ccggaaggtg ccgggagccg accgcggtcc accttgcctt gcggggtcag cggcagctcg
     2341 tccaggaaga cgaacgtacg cggcaccatg tactccggca gccgctctcg cagcgcggag
     2401 cgcaactcgc ctaccggggg cgcttccccc tcggtcacca cgtacgcgac caggtcggcc
     2461 cgccccgcgg cgtcccgtgg tgccgtcacc acgcagtgcc gcacgcgggg gtgggcgccg
     2521 agcgccgcgg cgacctcgcc cggctcgacc cggtagccgc ggatcttgac catgtgatcg
     2581 acacggccca ggaactcgat ctggccgtcc accgaccacc ggccccggtc acctgtgcgg
     2641 tagagacgag cgtgggaacc gtgggacggg aaggggtgcg gctggaaccg ctcggcggtc
     2701 agcgccgggt tgcccaggta gccgcgggcc aggcacggcc cggatacgta cagctcaccg
     2761 gggctgccga caggaacggg gaggcccgtc tcgtccagca cccagacctc ggtgttggcg
     2821 agaggagtgc cgaggggcag cgggccagcg ggcagattct ccgccgtgaa ggtgccgcag
     2881 accgagaacg tggtggtttc cgtcggaccc cagcagttca ccacctccag cgcggggtgg
     2941 cgggccacgg cgcgcgcgac gtgcgccggc gagacggtct cactgccggt gaggagctta
     3001 cgcagcccgt cgagggcctg cggctgctgg tccatcagca gcgcgaacag ggaagccgtc
     3061 gcgtgcagca cgcttacgtc ataccgtgcg atccactcag ccaagtcgcc cgtttcgtaa
     3121 ggcccggagg gagccaggac caaccgggct ccagccacca gcggtgccca gatctcgaaa
     3181 gtggagacgt cgaacgcgac cgtggcgatc tgcagaagcg tgtcgtcctc acgaaggtcc
     3241 atcgccgcac cgccgtacag cagctcggtg acgttgcggt gccgggtcac cacgcccttc
     3301 ggcaggccgg tcgagccgga ggtgaagatg gtgtaacagg cgttgtccgg tcccgccccg
     3361 ttctccgggg cctcgcgcgg cacaccgtca tccaccagca cggtgtcgtc cagcaccacg
     3421 gtctgccaag gtcccgcggg gacgcgatcc gccgtgccag gggcgacgag gagcactcgt
     3481 acgtccgcct gatgcgccat gtgctccagc cgcgcggcgg ggaaacccgg gtccatgggc
     3541 acgtaggcgc caccagcctt gagtacggcc agcagggtga cgatcagttc cggggagcgc
     3601 tcgaggcaga cgccgatata cgtctcaggt cccgcaccca ggtcacggag gcggtgagcg
     3661 agccggttcg ccagagcgtc cagctcggcg taggtgaggc tgcggtcacc aacaatgaca
     3721 gccgtacgct caggcgtcgc ggtggcacgc gccgccacca gctcgtgcac gggggtgtcg
     3781 ggcaccggcg tggcggtgtc attccagtcg gcgagcagcg cgtgttcgga gctgctcagc
     3841 agatcggtct gcctgtggtc ggcgtcggga gtccccgcca gcgccgtcag ggcgcgcaga
     3901 tggtagtcgc gaagcaagcg cacctgctcg gcagcgaact gccgcgcgtc gtattccagt
     3961 tccacccgca tcccggtctc gccgggctcg cggctcatgg cgacgtgcaa ggggaagctg
     4021 gtacgggcga caccagccgc tatctcgtcg tacgactcgg agatcgagac cgccgcccga
     4081 ccactgtccg ccagatcttc gacgtggcgg aagtggttgt agacgaatcc ggtctcgaac
     4141 agcggctccc cgcccagtgc gcgctggagc gcgggcatgg ggtacctacg gtgcggcaag
     4201 gccgctcgct cggcggcgaa cacggcgcgg gccaggtcgg cccaactgcc gtcggacagc
     4261 cgcaaccgga atggaaggga gttgaggaac agaccgcgga cttccgaccc gtcggtttcc
     4321 tccagacgcc cgttggacga cagaccggtc accacgtccg caccaccggt gacgaagccc
     4381 agcacccgca ggtgcgcggc cagcagtacg gccttcagtg gtacaccgat acgtcccgca
     4441 accgtctcga gctcggtcac caggtccgcg ggcaccacac tgaccaacgc gccgcgaccc
     4501 gtgtctccgt cgtggtggtg cgactcgcct tcctggcgct caccggtgag ggacgggtgc
     4561 ccgggccggt cggtgggcca gcgggggagg gttgcctgag gtcgttcggc gagcagggtg
     4621 tgccagaagg tggtgctttc ggtggtggtg agggcggtgc gttcggcggc gatgtagtcg
     4681 cggtaggtgg agcgtagtgg gggcagggtg acgtcttcgc cttgtaggag tgcgtggtag
     4741 gtgtcggtga tttcggcgag ggtggaggcg aggctccagc cgtcgaggat ggcgtggtgg
     4801 tcggtgacgg tccactggaa ggcgtcatcg gacagtacgt gcacactcat ccggcacaac
     4861 ggagccgagg ccgtgtcgaa gccgcgggcg cgctcgctcg ccacatggtt cgcgagcacc
     4921 gaacgctgaa cctcggagtc ctggcccgtc acatcggtga cgacgacggg gaggtctgcc
     4981 tcggcgtgta cgagctgcat cggctcgctc aggctgctga gggcaaacga ggtccgcagc
     5041 gctgggtgcc gccgcaccac cttggacaca gctgtacgga acgcggactc gtcgaagcgt
     5101 ccaggcagcc gcagtgaggt gacgttcagg tacgggttgc ggtcggggtc ccgttccatt
     5161 tcgtagacca tgccgagttg gagttcggcc atggggtagg c
//
